DK175701B1 - Fremgangsmåde til fremstilling af polypeptider - Google Patents

Fremgangsmåde til fremstilling af polypeptider Download PDF

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Publication number
DK175701B1
DK175701B1 DK198902175A DK217589A DK175701B1 DK 175701 B1 DK175701 B1 DK 175701B1 DK 198902175 A DK198902175 A DK 198902175A DK 217589 A DK217589 A DK 217589A DK 175701 B1 DK175701 B1 DK 175701B1
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DK
Denmark
Prior art keywords
yeast
dna
promoter
gene
plasmid
Prior art date
Application number
DK198902175A
Other languages
Danish (da)
English (en)
Other versions
DK217589A (da
DK217589D0 (da
Inventor
Jutta Heim
Kenji Takabayashi
Hansjoerg Treichler
Dieter Heinrich Wolf
Original Assignee
Novartis Ag
Ucp Gen Pharma Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB888810524A external-priority patent/GB8810524D0/en
Priority claimed from GB888812627A external-priority patent/GB8812627D0/en
Priority claimed from GB898907110A external-priority patent/GB8907110D0/en
Application filed by Novartis Ag, Ucp Gen Pharma Ag filed Critical Novartis Ag
Publication of DK217589D0 publication Critical patent/DK217589D0/da
Publication of DK217589A publication Critical patent/DK217589A/da
Application granted granted Critical
Publication of DK175701B1 publication Critical patent/DK175701B1/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Liquid Crystal Substances (AREA)
  • Enzymes And Modification Thereof (AREA)
DK198902175A 1988-05-04 1989-05-03 Fremgangsmåde til fremstilling af polypeptider DK175701B1 (da)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB888810524A GB8810524D0 (en) 1988-05-04 1988-05-04 Improvements in production of polypeptides
GB8810524 1988-05-04
GB8812627 1988-05-27
GB888812627A GB8812627D0 (en) 1988-05-27 1988-05-27 Improvements in production of polypeptides
GB898907110A GB8907110D0 (en) 1988-05-04 1989-03-29 Improvements in the production of polypeptides
GB8907110 1989-03-29

Publications (3)

Publication Number Publication Date
DK217589D0 DK217589D0 (da) 1989-05-03
DK217589A DK217589A (da) 1989-11-05
DK175701B1 true DK175701B1 (da) 2005-01-24

Family

ID=27263890

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198902175A DK175701B1 (da) 1988-05-04 1989-05-03 Fremgangsmåde til fremstilling af polypeptider

Country Status (16)

