ATE246702T1 - Verfahren zur darstellung von oligonukleotiden - Google Patents

Verfahren zur darstellung von oligonukleotiden

Info

Publication number
ATE246702T1
ATE246702T1 AT94914046T AT94914046T ATE246702T1 AT E246702 T1 ATE246702 T1 AT E246702T1 AT 94914046 T AT94914046 T AT 94914046T AT 94914046 T AT94914046 T AT 94914046T AT E246702 T1 ATE246702 T1 AT E246702T1
Authority
AT
Austria
Prior art keywords
image
oligonucleotides
shown below
preparing oligonucleotides
reaction
Prior art date
Application number
AT94914046T
Other languages
English (en)
Inventor
Robert L Letsinger
Sergei M Gryaznov
Original Assignee
Univ Northwestern
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Northwestern filed Critical Univ Northwestern
Application granted granted Critical
Publication of ATE246702T1 publication Critical patent/ATE246702T1/de

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12Antivirals
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Oncology (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT94914046T 1993-04-12 1994-04-06 Verfahren zur darstellung von oligonukleotiden ATE246702T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4603293A 1993-04-12 1993-04-12
PCT/US1994/003747 WO1994024143A1 (en) 1993-04-12 1994-04-06 Method of forming oligonucleotides

Publications (1)

Publication Number Publication Date
ATE246702T1 true ATE246702T1 (de) 2003-08-15

Family

ID=21941212

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94914046T ATE246702T1 (de) 1993-04-12 1994-04-06 Verfahren zur darstellung von oligonukleotiden

Country Status (9)

Country Link
US (1) US5476930A (de)
EP (1) EP0695305B1 (de)
JP (2) JP4079875B2 (de)
AT (1) ATE246702T1 (de)
AU (1) AU684279B2 (de)
CA (1) CA2160016C (de)
DE (1) DE69433010T2 (de)
ES (1) ES2204913T3 (de)
WO (1) WO1994024143A1 (de)

