FI103129B1 - Saccaharomyces cerevisiae -kanta ja menetelmä S. cerevisiaelle heterologisen proteiinin tuottamiseksi - Google Patents

Saccaharomyces cerevisiae -kanta ja menetelmä S. cerevisiaelle heterologisen proteiinin tuottamiseksi

Info

Publication number
FI103129B1
FI103129B1 FI892114A FI892114A FI103129B1 FI 103129 B1 FI103129 B1 FI 103129B1 FI 892114 A FI892114 A FI 892114A FI 892114 A FI892114 A FI 892114A FI 103129 B1 FI103129 B1 FI 103129B1
Authority
FI
Finland
Prior art keywords
cerevisiae
producing
saccaharomyces
protein heterologous
strain
Prior art date
Application number
FI892114A
Other languages
English (en)
Swedish (sv)
Other versions
FI103129B (fi
FI892114A (fi
FI892114A0 (fi
Inventor
Hansjoerg Treichler
Kenji Takabayashi
Dieter Heinrich Wolf
Jutta Heim
Original Assignee
Novartis Ag
Ucp Gen Pharma Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB888810524A external-priority patent/GB8810524D0/en
Priority claimed from GB888812627A external-priority patent/GB8812627D0/en
Priority claimed from GB898907110A external-priority patent/GB8907110D0/en
Application filed by Novartis Ag, Ucp Gen Pharma Ag filed Critical Novartis Ag
Publication of FI892114A0 publication Critical patent/FI892114A0/fi
Publication of FI892114A publication Critical patent/FI892114A/fi
Application granted granted Critical
Publication of FI103129B1 publication Critical patent/FI103129B1/fi
Publication of FI103129B publication Critical patent/FI103129B/fi

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
FI892114A 1988-05-04 1989-05-03 Saccaharomyces cerevisiae -kanta ja menetelmä S. cerevisiaelle heterol ogisen proteiinin tuottamiseksi FI103129B (fi)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB8810524 1988-05-04
GB888810524A GB8810524D0 (en) 1988-05-04 1988-05-04 Improvements in production of polypeptides
GB888812627A GB8812627D0 (en) 1988-05-27 1988-05-27 Improvements in production of polypeptides
GB8812627 1988-05-27
GB898907110A GB8907110D0 (en) 1988-05-04 1989-03-29 Improvements in the production of polypeptides
GB8907110 1989-03-29

Publications (4)

Publication Number Publication Date
FI892114A0 FI892114A0 (fi) 1989-05-03
FI892114A FI892114A (fi) 1989-11-05
FI103129B1 true FI103129B1 (fi) 1999-04-30
FI103129B FI103129B (fi) 1999-04-30

Family

ID=27263890

Family Applications (1)

Application Number Title Priority Date Filing Date
FI892114A FI103129B (fi) 1988-05-04 1989-05-03 Saccaharomyces cerevisiae -kanta ja menetelmä S. cerevisiaelle heterol ogisen proteiinin tuottamiseksi

Country Status (16)

