GB2301592A - Luciferases - Google Patents

Luciferases

Info

Publication number
GB2301592A
GB2301592A GB9619832A GB9619832A GB2301592A GB 2301592 A GB2301592 A GB 2301592A GB 9619832 A GB9619832 A GB 9619832A GB 9619832 A GB9619832 A GB 9619832A GB 2301592 A GB2301592 A GB 2301592A
Authority
GB
United Kingdom
Prior art keywords
proteins
luciferases
luciola
amino acid
photinus pyralis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB9619832A
Other versions
GB2301592A8 (en
GB2301592B (en
GB9619832D0 (en
Inventor
Christopher Robin Lowe
Peter John White
James Augustus Henry Murray
David James Squirrel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UK Secretary of State for Defence
Original Assignee
UK Secretary of State for Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB9405750A external-priority patent/GB9405750D0/en
Priority claimed from GBGB9501170.6A external-priority patent/GB9501170D0/en
Application filed by UK Secretary of State for Defence filed Critical UK Secretary of State for Defence
Priority to GB9619832A priority Critical patent/GB2301592B/en
Publication of GB9619832D0 publication Critical patent/GB9619832D0/en
Publication of GB2301592A publication Critical patent/GB2301592A/en
Publication of GB2301592A8 publication Critical patent/GB2301592A8/en
Application granted granted Critical
Publication of GB2301592B publication Critical patent/GB2301592B/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Proteins are provided having luciferase activity with greater heat stability than wildtype luciferases by replacing the glutamate equivalent to that at position 354 of Photinus pyralis luciferase or 356 of Luciola luciferases with an alternative amino acid, particularly lysine. DNA, vectors and cells that encode for and express the proteins are also provided as are test kits and reagents for carrying out luminescence assays using the proteins of the invention. Preferred proteins have a second replaced amino acid at a position equivalent to position 215 of Photinus pyralis luciferase or 217 of Luciola luciferases.
GB9619832A 1994-03-23 1995-03-22 Luciferases Expired - Lifetime GB2301592B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB9619832A GB2301592B (en) 1994-03-23 1995-03-22 Luciferases

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB9405750A GB9405750D0 (en) 1994-03-23 1994-03-23 Luciferases
GBGB9501170.6A GB9501170D0 (en) 1994-03-23 1995-01-20 Luciferases
PCT/GB1995/000629 WO1995025798A1 (en) 1994-03-23 1995-03-22 Luciferases
GB9619832A GB2301592B (en) 1994-03-23 1995-03-22 Luciferases

Publications (4)

Publication Number Publication Date
GB9619832D0 GB9619832D0 (en) 1996-11-06
GB2301592A true GB2301592A (en) 1996-12-11
GB2301592A8 GB2301592A8 (en) 1997-01-06
GB2301592B GB2301592B (en) 1998-06-17

Family

ID=27267115

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9619832A Expired - Lifetime GB2301592B (en) 1994-03-23 1995-03-22 Luciferases

Country Status (1)

Country Link
GB (1) GB2301592B (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7906298B1 (en) 1998-10-28 2011-03-15 Promega Corporation Thermostable Photinus pyralis luciferase mutant
US8030017B2 (en) 1997-09-19 2011-10-04 Promega Corporation Thermostable luciferases and methods of production
US8669087B1 (en) 1999-10-26 2014-03-11 Promega Corporation Luciferase mutant
US9732373B2 (en) 2014-09-11 2017-08-15 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0524448A1 (en) * 1991-06-27 1993-01-27 Kikkoman Corporation Thermostable luciferase of a firefly, gene of a thermostable luciferase of a firefly, novel recombinant DNA, and process for the preparation of a thermostable luciferase of a firefly

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0524448A1 (en) * 1991-06-27 1993-01-27 Kikkoman Corporation Thermostable luciferase of a firefly, gene of a thermostable luciferase of a firefly, novel recombinant DNA, and process for the preparation of a thermostable luciferase of a firefly

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
EMBL Database, Accession No.X65316, Identification CVPGEMLUC, Promega Cloning Vector pGEM-luc *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8030017B2 (en) 1997-09-19 2011-10-04 Promega Corporation Thermostable luciferases and methods of production
US8822170B2 (en) 1997-09-19 2014-09-02 Promega Corporation Thermostable luciferases and methods of production
US7906298B1 (en) 1998-10-28 2011-03-15 Promega Corporation Thermostable Photinus pyralis luciferase mutant
US8652794B2 (en) 1998-10-28 2014-02-18 Promega Corporation Mutant luciferase
US8669087B1 (en) 1999-10-26 2014-03-11 Promega Corporation Luciferase mutant
US9732373B2 (en) 2014-09-11 2017-08-15 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
US10550420B2 (en) 2014-09-11 2020-02-04 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
US11293047B2 (en) 2014-09-11 2022-04-05 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence

Also Published As

Publication number Publication date
GB2301592A8 (en) 1997-01-06
GB2301592B (en) 1998-06-17
GB9619832D0 (en) 1996-11-06

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Legal Events

Date Code Title Description
711B Application made for correction of error (sect. 117/77)
711L Appl. made for correction of error (sect. 117/77) now open to opposition
711G Correction allowed (sect. 117/1977)
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)

Free format text: REGISTERED BETWEEN 20090924 AND 20090930

PE20 Patent expired after termination of 20 years

Expiry date: 20150321