Families Citing this family (151)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09500378A (ja) * 1993-07-02 1997-01-14 リンクス セラピューティクス,インコーポレイティド 枝分れされそして複雑に結合された高分子構造体の集中的合成
US5830658A (en) * 1995-05-31 1998-11-03 Lynx Therapeutics, Inc. Convergent synthesis of branched and multiply connected macromolecular structures
US5888731A (en) * 1995-08-30 1999-03-30 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes
US6335437B1 (en) * 1998-09-07 2002-01-01 Isis Pharmaceuticals, Inc. Methods for the preparation of conjugated oligomers
US7033753B1 (en) 1999-01-15 2006-04-25 University Of Rochester Compositions and methods for nonenzymatic ligation of oligonucleotides and detection of genetic polymorphisms
AU2001265121A1 (en) * 2000-05-30 2001-12-11 Applera Corporation Methods for detecting target nucleic acids using coupled ligation and amplification
EP1317464B1 (de) * 2000-10-11 2009-05-13 Applera Corporation Fluoreszierende nukleobasekonjugate mit anionische linker
US6951720B2 (en) * 2001-05-10 2005-10-04 San Diego State University Foundation Use of phosphorothiolate polynucleotides in ligating nucleic acids
WO2002102820A1 (en) 2001-06-20 2002-12-27 Nuevolution A/S Nucleoside derivatives for library preparation
DE10142643A1 (de) * 2001-08-31 2003-04-24 Clondiag Chip Tech Gmbh Detektion von Wechselwirkungen auf Sonden-Arrays
DE10147074A1 (de) * 2001-09-25 2003-05-08 Beru Ag Verfahren zum Betreiben einer aus mehreren Heizelementen bestehenden mehrstufigen elektrischen Heizung
JP2005517900A (ja) 2001-11-21 2005-06-16 アプレラ コーポレイション デジタルアッセイ
DE20200926U1 (de) * 2002-01-23 2002-04-18 Hegenscheidt-MFD GmbH & Co. KG, 41812 Erkelenz Festwalzgerät einer Festwalzmaschine für Kurbelwellen
AU2003253069A1 (en) * 2002-03-15 2003-09-29 Nuevolution A/S A building block forming a c-c bond upon reaction
WO2003078626A2 (en) * 2002-03-15 2003-09-25 Nuevolution A/S A building block capable of transferring a functional entity
WO2003078625A2 (en) 2002-03-15 2003-09-25 Nuevolution A/S An improved method for synthesising templated molecules
WO2003078446A2 (en) * 2002-03-15 2003-09-25 Nuevolution A/S A building block forming a c-c or a c-hetero atom bond upon reaction
EP1543156A4 (de) * 2002-07-18 2005-12-21 Univ Leland Stanford Junior Nachweis chemischer ligation unter verwendung von fluoreszenzquencher-abgangsgruppen
EP1539980B1 (de) 2002-08-01 2016-02-17 Nuevolution A/S MEHRSTUFENSYNTHESE VON MIT MATRIZEN ASSOZIIERTE MOLEKüLEN
WO2004039825A2 (en) 2002-10-30 2004-05-13 Nuevolution A/S Method for the synthesis of a bifunctional complex
US20050053957A1 (en) * 2002-11-19 2005-03-10 Applera Corporation Polynucleotide sequence detection assays
WO2004046343A2 (en) * 2002-11-19 2004-06-03 Applera Corporation Polynucleotide sequence detection assays and analysis
WO2004056994A2 (en) 2002-12-19 2004-07-08 Nuevolution A/S Quasirandom structure and function guided synthesis methods
WO2004074429A2 (en) * 2003-02-21 2004-09-02 Nuevolution A/S Method for producing second-generation library
GB0304371D0 (en) * 2003-02-26 2003-04-02 Solexa Ltd DNA Sequence analysis
US7915201B2 (en) 2003-03-20 2011-03-29 Nuevolution A/S Ligational encoding of small molecules
WO2005001129A2 (en) * 2003-06-06 2005-01-06 Applera Corporation Mobility cassettes
CN100412332C (zh) * 2003-07-10 2008-08-20 雅马哈发动机株式会社 骑乘型车辆用发动机
ATE447626T1 (de) 2003-09-18 2009-11-15 Nuevolution As Methode zur gewinnung struktureller informationen kodierter moleküle und zur selektion von verbindungen
DK1730277T3 (da) 2004-03-22 2010-03-01 Nuevolution As Kodning ved ligering under anvendelse af byggebloksoligonukleotider
WO2006112818A2 (en) 2005-04-14 2006-10-26 Applera Corporation 3' modified oligonucleotides containing pseudoisocytosine nucleobase derivatives and applications thereof as primers or probes
ES2502815T3 (es) 2004-10-27 2014-10-06 Cepheid Reacciones de amplificación de ácido nucleico de múltiples estadios en sistema cerrado
WO2006076650A2 (en) * 2005-01-12 2006-07-20 Applera Corporation Compositions, methods, and kits for selective amplification of nucleic acids
US20060160102A1 (en) 2005-01-18 2006-07-20 Hossein Fakhrai-Rad Identification of rare alleles by enzymatic enrichment of mismatched heteroduplexes
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
DK1907571T3 (en) 2005-06-15 2017-08-21 Complete Genomics Inc NUCLEIC ACID ANALYSIS USING INCIDENTAL MIXTURES OF NON-OVERLAPPING FRAGMENTS
US20070087360A1 (en) * 2005-06-20 2007-04-19 Boyd Victoria L Methods and compositions for detecting nucleotides
CA2624896C (en) 2005-10-07 2017-11-07 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
SI3305900T1 (sl) 2005-12-01 2022-02-28 Nuevolution A/S Postopki encimskega kodiranja za učinkovito sintezo velikih knjižnic
US8900828B2 (en) 2006-05-01 2014-12-02 Cepheid Methods and apparatus for sequential amplification reactions
EP2021514A2 (de) 2006-05-10 2009-02-11 Dxterity Diagnostics Erkennung von nukleinsäuretargets mithilfe chemisch reaktiver oligonnucleotid-sonden
US8119352B2 (en) * 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
US20090036325A1 (en) * 2007-05-25 2009-02-05 Applera Corporation Directed assembly of amplicons to enhance read pairing signature with massively parallel short read sequencers
US8008010B1 (en) 2007-06-27 2011-08-30 Applied Biosystems, Llc Chimeric oligonucleotides for ligation-enhanced nucleic acid detection, methods and compositions therefor
EP2395113A1 (de) 2007-06-29 2011-12-14 Population Genetics Technologies Ltd. Verfahren und Zusammensetzungen zur Isolierung von Nukleinsäuresequenzvarianten
DK2215209T3 (en) * 2007-10-30 2018-09-03 Complete Genomics Inc DEVICE FOR HIGH-THROUGHPUT SEQUENCE OF NUCLEIC ACIDS
US9551026B2 (en) 2007-12-03 2017-01-24 Complete Genomincs, Inc. Method for nucleic acid detection using voltage enhancement
AP3396A (en) 2009-02-13 2015-08-31 Chem Inc X Methods of creating and screening DNA-encoded libraries
EP2765205B1 (de) * 2009-04-01 2016-05-18 DxTerity Diagnostics Incorporated Chemische ligationsabhängige Sondenamplifizierung (CLPA)
WO2010133972A1 (en) 2009-05-22 2010-11-25 Population Genetics Technologies Ltd Sorting asymmetrically tagged nucleic acids by selective primer extension
WO2011021102A2 (en) 2009-08-20 2011-02-24 Population Genetics Technologies Ltd Compositions and methods for intramolecular nucleic acid rearrangement
US20120245041A1 (en) 2009-11-04 2012-09-27 Sydney Brenner Base-by-base mutation screening
EP2504448B1 (de) 2009-11-25 2016-10-19 Bio-Rad Laboratories, Inc. Verfahren und zusammensetzung zur erkennung von genetischem material
WO2011101744A2 (en) 2010-02-22 2011-08-25 Population Genetics Technologies Ltd. Region of interest extraction and normalization methods
WO2011107887A2 (en) 2010-03-02 2011-09-09 Population Genetic Technologies Ltd. Methods for replicating polynucleotides with secondary structure
EP3540059A1 (de) 2010-04-16 2019-09-18 Nuevolution A/S Bifunktionelle komplexe und verfahren zur herstellung sowie verwendung derartiger komplexe
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
