DE60315028T2 - Verfahren und materialien zur produktion von d-milchsäure in hefe - Google Patents
Verfahren und materialien zur produktion von d-milchsäure in hefe Download PDFInfo
- Publication number
- DE60315028T2 DE60315028T2 DE60315028T DE60315028T DE60315028T2 DE 60315028 T2 DE60315028 T2 DE 60315028T2 DE 60315028 T DE60315028 T DE 60315028T DE 60315028 T DE60315028 T DE 60315028T DE 60315028 T2 DE60315028 T2 DE 60315028T2
- Authority
- DE
- Germany
- Prior art keywords
- gene
- cell
- lactic acid
- promoter
- ldh
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C12P7/00—Preparation of oxygen-containing organic compounds
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/37—Assays involving biological materials from specific organisms or of a specific nature from fungi
- G01N2333/39—Assays involving biological materials from specific organisms or of a specific nature from fungi from yeasts
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- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
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| PCT/US2003/017310 WO2003102201A2 (en) | 2002-05-30 | 2003-05-30 | Methods and materials for the production of d-lactic acid in yeast |
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Families Citing this family (120)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1294728B1 (it) | 1997-09-12 | 1999-04-12 | Biopolo S C A R L | Ceppi di lievito per la riproduzione di acido lattico |
| US20070031950A1 (en) * | 1998-09-11 | 2007-02-08 | Winkler Aaron A | Production of D-lactic acid with yeast |
| US7141410B2 (en) * | 2000-11-22 | 2006-11-28 | Natureworks Llc | Methods and materials for the production of organic products in cells of Candida species |
| WO2003102200A2 (en) | 2002-05-30 | 2003-12-11 | Cargill Dow Llc | Fermentation process using specific oxygen uptake rates as a process control |
| PL2345662T3 (pl) | 2003-05-02 | 2016-11-30 | Genetycznie modyfikowane gatunki drożdży i sposoby fermentacji z użyciem genetycznie modyfikowanych drożdży | |
| JP4460876B2 (ja) * | 2003-11-07 | 2010-05-12 | 株式会社豊田中央研究所 | 有機酸存在下におけるプロモーター及びその利用 |
| US20050112737A1 (en) * | 2003-11-20 | 2005-05-26 | A. E. Staley Manufacturing Co. | Lactic acid producing yeast |
| JP4806904B2 (ja) * | 2004-07-09 | 2011-11-02 | トヨタ自動車株式会社 | 乳酸生産方法 |
| JP2006296377A (ja) * | 2005-04-25 | 2006-11-02 | Toyota Central Res & Dev Lab Inc | 有機酸生産用形質転換体 |
| CN101287824A (zh) | 2005-06-02 | 2008-10-15 | 卡吉尔公司 | 东方伊氏酵母种及密切相关物种的基因修饰的酵母和采用它们的发酵方法 |
| DK1760156T3 (da) | 2005-08-10 | 2013-03-18 | Univ Florida | Materialer og fremgangsmåder til effektiv mælkesyrefremstilling |
| JP4692173B2 (ja) * | 2005-09-13 | 2011-06-01 | 東レ株式会社 | Ｄ−乳酸デヒドロゲナーゼ活性を有するポリペプチド、これをコードする遺伝子およびｄ−乳酸の製造方法 |
| US7718405B2 (en) * | 2005-09-19 | 2010-05-18 | American Air Liquide, Inc. | Use of pure oxygen in viscous fermentation processes |
