DK2668270T3 - Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem - Google Patents

Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem Download PDF

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DK2668270T3
DK2668270T3 DK12716719.5T DK12716719T DK2668270T3 DK 2668270 T3 DK2668270 T3 DK 2668270T3 DK 12716719 T DK12716719 T DK 12716719T DK 2668270 T3 DK2668270 T3 DK 2668270T3
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DK12716719.5T
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Nikolaj Spodsberg
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Novozymes Inc
Novozymes As
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (10)

1. Isoleret polypeptid med cellobiohydrolaseaktivitet, som er valgt fra et polypeptid, der udviser mindst 81% sekvensidentitet med aminosyrerne 19 til 464 ifølge SEQ ID NO: 2.
2. Isoleret polypeptid med cellobiohydrolaseaktivitet, som omfatter et katalytisk domæne valgt fra et katalytisk domæne, der udviser mindst 90% sekvensidentitet med aminosyrerne 105 til 464 ifølge SEQ ID NO: 2.
3. Enzymsammensætning, som omfatter polypeptidet ifølge et hvilket som helst af kravene 1-2.
4. Isoleret polynukleotid, som koder for polypeptidet ifølge et hvilket som helst af kravene 1 -2.
5. Nukleinsyrekonstruktion, som omfatter polynukleotidet ifølge krav 4 operativt forbundet til en eller flere fremmede kontrolsekvenser, der styrer fremstillingen af polypeptidet i en ekspressionsvært.
6. Rekombinant værtscelle, som omfatter nukleinsyrekonstruktionen ifølge krav 5.
7. Fremgangsmåde til fremstilling af et polypeptid med cellobiohydrolaseaktivitet, hvilken fremgangsmåde omfatter: (a) dyrkning af værtscellen ifølge krav 6 under betingelser, som er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
8. Fremgangsmåde til nedbrydning af et celluloseholdigt materiale, hvilken fremgangsmåde omfatter: behandling af det celluloseholdige materiale med en enzymsammensætning i nærværelse af polypeptidet med cellobiohydrolaseaktivitet ifølge et hvilket som helst af kravene 1-2.
9. Fremgangsmåde ifølge krav 8, som endvidere omfatter indvinding af det nedbrudte celluloseholdige materiale.
10. Fremgangsmåde til fremstilling af et fermenteringsprodukt, hvilken fremgangsmåde omfatter: (a) forsukring af et celluloseholdigt materiale med en enzymsammensætning i nærværelse af polypeptidet med cellobiohydrolaseaktivitet ifølge et hvilket som helst af kravene 1-2; (b) fermentering af det forsukrede celluloseholdige materiale med en eller flere fermenterende mikroorganismer for at fremstille fermenteringsproduktet; og (c) indvinding af fermenteringsproduktet fra fermenteringen.
DK12716719.5T 2011-01-26 2012-01-26 Polypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem DK2668270T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP11152252 2011-01-26
EP11250698 2011-08-04
EP11191773 2011-12-02
PCT/US2012/022653 WO2012103288A1 (en) 2011-01-26 2012-01-26 Polypeptides having cellobiohydrolase activity and polynucleotides encoding same

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DK2668270T3 true DK2668270T3 (da) 2019-01-07

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US (2) US9080161B2 (da)
EP (1) EP2668270B1 (da)
CN (1) CN103517986B (da)
BR (1) BR112013018695B1 (da)
DK (1) DK2668270T3 (da)
MX (1) MX337919B (da)
WO (1) WO2012103288A1 (da)

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US9080161B2 (en) 2015-07-14
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US20130288299A1 (en) 2013-10-31
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