PL3498709T3 - Lucyferazy otrzymane z krewetki oplophorus, nowe substraty koelenterazyny oraz sposoby stosowania - Google Patents

Lucyferazy otrzymane z krewetki oplophorus, nowe substraty koelenterazyny oraz sposoby stosowania

Info

Publication number
PL3498709T3
PL3498709T3 PL19150036.2T PL19150036T PL3498709T3 PL 3498709 T3 PL3498709 T3 PL 3498709T3 PL 19150036 T PL19150036 T PL 19150036T PL 3498709 T3 PL3498709 T3 PL 3498709T3
Authority
PL
Poland
Prior art keywords
oplophorus
luciferases
shrimp
methods
new coelenterazine
Prior art date
Application number
PL19150036.2T
Other languages
English (en)
Inventor
Brock Binkowski
Lance P. Encell
Mary P. Hall
Matthew B. Robers
Michael R. Slater
Keith V. Wood
Monika G. Wood
Dieter Klaubert
Poncho Meisenheimer
James Unch
Michael P. Valley
Original Assignee
Promega Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44983728&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PL3498709(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Promega Corporation filed Critical Promega Corporation
Publication of PL3498709T3 publication Critical patent/PL3498709T3/pl

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D487/00Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04Ortho-condensed systems
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K19/00Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1086Preparation or screening of expression libraries, e.g. reporter assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0069Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y113/00Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12005Renilla-luciferin 2-monooxygenase (1.13.12.5), i.e. renilla-luciferase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y113/00Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y113/00Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12013Oplophorus-luciferin 2-monooxygenase (1.13.12.13)
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/07Animals genetically altered by homologous recombination
    • A01K2217/072Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
PL19150036.2T 2010-11-02 2011-11-02 Lucyferazy otrzymane z krewetki oplophorus, nowe substraty koelenterazyny oraz sposoby stosowania PL3498709T3 (pl)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US40942210P 2010-11-02 2010-11-02

Publications (1)

Publication Number Publication Date
PL3498709T3 true PL3498709T3 (pl) 2026-03-23

Family

ID=44983728

Family Applications (3)

Application Number Title Priority Date Filing Date
PL19150036.2T PL3498709T3 (pl) 2010-11-02 2011-11-02 Lucyferazy otrzymane z krewetki oplophorus, nowe substraty koelenterazyny oraz sposoby stosowania
PL11785534T PL2635595T3 (pl) 2010-11-02 2011-11-02 Lucyferazy otrzymane z krewetki oplophorus, nowe substraty koelenterazyny oraz sposoby stosowania
PL11782732T PL2635582T3 (pl) 2010-11-02 2011-11-02 Nowe substraty będące koelenterazynami i sposoby zastosowania

Family Applications After (2)

Application Number Title Priority Date Filing Date
PL11785534T PL2635595T3 (pl) 2010-11-02 2011-11-02 Lucyferazy otrzymane z krewetki oplophorus, nowe substraty koelenterazyny oraz sposoby stosowania
PL11782732T PL2635582T3 (pl) 2010-11-02 2011-11-02 Nowe substraty będące koelenterazynami i sposoby zastosowania

Country Status (15)

Country Link
US (11) US8669103B2 (pl)
EP (4) EP2635582B1 (pl)
JP (6) JP5889910B2 (pl)
KR (2) KR101939553B1 (pl)
CN (3) CN103443121B (pl)
AU (2) AU2011323418B2 (pl)
BR (2) BR112013010855A2 (pl)
CA (3) CA2815227C (pl)
DK (2) DK2635582T3 (pl)
ES (2) ES2567410T3 (pl)
IL (5) IL225867B (pl)
PL (3) PL3498709T3 (pl)
PT (1) PT2635595T (pl)
SG (5) SG10201607774RA (pl)
WO (2) WO2012061530A2 (pl)

