ATE418602T1 - Mutierte luciferase mit verbesserter thermostabilität - Google Patents
Mutierte luciferase mit verbesserter thermostabilitätInfo
- Publication number
- ATE418602T1 ATE418602T1 AT04000812T AT04000812T ATE418602T1 AT E418602 T1 ATE418602 T1 AT E418602T1 AT 04000812 T AT04000812 T AT 04000812T AT 04000812 T AT04000812 T AT 04000812T AT E418602 T1 ATE418602 T1 AT E418602T1
- Authority
- AT
- Austria
- Prior art keywords
- amino acid
- acid residue
- luciferase
- photinus pyralis
- residue corresponding
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Luminescent Compositions (AREA)
- Lubricants (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB9823468.5A GB9823468D0 (en) | 1998-10-28 | 1998-10-28 | Novel enzyme |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE418602T1 true ATE418602T1 (de) | 2009-01-15 |
Family
ID=10841350
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT04000812T ATE418602T1 (de) | 1998-10-28 | 1999-10-26 | Mutierte luciferase mit verbesserter thermostabilität |
AT99950990T ATE419335T1 (de) | 1998-10-28 | 1999-10-26 | Mutierte luciferase mit verbesserter thermostabilität |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT99950990T ATE419335T1 (de) | 1998-10-28 | 1999-10-26 | Mutierte luciferase mit verbesserter thermostabilität |
Country Status (11)
Country | Link |
---|---|
US (4) | US7906298B1 (de) |
EP (9) | EP2368976A1 (de) |
JP (3) | JP5185479B2 (de) |
AT (2) | ATE418602T1 (de) |
AU (5) | AU770722B2 (de) |
CA (1) | CA2342300C (de) |
DE (2) | DE69940208D1 (de) |
DK (2) | DK1479763T3 (de) |
ES (2) | ES2319520T3 (de) |
GB (2) | GB9823468D0 (de) |
WO (1) | WO2000024878A2 (de) |
Families Citing this family (39)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6602677B1 (en) | 1997-09-19 | 2003-08-05 | Promega Corporation | Thermostable luciferases and methods of production |
GB9823468D0 (en) | 1998-10-28 | 1998-12-23 | Secr Defence | Novel enzyme |
GB9925161D0 (en) | 1999-10-26 | 1999-12-22 | Secr Defence | Novel enzyme |
ATE374820T1 (de) * | 1999-10-26 | 2007-10-15 | Secr Defence | Mutantenluciferase |
AU2003272174A1 (en) * | 2002-10-21 | 2004-05-04 | Biotage Ab | Use of osmolytes to heat stabilise luciferase and/or apyrase |
GB2400659B8 (en) | 2003-04-17 | 2007-07-09 | Cambrex Bio Science Nottingham | Assay methods and materials |
GB0516455D0 (en) * | 2005-08-10 | 2005-09-14 | Lumora Ltd | pH tolerant luciferase |
NZ565938A (en) | 2005-08-19 | 2011-10-28 | Commw Scient Ind Res Org | Arachnocampa (Diptera) ATP-dependent luciferases |
JP4999341B2 (ja) * | 2005-09-06 | 2012-08-15 | 株式会社バイオエネックス | 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法 |
US7807429B2 (en) | 2006-02-13 | 2010-10-05 | Connecticut College | Isolated luciferase gene of L. italica |
WO2007120522A2 (en) | 2006-04-03 | 2007-10-25 | Promega Corporation | Permuted and nonpermuted luciferase biosensors |
JP5188771B2 (ja) | 2007-09-26 | 2013-04-24 | 株式会社日立製作所 | 変異型ホタルルシフェラーゼ |
US20090286299A1 (en) * | 2008-05-15 | 2009-11-19 | Mostafa Ronaghi | Engineered luciferases |