| WO2007038130A2 (en) * | 2005-09-22 | 2007-04-05 | Tate & Lyle Ingredients Americas, Inc. | Improved strains for the production of organic acids |
| EP1975232A4 (en) * | 2005-10-14 | 2009-07-29 | Toray Industries | YEAST AND METHOD FOR THE PRODUCTION OF L-MILKY ACID |
| WO2007117282A2 (en) | 2005-11-23 | 2007-10-18 | Natureworks Llc | Lactic acid-producing yeast cells having nonfunctional l-or d-lactate: ferricytochrome c oxidoreductase gene |
| EP1989309B1 (en) * | 2006-01-24 | 2011-08-31 | PURAC Biochem BV | Genetic modification of homolactic thermophilic bacilli |
| EP2001992B1 (en) | 2006-03-13 | 2016-07-20 | Cargill Inc. | Yeast cells having disrupted pathway from dihydroxyacetone phosphate to glycerol |
| JP5320692B2 (ja) * | 2006-06-28 | 2013-10-23 | 東レ株式会社 | 酵母及びｌ−乳酸の製造方法 |
| US20080064076A1 (en) * | 2006-09-11 | 2008-03-13 | Saucedo Victor M | Dissolved Oxygen Profile to Increase Fermentation Productivity and Economics |
| JP5141126B2 (ja) * | 2006-09-26 | 2013-02-13 | 東レ株式会社 | 連続発酵によるｄ−乳酸の製造方法 |
| WO2008052596A1 (en) * | 2006-10-31 | 2008-05-08 | Metabolic Explorer | Process for the biological production of n-butanol with high yield |
| JP2010508013A (ja) * | 2006-10-31 | 2010-03-18 | メタボリック エクスプローラー | グリセロールから１，３−プロパンジオールを高収量で生物学的に製造する方法 |
| JP2010512147A (ja) | 2006-12-07 | 2010-04-22 | マサチューセッツ インスティテュート オブ テクノロジー | 包括的転写マシナリーエンジニアリング |
| JP5130826B2 (ja) * | 2007-03-28 | 2013-01-30 | 東レ株式会社 | 連続発酵による乳酸の製造方法 |
| JP2008283917A (ja) * | 2007-05-18 | 2008-11-27 | Toray Ind Inc | 乳酸の製造方法 |
| CN101868532A (zh) | 2007-09-20 | 2010-10-20 | 阿迈瑞斯生物技术公司 | 类异戊二烯的制备 |
| ES2616428T3 (es) * | 2007-12-07 | 2017-06-13 | Toray Industries, Inc. | Casete de expresión para lactasa deshidrogenasa, levadura transformada y método para producir ácido láctico |
| JP2011507532A (ja) * | 2007-12-23 | 2011-03-10 | ジーヴォ，インコーポレイテッド | 高収率でイソブタノールを産生する酵母生物 |
| US8455239B2 (en) * | 2007-12-23 | 2013-06-04 | Gevo, Inc. | Yeast organism producing isobutanol at a high yield |
| JP2011514140A (ja) | 2007-12-27 | 2011-05-06 | ジーヴォ，インコーポレイテッド | 低濃度水溶液からの高級アルコールの回収 |
| BRPI0905928A2 (pt) * | 2008-02-04 | 2015-08-04 | Toray Industries | "método de produção de ácido lático" |
| US20090235951A1 (en) * | 2008-03-18 | 2009-09-24 | Legrande W E | Environmentally Responsible Dental Floss and Packaging |
| JP4963488B2 (ja) * | 2008-04-23 | 2012-06-27 | トヨタ自動車株式会社 | 変異体酵母及びこれを用いた物質生産方法 |
| EA018463B1 (ru) | 2008-07-08 | 2013-08-30 | ДСМ АйПи АССЕТС Б.В. | ПОЛУЧЕНИЕ ДИКАРБОНОВЫХ КИСЛОТ ПРИ НИЗКИХ pH |
| ES2401155T3 (es) | 2008-10-03 | 2013-04-17 | Metabolic Explorer | Procedimiento para la purificación de un alcohol a partir de un caldo de fermentación que utiliza un evaporador de película descendente, de película renovada, de película fina o de recorrido corto |
| US20110318794A1 (en) * | 2009-01-23 | 2011-12-29 | Agribioindustry Inc. | Method for producing d-lactic acid, and method for increasing optical purity of d-lactic acid or yield of d-lactic acid relative to sugar in lactic acid |
