JPS55111786A - Immobilized enzyme composition of pyruvate oxidase - Google Patents

Immobilized enzyme composition of pyruvate oxidase

Info

Publication number
JPS55111786A
JPS55111786A JP1951979A JP1951979A JPS55111786A JP S55111786 A JPS55111786 A JP S55111786A JP 1951979 A JP1951979 A JP 1951979A JP 1951979 A JP1951979 A JP 1951979A JP S55111786 A JPS55111786 A JP S55111786A
Authority
JP
Japan
Prior art keywords
immobilized enzyme
pyruvate
pyruvate oxidase
enzyme composition
blended
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP1951979A
Other languages
Japanese (ja)
Inventor
Kunio Matsumoto
Hiroyuki Mizuguchi
Kozo Iida
Tsutomu Yamamoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kansai Paint Co Ltd
Toyo Jozo KK
Original Assignee
Kansai Paint Co Ltd
Toyo Jozo KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kansai Paint Co Ltd, Toyo Jozo KK filed Critical Kansai Paint Co Ltd
Priority to JP1951979A priority Critical patent/JPS55111786A/en
Publication of JPS55111786A publication Critical patent/JPS55111786A/en
Pending legal-status Critical Current

Links

Landscapes

  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

PURPOSE: To obtain enzyme compsn. useful as immobilized enzyme for analysis of a pyruvate-containing system or a pyruvate-free system, by a method wherein a thiol reagent is blended with immobilized enzyme of pyruvate oxidase included in phtot-setting resin.
CONSTITUTION: Pyruvate oxidase is uniformly mixed with nonionic prepolymer having at least two photo-polymerizable ethylenically unsaturated gp. per molecule in an aq. medium such as tris-hydrochloric acid buffer solution or phosphate buffer solution. The mixt. is irradiated with light of a wavelength of 250W600mμ for 10secW30min to obtain immobilized enzyme of pyruvate oxidaze included in photo- setting resin. This immobilized enzyme is blended with a thiol reagent such as mercaptoethanol, dithiothreitol or dithioerythritol as a stabilizer to obtain immobilized enzyme composition of pyruvate oxidase.
COPYRIGHT: (C)1980,JPO&Japio
JP1951979A 1979-02-20 1979-02-20 Immobilized enzyme composition of pyruvate oxidase Pending JPS55111786A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP1951979A JPS55111786A (en) 1979-02-20 1979-02-20 Immobilized enzyme composition of pyruvate oxidase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1951979A JPS55111786A (en) 1979-02-20 1979-02-20 Immobilized enzyme composition of pyruvate oxidase

Publications (1)

Publication Number Publication Date
JPS55111786A true JPS55111786A (en) 1980-08-28

Family

ID=12001587

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1951979A Pending JPS55111786A (en) 1979-02-20 1979-02-20 Immobilized enzyme composition of pyruvate oxidase

Country Status (1)

Country Link
JP (1) JPS55111786A (en)

Similar Documents

Publication Publication Date Title
Sinha Colorimetric assay of catalase
CA2184270A1 (en) Method for Suppressing Inhibition of Enzyme-Mediated Reactions by Ionic Detergents
AU4339196A (en) Use of exonuclease and/or glycosylase as supplements to anti-polymerase antibody to increase specificity in polymerase chain reaction
BR9603563A (en) Chemically modified thermostable enzyme process for its preparation process for the amplification of a nucleic acid reaction amplification mixture and kit to conduct a polymerase chain reaction
ES2045932T3 (en) PROCEDURE FOR THE DETECTION OF SPECIFIC NUCLEIC ACID SEQUENCES AND THEIR APPLICATIONS.
CA2210354A1 (en) Mutant luciferases
EP0756637B1 (en) Methods for calibrating chemical assays
BR9510408A (en) Improvement in or related to endometrial function
CA2133560A1 (en) Hair analysis method
CLARK et al. Conversion of the active‐site cysteine residue of papain into a dehydro‐serine, a serine and a glycine residue
Leutbecher et al. Glutamate mutase from Clostridium cochlearium: purification, cobamide content and stereospecific inhibitors
Schmeckpeper et al. Translation of immunoglobulin mRNAs in a wheat germ cell-free system
Ware et al. Physiological and chemical properties of a reductant-activated inorganic pyrophosphatase from Desulfovibrio desulfuricans
CA2199835A1 (en) Method for preparing nucleic acids for analysis and kits useful therefor
DE3470178D1 (en) Assay pretreatment method, compositon therefor and agglutination assay kit containing it
JPS55111786A (en) Immobilized enzyme composition of pyruvate oxidase
DK340882A (en) PROCEDURE FOR THE PREPARATION OF SUR PROTEASE AND MUTANTS OF THE GENE RHIZOPUS
Rhodes et al. Studies in helminth enzymology. III. Malic dehydrogenases of Ascaris suum
EP0751226A3 (en) Process for amplifying nucleic acid sequences
JPS578797A (en) Method and reagent for quantitative determination of free fatty acid
Makemson et al. Bovine serum albumin interacts with bacterial luciferase
DE3265522D1 (en) Method for preparing a chromogen reactive for determining the serum iron concentration and binding power, and the chromogen reactive obtained thereby
Gershbein et al. Total lactate dehydrogenase and its isoenzymes in serum in the presence of penicillamine and other sulfhydryl compounds.
JPS561896A (en) Determination of hydrogen peroxide and reagent for it
GB2043244A (en) A process for the determination of two or more components in a liquid sample and a reagent kit for carrying out such a photometric determination