Families Citing this family (88)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007508014A (ja) 2003-10-10 2007-04-05 プロメガ コーポレイション ルシフェラーゼバイオセンサー
EP2004813B1 (en) 2006-04-03 2015-06-03 Promega Corporation Permuted and nonpermuted luciferase biosensors binding cyclic nucleotides
ATE542903T1 (de) 2008-05-19 2012-02-15 Promega Corp Luciferase-biosensoren für camp
SG10201901453PA (en) * 2009-05-01 2019-03-28 Promega Corp Synthetic oplophorus luciferases with enhanced light output
US9290794B2 (en) * 2010-05-11 2016-03-22 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
WO2011143339A1 (en) 2010-05-11 2011-11-17 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
CN103443121B (zh) * 2010-11-02 2016-04-20 普罗梅加公司 新型腔肠素底物及其使用方法
WO2012061477A1 (en) 2010-11-02 2012-05-10 Promega Corporation Coelenterazine derivatives and methods of using same
WO2013078244A1 (en) 2011-11-21 2013-05-30 Promega Corporation Carboxy x rhodamine analogs
US10067149B2 (en) 2012-12-12 2018-09-04 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
KR102227321B1 (ko) 2012-12-12 2021-03-12 프로메가 코포레이션 생체활성 제제의 세포 표적 포획을 위한 조성물 및 방법
KR102150479B1 (ko) * 2012-12-12 2020-09-01 프로메가 코포레이션 세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식
JP6511720B2 (ja) * 2013-02-28 2019-05-15 Jnc株式会社 コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法
US11072811B2 (en) 2013-03-15 2021-07-27 Promega Corporation Substrates for covalent tethering of proteins to functional groups or solid surfaces
US10168323B2 (en) 2013-03-15 2019-01-01 Promega Corporation Compositions and methods for capture of cellular targets of bioactive agents
US9797890B2 (en) 2013-03-15 2017-10-24 Promega Corporation Activation of bioluminescence by structural complementation
US9340817B2 (en) 2013-03-27 2016-05-17 Sample6 Technologies, Inc. Methods of making recombinant phage, compositions and articles of manufacture of same for bacterial detection
JP6040846B2 (ja) * 2013-04-08 2016-12-07 Jnc株式会社 セレンテラジン類縁体
US9828625B2 (en) 2013-06-19 2017-11-28 Phage Diagnostics, Inc. Phage-based bacterial detection assay
KR102445330B1 (ko) 2013-07-12 2022-09-19 더 칠드런스 호스피탈 오브 필라델피아 Aav 벡터 및 항―aav (아데노-관련 바이러스) 중화 항체에 대한 검정
CN105555964B (zh) 2013-07-18 2020-04-17 洛桑联邦理工学院 生物样品中进行生物发光共振能量转移(bret)分析的方式和方法
JP6484987B2 (ja) * 2013-10-21 2019-03-20 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
WO2015067302A1 (en) 2013-11-05 2015-05-14 Ecole Polytechnique Federale De Lausanne (Epfl) Sensor molecules and uses thereof
JP6442825B2 (ja) 2013-12-26 2018-12-26 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
WO2015116867A1 (en) 2014-01-29 2015-08-06 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
US9927430B2 (en) 2014-01-29 2018-03-27 Promega Corporation Pro-substrates for live cell applications
JP6256118B2 (ja) * 2014-03-11 2018-01-10 Jnc株式会社 エビルシフェラーゼの触媒蛋白質のキメラ遺伝子及びその使用法
JP6265020B2 (ja) 2014-04-16 2018-01-24 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
BR112016027504B1 (pt) 2014-05-23 2023-04-18 Promega Corporation Composição e método para estabilizar um substrato luminogênico e kit que compreende a referida composição
WO2016040788A1 (en) 2014-09-11 2016-03-17 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