| EP2439271B1 (en) * | 2009-06-03 | 2013-11-20 | Toray Industries, Inc. | Polypeptide having d-lactate dehydrogenase activity, polynucleotide encoding the polypeptide, and process for production of d-lactic acid |
| KR20120104509A (ko) * | 2009-06-26 | 2012-09-21 | 게보 인코포레이티드 | 묽은 수용액으로부터 고급 알코올들의 회수 |
| SG176970A1 (en) | 2009-07-02 | 2012-02-28 | Verdezyne Inc | Biological methods for preparing adipic acid |
| EP2460882B1 (en) * | 2009-07-28 | 2017-11-29 | Mitsui Chemicals, Inc. | Method for producing d-lactic acid |
| JP5913099B2 (ja) | 2009-07-30 | 2016-04-27 | メタボリック エクスプローラー | 発酵による生化学物質生産のための変異型メチルグリオキサールシンターゼ（ｍｇｓ） |
| EP2470667B1 (en) | 2009-08-27 | 2017-11-15 | DSM IP Assets B.V. | Dicarboxylic acid fermentation process |
| MX2012004155A (es) * | 2009-10-06 | 2012-08-03 | Gevo Inc | Proceso integrado para convertir selectivamente isobutanol renovable en p-xileno. |
| EA201201449A1 (ru) | 2010-04-21 | 2013-03-29 | ДСМ АйПи АССЕТС Б.В. | Способ получения клеток, способных преобразовывать арабинозу |
| US9012190B2 (en) | 2011-06-15 | 2015-04-21 | Butamax Advanced Biofuels Llc | Use of thiamine and nicotine adenine dinucleotide for butanol production |
| US20130130339A1 (en) * | 2010-07-31 | 2013-05-23 | Myriant Corporation | Fermentation process for the production of organic acids |
| MY160995A (en) | 2010-10-13 | 2017-03-28 | Dsm Ip Assets Bv | Polypeptides with permease activity |
| MX2013005654A (es) | 2010-11-22 | 2013-07-17 | Novozymes Inc | Composiciones y metodos para la produccion de acido 3-hidroxipropionico. |
| CA2885934C (en) | 2010-11-22 | 2018-05-01 | Cargill, Incorporated | Compositions and methods for increased ethanol titer from biomass |
| US9605285B2 (en) | 2011-01-25 | 2017-03-28 | Cargill, Incorporated | Compositions and methods for succinate production |
| CN103649306B (zh) * | 2011-03-29 | 2015-07-01 | 雀巢产品技术援助有限公司 | 在d乳酸生产中有缺陷以及具有改善的免疫特征的约氏乳杆菌菌株cncm i-1225的天然衍生物 |
| EP3401322B1 (en) | 2011-04-07 | 2022-06-08 | Virdia, LLC | Lignocellulose conversion processes and products |
| EP2697252B1 (en) | 2011-04-11 | 2016-08-10 | Cargill, Incorporated | Compositions and methods for increased ethanol production from biomass |
| EA201301193A1 (ru) | 2011-04-22 | 2014-02-28 | ДСМ АйПи АССЕТС Б.В. | Дрожжевые клетки, способные к превращению сахаров, содержащих арабинозу и ксилозу |
| US8343752B2 (en) | 2011-05-03 | 2013-01-01 | Verdezyne, Inc. | Biological methods for preparing adipic acid |
| US8728798B2 (en) | 2011-05-03 | 2014-05-20 | Verdezyne, Inc. | Biological methods for preparing adipic acid |
| CN102285664B (zh) * | 2011-05-27 | 2013-06-19 | 广西崇左市湘桂糖业有限公司 | 模拟移动床分离l-乳酸和d-乳酸专用分子筛及其制备方法 |
| JP5886292B2 (ja) * | 2011-08-11 | 2016-03-16 | 三井化学株式会社 | 連続培養によるイソプロピルアルコール製造方法 |
| US9885065B2 (en) * | 2012-01-25 | 2018-02-06 | Bioamber Inc. | Methods for succinate production |
| WO2013124351A1 (en) * | 2012-02-22 | 2013-08-29 | Novozymes A/S | Advanced fermentation control |
