EP1313738B1 - Process for the preparation and purification of thiol-containing maytansinoids - Google Patents

Process for the preparation and purification of thiol-containing maytansinoids Download PDF

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EP1313738B1
EP1313738B1 EP01926594A EP01926594A EP1313738B1 EP 1313738 B1 EP1313738 B1 EP 1313738B1 EP 01926594 A EP01926594 A EP 01926594A EP 01926594 A EP01926594 A EP 01926594A EP 1313738 B1 EP1313738 B1 EP 1313738B1
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maytansinol
methyl
maytansinoid
thiol
alanine
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EP1313738A4 (en
EP1313738A1 (en
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Ravi Vankeepuram Jagannatha Chari
Wayne Charles Widdison
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Immunogen Inc
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Immunogen Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D498/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D498/12—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms in which the condensed system contains three hetero rings
    • C07D498/18—Bridged systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
    • A61K47/6803—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates
    • A61K47/68033—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates the drug being a maytansine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Definitions

  • the present invention relates to a process for preparing and purifying cytotoxic agents. More specifically, the invention relates a process for preparing and purifying cytotoxic agents comprising thiol-containing maytansinoids. These cytotoxic agents can be used as therapeutic agents by linking them to a cell binding agent, through the thiol group, and then delivering them to a specific cell population in a targeted fashion.
  • Cytotoxic drugs such as methotrexate, daunorubicin, doxorubicin, vincristine, vinblastine, melphalan, mitomycin C, and chlorambucil have been conjugated to a variety of murine monoclonal antibodies.
  • the drug molecules were linked to the antibody molecules through an intermediary carrier molecule such as serum albumin (Garnett et al., 46 Cancer Res. 2407-2412 (1986); Ohkawa et al., 23 Cancer Immumol.
  • One of the cleavable linkers that has been employed for the preparation of antibody-drug conjugates is an acid-labile linker based on cis-aconitic acid that takes advantage of the acidic environment of different intracellular compartments such as the endosomes encountered during receptor mediated endocytosis and the lysosomes.
  • Shen and Ryser introduced this method for the preparation of conjugates of daunorubicin with macromolecular carriers (102 Biochem. Biophys. Res. Commun. 1048-1054 (1981)).
  • Yang and Reisfeld used the same technique to conjugate daunorubicin to an anti-melanoma antibody (80 J. Natl. Canc. Inst. 1154-1159 (1988)). Dillman et al.
  • Another major drawback with existing antibody-drug conjugates is their inability to deliver a sufficient concentration of drug to the target site because of the limited number of targeted antigens and the relatively moderate cytotoxicity of cancerostatic drugs like methotrexate, daunorubicin and vincristine.
  • cancerostatic drugs like methotrexate, daunorubicin and vincristine.
  • an antibody conjugate of doxorubicin was evaluated in human clinical trials, and found to be ineffective (Tolcher et al., 17 J. Clinical Oncol. 478-484 (1999)).
  • linkage of a large number of drug molecules either directly to the antibody or through a polymeric carrier molecule becomes necessary.
  • such heavily modified antibodies often display impaired binding to the target antigen and fast in vivo clearance from the blood stream.
  • Maytansinoids are highly cytotoxic drugs. Maytansine was first isolated by Kupchan et al. from the east African shrub Maytenus serrata and shown to be 100 to 1000 fold more cytotoxic than conventional cancer chemotherapeutic agents like methotrexate, daunorubicin, and vincristine (U.S. Pat. No. 3,896,111). Subsequently it was discovered that some microbes also produce maytansinoids, such as maytansinol and C-3 esters of maytansinol (U.S. Pat. No. 4,151,042). Synthetic C-3 esters of maytansinol and analogues of maytansinol have also been reported (Kupchan et al., 21 J. Med.
  • Examples of analogues of maytansinol from which C-3 esters have been prepared include maytansinol with modifications on the aromatic ring (e.g. dechloro) or at the C-9, C-14 (e.g. hydroxylated methyl group), C-15, C-18, C-20 and C-4,5.
  • the naturally occurring and synthetic C-3 esters can be classified into two groups:
  • Esters of group (b) were found to be much more cytotoxic than esters of group (a).
  • Maytansine is a mitotic inhibitor. Treatment of L1210 cells in vivo with maytansine has been reported to result in 67% of the cells accumulating in mitosis. Untreated control cells were reported to demonstrate a mitotic index ranging from between 3.2 to 5.8% (Sieber et al., 43 Comparative Leukemia Research 1975, Bibl. Haemat. 495-500 (1976)). Experiments with sea urchin eggs and clam eggs have suggested that maytansine inhibits mitosis by interfering with the formation of microtubules through the inhibition of the polymerization of the microtubule protein, tubulin (Remillard et al., 189 Science 1002-1005 (1975)).
  • maytansine has also been shown to be active. Tumor growth in the P388 lymphocytic leukemia system was shown to be inhibited over a 50- to 100-fold dosage range which suggested a high therapeutic index; also significant inhibitory activity could be demonstrated with the L1210 mouse leukemia system, the human Lewis lung carcinoma system and the human B-16 melanocarcinoma system (Kupchan, 33 Ped. Proc. 2288-2295 (1974)).