US11365402B2 (en) * 2014-09-12 2022-06-21 Promega Corporation Internal protein tags
JP6814132B2 (ja) * 2014-09-12 2021-01-13 プロメガ コーポレイションPromega Corporation 内部タンパク質タグ
US20190054117A1 (en) * 2014-12-19 2019-02-21 Novartis Ag Dimerization switches and uses thereof
EP3254113B1 (en) 2015-02-05 2024-10-09 Promega Corporation Luciferase-based thermal shift assays
EP3304078B1 (en) 2015-06-05 2022-08-31 Promega Corporation Cell-permeable, cell-compatible, and cleavable linkers for covalent tethering of functional elements
WO2016210294A1 (en) 2015-06-25 2016-12-29 Promega Corporation Thienopyrrole compounds and uses thereof as inhibitors of oplophorus-derived luciferases
US20170029798A1 (en) * 2015-07-27 2017-02-02 Cellivery Therapeutics, Inc. Development of Improved Cell-Permeable (iCP) Parkin Recombinant Protein as a Protein-Based Anti-Neurodegenerative Agent for the Treatment of Parkinson's Disease-Associated Phenotypes by Utilizing BBB-Penetrating Protein Delivery System MITT, Enabled by Advanced Macromolecule Transduction Domain (aMTD)
WO2017142852A1 (en) 2016-02-15 2017-08-24 Promega Corporation Coelenterazine analogues
US11015176B2 (en) 2016-03-11 2021-05-25 National Institute Of Advanced Industrial Science And Technology Nucleic acid encoding a luminescent enzyme protein
WO2017189751A1 (en) 2016-04-26 2017-11-02 The University Of Utah Research Foundation Target-binding activated split reporter systems for analyte detection and related components and methods
CN105968114B (zh) * 2016-05-27 2018-11-09 山东大学 一种腔肠素类似物及其制备方法与应用
JP7212612B2 (ja) * 2016-07-28 2023-01-25 プロメガ コーポレイション セレンテラジン類縁体
US10202584B2 (en) * 2016-09-01 2019-02-12 The Regents Of The University Of California Red-shifted luciferase-luciferin pairs for enhanced bioluminescence
EP3510035B1 (en) 2016-09-09 2021-04-07 Promega Corporation Dual protected pro-coelenterazine substrates
US10280447B2 (en) 2016-11-01 2019-05-07 Promega Corporation Substituted imidazo[1,2-A]pyrazines tethered to energy acceptors
EP3548470B1 (en) * 2016-12-01 2023-11-01 Promega Corporation 5,5-disubstituted luciferins and their use in luciferase-based assays
DK3548492T5 (en) * 2016-12-01 2024-08-05 Promega Corp Cell impermeable coelenterazine analogues
JP7161475B2 (ja) 2016-12-28 2022-10-26 プロメガ コーポレイション 官能化nanoluc阻害剤
EP3395803A1 (en) 2017-04-28 2018-10-31 Institut Pasteur Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins
US11959121B2 (en) 2017-05-30 2024-04-16 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Sensors, methods and kits for detecting NADPH based on resonance energy transfer
WO2019010094A1 (en) 2017-07-06 2019-01-10 Promega Corporation STERILE SERIES PRO-COELENTERAZINE ANALOGS
CN108107209B (zh) * 2017-09-19 2020-07-24 中国中医科学院医学实验中心 一种检测分泌型报告蛋白含量的优化方法及其所用生物材料
WO2019213119A1 (en) * 2018-05-01 2019-11-07 Promega Corporation Coelenterazine compounds as nanoluc suicide substrates
WO2019232225A1 (en) 2018-05-30 2019-12-05 Promega Corporation Broad-spectrum kinase binding agents
EP3802514B1 (en) 2018-06-01 2022-08-03 Promega Corporation Inhibitors of oplophorus luciferase-derived bioluminescent complexes
WO2019241438A2 (en) * 2018-06-12 2019-12-19 Promega Corporation Multipartite luciferase
