JPS6486879A - Dna fragment - Google Patents

Dna fragment

Info

Publication number
JPS6486879A
JPS6486879A JP24695087A JP24695087A JPS6486879A JP S6486879 A JPS6486879 A JP S6486879A JP 24695087 A JP24695087 A JP 24695087A JP 24695087 A JP24695087 A JP 24695087A JP S6486879 A JPS6486879 A JP S6486879A
Authority
JP
Japan
Prior art keywords
cdna
catalase
restriction enzyme
yeast
candida
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP24695087A
Other languages
Japanese (ja)
Inventor
Atsuo Tanaka
Atsumi Ueda
Tatsuyuki Kamiryo
Yutaka Teranishi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Kasei Corp
Original Assignee
Mitsubishi Kasei Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Kasei Corp filed Critical Mitsubishi Kasei Corp
Priority to JP24695087A priority Critical patent/JPS6486879A/en
Publication of JPS6486879A publication Critical patent/JPS6486879A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PURPOSE:To obtain a DNA fragment containing catalase gene of fungus specifically induced in cultivation with n-alkane. CONSTITUTION:Total RNA is separated from a yeast belonging to the genus Candida, e.g. yeast cell Candida tropicalis grown in n-alkane and purified by applying oligo(dT)cellulose column chromatography to liberate poly(A)-containing RNA (mRNA). Corresponding cDNA library is obtained from the above- mentioned mRNA and the cDNA is methylated by DNA methylase to protect restriction enzyme cutting site and then DNA linker containing the above- mentioned restriction enzyme cutting site at the both end is added thereto and the added cDNA is digested by the restriction enzyme. The aimed cDNA is obtained by cloning the digested cDNA to cloning vector, infecting into Escherichia coli, cultivating in a culture media containing IPTG, etc. and screening formed plaque using an antibody which is specific to catalase of Candida yeast. Promoter of fungus catalase is useful in expressing an extraneous gene using a non-ethanol-fermentable carbon source.
JP24695087A 1987-09-30 1987-09-30 Dna fragment Pending JPS6486879A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP24695087A JPS6486879A (en) 1987-09-30 1987-09-30 Dna fragment

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP24695087A JPS6486879A (en) 1987-09-30 1987-09-30 Dna fragment

Publications (1)

Publication Number Publication Date
JPS6486879A true JPS6486879A (en) 1989-03-31

Family

ID=17156148

Family Applications (1)

Application Number Title Priority Date Filing Date
JP24695087A Pending JPS6486879A (en) 1987-09-30 1987-09-30 Dna fragment

Country Status (1)

Country Link
JP (1) JPS6486879A (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995001443A1 (en) * 1993-07-01 1995-01-12 E.I. Du Pont De Nemours And Company Microbial transformants of hansenula that express glycolate oxidase and catalase
WO1995001444A1 (en) * 1993-07-01 1995-01-12 E.I. Du Pont De Nemours And Company Glycolate oxidase production
WO2001046431A1 (en) * 1999-12-21 2001-06-28 Fudan University A novel polypeptide, hydrogen peroxidase 10 and the polynucleotide encoding the polypeptide
CN1089111C (en) * 1997-12-11 2002-08-14 财团法人生物技术开发中心 New catalase, its gene and composite containing it, and its preparation method
WO2008151999A1 (en) 2007-06-11 2008-12-18 Novozymes A/S A process for combined biopolishing and bleach clean-up
WO2014086659A2 (en) 2012-12-06 2014-06-12 Ahmedabad Textile Industry's Research Association Method for enzymatical preparation of textiles
WO2014143773A1 (en) 2013-03-15 2014-09-18 Lubrizol Advanced Materials, Inc. Itaconic acid polymers

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BIOCHIM BIOPHYS ACTA=1987 *
EUR J BIOCHEM=1986 *
FEBS LETT=1987 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995001443A1 (en) * 1993-07-01 1995-01-12 E.I. Du Pont De Nemours And Company Microbial transformants of hansenula that express glycolate oxidase and catalase
WO1995001444A1 (en) * 1993-07-01 1995-01-12 E.I. Du Pont De Nemours And Company Glycolate oxidase production
CN1089111C (en) * 1997-12-11 2002-08-14 财团法人生物技术开发中心 New catalase, its gene and composite containing it, and its preparation method
WO2001046431A1 (en) * 1999-12-21 2001-06-28 Fudan University A novel polypeptide, hydrogen peroxidase 10 and the polynucleotide encoding the polypeptide
WO2008151999A1 (en) 2007-06-11 2008-12-18 Novozymes A/S A process for combined biopolishing and bleach clean-up
WO2014086659A2 (en) 2012-12-06 2014-06-12 Ahmedabad Textile Industry's Research Association Method for enzymatical preparation of textiles
WO2014143773A1 (en) 2013-03-15 2014-09-18 Lubrizol Advanced Materials, Inc. Itaconic acid polymers

Similar Documents

Publication Publication Date Title
Quidde et al. Detoxification of α-tomatine byBotrytis cinerea
US5906930A (en) Para-nitrobenzyl esterases with enhanced activity in aqueous and nonaqueous media
Aronsson et al. A simple method for isolating import‐competent Arabidopsis chloroplasts
Smith et al. The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii.
Kerr et al. Gene 6 exonuclease of bacteriophage T7: I. Purification and properties of the enzyme
Briganti et al. Purification, biochemical properties and substrate specificity of a catechol 1, 2-dioxygenase from a phenol degrading Acinetobacter radioresistens
Falkenstein et al. The 29 kb plasmid, common in strains of Erwinia amylovora, modulates development of fireblight symptoms
Miller et al. Stereoselective excision of thymine glycol from oxidatively damaged DNA
Garber et al. In vitro processing of B. mori transfer RNA precursor molecules
Akaboshi et al. Veal heart ribonuclease P has an essential RNA component
CA2264270A1 (en) Mass production of paclitaxel by changing the temperature of the medium during the plant cell culture
Bharati et al. Human mitochondrial uracil-DNA glycosylase preform (UNG1) is processed to two forms one of which is resistant to inhibition by AP sites
Knab et al. A Camptothecin-resistant DNA Topoisomerase I Mutant Exhibits Altered Sensitivities to Other DNA Topoisomerase Poisons (∗)
JPS6486879A (en) Dna fragment
Hsu et al. Effect of bacteriophage infection on the sulfur-labeling of sRNA.
LaMotta et al. Multiple forms of serum cholinesterase
Bacic et al. In vivo characterization of dimethylsulfoniopropionate lyase in the fungus Fusarium lateritium
Cloves et al. The mechanism of action of primary alkylsulphohydrolase and arylsulphohydrolase from a detergent-degrading micro-organism
WO1994000572A3 (en) Gene for ataxia-telangiectasia complementation group d (atdc)
Nabi et al. Bioluminescence assays with immobilised bacterial luciferase using flow injection analysis
Myers et al. Effects of the residue adjacent to the reactive serine on the substrate interactions of Drosophila esterase 6
Shiiba et al. Isolation of giant mitochondrial nucleoids from the yeast Saccharomyces cerevisiae
Ogawa et al. Group-I introns in the cytochrome c oxidase genes of Dictyostelium discoideum: two related ORFs in one loop of a group-I intron, a cox1/2 hybrid gene and an unusually large cox3 gene
ATE209683T1 (en) PLANT RESISTANT TO AT LEAST TWO VIRUSES AND ITS PREPARATION
Young et al. Changes in the levels of DNA-dependent RNA polymerases during the transition of the dimorphic fungus Mucor rouxii from yeast-like to mycelial growth