DE69114500T2 - Verfahren zum absuchen einer bibliothek. - Google Patents

Verfahren zum absuchen einer bibliothek.

Info

Publication number
DE69114500T2
DE69114500T2 DE69114500T DE69114500T DE69114500T2 DE 69114500 T2 DE69114500 T2 DE 69114500T2 DE 69114500 T DE69114500 T DE 69114500T DE 69114500 T DE69114500 T DE 69114500T DE 69114500 T2 DE69114500 T2 DE 69114500T2
Authority
DE
Germany
Prior art keywords
homologous
methods
scaning
library
recombination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69114500T
Other languages
English (en)
Other versions
DE69114500D1 (de
Inventor
Douglas Treco
Allan Miller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shire Human Genetics Therapies Inc
Original Assignee
Transkaryotic Therapies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transkaryotic Therapies Inc filed Critical Transkaryotic Therapies Inc
Publication of DE69114500D1 publication Critical patent/DE69114500D1/de
Application granted granted Critical
Publication of DE69114500T2 publication Critical patent/DE69114500T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
DE69114500T 1990-07-13 1991-07-12 Verfahren zum absuchen einer bibliothek. Expired - Fee Related DE69114500T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55218390A 1990-07-13 1990-07-13
PCT/US1991/004926 WO1992001069A1 (en) 1990-07-13 1991-07-12 Library screening method

Publications (2)

Publication Number Publication Date
DE69114500D1 DE69114500D1 (de) 1995-12-14
DE69114500T2 true DE69114500T2 (de) 1996-06-13

Family

ID=24204264

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69114500T Expired - Fee Related DE69114500T2 (de) 1990-07-13 1991-07-12 Verfahren zum absuchen einer bibliothek.

Country Status (16)

Country Link
US (1) US5783385A (de)
EP (1) EP0539490B1 (de)
JP (1) JPH05508547A (de)
KR (1) KR930701625A (de)
AT (1) ATE130049T1 (de)
AU (2) AU8299191A (de)
CA (1) CA2086092A1 (de)
DE (1) DE69114500T2 (de)
ES (1) ES2080957T3 (de)
IE (1) IE63212B1 (de)
IL (1) IL98822A0 (de)
MX (1) MX9100215A (de)
MY (1) MY106504A (de)
NZ (1) NZ238957A (de)
PT (1) PT98310B (de)
WO (1) WO1992001069A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992001069A1 (en) * 1990-07-13 1992-01-23 Transkaryotic Therapies, Inc. Library screening method
MX9206979A (es) * 1991-12-04 1993-07-01 Novo Nordisk As Metodo de clonacion de proteinas en levaduras
FR2689905B1 (fr) * 1992-04-13 1995-07-13 Orsan Procede de clonage d'une sequence d'adn codant pour une nadph cytochrome p450 reductase et sequences d'adn codant pour une nadph-cytochrome p450 reductase, notamment de plantes.
US5792632A (en) * 1992-05-05 1998-08-11 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
US5643763A (en) * 1994-11-04 1997-07-01 Genpharm International, Inc. Method for making recombinant yeast artificial chromosomes by minimizing diploid doubling during mating
US5811381A (en) * 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
US7883872B2 (en) * 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
ES2312179T3 (es) 1996-12-06 2009-02-16 Amgen Inc. Terapia combinada que utiliza un inhibidor del il-1 para el tratamiento de enfermedades mediadas por el il-1.
US6740503B1 (en) 1997-09-26 2004-05-25 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
US6897066B1 (en) * 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
ES2237159T3 (es) * 1998-10-06 2005-07-16 Mark Aaron Emalfarb Sistema de transformacion en el campo de los hongos micoticos filamentosos en chrysosporium.
US6867189B2 (en) * 2001-07-26 2005-03-15 Genset S.A. Use of adipsin/complement factor D in the treatment of metabolic related disorders
GB0329722D0 (en) * 2003-12-23 2004-01-28 Delta Biotechnology Ltd Modified plasmid and use thereof
GB0329681D0 (en) * 2003-12-23 2004-01-28 Delta Biotechnology Ltd Gene expression technique
EP1732653A2 (de) * 2004-03-05 2006-12-20 Alumina Micro LLC Selektives bonden zur herstellung eines mikroventils
AU2005299413A1 (en) 2004-10-22 2006-05-04 Revivicor, Inc. Ungulates with genetically modified immune systems
EP2330200B1 (de) 2004-12-23 2017-04-05 Albumedix A/S Genexpressionstechnik
WO2008073914A2 (en) 2006-12-10 2008-06-19 Dyadic International Inc. Expression and high-throughput screening of complex expressed dna libraries in filamentous fungi
US9862956B2 (en) 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
CA2736661A1 (en) * 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
WO2010051288A1 (en) 2008-10-27 2010-05-06 Revivicor, Inc. Immunocompromised ungulates

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4038143A (en) * 1973-10-29 1977-07-26 The Regents Of The University Of Michigan Test kit for the genetic detection of microorganisms
US4745057A (en) * 1984-05-18 1988-05-17 Eli Lilly And Company Method, vectors and transformants for high expression of heterologous polypeptides in yeast
CA1293460C (en) * 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US4818700A (en) * 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
US4889806A (en) * 1987-04-15 1989-12-26 Washington University Large DNA cloning system based on yeast artificial chromosomes
AU614121B2 (en) * 1988-05-04 1991-08-22 Novartis Ag Improvements in the production of polypeptides
GB8920211D0 (en) * 1989-09-07 1989-10-18 Ici Plc Diagnostic method
GB8921791D0 (en) * 1989-09-27 1989-11-08 Imp Cancer Res Tech Disease gene
US5580734A (en) * 1990-07-13 1996-12-03 Transkaryotic Therapies, Inc. Method of producing a physical map contigous DNA sequences
WO1992001069A1 (en) * 1990-07-13 1992-01-23 Transkaryotic Therapies, Inc. Library screening method
CA2114713A1 (en) * 1991-08-02 1993-02-18 Douglas A. Treco Library screening method

Also Published As

Publication number Publication date
ATE130049T1 (de) 1995-11-15
IL98822A0 (en) 1992-07-15
ES2080957T3 (es) 1996-02-16
NZ238957A (en) 1994-04-27
CA2086092A1 (en) 1992-01-14
US5783385A (en) 1998-07-21
AU8299191A (en) 1992-02-04
MX9100215A (es) 1992-02-28
PT98310B (pt) 1999-01-29
EP0539490A1 (de) 1993-05-05
JPH05508547A (ja) 1993-12-02
AU4449396A (en) 1996-06-20
MY106504A (en) 1995-06-30
DE69114500D1 (de) 1995-12-14
IE63212B1 (en) 1995-04-05
PT98310A (pt) 1992-05-29
IE912470A1 (en) 1992-01-15
KR930701625A (ko) 1993-06-12
AU689015B2 (en) 1998-03-19
EP0539490B1 (de) 1995-11-08
WO1992001069A1 (en) 1992-01-23

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee