ATE418602T1 - Mutierte luciferase mit verbesserter thermostabilität - Google Patents

Mutierte luciferase mit verbesserter thermostabilität

Info

Publication number
ATE418602T1
ATE418602T1 AT04000812T AT04000812T ATE418602T1 AT E418602 T1 ATE418602 T1 AT E418602T1 AT 04000812 T AT04000812 T AT 04000812T AT 04000812 T AT04000812 T AT 04000812T AT E418602 T1 ATE418602 T1 AT E418602T1
Authority
AT
Austria
Prior art keywords
amino acid
acid residue
luciferase
photinus pyralis
residue corresponding
Prior art date
Application number
AT04000812T
Other languages
English (en)
Inventor
David James Squirrell
Melenie Jane Murphy
Rachel Louise Leslie
Christopher Robin Lowe
Laurence Carlo Tisi
James Augustus Henry Murray
Peter John White
Original Assignee
3M Innovative Properties Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 3M Innovative Properties Co filed Critical 3M Innovative Properties Co
Application granted granted Critical
Publication of ATE418602T1 publication Critical patent/ATE418602T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Luminescent Compositions (AREA)
  • Lubricants (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT04000812T 1998-10-28 1999-10-26 Mutierte luciferase mit verbesserter thermostabilität ATE418602T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB9823468.5A GB9823468D0 (en) 1998-10-28 1998-10-28 Novel enzyme

Publications (1)

Publication Number Publication Date
ATE418602T1 true ATE418602T1 (de) 2009-01-15

Family

ID=10841350

Family Applications (2)

Application Number Title Priority Date Filing Date
AT04000812T ATE418602T1 (de) 1998-10-28 1999-10-26 Mutierte luciferase mit verbesserter thermostabilität
AT99950990T ATE419335T1 (de) 1998-10-28 1999-10-26 Mutierte luciferase mit verbesserter thermostabilität

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT99950990T ATE419335T1 (de) 1998-10-28 1999-10-26 Mutierte luciferase mit verbesserter thermostabilität

Country Status (11)

Country Link
US (4) US7906298B1 (de)
EP (9) EP2368976A1 (de)
JP (3) JP5185479B2 (de)
AT (2) ATE418602T1 (de)
AU (5) AU770722B2 (de)
CA (1) CA2342300C (de)
DE (2) DE69940208D1 (de)
DK (2) DK1479763T3 (de)
ES (2) ES2319520T3 (de)
GB (2) GB9823468D0 (de)
WO (1) WO2000024878A2 (de)

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JP5188771B2 (ja) 2007-09-26 2013-04-24 株式会社日立製作所 変異型ホタルルシフェラーゼ
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JP6110591B2 (ja) 2008-05-19 2017-04-05 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ ｃＡＭＰのためのルシフェラーゼバイオセンサー
CN102159948B (zh) 2008-07-22 2014-06-25 普罗美加公司 基于adp检测的发光磷酸转移酶或atp水解酶测定
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US8735559B2 (en) 2010-05-11 2014-05-27 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
ES2543913T3 (es) 2010-11-02 2015-08-25 Promega Corporation Derivados de coelenterazina y métodos de uso de los mismos
SG10202103336SA (en) 2010-11-02 2021-04-29 Promega Corp Novel coelenterazine substrates and methods of use
JP6147669B2 (ja) * 2010-12-03 2017-06-14 ジーン・ストリーム・ピーティワイ・リミテッド 改良光放射分子
JP5887071B2 (ja) * 2011-06-01 2016-03-16 出光興産株式会社 粒状物の貯蔵装置および水添石油樹脂ペレットの貯蔵装置
JP6421977B2 (ja) * 2011-11-11 2018-11-14 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 検出特性を向上させた変異プロテアーゼバイオセンサー
AU2013204332B2 (en) 2012-04-16 2015-07-16 Commonwealth Scientific And Industrial Research Organisation Methods and systems for detecting an analyte or classifying a sample
EP3693471B1 (de) 2013-02-22 2024-01-03 Promega Corporation Stabilisierte formulierung zum lumineszenznachweis von luciferase und nukleosidphosphaten
EP3099683B1 (de) 2014-01-29 2020-08-05 Promega Corporation Chinonmaskierte sonden als markierungsreagenzien für zellaufnahmemessungen
JP6588917B2 (ja) 2014-01-29 2019-10-09 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 生細胞への適用のためのプロ基質
EP3191600A1 (de) 2014-09-11 2017-07-19 Promega Corporation Luciferasesequenzen unter verwendung von infrarotemittierenden substraten zur herstellung von verstärkter lumineszenz
US9677117B2 (en) 2014-10-08 2017-06-13 Promega Corporation Bioluminescent succinate detection assay
EP3056902B1 (de) 2015-02-16 2020-04-01 Universite De Bordeaux Neuartige spannungsabhängige Ionenkanalfusionen und Verwendungsverfahren dafür
JP7175881B2 (ja) * 2016-10-13 2022-11-21 プロメガ コーポレイション ルシフェリン分解産物による阻害に対して改善された耐性を持つ熱安定性ルシフェラーゼ
PL3538665T3 (pl) 2016-11-14 2024-05-06 PPB Technology Pty Ltd Cząsteczki czujnika proteazy
JP6349003B1 (ja) 2017-03-17 2018-06-27 東洋ビーネット株式会社 耐熱性ルシフェラーゼ
PL3665481T3 (pl) 2017-08-08 2024-02-05 PPB Technology Pty Ltd Czujniki węglowodanów
KR20200071724A (ko) 2017-10-18 2020-06-19 유니버시떼 드 몬트리얼 G-단백질 활성화의 평가를 위한 시스템 및 방법
WO2021162123A1 (ja) 2020-02-14 2021-08-19 キッコーマン株式会社 試料中のａｔｐ、並びにａｍｐ及び／又はａｄｐを測定するための液体組成物
EP3882632A1 (de) 2020-03-18 2021-09-22 Universite De Bordeaux Neuartige ionenleitende kanalfusionuntereinheiten und verfahren zur verwendung davon