  • the maytansinoids are highly cytotoxic, they were expected to be of use in the treatment of many diseases such as cancer. This expectation has yet to be realized. Clinical trials with maytansine were not favorable due to a number of side effects (Issel et al., 5 Can. Trtmnt. Rev. 199-207 (1978)). Adverse effects to the central nervous system and gastrointestinal symptoms were responsible for some patients refusing further therapy (Issel at 204), and it appeared that maytansine was associated with peripheral neuropathy that might be cumulative (Issel at 207).
  • a further drawback to the therapeutic use of maytansinoids, of interest here, is that the process for the preparation and purification of thiol-containing maytansinoids involves several inefficient chromatographic steps that are cumbersome, not easily scalable and result in only moderate yields.
  • U.S. Pat. Nos. 5,208,020 and 5,416,064 disclose that a thiol-containing maytansinoid may be produced by first converting a maytansinoid bearing an ester group into maytansinol, then esterifying the resulting maytansinol with N -methyl- L -alanine or N -methyl- L -cysteine derivatives to yield disulfide-containing maytansinoids, followed by cleavage of the disulfide group with dithiothreitol to the thiol-containing maytansinoids.
  • this process involves several inefficient steps that are cumbersome and result in moderate yields.
  • maytansinol is first derived from maytansine or other esters of maytansinol by reduction, such as with lithium aluminum hydride.
  • maytansinol is first derived from maytansine or other esters of maytansinol by reduction, such as with lithium aluminum hydride.
  • maytansinol is also possible to isolate maytansinol from the microorganism Nocardia (see Higashide et al., U.S. Pat. No. 4,151,042).
  • the conversion of Ansamitocin P-3 into maytansinol by reductive hydrolysis with lithium aluminum hydride in tetrahydrofuran at -5°C was described in U.S. Pat. No. 4,162,940.
  • the reaction with lithium aluminum hydride results in the formation of several side products which can be removed only by careful preparative thin layer chromatography on silica gel. Thus, this process is not amenable for industrial scale use.
  • the next step in the process is the conversion of maytansinol to different ester derivatives using N -methyl- L -alanine or N -methyl- L -cysteine derivatives, and suitable agents such as dicyclohexylcarbodiimide (DCC) and catalytic amounts of zinc chloride (see U.S. Pat. No. 4,137,230; Kawai et al., 32 Chem. Pharm. Bull. 3441-3951 (1984); U.S. Pat. No. 4,260,609).
  • DCC dicyclohexylcarbodiimide
  • zinc chloride see U.S. Pat. No. 4,137,230; Kawai et al., 32 Chem. Pharm. Bull. 3441-3951 (1984); U.S. Pat. No. 4,260,609
  • the last step in the process the reduction of the disulfide-containing maytansinoids to the corresponding thiol-containing maytansinoids, is achieved by treatment with dithiothreitol (DTT), purification by HPLC using a C-18 column and elution with a linear gradient of 55% to 80% acetonitrile in H 2 O.
  • DTT dithiothreitol
  • this step results in low yields and is also not amenable for industrial scale use.
  • Thiol-containing maytansinoids are not very soluble in the ethanol/water solvent mixture used for the reaction.
  • purification by HPLC on a reverse phase C-18 column using acetonitrile/water as the mobile phase results in poor recovery and can result in the dimerization of some product.
  • the object of the present invention is to provide an improved process for the preparation and purification of thiol-containing maytansinoids that reduces the complexity of the process, allows scalability and improves the yield.
  • the present invention provides a process for preparing a thiol-containing maytansinoid comprising the steps of:
  • the reducing agent in (1) is lithium trimethoxyaluminum hydride.
  • the reducing agent in (1) is used in a concentration of from about 5 to 100 equivalents per mole of the maytansinoid C-3 ester. More preferably, the reducing agent in (1) is used in a concentration of from about 7.5 to 30 equivalents per mole of the maytansinoid C-3 ester. Most preferably, the reducing agent in (1) is used in a concentration from about 10 to 20 equivalents per mole of the maytansinoid C-3 ester.
  • the reductive hydrolysis in (1) is conducted at a temperature of from about -80°C to 0°C. More preferably, the reductive hydrolysis in (1) is conducted at a temperature of from about -45°C to -27.5°C. Most preferably, the reductive hydrolysis in (1) is conducted at a temperature of from about -35°C to -30°C.
  • the reducing agent in (1) is added over a period of from about 5 to 40 minutes. More preferably, the reducing agent in (1) is added over a period of from about 7 to 20 minutes. Most preferably, the reducing agent in (1) is added over a period of from about 8 to 12 minutes.
  • the maytansinol is purified in (2) by chromatography. More preferably, the maytansinol is purified in (2) by chromatography where the chromatography is silica gel column chromatography, preparative thin-layer chromatography on silica gel or cyano-bonded silica HPLC column chromatography. Most preferably, the maytansinol is purified in (2) by chromatography where the chromatography is silica gel column chromatography.
