IL90170A - Method and transformed yeast strain which lacks carboxypeptidase yscalpha activity for the production of polypeptides having a non-basic C-terminus - Google Patents
Method and transformed yeast strain which lacks carboxypeptidase yscalpha activity for the production of polypeptides having a non-basic C-terminusInfo
- Publication number
- IL90170A IL90170A IL9017089A IL9017089A IL90170A IL 90170 A IL90170 A IL 90170A IL 9017089 A IL9017089 A IL 9017089A IL 9017089 A IL9017089 A IL 9017089A IL 90170 A IL90170 A IL 90170A
- Authority
- IL
- Israel
- Prior art keywords
- yeast
- yeast strain
- dna
- activity
- desulphatohirudin
- Prior art date
Links
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- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Liquid Crystal Substances (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB888810524A GB8810524D0 (en) | 1988-05-04 | 1988-05-04 | Improvements in production of polypeptides |
| GB888812627A GB8812627D0 (en) | 1988-05-27 | 1988-05-27 | Improvements in production of polypeptides |
| GB898907110A GB8907110D0 (en) | 1988-05-04 | 1989-03-29 | Improvements in the production of polypeptides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL90170A0 IL90170A0 (en) | 1989-12-15 |
| IL90170A true IL90170A (en) | 1995-12-31 |
Family
ID=27263890
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL9017089A IL90170A (en) | 1988-05-04 | 1989-05-03 | Method and transformed yeast strain which lacks carboxypeptidase yscalpha activity for the production of polypeptides having a non-basic C-terminus |
Country Status (16)
| Country | Link |
|---|---|
| EP (1) | EP0341215B1 (de) |
| JP (1) | JPH0824565B2 (de) |
| AT (1) | ATE124086T1 (de) |
| AU (1) | AU614121B2 (de) |
| CA (1) | CA1340016C (de) |
| DE (1) | DE68923123T2 (de) |
| DK (1) | DK175701B1 (de) |
| ES (1) | ES2072918T3 (de) |
| FI (1) | FI103129B (de) |
| HU (1) | HU218731B (de) |
| IE (1) | IE66044B1 (de) |
| IL (1) | IL90170A (de) |
| MX (1) | MX169508B (de) |
| NO (1) | NO180593C (de) |
| NZ (1) | NZ228955A (de) |
| PT (1) | PT90436B (de) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8809860D0 (en) * | 1988-04-26 | 1988-06-02 | Ciba Geigy Ag | Process for production of polypeptides |
| FR2645175B1 (fr) * | 1989-03-31 | 1994-02-18 | Transgene Sa | Souche de saccharomyces cerevisiaeproductrice d'une proteine heterologue et procede de preparation de ladite proteine heterologue par fermentation de ladite souche |
| FR2645174B1 (fr) * | 1989-03-31 | 1994-01-07 | Transgene Sa | Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant |
| US5879926A (en) * | 1989-03-31 | 1999-03-09 | Transgene S.A. | Yeast strains for the production of mature heterologous proteins, especially hirudin |
| DE59010205D1 (de) * | 1989-12-01 | 1996-04-18 | Basf Ag | Hirudin-Muteine und deren Polyalkylenglykolkonjugate |
| US5580734A (en) * | 1990-07-13 | 1996-12-03 | Transkaryotic Therapies, Inc. | Method of producing a physical map contigous DNA sequences |
| PT98310B (pt) * | 1990-07-13 | 1999-01-29 | Transkaryotic Therapies Inc | Processo de recombinacao homologa para identificacao e isolamento dum fragmento pretendido de adn a partir de uma biblioteca de fragmentos |
| CA2090969C (en) * | 1990-09-04 | 2001-08-21 | Russell Arthur Brierley | Production of insulin-like growth factor-1 in methylotrophic yeast cells |
| US5612198A (en) * | 1990-09-04 | 1997-03-18 | The Salk Institute | Production of insulin-like growth factor-1 in methylotrophic yeast cells |
| EP0511393B1 (de) | 1990-11-08 | 1996-07-31 | Japan Energy Corporation | Hirudinmutante, deren herstellung, antikoagulans, sekretorischer vektor, durch besagten vektor transformierter mikroorganismus und herstellung eines produkts durch besagten mikroorganismus |
| EP0501914A1 (de) * | 1991-02-25 | 1992-09-02 | Ciba-Geigy Ag | Verbesserte Hefe-Vektorer |
| ATE220105T1 (de) * | 1991-04-01 | 2002-07-15 | Merck & Co Inc | Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung |
| HU214698B (hu) * | 1992-04-09 | 1998-06-29 | Gyógyszerkutató Intézet Kft. | Eljárás rekombináns deszulfátó-hirudin HV-1 peptidek előállítására |
| SG49859A1 (en) * | 1992-09-04 | 2002-03-19 | Ucp Gen Pharma Ag | Process for the production of protease inhibitors |
| TW282490B (de) * | 1992-12-15 | 1996-08-01 | Ciba Geigy Ag | |
