JP2013524171A5 - - Google Patents

Download PDF

Info

Publication number
JP2013524171A5
JP2013524171A5 JP2013501537A JP2013501537A JP2013524171A5 JP 2013524171 A5 JP2013524171 A5 JP 2013524171A5 JP 2013501537 A JP2013501537 A JP 2013501537A JP 2013501537 A JP2013501537 A JP 2013501537A JP 2013524171 A5 JP2013524171 A5 JP 2013524171A5
Authority
JP
Japan
Prior art keywords
channel
droplet
sample
well
fluid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2013501537A
Other languages
English (en)
Japanese (ja)
Other versions
JP2013524171A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2011/030101 external-priority patent/WO2011120024A1/en
Publication of JP2013524171A publication Critical patent/JP2013524171A/ja
Publication of JP2013524171A5 publication Critical patent/JP2013524171A5/ja
Pending legal-status Critical Current

Links

JP2013501537A 2010-03-25 2011-03-25 液滴ベースのアッセイのための液滴の発生 Pending JP2013524171A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US34121810P 2010-03-25 2010-03-25
US61/341,218 2010-03-25
PCT/US2011/030101 WO2011120024A1 (en) 2010-03-25 2011-03-25 Droplet generation for droplet-based assays

Publications (2)

Publication Number Publication Date
JP2013524171A JP2013524171A (ja) 2013-06-17
JP2013524171A5 true JP2013524171A5 (enExample) 2014-05-15

Family

ID=44673670

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2013501537A Pending JP2013524171A (ja) 2010-03-25 2011-03-25 液滴ベースのアッセイのための液滴の発生

Country Status (5)

Country Link
US (6) US9500664B2 (enExample)
EP (1) EP2550528B1 (enExample)
JP (1) JP2013524171A (enExample)
CA (1) CA2767182C (enExample)
WO (1) WO2011120024A1 (enExample)