  • the purification in (2) is performed at ambient temperature.
  • the maytansinol in (2) is purified to a purity of about 95%.
  • the carboxylic acid in (3) is selected from the group consisting of N -methyl- N -methyldithioacetyl- L -alanine, N -methyl- N -(3-methyldithio-propanoyl)- L -alanine, N -methyl- N -(3-methyldithio-butanoyl)- L -alanine, N -methyl- N -(4-methyldithio-butanoyl)- L -alanine, N -methyl- N -(5-methyldithio-pentanoyl)- L -alanine, N -methyl- N -(3-phenyldithio-propanoyl)- L -alanine, N -methyl- N -[3-(4-nitrophenyldithio)-propanoyl] L -alanine, N -acetyl- N -methyl-methyldi
  • the esterification in (3) is conducted at ambient temperature.
  • the esterification in (3) further comprises the use of dicyclohexylcarbodiimide and zinc chloride.
  • the separating in (4) is carried out by passing the reaction mixture over a cyano-bonded silica HPLC column.
  • the separating in (4) is carried out at about 25°C.
  • the reduction in (5) uses dithiothreitol as the reducing agent.
  • the reduction in (5) is carried out in a mixture of ethyl acetate-methanol-aqueous buffer which is capable of keeping buffer salts, dithiothreitol, unreduced maytansinoids and reduced maytansinoids in solution. More preferably, the mixture of ethyl acetate-methanol-aqueous buffer is 1:1.5:1, v/v/v, ethyl acetate:methanol:aqueous buffer.
  • the concentration of the thiol-containing maytansinoid is such that the thiol-containing maytansinoid remains soluble in ethyl acetate-methanol-aqueous buffer.
  • the concentration of the thiol-containing maytansinoid is about 4 g/L.
  • the reduction in (5) is carried out in an oxygen-free atmosphere.
  • the reduction in (5) is carried out at about 25°C.
  • the purification of the thiol-containing maytansinoid in (6) is by chromatography. More preferably, the chromatography is by a cyano-bonded HPLC column. Most preferably, the chromatography is by a cyano-bonded HPLC column equilibrated and run in an organic solvent.
  • the organic solvent is a mixture of hexanes:2-propanol:ethyl acetate, more preferably the solvent is a 78.0:5.5:16.5, v/v/v, mixture of hexanes:2-propanol:ethyl acetate.
  • This invention is based on the synthesis of thiol-containing maytansinoid derivatives that retain high cytotoxicity and that can be effectively linked to cell binding agents.
  • the art reveals that the existing methods for producing thiol-containing maytansinoids are complex, non-scalable and produce low product yield.
  • the present invention overcomes these problems by disclosing a novel process for producing thiol-containing maytansinoids that reduces the complexity of the process, allows scalability and improves product yield.
  • the invention provides a novel process for the production of thiol-containing maytansinoids, useful agents for the elimination of diseased or abnormal cells that are to be killed or lysed, such as tumor cells (particularly solid tumor cells), virus infected cells, microorganism infected cells, parasite infected cells, autoimmune cells (cells that produce autoantibodies), activated cells (those involved in graft rejection or graft vs. host disease), or any other type of diseased or abnormal cells, while exhibiting a minimum of side effects.
  • thiol-containing maytansinoids can be chemically linked to a cell binding agent while keeping a high cytotoxicity either in bound form or in released form or in both states.
  • High cytotoxicity is defined as exhibiting a toxicity having an IC 50 --the inhibiting concentration of a toxic substance that leaves a surviving fraction of 0.5--of about 10 -8 M or less when measured in vitro with KB cells upon a 24 hour exposure time to the drug.
  • Cell binding agents may be of any kind presently known, or that become known and include peptides and non-peptides. Generally, these can be antibodies (especially monoclonal antibodies), lymphokines, hormones, growth factors, nutrient-transport molecules (such as transferrin), or any other cell binding molecule or substance.
  • maytansinoids include maytansinol and maytansinol analogues.
  • suitable maytansinol analogues include those having a modified aromatic ring and those having modifications at other positions.
  • Suitable analogues of maytansinol having a modified aromatic ring include:
  • a linking group is used.
  • Suitable linking groups are well known in the art and include disulfide groups, thioether groups, acid labile groups, photolabile groups, peptidase labile groups and esterase labile groups. Preferred are disulfide groups and thioether groups.
  • the linking group is part of a chemical moiety that is covalently bound to the maytansinoid through disclosed methods.
  • the chemical moiety is covalently bound to the maytansinoid via an ester linkage.
  • the linkage position is expected to be useful as the linkage position, depending upon the type of link.
  • the C-3 position having a hydroxyl group, the C-14 position modified with hydroxymethyl, the C-15 position modified with hydroxy and the C-20 position having a hydroxy group are all expected to be useful.
  • the C-3 position is preferred and the C-3 position of maytansinol is especially preferred.
  • N -methyl- L -alanine-containing C-3 ester of maytansinol and an N -methyl- L -cysteine-containing C-3 ester of maytansinol or their analogues.