| FR2988733B1 (fr) * | 2012-03-27 | 2016-02-05 | Carbios | Microorganisme recombinant |
| KR101438882B1 (ko) * | 2012-04-24 | 2014-09-05 | 씨제이제일제당 (주) | 신규한 ｄ형 젖산 생산균주 |
| US9493851B2 (en) | 2012-05-03 | 2016-11-15 | Virdia, Inc. | Methods for treating lignocellulosic materials |
| BR112014027474B1 (pt) | 2012-05-03 | 2020-03-17 | Virdia, Inc. | Métodos de processamento de materiais lingnocelulósicos |
| KR101576186B1 (ko) * | 2012-06-26 | 2015-12-10 | 한국생명공학연구원 | 에탄올 생산 경로가 봉쇄된 클루이베로마이세스 막시아누스 균주 및 이의 용도 |
| WO2014018757A1 (en) | 2012-07-25 | 2014-01-30 | Cargill Incorporated | Yeast cells having reductive tca pathway from pyruvate to succinate and overexpressing an exogenous nad(p)+ transhydrogenase enzyme |
| CA2884266C (en) | 2012-09-14 | 2022-05-24 | Myriant Corporation | Production of organic acids by fermentation at low ph |
| CN104718280A (zh) | 2012-10-16 | 2015-06-17 | 帝斯曼知识产权资产管理有限公司 | 具有改善的戊糖转化的细胞 |
| EP2917334A1 (en) | 2012-11-07 | 2015-09-16 | DSM IP Assets B.V. | pH CONTROLLED YEAST PROPAGATION |
| WO2014076232A2 (en) | 2012-11-19 | 2014-05-22 | Novozymes A/S | Isopropanol production by recombinant hosts using an hmg-coa intermediate |
| JP2015536669A (ja) | 2012-11-30 | 2015-12-24 | ノボザイムス，インコーポレイティド | 組換え酵母による３−ヒドロキシプロピオン酸の生産 |
| CA2914641A1 (en) | 2013-06-18 | 2014-12-24 | Calysta, Inc. | Compositions and methods for biological production of lactate from c1 compounds using lactate dehydrogenase transformants |
| US11408013B2 (en) | 2013-07-19 | 2022-08-09 | Cargill, Incorporated | Microorganisms and methods for the production of fatty acids and fatty acid derived products |
| US9845484B2 (en) | 2013-07-31 | 2017-12-19 | Novozymes A/S | 3-hydroxypropionic acid production by recombinant yeasts expressing an insect aspartate 1-decarboxylase |
| JP2017504330A (ja) | 2014-01-16 | 2017-02-09 | キャリスタ， インコーポレイテッド | 炭水化物を富化した組換え微生物 |
| KR20150096019A (ko) * | 2014-02-13 | 2015-08-24 | 삼성전자주식회사 | 유전자 조작 효율이 향상된 효모 세포 및 그의 용도 |
| US9593350B2 (en) | 2014-02-14 | 2017-03-14 | Samsung Electronics Co., Ltd. | Lactate dehydrogenase mutant, polynucleotide coding for the mutant, yeast cell including the polynucleotide, method of preparing the mutant, and method of producing the lactate using the same |
| JP6183263B2 (ja) * | 2014-03-28 | 2017-08-23 | 王子ホールディングス株式会社 | Ｄ−乳酸の精製のための工程が改良されたｄ−乳酸の製造方法 |
| EP3143075B1 (en) | 2014-05-16 | 2022-05-18 | Carbios | Process of recycling mixed pet plastic articles |
| WO2016012429A1 (en) | 2014-07-24 | 2016-01-28 | Dsm Ip Assets B.V. | Yeast cell with improved pentose transport |
| KR102245298B1 (ko) | 2014-09-05 | 2021-04-27 | 삼성전자주식회사 | 증가된 edc 활성을 갖고 락테이트 생산능을 갖는 유전적으로 조작된 효모 세포, 그를 제조하는 방법, 그를 제조하는 방법, 및 그를 사용하여 락테이트를 생산하는 방법 |
| US10214754B2 (en) | 2014-10-10 | 2019-02-26 | Jmtc Enzyme Corporation | Transformant and its production process, and method for producing lactic acid |
| CN106852154B (zh) | 2014-10-21 | 2021-07-30 | 卡比欧斯公司 | 具有聚酯降解活性的多肽及其用途 |
| WO2016081902A1 (en) | 2014-11-20 | 2016-05-26 | Full Cycle Bioplastics Inc. | Producing polyhydroxyalkanoate copolymers from organic waste products |
| EP3225691B1 (en) * | 2014-11-28 | 2020-10-28 | Ajinomoto Co., Inc. | Method for producing isoprenoid compound |
| US10626242B2 (en) | 2014-12-19 | 2020-04-21 | Carbios | Plastic compound and preparation process |
| US20170362613A1 (en) | 2014-12-19 | 2017-12-21 | Novozymes A/S | Recombinant Host Cells For The Production Of 3-Hydroxypropionic Acid |
| SE538899C2 (en) | 2015-02-03 | 2017-01-31 | Stora Enso Oyj | Method for treating lignocellulosic materials |
| WO2016138303A1 (en) | 2015-02-27 | 2016-09-01 | Novozymes A/S | Mutant host cells for the production of 3-hydroxypropionic acid |
| US10508269B2 (en) | 2015-03-13 | 2019-12-17 | Carbios | Polypeptide having a polyester degrading activity and uses thereof |
| EP3303375A1 (en) | 2015-06-04 | 2018-04-11 | BioAmber Inc. | Biobased production of functionalized alpha-substituted acrylates and c4-dicarboxylates |
| DK3307812T3 (da) | 2015-06-12 | 2024-07-08 | Carbios | Bionedbrydelig polyestersammensætning og anvendelse deraf |
| WO2017035270A1 (en) | 2015-08-24 | 2017-03-02 | Novozymes A/S | Beta-alanine aminotransferases for the production of 3-hydroxypropionic acid |
| US10717996B2 (en) | 2015-12-21 | 2020-07-21 | Carbios | Recombinant yeast cells producing polylactic acid and uses thereof |
| KR102498219B1 (ko) | 2016-05-19 | 2023-02-09 | 까르비오 | 플라스틱 제품을 분해하기 위한 방법 |
| EP3919615A1 (en) | 2016-07-13 | 2021-12-08 | DSM IP Assets B.V. | Malate dehyrogenases |
| CN110494566A (zh) | 2017-02-02 | 2019-11-22 | 嘉吉公司 | 产生c6-c10脂肪酸衍生物的经遗传修饰的细胞 |
| EP3668306A4 (en) | 2017-08-17 | 2021-06-09 | Cargill, Incorporated | GENETICALLY MODIFIED HAPLOIDISSATCHENKIA ORIENTALIS |
| US11447802B2 (en) * | 2018-02-16 | 2022-09-20 | Ptt Global Chemical Public Company Limited | Microorganisms and processes for lactic acid production |
| EP3775240A1 (en) * | 2018-03-30 | 2021-02-17 | INVISTA Textiles (U.K.) Limited | Method for controlling dissolved oxygen concentration in a continuous aerobic fermentation |
| EP3775242A1 (en) | 2018-03-30 | 2021-02-17 | INVISTA Textiles (U.K.) Limited | High hydrogen utilization and gas recycle |
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| WO2019213033A1 (en) | 2018-05-02 | 2019-11-07 | Invista North America S.A.R.L. | Materials and methods for maximizing biosynthesis through alteration of pyruvate-acetyl-coa-tca balance in species of the genera ralstonia and cupriavidus and organisms related thereto |
| EP3788156A1 (en) | 2018-05-02 | 2021-03-10 | INVISTA Textiles (U.K.) Limited | Methods for controlling limitation conditions in product biosynthesis for non-phb generating cupriavidus or ralstonia |
| TWI719317B (zh) * | 2018-06-13 | 2021-02-21 | 遠東新世紀股份有限公司 | 用於生產乳酸的方法 |
| CN109097293B (zh) * | 2018-07-13 | 2021-10-26 | 广东利世康低碳科技有限公司 | 一种能降解利用餐厨废弃物生成乳酸的基因重组毕赤酵母 |
