DE69535744D1 - Mutierte luziferasen - Google Patents

Mutierte luziferasen

Info

Publication number
DE69535744D1
DE69535744D1 DE69535744T DE69535744T DE69535744D1 DE 69535744 D1 DE69535744 D1 DE 69535744D1 DE 69535744 T DE69535744 T DE 69535744T DE 69535744 T DE69535744 T DE 69535744T DE 69535744 D1 DE69535744 D1 DE 69535744D1
Authority
DE
Germany
Prior art keywords
luciferases
dnas
wavelength
peak intensity
differs
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69535744T
Other languages
English (en)
Other versions
DE69535744T2 (de
Inventor
Keith V Wood
Monika G Gruber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Application granted granted Critical
Publication of DE69535744D1 publication Critical patent/DE69535744D1/de
Publication of DE69535744T2 publication Critical patent/DE69535744T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Luminescent Compositions (AREA)
DE69535744T 1994-01-03 1995-01-03 Mutierte luziferasen Expired - Lifetime DE69535744T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US17708194A 1994-01-03 1994-01-03
US177081 1994-01-03
PCT/US1995/000108 WO1995018853A1 (en) 1994-01-03 1995-01-03 Mutant luciferases

Publications (2)

Publication Number Publication Date
DE69535744D1 true DE69535744D1 (de) 2008-05-21
DE69535744T2 DE69535744T2 (de) 2009-07-02

Family

ID=22647117

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69535744T Expired - Lifetime DE69535744T2 (de) 1994-01-03 1995-01-03 Mutierte luziferasen

Country Status (9)