| SG49862A1 (en) * | 1993-08-04 | 2002-03-19 | Ucp Gen Pharma Ag | High molecular weight desulphatohirudin |
| DE4404168A1 (de) * | 1994-02-10 | 1995-08-17 | Hoechst Ag | Hirudinderivate und Verfahren zu deren Herstellung |
| DE69616276T2 (de) | 1995-02-09 | 2002-12-12 | Aventis Pharma Deutschland Gmbh | Verfahren zur produktion von proteinen |
| CN1225550C (zh) * | 1995-03-20 | 2005-11-02 | 诺沃奇梅兹有限公司 | 表达降低水平的金属蛋白酶的宿主细胞和在蛋白质产生中使用该宿主细胞的方法 |
| DE19529997C1 (de) | 1995-08-16 | 1997-04-10 | Hoechst Ag | Verfahren zur Inaktivierung von Carboxypeptidase Y in hirudinhaltigen Kulturbrühen |
| WO1997012045A1 (en) * | 1995-09-27 | 1997-04-03 | Chiron Corporation | Method of making a protease deficient host |
| DE69625623T2 (de) | 1996-01-31 | 2003-11-06 | Sumitomo Bakelite Co. Ltd., Tokio/Tokyo | Verfahren zur Herstellung von in Epoxyharz eingekapselter Halbleitervorrichtung |
| FR2768743B1 (fr) * | 1997-09-23 | 2001-09-14 | Bio Merieux | Procede de lyse de micro-organisme |
| GB2338236B (en) | 1998-06-13 | 2003-04-09 | Aea Technology Plc | Microbiological cell processing |
| US7795205B2 (en) | 2004-04-12 | 2010-09-14 | Canyon Pharmaceuticals, Inc. | Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor |
| EP2504355B1 (de) * | 2009-11-25 | 2015-07-22 | Novo Nordisk A/S | Verfahren zur herstellung von polypeptiden |
| EP3696274A4 (de) * | 2017-10-10 | 2021-07-28 | Ajinomoto Co., Inc. | Verfahren zur herstellung von protein |
| WO2021251383A1 (ja) | 2020-06-11 | 2021-12-16 | 味の素株式会社 | タンパク質の製造法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3342139A1 (de) * | 1983-11-22 | 1985-05-30 | Ciba-Geigy Ag, Basel | Desulfatohirudine, verfahren zu ihrer herstellung und pharmazeutische mittel |
| JPS62224284A (ja) * | 1986-03-25 | 1987-10-02 | Agency Of Ind Science & Technol | 微生物 |
| GB8809860D0 (en) * | 1988-04-26 | 1988-06-02 | Ciba Geigy Ag | Process for production of polypeptides |
| GB8817161D0 (en) * | 1988-07-19 | 1988-08-24 | Ciba Geigy Ag | Modified proteins |
| GB8817160D0 (en) * | 1988-07-19 | 1988-08-24 | Ciba Geigy Ag | Novel proteins |
-
1989
- 1989-04-28 AU AU33828/89A patent/AU614121B2/en not_active Expired
- 1989-05-01 DE DE68923123T patent/DE68923123T2/de not_active Expired - Lifetime
- 1989-05-01 EP EP89810335A patent/EP0341215B1/de not_active Expired - Lifetime
- 1989-05-01 ES ES89810335T patent/ES2072918T3/es not_active Expired - Lifetime
- 1989-05-01 AT AT89810335T patent/ATE124086T1/de not_active IP Right Cessation
- 1989-05-02 NZ NZ228955A patent/NZ228955A/en unknown
- 1989-05-02 JP JP1112294A patent/JPH0824565B2/ja not_active Expired - Lifetime
- 1989-05-02 CA CA000598488A patent/CA1340016C/en not_active Expired - Lifetime
- 1989-05-02 MX MX015887A patent/MX169508B/es unknown
- 1989-05-02 PT PT90436A patent/PT90436B/pt not_active IP Right Cessation
- 1989-05-03 FI FI892114A patent/FI103129B/fi not_active IP Right Cessation
- 1989-05-03 DK DK198902175A patent/DK175701B1/da not_active IP Right Cessation
- 1989-05-03 NO NO891837A patent/NO180593C/no not_active IP Right Cessation
- 1989-05-03 HU HU077/89A patent/HU218731B/hu unknown
- 1989-05-03 IL IL9017089A patent/IL90170A/en active IP Right Grant
- 1989-05-03 IE IE145189A patent/IE66044B1/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| NO891837L (no) | 1989-11-06 |
| NO180593B (no) | 1997-02-03 |
| NZ228955A (en) | 1991-06-25 |
| HU218731B (hu) | 2000-11-28 |
| DK175701B1 (da) | 2005-01-24 |
| MX169508B (es) | 1993-07-08 |
| IE66044B1 (en) | 1995-12-13 |
| JPH0824565B2 (ja) | 1996-03-13 |
| ATE124086T1 (de) | 1995-07-15 |
| JPH02104279A (ja) | 1990-04-17 |
| DK217589A (da) | 1989-11-05 |
| FI892114L (fi) | 1989-11-05 |
| PT90436B (pt) | 1994-11-30 |
| DE68923123D1 (de) | 1995-07-27 |
| IE891451L (en) | 1989-11-04 |
| FI103129B1 (fi) | 1999-04-30 |
| DE68923123T2 (de) | 1995-11-30 |
| EP0341215B1 (de) | 1995-06-21 |
| NO180593C (no) | 1997-05-14 |
| EP0341215A3 (de) | 1992-01-15 |
| NO891837D0 (no) | 1989-05-03 |
| DK217589D0 (da) | 1989-05-03 |
| EP0341215A2 (de) | 1989-11-08 |
| HUT54210A (en) | 1991-01-28 |
| FI103129B (fi) | 1999-04-30 |
| PT90436A (pt) | 1989-11-30 |
| CA1340016C (en) | 1998-08-25 |
| ES2072918T3 (es) | 1995-08-01 |
| IL90170A0 (en) | 1989-12-15 |
| FI892114A0 (fi) | 1989-05-03 |
| AU3382889A (en) | 1989-11-09 |
| AU614121B2 (en) | 1991-08-22 |
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