ES2536194T3 - Métodos y composiciones para producir hidrocarburos - Google Patents

Métodos y composiciones para producir hidrocarburos Download PDF

Info

Publication number
ES2536194T3
ES2536194T3 ES09747777.2T ES09747777T ES2536194T3 ES 2536194 T3 ES2536194 T3 ES 2536194T3 ES 09747777 T ES09747777 T ES 09747777T ES 2536194 T3 ES2536194 T3 ES 2536194T3
Authority
ES
Spain
Prior art keywords
seq
synechococcus
pcc
sll0208
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES09747777.2T
Other languages
English (en)
Inventor
Andreas Schirmer
Mathew Rude
Shane Brubaker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
REG Life Sciences LLC
Original Assignee
REG Life Sciences LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by REG Life Sciences LLC filed Critical REG Life Sciences LLC
Application granted granted Critical
Publication of ES2536194T3 publication Critical patent/ES2536194T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G, C10K; LIQUEFIED PETROLEUM GAS; ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE OR UNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL; FIRELIGHTERS
    • C10L1/00—Liquid carbonaceous fuels
    • C10L1/02—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0108—Long-chain acyl-[acyl-carrier-protein] reductase (1.2.1.80)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/99—Other Carbon-Carbon Lyases (1.4.99)
    • C12Y401/99005—Octadecanal decarbonylase (4.1.99.5)
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G, C10K; LIQUEFIED PETROLEUM GAS; ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE OR UNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL; FIRELIGHTERS
    • C10L2200/00—Components of fuel compositions
    • C10L2200/04—Organic compounds
    • C10L2200/0461—Fractions defined by their origin
    • C10L2200/0469—Renewables or materials of biological origin
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G, C10K; LIQUEFIED PETROLEUM GAS; ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE OR UNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL; FIRELIGHTERS
    • C10L2290/00—Fuel preparation or upgrading, processes or apparatus therefore, comprising specific process steps or apparatus units
    • C10L2290/26—Composting, fermenting or anaerobic digestion fuel components or materials from which fuels are prepared
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02T—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO TRANSPORTATION
    • Y02T50/00—Aeronautics or air transport
    • Y02T50/60—Efficient propulsion technologies, e.g. for aircraft
    • Y02T50/678—Aviation using fuels of non-fossil origin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Método de producción de un aldehído graso o alcohol graso, comprendiendo el método (i) producir en una célula huésped un polipéptido que comprende (a) la secuencia de aminoácidos de SEQ ID NO: 66, 70, 74, 76, 78, 80 u 82, en el que el polipéptido tiene actividad reductasa, o (b) una secuencia de aminoácidos que tiene al menos el 70% de identidad con SEQ ID NO: 66, 70, 74, 76, 78, 80 u 82, en el que el polipéptido tiene actividad reductasa, o (c) la secuencia de aminoácidos definida en (b) con una o más sustituciones de aminoácidos conservativas, y (ii) aislar el aldehído graso o alcohol graso de la célula huésped, en el que la célula huésped se modifica genéticamente para expresar el polipéptido.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
imagen6
imagen7
imagen8
imagen9
imagen10
imagen11
imagen12
imagen13
imagen14
imagen15
imagen16
imagen17
E09747777
05-05-2015
de Cyanothece sp. PCC7245 (SEQ ID NO: 29), cce_0778 de Cyanothece sp. PCC7245 (SEQ ID NO: 31), YP_323043 de Anabaena variabilis ATCC29413 (Ava_2533) (SEQ ID NO: 33) e YP_170760 de Synechococcus elongatus PCC6301 (syc0050_d) (SEQ ID NO: 35). En la tabla 1 y la figura 38 se enumeran otros genes de biosíntesis de alcanos y alquenos.
5 Los genes de biosíntesis de aldehídos incluyen, por ejemplo, Synpcc7942_1594 de Synechococcus elongatus PCC7942 (SEQ ID NO: 65), sll0209 de Synechocystis sp. PCC6803 (SEQ ID NO: 67), cce_1430 de Cyanothece sp. ATCC51142 (SEQ ID NO: 69), PMM0533 de Prochlorococcus marinus subesp. pastoris cepa CCMP1986 (SEQ ID NO: 71), NP_96091 de Gloeobacter violaceus PCC7421 (gll3145) (SEQ ID NO: 73), ZP_00108837 de Nostoc punctiforme PCC73102 (Npun02004176) (SEQ ID NO: 75), YP_323044 de Anabaena variabilis ATCC29413
10 (Ava2534) (SEQ ID NO: 77), YP_170761 de Synechococcus elongatus PCC6301 (syc0051_d) (SEQ ID NO: 79) y alr5284 de Nostoc sp. PCC 7120 (SEQ ID NO: 81). En la tabla 1 y la figura 39 se enumeran otros genes de biosíntesis de aldehídos.
Usando los métodos descritos en el presente documento, pueden prepararse aldehídos, alcoholes grasos, alcanos, y alquenos usando un o más polipéptidos o genes de biosíntesis de aldehídos, alcanos y/o alquenos descritos en el
15 presente documento, o variantes de los mismos, utilizando células huésped o métodos libres de células.
Tabla 1: Homólogos de genes de biosíntesis de aldehídos y alcanos en genomas de cianobacterias.
Cianobacteria
N.º de registro de gen de biosíntesis de alcanos % de ID N.º de registro de gen de biosíntesis de aldehídos % de ID
Synechococcus elongatus PCC 7942
YP_400610 100 YP_400611 100
Synechococcus elongatus PCC 6301
YP_170760 100 YP_170761 100
Microcoleus chthonoplastes PCC 7420
EDX75019 77 EDX74978 70
Arthrospira maxima CS-328
EDZ94963 78 EDZ94968 68
Lyngbya sp. PCC 8106
ZP_01619575 77 ZP_01619574 69
Nodularia spumigena CCY9414
ZP_01628096 77 ZP_01628095 70
Trichodesmium erythraeum IMS101
YP_721979 76 YP_721978 69
Microcystis aeruginosa NIES-843
YP_001660323 75 YP_001660322 68
Microcystis aeruginosa PCC 7806
CAO90780 74 CAO90781 67
Nostoc sp. PCC 7120
NP_489323 74 NP_489324 72
Nostoc azollae 0708
EEG05692 73 EEG05693 70
Anabaena variabilis ATCC 29413
YP_323043 74 YP_323044 73
Crocosphaera watsonii WH 8501
ZP_00514700 74 ZP_00516920 67
Synechocystis sp. PCC 6803
NP_442147 72 NP_442146 68
Synechococcus sp. PCC 7335
EDX86803 73 EDX87870 67
Cyanothece sp. ATCC 51142
YP_001802195 73 YP_001802846 67
Cyanothece sp. CCY0110
ZP_01728578 72 ZP_01728620 68
Nostoc punctiforme PCC 73102
ZP_00108838 72 ZP_00108837 71
Acarynchloris marina MBIC11017
YP_001518340 71 YP_001518341 66
Cyanothece sp. PCC 7425
YP_002481151 71 YP_002481152 70
Cyanolhece sp. PCC 8801
ZP_02941459 70 ZP_02942716 69
Thermosynechococcus elongatus BP-1
NP_682103 70 NP_682102 70
Synechococcus sp. JA-2-3B’a(2-13)