  • the present invention recites a process for the preparation and purification of a thiol-containing maytansinoid that positions the linking group at C-3 of maytansinol.
  • Step 1 Synthesis of Maytansinol (2) from an Ester-Bearing Maytansinoid
  • the first step in the preparation and purification of thiol-containing maytansinoids involves the reductive hydrolysis of a maytansinoid C-3 ester into maytansinol (2).
  • the crude maytansinol (2) may be purified by dissolving it in a minimum volume of ethyl acetate, dichloro methane, ether, chloroform or toluene, more preferably ethyl acetate, and purified via silica gel column chromatography using dichloromethane, chloroform, ethyl acetate or toluene, more preferably dichloromethane.
  • the maytansinol (2) is eluted with a step gradient starting with dichloromethane or ethyl acetate, or a mixture of dichloromethane:ethyl acetate:alcohol, chloroform:ethyl acetate or toluene:ethyl acetate, preferably 77:23:0, v/v/v, dichloromethane:ethyl acetate:alcohol.
  • the concentration of alcohol is slowly increased from 0 to about 20%, preferably from 0 to about 10%.
  • Fractions containing the desired product are pooled and evaporated under reduced pressure to yield pure maytansinol (2) as a white solid.
  • the purification may be performed at ambient temperature.
  • the purification is performed at a temperature of about 20°C and 25°C.
  • the alcohol is methanol, ethanol, n-propanol, iso-propanol, n-, iso-, sec-, or tert-butanol, more preferably methanol.
  • the crude maytansinol (2) may be purified using a cyano-bonded silica HPLC column that is run in normal phase, using organic solvents in the mobile phase.
  • the crude maytansinol (2) is dissolved in ethyl acetate, ethyl acetate:isopropanol:hexane (mobile phase) or in ethyl acetate-isopropanol, preferably ethyl acetate, injected onto the column, and the appropriate peak is collected.
  • the cyano-bonded HPLC column that is used for the separation is one having cyanopropyl and cyano-di-isopropyl groups stably bonded to the silica backbone.
  • the organic solvent used in the mobile phase is hexanes:2-propanol:dichloromethane:ethyl acetate.
  • the concentration of each constituent of the organic solvent is in the range 65-75:2-4:15-20:5-10, v/v/v/v, respectively. More preferably, the concentration of each element of the organic solvent is 72:3:18:7, v/v/v/v.
  • maytansinol (2) purified by this process is at least 90% pure, more preferably at least 95% pure.
  • maytansinol (2) of less than 90% purity is used in the following steps, additional purification steps may be required.
  • the reduction reaction may yield small amounts of undesired side products, in addition to the maytansinol (2).
  • purification may be required to remove the contaminants.
  • the side products are not generated, such as where the precise times, temperatures, and amounts are established, then the purification step is not required.
  • Step 2 Synthesis of Esters of Maytansinol (2) Having a Linking Group
  • the maytansinol (2) from Step 1 is next esterified with disulfide-containing N -methyl- L -alanine derivatives 6a-g or N -methyl- L -cysteine derivatives 9a-b, in the presence of dicyclohexylcarbodiimide (DCC) and zinc chloride, to yield L- and D-aminoacyl esters 10a-g and 12a-b of maytansinol, containing a disulfide-linking group. The stereoisomers are then separated, and the L-aminoacyl ester is collected.
  • DCC dicyclohexylcarbodiimide
  • zinc chloride zinc chloride
  • the separation can be conducted using silica gel chromatography, but the process is not easily converted to an industrial scale. Separation can also be accomplished through other, less desirable, means such as HPLC using chiral columns or silica columns.
  • the cyano-bonded HPLC columns that are used for the separation are those that have cyanopropyl and cyano-di-isopropyl groups stably bonded to the silica backbone.
  • Such columns are available under the trade names DiazemTM, ZorbaxTM, MonochromTM, and KromasilTM, among others.
  • the organic solvent used in the mobile phase is hexanes:2-propanol:ethyl acetate.
  • the yield of the separation is greater than about 90% and product is at least about 95% pure, more preferably, optically pure.
  • the desired L-isomer has a retention time of about 36 to 46 min, preferably 42 min, while the D-isomer elutes at about 50 to 60 minutes, preferably 56 min.
  • the esterification is carried out at about 25°C.
  • Step 3 Synthesis of Thiol-Containing Maytansinoid 11a-g and 13a-b
  • the L-aminoacyl maytansinol esters obtained from Step 2 are dissolved in a solution comprising a solvent and an alcohol. This mixture is stirred under an inert gas atmosphere, and treated with a solution of dithiothreitol or dithioerythritol, in buffer, and containing ethylenediaminetetraacetic acid (EDTA). The progress of the reaction may be monitored by HPLC and is generally complete in 3 h, although it may vary between 1 and 24 h. The completed reaction is treated with a buffered solution containing EDTA, and then extracted. The organic layers are combined, washed and dried.
  • EDTA ethylenediaminetetraacetic acid
  • the solvent the L-aminoacyl maytansinol esters obtained from Step 2 are dissolved in is ethyl acetate, dichloromethane or ether, more preferably ethyl acetate, using from about 60 to 80 mL solvent per gram maytansinoid, preferably 72 mL/g.