Families Citing this family (219)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
WO2007081385A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
EP2298438A1 (en) 2006-02-07 2011-03-23 Stokes Bio Limited A microfluidic droplet queuing network
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
WO2007133710A2 (en) 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
JP5738597B2 (ja) 2007-12-21 2015-06-24 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸の配列決定のためのシステムおよび方法
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
GB2502409B8 (en) 2008-09-23 2014-10-15 Bio Rad Laboratories Droplet-based assay system
US9764322B2 (en) * 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9089844B2 (en) 2010-11-01 2015-07-28 Bio-Rad Laboratories, Inc. System for forming emulsions
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US8663920B2 (en) 2011-07-29 2014-03-04 Bio-Rad Laboratories, Inc. Library characterization by digital assay
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US9492797B2 (en) * 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
EP3290531B1 (en) 2008-12-19 2019-07-24 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
KR101793744B1 (ko) 2009-03-13 2017-11-03 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 유동 포커싱 미세유동 장치의 규모 확장
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
CA2767056C (en) 2009-09-02 2018-12-04 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
EP2473263B1 (en) 2009-09-02 2022-11-02 President and Fellows of Harvard College Multiple emulsions created using jetting and other techniques
EP2493619B1 (en) 2009-10-27 2018-12-19 President and Fellows of Harvard College Droplet creation techniques
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
EP4435111A1 (en) 2010-02-12 2024-09-25 Bio-Rad Laboratories, Inc. Digital analyte analysis
JP2013524169A (ja) 2010-03-25 2013-06-17 クァンタライフ・インコーポレーテッド 液滴によるアッセイ用の検出システム
JP2013524171A (ja) 2010-03-25 2013-06-17 クァンタライフ・インコーポレーテッド 液滴ベースのアッセイのための液滴の発生
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
EP3859011A1 (en) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Methods for forming mixed droplets
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
CA2830443C (en) 2011-03-18 2021-11-16 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
JP2014512826A (ja) 2011-04-25 2014-05-29 バイオ−ラド ラボラトリーズ インコーポレイテッド 核酸分析のための方法および組成物
WO2013029631A2 (en) * 2011-08-31 2013-03-07 Koch Joern Erland Enzyme detection by microfluidics
BR112013029729A2 (pt) 2011-05-23 2017-01-24 Basf Se controle de emulsões, incluindo emulsões múltiplas
EP2714970B1 (en) 2011-06-02 2017-04-19 Raindance Technologies, Inc. Enzyme quantification
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
GB201115895D0 (en) 2011-09-14 2011-10-26 Embl Microfluidic device
EP2760578B1 (en) 2011-09-28 2020-08-26 President and Fellows of Harvard College Systems and methods for droplet production and/or fluidic manipulation
US10222391B2 (en) 2011-12-07 2019-03-05 The Johns Hopkins University System and method for screening a library of samples
MX2014013332A (es) 2012-05-04 2015-02-05 Siemens Healthcare Diagnostics Sistema de introduccion de muestras.
FR2991055B1 (fr) 2012-05-22 2014-06-13 C2 Diagnostics Dispositif de connexion fluidique pour appareils d'analyse biologique, composant fluidique adapte et appareil d'analyse biologique ainsi equipe;
WO2014028378A2 (en) 2012-08-13 2014-02-20 The Regents Of The University Of California Methods and systems for detecting biological components
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
CN114891871A (zh) 2012-08-14 2022-08-12 10X基因组学有限公司 微胶囊组合物及方法
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140193857A1 (en) * 2012-09-12 2014-07-10 Cypho, Inc. Centrifuge tube droplet generator
US11118218B2 (en) * 2012-09-12 2021-09-14 Cypho, Inc. Common port emulsion generation system
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR20230003659A (ko) 2013-02-08 2023-01-06 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
WO2014145760A1 (en) * 2013-03-15 2014-09-18 Bio-Rad Laboratories, Inc. Droplet generator with collection tube
EP4566715A3 (en) * 2013-04-02 2025-08-27 Bio-Rad Laboratories, Inc. Systems and methods for handling microfluidic droplets
US9403294B2 (en) * 2013-04-17 2016-08-02 Bio-Rad Laboratories, Inc. Method of making a droplet-generating device
AU2014284148B2 (en) * 2013-06-21 2017-02-23 Bio-Rad Laboratories, Inc. Microfluidic system with fluid pickups
EP2848698A1 (en) * 2013-08-26 2015-03-18 F. Hoffmann-La Roche AG System and method for automated nucleic acid amplification
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
WO2015069634A1 (en) 2013-11-08 2015-05-14 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
WO2015103367A1 (en) * 2013-12-31 2015-07-09 Raindance Technologies, Inc. System and method for detection of rna species
US9694361B2 (en) 2014-04-10 2017-07-04 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
EP4235677A3 (en) 2014-06-26 2023-11-22 10X Genomics, Inc. Processes and systems for nucleic acid sequence assembly
EP4574974A3 (en) 2014-06-26 2025-10-08 10x Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
KR20170026383A (ko) 2014-06-26 2017-03-08 10엑스 제노믹스, 인크. 핵산 서열의 분석
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
EP3160654A4 (en) 2014-06-27 2017-11-15 The Regents of The University of California Pcr-activated sorting (pas)
JP2017532024A (ja) 2014-09-09 2017-11-02 ザ・ブロード・インスティテュート・インコーポレイテッド コンポジット単一細胞核酸分析のための液滴ベースの方法および機器
CN104237186B (zh) * 2014-09-15 2017-01-25 华中科技大学 一种荧光成像装置及方法
US10434507B2 (en) 2014-10-22 2019-10-08 The Regents Of The University Of California High definition microdroplet printer
JP2017532042A (ja) 2014-10-29 2017-11-02 10エックス ゲノミクス,インコーポレイテッド 標的化核酸配列決定のための方法及び組成物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
WO2016078339A1 (zh) * 2014-11-17 2016-05-26 中国科学院微生物研究所 微液滴生成装置、系统、方法及单细胞/单分子分析装置
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
CA2968417A1 (en) 2015-01-13 2016-07-21 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
CN107530654A (zh) 2015-02-04 2018-01-02 加利福尼亚大学董事会 通过在离散实体中条形码化对核酸进行测序
SG11201705996PA (en) 2015-02-09 2017-09-28 10X Genomics Inc Systems and methods for determining structural variation and phasing using variant call data
AU2016222719B2 (en) 2015-02-24 2022-03-31 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
WO2016138175A1 (en) 2015-02-24 2016-09-01 The University Of British Columbia Continuous flow microfluidic system
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
US11873483B2 (en) 2015-03-11 2024-01-16 The Broad Institute, Inc. Proteomic analysis with nucleic acid identifiers
KR102473981B1 (ko) * 2015-03-24 2022-12-05 프리시젼바이오 주식회사 시료 검사 장치
CN107921381B (zh) 2015-04-28 2021-11-09 不列颠哥伦比亚大学 一次性微流控盒
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
US10730044B2 (en) 2015-10-01 2020-08-04 The Regents Of The University Of Michigan Assay plate and uses thereof
US11123297B2 (en) 2015-10-13 2021-09-21 President And Fellows Of Harvard College Systems and methods for making and using gel microspheres
US11092607B2 (en) 2015-10-28 2021-08-17 The Board Institute, Inc. Multiplex analysis of single cell constituents
WO2017075297A1 (en) 2015-10-28 2017-05-04 The Broad Institute Inc. High-throughput dynamic reagent delivery system
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
KR102723865B1 (ko) 2015-12-04 2024-10-29 10엑스 제노믹스, 인크. 핵산 분석을 위한 방법 및 조성물
WO2017103967A1 (ja) * 2015-12-14 2017-06-22 株式会社日立製作所 液滴生成装置、液滴生成方法、及び液体処理システム
WO2017117647A1 (en) 2016-01-06 2017-07-13 The University Of British Columbia Bifurcating mixers and methods of their use and manufacture
EP3400298B1 (en) 2016-01-08 2024-03-06 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
US12071663B2 (en) 2016-01-15 2024-08-27 Massachusetts Institute Of Technology Semi-permeable arrays for analyzing biological systems and methods of using same