JP6110591B2 (ja) | 2008-05-19 | 2017-04-05 | プロメガ コーポレイションPromega Corporation | cAMPのためのルシフェラーゼバイオセンサー |
CN102159948B (zh) | 2008-07-22 | 2014-06-25 | 普罗美加公司 | 基于adp检测的发光磷酸转移酶或atp水解酶测定 |
US20100020170A1 (en) | 2008-07-24 | 2010-01-28 | Higgins-Luthman Michael J | Vehicle Imaging System |
JP2011120559A (ja) | 2008-12-26 | 2011-06-23 | Kikkoman Corp | ホタルルシフェラーゼ、その遺伝子、およびホタルルシフェラーゼの製造法 |
CN102439444B (zh) | 2009-01-29 | 2014-10-22 | 联邦科学技术研究组织 | 测量g蛋白偶联受体激活 |
JP5587644B2 (ja) * | 2010-03-15 | 2014-09-10 | キッコーマン株式会社 | ホタルルシフェラーゼ、その遺伝子、およびホタルルシフェラーゼの製造法 |
US8735559B2 (en) | 2010-05-11 | 2014-05-27 | Promega Corporation | Mutant protease biosensors with enhanced detection characteristics |
ES2543913T3 (es) | 2010-11-02 | 2015-08-25 | Promega Corporation | Derivados de coelenterazina y métodos de uso de los mismos |
SG10202103336SA (en) | 2010-11-02 | 2021-04-29 | Promega Corp | Novel coelenterazine substrates and methods of use |
JP6147669B2 (ja) * | 2010-12-03 | 2017-06-14 | ジーン・ストリーム・ピーティワイ・リミテッド | 改良光放射分子 |
JP5887071B2 (ja) * | 2011-06-01 | 2016-03-16 | 出光興産株式会社 | 粒状物の貯蔵装置および水添石油樹脂ペレットの貯蔵装置 |
JP6421977B2 (ja) * | 2011-11-11 | 2018-11-14 | プロメガ コーポレイションPromega Corporation | 検出特性を向上させた変異プロテアーゼバイオセンサー |
AU2013204332B2 (en) | 2012-04-16 | 2015-07-16 | Commonwealth Scientific And Industrial Research Organisation | Methods and systems for detecting an analyte or classifying a sample |
EP3693471B1 (de) | 2013-02-22 | 2024-01-03 | Promega Corporation | Stabilisierte formulierung zum lumineszenznachweis von luciferase und nukleosidphosphaten |
EP3099683B1 (de) | 2014-01-29 | 2020-08-05 | Promega Corporation | Chinonmaskierte sonden als markierungsreagenzien für zellaufnahmemessungen |
JP6588917B2 (ja) | 2014-01-29 | 2019-10-09 | プロメガ コーポレイションPromega Corporation | 生細胞への適用のためのプロ基質 |
EP3191600A1 (de) | 2014-09-11 | 2017-07-19 | Promega Corporation | Luciferasesequenzen unter verwendung von infrarotemittierenden substraten zur herstellung von verstärkter lumineszenz |
US9677117B2 (en) | 2014-10-08 | 2017-06-13 | Promega Corporation | Bioluminescent succinate detection assay |
EP3056902B1 (de) | 2015-02-16 | 2020-04-01 | Universite De Bordeaux | Neuartige spannungsabhängige Ionenkanalfusionen und Verwendungsverfahren dafür |
JP7175881B2 (ja) * | 2016-10-13 | 2022-11-21 | プロメガ コーポレイション | ルシフェリン分解産物による阻害に対して改善された耐性を持つ熱安定性ルシフェラーゼ |
PL3538665T3 (pl) | 2016-11-14 | 2024-05-06 | PPB Technology Pty Ltd | Cząsteczki czujnika proteazy |
JP6349003B1 (ja) | 2017-03-17 | 2018-06-27 | 東洋ビーネット株式会社 | 耐熱性ルシフェラーゼ |
PL3665481T3 (pl) | 2017-08-08 | 2024-02-05 | PPB Technology Pty Ltd | Czujniki węglowodanów |
KR20200071724A (ko) | 2017-10-18 | 2020-06-19 | 유니버시떼 드 몬트리얼 | G-단백질 활성화의 평가를 위한 시스템 및 방법 |
WO2021162123A1 (ja) | 2020-02-14 | 2021-08-19 | キッコーマン株式会社 | 試料中のatp、並びにamp及び/又はadpを測定するための液体組成物 |
EP3882632A1 (de) | 2020-03-18 | 2021-09-22 | Universite De Bordeaux | Neuartige ionenleitende kanalfusionuntereinheiten und verfahren zur verwendung davon |
Family Cites Families (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5782879A (en) | 1980-11-12 | 1982-05-24 | Mita Ind Co Ltd | Photosensitive drum of electrostatic copier |