US10962538B2 (en) 2018-06-14 2021-03-30 The Trustees Of Columbia University In The City Of New York Assays using arrestin recruitment and unmodified receptors
EP3829648A1 (en) 2018-07-27 2021-06-09 Promega Corporation Quinone-containing conjugates
ES3052851T3 (en) * 2018-08-23 2026-01-15 Promega Corp Coelenterazine analogs for use in luciferase-based assays
CN119955899A (zh) 2018-10-03 2025-05-09 普洛麦格公司 用于稳定腔肠素及其类似物和衍生物的组合物、方法和试剂盒
US11175228B2 (en) 2018-11-28 2021-11-16 Promega Corporation Reactive peptide labeling
EP3891503A2 (en) 2018-12-04 2021-10-13 Promega Corporation Broad spectrum gpcr binding agents
US20220259574A1 (en) * 2019-02-27 2022-08-18 University Of Virginia Patent Foundation Atp-independent bioluminescent reporter variants to improve in vivo imaging
US11913944B2 (en) 2019-03-20 2024-02-27 Promega Corporation Photoaffinity probes
US11360096B2 (en) 2019-05-16 2022-06-14 Duke University Complex BRET technique for measuring biological interactions
EP3986956A1 (en) 2019-06-24 2022-04-27 Promega Corporation Modified polyamine polymers for delivery of biomolecules into cells
CN115427556A (zh) * 2019-11-27 2022-12-02 普洛麦格公司 多部分萤光素酶肽和多肽
EP4073512B1 (en) 2019-12-10 2025-11-12 Promega Corporation Compositions and methods for bioluminescent detection using multifunctional probes
JP7790724B2 (ja) * 2020-03-17 2025-12-23 国立研究開発法人産業技術総合研究所 発光基質化合物
EP3882632A1 (en) 2020-03-18 2021-09-22 Universite De Bordeaux Novel ion conducting channel fusion subunits and methods of use thereof
CN113527231A (zh) * 2020-04-15 2021-10-22 深圳华大生命科学研究院 杂环取代腔肠素类化合物的合成方法
EP3904508A1 (en) 2020-04-27 2021-11-03 Institut Pasteur Luciferase linked immunosorbent assay
JP7677997B2 (ja) 2020-05-22 2025-05-15 プロメガ コーポレイション キナーゼターゲットエンゲージメントの増強
US20220064696A1 (en) 2020-08-28 2022-03-03 Promega Corporation Target engagement assay for ras proteins
JPWO2022138892A1 (pl) 2020-12-25 2022-06-30
CN118703457A (zh) * 2021-04-01 2024-09-27 深圳华大生命科学研究院 一种刺虾荧光素酶Nluc的突变体及其应用
AU2022333242A1 (en) * 2021-08-24 2024-03-07 Centre National De La Recherche Scientifique Luminescent based antigen assay
EP4519426A2 (en) 2022-05-04 2025-03-12 Promega Corporation Split modified dehalogenase variants
JP2025515176A (ja) 2022-05-04 2025-05-13 プロメガ コーポレイション 光活性化可能な化合物及びその使用
EP4519425A1 (en) 2022-05-04 2025-03-12 Promega Corporation Modified dehalogenase with extended surface loop regions
EP4519290A1 (en) 2022-05-04 2025-03-12 Promega Corporation Circularly permuted dehalogenase variants
EP4549570A1 (en) * 2022-06-30 2025-05-07 DGI Tech (Qing Dao) Co., Limited Luciferase mutant and use thereof
CN115349611B (zh) * 2022-08-05 2023-10-24 江南大学 一种改善南美白对虾虾糜凝胶品质的方法
CN116068198B (zh) * 2022-11-30 2024-01-09 深圳湾实验室 Ppi原位检测方法及其载体、诊断试剂、试剂盒和应用
WO2024206099A2 (en) * 2023-03-24 2024-10-03 Promega Corporation Bioluminescence-triggered photocatalytic activation
JP7482563B1 (ja) 2023-06-21 2024-05-14 国立大学法人大阪大学 シイノトモシビタケ由来の発光関連遺伝子
DE102023120682B3 (de) 2023-08-03 2024-10-24 Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut Zytotoxizitätstest zum Nachweis von Zellschäden
GB202404021D0 (en) 2024-03-20 2024-05-01 Ipsen Biopharm Ltd Cell-based neurotoxin assay