YP_478639 68 YP_478638 63
Synechococcus sp.RCC307
YP_001227842 67 YP_001227841 64
Synechococcus sp. WH 7803
YP_001224377 68 YP_001224378 65
Synechococcus sp. WH 8102
NP_897829 70 NP_897828 65
Synechococcus sp. WH 7805
ZP_01123214 68 ZP_01123215 65
Synechococcus GOM 3012 tipo A marino no cultivado
ABD96376 70 ABD96375 65
Synechococcus sp. JA-3-3Ab
YP_473897 68 YP_473896 62
Synechococcus GOM 306 tipo A marino no cultivado
ABD96328 70 ABD96327 65
Synechococcus GOM 3M9 tipo A marino no cultivado
ABD96275 68 ABD96274 65
Synechococcus sp. CC9311
YP_731193 63 YP_731192 63
Synechococcus 5B2 tipo A marino no cultivado
ABB92250 69 ABB92249 64
Synechococcus sp. WH 5701
ZP_01085338 66 ZP_01085337 67
Gloeobacter violaceus PCC 7421
NP_926092 63 NP_926091 67
Synechococcus sp. RS9916
ZP_01472594 69 ZP_01472595 66
Synechococcus sp. RS9917
ZP_01079772 68 ZP_01079773 65
Synechococcus sp. CC9605
YP_381055 66 YP_381056 66
Cyanobium sp. PCC 7001
EDY39806 64 EDY38361 64
Prochlorococcus marinus cepa MIT 9303
YP_001016795 63 YP_001016797 66
Prochlorococcus marinus cepa MIT9313
NP_895059 63 NP_895058 65
Synechococcus sp. CC9902
YP_377637 66 YP_377636 65
19
imagen18
imagen19
imagen20
imagen21
imagen22
imagen23
imagen24
imagen25
imagen26
imagen27
imagen28
imagen29
imagen30
imagen31
imagen32
5
10
15
20
25
30
35
40
45
E09747777
05-05-2015
2 el hidrocarburo principal está en negrita
Los análisis genómicos produjeron dos genes que estaban presentes en las cepas productoras de alcanos. En la tabla 9 se representan los homólogos de estos genes de Synechococcus elongatus PCC7942 y son Synpcc7942_1593 (SEQ ID NO: 1) y Synpcc7942_1594 (SEQ ID NO: 65).
Tabla 9: Genes de cianobacterias productoras de alcanos
Id del gen objeto
Etiqueta de locus Registro de Genbank Nombre del gen Longitud COG Pfm InterPro Notas
637800026
Synpcc7942_1593 YP_400610 proteína 231 aa pfam02915 IPR009078 rubreritina de tipo
hipotética
IPR003251 ferritina/ribonucleótido
reductasa
637800027
Synpcc7942_1594 YP_400611 proteína hipotética 341 aa COG5322 pfam00106 IPR000408 IPR016040 IPR002198 deshidrogenasa prevista deshidrogenasa de cadena corta de unión
a NAD(P)
Ejemplo 2. La deleción de los genes sll0208 y sll0209 en Synechocystis sp. PCC6803 conduce a pérdida de biosíntesis de alcanos
Se delecionaron los genes que codifican para la supuesta descarbonilasa (sll0208; NP_442147) (SEQ ID NO: 3) y la enzima generadora de aldehídos (sll0209; NP_442146) (SEQ ID NO: 67) de Synechocystis sp. PCC6803 tal como sigue. Se amplificó aproximadamente 1 kb de ADN flanqueante en el sentido de 3’ y de 5’ usando el cebador sll0208/9-KO1 (CGCGGATCCCTTGATTCTACTGCGGCGAGT) con el cebador sll0208/9-KO2 (CACGCACCTAGGTTCACACTCCCATGGTATAACAGGGGCGTTGGACTCCTGTG) y el cebador sll0208/9-KO3 (GTTATACCATGGGAGTGTGAACCTAGGTGCGTGGCCGACAGGATAGGGCGTGT) con el cebador sll0208/9-KO4 (CGCGGATCCAACGCATCCTCACTAGTCGGG), respectivamente. Se usaron los productos de PCR en una PCR cruzada con los cebadores sll0208/9-KO1 y sll0208/9-KO4 para amplificar el casete de deleción de aproximadamente 2 kb de sll0208/sll0209, que se clonó en el sitio BamHI del vector de clonación pUC19. Entonces se amplificó un casete de resistencia a kanamicina (aph, KanR) a partir del plásmido pRL27 (Larsen et al., Arch. Microbiol. 178:193 (2002)) usando los cebadores Kan-aph-F (CATGCCATGGAAAGCCACGTTGTGTCTCAAAATCTCTG) y Kan-aph-R (CTAGTCTAGAGCGCTGAGGTCTGCCTCGTGAA), que se cortó luego con NcoIy XbaI y se clonó en los sitios NcoI y AvrII del casete de deleción de sll0208/sll0209, creando un casete de deleción de sll0208/sll0209 con inserción de KanR en pUC19. El vector que contenía el casete, que no se replica en cianobacterias, se transformó en Synechocystis sp. PCC6803 (Zang et al., 2007, J. Microbiol., vol. 45, págs. 241) y se seleccionaron transformantes (por ejemplo, integrantes cromosómicos mediante recombinación homóloga doble) en placas de agar BG-11 que contenían kanamicina 100 µg/ml en un incubador equipado con luz a 30ºC. Las colonas resistentes a kanamicina volvieron a sembrarse en estrías una vez y luego se sometieron a análisis genotípico usando PCR con cebadores de diagnóstico.
Se cultivaron los mutantes de deleción-inserción confirmados en 12 ml de medio BG11 con kanamicina 50 µg/ml durante 4 días a 30ºC en un agitador-incubador equipado con luz. Entonces se centrifugó 1 ml de caldo (1 min a
13.000 g) y se extrajeron los sedimentos celulares con 0,1 ml de metanol. Tras la extracción, se centrifugaron de nuevos las muestras y se sometieron los sobrenadantes a análisis de CG-EM tal como se describe en el ejemplo 1.
Tal como se muestra en la figura 5, las cepas de Synechocystis sp. PCC6803 en las que se delecionaron los genes sll0208 y sll0209 perdieron su capacidad para producir heptadeceno y octadecenal. Este resultado demuestra que los genes sll0208 y sll0209 en Synechocystis sp. PCC6803 y los genes ortólogos en otras cianobacterias (véase la tabla 1) son responsables de la biosíntesis de alcanos y aldehídos grasos en estos microorganismos.
Ejemplo 3. Producción de aldehídos grasos y alcoholes grasos en E. coli a través de la expresión heteróloga del orf1594 de Synechococcus elongatus PCC7942
Se amplificó el ADN genómico que codifica para el orf1594 de Synechococcus elongatus PCC7942 (YP_400611; supuesta enzima generadora de aldehídos) (SEQ ID NO: 65) y se clonó en los sitios NcoIy EcoRI del vector OP-80 (derivado de pCL1920) bajo el control del promotor Ptrc. Se transformó el constructo resultante (“OP80PCC7942_1594”) en E. coli MG1655 y se hicieron crecer las células a 37ºC en medio mínimo M9 con glucosa al 1% (p/v) como fuente de carbono y complementado con espectinomicina 100 µg/ml. Cuando el cultivo alcanzó una DO600 de 0,8-1,0, se indujo con IPTG 1 mM y se hicieron crecer las células durante 18-20 h adicionales a 37ºC. Se extrajeron las células de 0,5 ml de cultivo con 0,5 ml de acetato de etilo. Tras la sonicación durante 60 min, se centrifugó la muestra a 15.000 rpm durante 5 min. Se analizó la capa de disolvente mediante CG-EM tal como se describe en el ejemplo 1.
Tal como se muestra en la figura 6, las células de E. coli transformadas con el vector que portaba orf1594 de Synechococcus elongatus PCC7942 produjeron los siguientes aldehídos grasos y alcoholes grasos: hexadecanal, octadecenal, tetradecenol, hexadecenol, hexadecanol y octadecenol. Este resultado indica que orf1594 de PCC7942
35
imagen33
imagen34
imagen35
imagen36
imagen37
imagen38
imagen39
imagen40

