DK2496694T3 - Sammensætninger til saccharificering af cellulosematerial - Google Patents

Sammensætninger til saccharificering af cellulosematerial Download PDF

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DK2496694T3
DK2496694T3 DK10778790.5T DK10778790T DK2496694T3 DK 2496694 T3 DK2496694 T3 DK 2496694T3 DK 10778790 T DK10778790 T DK 10778790T DK 2496694 T3 DK2496694 T3 DK 2496694T3
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seq
polypeptide
another aspect
mature polypeptide
activity
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DK10778790.5T
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Brett Mcbrayer
Tarana Shaghasi
Elena Vlasenko
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Novozymes Inc
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
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    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
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    • C12P19/02—Monosaccharides
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    • C12P19/12—Disaccharides
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    • C12P21/00—Preparation of peptides or proteins
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
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    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01037—Xylan 1,4-beta-xylosidase (3.2.1.37)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
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  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Processing Of Solid Wastes (AREA)
  • Peptides Or Proteins (AREA)

Claims (13)

1. Enzymsammensætning, der omfatter: (I) et polypeptid med cellobiohydrolase l-aktivitet udvalgt blandt et polypeptid, der omfatter det modne polypeptid ifølge SEQ ID NO: 2; (II) et polypeptid med cellobiohydrolase Il-aktivitet udvalgt blandt et polypeptid, der omfatter det modne polypeptid ifølge SEQ ID NO: 10; (III) et polypeptid med endoglucanase l-aktivitet udvalgt blandt et polypeptid, der omfatter det modne polypeptid ifølge SEQ ID NO: 62; (IV) et polypeptid med endoglucanase Il-aktivitet udvalgt blandt et polypeptid, der omfatter det modne polypeptid ifølge SEQ ID NO: 24; (V) et polypeptid med beta-glucosidaseaktivitet udvalgt blandt et polypeptid, der omfatter det modne polypeptid ifølge SEQ ID NO: 30; (VI) et polypeptid med cellulolytisk forøgende aktivitet udvalgt blandt et polypeptid, der omfatter det modne polypeptid ifølge SEQ ID NO: 34; og et polypeptid, der omfatter det modne polypeptid ifølge SEQ ID NO: 36; og (Vil) et polypeptid med xylanaseaktivitet udvalgt blandt et polypeptid, der omfatter det modne polypeptid ifølge SEQ ID NO: 56.
2. Enzymsammensætning ifølge krav 1, der yderligere omfatter en beta-xylosidase.
3. Enzymsammensætning ifølge et hvilket som helst af kravene 1-2, der yderligere omfatter et eller flere (adskillige) enzymer udvalgt fra gruppen, der består af en hemicellulase, en carbohydratesterase, en pectinase, en protease, en catalase, en laccase, en peroxidase, et H202-producerende enzym, en oxidoreductase, et expansin, et swollenin eller en blanding deraf.
4. Enzymsammensætning ifølge et hvilket som helst af kravene 1-3, der anvendes ved en temperatur i intervallet fra ca. 40 °C til ca. 70 °C, f.eks. ca. 50 °C til ca. 65 °C, ca. 55 °C til ca. 63 °C eller ca. 55 °C til ca. 60 °C.
5. Rekombinant værtscelle, der koder for enzymsammensætningen ifølge et hvilket som helst af kravene 1-4.
6. Fremgangsmåde til fremstilling af en enzymsammensætning, der omfatter: (a) dyrkning af den rekombinante værtscelle ifølge krav 5 under betingelser, der er fremmende for produktion af enzymsammensætningen; og (b) indvinding af enzymsammensætningen.
7. Fremgangsmåde til degradering eller omdannelse af et cellulosemateriale, som omfatter: behandling af cellulosematerialet med enzymsammensætningen ifølge et hvilket som helst af kravene 1-4.
8. Fremgangsmåde ifølge krav 7, der yderligere omfatter indvinding af det degraderede cellulosemateriale.
9. Fremgangsmåde til fremstilling af et fermenteringsprodukt, der omfatter: (a) saccharificering af et cellulosemateriale med enzymsammensætningen ifølge et hvilket som helst af kravene 1-4; (b) fermentering af det saccharificerede cellulosemateriale med en eller flere (adskillige) fermenterende mikroorganismer til fremstilling af fermenteringsproduktet; og (c) indvinding af fermenteringsproduktet fra fermenteringen.
10. Fremgangsmåde til fermentering af et cellulosemateriale, der omfatter: fermentering af cellulosematerialet med en eller flere (adskillige) fermenterende mikroorganismer, hvor cellulosematerialet er saccharificeret med enzymsammensætningen ifølge et hvilket som helst af kravene 1-4.
11. Fremgangsmåde ifølge krav 10, hvor fermenteringen af cellulosematerialet frembringer et fermenteringsprodukt.
12. Fremgangsmåde ifølge krav 11, der yderligere omfatter indvinding af fermenteringsproduktet fra fermenteringen.
13. Fremgangsmåde ifølge krav 11 eller 12, hvor fermenteringsproduktet er en alkohol, en organisk syre, en keton, en aminosyre eller en gas.
DK10778790.5T 2009-11-06 2010-11-05 Sammensætninger til saccharificering af cellulosematerial DK2496694T3 (da)

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Application Number Priority Date Filing Date Title
US25901409P 2009-11-06 2009-11-06
PCT/US2010/055723 WO2011057140A1 (en) 2009-11-06 2010-11-05 Compositions for saccharification of cellulosic material

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DK19167971.1T DK3550016T3 (da) 2009-11-06 2010-11-05 Sammensætninger til saccharificering af cellulosemateriale
DK17165996.4T DK3222716T3 (da) 2009-11-06 2010-11-05 Sammensætninger til saccharificering af cellulosemateriale
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US (8) US8580536B2 (da)
EP (3) EP2496694B1 (da)
CN (2) CN106167796B (da)
BR (1) BR112012008291A8 (da)
CA (1) CA2780179A1 (da)
DK (3) DK3550016T3 (da)
MX (1) MX2012005152A (da)
WO (1) WO2011057140A1 (da)

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DK2668269T3 (da) 2011-01-26 2018-04-09 Novozymes Inc Polypeptider med endoglucanaseaktivitet og polynukleotider, som koder for dem
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