Family Cites Families (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0134108B1 (en) 1983-08-17 1989-04-05 AlliedSignal Inc. Gas generator and method of generating gas
JPH0515398A (ja) 1991-07-15 1993-01-26 Nippon Oil & Fats Co Ltd オキシダーゼ酵素の定量方法
US5368484A (en) 1992-05-22 1994-11-29 Atari Games Corp. Vehicle simulator with realistic operating feedback
US6387675B1 (en) 1994-01-03 2002-05-14 Promega Corporation Mutant luciferases
AU698424C (en) 1994-01-03 2002-10-10 Promega Corporation Mutant luciferases
GB9501170D0 (en) 1994-03-23 1995-03-08 Secr Defence Luciferases
JP3648763B2 (ja) 1994-08-23 2005-05-18 日本油脂株式会社 ウミホタルルシフェリン誘導体および糖加水分解酵素の定量方法
GB9417593D0 (en) 1994-09-01 1994-10-19 Secr Defence Luciferase labelling method
US5670356A (en) 1994-12-12 1997-09-23 Promega Corporation Modified luciferase
IN186115B (pl) 1995-01-20 2001-06-23 Secr Defence Brit
JPH08294397A (ja) 1995-04-27 1996-11-12 Nippon Oil & Fats Co Ltd 免疫学的活性物質の測定方法
US5998204A (en) 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
GB9707486D0 (en) 1997-04-11 1997-05-28 Secr Defence Enzyme assays
CA2285571C (en) 1997-04-16 2008-12-02 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Immunomodulatory molecules and process for producing the same
CA2301828A1 (en) * 1997-09-19 1999-03-25 Promega Corporation Thermostable luciferases and methods of production
US6602677B1 (en) 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
GB9823468D0 (en) 1998-10-28 1998-12-23 Secr Defence Novel enzyme
JP3694181B2 (ja) 1999-01-05 2005-09-14 キッコーマン株式会社 変異型ルシフェラーゼ、変異型ルシフェラーゼ遺伝子、新規な組み換え体dna及び変異型ルシフェラーゼの製造法
GB9925161D0 (en) 1999-10-26 1999-12-22 Secr Defence Novel enzyme
NZ518413A (en) 1999-10-26 2004-11-26 Secr Defence Novel enzyme
US20010046687A1 (en) 2000-03-31 2001-11-29 Dicesare Joseph L. Method for the enhancement of luminescence intensity from a reaction between adenosine triphosphate (ATP) and a luciferase/luciferin reactant system
EP1156103B1 (en) 2000-04-26 2010-08-11 Chisso Corporation Oplophorus luciferase
JP4613441B2 (ja) * 2000-04-26 2011-01-19 チッソ株式会社 新規ルシフェラーゼおよび発光蛋白質
US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
US7268229B2 (en) 2001-11-02 2007-09-11 Promega Corporation Compounds to co-localize luminophores with luminescent proteins
AU2003216139B2 (en) 2002-02-01 2008-08-14 Promega Corporation Bioluminescent protease assay
EP1546162B1 (en) 2002-09-20 2011-06-22 Promega Corporation Luminescence-based methods and probes for measuring cytochrome p450 activity
ES2237643T3 (es) * 2002-10-21 2005-08-01 Axxam S.R.L. Fotoproteina con bioluminiscencia mejorada.
JP4485470B2 (ja) 2002-12-23 2010-06-23 プロメガ コーポレイション ルシフェラーゼ酵素活性を保護するための方法及びキット
US7238842B2 (en) 2003-01-31 2007-07-03 Promega Corporation Covalent tethering of functional groups to proteins
US7429472B2 (en) 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
CA2515217A1 (en) 2003-02-12 2004-08-26 Promega Corporation Methods and kits for dual enzymatic assays whereby light is quenched from luminescent reactions
US20040178545A1 (en) 2003-03-14 2004-09-16 Cates Larry E. System for optically analyzing a molten metal bath
JP2007508014A (ja) 2003-10-10 2007-04-05 プロメガ コーポレイション ルシフェラーゼバイオセンサー
WO2005068483A1 (en) 2004-01-08 2005-07-28 Universität Zürich Metal complexes having vitamin b12 as a ligand
US7553632B2 (en) 2004-01-22 2009-06-30 Promega Corporation Luminogenic and nonluminogenic multiplex assay