Country Link
EP (1) EP0341215B1 (fi)
JP (1) JPH0824565B2 (fi)
AT (1) ATE124086T1 (fi)
AU (1) AU614121B2 (fi)
CA (1) CA1340016C (fi)
DE (1) DE68923123T2 (fi)
DK (1) DK175701B1 (fi)
ES (1) ES2072918T3 (fi)
FI (1) FI103129B (fi)
HU (1) HU218731B (fi)
IE (1) IE66044B1 (fi)
IL (1) IL90170A (fi)
MX (1) MX169508B (fi)
NO (1) NO180593C (fi)
NZ (1) NZ228955A (fi)
PT (1) PT90436B (fi)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8809860D0 (en) * 1988-04-26 1988-06-02 Ciba Geigy Ag Process for production of polypeptides
FR2645174B1 (fr) * 1989-03-31 1994-01-07 Transgene Sa Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant
FR2645175B1 (fr) * 1989-03-31 1994-02-18 Transgene Sa Souche de saccharomyces cerevisiaeproductrice d'une proteine heterologue et procede de preparation de ladite proteine heterologue par fermentation de ladite souche
US5879926A (en) * 1989-03-31 1999-03-09 Transgene S.A. Yeast strains for the production of mature heterologous proteins, especially hirudin
WO1991008229A1 (de) * 1989-12-01 1991-06-13 Basf Aktiengesellschaft Hirudinpolyalkylenglykolkonjugate
US5580734A (en) * 1990-07-13 1996-12-03 Transkaryotic Therapies, Inc. Method of producing a physical map contigous DNA sequences
DE69114500T2 (de) * 1990-07-13 1996-06-13 Transkaryotic Therapies Inc Verfahren zum absuchen einer bibliothek.
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
EP0548267A4 (en) * 1990-09-04 1994-11-23 Salk Inst Biotech Ind Production of insulin-like growth factor-1 in methylotrophic yeast cells
ATE140929T1 (de) 1990-11-08 1996-08-15 Japan Energy Corp Hirudinmutante, deren herstellung, antikoagulans, sekretorischer vektor, durch besagten vektor transformierter mikroorganismus und herstellung eines produkts durch besagten mikroorganismus
EP0501914A1 (en) * 1991-02-25 1992-09-02 Ciba-Geigy Ag Improved yeast vectors
DE69232666T2 (de) * 1991-04-01 2003-03-20 Merck & Co Inc Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung
HU214698B (hu) * 1992-04-09 1998-06-29 Gyógyszerkutató Intézet Kft. Eljárás rekombináns deszulfátó-hirudin HV-1 peptidek előállítására
EP0592358B1 (en) * 1992-09-04 2000-10-11 Novartis AG Process for the production of protease inhibitors
TW282490B (fi) * 1992-12-15 1996-08-01 Ciba Geigy Ag
EP0663009A1 (en) * 1993-08-04 1995-07-19 Novartis AG High molecular weight desulphatohirudin
DE4404168A1 (de) * 1994-02-10 1995-08-17 Hoechst Ag Hirudinderivate und Verfahren zu deren Herstellung
ATE207538T1 (de) * 1995-02-09 2001-11-15 Behringwerke Ag Verfahren zur produktion von proteinen
AU4940096A (en) * 1995-03-20 1996-10-08 Novo Nordisk A/S Host cell expressing reduced levels of a metalloprotease and methods using the host cell in protein production
DE19529997C1 (de) * 1995-08-16 1997-04-10 Hoechst Ag Verfahren zur Inaktivierung von Carboxypeptidase Y in hirudinhaltigen Kulturbrühen
WO1997012045A1 (en) * 1995-09-27 1997-04-03 Chiron Corporation Method of making a protease deficient host
DE69625623T2 (de) 1996-01-31 2003-11-06 Sumitomo Bakelite Co Verfahren zur Herstellung von in Epoxyharz eingekapselter Halbleitervorrichtung
FR2768743B1 (fr) * 1997-09-23 2001-09-14 Bio Merieux Procede de lyse de micro-organisme
GB2338236B (en) * 1998-06-13 2003-04-09 Aea Technology Plc Microbiological cell processing
US7795205B2 (en) 2004-04-12 2010-09-14 Canyon Pharmaceuticals, Inc. Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor
CN102639559B (zh) 2009-11-25 2015-04-29 诺沃—诺迪斯克有限公司 用于制备多肽的方法
WO2019073954A1 (ja) 2017-10-10 2019-04-18 味の素株式会社 タンパク質の製造法
EP4166647A1 (en) 2020-06-11 2023-04-19 Ajinomoto Co., Inc. Method for producing protein

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3342139A1 (de) * 1983-11-22 1985-05-30 Ciba-Geigy Ag, Basel Desulfatohirudine, verfahren zu ihrer herstellung und pharmazeutische mittel
JPS62224284A (ja) * 1986-03-25 1987-10-02 Agency Of Ind Science & Technol 微生物
GB8809860D0 (en) * 1988-04-26 1988-06-02 Ciba Geigy Ag Process for production of polypeptides
GB8817161D0 (en) * 1988-07-19 1988-08-24 Ciba Geigy Ag Modified proteins
GB8817160D0 (en) * 1988-07-19 1988-08-24 Ciba Geigy Ag Novel proteins

Also Published As

Publication number Publication date
AU3382889A (en) 1989-11-09
HUT54210A (en) 1991-01-28
EP0341215B1 (en) 1995-06-21
MX169508B (es) 1993-07-08
DE68923123D1 (de) 1995-07-27
PT90436A (pt) 1989-11-30
JPH0824565B2 (ja) 1996-03-13
DK175701B1 (da) 2005-01-24
NZ228955A (en) 1991-06-25
CA1340016C (en) 1998-08-25
IL90170A (en) 1995-12-31
NO891837D0 (no) 1989-05-03
IE66044B1 (en) 1995-12-13
IL90170A0 (en) 1989-12-15
DK217589D0 (da) 1989-05-03
ATE124086T1 (de) 1995-07-15
ES2072918T3 (es) 1995-08-01
PT90436B (pt) 1994-11-30
EP0341215A3 (en) 1992-01-15
JPH02104279A (ja) 1990-04-17
NO180593B (no) 1997-02-03
AU614121B2 (en) 1991-08-22
FI103129B (fi) 1999-04-30
FI892114A (fi) 1989-11-05
NO180593C (no) 1997-05-14
FI892114A0 (fi) 1989-05-03
DE68923123T2 (de) 1995-11-30
EP0341215A2 (en) 1989-11-08
DK217589A (da) 1989-11-05
IE891451L (en) 1989-11-04
HU218731B (hu) 2000-11-28
NO891837L (no) 1989-11-06

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FR2645174B1 (fr) Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant
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Legal Events

Date Code Title Description
GB Transfer or assigment of application

Owner name: NOVARTIS AG

FG Patent granted

Owner name: UCP GEN-PHARMA AG

Owner name: NOVARTIS AG

MA Patent expired