Claims (1)

  1. imagen1
    imagen2
    imagen3
ES09747777.2T 2008-05-16 2009-05-18 Métodos y composiciones para producir hidrocarburos Active ES2536194T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US5395508P 2008-05-16 2008-05-16
US53955P 2008-05-16
PCT/US2009/044409 WO2009140696A2 (en) 2008-05-16 2009-05-18 Methods and compositions for producing hydrocarbons

Publications (1)

Publication Number Publication Date
ES2536194T3 true ES2536194T3 (es) 2015-05-21

Family

ID=41056736

Family Applications (4)

Application Number Title Priority Date Filing Date
ES09747776.4T Active ES2667469T3 (es) 2008-05-16 2009-05-18 Métodos y composiciones para producir hidrocarburos
ES15153942T Active ES2696773T3 (es) 2008-05-16 2009-05-18 Métodos y composiciones para producir ácidos grasos y alcoholes grasos
ES15179791.7T Active ES2667718T3 (es) 2008-05-16 2009-05-18 Métodos y composiciones para producir hidrocarburos
ES09747777.2T Active ES2536194T3 (es) 2008-05-16 2009-05-18 Métodos y composiciones para producir hidrocarburos

Family Applications Before (3)

Application Number Title Priority Date Filing Date
ES09747776.4T Active ES2667469T3 (es) 2008-05-16 2009-05-18 Métodos y composiciones para producir hidrocarburos
ES15153942T Active ES2696773T3 (es) 2008-05-16 2009-05-18 Métodos y composiciones para producir ácidos grasos y alcoholes grasos
ES15179791.7T Active ES2667718T3 (es) 2008-05-16 2009-05-18 Métodos y composiciones para producir hidrocarburos

Country Status (11)

Country Link
US (11) US8323924B2 (es)
EP (5) EP2288694B1 (es)
JP (2) JP2011520455A (es)
KR (2) KR101739511B1 (es)
CN (5) CN105112455B (es)
AU (2) AU2009246082A1 (es)
BR (2) BR122020013701B1 (es)
CA (3) CA2722442C (es)
ES (4) ES2667469T3 (es)
MX (3) MX350285B (es)
WO (2) WO2009140695A1 (es)