US7416854B2 (en) 2004-01-22 2008-08-26 Promega Corporation Luminogenic and nonluminogenic multiplex assay
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
EP3179252B1 (en) 2004-07-30 2018-11-14 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US20070087400A1 (en) 2004-07-30 2007-04-19 Aldis Darzins Covalent tethering of functional groups to proteins and substrates therefor
US7728118B2 (en) 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
JP4639904B2 (ja) 2005-03-30 2011-02-23 チッソ株式会社 蛍光活性を有するルシフェラーゼの活性増強方法
JP3844082B2 (ja) * 2005-04-08 2006-11-08 キッコーマン株式会社 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体dna及び耐熱性ホタルルシフェラーゼの製造法
EP2272972A1 (en) 2005-05-31 2011-01-12 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
EP1935986B1 (en) 2005-05-31 2014-04-16 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
US7495579B2 (en) 2005-06-13 2009-02-24 Sirota J Marcos Traffic light status remote sensor system
AU2006281012A1 (en) 2005-08-17 2007-02-22 Yeda Research And Development Co. Ltd. Methods and compositions for determining a level of biologically active serum paraoxonase
JP4999341B2 (ja) * 2005-09-06 2012-08-15 株式会社バイオエネックス 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法
EP2004813B1 (en) 2006-04-03 2015-06-03 Promega Corporation Permuted and nonpermuted luciferase biosensors binding cyclic nucleotides
JP2007306871A (ja) 2006-05-19 2007-11-29 Chisso Corp 封入体可溶化方法及び精製方法
EP2374875B1 (en) 2006-10-30 2015-09-02 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and funcitional expression
WO2008086035A2 (en) 2007-01-10 2008-07-17 Promega Corporation Split mutant hydrolase fusion reporter and uses thereof
WO2008118445A1 (en) 2007-03-26 2008-10-02 Promega Corporation Methods to quench light from optical reactions
JP2011502509A (ja) 2007-11-05 2011-01-27 プロメガ コーポレイション ハイブリッド融合レポーター及びその使用
ATE542903T1 (de) 2008-05-19 2012-02-15 Promega Corp Luciferase-biosensoren für camp
EP2326953B1 (en) 2008-07-22 2018-03-21 Promega Corporation Adp detection based luminescent phosphotransferase or atp hydrolase assay
TWI375901B (en) 2008-08-05 2012-11-01 Elan Microelectronics Corp Touch screen and method for positioning coordinate
JP5582505B2 (ja) * 2009-02-09 2014-09-03 Jnc株式会社 セレンテラジン類縁体及びその製造方法
WO2010119721A1 (ja) * 2009-04-17 2010-10-21 独立行政法人産業技術総合研究所 超高輝度で安定な人工生物発光酵素
SG10201901453PA (en) * 2009-05-01 2019-03-28 Promega Corp Synthetic oplophorus luciferases with enhanced light output
WO2011038219A1 (en) 2009-09-24 2011-03-31 Promega Corporation Proteases having improved stability, solubility and activity
JP5605727B2 (ja) * 2010-04-06 2014-10-15 Jnc株式会社 セレンテラジン類縁体及びセレンテラミド類縁体
WO2011143339A1 (en) 2010-05-11 2011-11-17 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
JP6041442B2 (ja) 2010-09-01 2016-12-07 プロメガ コーポレイションPromega Corporation 酸化型グルタチオンアッセイ
US9284188B2 (en) 2010-09-03 2016-03-15 The Regents Of The University Of California Cytosolic delivery of materials with endosome-disrupting colloids
CN103443121B (zh) 2010-11-02 2016-04-20 普罗梅加公司 新型腔肠素底物及其使用方法
WO2012061477A1 (en) * 2010-11-02 2012-05-10 Promega Corporation Coelenterazine derivatives and methods of using same
US9273343B2 (en) 2011-09-02 2016-03-01 Promega Corporation Compounds and methods for assaying redox state of metabolically active cells and methods for measuring NAD(P)/NAD(P)H