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Also Published As

Publication number Publication date
US20140170687A1 (en) 2014-06-19
CA2342300A1 (en) 2000-05-04
EP2071023B1 (de) 2016-04-27
DE69940208D1 (de) 2009-02-12
EP2366777B1 (de) 2018-07-25
WO2000024878A2 (en) 2000-05-04
US20110177540A1 (en) 2011-07-21
JP5185479B2 (ja) 2013-04-17
EP2071023A2 (de) 2009-06-17
EP2366778B1 (de) 2012-08-22
US20140170686A1 (en) 2014-06-19
AU2004200277B2 (en) 2005-11-24
EP2071023A3 (de) 2009-10-14
US8652794B2 (en) 2014-02-18
GB9823468D0 (en) 1998-12-23
EP1479763A2 (de) 2004-11-24
EP2366777A1 (de) 2011-09-21
EP1479763B1 (de) 2008-12-24
EP1479763A3 (de) 2005-05-25
DK1124944T3 (da) 2009-04-14
EP2368976A1 (de) 2011-09-28
EP2366779A1 (de) 2011-09-21
ES2319941T3 (es) 2009-05-14
JP2011120605A (ja) 2011-06-23
GB9925162D0 (en) 1999-12-22
AU2008203449A1 (en) 2008-08-28
DE69940172D1 (de) 2009-02-05
ATE419335T1 (de) 2009-01-15
AU2004202277B2 (en) 2008-05-01
JP2012161325A (ja) 2012-08-30
EP1124944A2 (de) 2001-08-22
EP2368977B1 (de) 2018-06-27
EP2366780A1 (de) 2011-09-21
GB2345913B (en) 2004-03-17
EP2368977A1 (de) 2011-09-28
WO2000024878A3 (en) 2000-08-03
AU2004202276A1 (en) 2004-06-17
JP2003518912A (ja) 2003-06-17
GB2345913A (en) 2000-07-26
DK1479763T3 (da) 2009-04-06
EP2071023A8 (de) 2010-06-02
AU6357399A (en) 2000-05-15
US7906298B1 (en) 2011-03-15
EP2366780B1 (de) 2018-06-13
AU2004200277A1 (en) 2004-02-19
ES2319520T3 (es) 2009-05-08
EP2366779B1 (de) 2018-07-11
AU2004202277A1 (en) 2004-06-17
AU770722B2 (en) 2004-02-26
JP5951325B2 (ja) 2016-07-13
CA2342300C (en) 2009-08-11
EP1124944B1 (de) 2008-12-31
AU2004202276B2 (en) 2008-05-01
EP2366778A1 (de) 2011-09-21

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