Families Citing this family (112)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100242345A1 (en) 2006-05-19 2010-09-30 LS9, Inc Production of fatty acids & derivatives thereof
US8110670B2 (en) * 2006-05-19 2012-02-07 Ls9, Inc. Enhanced production of fatty acid derivatives
DK2262901T3 (en) 2008-03-05 2019-01-21 Genomatica Inc ORGANISMS PRODUCING PRIMARY ALCOHOL
BR122020013701B1 (pt) 2008-05-16 2021-06-01 Genomatica, Inc Microorganismo geneticamente modificado para produção de um hidrocarboneto, método de produção de um alcano ou alceno e cultura de células
US20110146142A1 (en) * 2008-08-18 2011-06-23 Ls9, Inc. Systems and methods for production of mixed fatty esters
CA3038491A1 (en) * 2008-10-07 2010-04-15 REG Life Sciences, LLC Bacterial host cells engineered to express a carboxylic acid reductase and a thioesterase
ES2701501T3 (es) 2008-10-28 2019-02-22 Reg Life Sciences Llc Célula microbiana para producir alcoholes grasos
WO2013082309A1 (en) * 2011-11-29 2013-06-06 Kiverdi, Inc. Process for growing natural or engineered high lipid accumulating strain on crude glycerol and/or other sources of waste carbon for the production of oils, fuels, oleochemicals, and other valuable organic compounds
US9957534B2 (en) * 2008-11-06 2018-05-01 Kiverdi, Inc. Engineered CO2-fixing chemotrophic microorganisms producing carbon-based products and methods of using the same
US9879290B2 (en) * 2008-11-06 2018-01-30 Kiverdi, Inc. Industrial fatty acid engineering general system for modifying fatty acids
US20150017694A1 (en) * 2008-11-06 2015-01-15 Kiverdi, Inc. Engineered CO2-Fixing Chemotrophic Microorganisms Producing Carbon-Based Products and Methods of Using the Same
CN102325864B (zh) 2008-12-23 2016-01-27 Reg生命科学有限责任公司 硫酯酶相关的方法和组合物
CA2758301A1 (en) * 2009-04-10 2010-10-14 Ls9, Inc. Production of fatty acid derivatives
CN102459622B (zh) 2009-04-27 2020-08-18 基因组股份公司 脂肪酸酯的产生
EP2449124B1 (en) 2009-06-30 2015-05-27 Codexis, Inc. Production of fatty alcohols with fatty alcohol forming acyl-coa reductases (far)
AU2012200694B2 (en) * 2009-07-09 2012-11-01 Joule Unlimited Technologies, Inc. Methods and compositions for the recombinant biosynthesis of N-alkanes
US7794969B1 (en) 2009-07-09 2010-09-14 Joule Unlimited, Inc. Methods and compositions for the recombinant biosynthesis of n-alkanes
AU2011204785B2 (en) * 2009-07-09 2013-08-15 Joule Unlimited Technologies, Inc. Methods and compositions for the recombinant biosynthesis of N-alkanes
US7955820B1 (en) 2009-07-09 2011-06-07 Joule Unlimited, Inc. Methods and compositions for the recombinant biosynthesis of n-alkanes
CN105969710A (zh) * 2009-09-25 2016-09-28 Reg生命科学有限责任公司 脂肪酸衍生物的产生
EP2480673B1 (en) 2009-09-27 2018-05-23 OPX Biotechnologies, Inc. Method for producing 3-hydroxypropionic acid and other products
US20110124071A1 (en) * 2009-11-17 2011-05-26 LS9, Inc Methods and compositions for producing hydrocarbons
WO2011069105A2 (en) 2009-12-04 2011-06-09 Richard Allen Kohn Process for producing fermentation products and fermentation medium compositions therefor
US8530221B2 (en) 2010-01-14 2013-09-10 Ls9, Inc. Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
US8859259B2 (en) 2010-02-14 2014-10-14 Ls9, Inc. Surfactant and cleaning compositions comprising microbially produced branched fatty alcohols
AR084377A1 (es) * 2010-04-08 2013-05-15 Ls9 Inc Metodos y composiciones relacionados con enzimas de alcohol graso biosintetico
JP5723977B2 (ja) * 2010-06-10 2015-05-27 コリア アドバンスド インスティチュート オブ サイエンス アンド テクノロジィ 炭化水素生成能を有する変異微生物及びこれを利用した炭化水素の製造方法
WO2012006114A2 (en) 2010-06-28 2012-01-12 Codexis, Inc. Fatty alcohol forming acyl reductases (fars) and methods of use thereof
WO2012037285A2 (en) * 2010-09-14 2012-03-22 Joule Unlimited Technologies, Inc. Methods and compositions for targeting heterologous integral membrane proteins to the cyanobacterial plasma membrane
US8372610B2 (en) 2010-09-15 2013-02-12 Ls9, Inc. Production of odd chain fatty acid derivatives in recombinant microbial cells
US20140004580A1 (en) * 2010-12-20 2014-01-02 Matrix Genetics, Llc Modified photosynthetic microorganisms for producing lipids
US20130035513A1 (en) * 2011-01-26 2013-02-07 Ls9, Inc. Methods and compositions for enhanced production of fatty aldehydes and fatty alcohols
CA2827658A1 (en) * 2011-02-17 2012-08-23 The Procter & Gamble Company Bio-based linear alkylphenyl sulfonates
EP2678410B1 (en) 2011-02-17 2017-09-13 The Procter and Gamble Company Composiitons comprising mixtures of c10-c13 alkylphenyl sulfonates
MX2013010076A (es) 2011-03-03 2014-04-07 Tersus Pharmaceuticals Llc Composiciones y metodos que comprenden c16:1n7 palmitoleato.