Also Published As

Publication number Publication date
SG190104A1 (en) 2013-06-28
KR20130099997A (ko) 2013-09-06
KR20130084312A (ko) 2013-07-24
CA2817102C (en) 2020-07-28
US9951373B2 (en) 2018-04-24
AU2011323418B2 (en) 2017-07-27
AU2016213854B2 (en) 2018-01-04
DK2635582T3 (da) 2016-03-14
JP6155424B2 (ja) 2017-07-05
US9938564B2 (en) 2018-04-10
IL288070A (en) 2022-01-01
EP3498710A1 (en) 2019-06-19
JP2014500011A (ja) 2014-01-09
US8809529B2 (en) 2014-08-19
US20120174242A1 (en) 2012-07-05
WO2012061529A1 (en) 2012-05-10
DK2635595T3 (en) 2019-03-04
SG10201607774RA (en) 2016-11-29
US20260028662A1 (en) 2026-01-29
IL258790A (en) 2018-06-28
SG10202103336SA (en) 2021-04-29
IL226114A0 (en) 2013-06-27
KR101939553B1 (ko) 2019-01-17
CA2815227A1 (en) 2012-05-10
JP2016145219A (ja) 2016-08-12
JP6539689B2 (ja) 2019-07-03
IL288070B2 (en) 2023-02-01
CN103443121B (zh) 2016-04-20
PL2635595T4 (pl) 2019-04-30
US10774364B2 (en) 2020-09-15
ES2723773T3 (es) 2019-09-02
CN106148362A (zh) 2016-11-23
CN106148362B (zh) 2021-04-30
CN103370319A (zh) 2013-10-23
JP2016144454A (ja) 2016-08-12
JP2014503485A (ja) 2014-02-13
WO2012061530A3 (en) 2012-09-27
US20150064731A1 (en) 2015-03-05
EP2635595B1 (en) 2019-01-09
AU2016213854A1 (en) 2016-09-01
JP5889910B2 (ja) 2016-03-22
JP6374420B2 (ja) 2018-08-15
SG189496A1 (en) 2013-05-31
WO2012061530A8 (en) 2013-06-06
BR112013010855A2 (pt) 2017-06-27
US20140223590A1 (en) 2014-08-07
IL225867A0 (en) 2013-07-31
IL225867B (en) 2018-07-31
EP3498709C0 (en) 2025-12-31
US20220348983A1 (en) 2022-11-03
US20120117667A1 (en) 2012-05-10
EP2635595A2 (en) 2013-09-11
IL288070B (en) 2022-10-01
ES2567410T3 (es) 2016-04-22
US11661623B2 (en) 2023-05-30
IL258790B (en) 2020-05-31
CA2817102A1 (en) 2012-05-10
IL274316B (en) 2021-12-01
JP2018127459A (ja) 2018-08-16
EP2635582A1 (en) 2013-09-11
PL2635595T3 (pl) 2019-04-30
IL226114B (en) 2018-04-30
CA3081115A1 (en) 2012-05-10
AU2011323419A1 (en) 2013-05-30
JP6752839B2 (ja) 2020-09-09
EP3498709A1 (en) 2019-06-19
US20180327810A1 (en) 2018-11-15
JP6574721B2 (ja) 2019-09-11
EP3498709B1 (en) 2025-12-31
US9139836B2 (en) 2015-09-22
WO2012061530A2 (en) 2012-05-10
AU2011323418A1 (en) 2013-05-30
US9840730B2 (en) 2017-12-12
US20240158831A1 (en) 2024-05-16
PL2635582T3 (pl) 2016-08-31
SG10201608550SA (en) 2016-12-29
BR112013010487A2 (pt) 2016-11-22
PT2635595T (pt) 2019-01-29
IL274316A (en) 2020-06-30
CN103370319B (zh) 2016-08-24
CN103443121A (zh) 2013-12-11
US12435354B2 (en) 2025-10-07
US20140227759A1 (en) 2014-08-14
CA2815227C (en) 2019-03-26
US20160002703A1 (en) 2016-01-07
US8669103B2 (en) 2014-03-11
US20200399676A1 (en) 2020-12-24
US12215375B2 (en) 2025-02-04
KR101538624B1 (ko) 2015-07-22
JP2017176182A (ja) 2017-10-05
EP2635582B1 (en) 2016-01-27
BR112013010487B1 (pt) 2021-02-02

