CN1930281A - 用于通过连续灌注和交互切向流来培养细胞的方法 - Google Patents

用于通过连续灌注和交互切向流来培养细胞的方法 Download PDF

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CN1930281A
CN1930281A CNA2005800071754A CN200580007175A CN1930281A CN 1930281 A CN1930281 A CN 1930281A CN A2005800071754 A CNA2005800071754 A CN A2005800071754A CN 200580007175 A CN200580007175 A CN 200580007175A CN 1930281 A CN1930281 A CN 1930281A
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cell
cell culture
perfusion
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约翰·阔沃勒
麦可·武本恩
卓斯·曼纽尔·库库·马丁
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CNA2005800071754A 2004-03-05 2005-03-04 用于通过连续灌注和交互切向流来培养细胞的方法 Pending CN1930281A (zh)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
EP04075703.1 2004-03-05
EP04075702.3 2004-03-05
EP04075702 2004-03-05
EP04075703 2004-03-05
EP04077656 2004-09-27
EP04077656.9 2004-09-27
EP04077657 2004-09-27
EP04077657.7 2004-09-27
PCT/EP2005/002374 WO2005095578A1 (en) 2004-03-05 2005-03-04 Process for cell culturing by continuous perfusion and alternating tangential flow

Related Child Applications (2)

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CN201410471955.5A Division CN104593277A (zh) 2004-03-05 2005-03-04 用于通过连续灌注和交互切向流来培养细胞的方法
CN201410601800.9A Division CN104388324A (zh) 2004-03-05 2005-03-04 用于通过连续灌注和交互切向流来培养细胞的方法

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CN1930281A true CN1930281A (zh) 2007-03-14

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CNA2005800071754A Pending CN1930281A (zh) 2004-03-05 2005-03-04 用于通过连续灌注和交互切向流来培养细胞的方法
CN201410471955.5A Pending CN104593277A (zh) 2004-03-05 2005-03-04 用于通过连续灌注和交互切向流来培养细胞的方法

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US (3) US8206981B2 (enExample)
EP (3) EP2540815A1 (enExample)
JP (6) JP2007525984A (enExample)
KR (1) KR101050585B1 (enExample)
CN (3) CN104388324A (enExample)
AU (1) AU2005229359C1 (enExample)
CA (1) CA2557046A1 (enExample)
DK (1) DK1720972T3 (enExample)
ES (1) ES2456015T3 (enExample)
IL (2) IL177724A (enExample)
PL (1) PL1720972T3 (enExample)
PT (1) PT1720972E (enExample)
SI (1) SI1720972T1 (enExample)
WO (1) WO2005095578A1 (enExample)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103305417A (zh) * 2012-03-07 2013-09-18 无锡药明康德生物技术有限公司 进行蛋白生产的高产量反应器及其生产方法和应用
CN103525756A (zh) * 2013-10-23 2014-01-22 南京农业大学 一种原代鸡肝细胞的分离培养方法
CN106414490A (zh) * 2014-02-17 2017-02-15 爱德技术生物科学有限公司 使用基于膜的细胞截留系统增强重组蛋白的表达
CN106687575A (zh) * 2014-06-04 2017-05-17 美国安进公司 用于收获哺乳动物细胞培养物的方法
CN107532132A (zh) * 2015-02-09 2018-01-02 优尼沃赛尔斯公司 用于生产细胞和/或细胞产品的系统、装置和方法
CN109714962A (zh) * 2016-07-11 2019-05-03 耶路撒冷希伯来大学的益生研究开发有限公司 用于在体外培养细胞的系统和方法
WO2021008571A1 (zh) * 2019-07-16 2021-01-21 信达生物制药(苏州)有限公司 高密度连续接种的细胞培养方法及其应用
CN112410279A (zh) * 2013-03-15 2021-02-26 建新公司 高密度细胞存储方法
US12478571B2 (en) 2018-12-17 2025-11-25 Pontis Biologics, Inc. Enzyme based compositions and methods for removing dental calculi

Families Citing this family (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL1720972T3 (pl) 2004-03-05 2014-06-30 Dpx Holdings Bv Proces hodowli komórek poprzez ciągłą perfuzję i zmienny przepływ styczny
BRPI0707027B1 (pt) 2006-02-24 2022-08-16 Toray Industries, Inc Método de fabricação de um produto químico e aparelho de fermentação contínua
EP1870450A1 (en) * 2006-06-21 2007-12-26 DSMIP Assets B.V. Process for the culturing of E1-immortalized HER cells
AU2011211462C1 (en) * 2006-07-14 2015-01-22 Dpx Holdings B.V. Improved process for the culturing of cells
AU2007272054C1 (en) 2006-07-14 2014-08-28 Dpx Holdings B.V. Improved process for the culturing of cells
EP2014760A1 (en) 2007-06-13 2009-01-14 CMC Biopharmaceuticals A/S A method for producing a biopolymer (e.g. polypeptide) in a continuous fermentation process
KR101798813B1 (ko) * 2007-12-27 2017-11-16 박스알타 인코퍼레이티드 세포 배양 방법
WO2010003759A2 (en) * 2008-06-17 2010-01-14 Dsm Ip Assets B.V. Cell culturing method
SG190659A1 (en) 2008-09-02 2013-06-28 Pluristem Ltd Adherent cells from placenta tissue and use thereof in therapy
NZ593235A (en) * 2008-11-03 2013-02-22 Crucell Holland Bv Method for the production of recombinant adenovirus serotype 35
KR101548790B1 (ko) 2009-07-16 2015-08-31 크루셀 홀란드 비.브이. 백신 제조를 위한 고역가 폴리오바이러스의 제조
BR112012008516B1 (pt) 2009-10-15 2021-10-19 Janssen Vaccines & Prevention B.V. Método para purificar partículas de adenovírus a partir de uma suspensão de célula
EA028875B1 (ru) 2009-10-15 2018-01-31 Янссен Вэксинс Энд Превеншн Б.В. Способ очистки аденовирусных частиц из клеточной суспензии
PT2501822T (pt) 2009-11-17 2017-10-18 Squibb & Sons Llc Métodos para produção de proteínas melhorada
EA023816B1 (ru) * 2010-02-15 2016-07-29 Круселл Холланд Б.В. СПОСОБ ПРОДУЦИРОВАНИЯ ВИРУСНЫХ ЧАСТИЦ Ad26
US20120315696A1 (en) * 2010-02-15 2012-12-13 Alfred Luitjens METHOD FOR THE PRODUCTION OF Ad26 ADENOVIRAL VECTORS
WO2011144561A1 (en) 2010-05-18 2011-11-24 Crucell Holland B.V. Methods for welding ethylene vinyl acetate (eva) tubing, tubing obtained thereby and use of such tubing for sterile transfer of content into a bioreactor
JP6282467B2 (ja) * 2010-10-05 2018-02-21 ノヴォ・ノルディスク・ヘルス・ケア・アーゲー タンパク質を生産するための方法
EP3626737B1 (en) * 2011-05-13 2023-11-29 Octapharma AG A method of increasing the productivity of eucaryotic cells in the production of recombinant fviii
KR20230114317A (ko) * 2011-07-01 2023-08-01 암젠 인크 포유동물 세포 배양
US10485869B2 (en) 2011-10-18 2019-11-26 Coherus Biosciences, Inc. Etanercept formulations stabilized with meglumine
KR20140079491A (ko) 2011-10-18 2014-06-26 코히러스 바이오사이언시즈, 인코포레이티드 당과 폴리올과의 배합물로 안정화된 에타너셉트 제형
GB201211256D0 (en) * 2012-06-25 2012-08-08 Glaxosmithkline Biolog Sa Fermentation process
PE20150762A1 (es) 2012-07-09 2015-06-20 Coherus Biosciences Inc Formulaciones de etanercept que muestran una marcada reduccion de la cantidad de particulas subvisibles
SG11201501460RA (en) 2012-09-11 2015-04-29 Coherus Biosciences Inc Correctly folded etanercept in high purity and excellent yield
JP6321661B2 (ja) 2012-09-27 2018-05-09 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ タンジェンシャルフロー灌流システム及び方法
TWI637057B (zh) 2012-11-09 2018-10-01 拜爾沙納有限公司 具交替生物反應器用途之不連續進料批次製程
WO2014146933A1 (en) 2013-03-19 2014-09-25 Cmc Biologics A/S A method for producing a product (e.g. polypeptide) in a continuous cell culture fermentation process.
MX362922B (es) * 2013-03-26 2019-02-25 Coherus Biosciences Inc Método de producción de proteínas.
US9790465B2 (en) 2013-04-30 2017-10-17 Corning Incorporated Spheroid cell culture well article and methods thereof
CA2924332C (en) * 2013-09-16 2023-03-14 Genzyme Corporation Method of culturing cells using an open circuit filtration system with tangential flow filtration
AU2014326706A1 (en) 2013-09-30 2016-03-10 Janssen Vaccines & Prevention B.V. Method for the clarification of high density crude cell culture harvest
WO2015095809A1 (en) * 2013-12-20 2015-06-25 Biogen Idec Ma Inc. Use of perfusion seed cultures to improve biopharmaceutical fed-batch production capacity and product quality
CN113774084B (zh) * 2014-01-29 2024-04-16 美国安进公司 过表达n-糖基化途径调节基因以调节重组蛋白的糖基化
JP6385559B2 (ja) 2014-07-24 2018-09-05 ヤンセン ファッシンズ アンド プリベンション ベーフェーJanssen Vaccines & Prevention B.V. 細胞培養体からのポリオウイルスの精製プロセス
JP6530171B2 (ja) * 2014-09-09 2019-06-12 旭化成メディカル株式会社 培養産生物の回収方法
CN107426990A (zh) * 2014-09-18 2017-12-01 建新公司 超高密度细胞建库方法
JP2017532971A (ja) 2014-10-29 2017-11-09 コーニング インコーポレイテッド 細胞培養集合体を生成するためのマイクロウェル設計および製造
JP6930914B2 (ja) 2014-10-29 2021-09-01 コーニング インコーポレイテッド 灌流バイオリアクタ・プラットフォーム
JP6731916B2 (ja) 2014-10-29 2020-07-29 コーニング インコーポレイテッド 細胞培養インサート
WO2016073858A1 (en) 2014-11-07 2016-05-12 Genesis Technologies, