DK1124944T3 - Mutant luciferase med foröget termostabilitet - Google Patents

Mutant luciferase med foröget termostabilitet

Info

Publication number
DK1124944T3
DK1124944T3 DK99950990T DK99950990T DK1124944T3 DK 1124944 T3 DK1124944 T3 DK 1124944T3 DK 99950990 T DK99950990 T DK 99950990T DK 99950990 T DK99950990 T DK 99950990T DK 1124944 T3 DK1124944 T3 DK 1124944T3
Authority
DK
Denmark
Prior art keywords
amino acid
acid residue
luciferase
photinus pyralis
residue corresponding
Prior art date
Application number
DK99950990T
Other languages
English (en)
Inventor
David James Squirrell
Melenie Jane Murphy
Rachel Louise Leslie
Christopher R Lowe
Peter John White
Laurence Carlo Tisi
James Augustus Henry Murray
Original Assignee
3M Innovative Properties Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 3M Innovative Properties Co filed Critical 3M Innovative Properties Co
Application granted granted Critical
Publication of DK1124944T3 publication Critical patent/DK1124944T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Luminescent Compositions (AREA)
  • Lubricants (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DK99950990T 1998-10-28 1999-10-26 Mutant luciferase med foröget termostabilitet DK1124944T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9823468.5A GB9823468D0 (en) 1998-10-28 1998-10-28 Novel enzyme
PCT/GB1999/003538 WO2000024878A2 (en) 1998-10-28 1999-10-26 Mutant luciferase having increased thermostability

Publications (1)

Publication Number Publication Date
DK1124944T3 true DK1124944T3 (da) 2009-04-14

Family

ID=10841350

Family Applications (2)

Application Number Title Priority Date Filing Date
DK99950990T DK1124944T3 (da) 1998-10-28 1999-10-26 Mutant luciferase med foröget termostabilitet
DK04000812T DK1479763T3 (da) 1998-10-28 1999-10-26 Mutant luciferase med foröget termistabilitet

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK04000812T DK1479763T3 (da) 1998-10-28 1999-10-26 Mutant luciferase med foröget termistabilitet

Country Status (11)

Country Link
US (4) US7906298B1 (da)
EP (9) EP2071023B1 (da)
JP (3) JP5185479B2 (da)
AT (2) ATE419335T1 (da)
AU (5) AU770722B2 (da)
CA (1) CA2342300C (da)
DE (2) DE69940208D1 (da)
DK (2) DK1124944T3 (da)
ES (2) ES2319941T3 (da)
GB (2) GB9823468D0 (da)
WO (1) WO2000024878A2 (da)

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WO2009142735A2 (en) 2008-05-19 2009-11-26 Promega Corporation LUCIFERASE BIOSENSORS FOR cAMP
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WO2011143339A1 (en) 2010-05-11 2011-11-17 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
JP5889910B2 (ja) 2010-11-02 2016-03-22 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 新規セレンテラジン基質及び使用法
PL2635583T3 (pl) 2010-11-02 2015-11-30 Promega Corp Pochodne koelenterazyny i metody ich wykorzystania
CN103582701B (zh) 2010-12-03 2018-08-14 基因流股份有限公司 改善的光-发射分子
JP5887071B2 (ja) * 2011-06-01 2016-03-16 出光興産株式会社 粒状物の貯蔵装置および水添石油樹脂ペレットの貯蔵装置
EP3467119B1 (en) * 2011-11-11 2022-02-23 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
AU2013204332B2 (en) 2012-04-16 2015-07-16 Commonwealth Scientific And Industrial Research Organisation Methods and systems for detecting an analyte or classifying a sample
US9487814B2 (en) 2013-02-22 2016-11-08 Promega Corporation Stabilized formulation for luminescent detection of luciferase and nucleoside phosphates
WO2015116806A1 (en) 2014-01-29 2015-08-06 Promega Corporation Pro-substrates for live cell applications
EP3099683B1 (en) 2014-01-29 2020-08-05 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
JP6811173B2 (ja) 2014-09-11 2021-01-13 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 増強された発光を産生するために赤外発光基質を活用するルシフェラーゼ配列
EP3204509B1 (en) 2014-10-08 2019-07-10 Promega Corporation Bioluminescent succinate detection assay
EP3056902B1 (en) 2015-02-16 2020-04-01 Universite De Bordeaux Novel voltage dependent ion channel fusions and method of use thereof
JP7175881B2 (ja) * 2016-10-13 2022-11-21 プロメガ コーポレイション ルシフェリン分解産物による阻害に対して改善された耐性を持つ熱安定性ルシフェラーゼ
US11952612B2 (en) 2016-11-14 2024-04-09 Commonwealth Scientific And Industrial Research Organisation Protease sensor molecules
JP6349003B1 (ja) 2017-03-17 2018-06-27 東洋ビーネット株式会社 耐熱性ルシフェラーゼ
US11662349B2 (en) 2017-08-08 2023-05-30 Commonwealth Scientific And Industrial Research Organisation Carbohydrate sensors
AU2018353813A1 (en) 2017-10-18 2020-04-09 Domain Therapeutics Systems and methods for the assessment of G-protein activation
CN115087743A (zh) 2020-02-14 2022-09-20 龟甲万株式会社 用于测定样本中的atp、以及amp和/或adp的液体组合物
EP3882632A1 (en) 2020-03-18 2021-09-22 Universite De Bordeaux Novel ion conducting channel fusion subunits and methods of use thereof
WO2024158050A1 (ja) * 2023-01-27 2024-08-02 キッコーマン株式会社 ルシフェラーゼ変異体

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Also Published As

Publication number Publication date
EP2366778A1 (en) 2011-09-21
EP2368976A1 (en) 2011-09-28
JP5185479B2 (ja) 2013-04-17
ATE419335T1 (de) 2009-01-15
AU2004202277B2 (en) 2008-05-01
US20140170687A1 (en) 2014-06-19
JP2012161325A (ja) 2012-08-30
US20140170686A1 (en) 2014-06-19
AU6357399A (en) 2000-05-15
AU2004200277B2 (en) 2005-11-24
AU2004202277A1 (en) 2004-06-17
JP5951325B2 (ja) 2016-07-13
EP2071023A8 (en) 2010-06-02
DE69940208D1 (de) 2009-02-12
AU770722B2 (en) 2004-02-26
AU2004202276B2 (en) 2008-05-01
GB9823468D0 (en) 1998-12-23
US20110177540A1 (en) 2011-07-21
EP2366778B1 (en) 2012-08-22
GB2345913A (en) 2000-07-26
ATE418602T1 (de) 2009-01-15
EP2071023A2 (en) 2009-06-17
WO2000024878A2 (en) 2000-05-04
DK1479763T3 (da) 2009-04-06
EP1124944A2 (en) 2001-08-22
AU2008203449A1 (en) 2008-08-28
EP2366779A1 (en) 2011-09-21
AU2004202276A1 (en) 2004-06-17
GB9925162D0 (en) 1999-12-22
US7906298B1 (en) 2011-03-15
AU2004200277A1 (en) 2004-02-19
EP2366780B1 (en) 2018-06-13
EP2366779B1 (en) 2018-07-11
EP2366780A1 (en) 2011-09-21
EP1479763B1 (en) 2008-12-24
EP1479763A3 (en) 2005-05-25
CA2342300A1 (en) 2000-05-04
EP2071023B1 (en) 2016-04-27
EP2366777A1 (en) 2011-09-21
EP2368977A1 (en) 2011-09-28
ES2319941T3 (es) 2009-05-14
JP2011120605A (ja) 2011-06-23
DE69940172D1 (de) 2009-02-05
CA2342300C (en) 2009-08-11
EP1479763A2 (en) 2004-11-24
WO2000024878A3 (en) 2000-08-03
JP2003518912A (ja) 2003-06-17
US8652794B2 (en) 2014-02-18
EP1124944B1 (en) 2008-12-31
EP2368977B1 (en) 2018-06-27
GB2345913B (en) 2004-03-17
EP2366777B1 (en) 2018-07-25
ES2319520T3 (es) 2009-05-08
EP2071023A3 (en) 2009-10-14

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