JP2020508661A - 核酸ベースのデータ保存 - Google Patents
核酸ベースのデータ保存 Download PDFInfo
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- JP2020508661A JP2020508661A JP2019545334A JP2019545334A JP2020508661A JP 2020508661 A JP2020508661 A JP 2020508661A JP 2019545334 A JP2019545334 A JP 2019545334A JP 2019545334 A JP2019545334 A JP 2019545334A JP 2020508661 A JP2020508661 A JP 2020508661A
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- oligonucleotides
- bioencryption
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- nucleic acid
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Classifications
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06F—ELECTRIC DIGITAL DATA PROCESSING
- G06F21/00—Security arrangements for protecting computers, components thereof, programs or data against unauthorised activity
- G06F21/60—Protecting data
- G06F21/62—Protecting access to data via a platform, e.g. using keys or access control rules
- G06F21/6218—Protecting access to data via a platform, e.g. using keys or access control rules to a system of files or objects, e.g. local or distributed file system or database
-
- G—PHYSICS
- G11—INFORMATION STORAGE
- G11C—STATIC STORES
- G11C11/00—Digital stores characterised by the use of particular electric or magnetic storage elements; Storage elements therefor
- G11C11/54—Digital stores characterised by the use of particular electric or magnetic storage elements; Storage elements therefor using elements simulating biological cells, e.g. neuron
-
- G—PHYSICS
- G11—INFORMATION STORAGE
- G11C—STATIC STORES
- G11C13/00—Digital stores characterised by the use of storage elements not covered by groups G11C11/00, G11C23/00, or G11C25/00
- G11C13/0002—Digital stores characterised by the use of storage elements not covered by groups G11C11/00, G11C23/00, or G11C25/00 using resistive RAM [RRAM] elements
- G11C13/0009—RRAM elements whose operation depends upon chemical change
- G11C13/0014—RRAM elements whose operation depends upon chemical change comprising cells based on organic memory material
- G11C13/0019—RRAM elements whose operation depends upon chemical change comprising cells based on organic memory material comprising bio-molecules
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
- G16B50/30—Data warehousing; Computing architectures
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
- G16B50/40—Encryption of genetic data
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04L—TRANSMISSION OF DIGITAL INFORMATION, e.g. TELEGRAPHIC COMMUNICATION
- H04L9/00—Cryptographic mechanisms or cryptographic arrangements for secret or secure communications; Network security protocols