  • the alcohol the L-aminoacyl maytansinol esters obtained from Step 2 are dissolved in is methanol or ethanol, more preferably methanol, using from about 90 to 120 mL alcohol per gram of maytansinoid, preferably 108 mL/g.
  • the reaction between L-aminoacyl maytansinol esters and dithiothreitol is in a mixture of ethyl acetate:methanol:aqueous buffer which is capable of keeping the buffer salts, dithiothreitol and maytansinoids (reduced and non-reduced forms) in solution. More preferably, the reaction between L-aminoacyl maytansinol esters and dithiothreitol is in a 1:5:1 mixture of ethyl acetate:methanol:aqueous buffer.
  • the concentration of L-aminoacyl maytansinol esters used in Step 3 is less than about 4 g/L such that the L-aminoacyl maytansinol esters remain solubilized.
  • the reduction reaction is carried out in an oxygen-free atmosphere.
  • the inert gas is argon, nitrogen or helium, although other gases can be used. More preferably, the inert gas is argon.
  • the reduction reaction is carried out at between 4 to 30°C, more preferably at room temperature.
  • the reaction can be carried out at lower temperatures, however, the time required for completion of the reaction is increased.
  • the solution containing the L-aminoacyl maytansinol esters is treated with dithiothreitol, dithioerythritol or a phosphine reagent such as tris (2-carboxyethyl) phosphine (TCEP), more preferably dithiothreitol, using about 2 to 3 moles reducing agent per mole maytansinoid, preferably 2.5 moles/mole, in potassium phosphate buffer, sodium phosphate buffer or triethanolamine buffer, preferably potassium phosphate buffer, the buffer at a concentration between about 20 to 100 mM, preferably 50 mM, using 60 to 80 mL of the buffered DTT per gram maytansinoid, preferably 72 mL/g, and containing from about 1 to 10 mM, preferably 2 mM, ethylenediaminetetraacetic acid (EDTA).
  • TCEP tris (2-carboxyethyl) phosphine
  • the completed reaction is treated with a 0.2 M solution of potassium phosphate buffer, sodium phosphate buffer or triethanolamine buffer, more preferably potassium phosphate buffer, using from about 120 to 160 mL buffer per gram maytansinoid, preferably 144 mL/g, and containing from about 1 to 10 mM, preferably 2 mM, EDTA.
  • the extraction is with ethyl acetate, dichloro methane, or ether, more preferably ethyl acetate, using 200 to 500 mL per gram maytansinoid, preferably 300 mL/g, repeated three times.
  • the combined organic layers are washed with a saturated sodium chloride solution, water or a saturated ammonium chloride solution, more preferably a saturated sodium chloride solution, using 40 to 100 mL per gram maytansinoid, preferably 50 mL/g, and then dried over sodium sulfate or magnesium sulfate, preferably sodium sulfate.
  • the cyano-bonded HPLC columns that are used for the separation are those that have cyanopropyl and cyano-di-isopropyl groups stably bonded to the silica backbone.
  • Such columns are available under the trade names DiazemTM, ZorbaxTM, MonochromTM, and KromasilTM, to name a few. More preferably, a Diazem preparative CN column (250 mm ⁇ 50 mm, 10 micron particle size) is used.
  • the organic solvent used in the mobile phase is hexanes:2-propanol:ethyl acetate.
  • the organic solvent used in the mobile phase is a 78.0:5.5:16.5 (v/v/v) mixture of hexanes:2-propanol:ethyl acetate.
  • the flow rate is 150 mL/min.
  • the yield of the separation is greater than 75%, and product is at least about 90% pure, more preferably, at least about 95% pure.
  • the desired thiol-containing maytansinoids 11a-d and 13a-b have a retention time of 16 min, within a range from about 14 to 18 min.
  • N-methyl-L-alanine-containing maytansinoid derivatives taught by the present invention are represented by formulas (I) - (IV): wherein: Z represents H or SR, wherein R represents a methyl, linear alkyl, branched alkyl, cyclic alkyl, simple or substituted aryl or heterocyclic group; l represents an integer of 1 to 10; and may represents a maytansinoid.
  • R 1 and R 2 which may be the same or different, represents H, CH 3 or CH 2 CH 3 ;
  • Z represents H or SR, wherein R represents methyl, linear alkyl, branched alkyl, cyclic alkyl, simple or substituted aryl, or heterocyclic; m represents 0, 1, 2 or 3; and may represents a maytansinoid.
  • Z represents H or SR, wherein R represents methyl, linear alkyl, branched alkyl, cyclic alkyl, simple or substituted aryl, or heterocyclic; p represents an integer of 3 to 8; and may represents a maytansinoid.
  • Z represents H or SR.
  • R represents methyl, linear alkyl, branched alkyl, cyclic alkyl, simple or substituted aryl or heterocyclic; q represents an integer from 1 to 10; Y represents Cl or H; and X represents H or CH 3 .
  • N -methyl- L -cysteine-containing maytansinoid derivatives useful in the present invention are represented by formulas (V) and (VI): wherein: Z represents H or SR, wherein R represents methyl, linear alkyl, branched alkyl, cyclic alkyl, simple or substituted aryl, or heterocyclic; o represents 1 or 2; q represents 0 or an integer of 1 to 10; and may represents a maytansinoid.
  • Z represents H or SR, wherein R represents methyl, linear alkyl, branched alkyl, cyclic alkyl, simple or substituted aryl or heterocyclic; o represents 1 or 2; q represents 0 or an integer of 1 to 10; Y represents Cl or H; and X represents H or CH 3 .
  • linear alkyls examples include methyl, ethyl, propyl, butyl, pentyl and hexyl.
  • branched alkyls examples include isopropyl, isobutyl, sec.-butyl, tert.-butyl, isopentyl and 1-ethyl-propyl.
  • cyclic alkyls examples include cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl.
  • Examples of simple aryls include phenyl and naphthyl.
  • substituted aryls include aryls such as those described above substituted with alkyl groups, with halogens, such as Cl, Br, F, nitro groups, amino groups, sulfonic acid groups, carboxylic acid groups, hydroxy groups and alkoxy groups.
  • heterocyclics are compounds wherein the heteroatoms are selected from O, N and S, and include pyrrollyl, pyridyl, furyl and thiophene.
  • room temperature and “ambient temperature” mean environmental temperature or uncontrolled temperature.
  • optical purity means that the purity exceeds 98%.
  • ranges are specified herein, e.g. temperature, time, concentration etc., the range includes all specific values within the range, as well as subranges falling within the broad range. Where values are stated to be “about” a specific value, or range of values, it is to be understood that statistically insignificant variations in those values are included as well.
  • 2-Mercaptoacetic acid (3a), 3-mercaptopropanoic acid (3b) and 4-mercaptobutanoic acid (3c) are commercially available.
  • 5-Mercaptopentanoic acid (3d) was prepared by a modification of a literature method (Khim et al, 37 J . Org . Chem . 2714-2720 (1972)). To a stirred solution of 5-bromopentanoic acid (1.80 g, 0.01 mol) in absolute ethanol (25 mL) was added thiourea (0.761 g, 0.01 mol) and the reaction mixture was refluxed for 6 hours. A solution of 50% aqueous NaOH (20 mL) was then added and the mixture refluxed for an additional two hours.
  • 3-Mercaptobutanoic acid (3e) A solution of the commercially available ⁇ -butyrolactone (3.0 g, 35.0 mmol) in tetrahydrofuran (20 mL) was added to di-isopropylethyl amine (10.0 g, 77.5 mmol). The reaction mixture was stirred under an argon atmosphere, and treated with thioacetic acid (3.0 g, 39.0 mmol). The reaction mixture was stirred at room temperature for 6 h. The solvent was then evaporated and the product purified by column chromatography over silica gel, eluting with ethyl:hexanes (3:1, v/v) containing 3% acetic acid.
  • the thioacetate derivative of 3e was obtained as a colorless oil (2.0 g, 35% yield).
  • the thioacetate was converted into the thiol 3e by base hydrolysis.
  • a solution of the thioacetate (1.5 g, 9.3 mmol) in methanol (6 mL) was treated with a solution of 3.3 M sodium hydroxide in water (10 mL). The reaction was stirred under an argon atmosphere. The progress of the reaction was monitored by TLC, and was judged complete in 3 h.
  • reaction mixture was acidified by addition of 1 M hydrochloric acid (40 mL), and extracted with ethyl acetate (70 mL). The organic extract was dried over sodium sulfate, filtered, and evaporated under reduced pressure to give 3-mercaptobutanoic acid (3e), which was used without further purification for the synthesis of 4e.
  • Methyldithio-acetic acid (4a) To a stirred solution of mercaptoacetic acid (3a) (3.0 g, 0.0326 mmol) in water (100 mL), cooled in an ice bath, was added methyl methanethiosulfonate (4.52 g, 0.036 mol) in absolute ethanol (40 mL). The reaction mixture was allowed to warm to room temperature and stirred overnight. The reaction mixture was then diluted with saturated, aqueous NaCl (300 mL), and extracted with diethyl ether (3 x 100 mL). The combined organic layer was washed with saturated sodium chloride (100 mL), and dried over Na 2 SO 4 , and filtered.
  • 3-Methyldithio-propanoic acid (4b) To a stirred solution of 3-mercaptopropanoic acid (3b) (5.00 g. 0.047 mol) in water (150 mL), cooled in an ice bath, was added methyl methanethiosulfonate (6.54 g. 0.052 mol) in absolute ethanol (75 mL). The reaction mixture was stirred overnight at room temperature. The mixture was then diluted with saturated, aqueous NaCl (400 mL) and extracted with ether (3 x 150 mL). The combined ether extracts were washed with saturated NaCl, dried over Na 2 SO 4 and concentrated.