JP6735348B2 (ja) 2016-02-11 2020-08-05 10エックス ジェノミクス, インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
WO2017151098A1 (en) * 2016-02-29 2017-09-08 Hewlett-Packard Development Company, L.P. Liquid directing sample container
WO2017165620A1 (en) 2016-03-24 2017-09-28 Bio-Rad Laboratories, Inc. Use of gel beads to control droplet dispersion
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US11142791B2 (en) 2016-08-10 2021-10-12 The Regents Of The University Of California Combined multiple-displacement amplification and PCR in an emulsion microdroplet
CN110312825A (zh) 2016-10-24 2019-10-08 吉内恩福赛克公司 隐藏核酸内存在的信息
AU2017382905A1 (en) 2016-12-21 2019-07-04 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018115978A2 (en) * 2016-12-23 2018-06-28 University Of Limerick Water-in-oil-in water emulsions for analysis of biological and chemical samples
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP4310183B1 (en) 2017-01-30 2025-07-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
WO2018145041A1 (en) 2017-02-06 2018-08-09 10X Genomics, Inc. Systems and methods for nucleic acid preparation
WO2018148693A1 (en) 2017-02-13 2018-08-16 Bio-Rad Laboratories, Inc. System, method, and device for forming an array of emulsions
JP6710850B1 (ja) * 2017-04-20 2020-06-17 バイオフルイディカ、インコーポレイテッド 液体サンプルからバイオマーカーを単離するための流体密封性フローシステム
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
EP3625353B1 (en) 2017-05-18 2022-11-30 10X Genomics, Inc. Methods and systems for sorting droplets and beads
WO2018213774A1 (en) 2017-05-19 2018-11-22 10X Genomics, Inc. Systems and methods for analyzing datasets
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US10549279B2 (en) 2017-08-22 2020-02-04 10X Genomics, Inc. Devices having a plurality of droplet formation regions
TWI738328B (zh) * 2017-09-28 2021-09-01 美商伊路米納有限公司 流體施配器總成與用於將流體施配至流體匣中的方法
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10501739B2 (en) 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
CN111479631B (zh) 2017-10-27 2022-02-22 10X基因组学有限公司 用于样品制备和分析的方法和系统
WO2019086018A1 (zh) * 2017-11-06 2019-05-09 北京新羿生物科技有限公司 微液滴生成装置
CN109772480B (zh) * 2017-11-15 2020-11-10 中国科学院青岛生物能源与过程研究所 单个微粒包裹液滴在微流控芯片中形成并分别导出的方法
EP3625361A1 (en) 2017-11-15 2020-03-25 10X Genomics, Inc. Functionalized gel beads
WO2019099908A1 (en) 2017-11-17 2019-05-23 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
US11618023B2 (en) * 2017-12-06 2023-04-04 Samplix Aps Microfluidic device and a method for provision of emulsion droplets
US11779923B2 (en) 2017-12-06 2023-10-10 Samplix Aps Microfluidic device and a method for provision of double emulsion droplets
CN118818037A (zh) 2017-12-12 2024-10-22 10X基因组学有限公司 用于单细胞处理的系统和方法
WO2019126789A1 (en) 2017-12-22 2019-06-27 10X Genomics, Inc. Systems and methods for processing nucleic acid molecules from one or more cells
EP3752832A1 (en) 2018-02-12 2020-12-23 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
EP3775271B1 (en) 2018-04-06 2025-03-12 10X Genomics, Inc. Systems and methods for quality control in single cell processing
CN112399882B (zh) 2018-04-16 2023-08-01 派特恩生物技术有限公司 用于形成二维微滴阵列的方法和设备
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US12163179B2 (en) 2018-08-03 2024-12-10 10X Gemomics, Inc. Methods and systems to minimize barcode exchange
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US11130120B2 (en) * 2018-10-01 2021-09-28 Lifeng XIAO Micro-pipette tip for forming micro-droplets
US10486155B1 (en) 2018-10-22 2019-11-26 Klaris Corporation Vacuum-loaded, droplet-generating microfluidic chips and related methods
CA3119623A1 (en) * 2018-11-13 2020-05-22 National Research Council Of Canada World-to-chip automated interface for centrifugal microfluidic platforms
CN113661005A (zh) * 2018-11-27 2021-11-16 斯蒂拉科技公司 微流控芯片中优化样品加载的孔
CN114040816B (zh) * 2018-11-27 2023-05-12 斯蒂拉科技公司 具有优化的相流的微流控芯片架构
CN109395788B (zh) * 2018-11-28 2024-04-09 西安交通大学 一种管内液滴制备芯片装置
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
TWI725686B (zh) 2018-12-26 2021-04-21 財團法人工業技術研究院 用於產生液珠的管狀結構及液珠產生方法
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US12109565B2 (en) 2019-01-31 2024-10-08 Samplix Aps Microfluidic device and a method for provision of emulsion droplets
US12138629B2 (en) * 2019-01-31 2024-11-12 Samplix Aps Microfluidic device and a method for provision of double emulsion droplets
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
SG11202111242PA (en) 2019-03-11 2021-11-29 10X Genomics Inc Systems and methods for processing optically tagged beads
EP3947722A1 (en) 2019-03-27 2022-02-09 10X Genomics, Inc. Systems and methods for processing rna from cells
US12330156B2 (en) * 2019-04-02 2025-06-17 Shimadzu Corporation Flow path device, and testing device and testing method using same
DE102019003135A1 (de) * 2019-05-03 2020-11-05 Innome Gmbh Mikrotiterplatte
AU2020280104A1 (en) 2019-05-22 2022-01-20 Mission Bio, Inc. Method and apparatus for simultaneous targeted sequencing of DNA, RNA and protein
WO2021003255A1 (en) 2019-07-01 2021-01-07 Mission Bio Method and apparatus to normalize quantitative readouts in single-cell experiments
WO2021018111A1 (en) * 2019-07-29 2021-02-04 Bgi Shenzhen Co., Ltd. Reagent cartridges for in-vitro devices
DK182109B1 (en) * 2019-08-02 2025-08-18 Samplix Aps System and method for amplifying nucleic acids from single cells
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
AU2020361681A1 (en) 2019-10-10 2022-05-05 1859, Inc. Methods and systems for microfluidic screening
KR102227970B1 (ko) * 2019-11-26 2021-03-15 주식회사 바이오티엔에스 미세 액적 생성 장치
WO2021119201A1 (en) 2019-12-10 2021-06-17 Enumerix, Inc. Methods and systems for three-dimensional lightsheet imaging
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
EP4104179B1 (en) 2020-02-13 2026-01-28 10X Genomics, Inc. Systems and methods for joint interactive visualization of gene expression and dna chromatin accessibility
US12023637B2 (en) 2020-03-23 2024-07-02 Mark A. Gray Capillary tube droplet generation systems and methods
US10953404B1 (en) 2020-04-24 2021-03-23 Pattern Bioscience, Inc. Apparatuses for contactless loading and imaging of microfluidic chips and related methods
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
EP4164795A4 (en) 2020-06-12 2024-01-24 BioFluidica, Inc. DUAL-DEPTH THERMOPLASTIC MICROFLUIDIC DEVICE AND ASSOCIATED SYSTEMS AND METHODS
US11260639B2 (en) 2020-08-03 2022-03-01 Google Llc Apparatus for reducing fabric dimpling in electronic devices and associated methods
JP7440905B2 (ja) 2020-08-25 2024-02-29 株式会社オンチップ・バイオテクノロジーズ 粒子の純化方法、単一粒子分注方法、及び細胞クラスター解析方法、並びそれに用いる装置
US12655450B1 (en) 2020-10-27 2026-06-16 10X Genomics, Inc. Methods, systems, and kits for detection of nucleic acid molecules
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
WO2022187684A1 (en) 2021-03-05 2022-09-09 Enumerix, Inc. Systems and methods for generating droplets and performing digital analyses
US12540948B2 (en) 2021-03-05 2026-02-03 Countable Labs, Inc. Systems and methods for generating droplets and performing digital analyses
EP4088814A3 (en) * 2021-04-20 2023-01-25 National Research Council of Canada Microfluidic chip, kit, and system for displacing independent reaction volumes of an emulsion
US12252745B2 (en) 2021-09-02 2025-03-18 Enumerix, Inc. Detection and digital quantitation of multiple targets
EP4453246A4 (en) 2021-12-20 2025-12-24 Countable Labs Inc DIGITAL DETECTION AND QUANTIFICATION OF MULTIPLE TARGETS
US20240198336A1 (en) 2022-12-19 2024-06-20 Anchorline Biolabs, Inc. Capillary partitioning microfluidics
EP4578547A1 (en) 2023-12-27 2025-07-02 Tecan Trading AG Microfluidic device for a laboratory automation device
GB202415712D0 (en) * 2024-10-25 2024-12-11 Univ Dublin A microfluidic system and process for high thrtoughput synthesis of nanopatricles