US5583024A (en) | 1985-12-02 | 1996-12-10 | The Regents Of The University Of California | Recombinant expression of Coleoptera luciferase |
JPH088864B2 (ja) | 1988-04-12 | 1996-01-31 | キッコーマン株式会社 | ルシフェラーゼ |
US5049358A (en) | 1988-09-30 | 1991-09-17 | Miles Inc. | Composition and test device for assaying for proteins |
US5196524A (en) * | 1989-01-06 | 1993-03-23 | Eli Lilly And Company | Fusion reporter gene for bacterial luciferase |
US5219737A (en) | 1990-03-27 | 1993-06-15 | Kikkoman Corporation | Mutant luciferase of a firefly, mutant luciferase genes, recombinant dnas containing the genes and a method of producing mutant luciferase |
US5480789A (en) * | 1991-04-01 | 1996-01-02 | Florigene Europe B.V. | Genetically transformed rose plants and methods for their production |
JP3048466B2 (ja) | 1991-06-27 | 2000-06-05 | キッコーマン株式会社 | 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体dna、及び耐熱性ホタルルシフェラーゼの製造法 |
US5229285A (en) | 1991-06-27 | 1993-07-20 | Kikkoman Corporation | Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly |
GB9301118D0 (en) | 1993-01-21 | 1993-03-10 | Secr Defence | Enzyme linked assays |
US5610335A (en) | 1993-05-26 | 1997-03-11 | Cornell Research Foundation | Microelectromechanical lateral accelerometer |
US6387675B1 (en) | 1994-01-03 | 2002-05-14 | Promega Corporation | Mutant luciferases |
JPH08510387A (ja) | 1994-01-03 | 1996-11-05 | プロメガ・コーポレイシヨン | 突然変異体ルシフェラーゼ |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
GB9501170D0 (en) * | 1994-03-23 | 1995-03-08 | Secr Defence | Luciferases |
GB2301592B (en) | 1994-03-23 | 1998-06-17 | Secr Defence | Luciferases |
WO1996002665A1 (en) | 1994-07-13 | 1996-02-01 | The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland | Microbiological test method and reagents |
US5670356A (en) | 1994-12-12 | 1997-09-23 | Promega Corporation | Modified luciferase |
EP0804587B1 (de) | 1995-01-20 | 2004-08-18 | The Secretary of State for Defence | Mutierte luziferasen |
US5716851A (en) | 1996-01-16 | 1998-02-10 | Bayer Corporation | Glass/cellulose as protein reagent |
JPH09294600A (ja) | 1996-04-26 | 1997-11-18 | Kikkoman Corp | 複数のプロモーター活性の測定法 |
GB2315763A (en) * | 1996-07-31 | 1998-02-11 | Procter & Gamble | Preparation of an agglomerated detergent composition comprising a surfactant a an acid source |
CA2266423A1 (en) | 1996-09-27 | 1998-04-02 | Maxygen, Inc. | Methods for optimization of gene therapy by recursive sequence shuffling and selection |
GB9707486D0 (en) * | 1997-04-11 | 1997-05-28 | Secr Defence | Enzyme assays |
US6074859A (en) * | 1997-07-08 | 2000-06-13 | Kikkoman Corporation | Mutant-type bioluminescent protein, and process for producing the mutant-type bioluminescent protein |
JP4537573B2 (ja) * | 1997-09-19 | 2010-09-01 | プロメガ・コーポレイシヨン | 熱安定ルシフェラーゼと生産方法 |
US6602677B1 (en) | 1997-09-19 | 2003-08-05 | Promega Corporation | Thermostable luciferases and methods of production |
GB9823468D0 (en) | 1998-10-28 | 1998-12-23 | Secr Defence | Novel enzyme |
ATE374820T1 (de) * | 1999-10-26 | 2007-10-15 | Secr Defence | Mutantenluciferase |