EP2585593B1 (de) 2010-06-24 2017-03-01 Population Genetics Technologies Ltd. Verfahren zur herstellung und verewigung einer polynukleotid-bibliothek sowie zur extraktion relevanter bereiche daraus
ES2595433T3 (es) 2010-09-21 2016-12-30 Population Genetics Technologies Ltd. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
US8725422B2 (en) 2010-10-13 2014-05-13 Complete Genomics, Inc. Methods for estimating genome-wide copy number variations
EP3564392B1 (de) 2010-12-17 2021-11-24 Life Technologies Corporation Verfahren für nukleinsäureamplifikation
WO2013082164A1 (en) 2011-11-28 2013-06-06 Life Technologies Corporation Enhanced ligation reactions
US20120196294A1 (en) 2011-01-17 2012-08-02 Life Technologies Corporation Workflow for detection of ligands using nucleic acids
EP3733867A1 (de) 2011-01-17 2020-11-04 Life Technologies Corporation Enzymatische ligation von nukleinsäuren
WO2012108864A1 (en) 2011-02-08 2012-08-16 Illumina, Inc. Selective enrichment of nucleic acids
EP3483285B1 (de) 2011-02-09 2021-07-14 Bio-Rad Laboratories, Inc. Analyse von nukleinsäuren
CN106434871B (zh) 2011-05-17 2020-08-18 德克斯特里蒂诊断公司 用于检测目标核酸的方法与组合物
DK2729580T3 (en) 2011-07-08 2015-12-14 Keygene Nv SEQUENCE BASED genotyping BASED ON OLIGONUKLEOTIDLIGERINGSASSAYS
EP2748357B1 (de) 2011-09-07 2018-04-04 X-Chem, Inc. Verfahren zur markierung dna-kodierter bibliotheken
US10501791B2 (en) 2011-10-14 2019-12-10 President And Fellows Of Harvard College Sequencing by structure assembly
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
EP2798086B1 (de) * 2011-12-28 2018-09-05 Ricardo Mancebo Reagenzien und verfahren für eine autoligationskettenreaktion
WO2013123125A1 (en) 2012-02-17 2013-08-22 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
WO2013124743A1 (en) 2012-02-22 2013-08-29 Population Genetics Technologies Ltd. Compositions and methods for intramolecular nucleic acid rearrangement ii
ES2741099T3 (es) 2012-02-28 2020-02-10 Agilent Technologies Inc Método de fijación de una secuencia de recuento para una muestra de ácido nucleico
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
US9628676B2 (en) 2012-06-07 2017-04-18 Complete Genomics, Inc. Imaging systems with movable scan mirrors
US9488823B2 (en) 2012-06-07 2016-11-08 Complete Genomics, Inc. Techniques for scanned illumination
GB2518078B (en) 2012-06-18 2015-04-29 Nugen Technologies Inc Compositions and methods for negative selection of non-desired nucleic acid sequences
SG10201907347YA (en) 2012-07-13 2019-09-27 X Chem Inc Dna-encoded libraries having encoding oligonucleotide linkages not readable by polymerases
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
ES2637538T3 (es) 2012-07-17 2017-10-13 Counsyl, Inc. Sistema y métodos para la detección de una variación genética
WO2014121091A1 (en) 2013-02-01 2014-08-07 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
JP6441893B2 (ja) 2013-03-19 2018-12-19 ディレクティド・ジェノミクス・エル・エル・シー 標的配列の濃縮
EP2986741B1 (de) 2013-04-17 2019-07-24 Agency For Science, Technology And Research Verfahren zur erzeugung erweiterter sequenzauslesungen
TWI805996B (zh) 2013-08-05 2023-06-21 美商扭轉生物科技有限公司 重新合成之基因庫
EP3628747B1 (de) 2013-12-05 2022-10-05 Centrillion Technology Holdings Corporation Herstellung von gemusterten anordnungen
EP3077545B1 (de) 2013-12-05 2020-09-16 Centrillion Technology Holdings Corporation Verfahren zur sequenzierung von nukleinsäuren
CN105940024B (zh) 2013-12-05 2019-03-15 生捷科技控股公司 修饰的表面
EP3540074A1 (de) 2013-12-11 2019-09-18 The Regents of the University of California Verfahren zur markierung innerer regionen von nukleinsäuremolekülen
US11060139B2 (en) 2014-03-28 2021-07-13 Centrillion Technology Holdings Corporation Methods for sequencing nucleic acids
CA2951561C (en) 2014-06-10 2023-04-18 Dxterity Diagnostics Incorporated Devices and methods for collecting and stabilizing biological samples
SG11201702066UA (en) 2014-07-21 2017-04-27 Illumina Inc Polynucleotide enrichment using crispr-cas systems
US10526641B2 (en) 2014-08-01 2020-01-07 Dovetail Genomics, Llc Tagging nucleic acids for sequence assembly
AU2015346514B2 (en) 2014-11-11 2021-04-08 Illumina, Inc. Polynucleotide amplification using CRISPR-Cas systems
US10233490B2 (en) 2014-11-21 2019-03-19 Metabiotech Corporation Methods for assembling and reading nucleic acid sequences from mixed populations
WO2016093838A1 (en) 2014-12-11 2016-06-16 New England Biolabs, Inc. Enrichment of target sequences
WO2016126987A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
NZ734854A (en) 2015-02-17 2022-11-25 Dovetail Genomics Llc Nucleic acid sequence assembly
WO2016154540A1 (en) 2015-03-26 2016-09-29 Dovetail Genomics Llc Physical linkage preservation in dna storage
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
EP3527672B1 (de) 2015-06-09 2022-10-05 Centrillion Technology Holdings Corporation Oligonukleotide arrays zur sequenzierung von nukleinsäuren
HK1258869A1 (zh) 2015-09-18 2019-11-22 Twist Bioscience Corporation 寡核酸变体文库及其合成
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
AU2016341198B2 (en) 2015-10-19 2023-03-09 Dovetail Genomics, Llc Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection
CN108603307A (zh) 2015-12-01 2018-09-28 特韦斯特生物科学公司 功能化表面及其制备
CA3014911A1 (en) 2016-02-23 2017-08-31 Dovetail Genomics, Llc Generation of phased read-sets for genome assembly and haplotype phasing
CN109477137B (zh) 2016-05-11 2023-05-30 伊鲁米那股份有限公司 使用argonaute系统的多核苷酸富集和扩增
DK3455356T3 (da) 2016-05-13 2021-11-01 Dovetail Genomics Llc Genfinding af langtrækkende bindingsinformation fra konserverede prøver
US11708574B2 (en) 2016-06-10 2023-07-25 Myriad Women's Health, Inc. Nucleic acid sequencing adapters and uses thereof
WO2018005811A1 (en) 2016-06-30 2018-01-04 Grail, Inc. Differential tagging of rna for preparation of a cell-free dna/rna sequencing library
WO2018038772A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
EP3516528B1 (de) 2016-09-21 2025-10-15 Atlas Data Storage, Inc. Nukleinsäurebasierte datenspeicherung
WO2018064486A1 (en) 2016-09-29 2018-04-05 Counsyl, Inc. Noninvasive prenatal screening using dynamic iterative depth optimization
WO2018112426A1 (en) 2016-12-16 2018-06-21 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018136856A1 (en) 2017-01-23 2018-07-26 Massachusetts Institute Of Technology Multiplexed signal amplified fish via splinted ligation amplification and sequencing
WO2018144217A1 (en) 2017-01-31 2018-08-09 Counsyl, Inc. Methods and compositions for enrichment of target polynucleotides
WO2018144216A1 (en) 2017-01-31 2018-08-09 Counsyl, Inc. Methods and compositions for enrichment of target polynucleotides
EP3580337A4 (de) 2017-02-10 2020-12-02 Zymergen, Inc. Modulare universelle plasmiddesignstrategie für die zusammenstellung und bearbeitung von mehreren dna-konstrukten für mehrere wirte
KR20240158370A (ko) 2017-02-22 2024-11-04 트위스트 바이오사이언스 코포레이션 핵산 기반 데이터 저장
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
EP3602359A4 (de) 2017-03-24 2021-01-06 Myriad Women's Health, Inc. Kopienzahlvariantencaller
CA3060539A1 (en) 2017-04-18 2018-10-25 Dovetail Genomics, Llc Nucleic acid characteristics as guides for sequence assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