Family Cites Families (312)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3575220A (en) 1968-08-12 1971-04-20 Scientific Industries Apparatus for dispensing liquid sample
BR7500831A (pt) 1974-02-11 1975-12-02 Fmc Corp Aparelho de separacao de bolhas de gas corpo de transmissao de gas para uso no aparelho processo para fabricacao do dito corpo e para difusao de bolhas finas de gas em um corpo de liquido
US4051025A (en) 1976-09-29 1977-09-27 The United States Of America As Represented By The Department Of Health, Education And Welfare Preparative countercurrent chromatography with a slowly rotating helical tube array
US4201691A (en) 1978-01-16 1980-05-06 Exxon Research & Engineering Co. Liquid membrane generator
US4348111A (en) 1978-12-07 1982-09-07 The English Electric Company Limited Optical particle analyzers
US4283262A (en) 1980-07-01 1981-08-11 Instrumentation Laboratory Inc. Analysis system
GB2097692B (en) 1981-01-10 1985-05-22 Shaw Stewart P D Combining chemical reagents
US4399219A (en) 1981-01-29 1983-08-16 Massachusetts Institute Of Technology Process for isolating microbiologically active material
US4636075A (en) 1984-08-22 1987-01-13 Particle Measuring Systems, Inc. Particle measurement utilizing orthogonally polarized components of a laser beam
US4948961A (en) 1985-08-05 1990-08-14 Biotrack, Inc. Capillary flow device
US5225332A (en) 1988-04-22 1993-07-06 Massachusetts Institute Of Technology Process for manipulation of non-aqueous surrounded microdroplets
US5055390A (en) 1988-04-22 1991-10-08 Massachusetts Institute Of Technology Process for chemical manipulation of non-aqueous surrounded microdroplets
JPH082416B2 (ja) 1988-09-29 1996-01-17 宮崎県 エマルションの製造方法
ES2060184T3 (es) 1989-06-22 1994-11-16 Atta Moleculas anfifilicas conteniendo fluor y fosforo con propiedades tensoactivas.
GB8917963D0 (en) 1989-08-05 1989-09-20 Scras Apparatus for repeated automatic execution of a thermal cycle for treatment of biological samples
US6176962B1 (en) 1990-02-28 2001-01-23 Aclara Biosciences, Inc. Methods for fabricating enclosed microchannel structures
GB9016163D0 (en) 1990-07-24 1990-09-05 Cemu Bioteknik Chemical method
KR100236506B1 (ko) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
US5270183A (en) 1991-02-08 1993-12-14 Beckman Research Institute Of The City Of Hope Device and method for the automated cycling of solutions between two or more temperatures
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
EP0519338B1 (en) 1991-06-20 1996-08-28 F. Hoffmann-La Roche Ag Improved methods for nucleic acid amplification
US5422277A (en) 1992-03-27 1995-06-06 Ortho Diagnostic Systems Inc. Cell fixative composition and method of staining cells without destroying the cell surface
US5587128A (en) 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5637469A (en) 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
US5639423A (en) 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
DE69429038T2 (de) 1993-07-28 2002-03-21 Pe Corporation (Ny), Norwalk Vorrichtung und Verfahren zur Nukleinsäurevervielfältigung
US5538667A (en) 1993-10-28 1996-07-23 Whitehill Oral Technologies, Inc. Ultramulsions
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
JP2909216B2 (ja) 1994-04-29 1999-06-23 パーキン‐エルマー コーポレイション 核酸増幅生成物のリアルタイム検出装置
US5972716A (en) 1994-04-29 1999-10-26 The Perkin-Elmer Corporation Fluorescence monitoring device with textured optical tube and method for reducing background fluorescence
DE69527585T2 (de) 1994-06-08 2003-04-03 Affymetrix, Inc. Verfahren und Vorrichtung zum Verpacken von Chips
JPH0835971A (ja) 1994-07-25 1996-02-06 Hitachi Ltd ピペット
US5555191A (en) 1994-10-12 1996-09-10 Trustees Of Columbia University In The City Of New York Automated statistical tracker
FI100859B (sv) 1994-10-14 1998-03-13 Bjarne Holmbom Förfarande och anordning för att utföra on-line flödesextraktion av ex traherbara komponenter i vätskor
US5585069A (en) 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
FR2733766B1 (fr) 1995-05-03 1997-06-06 Rhone Poulenc Rorer Sa Methode de diagnostic de la schizophrenie
US20020022261A1 (en) 1995-06-29 2002-02-21 Anderson Rolfe C. Miniaturized genetic analysis systems and methods
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US6130098A (en) 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
US6057149A (en) 1995-09-15 2000-05-02 The University Of Michigan Microscale devices and reactions in microscale devices
US20020068357A1 (en) 1995-09-28 2002-06-06 Mathies Richard A. Miniaturized integrated nucleic acid processing and analysis device and method
US6562605B1 (en) 1995-11-13 2003-05-13 Genencor International, Inc. Extraction of water soluble biomaterials from fluids using a carbon dioxide/surfactant mixture
US5736314A (en) 1995-11-16 1998-04-07 Microfab Technologies, Inc. Inline thermo-cycler
EP0889751B1 (en) 1996-04-03 1999-09-08 The Perkin-Elmer Corporation Device and method for multiple analyte detection
CN1173776C (zh) 1996-06-28 2004-11-03 卡钳技术有限公司 在微规模流体性设备里的高通过量的筛选分析系统
JP3361530B2 (ja) * 1996-06-28 2003-01-07 カリパー・テクノロジーズ・コープ. 電子ピペッタおよび電気泳動バイアスのための補償手段
US7026468B2 (en) 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US6558916B2 (en) 1996-08-02 2003-05-06 Axiom Biotechnologies, Inc. Cell flow apparatus and method for real-time measurements of patient cellular responses
GB9621357D0 (en) 1996-10-12 1996-12-04 Central Research Lab Ltd Heating apparatus
US7268179B2 (en) 1997-02-03 2007-09-11 Cytonix Corporation Hydrophobic coating compositions, articles coated with said compositions, and processes for manufacturing same
EP2327797B1 (en) 1997-04-01 2015-11-25 Illumina Cambridge Limited Method of nucleic acid sequencing
JP2001517948A (ja) 1997-04-01 2001-10-09 グラクソ、グループ、リミテッド 核酸配列決定法
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
JP2002503336A (ja) 1997-05-16 2002-01-29 アルバータ リサーチ カウンシル 微量流通システムおよびその使用方法
JP3120453B2 (ja) 1997-06-19 2000-12-25 トヨタ自動車株式会社 微小液滴の保持方法、反応方法
US5912945A (en) 1997-06-23 1999-06-15 Regents Of The University Of California X-ray compass for determining device orientation
ATE358177T1 (de) 1997-07-07 2007-04-15 Medical Res Council Ein in vitro sortierverfahren
US5980936A (en) 1997-08-07 1999-11-09 Alliance Pharmaceutical Corp. Multiple emulsions comprising a hydrophobic continuous phase
ES2243007T3 (es) 1997-09-16 2005-11-16 Egea Biosciences Llc Sintesis quimica completa y sintesis de genes de genomas.
US6540895B1 (en) 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
US6833242B2 (en) 1997-09-23 2004-12-21 California Institute Of Technology Methods for detecting and sorting polynucleotides based on size
US6074725A (en) 1997-12-10 2000-06-13 Caliper Technologies Corp. Fabrication of microfluidic circuits by printing techniques
US7497994B2 (en) 1998-02-24 2009-03-03 Khushroo Gandhi Microfluidic devices and systems incorporating cover layers
GB9804483D0 (en) 1998-03-02 1998-04-29 Central Research Lab Ltd Apparatus for and method of controlling the rate of flow of fluid along a pathway
ES2277430T3 (es) 1998-03-04 2007-07-01 Visx Incorporated Sistema de tratamiento con laser de la presbicia.
US6175669B1 (en) 1998-03-30 2001-01-16 The Regents Of The Universtiy Of California Optical coherence domain reflectometry guidewire
JP3081880B2 (ja) 1998-03-30 2000-08-28 農林水産省食品総合研究所長 マイクロスフィアの連続製造装置
US6384915B1 (en) 1998-03-30 2002-05-07 The Regents Of The University Of California Catheter guided by optical coherence domain reflectometry
US6123798A (en) 1998-05-06 2000-09-26 Caliper Technologies Corp. Methods of fabricating polymeric structures incorporating microscale fluidic elements
CN2364458Y (zh) 1998-09-10 2000-02-16 北京万拓仪器有限公司 用于原子荧光光度计的断续流动装置
JP3012608B1 (ja) 1998-09-17 2000-02-28 農林水産省食品総合研究所長 マイクロチャネル装置及び同装置を用いたエマルションの製造方法
US6146103A (en) 1998-10-09 2000-11-14 The Regents Of The University Of California Micromachined magnetohydrodynamic actuators and sensors
US6601613B2 (en) * 1998-10-13 2003-08-05 Biomicro Systems, Inc. Fluid circuit components based upon passive fluid dynamics
US6176609B1 (en) 1998-10-13 2001-01-23 V & P Scientific, Inc. Magnetic tumble stirring method, devices and machines for mixing in vessels
US6637463B1 (en) 1998-10-13 2003-10-28 Biomicro Systems, Inc. Multi-channel microfluidic system design with balanced fluid flow distribution
US6086740A (en) 1998-10-29 2000-07-11 Caliper Technologies Corp. Multiplexed microfluidic devices and systems