Country Link
EP (1) EP0341215B1 (fi)
JP (1) JPH0824565B2 (fi)
AT (1) ATE124086T1 (fi)
AU (1) AU614121B2 (fi)
CA (1) CA1340016C (fi)
DE (1) DE68923123T2 (fi)
DK (1) DK175701B1 (fi)
ES (1) ES2072918T3 (fi)
FI (1) FI103129B (fi)
HU (1) HU218731B (fi)
IE (1) IE66044B1 (fi)
IL (1) IL90170A (fi)
MX (1) MX169508B (fi)
NO (1) NO180593C (fi)
NZ (1) NZ228955A (fi)
PT (1) PT90436B (fi)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8809860D0 (en) * 1988-04-26 1988-06-02 Ciba Geigy Ag Process for production of polypeptides
US5879926A (en) * 1989-03-31 1999-03-09 Transgene S.A. Yeast strains for the production of mature heterologous proteins, especially hirudin
FR2645175B1 (fr) * 1989-03-31 1994-02-18 Transgene Sa Souche de saccharomyces cerevisiaeproductrice d'une proteine heterologue et procede de preparation de ladite proteine heterologue par fermentation de ladite souche
FR2645174B1 (fr) * 1989-03-31 1994-01-07 Transgene Sa Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant
JP3187044B2 (ja) * 1989-12-01 2001-07-11 ビーエーエスエフ アクチェンゲゼルシャフト ヒルジン突然変異蛋白質及びヒルジンポリアルキレングリコール複合体
PT98310B (pt) * 1990-07-13 1999-01-29 Transkaryotic Therapies Inc Processo de recombinacao homologa para identificacao e isolamento dum fragmento pretendido de adn a partir de uma biblioteca de fragmentos
US5580734A (en) * 1990-07-13 1996-12-03 Transkaryotic Therapies, Inc. Method of producing a physical map contigous DNA sequences
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
WO1992004363A1 (en) * 1990-09-04 1992-03-19 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of insulin-like growth factor-1 in methylotrophic yeast cells
DK0511393T3 (da) 1990-11-08 1996-12-09 Japan Energy Corp Hirudin-mutant, fremstilling af denne, antikoagulant, sekretorisk vektor, mikroorganisme transformeret af vektoren og fremstilling af produkt ud fra mikroorganismen
SG84484A1 (en) * 1991-02-25 2001-11-20 Ciba Geigy Ag Improved yeast vectors
EP0578746B1 (en) * 1991-04-01 2002-07-03 Merck & Co., Inc. GENES WHICH INFLUENCE $i(PICHIA) PROTEOLYTIC ACTIVITY, AND USES THEREFOR
HU214698B (hu) * 1992-04-09 1998-06-29 Gyógyszerkutató Intézet Kft. Eljárás rekombináns deszulfátó-hirudin HV-1 peptidek előállítására
ES2152943T3 (es) * 1992-09-04 2001-02-16 Novartis Ag Procedimiento para la produccion de inhibidores de proteasas.
TW282490B (fi) * 1992-12-15 1996-08-01 Ciba Geigy Ag
KR950703645A (ko) * 1993-08-04 1995-09-20 베르너 발데크 고분자량 데술페이토히루딘(High Molecular Weight Desulphatohirudin)
DE4404168A1 (de) * 1994-02-10 1995-08-17 Hoechst Ag Hirudinderivate und Verfahren zu deren Herstellung
US5981227A (en) * 1995-02-09 1999-11-09 Movartis Ag Process for the production of proteins
AU4940096A (en) * 1995-03-20 1996-10-08 Novo Nordisk A/S Host cell expressing reduced levels of a metalloprotease and methods using the host cell in protein production
DE19529997C1 (de) 1995-08-16 1997-04-10 Hoechst Ag Verfahren zur Inaktivierung von Carboxypeptidase Y in hirudinhaltigen Kulturbrühen
AU7112796A (en) * 1995-09-27 1997-04-17 Chiron Corporation Method of making a protease deficient host
EP0787569B1 (en) 1996-01-31 2002-10-02 Sumitomo Bakelite Company Limited Method of producing epoxy resin-encapsulated semiconductor device
FR2768743B1 (fr) 1997-09-23 2001-09-14 Bio Merieux Procede de lyse de micro-organisme
GB2338236B (en) * 1998-06-13 2003-04-09 Aea Technology Plc Microbiological cell processing
US7795205B2 (en) 2004-04-12 2010-09-14 Canyon Pharmaceuticals, Inc. Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor
EP2504355B1 (en) 2009-11-25 2015-07-22 Novo Nordisk A/S Method for making polypeptides
CN111542615B (zh) * 2017-10-10 2023-11-17 味之素株式会社 蛋白质的制造方法
JPWO2021251383A1 (fi) 2020-06-11 2021-12-16

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3342139A1 (de) * 1983-11-22 1985-05-30 Ciba-Geigy Ag, Basel Desulfatohirudine, verfahren zu ihrer herstellung und pharmazeutische mittel
JPS62224284A (ja) * 1986-03-25 1987-10-02 Agency Of Ind Science & Technol 微生物
GB8809860D0 (en) * 1988-04-26 1988-06-02 Ciba Geigy Ag Process for production of polypeptides
GB8817160D0 (en) * 1988-07-19 1988-08-24 Ciba Geigy Ag Novel proteins
GB8817161D0 (en) * 1988-07-19 1988-08-24 Ciba Geigy Ag Modified proteins

Also Published As

Publication number Publication date
NZ228955A (en) 1991-06-25
IE66044B1 (en) 1995-12-13
DK217589A (da) 1989-11-05
PT90436B (pt) 1994-11-30
CA1340016C (en) 1998-08-25
HUT54210A (en) 1991-01-28
IE891451L (en) 1989-11-04
FI103129B1 (fi) 1999-04-30
AU3382889A (en) 1989-11-09
JPH0824565B2 (ja) 1996-03-13
NO891837L (no) 1989-11-06
PT90436A (pt) 1989-11-30
EP0341215B1 (en) 1995-06-21
HU218731B (hu) 2000-11-28
IL90170A0 (en) 1989-12-15
AU614121B2 (en) 1991-08-22
FI892114A (fi) 1989-11-05
EP0341215A2 (en) 1989-11-08
ATE124086T1 (de) 1995-07-15
MX169508B (es) 1993-07-08
DE68923123D1 (de) 1995-07-27
NO180593B (no) 1997-02-03
EP0341215A3 (en) 1992-01-15
IL90170A (en) 1995-12-31
NO180593C (no) 1997-05-14
ES2072918T3 (es) 1995-08-01
NO891837D0 (no) 1989-05-03
JPH02104279A (ja) 1990-04-17
DE68923123T2 (de) 1995-11-30
FI892114A0 (fi) 1989-05-03
DK217589D0 (da) 1989-05-03
FI103129B (fi) 1999-04-30

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