WO2012129537A1 (en) 2011-03-23 2012-09-27 Joule Unlimited Technologies, Inc. Photoalkanogens with increased productivity
EP2691510A4 (en) 2011-03-31 2014-11-12 Exxonmobil Res & Eng Co METABOLISM DIRECTIONS BY TRANSCRIPTION FACTOR OVEREXPRESSION
US9334508B2 (en) 2011-06-17 2016-05-10 Invista North America S.A.R.L. Methods of producing carboxylic acids
AU2012272856A1 (en) 2011-06-22 2013-05-02 Genomatica, Inc. Microorganisms for producing 1,4-butanediol and methods related thereto
US9650653B2 (en) 2011-06-30 2017-05-16 Invista North America S.A.R.L. Bioconversion process for producing nylon-7, nylon-7,7 and polyesters
DE102011110945A1 (de) 2011-08-15 2013-02-21 Evonik Degussa Gmbh Biotechnologisches Syntheseverfahren von organischen Verbindungen mit alkIL-Genprodukt
DE112011105535B4 (de) 2011-08-15 2018-08-16 Toyota Jidosha Kabushiki Kaisha Verfahren zum Herstellen eines Alkans und rekombinanter Mikroorganismus, der in der Lage ist, das Alkan zu synthetisieren
DE102011110946A1 (de) 2011-08-15 2016-01-21 Evonik Degussa Gmbh Biotechnologisches Syntheseverfahren von omegafunktionalisierten Carbonsäuren und Carbonsäure-Estern aus einfachen Kohlenstoffquellen
CN102952818B (zh) 2011-08-26 2016-03-02 中国科学院青岛生物能源与过程研究所 用于在蓝细菌中提高脂肪醇产量的构建体和方法
US20140370563A1 (en) * 2011-08-26 2014-12-18 Brookhaven Science Associates, Llc Compositions and methods for the relief of inhibition of aldehyde decarbonylase
MX355504B (es) 2011-09-27 2018-04-19 Exxonmobil Res & Eng Co Éster de cera de proteína portadora de acil-acil sintasas.
WO2013063513A1 (en) 2011-10-27 2013-05-02 The Regents Of The University Of California Bacterial production of methyl ketones
US9102960B2 (en) 2011-12-16 2015-08-11 Invista North America S.á.r.l. Methods of producing 6-carbon chemicals via CoA-dependent carbon chain elongation associated with carbon storage
US9102958B2 (en) 2011-12-16 2015-08-11 Invista North America S.á.r.l. Methods of producing 6-carbon chemicals via CoA-dependent carbon chain elongation associated with carbon storage
CN103194418B (zh) * 2012-01-04 2014-09-03 武汉臻智生物科技有限公司 一种制备脂肪烷烃及其中间体烯烃的方法
US20130224818A1 (en) * 2012-02-24 2013-08-29 Shell Oil Company Method for preparing a hydrocarbon
US9096834B2 (en) 2012-02-24 2015-08-04 Exxonmobil Research And Engineering Company Recombinant microorganisms comprising thioesterase and lysophosphatidic acid acyltransferase genes for fatty acid production
EP2814972B1 (en) 2012-02-24 2020-04-22 ExxonMobil Research and Engineering Company Enhanced production of fatty acids and fatty acid derivatives by recombinant microorganisms
US9663799B2 (en) 2012-02-27 2017-05-30 Toyota Jidosha Kabushiki Kaisha Hydrocarbon synthase gene and use thereof
KR102353113B1 (ko) 2012-04-02 2022-01-19 게노마티카 인코포레이티드 지방산 유도체들의 개선된 생산
US9163267B2 (en) 2012-04-11 2015-10-20 REG Life Sciences, LLC Metathesis transformations of microbially-produced fatty acids and fatty acid derivatives
US9181568B2 (en) 2012-04-23 2015-11-10 Exxonmobil Research And Engineering Company Cell systems and methods for improving fatty acid synthesis by expression of dehydrogenases
DE102012207921A1 (de) 2012-05-11 2013-11-14 Evonik Industries Ag Mehrstufiges Syntheseverfahren mit Synthesegas
IN2014DN09551A (es) 2012-05-17 2015-07-17 Shell Int Research
AU2013262764B2 (en) 2012-05-17 2015-08-20 Shell Internationale Research Maatschappij B.V. Methods and systems for processing biomass material
WO2014012055A1 (en) * 2012-07-13 2014-01-16 Calysta Energy, Llc Biorefinery system, methods and compositions thereof
KR20150040359A (ko) 2012-08-10 2015-04-14 오피엑스 바이오테크놀로지스, 인크. 지방산 및 지방산 유도된 산물의 생산을 위한 미생물 및 방법
AU2013342143B2 (en) 2012-11-09 2017-08-10 Calysta, Inc. Compositions and methods for biological production of fatty acid derivatives
JP2016500261A (ja) 2012-12-12 2016-01-12 アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー 脂肪酸誘導体のａｃｐ媒介性生産方法
BR112015013413A2 (pt) 2012-12-14 2017-11-14 Invista Tech Sarl método para biosintetizar um produto e hospedeiro recombinante
US9637764B2 (en) 2012-12-31 2017-05-02 Invista North America S.A.R.L. Methods of producing 7-carbon chemicals via carbon chain elongation associated with cyclohexane carboxylate synthesis
EP2938730A2 (en) 2012-12-31 2015-11-04 Invista Technologies S.A R.L. Methods of producing 6-carbon chemicals via methyl-ester shielded carbon chain elongation
CN105026569A (zh) 2012-12-31 2015-11-04 英威达技术有限责任公司 通过丙酮酸和琥珀酸半醛羟醛缩合生产7-碳化学物的方法
US9920336B2 (en) 2012-12-31 2018-03-20 Invista North America S.A.R.L. Methods of producing 7-carbon chemicals from long chain fatty acids via oxidative cleavage
US9580731B2 (en) 2012-12-31 2017-02-28 Invista North America S.A.R.L. Methods of producing 7-carbon chemicals via c1 carbon chain elongation associated with coenzyme B synthesis