US6261431B1 (en) 1998-12-28 2001-07-17 Affymetrix, Inc. Process for microfabrication of an integrated PCR-CE device and products produced by the same
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US6522407B2 (en) 1999-01-22 2003-02-18 The Regents Of The University Of California Optical detection dental disease using polarized light
US6326083B1 (en) 1999-03-08 2001-12-04 Calipher Technologies Corp. Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety
US6303343B1 (en) 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
US6964846B1 (en) 1999-04-09 2005-11-15 Exact Sciences Corporation Methods for detecting nucleic acids indicative of cancer
US6357907B1 (en) 1999-06-15 2002-03-19 V & P Scientific, Inc. Magnetic levitation stirring devices and machines for mixing in vessels
DE60038883D1 (de) * 1999-06-22 2008-06-26 Tecan Trading Ag Vorrichtungen zur durchfuehrung von miniaturisierten in vitro amplifizierungsassays
US6977145B2 (en) 1999-07-28 2005-12-20 Serono Genetics Institute S.A. Method for carrying out a biochemical protocol in continuous flow in a microreactor
WO2001007159A2 (en) 1999-07-28 2001-02-01 Genset Integration of biochemical protocols in a continuous flow microfluidic device
US6440706B1 (en) 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
DE19964337B4 (de) 1999-10-01 2004-09-16 Agilent Technologies, Inc. (n.d.Ges.d.Staates Delaware), Palo Alto Mikrofluidischer Mikrochip mit abbiegbarem Ansaugrohr
ATE458831T1 (de) 1999-12-07 2010-03-15 Exact Sciences Corp Verfahren zum nachweis von lungenneoplasmen in fäkalen proben
ATE340025T1 (de) 2000-01-06 2006-10-15 Caliper Life Sciences Inc Vorrichtungen und verfahren für hochdurchsatz- probenentnahme und analyse
JP2003522963A (ja) 2000-02-18 2003-07-29 アクララ バイオサイエンシーズ, インコーポレイテッド 複数部位反応デバイスおよび方法
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
MXPA02008290A (es) 2000-02-23 2004-09-10 Caliper Technologies Inc Sistema de control de presion de multi-reserva-.
JP3282619B2 (ja) 2000-03-22 2002-05-20 住友電装株式会社 ワイヤーハーネスの屈曲試験方法及び電線の屈曲試験装置
US20010048900A1 (en) 2000-05-24 2001-12-06 Bardell Ronald L. Jet vortex mixer
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
US6466713B2 (en) 2000-08-18 2002-10-15 The Regents Of The University Of California Optical fiber head for providing lateral viewing
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
ATE448875T1 (de) 2000-09-14 2009-12-15 Caliper Life Sciences Inc Mikrofluidische vorrichtungen und methoden um temperatur-vermittelte reaktionen durchzuführen
US7294503B2 (en) 2000-09-15 2007-11-13 California Institute Of Technology Microfabricated crossflow devices and methods
US20020142483A1 (en) 2000-10-30 2002-10-03 Sequenom, Inc. Method and apparatus for delivery of submicroliter volumes onto a substrate
AU2002239566A1 (en) 2000-11-09 2002-05-27 Therics, Inc. Method and apparatus for obtaining information about a dispensed fluid during printing
KR100435921B1 (ko) 2000-12-29 2004-06-12 주식회사 태평양 동수역학적 이중 안정화에 의한 안정한 수-유-수 다중에멀젼 시스템 및 이의 제조방법
KR100860291B1 (ko) 2001-01-19 2008-09-25 에게아 바이오사이언시스, 인크. 표적 폴리펩티드를 암호화하는 폴리뉴클레오티드의 컴퓨터지시된 어셈블리
US7567596B2 (en) 2001-01-30 2009-07-28 Board Of Trustees Of Michigan State University Control system and apparatus for use with ultra-fast laser
AU2002243764A1 (en) 2001-01-31 2002-08-19 Sau Lan Tang Staats Microfluidic devices
EP1721658B2 (en) 2001-02-23 2020-08-05 Japan Science and Technology Agency Process and apparatus for producing microcapsules
US6949176B2 (en) 2001-02-28 2005-09-27 Lightwave Microsystems Corporation Microfluidic control using dielectric pumping
WO2002072264A1 (en) * 2001-03-09 2002-09-19 Biomicro Systems, Inc. Method and system for microfluidic interfacing to arrays
US7192557B2 (en) 2001-03-28 2007-03-20 Handylab, Inc. Methods and systems for releasing intracellular material from cells within microfluidic samples of fluids
US7270786B2 (en) 2001-03-28 2007-09-18 Handylab, Inc. Methods and systems for processing microfluidic samples of particle containing fluids
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US6575188B2 (en) 2001-07-26 2003-06-10 Handylab, Inc. Methods and systems for fluid control in microfluidic devices
AU2002307152A1 (en) 2001-04-06 2002-10-21 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
US6793723B2 (en) 2001-05-10 2004-09-21 Pitney Bowes Inc. Homogeneous photosensitive optically variable ink compositions for ink jet printing
US20030049659A1 (en) 2001-05-29 2003-03-13 Lapidus Stanley N. Devices and methods for isolating samples into subsamples for analysis
US6905885B2 (en) 2001-06-12 2005-06-14 The Regents Of The University Of California Portable pathogen detection system
US20030013680A1 (en) 2001-06-12 2003-01-16 Keryx Methods using glycosaminoglycans for the treatment of nephropathy
US6808683B2 (en) * 2001-09-25 2004-10-26 Cytonome, Inc. Droplet dispensing system
JP4537053B2 (ja) 2001-06-25 2010-09-01 ジョージア テック リサーチ コーポレーション 二重共鳴エネルギー転移核酸プローブ
US6797257B2 (en) 2001-06-26 2004-09-28 The Board Of Trustees Of The University Of Illinois Paramagnetic polymerized protein microspheres and methods of preparation thereof
US6573490B2 (en) 2001-06-28 2003-06-03 Valeo Electrical Systems, Inc. Interleaved mosaic imaging rain sensor
US20030032172A1 (en) 2001-07-06 2003-02-13 The Regents Of The University Of California Automated nucleic acid assay system
US7297778B2 (en) 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
US20030027150A1 (en) 2001-08-03 2003-02-06 Katz David A. Method of haplotyping and kit therefor
US7060227B2 (en) 2001-08-06 2006-06-13 Sau Lan Tang Staats Microfluidic devices with raised walls
AUPR707101A0 (en) 2001-08-16 2001-09-06 Corbett Research Pty Ltd Continuous flow thermal device
US7094379B2 (en) 2001-10-24 2006-08-22 Commissariat A L'energie Atomique Device for parallel and synchronous injection for sequential injection of different reagents
KR100442836B1 (ko) 2001-11-10 2004-08-02 삼성전자주식회사 생화학 유체를 온도가 다른 폐쇄된 챔버 구간을 따라 회전이동시키는 폐쇄 유체 회로 시스템
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
EP1463796B1 (en) 2001-11-30 2013-01-09 Fluidigm Corporation Microfluidic device and methods of using same
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
WO2003057010A2 (en) 2002-01-04 2003-07-17 Board Of Regents, The University Of Texas System Droplet-based microfluidic oligonucleotide synthesis engine
US20030180765A1 (en) 2002-02-01 2003-09-25 The Johns Hopkins University Digital amplification for detection of mismatch repair deficient tumor cells
US20040109793A1 (en) 2002-02-07 2004-06-10 Mcneely Michael R Three-dimensional microfluidics incorporating passive fluid control structures
US7470518B2 (en) 2002-02-12 2008-12-30 Cellectricon Ab Systems and method for rapidly changing the solution environment around sensors
DE60303504T2 (de) 2002-02-22 2006-10-12 Biodot, Inc., Irvine Verfahren und vorrichtung zur kontaktlosen tröpfchenabgabe unterhalb einer fluidischen oberfläche
CN1656234B (zh) 2002-03-20 2012-02-01 创新生物公司 包囊化核酸扩增反应混合物的微胶囊及其作为反应隔间进行平行反应的用途
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
US7993266B2 (en) 2002-04-26 2011-08-09 Lawrence Livermore National Security, Llc Early detection of contagious diseases
EP1508044B1 (en) 2002-05-09 2010-09-01 The University of Chicago Device and method for pressure-driven plug transport and reaction
FR2841063B1 (fr) 2002-06-18 2004-09-17 Commissariat Energie Atomique Dispositif de deplacement de petits volumes de liquide le long d'un micro-catenaire par des forces electrostatiques
JP2005530183A (ja) 2002-06-20 2005-10-06 バイオヴェリス コーポレイション エレクトロケミルミネッセンス・フローセル及びフローセル部品
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
US6794671B2 (en) 2002-07-17 2004-09-21 Particle Sizing Systems, Inc. Sensors and methods for high-sensitivity optical particle counting and sizing
US7188731B2 (en) 2002-08-26 2007-03-13 The Regents Of The University Of California Variable flexure-based fluid filter
US20040038385A1 (en) 2002-08-26 2004-02-26 Langlois Richard G. System for autonomous monitoring of bioagents
JP5695287B2 (ja) 2002-10-02 2015-04-01 カリフォルニア インスティテュート オブ テクノロジー 微小流体の核酸解析
US20050266582A1 (en) 2002-12-16 2005-12-01 Modlin Douglas N Microfluidic system with integrated permeable membrane
US20060094108A1 (en) 2002-12-20 2006-05-04 Karl Yoder Thermal cycler for microfluidic array assays