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| EP3889256A4 (en) | 2018-11-30 | 2021-12-22 | Utilization of Carbon Dioxide Institute Co.,Ltd. | LACTIC ACID-PRODUCING TRANSFORMER OF A BACTERIA OF THE GENUS HYDROGENOPHILUS |
| CA3148422A1 (en) | 2019-08-09 | 2021-02-18 | Utilization Of Carbon Dioxide Institute Co., Ltd. | A recombinant hydrogenophilus bacterium producing lactic acid |
| WO2021034276A2 (en) * | 2019-08-19 | 2021-02-25 | Ptt Global Chemical Public Company Limited | Yeast having improvement of lactic acid tolerance and use thereof |
| TWI730383B (zh) * | 2019-08-21 | 2021-06-11 | 遠東新世紀股份有限公司 | 重組型假絲酵母菌菌株及其製備方法與用途 |
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| RU2752896C1 (ru) * | 2020-12-04 | 2021-08-11 | Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Национального исследовательского центра "Курчатовский институт" - ГосНИИгенетика) | Штамм дрожжей Schizosaccharomyces pombe, продуцирующий L-молочную кислоту, содержащий в составе хромосомы гены трех различных гетерологичных лактатдегидрогеназ |
| KR102287114B1 (ko) * | 2021-01-25 | 2021-08-06 | 씨제이제일제당 주식회사 | 신규한 사이토신 퍼미에이즈 변이체 및 이를 이용한 l-트립토판 생산 방법 |
| WO2022241027A1 (en) * | 2021-05-13 | 2022-11-17 | Lygos, Inc. | Recombinant host cells and methods for producing l-lactic acid |
| CN113430127B (zh) * | 2021-07-07 | 2024-01-12 | 江南大学 | 一种产l-乳酸的重组菌及其应用 |
Family Cites Families (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3384553A (en) * | 1965-04-08 | 1968-05-21 | Ceskoslovenska Akademie Ved | Method and equipment for aerobic fermentation on liquid culture mediums |
| IL39710A (en) | 1972-06-19 | 1975-04-25 | Imi Inst For Res & Dev | Recovery of acids from aqueous solutions by solvent extraction |
| US4064015A (en) * | 1975-05-12 | 1977-12-20 | New Brunswick Scientific Co., Inc. | Control of biosynthesis in submerged culture |
| US4771001A (en) | 1986-03-27 | 1988-09-13 | Neurex Corp. | Production of lactic acid by continuous fermentation using an inexpensive raw material and a simplified method of lactic acid purification |
| US5210296A (en) | 1990-11-19 | 1993-05-11 | E. I. Du Pont De Nemours And Company | Recovery of lactate esters and lactic acid from fermentation broth |
| US5132456A (en) | 1991-05-07 | 1992-07-21 | The Regents Of The University Of California | Sorption of carboxylic acid from carboxylic salt solutions at PHS close to or above the pKa of the acid, with regeneration with an aqueous solution of ammonia or low-molecular-weight alkylamine |
| JP3012377B2 (ja) * | 1991-10-11 | 2000-02-21 | 名糖産業株式会社 | 低耐熱性プロテアーゼとその関連物及びその生産法 |
| US5142023A (en) | 1992-01-24 | 1992-08-25 | Cargill, Incorporated | Continuous process for manufacture of lactide polymers with controlled optical purity |
| US5247059A (en) * | 1992-01-24 | 1993-09-21 | Cargill, Incorporated | Continuous process for the manufacture of a purified lactide from esters of lactic acid |
| US5420304A (en) | 1992-03-19 | 1995-05-30 | Biopak Technology, Ltd. | Method to produce cyclic esters |