- H04L9/32—Cryptographic mechanisms or cryptographic arrangements for secret or secure communications; Network security protocols including means for verifying the identity or authority of a user of the system or for message authentication, e.g. authorization, entity authentication, data integrity or data verification, non-repudiation, key authentication or verification of credentials
- H04L9/3226—Cryptographic mechanisms or cryptographic arrangements for secret or secure communications; Network security protocols including means for verifying the identity or authority of a user of the system or for message authentication, e.g. authorization, entity authentication, data integrity or data verification, non-repudiation, key authentication or verification of credentials using a predetermined code, e.g. password, passphrase or PIN
- H04L9/3231—Biological data, e.g. fingerprint, voice or retina
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
Abstract
Description
本出願は、2017年2月22日に出願された米国仮出願第62/462,284の利益を主張し、参照によってその全体が本明細書に組込まれる。
<配列表>
本出願は、ASCIIフォーマットで電子的に提出された配列表を含み、その全体が引用によって本明細書に組み込まれる。2018年2月20日に作成された上記のASCIIコピーは、44854−738_601_SL.txtのファイル名であり、8,636バイトの大きさである。
本明細書で言及される全ての刊行物、特許、および特許出願は、あたかも個々の刊行物、特許、または特許出願が参照により組み込まれるように具体的かつ個々に指示される程度に、参照により本明細書に組み込まれる。
98℃、30秒
98℃、10秒;63℃、10秒;72℃、10秒;12サイクル繰り返す
72℃、2分
Claims (34)
- 情報を保存するための方法であって、該方法は:
a)少なくとも1つのデジタル配列の形態の情報の少なくとも1つのアイテムを受け取る工程と;
b)少なくとも1つのバイオエンクリプションの形式の選択のための指示を受け取る工程であって、ここで、バイオエンクリプションの形式が酵素ベース、電磁気ベース、化学ベース、または親和性ベースのバイオエンクリプションである、工程と;
c)選択されたバイオエンクリプションの形式に基づいて、該少なくとも1つのデジタル配列を複数のオリゴヌクレオチド配列に変換する工程と;
d)該複数のオリゴヌクレオチド配列をコード化する複数のオリゴヌクレオチドを合成する工程と;
e)該複数のオリゴヌクレオチドを保存する工程と、
を含む方法。 - 酵素ベースのバイオエンクリプションは、CRISPR/Casベースのバイオエンクリプションを含む、請求項1に記載の方法。
- 酵素ベースのバイオエンクリプションは、表1に示されるような酵素に感受性があるオリゴヌクレオチドの合成のための指示を含む、請求項1に記載の方法。
- 電磁気ベースのバイオエンクリプションは、約0.01nm〜約400nmの電磁気の波長に感受性があるオリゴヌクレオチドの合成のための指示を含む、請求項1に記載の方法。
- 化学ベースのバイオエンクリプションは、気体のアンモニアまたはメチルアミンの投与に感受性があるオリゴヌクレオチドの合成のための指示を含む、請求項1に記載の方法。
- 親和性ベースのバイオエンクリプションは、配列タグまたは親和性タグに感受性があるオリゴヌクレオチドの合成のための指示を含む、請求項1に記載の方法。
- 親和性タグは、ビオチン、ジゴキシゲニン、Ni−ニトリロ三酢酸、脱硫ビオチン、ヒスチジン、ポリヒスチジン、myc、赤血球凝集素(HA)、FLAG、蛍光タグ、タンデムアフィニティー精製(TAP)タグ、グルタチオンSトランスフェラーゼ(GST)、ポリヌクレオチド、アプタマー、抗原、または抗体である、請求項6に記載の方法。
- 2、3、4、または5つのバイオエンクリプションの形式が使用される、請求項1に記載の方法。
- 複数のオリゴヌクレオチドが少なくとも100,000のオリゴヌクレオチドを含む、請求項1に記載の方法。
- 複数のオリゴヌクレオチドが少なくとも100億のオリゴヌクレオチドを含む、請求項1に記載の方法。
- 情報を検索するための方法であって、該方法は:
a)表面から複数のオリゴヌクレオチドを放出する工程と;
b)酵素ベース、電磁気ベース、化学ベース、または親和性ベースのデクリプションを該複数のオリゴヌクレオチドに適用する工程と;
c)該複数のオリゴヌクレオチドを濃縮する工程と;
d)核酸配列を生成するために、複該数のオリゴヌクレオチドから濃縮したオリゴヌクレオチドを配列決定する工程と;