JP4395133B2 (ja) 2002-12-20 2010-01-06 カリパー・ライフ・サイエンシズ・インク. Dnaの単一分子増幅および検出
EP1608952B1 (en) * 2002-12-20 2016-08-10 Life Technologies Corporation Assay apparatus and method using microfluidic arrays
US20050042639A1 (en) 2002-12-20 2005-02-24 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA length
DE602004026033D1 (de) 2003-01-29 2010-04-29 454 Corp Zweiendige sequenzierung
WO2004071638A2 (en) 2003-02-11 2004-08-26 Regents Of The University Of California, The Microfluidic devices and method for controlled viscous shearing and formation of amphiphilic vesicles
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
DK2308419T3 (da) 2003-03-28 2016-06-06 Inguran Llc Fremgangsmåde til bedømmelse af farvningstilstandene for dyresperm, som skal sorteres.
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
DE10315640A1 (de) 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
WO2004094020A2 (en) 2003-04-17 2004-11-04 Fluidigm Corporation Crystal growth devices and systems, and methods for using same
US7629123B2 (en) 2003-07-03 2009-12-08 University Of Medicine And Dentistry Of New Jersey Compositions and methods for diagnosing autism
DE602004028837D1 (de) 2003-07-03 2010-10-07 Univ New Jersey Med Gene als diagnostische werkzeuge für autismus
US8048627B2 (en) 2003-07-05 2011-11-01 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
AU2004262406B2 (en) 2003-08-01 2008-07-03 Ge Healthcare Bio-Sciences Ab Optical resonance analysis unit
CN1842368B (zh) 2003-08-27 2014-05-28 哈佛大学 流体物种的电子控制
AU2004270220B2 (en) 2003-09-05 2009-03-05 The Chinese University Of Hong Kong Method for non-invasive prenatal diagnosis
US7417726B2 (en) 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
US20050277125A1 (en) 2003-10-27 2005-12-15 Massachusetts Institute Of Technology High-density reaction chambers and methods of use
US7141537B2 (en) 2003-10-30 2006-11-28 3M Innovative Properties Company Mixture of fluorinated polyethers and use thereof as surfactant
ES2355242T3 (es) 2003-12-05 2011-03-24 Beatrice And Samuel A. Seaver Foundation Procedimiento de evaluación del riesgo genético relativo al autismo.
WO2005073410A2 (en) 2004-01-28 2005-08-11 454 Corporation Nucleic acid amplification with continuous flow emulsion
US8293471B2 (en) 2004-01-28 2012-10-23 Marshall University Research Corporation Apparatus and method for a continuous rapid thermal cycle system
WO2005075683A1 (en) 2004-02-03 2005-08-18 Postech Foundation High throughput device for performing continuous-flow reactions
US8101431B2 (en) * 2004-02-27 2012-01-24 Board Of Regents, The University Of Texas System Integration of fluids and reagents into self-contained cartridges containing sensor elements and reagent delivery systems
KR100552706B1 (ko) 2004-03-12 2006-02-20 삼성전자주식회사 핵산 증폭 방법 및 장치
CN100431679C (zh) 2004-03-23 2008-11-12 独立行政法人科学技术振兴机构 微小液滴的生成方法及装置
US7432106B2 (en) * 2004-03-24 2008-10-07 Applied Biosystems Inc. Liquid processing device including gas trap, and system and method
EP2439284B1 (en) 2004-03-31 2019-05-08 The General Hospital Corporation Method to determine responsiveness of cancer to epidermal growth factor receptor targeting treatments
US20050221279A1 (en) 2004-04-05 2005-10-06 The Regents Of The University Of California Method for creating chemical sensors using contact-based microdispensing technology
HU227246B1 (en) 2004-04-30 2010-12-28 Szegedi Biolog Koezpont Use of genes as molecular markers in diagnosis of schizophrenia and diagnostic kit
WO2005110395A1 (en) 2004-05-19 2005-11-24 University Of South Carolina System and device for magnetic drug targeting with magnetic drug carrier particles
WO2006002167A2 (en) 2004-06-17 2006-01-05 University Of Florida Research Foudation, Inc. Multi-acceptor molecular probes and applications thereof
DE602005013153D1 (de) 2004-06-29 2009-04-23 Roche Diagnostics Gmbh Assoziation von SNPs in PPAR gamma mit Osteoporose
WO2006023719A2 (en) 2004-08-20 2006-03-02 Enh Research Institute Identification of snp’s associated with schizophrenia, schizoaffective disorder and bipolar disorder
JP5885901B2 (ja) 2004-09-09 2016-03-16 アンスティテュート キュリー マイクロチャネルまたは他のマイクロ容器中でパケットを操作するためのデバイス
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
WO2006047787A2 (en) 2004-10-27 2006-05-04 Exact Sciences Corporation Method for monitoring disease progression or recurrence
US8951746B2 (en) 2004-11-05 2015-02-10 Southwest Research Institute Method and devices for screening cervical cancer
US20090098044A1 (en) 2004-11-15 2009-04-16 Australian Nuclear Science And Technology Organisation Solid particles from controlled destabilisation of microemulsions
JP4601423B2 (ja) * 2004-12-28 2010-12-22 独立行政法人科学技術振興機構 細胞計測および分離チップ
US20060166223A1 (en) * 2005-01-26 2006-07-27 Reed Michael W DNA purification and analysis on nanoengineered surfaces
US7423751B2 (en) 2005-02-08 2008-09-09 Northrop Grumman Corporation Systems and methods for use in detecting harmful aerosol particles
WO2006086777A2 (en) 2005-02-11 2006-08-17 Memorial Sloan Kettering Cancer Center Methods and compositions for detecting a drug resistant egfr mutant
US9039273B2 (en) 2005-03-04 2015-05-26 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
US20070054119A1 (en) * 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
RU2400538C2 (ru) 2005-03-05 2010-09-27 Сиджен, Инк. Олигонуклеотид с двойственной специфичностью и способы, в которых он используется
WO2006099579A2 (en) 2005-03-16 2006-09-21 Applera Corporation Compositions and methods for clonal amplification and analysis of polynucleotides
US20070048756A1 (en) 2005-04-18 2007-03-01 Affymetrix, Inc. Methods for whole genome association studies
WO2006128098A2 (en) 2005-05-27 2006-11-30 Applera Corporation Cytometric system including nucleic acid sequence amplification, and method
DE102005037401B4 (de) 2005-08-08 2007-09-27 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Bildung einer Emulsion in einem fluidischen Mikrosystem
US20070068573A1 (en) * 2005-08-22 2007-03-29 Applera Corporation Device and method for microfluidic control of a first fluid in contact with a second fluid, wherein the first and second fluids are immiscible
AU2006294565A1 (en) 2005-09-30 2007-04-05 Perlegen Sciences, Inc. Methods and compositions for screening and treatment of disorders of blood glucose regulation
JP4751718B2 (ja) 2005-12-28 2011-08-17 株式会社島津製作所 遺伝子解析装置
WO2007081385A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US7976795B2 (en) 2006-01-19 2011-07-12 Rheonix, Inc. Microfluidic systems
WO2007089541A2 (en) 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
WO2007092473A2 (en) 2006-02-02 2007-08-16 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive fetal genetic screening by digital analysis
EP2298438A1 (en) 2006-02-07 2011-03-23 Stokes Bio Limited A microfluidic droplet queuing network
US20100304446A1 (en) 2006-02-07 2010-12-02 Stokes Bio Limited Devices, systems, and methods for amplifying nucleic acids
EP1991357B1 (en) 2006-02-07 2016-09-14 Stokes Bio Limited A microfluidic analysis system
WO2007091229A1 (en) * 2006-02-07 2007-08-16 Stokes Bio Limited A microfluidic droplet queuing network
US7598091B2 (en) * 2006-04-04 2009-10-06 Micropoint Bioscience, Inc. Micromachined diagnostic device with controlled flow of fluid and reaction
WO2007120745A2 (en) 2006-04-11 2007-10-25 Optiscan Biomedical Corporation Noise reduction for analyte detection system
US8492168B2 (en) 2006-04-18 2013-07-23 Advanced Liquid Logic Inc. Droplet-based affinity assays
US7815871B2 (en) 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet microactuator system
US7816121B2 (en) 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet actuation system and method
AU2007237909A1 (en) 2006-04-19 2007-10-25 Applied Biosystems, Llc. Reagents, methods, and libraries for gel-free bead-based sequencing
US7470403B2 (en) * 2006-04-26 2008-12-30 Wisconsin Alumni Research Foundation Microfluidic platform and method of generating a gradient therein
WO2007133710A2 (en) 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
WO2007150030A2 (en) 2006-06-23 2007-12-27 Massachusetts Institute Of Technology Microfluidic synthesis of organic nanoparticles
US7608399B2 (en) 2006-06-26 2009-10-27 Blood Cell Storage, Inc. Device and method for extraction and analysis of nucleic acids from biological samples
EP1881079A1 (en) 2006-07-20 2008-01-23 Pangaea Biotech, S.A. Method for the detection of EGFR mutations in blood samples
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
WO2008024114A1 (en) 2006-08-24 2008-02-28 Genizon Biosciences Inc. Genemap of the human genes associated with schizophrenia
WO2008030433A2 (en) 2006-09-06 2008-03-13 Canon U.S. Life Sciences, Inc. Chip and cartridge design configuration for performing micro-fluidic assays
US20080166720A1 (en) 2006-10-06 2008-07-10 The Regents Of The University Of California Method and apparatus for rapid nucleic acid analysis
EP1912067A1 (en) 2006-10-12 2008-04-16 Eppendorf Array Technologies S.A. Method for quantification of a target compound obtained from a biological sample upon chips
US8841116B2 (en) 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
WO2008061165A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic cartridge and method of making same
WO2008070074A2 (en) 2006-12-04 2008-06-12 Pgxhealth Llc Genetic markers of schizophrenia
WO2008070862A2 (en) 2006-12-07 2008-06-12 Biocept, Inc. Non-invasive prenatal genetic screen
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8338166B2 (en) 2007-01-04 2012-12-25 Lawrence Livermore National Security, Llc Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
WO2008089244A2 (en) * 2007-01-17 2008-07-24 University Of Rochester Frequency-addressable apparatus and methods for actuation of liquids
EP2125219B1 (en) * 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
US20090011417A1 (en) 2007-03-07 2009-01-08 George Maltezos Testing Device
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
WO2008112177A2 (en) 2007-03-08 2008-09-18 Genizon Biosciences, Inc. Genemap of the human genes associated with schizophrenia
EP2136786B8 (en) 2007-03-28 2012-11-14 President and Fellows of Harvard College Apparatus for forming droplets
JP2008245612A (ja) 2007-03-30 2008-10-16 Hitachi Ltd 試料調製法および装置
CN101663089A (zh) * 2007-04-04 2010-03-03 微点生物技术有限公司 微机械加工的电润湿微流体阀
JP5666763B2 (ja) 2007-04-11 2015-02-12 味の素株式会社 油中水型乳化組成物
CA2689427C (en) * 2007-06-05 2015-12-29 Eugenia Kumacheva Multiple continuous microfluidic reactors for the scaled up synthesis of gel or polymer particles
WO2009002920A1 (en) 2007-06-22 2008-12-31 Advanced Liquid Logic, Inc. Droplet-based nucleic acid amplification in a temperature gradient
US20090068170A1 (en) 2007-07-13 2009-03-12 President And Fellows Of Harvard College Droplet-based selection
CA2694820C (en) 2007-07-30 2015-06-16 F. Hoffman-La Roche Ag Methods of identification using methylation of cpg
US20090053719A1 (en) 2007-08-03 2009-02-26 The Chinese University Of Hong Kong Analysis of nucleic acids by digital pcr
ES2380844T3 (es) 2007-09-07 2012-05-18 Fluidigm Corporation Determinación de la variación en el número de copias, métodos y sistemas
EP2203567B1 (en) 2007-10-16 2011-05-11 Roche Diagnostics GmbH High resolution, high throughput hla genotyping by clonal sequencing
US7807920B2 (en) 2007-10-30 2010-10-05 Opel, Inc. Concentrated solar photovoltaic module
WO2009085246A1 (en) 2007-12-20 2009-07-09 University Of Massachusetts Cross-linked biopolymers, related compositions and methods of use
JP5224801B2 (ja) 2007-12-21 2013-07-03 キヤノン株式会社 核酸増幅装置
CA2639954C (en) * 2008-02-11 2017-08-15 Aaron R. Wheeler Droplet-based cell culture and cell assays using digital microfluidics
US20090220434A1 (en) 2008-02-29 2009-09-03 Florida State University Research Foundation Nanoparticles that facilitate imaging of biological tissue and methods of forming the same
US8062903B2 (en) * 2008-03-03 2011-11-22 University Of Washington Droplet compartmentalization for chemical separation and on-line sampling
US9487822B2 (en) 2008-03-19 2016-11-08 Fluidigm Corporation Method and apparatus for determining copy number variation using digital PCR
US9011777B2 (en) 2008-03-21 2015-04-21 Lawrence Livermore National Security, Llc Monodisperse microdroplet generation and stopping without coalescence
US8961902B2 (en) 2008-04-23 2015-02-24 Bioscale, Inc. Method and apparatus for analyte processing
EP2672260A1 (en) 2008-05-13 2013-12-11 Advanced Liquid Logic, Inc. Droplet actuator devices, systems and methods
EP2352591A4 (en) * 2008-06-13 2013-01-23 Emerald Biostructures Inc NANOVOLUMIC MICROKAPILLAR CRYSTALLIZATION SYSTEM
WO2010001419A2 (en) 2008-07-04 2010-01-07 Decode Genetics Ehf Copy number variations predictive of risk of schizophrenia
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
US8256931B2 (en) 2008-07-24 2012-09-04 Seward George H Achromatic homogenizer and collimator for LEDs
US20100018584A1 (en) * 2008-07-28 2010-01-28 Technion Research & Development Foundation Ltd. Microfluidic system and method for manufacturing the same
WO2010018465A2 (en) 2008-08-12 2010-02-18 Stokes Bio Limited Methods and devices for digital pcr
US9180453B2 (en) 2008-08-15 2015-11-10 University Of Washington Method and apparatus for the discretization and manipulation of sample volumes
WO2010021936A1 (en) 2008-08-16 2010-02-25 The Board Of Trustees Of The Leland Stanford Junior University Digital pcr calibration for high throughput sequencing
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
GB2502409B8 (en) * 2008-09-23 2014-10-15 Bio Rad Laboratories Droplet-based assay system
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US20130059754A1 (en) 2011-09-01 2013-03-07 Bio-Rad Laboratories, Inc. Digital assays with reduced measurement uncertainty
US20130084572A1 (en) 2011-09-30 2013-04-04 Quantalife, Inc. Calibrations and controls for droplet-based assays
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US20120171683A1 (en) 2010-03-02 2012-07-05 Ness Kevin D Analysis of fragmented genomic dna in droplets
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9089844B2 (en) 2010-11-01 2015-07-28 Bio-Rad Laboratories, Inc. System for forming emulsions
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
US8663920B2 (en) 2011-07-29 2014-03-04 Bio-Rad Laboratories, Inc. Library characterization by digital assay
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US20100156606A1 (en) 2008-12-19 2010-06-24 Gold Steven K RFID Sensor Assemblies and Methods of Use
EP2389455A4 (en) 2009-01-26 2012-12-05 Verinata Health Inc METHODS AND COMPOSITIONS FOR IDENTIFYING A FETAL CELL
US20100217736A1 (en) 2009-02-23 2010-08-26 Oded Sarel Decision support method and apparatus for chaotic or multi-parameter situations
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
US20100261230A1 (en) 2009-04-08 2010-10-14 Applied Biosystems, Llc System comprising dual-sided thermal cycler and emulsion pcr in pouch
US8034629B2 (en) * 2009-06-26 2011-10-11 Massachusetts Institute Of Technology High precision scanning of encoded hydrogel microparticles
CA2767056C (en) 2009-09-02 2018-12-04 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
CA3041540C (en) 2009-09-21 2021-08-24 Bio-Rad Laboratories, Inc. Magnetic lysis method and device
US10520500B2 (en) 2009-10-09 2019-12-31 Abdeslam El Harrak Labelled silica-based nanomaterial with enhanced properties and uses thereof
US9890408B2 (en) 2009-10-15 2018-02-13 Ibis Biosciences, Inc. Multiple displacement amplification
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP2517025B1 (en) 2009-12-23 2019-11-27 Bio-Rad Laboratories, Inc. Methods for reducing the exchange of molecules between droplets
EP4435111A1 (en) 2010-02-12 2024-09-25 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
JP2013524171A (ja) 2010-03-25 2013-06-17 クァンタライフ・インコーポレーテッド 液滴ベースのアッセイのための液滴の発生
JP2013524169A (ja) 2010-03-25 2013-06-17 クァンタライフ・インコーポレーテッド 液滴によるアッセイ用の検出システム
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
US9266104B2 (en) 2011-02-11 2016-02-23 Raindance Technologies, Inc. Thermocycling device for nucleic acid amplification and methods of use
EP3859011A1 (en) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Methods for forming mixed droplets
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
CA2830443C (en) 2011-03-18 2021-11-16 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
EP2714970B1 (en) 2011-06-02 2017-04-19 Raindance Technologies, Inc. Enzyme quantification
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
CN103930886A (zh) 2011-07-13 2014-07-16 伯乐生命医学产品有限公司 使用副本的meta 分析进行真实世界误差的计算
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2014039587A1 (en) 2012-09-05 2014-03-13 Bio-Rad Laboratories, Inc. Systems and methods for stabilizing droplets

