JPS578797A - Method and reagent for quantitative determination of free fatty acid - Google Patents

Method and reagent for quantitative determination of free fatty acid

Info

Publication number
JPS578797A
JPS578797A JP4346381A JP4346381A JPS578797A JP S578797 A JPS578797 A JP S578797A JP 4346381 A JP4346381 A JP 4346381A JP 4346381 A JP4346381 A JP 4346381A JP S578797 A JPS578797 A JP S578797A
Authority
JP
Japan
Prior art keywords
reagent
free fatty
acyl
coenzyme
quantitative determination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP4346381A
Other languages
Japanese (ja)
Other versions
JPS5733955B2 (en
Inventor
Hiromichi Hirakawa
Heihachiro Kinoshita
Koji Mizuno
Mitsuyoshi Toyosato
Original Assignee
Nippon Shoji Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Shoji Kk filed Critical Nippon Shoji Kk
Priority to JP4346381A priority Critical patent/JPS5733955B2/ja
Publication of JPS578797A publication Critical patent/JPS578797A/en
Publication of JPS5733955B2 publication Critical patent/JPS5733955B2/ja
Expired legal-status Critical Current

Links

Abstract

PURPOSE: To carry out the quantitative determination of free fatty acids, by treating the free fatty acids with an acyl CoA synthetase in the presence of ATP and CoA, oxidizing the produced acyl CoA with an acyl CoA oxidase in the presence of an SH reagent, and determining the produced hydrogen peroxide.
CONSTITUTION: An aqueous solution or dispersion of an optionally diluted specimen is treated with an acyl coenzyme A synthetase, adenosine triphosphate, and coenzyme A at pH7W8 and 37°C for 5W60min. An acyl coenzyme A oxidase, an SH reagent such as a maleimide derivative, iodoacetamide, etc., peroxidase or catalase, and color-developing reagents such as 4-aminoantipyrin and phenol, etc. are added simultaneously or separately to the above treated product, and heated at 37°C for 5W60min. The density of the developed color is determined by the colorimetry using a blank test as a contrast.
COPYRIGHT: (C)1982,JPO&Japio
JP4346381A 1981-03-24 1981-03-24 Expired JPS5733955B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP4346381A JPS5733955B2 (en) 1981-03-24 1981-03-24

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4346381A JPS5733955B2 (en) 1981-03-24 1981-03-24

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP13701178A Division JPS5729998B2 (en) 1978-11-06 1978-11-06

Publications (2)

Publication Number Publication Date
JPS578797A true JPS578797A (en) 1982-01-18
JPS5733955B2 JPS5733955B2 (en) 1982-07-20

Family

ID=12664400

Family Applications (1)

Application Number Title Priority Date Filing Date
JP4346381A Expired JPS5733955B2 (en) 1981-03-24 1981-03-24

Country Status (1)

Country Link
JP (1) JPS5733955B2 (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0159870A2 (en) * 1984-04-13 1985-10-30 Kyowa Medex Co. Ltd. Method for the determination of mercapto compounds and reagent for use therein
JPH01249679A (en) * 1988-03-29 1989-10-04 Toyo Tanso Kk Graphite-silicon carbide composite body and production thereof
US5126246A (en) * 1988-04-15 1992-06-30 Toyo Jozo Company, Ltd. Reagent for analysis of triglycerides and analysis using the same
JP2002051769A (en) * 2000-08-11 2002-02-19 Azwell Inc Liquid reagent useful for measuring nonesterified fatty acid
US6686172B2 (en) 2000-06-30 2004-02-03 Azwell, Inc. Method of measuring total homocysteine
US10358257B2 (en) 2014-07-30 2019-07-23 Ball Corporation Vented container end closure
US10556718B2 (en) 2013-03-15 2020-02-11 Ball Corporation End closure with a ring pull actuated secondary vent

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0159870A2 (en) * 1984-04-13 1985-10-30 Kyowa Medex Co. Ltd. Method for the determination of mercapto compounds and reagent for use therein
US4978615A (en) * 1984-04-13 1990-12-18 Kyowa Medex Co., Ltd. Method for the determination of the compound having mercapto group
JPH01249679A (en) * 1988-03-29 1989-10-04 Toyo Tanso Kk Graphite-silicon carbide composite body and production thereof
JPH0583517B2 (en) * 1988-03-29 1993-11-26 Toyo Tanso Co
US5126246A (en) * 1988-04-15 1992-06-30 Toyo Jozo Company, Ltd. Reagent for analysis of triglycerides and analysis using the same
US6686172B2 (en) 2000-06-30 2004-02-03 Azwell, Inc. Method of measuring total homocysteine
JP2002051769A (en) * 2000-08-11 2002-02-19 Azwell Inc Liquid reagent useful for measuring nonesterified fatty acid
US10556718B2 (en) 2013-03-15 2020-02-11 Ball Corporation End closure with a ring pull actuated secondary vent
US10358257B2 (en) 2014-07-30 2019-07-23 Ball Corporation Vented container end closure
US10981694B2 (en) 2014-07-30 2021-04-20 Ball Corporation Vented container end closure

Also Published As

Publication number Publication date
JPS5733955B2 (en) 1982-07-20

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