DK2622070T3 - Varianter af polypeptider med cellulolytisk forbedrende aktivitet og polynukleotider, der koder for dem - Google Patents

Varianter af polypeptider med cellulolytisk forbedrende aktivitet og polynukleotider, der koder for dem Download PDF

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DK2622070T3
DK2622070T3 DK11768246.8T DK11768246T DK2622070T3 DK 2622070 T3 DK2622070 T3 DK 2622070T3 DK 11768246 T DK11768246 T DK 11768246T DK 2622070 T3 DK2622070 T3 DK 2622070T3
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variant
parent
another aspect
determined
polypeptide
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DK11768246.8T
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Matthew Sweeney
Mark Wogulis
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Novozymes Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Claims (18)

1. Variant, som omfatter substitutionerne F179I + 1181L + I183V i det modne polypeptid ifølge SEQ ID NO: 2, hvor varianten har cellulolytisk forbedrende aktivitet, og varianten er valgt fra gruppen bestående af: (a) et polypeptid, som udviser mindst 85% sekvensidentitet med det modne polypeptid ifølge SEQ ID NO: 2; (d) et polypeptid, der kodes for af et polynukleotid, som udviser mindst 85% sekvensidentitet med det modne polypeptids kodningssekvens ifølge SEQ ID NO: 1 eller cDNA-sekvensen deraf.
2. Variant ifølge krav 1, som omfatter eller består af substitutionerne I75V + F77L + F179I + 1181L + I183V i det modne polypeptid ifølge SEQ ID NO: 2.
3. Isoleret polynukleotid, som koder for varianten ifølge krav 1 eller 2.
4. Værtscelle, som omfatter polynukleotidet ifølge krav 3.
5. Fremgangsmåde til fremstilling af en variant, hvilken fremgangsmåde omfatter: (a) dyrkning af værtscellen ifølge krav 4 under betingelser, som er egnede til ekspression af varianten; og (b) indvinding af varianten.
6. Transgen plante, plantedel eller plantecelle, som er transformeret med polynukleotidet ifølge krav 3.
7. Fremgangsmåde til fremstilling af varianten ifølge krav 1 eller 2, hvilken fremgangsmåde omfatter: (a) dyrkning af en transgen plante eller en plantecelle, som omfatter et polynukleotid, hvilket polynukleotid koder for varianten, under betingelser, der er befordrende for fremstilling af varianten; og (b) indvinding af varianten.
8. Fremgangsmåde til opnåelse af en variant, hvilken fremgangsmåde omfatter indføring i et stampolypeptid af substitutionerne F179I + 1181L + I183V i forhold til det modne polypeptid ifølge SEQ ID NO: 2, hvor varianten har cellulolytisk forbedrende aktivitet og har mindst 85% sekvensidentitet med det modne polypeptid ifølge SEQ ID NO: 2; og indvinding af varianten.
9. Fremgangsmåde til nedbrydning eller omdannelse af et celluloseholdigt materiale, hvilken fremgangsmåde omfatter: behandling af det celluloseholdige materiale med en enzymsammensætning i nærværelse af varianten ifølge krav 1 eller 2.
10. Fremgangsmåde ifølge krav 9, hvilken fremgangsmåde endvidere omfatter indvinding af det nedbrudte celluloseholdige materiale.
11. Fremgangsmåde ifølge krav 9 eller 10, hvor enzymsammensætningen omfatter et eller flere enzymer valgt fra gruppen bestående af en cellulase, et polypeptid med cellulolytisk forbedrende aktivitet, en hemicellulase, et expansin, en esterase, en laccase, et ligninolytisk enzym, en pectinase, en peroxidase, en protease og et swollenin.
12. Fremgangsmåde til fremstilling af et fermenteringsprodukt, hvilken fremgangsmåde omfatter: (a) forsukring af et celluloseholdigt materiale med en enzymsammensætning i nærværelse af varianten ifølge krav 1 eller 2; (b) fermentering af det forsukrede celluloseholdige materiale med en eller flere fermenterende mikroorganismer for at fremstille fermenteringsproduktet; og (c) indvinding af fermenteringsproduktet fra fermenteringen.
13. Fremgangsmåde til fermentering af et celluloseholdigt materiale, hvilken fremgangsmåde omfatter: fermentering af det celluloseholdige materiale med en eller flere fermenterende mikroorganismer, hvor det celluloseholdige materiale er forsukret med en enzymsammensætning i nærværelse af varianten ifølge krav 1 eller 2.
14. Fremgangsmåde ifølge krav 12 eller 13, hvor enzymsammensætningen omfatter et eller flere enzymer valgt fra gruppen bestående af en cellulase, et polypeptid med cellulolytisk forbedrende aktivitet, en hemicellulase, et expansin, en esterase, en laccase, et ligninolytisk enzym, en pectinase, en peroxidase, en protease og et swollenin.
15. Fremgangsmåde ifølge krav 13 eller 14, hvor fermenteringen af det celluloseholdige materiale frembringer et fermenteringsprodukt.
16. Fremgangsmåde ifølge krav 15, hvilken fremgangsmåde endvidere omfatter indvinding af fermenteringsproduktet fra fermenteringen.
17. Detergentsammensætning, som omfatter varianten ifølge krav 1 eller 2 og et overfladeaktivt middel.
18. Totalmedieformulering eller celledyrkningssammensætning, som omfatter varianten ifølge krav 1 eller 2.
DK11768246.8T 2010-09-30 2011-09-29 Varianter af polypeptider med cellulolytisk forbedrende aktivitet og polynukleotider, der koder for dem DK2622070T3 (da)

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US38852710P 2010-09-30 2010-09-30
PCT/US2011/054036 WO2012044836A1 (en) 2010-09-30 2011-09-29 Variants of polypeptides having cellulolytic enhancing activity and polynucleotides encoding same

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US (1) US9816082B2 (da)
EP (1) EP2622070B1 (da)
CN (2) CN107345226A (da)
BR (1) BR112013007699B1 (da)
DK (1) DK2622070T3 (da)
ES (1) ES2599613T3 (da)
MX (1) MX2013003237A (da)
WO (1) WO2012044836A1 (da)

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