  • 5-Methyldithio-pentanoic acid (4d) To a stirred solution of 5-mercaptopentanoic acid (3d) (0.500 g, 3.73 mmol) in water (20 mL) was added a solution of methyl methanethiosulfonate (0.517 g, 4.10 mmol) in absolute ethanol (5 mL) and the mixture was stirred at room temperature for 3 hours. The mixture was then diluted with aqueous, saturated NaCl (100 mL) and extracted with ethyl ether (3 x 100 mL).
  • 3-(4-Nitrophenyldithio)-propanoic acid (5b).
  • One drop of a solution of 10 N NaOH was then added to the mixture and the reaction stirred for one hour.
  • the reaction mixture was then diluted with saturated NaCl (100 mL) and extracted with ethyl acetate (3 x 75 mL).
  • N -methyl- N -methyldithioacetoyl- L -alanine (6a).
  • 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (2.99 g, 15.6 mmol) and triethylamine (1.58 g, 15.6 mmol) in dry CH 2 Cl 2 (40 mL) at 0°C was added a solution of methyldithio-acetic acid (Singh et al., 104 Anal. Biochem. 51-58 (1980) (4a) (1.66 g, 12.0 mmol) in diy CH 2 Cl 2 (20 mL).
  • N -methyl- N -(3-methyldithio-propanoyl)- L -alanine (6b).
  • 3-methyldithio-propanoic acid (4b) (1.00 g, 6.57 mmol) in dry THF (20 mL) at -10°C under argon was added isobutylchloroformate (0.897 g, 6.57 mmol) and triethylamine (0.665 g, 6.57 mmol) and the reaction mixture was stirred for 15 minutes.
  • N -methyl- N -(4-methyldithio-butanoyl)- L -alanine (6c).
  • 4-methyldithio-butanoic acid (4c) (0.200 g, 1.20 mmol) in dry THF (10 mL) at -20°C under argon was added isobutyl chloroformeta (0.164 g, 1.20 mmol) and triethylamine (0.121 g, 1.20 mmol) and the mixture was stirred for twenty minutes.
  • N -Methyl- N -(3-phenyldithio-propanoyl)- L -alanine (6e).
  • a solution of 3-phenyldithio-propanoic acid (5a) (1.8 g, 8.4 mmol) in dry THF was stirred vigorously under a nitrogen atmosphere and cooled to -15°C.
  • Isobutyl chloroformate (1.2 mL, 9.25 mmol) and triethylamine (1.29 mL, 9.25 mmol) were added and the reaction mixture was stirred at this temperature for ten minutes.
  • N-Methyl-N-(3-methyldithio-butanoyl)-L-alanine (6g).
  • a solution of 4e (3.52 g, 21.2 mmol) in tetrahydrofuran (20 mL) was cooled to - 20°C, and stirred under an argon atmosphere.
  • Isobutylchloroformate (3.48 mL, 21.2 mmol) and triethyl amine (3.7 mL, 25.4 mmol) were then added. The reaction mixture was stirred at - 20°C for 15 min.
  • Methyldithio- N -methylcysteine (8a) A solution of N -methylcysteine (7a) (Undhein, K., & Eidem, A., 23 Acta Chem. Scandinavica 3129-3133 (1970)) (1.5 g, 11.1 mmol) in H 2 O (20 mL) was stirred at room temperature, under an atmosphere of argon, and treated with a solution of methyl methanethiol sulfonate (3.0 mL, 29.2 mmol) in ethanol (10 mL). The reaction mixture was stirred at this temperature for two hours and then diluted with H 2 O (100 mL) and washed with ether (4 x 40 mL).
  • Methyldithio- N -methylhomocysteine (8b) One of ordinary skill in the art would understand how to make methyldithio- N -methylhomocysteine (8b) based on the method for making methyldithio- N methylcysteine (8a).
  • N -methylhomocysteine (7b) can be made by the method previously described for N -methylcysteine (Undhein, K., & Eidem, A., 23 Acta Chem . Scandinavica 3129-3133 (1970)).
  • N -Acetyl- N -methyl-methyldithiocysteine (9a).
  • glacial acetic acid (0.25 mL, 4.4 mmol) in dry THF (4 mL)
  • isobutylchloroformate (0.57 mL, 4.4 mmol
  • triethylamine (0.61 mL, 4.4 mmol).
  • N -acetyl- N -methyl-methyldithiohomocysteine (9b) One of ordinary skill in the art will understand how to make N -acetyl- N -methyl-methyldithiohomocysteine (8b) based on the method for making N -acetyl- N -methyl-methyldithiocysteine (9a).
  • Step 1 Reduction of Ansamitocin P-3 (1c) into Maytansinol
  • Ansanmitocin P-3 (1c) was converted into maytansinol (2) by reductive hydrolysis.
  • Ansamitocin P-3 (1c) (3.2 g, 5.0 mmol) was dissolved in anhydrous THF (80 mL), and the solution was placed under an argon atmosphere, and cooled in a dry ice-acetone bath to -40°C.
  • the crude product was dissolved in ethyl acetate and loaded onto a silica gel column packed in dichloromethane.