Similar Documents

Publication Publication Date Title
Chung et al. Highly-efficient single-cell capture in microfluidic array chips using differential hydrodynamic guiding structures
CN103343090B (zh) 一种集成化多功能可控细胞操纵及分析微流控芯片及应用
Selva et al. Solutal convection in confined geometries: Enhancement of colloidal transport
CN104689859B (zh) 一种微流控芯片上的微液滴内部物质交换方法
WO2014145152A3 (en) On-chip microfluidic processing of particles
CN105618167A (zh) 一种用于高通量制备液滴的离心式微流控芯片
CN102008983A (zh) 适用于微胶囊生产的微流控芯片
CN104069757B (zh) 一种双重微乳液快速制备装置
CN103386336A (zh) 一种用于生产浓度梯度微液滴的微流控芯片
Lagus et al. High throughput single-cell and multiple-cell micro-encapsulation
Chiang et al. Polydimethylsiloxane-polycarbonate microfluidic devices for cell migration studies under perpendicular chemical and oxygen gradients
CN204448036U (zh) 一种微流控芯片
Wang et al. Pressure-driven phase separation based on modified porous mesh for liquid management in microgravity
CN104148124B (zh) 一种用于微流控芯片的液滴生成装置
CN106669513A (zh) 浓度梯度生成装置及一步生成浓度梯度的方法
CN104084245A (zh) 一种用于形成浓度梯度的装置及系统
Xiong et al. Oil-Triggered and Template-Confined dewetting for facile and Low-Loss sample digitization
CN105441309B (zh) 循环式单细胞捕获转移芯片
GB2543617A (en) Microfluidic arrangements
Lee et al. One‐Way Particle Transport Using Oscillatory Flow in Asymmetric Traps
CN203079946U (zh) 一种固定细胞的微流控芯片
CN101613075B (zh) 一种限制液滴运动虚拟通道的构造方法
Kukhtevich et al. A microfluidic chip with hydrodynamic traps for in vitro microscopic investigations of single cells
Dressler et al. Continuous and low error-rate passive synchronization of pre-formed droplets
CN104549589B (zh) 一种三基片微球筛选芯片及使用方法