KR102628876B1 (ko) 2017-06-12 2024-01-23 트위스트 바이오사이언스 코포레이션 심리스 핵산 어셈블리를 위한 방법
CA3066745A1 (en) 2017-06-26 2019-01-03 Phase Genomics Inc. A method for the clustering of dna sequences
US11180804B2 (en) 2017-07-25 2021-11-23 Massachusetts Institute Of Technology In situ ATAC sequencing
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
US10894242B2 (en) 2017-10-20 2021-01-19 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
EP3735459B1 (de) 2018-01-04 2026-03-04 Atlas Data Storage, Inc. Digitale informationsspeicherung auf dna-basis
US12378592B2 (en) 2018-01-31 2025-08-05 Dovetail Genomics, Llc. Sample prep for DNA linkage recovery
SG11202011467RA (en) 2018-05-18 2020-12-30 Twist Bioscience Corp Polynucleotides, reagents, and methods for nucleic acid hybridization
WO2020086144A2 (en) 2018-08-15 2020-04-30 Zymergen Inc. APPLICATIONS OF CRISPRi IN HIGH THROUGHPUT METABOLIC ENGINEERING
CN113692409B (zh) 2018-12-26 2025-01-10 特韦斯特生物科学公司 高度准确的从头多核苷酸合成
EP3930753A4 (de) 2019-02-26 2023-03-29 Twist Bioscience Corporation Variante nukleinsäurebibliotheken für glp1-rezeptor
US11492728B2 (en) 2019-02-26 2022-11-08 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
CA3144644A1 (en) 2019-06-21 2020-12-24 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
US20220267826A1 (en) 2019-06-27 2022-08-25 Dovetail Genomics, Llc Methods and compositions for proximity ligation
CN115023440B (zh) 2019-09-23 2025-09-12 特韦斯特生物科学公司 结合CD3ε的抗体
EP4464827A3 (de) 2019-09-23 2025-02-26 Twist Bioscience Corporation Varianten von nukleinsäurebibliotheken für crth2
KR20220088632A (ko) 2019-10-25 2022-06-28 일루미나 케임브리지 리미티드 헤어핀 루프를 포함하는 폴리뉴클레오티드 주형의 말단에 비대칭 어댑터를 생성하고 이로부터 서열분석하는 방법
US12570750B2 (en) 2019-12-09 2026-03-10 Twist Bioscience Corporation Antibodies that bind adenosine A2A receptors and methods of use thereof to treat cancer and neurological diseases
US20240287504A1 (en) 2021-03-09 2024-08-29 Illumina, Inc. Genomic library preparation and targeted epigenetic assays using cas-grna ribonucleoproteins
WO2022192191A1 (en) 2021-03-09 2022-09-15 Illumina, Inc. Analyzing expression of protein-coding variants in cells
US20240158839A1 (en) 2021-03-15 2024-05-16 President And Fellows Of Harvard College Methods for in situ sequencing
WO2023035003A1 (en) 2021-09-03 2023-03-09 Elegen Corp. Multi-way bead-sorting devices, systems, and methods of use thereof using pressure sources
US20230392201A1 (en) 2022-06-06 2023-12-07 Element Biosciences, Inc. Methods for assembling and reading nucleic acid sequences from mixed populations
WO2024015766A1 (en) 2022-07-12 2024-01-18 Topogene Inc. Scalable, submicron-resolution replication of dna arrays
WO2025019313A1 (en) 2023-07-14 2025-01-23 The Broad Institute, Inc. Method for assembling gene libraries from a pool of oligonucleotides

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5237016A (en) * 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids

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DE69433010D1 (de) 2003-09-11
EP0695305B1 (de) 2003-08-06
AU684279B2 (en) 1997-12-11
JPH08508998A (ja) 1996-09-24
JP4079875B2 (ja) 2008-04-23
JP2005015490A (ja) 2005-01-20
JP3593344B2 (ja) 2004-11-24
DE69433010T2 (de) 2004-06-09
EP0695305A4 (de) 1996-12-11
CA2160016C (en) 2008-06-03
ES2204913T3 (es) 2004-05-01
EP0695305A1 (de) 1996-02-07
CA2160016A1 (en) 1994-10-27
AU6626694A (en) 1994-11-08
US5476930A (en) 1995-12-19
WO1994024143A1 (en) 1994-10-27
JP2004129672A (ja) 2004-04-30

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