JPS6451086A - Luciferase gene - Google Patents

Luciferase gene

Info

Publication number
JPS6451086A
JPS6451086A JP20519487A JP20519487A JPS6451086A JP S6451086 A JPS6451086 A JP S6451086A JP 20519487 A JP20519487 A JP 20519487A JP 20519487 A JP20519487 A JP 20519487A JP S6451086 A JPS6451086 A JP S6451086A
Authority
JP
Japan
Prior art keywords
dna
luciferase
gene
rna
prepared
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP20519487A
Other languages
Japanese (ja)
Other versions
JPH0612995B2 (en
Inventor
Tsutomu Masuda
Hiroki Tatsumi
Eiichi Nakano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kikkoman Corp
Original Assignee
Kikkoman Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kikkoman Corp filed Critical Kikkoman Corp
Priority to JP20519487A priority Critical patent/JPH0612995B2/en
Priority to US07/224,445 priority patent/US4968613A/en
Priority to DE3887223T priority patent/DE3887223T2/en
Priority to EP88112233A priority patent/EP0301541B1/en
Publication of JPS6451086A publication Critical patent/JPS6451086A/en
Publication of JPH0612995B2 publication Critical patent/JPH0612995B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE:To obtain a luciferase gene originated from Luciola cruciata and useful for the quick and efficient production of luciferase useful as an enzyme for the determination of ATP, by using gene recombination technique. CONSTITUTION:m-RNA is prepared from the tail of Luciola cruciata and a c-DNA is synthesized from the m-RNA. The c-DNA is integrated into a vector DNA (e.g. plasmid pUC) to obtain various recombinant DNAs. Various recombinant strains are prepared by transforming E.coli, etc., with said DNAs. Microbial cell containing said recombinant is selected from the strains by a colony hybridization method using a probe consisting of a <32>P-labeled DNA containing a gene coding luciferase originated from Photinus pyraris. The plasmid DNA is digested with a restriction enzyme to obtain a DNA containing a c-DNA coding luciferase.
JP20519487A 1987-07-29 1987-08-20 Luciferase gene Expired - Lifetime JPH0612995B2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP20519487A JPH0612995B2 (en) 1987-08-20 1987-08-20 Luciferase gene
US07/224,445 US4968613A (en) 1987-07-29 1988-07-26 Luciferase gene and novel recombinant DNA as well as a method of producing luciferase
DE3887223T DE3887223T2 (en) 1987-07-29 1988-07-28 Luciferase gene and new recombinant DNA and a process for the production of luciferase.
EP88112233A EP0301541B1 (en) 1987-07-29 1988-07-28 Luciferase gene and novel recombinant DNA as well as a method of producing luciferase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP20519487A JPH0612995B2 (en) 1987-08-20 1987-08-20 Luciferase gene

Publications (2)

Publication Number Publication Date
JPS6451086A true JPS6451086A (en) 1989-02-27
JPH0612995B2 JPH0612995B2 (en) 1994-02-23

Family

ID=16502962

Family Applications (1)

Application Number Title Priority Date Filing Date
JP20519487A Expired - Lifetime JPH0612995B2 (en) 1987-07-29 1987-08-20 Luciferase gene

Country Status (1)

Country Link
JP (1) JPH0612995B2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06303982A (en) * 1993-04-21 1994-11-01 Chisso Corp Firefly enzyme luciferase gene
WO2011058767A1 (en) 2009-11-10 2011-05-19 キッコーマン株式会社 Firefly luciferase and gene thereof, and process for production of firefly luciferase
WO2021162123A1 (en) 2020-02-14 2021-08-19 キッコーマン株式会社 Liquid composition for measuring atp, and amp and/or adp in samples

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06303982A (en) * 1993-04-21 1994-11-01 Chisso Corp Firefly enzyme luciferase gene
WO2011058767A1 (en) 2009-11-10 2011-05-19 キッコーマン株式会社 Firefly luciferase and gene thereof, and process for production of firefly luciferase
WO2021162123A1 (en) 2020-02-14 2021-08-19 キッコーマン株式会社 Liquid composition for measuring atp, and amp and/or adp in samples

Also Published As

Publication number Publication date
JPH0612995B2 (en) 1994-02-23

Similar Documents

Publication Publication Date Title
EP1281762B1 (en) Luciferases
DE69633171T2 (en) MUTATED LUCIFERASES
US6265177B1 (en) Enzyme assay for mutant firefly luciferase
Engebrecht et al. Bacterial bioluminescence: isolation and genetic analysis of functions from Vibrio fischeri
JP5185479B2 (en) New enzyme
CA2105984A1 (en) Cloning and expression of renilla luciferase
CA2045175A1 (en) Production of proteins using homologous recombination
EP0689587A4 (en) Mutant luciferases
JP2003512071A (en) Mutant luciferase
Aliabadi et al. Oxygen-regulated stimulons of Salmonella typhimurium identified by Mu d (Ap lac) operon fusions
JPS6451086A (en) Luciferase gene
Shiiba et al. Isolation of giant mitochondrial nucleoids from the yeast Saccharomyces cerevisiae
Okabe et al. Hydroxylamine stimulates carboxylase activity and inhibits oxygenase activity of cyanobacterial RuBP carboxylase/oxygenase
JPS6486879A (en) Dna fragment
Merril et al. Disco-ordinate expression of the Escherichia coli gal operon after prophage lambda induction
EP0263716A3 (en) A process for the preparation of 5&#39;-guanylic acid and expression vectors for use therein
JPS6434289A (en) Novel recombinant dna
Lorenz Isolation and expression of acDNA encoding a luciferase from Renilla reniformis.
EP1707621A2 (en) Mutant bioluminescent protein, and process for producing the mutant bioluminescent protein
IT1272998B (en) Method for the in vitro amplification and expression of exogenous nucleic acid sequences in mammal cells
MXPA96004194A (en) Lucifera