  • the column was eluted with a step gradient starting with a mixture of dichloromethane:ethyl acetate:methanol (77:23:0, v/v/v) and slowly increasing the concentration of methanol from 0% to 10%.
  • Fractions containing the desired product were pooled and evaporated under reduced pressure to give pure maytansinol (71% yield) as a white solid.
  • the maytansinol was further purified by HPLC as follows.
  • a KromasilTM cyano preparative HPLC column 250 mm x 50 mm, 10 micron particle size
  • a solution of the maytansinol was injected onto the column. Under these conditions, the maytansinol has a retention time of 22 min and a purity of >98%.
  • Step 2 Esterification of Maytansinol (2) with N -methyl- N -methyldithioacetoyl- L -alanine (6a)
  • the compound 10a can be more efficiently purified using a DiazemTM cyano HPLC column (250 mm x 10 mm, 10 micron particle size) equilibrated in a mixture of hexanes:2-propanol:ethyl acetate (17:2:6, v/v/v) at a flow rate of 4.7 mL/min.
  • N -methyl- N -(3-methyldithio-propanoyl)- L -alanine (6b) (5.0g, 21.5 mmol) in dry methylene chloride (80 mL) was stirred under an argon atmosphere, and treated sequentially with solutions of DCC (4.58g, 22.2 mmol) in methylene chloride (54 mL), 1M ZnCl 2 in ether (4.4 mmol), and maytansinol (2) (2.0 g, 3.5 mmol) in methylene chloride (80 mL).
  • the reaction mixture was stirred at room temperature for four hours, and then filtered.
  • a DiazemTM cyano preparative HPLC column (250 mm x 50 mm, 10 micron particle size) was equilibrated in a mixture of hexanes:2-propanol:ethyl acetate (17:2:6, v/v/v) at a flow rate of 150 mL/min.
  • a solution of the mixture of the L- and D-aminoacyl ester 10 of maytansinol (1 g in 25 mL ethyl acetate) was injected onto the column. Under these conditions, the desired L-isomer (10b) has a retention time of 42 min, while the D-isomer elutes at 56 min. Optically pure product is obtained by this method, and the recovery is >90%.
  • This material can be further purified using a DiazemTM cyano preparative HPLC column (250 mm x 50 mm, 10 micron particle size) equilibrated in a mixture of hexanes:2-propanol:ethyl acetate (17:2:6, v/v/v) at a flow rate of 150 mL/min as described above for 10b.
  • a DiazemTM cyano preparative HPLC column 250 mm x 50 mm, 10 micron particle size
  • the mixture can be further purified using a DiazemTM cyano preparative HPLC column (250 mm x 50 mm, 10 micron particle size) was equilibrated in a mixture of hexanes:2-propanol:ethyl acetate (17:2:6, v/v/v) at a flow rate of 150 mL/min as described above for (10b) .
  • the residue was purified by preparative TLC on silica gel, using 6% methanol in chloroform to give the desired L-maytansinoid ester 10g.
  • the product 10g can be more efficiently purified using a_DiazemTM cyano preparative HPLC column (250 nun x 10 mm, 10 micron particle size) equilibrated in a mixture of hexanes:2-propanol:ethyl acetate (17:2:6, v/v/v) at a flow rate of 4.70 mL/min as described above for (10b) .
  • N -Acetyl- N -methyl-methyldithiocysteine (9a) (15.6 mg, 0.07 mmol) in dry methylene chloride (0.45 mL) was stirred at room temperature under an argon atmosphere and treated sequentially with solutions of 1M ZnCl 2 in ethyl ether (0.028 mmol), DCC (17.3 mg, 0.084 mmol) in methylene chloride (0.2 mL), and maytansinol (2) (4.0 mg, 0.007 mmol) in methylene chloride (0.1 mL). The reaction mixture was stirred for three hours and then filtered and the filtrate evaporated under reduced pressure.
  • the residue can be further purified using a Diazem cyano preparative HPLC column (250 mm x 50 mm, 10 micron particle size) was equilibrated in a mixture of hexanes:2-propanol:ethyl acetate (17:2:6, v/v/v) at a flow rate of 150 mL/min as described above for (10b) .
  • the completed reaction mixture was treated with a solution of 0.2 M potassium phosphate buffer (250 mL), pH 6.0, containing 2 mM EDTA, and then extracted with ethyl acetate (3 x 600 mL). The organic layers were combined, washed with brine (100 mL) and then dried over sodium sulfate. Evaporation of the solvent gave a residue of crude thiol-containing maytansinoid 11b.
  • the crude thiol-containng maytansinoid 11b was purified by HPLC using a preparative DiazemTM cyano HPLC column (250 mm x 50 mm, 10 micron particle size) that was equilibrated in a mixture of hexanes:2-propanol:ethyl acetate (78.0:5.5:16.5, v/v/v) and ran at a flow rate of 150 mL/min.
  • Thiol-containing maytansinoid 11b eluted as peak centered at 16 min. Fractions containing the product were evaporated to give pure thiol-containing maytansinoid 11b as a white solid (76% yield with a purity of 99%).

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USRE39151E1 (en) 2006-06-27
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