EP2938733A2 (en) 2012-12-31 2015-11-04 Invista North America S.a.r.l. Methods of producing 7-carbon chemicals via methyl-ester shielded carbon chain elongation
EP2938734A2 (en) 2012-12-31 2015-11-04 Invista Technologies S.A R.L. Methods of producing 7-carbon chemicals via aromatic compounds
MX346830B (es) 2013-01-16 2017-04-03 Reg Life Sciences Llc Acil-acp reductasa con propiedades mejoradas.
US9034629B2 (en) 2013-01-25 2015-05-19 Joule Unlimited Technologies, Inc. Recombinant synthesis of medium chain-length alkanes
CA2904406C (en) * 2013-03-07 2021-03-02 REG Life Sciences, LLC Downstream processing of fatty alcohol compositions produced by recombinant host cells
US9267158B2 (en) 2013-03-14 2016-02-23 Intrexon Corporation Biological production of multi-carbon compounds from methane
WO2014146026A1 (en) 2013-03-15 2014-09-18 Opx Biotechnologies, Inc. Bioproduction of chemicals
EP2984490B1 (en) * 2013-06-14 2020-05-20 Genomatica, Inc. Methods of producing omega-hydroxylated fatty acid derivatives
EP3022310B1 (en) 2013-07-19 2019-10-16 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
JP2016529259A (ja) 2013-08-19 2016-09-23 アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー 部分精製された廃グリセロールの製造
US9469586B2 (en) 2013-08-19 2016-10-18 REG Life Sciences, LLC Production of partially refined waste glycerol
CA2923730C (en) 2013-09-13 2023-03-14 REG Life Sciences, LLC Improved acetyl-coa carboxylase variants
KR20210134820A (ko) 2013-12-05 2021-11-10 게노마티카 인코포레이티드 지방족 아민의 미생물 생성
EP3739057A1 (en) 2014-04-10 2020-11-18 Genomatica, Inc. Semisynthetic routes to organic compounds
BR112016026461A2 (pt) 2014-05-15 2017-12-12 Invista Tech Sarl métodos de produção de produtos químicos com 6 carbonos com uso de 2,6-diaminopimelato como precursor para 2-aminopimelato
JP6034332B2 (ja) 2014-05-30 2016-11-30 トヨタ自動車株式会社 組換え微生物及び当該組換え微生物を用いた物質製造方法
JP6189793B2 (ja) 2014-05-30 2017-08-30 トヨタ自動車株式会社 組換え微生物及び当該組換え微生物を用いた物質製造方法
CN106574209B (zh) 2014-06-09 2020-02-14 斯泰潘公司 用于冷水清洗的洗涤剂
WO2015195697A1 (en) 2014-06-16 2015-12-23 REG Life Sciences, LLC Omega-hydroxylase-related fusion polypeptides with improved properties
US9920339B2 (en) 2014-06-16 2018-03-20 Invista North America S.A.R.L. Methods, reagents and cells for biosynthesizing compounds
CN106795530A (zh) 2014-06-16 2017-05-31 英威达技术有限责任公司 用于生物合成化合物的方法、试剂和细胞
BR112016029375A2 (pt) 2014-06-16 2017-10-17 Invista Tech Sarl métodos, reagentes e células para biossintetizar compostos
US9816117B2 (en) 2014-06-16 2017-11-14 Invista North America S.A.R.L. Methods, reagents and cells for biosynthesizing compounds
CA2955370A1 (en) 2014-07-18 2016-01-21 REG Life Sciences, LLC Microbial production of fatty diols
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
EP3868890A1 (en) 2015-11-18 2021-08-25 Provivi, Inc. Microorganisms for the production of insect pheromones and related compounds
WO2017101987A1 (en) 2015-12-15 2017-06-22 REG Life Sciences, LLC Omega-hydroxylase-related fusion polypeptide variants with improved properties
WO2017214133A2 (en) 2016-06-06 2017-12-14 Provivi, Inc. Semi-biosynthetic production of fatty alcohols and fatty aldehydes
SI3795576T1 (sl) 2016-09-14 2023-08-31 Genomatica, Inc. 1,3-maščobne spojine diola in njihovi glikozilirani derivati
JP6607204B2 (ja) * 2017-01-17 2019-11-20 トヨタ自動車株式会社 アルカン合成能を有する組換え微生物及びアルカンの製造方法
EP3577227A4 (en) 2017-02-02 2020-12-30 Cargill Inc. GENETICALLY MODIFIED CELLS PRODUCING C6-C10 FATTY ACID DERIVATIVES
JP2018143192A (ja) * 2017-03-08 2018-09-20 花王株式会社 脂肪族アルコールの生産方法
EP3607059A2 (en) 2017-04-03 2020-02-12 Genomatica, Inc. Thioesterase variants having improved activity for the production of medium-chain fatty acid derivatives
BR112019024258A2 (pt) 2017-05-17 2020-08-18 Provivi, Inc. Microorganismos yarrowia lipolytica recombinante e método para produzir um c6-c24 álcool graxo mono- ou poliinsaturado a partir de uma fonte endógena ou exógena de c6-c24 ácidograxo saturado
CN109207415B (zh) 2017-07-07 2022-09-16 苏州华赛生物工程技术有限公司 一种生产胞二磷胆碱的重组微生物及生产胞二磷胆碱的方法
US11541105B2 (en) 2018-06-01 2023-01-03 The Research Foundation For The State University Of New York Compositions and methods for disrupting biofilm formation and maintenance
JP2020080705A (ja) * 2018-11-22 2020-06-04 トヨタ自動車株式会社 変異型デカルボニラーゼ遺伝子、当該変異型デカルボニラーゼ遺伝子を有する組換え微生物及びアルカンの製造方法
CN109486835B (zh) * 2018-12-05 2022-02-11 中国科学院合肥物质科学研究院 一种源于蓝藻的产烷烃关键基因突变子及其应用
JP7131416B2 (ja) * 2019-02-01 2022-09-06 トヨタ自動車株式会社 変異型デカルボニラーゼ遺伝子、当該変異型デカルボニラーゼ遺伝子を有する組換え微生物及びアルカンの製造方法
JP2020141560A (ja) 2019-03-04 2020-09-10 トヨタ自動車株式会社 新規アルデヒド合成酵素遺伝子、当該遺伝子を有する組換え微生物及びこれを用いたアルカンの製造方法
WO2023178193A1 (en) 2022-03-16 2023-09-21 Genomatica, Inc. Acyl-acp thioesterase variants and uses thereof

Family Cites Families (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3149170A1 (de) 1980-12-15 1982-07-29 Institut Français du Pétrole, 92502 Rueil-Malmaison, Hauts-de-Seine Brennbare komposition, welche gasoel, methanol und einen fettsaeureester enthalten und als dieseltreibstoff brauchbar sind
US5482846A (en) 1988-08-31 1996-01-09 University Of Florida Ethanol production in Gram-positive microbes
US5000000A (en) 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
US5424202A (en) 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
US5028539A (en) 1988-08-31 1991-07-02 The University Of Florida Ethanol production using engineered mutant E. coli
US7053267B2 (en) 1990-03-16 2006-05-30 Calgene Llc Plant seed oils
EP0528881A4 (en) 1990-04-24 1993-05-26 Stratagene Methods for phenotype creation from multiple gene populations
EP0563191B2 (en) 1990-12-20 2000-01-19 E.I. Du Pont De Nemours And Company Nucleotide sequences of soybean acyl-acp thioesterase genes
CA2079113A1 (en) * 1991-02-22 1992-08-23 Calgene, Inc. Seed plant fatty acyl reductase proteins and genes
EP0700376A4 (en) 1993-05-25 1996-05-22 Auckland Uniservices Ltd NITROBENZILE MUSTARD QUARTERARY SALTS AND THEIR USE AS CYTOTOXIC AGENTS SELECTIVELY ACTING AGAINST HYPOXIA
US6583266B1 (en) 1993-08-19 2003-06-24 Genome Therapeutics Corporation Nucleic acid and amino acid sequences relating to mycobacterium tuberculosis and leprae for diagnostics and therapeutics
US5807893A (en) 1993-11-18 1998-09-15 Voelker; Toni Alois Plant thioesterases and use for modification of fatty acid composition in plant seed oils
US5602030A (en) 1994-03-28 1997-02-11 University Of Florida Research Foundation Recombinant glucose uptake system
WO1998055632A1 (en) 1997-06-05 1998-12-10 Calgene Llc FATTY ACYL-CoA: FATTY ALCOHOL ACYLTRANSFERASES
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US20080178519A1 (en) 1996-11-18 2008-07-31 Bp Oil International Limited Fuel composition
RU2212447C2 (ru) 2000-04-26 2003-09-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Штамм escherichia coli - продуцент аминокислоты (варианты) и способ получения аминокислот (варианты)
US7786355B2 (en) * 2000-11-17 2010-08-31 Metabolix, Inc. Production of medium chain length polyhydroxyalkanoates from fatty acid biosynthetic pathways
AU2002302919A1 (en) 2001-02-22 2002-10-03 Institut Pasteur Comparative mycobacterial geneomics as a tool for identifying targets for the diagnosis, prophylaxis or treatment of mycobacterioses
US6686133B2 (en) 2001-03-29 2004-02-03 Agfa-Gevaert Photothermographic materials processable at lower temperatures and recording processes therefor
US7314974B2 (en) 2002-02-21 2008-01-01 Monsanto Technology, Llc Expression of microbial proteins in plants for production of plants with improved properties
WO2003074676A2 (en) 2002-03-01 2003-09-12 Monsanto Technology Llc Wax ester synthase dna sequence, protein and uses thereof
US7056714B2 (en) * 2003-03-11 2006-06-06 University Of Iowa Research Foundation, Inc. Carboxylic acid reductase polypeptide, nucleotide sequence encoding same and methods of use
US7070958B2 (en) 2003-04-18 2006-07-04 Thrombolytic Science, Inc. Methods of making pro-urokinase mutants
US7608700B2 (en) 2004-03-08 2009-10-27 North Carolina State University Lactobacillus acidophilus nucleic acid sequences encoding stress-related proteins and uses therefor
CA2554948A1 (en) 2004-03-18 2005-09-22 Sca Hygiene Products Ab Method and device for producing a multi-ply web of flexible material, such as paper and nonwoven, and multi-ply material produced by the method
BRPI0510115B1 (pt) 2004-05-21 2022-09-27 The Regents Of The University Of California Método para produzir um isoprenóide ou precursor de isoprenóide através de uma via de mevalonato em escherichia coli e célula hospedeira geneticamente modificada que os produz
US7756833B2 (en) * 2004-09-22 2010-07-13 Microsoft Corporation Method and system for synthetic backup and restore
US7960519B2 (en) 2005-05-31 2011-06-14 Pierce Biotechnology, Inc. Extraction of cellular components with fatty acid derivatives
FR2888034B1 (fr) 2005-06-29 2010-10-08 Philippe Magnier Dispositif de prevention contre l'explosion d'un transformateur electrique
WO2007022169A1 (en) 2005-08-15 2007-02-22 Arizona Chemical Company Low sulfur tall oil fatty acid
US7482480B2 (en) * 2005-10-10 2009-01-27 Council Of Scientific & Industrial Research Process for the preparation of hydrocarbon fuel
US7998339B2 (en) * 2005-12-12 2011-08-16 Neste Oil Oyj Process for producing a hydrocarbon component
AU2006325187B2 (en) * 2005-12-12 2010-05-13 Neste Oil Oyj Base oil
US8535916B2 (en) 2006-02-13 2013-09-17 Ls9, Inc. Modified microorganisms and uses therefor
ITTO20060239A1 (it) 2006-03-30 2007-09-30 Rotthapharm S P A Formulazione per somministrazione orale con effetti salutistici sull'apparato cardiovascolare
US8110670B2 (en) 2006-05-19 2012-02-07 Ls9, Inc. Enhanced production of fatty acid derivatives
US20100242345A1 (en) * 2006-05-19 2010-09-30 LS9, Inc Production of fatty acids & derivatives thereof
CN101490241A (zh) * 2006-05-19 2009-07-22 Ls9公司 脂肪酸及其衍生物的制备
EP2157170B1 (en) * 2006-05-19 2014-01-15 LS9, Inc. Production of fatty acids and derivatives thereof
WO2007142784A1 (en) 2006-05-31 2007-12-13 Archer-Daniels-Midland Company Enzymatic method of making aldehydes from fatty acids
DE102006054324A1 (de) 2006-11-17 2008-05-21 Robert Bosch Gmbh Verfahren zur bildbasierten Vermessung
WO2008113041A2 (en) 2007-03-14 2008-09-18 Ls9, Inc. Process for producing low molecular weight hydrocarbons from renewable resources
CN106987532B (zh) * 2007-03-28 2021-06-08 基因组股份公司 提高脂肪酸衍生物的产量
MY154965A (en) * 2007-06-01 2015-08-28 Solazyme Inc Production of oil in microorganisms
US7897369B2 (en) 2007-08-27 2011-03-01 Regents Of The University Of Minnesota Isoprenoid wax ester synthases, isoprenoid acyl CoA-synthetases, and uses thereof
AU2008304180B2 (en) * 2007-09-27 2014-02-27 Genomatica, Inc. Reduction of the toxic effect of impurities from raw materials by extractive fermentation
TWI351779B (en) 2007-12-03 2011-11-01 Advance Smart Ind Ltd Apparatus and method for correcting residual capac
WO2009085278A1 (en) 2007-12-21 2009-07-09 Ls9, Inc. Methods and compositions for producing olefins
DK2262901T3 (en) 2008-03-05 2019-01-21 Genomatica Inc ORGANISMS PRODUCING PRIMARY ALCOHOL
BR122020013701B1 (pt) 2008-05-16 2021-06-01 Genomatica, Inc Microorganismo geneticamente modificado para produção de um hidrocarboneto, método de produção de um alcano ou alceno e cultura de células
US8232924B2 (en) 2008-05-23 2012-07-31 Alliant Techsystems Inc. Broadband patch antenna and antenna system
WO2010021711A1 (en) 2008-08-18 2010-02-25 Ls9, Inc. Systems and methods for the production of mixed fatty esters
CA3038491A1 (en) 2008-10-07 2010-04-15 REG Life Sciences, LLC Bacterial host cells engineered to express a carboxylic acid reductase and a thioesterase
ES2701501T3 (es) 2008-10-28 2019-02-22 Reg Life Sciences Llc Célula microbiana para producir alcoholes grasos
CN102325864B (zh) 2008-12-23 2016-01-27 Reg生命科学有限责任公司 硫酯酶相关的方法和组合物
WO2010118410A1 (en) 2009-04-10 2010-10-14 Ls9, Inc. Production of fatty acid derivatives
CA2758301A1 (en) 2009-04-10 2010-10-14 Ls9, Inc. Production of fatty acid derivatives
CN102459622B (zh) 2009-04-27 2020-08-18 基因组股份公司 脂肪酸酯的产生
WO2010127318A2 (en) 2009-05-01 2010-11-04 The Regents Of The University Of California Product of fatty acid esters from biomass polymers
US7955820B1 (en) * 2009-07-09 2011-06-07 Joule Unlimited, Inc. Methods and compositions for the recombinant biosynthesis of n-alkanes
US7794969B1 (en) * 2009-07-09 2010-09-14 Joule Unlimited, Inc. Methods and compositions for the recombinant biosynthesis of n-alkanes
CN105969710A (zh) 2009-09-25 2016-09-28 Reg生命科学有限责任公司 脂肪酸衍生物的产生
US20110124071A1 (en) 2009-11-17 2011-05-26 LS9, Inc Methods and compositions for producing hydrocarbons
US8530221B2 (en) 2010-01-14 2013-09-10 Ls9, Inc. Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
US8859259B2 (en) 2010-02-14 2014-10-14 Ls9, Inc. Surfactant and cleaning compositions comprising microbially produced branched fatty alcohols
US8372610B2 (en) 2010-09-15 2013-02-12 Ls9, Inc. Production of odd chain fatty acid derivatives in recombinant microbial cells
US20130084608A1 (en) 2011-09-30 2013-04-04 Codexis, Inc. Fungal proteases