US7083911B2 (en) | 2001-02-16 | 2006-08-01 | Promega Corporation | Method for detection of ATP |
WO2005035719A2 (en) | 2003-10-03 | 2005-04-21 | Board Of Control Of Michigan Technological University | Modified luciferase |
JP5244942B2 (ja) | 2011-04-25 | 2013-07-24 | 株式会社グレイプ | 筋交い取付構造 |
-
1998
- 1998-10-28 GB GBGB9823468.5A patent/GB9823468D0/en not_active Ceased
-
1999
- 1999-10-26 DE DE69940208T patent/DE69940208D1/de not_active Expired - Lifetime
- 1999-10-26 EP EP10179361A patent/EP2368976A1/de not_active Ceased
- 1999-10-26 EP EP10179359A patent/EP2366778B1/de not_active Expired - Lifetime
- 1999-10-26 EP EP08013254.1A patent/EP2071023B1/de not_active Expired - Lifetime
- 1999-10-26 EP EP99950990A patent/EP1124944B1/de not_active Expired - Lifetime
- 1999-10-26 AU AU63573/99A patent/AU770722B2/en not_active Ceased
- 1999-10-26 ES ES04000812T patent/ES2319520T3/es not_active Expired - Lifetime
- 1999-10-26 US US09/763,824 patent/US7906298B1/en not_active Expired - Fee Related
- 1999-10-26 JP JP2000578432A patent/JP5185479B2/ja not_active Expired - Lifetime
- 1999-10-26 CA CA002342300A patent/CA2342300C/en not_active Expired - Fee Related
- 1999-10-26 EP EP10179372.7A patent/EP2366779B1/de not_active Expired - Lifetime
- 1999-10-26 EP EP10179358.6A patent/EP2366777B1/de not_active Expired - Lifetime
- 1999-10-26 GB GB9925162A patent/GB2345913B/en not_active Expired - Lifetime
- 1999-10-26 DK DK04000812T patent/DK1479763T3/da active
- 1999-10-26 AT AT04000812T patent/ATE418602T1/de not_active IP Right Cessation
- 1999-10-26 DK DK99950990T patent/DK1124944T3/da active
- 1999-10-26 WO PCT/GB1999/003538 patent/WO2000024878A2/en active IP Right Grant
- 1999-10-26 AT AT99950990T patent/ATE419335T1/de not_active IP Right Cessation
- 1999-10-26 ES ES99950990T patent/ES2319941T3/es not_active Expired - Lifetime
- 1999-10-26 EP EP10179373.5A patent/EP2366780B1/de not_active Expired - Lifetime
- 1999-10-26 DE DE69940172T patent/DE69940172D1/de not_active Expired - Lifetime
- 1999-10-26 EP EP04000812A patent/EP1479763B1/de not_active Expired - Lifetime
- 1999-10-26 EP EP10179370.1A patent/EP2368977B1/de not_active Expired - Lifetime
-
2004
- 2004-01-23 AU AU2004200277A patent/AU2004200277B2/en not_active Ceased
- 2004-05-25 AU AU2004202276A patent/AU2004202276B2/en not_active Ceased
- 2004-05-25 AU AU2004202277A patent/AU2004202277B2/en not_active Ceased
-
2008
- 2008-08-01 AU AU2008203449A patent/AU2008203449A1/en not_active Abandoned
-
2011
- 2011-02-09 US US13/023,704 patent/US8652794B2/en not_active Expired - Fee Related
- 2011-02-16 JP JP2011031071A patent/JP2011120605A/ja active Pending
-
2012
- 2012-04-05 JP JP2012086569A patent/JP5951325B2/ja not_active Expired - Lifetime
-
2014
- 2014-01-17 US US14/157,951 patent/US20140170686A1/en not_active Abandoned
- 2014-01-17 US US14/157,959 patent/US20140170687A1/en not_active Abandoned
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Legal Events
Date | Code | Title | Description |
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RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |