JPH08508998A - オリゴヌクレオチドの形成方法 - Google Patents
オリゴヌクレオチドの形成方法Info
- Publication number
- JPH08508998A JPH08508998A JP6523287A JP52328794A JPH08508998A JP H08508998 A JPH08508998 A JP H08508998A JP 6523287 A JP6523287 A JP 6523287A JP 52328794 A JP52328794 A JP 52328794A JP H08508998 A JPH08508998 A JP H08508998A
- Authority
- JP
- Japan
- Prior art keywords
- group
- oligonucleotides
- oligonucleotide
- forming
- reactive group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.a)オリゴマーの塩基単位に相補的な塩基単位を含むオリゴ−又はポリヌク レオチド上の適切な位置に、少なくとも2つのオリゴヌクレオチドを可逆的に結 合する工程であって、オリゴヌクレオチドのうち1つは、第1の反応基を有する ヌクレオチドを含み、かつ第2の反応基を含む他のオリゴマーのヌクレオチドと 近接し、第2の反応基が第1の反応基と共有結合を自発的に形成する工程と、 b)ポリヌクレオチド上のオリゴヌクレオチドの結合においてお互いに近接 する第1及び第2の反応基を介してオリゴヌクレオチドを不可逆的に共有結合す る工程とによるオリゴヌクレオチドの形成方法。 2.前記第1の反応基がα−ハロアシルであり、前記第2の反応基がホスホチオ エートであり、前記工程(b)が前記反応基を通して自発的にチオホスホリルア セチルアミノ結合を形成するものとしてさらに定義される請求項1記載のオリゴ ヌクレオチド形成方法。 3.オリゴマーの各々が8〜20ヌクレオチドからなる請求項1記載のオリゴヌ クレオチド形成方法。 4.前記工程(a)及び(b)が水溶液中で生じる請求項1記載のオリゴヌクレオ チド形成方法。 5.a)オリゴヌクレオチドのうち1つがα−ハロアシル基を含み、他のヌクレ オチドがホスホチオエート基を含む、少なくとも2つのオリゴヌクレオチドを水 溶液に配置する工程と、 b)α−ハロアシル基及びホスホチオエート基を通して、それらの間にチオ ホスホリルアセチルアミノ基を自発的に形成して、共にオリゴヌクレオチドを共 有結合する工程とによるオリゴヌクレオチドの形成方法。 6.オリゴヌクレオチドを含む水溶液を凍結させることにより反応を促進し、高 い完結度で反応を行う工程(c)をさらに含む請求項5記載のオリゴヌクレオチ ド形成方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US4603293A | 1993-04-12 | 1993-04-12 | |
US08/046,032 | 1993-04-12 | ||
PCT/US1994/003747 WO1994024143A1 (en) | 1993-04-12 | 1994-04-06 | Method of forming oligonucleotides |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2003433247A Division JP4079875B2 (ja) | 1993-04-12 | 2003-12-26 | オリゴヌクレオチドの形成方法 |
Publications (3)
Publication Number | Publication Date |
---|---|
JPH08508998A true JPH08508998A (ja) | 1996-09-24 |
JP3593344B2 JP3593344B2 (ja) | 2004-11-24 |
JP3593344B6 JP3593344B6 (ja) | 2005-02-16 |
Family
ID=
Also Published As
Publication number | Publication date |
---|---|
ATE246702T1 (de) | 2003-08-15 |
WO1994024143A1 (en) | 1994-10-27 |
JP4079875B2 (ja) | 2008-04-23 |
JP2005015490A (ja) | 2005-01-20 |
ES2204913T3 (es) | 2004-05-01 |
CA2160016A1 (en) | 1994-10-27 |
AU6626694A (en) | 1994-11-08 |
AU684279B2 (en) | 1997-12-11 |
US5476930A (en) | 1995-12-19 |
EP0695305A4 (en) | 1996-12-11 |
DE69433010D1 (de) | 2003-09-11 |
EP0695305A1 (en) | 1996-02-07 |
DE69433010T2 (de) | 2004-06-09 |
EP0695305B1 (en) | 2003-08-06 |
CA2160016C (en) | 2008-06-03 |
JP3593344B2 (ja) | 2004-11-24 |
JP2004129672A (ja) | 2004-04-30 |
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