LT3027771T - Ilgos kloninės sekos verifikuotų nukleorūgščių konstruktų gamybos būdas - Google Patents

Ilgos kloninės sekos verifikuotų nukleorūgščių konstruktų gamybos būdas

Info

Publication number
LT3027771T
LT3027771T LTEP14831238.2T LT14831238T LT3027771T LT 3027771 T LT3027771 T LT 3027771T LT 14831238 T LT14831238 T LT 14831238T LT 3027771 T LT3027771 T LT 3027771T
Authority
LT
Lithuania
Prior art keywords
production
methods
nucleic acid
long length
acid constructs
Prior art date
Application number
LTEP14831238.2T
Other languages
English (en)
Inventor
Joseph Jacobson
Michael E. Hudson
Li-Yun Kung
Original Assignee
Gen9, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen9, Inc. filed Critical Gen9, Inc.
Publication of LT3027771T publication Critical patent/LT3027771T/lt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
LTEP14831238.2T 2013-07-30 2014-07-30 Ilgos kloninės sekos verifikuotų nukleorūgščių konstruktų gamybos būdas LT3027771T (lt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361859946P 2013-07-30 2013-07-30
US201361909526P 2013-11-27 2013-11-27
PCT/US2014/048867 WO2015017527A2 (en) 2013-07-30 2014-07-30 Methods for the production of long length clonal sequence verified nucleic acid constructs

Publications (1)

Publication Number Publication Date
LT3027771T true LT3027771T (lt) 2019-04-25

Family

ID=52432564

Family Applications (1)

Application Number Title Priority Date Filing Date
LTEP14831238.2T LT3027771T (lt) 2013-07-30 2014-07-30 Ilgos kloninės sekos verifikuotų nukleorūgščių konstruktų gamybos būdas

Country Status (4)

Country Link
US (2) US20160168564A1 (lt)
EP (1) EP3027771B1 (lt)
LT (1) LT3027771T (lt)
WO (1) WO2015017527A2 (lt)

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ES2737957T3 (es) 2011-08-26 2020-01-17 Gen9 Inc Composiciones y métodos para el ensamblaje de alta fidelidad de ácidos nucleicos
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CN104603286B (zh) 2012-04-24 2020-07-31 Gen9股份有限公司 在体外克隆中分选核酸和多重制备物的方法
CN113512577A (zh) 2012-06-25 2021-10-19 Gen9股份有限公司 用于核酸组装和高通量测序的方法
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CA2975855A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
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CN113604546A (zh) 2015-09-22 2021-11-05 特韦斯特生物科学公司 用于核酸合成的柔性基底
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CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
KR20240013290A (ko) 2017-06-12 2024-01-30 트위스트 바이오사이언스 코포레이션 심리스 핵산 어셈블리를 위한 방법
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
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CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
EP3735459A4 (en) 2018-01-04 2021-10-06 Twist Bioscience Corporation DNA-BASED DIGITAL INFORMATION STORAGE
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CN114729342A (zh) 2019-06-21 2022-07-08 特韦斯特生物科学公司 基于条形码的核酸序列装配

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Also Published As

Publication number Publication date
EP3027771A2 (en) 2016-06-08
EP3027771A4 (en) 2017-03-01
WO2015017527A3 (en) 2015-10-29
US20220333096A1 (en) 2022-10-20
EP3027771B1 (en) 2019-01-16
WO2015017527A2 (en) 2015-02-05
US20160168564A1 (en) 2016-06-16

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