e)該核酸配列を少なくとも1つのデジタル配列に変換する工程であって、ここで、該少なくとも1つのデジタル配列は情報の少なくとも1つのアイテムをコード化する、工程と、
を含む、方法。 - 複数のオリゴヌクレオチドのデクリプションは、CRISPR/Cas複合体を複数のオリゴヌクレオチドに適用することを含む、請求項11に記載の方法。
- 酵素ベースのデクリプションは、表1に示される酵素を適用することを含む、請求項11に記載の方法。
- 電磁気ベースのデクリプションは、約0.01nm〜約400nmの波長を適用することを含む、請求項11に記載の方法。
- 化学ベースのデクリプションは、気体のアンモニアまたはメチルアミンの投与を適用することを含む、請求項11に記載の方法。
- 親和性ベースのデクリプションは、配列タグまたは親和性タグを適用することを含む、請求項11に記載の方法。
- 親和性タグは、ビオチン、ジゴキシゲニン、Ni−ニトリロ三酢酸、脱硫ビオチン、ヒスチジン、ポリヒスチジン、myc、赤血球凝集素(HA)、FLAG、蛍光タグ、タンデムアフィニティー精製(TAP)タグ、グルタチオンSトランスフェラーゼ(GST)、ポリヌクレオチド、アプタマー、抗原、または抗体である、請求項16に記載の方法。
- 2、3、4、または5つのデクリプションの形態が使用される、請求項11に記載の方法。
- 情報を保存するためのシステムであって、該システムは:
a)少なくとも1つのデジタル配列の形態の情報の少なくとも1つのアイテムのための機械命令、および少なくとも1つのバイオエンクリプションの形式の選択のための機械命令を受け取る受信ユニットであって、ここで、バイオエンクリプションの形式は、酵素ベース、電磁気ベース、化学ベース、または親和性ベースのバイオエンクリプションである、受信ユニットと;
b)選択されたバイオエンクリプションの形式に基づいて、該少なくとも1つのデジタル配列を複数のオリゴヌクレオチド配列に自動的に変換するためのプロセッサーユニットと;
c)該複数のオリゴヌクレオチド配列をコード化する複数のオリゴヌクレオチドを合成するために、該プロセッサーユニットから機械命令を受け取るためのシンセサイザーユニットと;
d)該シンセサイザーユニットから堆積した複数のオリゴヌクレオチドを受け取るためのストレージユニットと、
を含む、システム。 - 酵素ベースのバイオエンクリプションは、CRISPR/Casベースのバイオエンクリプションを含む、請求項19に記載のシステム。
- 酵素ベースのバイオエンクリプションは、表1に示されるような酵素に感受性がある、オリゴヌクレオチドの合成のための機械命令を含む、請求項19に記載のシステム。
- 電磁気ベースのバイオエンクリプションは、約0.01nm〜約400nmの電磁気の波長に感受性があるオリゴヌクレオチドの合成のための機械命令を含む、請求項19に記載のシステム。
- 化学ベースのバイオエンクリプションは、気体のアンモニアまたはメチルアミンの投与に感受性があるオリゴヌクレオチドの合成のための機械命令を含む、請求項19に記載のシステム。
- 親和性ベースのバイオエンクリプションは、配列タグまたは親和性タグに感受性があるオリゴヌクレオチドの合成のための指示を含む、請求項19に記載のシステム。
- 親和性タグは、ビオチン、ジゴキシゲニン、Ni−ニトリロ三酢酸、脱硫ビオチン、ヒスチジン、ポリヒスチジン、myc、赤血球凝集素(HA)、FLAG、蛍光タグ、タンデムアフィニティー精製(TAP)タグ、グルタチオンSトランスフェラーゼ(GST)、ポリヌクレオチド、アプタマー、抗原、または抗体である、請求項24に記載のシステム。
- 複数のオリゴヌクレオチドが少なくとも100,000のオリゴヌクレオチドを含む、請求項24に記載のシステム。
- 複数のオリゴヌクレオチドが少なくとも100億のオリゴヌクレオチドを含む、請求項24に記載のシステム。
- 情報を検索するためのシステムであって、該システムは:
a)表面上の複数のオリゴヌクレオチドを含むストレージユニットと;
b)酵素ベース、電磁気ベース、化学ベース、または親和性ベースのバイオエンクリプションを該複数のオリゴヌクレオチドに適用するためのデポジション装置と;
c)核酸配列を得るために、該複数のオリゴヌクレオチドを配列決定するための配列決定ユニットと;
d)該核酸配列を少なくとも1つのデジタル配列に自動的に変換するためのプロセッサーユニットであって、ここで、該少なくとも1つのデジタル配列は情報の少なくとも1つのアイテムをコード化する、プロセッサーユニットと、
を含む、システム。 - デポジション装置はCRISPR/Cas複合体を複数のオリゴヌクレオチドに適用する、請求項28に記載のシステム。
- 酵素ベースのバイオエンクリプションは、表1に示されるような酵素を適用することを含む、請求項28に記載のシステム。
- 電磁気ベースのバイオエンクリプションは、約0.01nm〜約400nmの波長を適用する、請求項28に記載のシステム。
- 化学ベースのバイオエンクリプションは、気体のアンモニアまたはメチルアミンの投与を適用することを含む、請求項28に記載のシステム。
- 親和性ベースのバイオエンクリプションは、配列タグまたは親和性タグを含む、請求項28に記載のシステム。
- 親和性タグは、ビオチン、ジゴキシゲニン、Ni−ニトリロ三酢酸、脱硫ビオチン、ヒスチジン、ポリヒスチジン、myc、赤血球凝集素(HA)、FLAG、蛍光タグ、タンデムアフィニティー精製(TAP)タグ、グルタチオンSトランスフェラーゼ(GST)、ポリヌクレオチド、アプタマー、抗原、または抗体である、請求項33に記載のシステム。
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SG11201907713WA (en) | 2019-09-27 |
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