Also Published As

Publication number Publication date
MX2010012192A (es) 2011-04-26
CN102027109B (zh) 2016-01-06
US20220259623A1 (en) 2022-08-18
EP2288694B1 (en) 2018-03-07
EP2998393A1 (en) 2016-03-23
KR20110019745A (ko) 2011-02-28
US8268599B2 (en) 2012-09-18
MX343063B (es) 2016-10-21
ES2667718T3 (es) 2018-05-14
US11186854B2 (en) 2021-11-30
US8658404B2 (en) 2014-02-25
EP3483265A1 (en) 2019-05-15
US20210040514A1 (en) 2021-02-11
US10563231B2 (en) 2020-02-18
WO2009140696A3 (en) 2010-03-25
WO2009140695A1 (en) 2009-11-19
CN105112454A (zh) 2015-12-02
CA2722442A1 (en) 2009-11-19
US9481899B2 (en) 2016-11-01
EP2283121A2 (en) 2011-02-16
KR101735549B1 (ko) 2017-05-16
CA2722442C (en) 2019-02-12
US20100221798A1 (en) 2010-09-02
ES2696773T3 (es) 2019-01-17
BRPI0912684A2 (pt) 2019-09-10
US20120282663A1 (en) 2012-11-08
CA2722441C (en) 2021-01-26
EP2288694A1 (en) 2011-03-02
CA2722441A1 (en) 2009-11-19
US10150975B2 (en) 2018-12-11
US20170240927A1 (en) 2017-08-24
EP3483265B1 (en) 2021-07-21
US9670512B2 (en) 2017-06-06
MX2010012197A (es) 2010-12-06
WO2009140696A2 (en) 2009-11-19
US20180080053A1 (en) 2018-03-22
ES2667469T3 (es) 2018-05-11
US20150315617A1 (en) 2015-11-05
KR20110019744A (ko) 2011-02-28
US20150044749A1 (en) 2015-02-12
US8323924B2 (en) 2012-12-04
EP2998393B1 (en) 2018-03-07
AU2009246082A1 (en) 2009-11-19
US20100249470A1 (en) 2010-09-30
CN105483068A (zh) 2016-04-13
CN102027109A (zh) 2011-04-20
BRPI0912690A2 (pt) 2012-04-17
KR101739511B1 (ko) 2017-05-24
US20130029395A1 (en) 2013-01-31
EP2927315B1 (en) 2018-09-19
US20190360009A1 (en) 2019-11-28
BR122020013701B1 (pt) 2021-06-01
AU2009246083A1 (en) 2009-11-19
US8846371B2 (en) 2014-09-30
JP2011520455A (ja) 2011-07-21
CN105112455B (zh) 2016-08-17
CN105112455A (zh) 2015-12-02
CN102027108A (zh) 2011-04-20
WO2009140695A8 (en) 2011-04-07
MX350285B (es) 2017-09-04
EP2283121B1 (en) 2015-02-11
EP2927315A1 (en) 2015-10-07
JP2011522525A (ja) 2011-08-04
CA3101888A1 (en) 2009-11-19
CN105112454B (zh) 2017-01-18

Similar Documents

Publication Publication Date Title
ES2536194T3 (es) Métodos y composiciones para producir hidrocarburos
JP5291673B2 (ja) 遺伝子工学のためのプロモーターおよびプラスミドシステム
CA2969379C (fr) Procede de production d'au moins un metabolite d'interet par transformation d'un pentose dans un microorganisme
EP2367949B1 (en) Production of branched-chain alcohols by photosynthetic microoraganisms
US9506087B2 (en) Glucose and xylose co-utilization in E. coli
WO2016162712A1 (en) Modified microorganism for the optimized production of 2,4-dihydroxyburyrate
BRPI0808972A2 (pt) Novos microorganismos para a produção de 1,2-propanodiol obtidos através de uma combinação de evolução e design racional
EP3050970A1 (en) Modified microorganism for optimized production of 1,4-butanediol
US20150104854A1 (en) Metabolically engineered methanotrophic, phototrophic microorganisms
US20230183627A1 (en) Biomanufacturing systems and methods for producing organic products from recombinant microorganisms
US20150240247A1 (en) Trophic conversion of photoautotrophic bacteria for improved diurnal properties
KR20210128742A (ko) 글리세롤 생성이 억제된 재조합 내산성 효모 및 이를 이용한 젖산의 제조방법
US10392634B2 (en) Method for high-efficiency production of pinoresinol using an H2O2 auto-scavenging cascade
EP2524035A2 (en) Constructs, vectors and cyanobacteria for the synthesis of fatty alcohols, and methods for producing fatty alcohols in cyanobacteria
Saliola et al. Two genes encoding putative mitochondrial alcohol dehydrogenases are present in the yeast Kluyveromyces lactis
US10308915B2 (en) Genes and uses thereof, methods for synthesizing odd numbered medium chain aliphatic aldehydes and methods for synthesizing even numbered medium chain aliphatic hydrocarbons
KR102173766B1 (ko) 메틸영양세균을 이용한 1,2-프로필렌글라이콜 생산용 조성물 및 그의 생산 방법
KR20210038805A (ko) 1,2-프로필렌글라이콜 생산성이 증강된 메틸영양세균 및 이를 이용한 1,2-프로필렌글라이콜 생산방법
KR20140032057A (ko) 글리세롤 탈수 반응 산물의 생산 능력이 향상된 재조합 미생물 및 그 용도
KR102306725B1 (ko) 아세토인 생산능을 갖는 유전적으로 조작된 효모 및 이를 이용한 아세토인 생산방법
KR102603304B1 (ko) 포피라-334 생산용 조성물 및 생산방법
US10894955B2 (en) Mutant decarbonylase gene, recombinant microorganism comprising the mutant decarbonylase gene, and method for producing alkane
BR112016014779B1 (pt) Organismos microbianos que não ocorrem naturalmente, e método de produção de um composto bioderivado