| FR2693463B1 (fr) * | 1992-07-08 | 1994-09-02 | Rhone Poulenc Rorer Sa | Promoteur de levure et son utilisation. |
| US5338822A (en) | 1992-10-02 | 1994-08-16 | Cargill, Incorporated | Melt-stable lactide polymer composition and process for manufacture thereof |
| AT398982B (de) | 1993-02-18 | 1995-02-27 | Vogelbusch Gmbh | Verfahren zur abtrennung und reinigung von milchsäure |
| US5510526A (en) | 1993-06-29 | 1996-04-23 | Cargill, Incorporated | Lactic acid production, separation and/or recovery process |
| IL109003A (en) | 1994-03-16 | 1999-09-22 | Yissum Res Dev Co | Process and extractant composition for extracting water-soluble carboxylic and mineral acids |
| JPH08173170A (ja) | 1994-05-25 | 1996-07-09 | Kirin Brewery Co Ltd | キャンディダ・ユティリス酵母の形質転換系、およびそれによる異種遺伝子の発現 |
| EP0875579B1 (fr) * | 1997-05-03 | 2007-06-27 | Societe Des Produits Nestle S.A. | Production de L(+)-Lactate |
| IT1294728B1 (it) | 1997-09-12 | 1999-04-12 | Biopolo S C A R L | Ceppi di lievito per la riproduzione di acido lattico |
| US6229046B1 (en) * | 1997-10-14 | 2001-05-08 | Cargill, Incorported | Lactic acid processing methods arrangements and products |
| US6046002A (en) * | 1998-01-05 | 2000-04-04 | The Board Of Trustees Of The Leland Stanford Junior University | Highly parallel and sensitive method for identifying drugs and drug targets |
| AU1722800A (en) * | 1998-11-18 | 2000-06-05 | University Of Akron, The | Production of biological materials by simultaneous aerobic and anaerobic respiration |
| CA2374482C (en) * | 1999-05-21 | 2012-09-18 | Cargill Dow Llc | Methods and materials for the synthesis of organic products |
| US6268189B1 (en) * | 2000-03-24 | 2001-07-31 | The United States Of America As Represented By The Secretary Of Agriculture | Fungal lactate dehydrogenase gene and constructs for the expression thereof |
| JP2002136293A (ja) * | 2000-08-23 | 2002-05-14 | Toray Ind Inc | 微生物およびｄ−乳酸の製造方法 |
| DK1332222T3 (da) * | 2000-11-03 | 2009-07-06 | Genentech Inc | Stofskifteændringer i fermenteringer der udtrykker rekombinante proteiner |
| US7141410B2 (en) * | 2000-11-22 | 2006-11-28 | Natureworks Llc | Methods and materials for the production of organic products in cells of Candida species |
| US7109010B2 (en) | 2000-11-22 | 2006-09-19 | Nature Works Llc | Methods and materials for the synthesis of organic products |
| KR100426990B1 (ko) * | 2001-06-27 | 2004-04-13 | 삼성전자주식회사 | 외부의 코드에 따라 프로그래머블하게 기준 전압을 발생시키는 기준 전압 발생 회로 |
| CN100526466C (zh) * | 2002-03-11 | 2009-08-12 | 丰田自动车株式会社 | 控制乙醇产生的方法 |
| WO2003102200A2 (en) | 2002-05-30 | 2003-12-11 | Cargill Dow Llc | Fermentation process using specific oxygen uptake rates as a process control |
| EP1637603B1 (en) * | 2003-05-22 | 2010-01-27 | Toyota Jidosha Kabushiki Kaisha | Dna coding for protein having d-lactic acid dehydrogenase activity and use thereof |
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