Similar Documents

Publication Publication Date Title
PL3498709T3 (pl) Lucyferazy otrzymane z krewetki oplophorus, nowe substraty koelenterazyny oraz sposoby stosowania
HUS1900007I1 (hu) Kiméra, antigénreceptor-módosított T-sejt alkalmazása rák kezelésére
IL225869A (en) Colentrazine derivatives and methods for their use
IL223295A (en) Lung cancer biomarkers and uses thereof
HUE056636T2 (hu) Cisz-1,1,1,4,4,4-hexafluor-2-butén azeotróp-jellegû készítményei
ME03766B (me) Određeni amino-pirimidini, njihove kompozicije, i metode njihove upotrebe
PL2552959T3 (pl) Przeciwciała przeciwko MUC16 i metody stosowania
LT2755473T (lt) Paraziticidinės kompozicijos, apimančios izoksazolino veiklųjį agentą, būdai ir jų panaudojimai
FI20105572A0 (fi) Muokattu beeta-laktamaasi ja siihen liittyvät menetelmät ja käytöt
AP2011005671A0 (en) Herbicide-resistant AHAS-mutants and methods of use.
PL3296319T3 (pl) Sposoby leczenia nowotworu jelita grubego
ME02952B (me) Wnt antagonisti i metode tretiranja
PL2430452T3 (pl) Diagnoza i leczenie raka w oparciu o genotyp odc1
ZA201308268B (en) Herbicidal composition comprising pinoxaden and fluroxypyrester, and methods of use thereof
EP2691396A4 (en) ENOPEPTINES, USES THEREOF AND SYNTHESIS METHOD THEREFOR
BR112012019385A2 (pt) luminária com porta giratória e removível.
BR112013015319A2 (pt) revestimento de autodeposição de aspecto brilhante aperfeiçoado, e métodos de aplicar o mesmo
WO2013177255A3 (en) Chimeric luciferases
FR2938406B3 (fr) Bracelet avec fermoir magnetique
IT1400480B1 (it) Pomata per bruciature e procedimento per il suo ottenimento.
GB201007197D0 (en) Methods and kits for determining the toxicity of an agent
ITRM20110584A1 (it) Metodi e composizioni.
EP2668944A4 (en) AROMATASE ACTIVATOR
UA52379U (ru) Применение ЛИПИНА в качестве средства фригопротекторного действия
UA52378U (ru) Применение ЛИПОФЛАВОНА в качестве средства фригопротекторного действия