Country Link
US (2) US6552179B1 (de)
EP (3) EP1978091A1 (de)
JP (2) JPH08510387A (de)
AT (1) ATE391769T1 (de)
AU (1) AU698424C (de)
CA (1) CA2157476C (de)
DE (1) DE69535744T2 (de)
ES (1) ES2301160T3 (de)
WO (1) WO1995018853A1 (de)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996022376A1 (en) * 1995-01-20 1996-07-25 The Secretary Of State For Defence Mutant luciferases
GB2311525B (en) * 1995-01-20 1998-11-11 Secr Defence Mutant Luciferases
GB9707486D0 (en) * 1997-04-11 1997-05-28 Secr Defence Enzyme assays
US6602677B1 (en) 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
EP1925320A3 (de) 1998-03-27 2008-09-03 Prolume, Ltd. Luciferasen, fluoreszierende Proteine, Nukleinsäuren zur Kodierung der Luciferasen und der fluoreszierenden Proteine sowie deren Verwendung zur Diagnose, zum Screening mit hohem Durchsatz und zur Behandlung neuartiger Probleme
DE69938293T2 (de) * 1998-03-27 2009-03-12 Bruce J. Beverly Hills Bryan Luciferase, gfp fluoreszenzproteine, kodierende nukleinsaüre und ihre verwendung in der diagnose
GB9823468D0 (en) 1998-10-28 1998-12-23 Secr Defence Novel enzyme
JP3694181B2 (ja) * 1999-01-05 2005-09-14 キッコーマン株式会社 変異型ルシフェラーゼ、変異型ルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び変異型ルシフェラーゼの製造法
GB9925161D0 (en) * 1999-10-26 1999-12-22 Secr Defence Novel enzyme
AU772485C (en) * 1999-10-26 2004-11-18 Promega Corporation Novel enzyme
US7879540B1 (en) * 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
AU2003267309A1 (en) 2000-11-16 2004-04-08 Microspherix Llc Flexible and/or elastic brachytherapy seed or strand
GB0030727D0 (en) 2000-12-15 2001-01-31 Lumitech Uk Ltd Methods and kits for detecting kinase activity
GB2400659B8 (en) 2003-04-17 2007-07-09 Cambrex Bio Science Nottingham Assay methods and materials
EP2308978B8 (de) 2003-10-10 2015-04-08 Promega Corporation Luciferase biosensor
US7728118B2 (en) 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
US8227572B2 (en) * 2005-08-19 2012-07-24 Commonwealth Scientific And Industrial Research Organisation Arachnocampa luciferases
GB0517977D0 (en) * 2005-09-02 2005-10-12 Birkeland Innovasjon As Transgenic animal model for identifying modulators of protein kinase
EP2004813B1 (de) 2006-04-03 2015-06-03 Promega Corporation Permutierte und nichtpermutierte Luciferase-biosensoren, die zyklische Nukleotiden binden
WO2008109463A2 (en) * 2007-03-02 2008-09-12 University Of Massachusetts Red-shifted luciferase
WO2008109459A1 (en) * 2007-03-02 2008-09-12 University Of Massachusetts Luciferins
JP2008222084A (ja) * 2007-03-14 2008-09-25 Yamaha Motor Electronics Co Ltd 電動ゴルフカーのブレーキ劣化検出方法及びこれを用いた電動ゴルフカー
JP2008289475A (ja) * 2007-04-27 2008-12-04 National Institute Of Advanced Industrial & Technology 最大発光波長がシフトした変異型ルシフェラーゼ
ATE542903T1 (de) 2008-05-19 2012-02-15 Promega Corp Luciferase-biosensoren für camp
JP2010081908A (ja) * 2008-10-02 2010-04-15 Hitachi Ltd 変異型のホタルルシフェラーゼ
DK2990478T3 (en) * 2009-05-01 2017-05-15 Promega Corp SYNTHETIC OPLOPHORUS LUCIFERASES WITH IMPROVED LIGHT EMISSION
WO2011143339A1 (en) 2010-05-11 2011-11-17 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
US9290794B2 (en) 2010-05-11 2016-03-22 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
WO2012061530A2 (en) 2010-11-02 2012-05-10 Promega Corporation Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
US9487520B2 (en) 2010-11-02 2016-11-08 Promega Corporation Coelenterazine derivatives and methods of using same
JP6588917B2 (ja) 2014-01-29 2019-10-09 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 生細胞への適用のためのプロ基質
JP6703484B2 (ja) 2014-01-29 2020-06-03 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 細胞による取り込み測定のための、標識用試薬としての、キノンでマスクされたプローブ
EP3191600A1 (de) 2014-09-11 2017-07-19 Promega Corporation Luciferasesequenzen unter verwendung von infrarotemittierenden substraten zur herstellung von verstärkter lumineszenz
WO2016051517A1 (ja) 2014-09-30 2016-04-07 オリンパス株式会社 オレンジ色の発光を示すルシフェラーゼ
US11261478B2 (en) 2016-10-13 2022-03-01 Promega Corporation Thermal stable luciferase with improved resistance to inhibition by luciferin break-down products
JP6349003B1 (ja) 2017-03-17 2018-06-27 東洋ビーネット株式会社 耐熱性ルシフェラーゼ
JP2021526800A (ja) 2018-06-05 2021-10-11 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 熱安定性ルシフェリンと共に生物発光アッセイに使用するためのルシフェラーゼ酵素

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4968613A (en) 1987-07-29 1990-11-06 Kikkoman Corporation Luciferase gene and novel recombinant DNA as well as a method of producing luciferase
US5219737A (en) 1990-03-27 1993-06-15 Kikkoman Corporation Mutant luciferase of a firefly, mutant luciferase genes, recombinant dnas containing the genes and a method of producing mutant luciferase
US5229285A (en) 1991-06-27 1993-07-20 Kikkoman Corporation Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly

Also Published As

Publication number Publication date
DE69535744T2 (de) 2009-07-02
AU1830395A (en) 1995-08-01
AU698424B2 (en) 1998-10-29
CA2157476C (en) 2009-08-25
EP0689587A1 (de) 1996-01-03
EP1978092A1 (de) 2008-10-08
EP1978091A1 (de) 2008-10-08
EP0689587B1 (de) 2008-04-09
ES2301160T3 (es) 2008-06-16
JPH08510387A (ja) 1996-11-05
US20030166905A1 (en) 2003-09-04
JP2009039129A (ja) 2009-02-26
JP5090304B2 (ja) 2012-12-05
WO1995018853A1 (en) 1995-07-13
AU698424C (en) 2002-10-10
US6552179B1 (en) 2003-04-22
CA2157476A1 (en) 1995-07-13
ATE391769T1 (de) 2008-04-15
EP0689587A4 (de) 2001-08-08

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: KUDLEK & GRUNERT PATENTANWAELTE PARTNERSCHAFT, 803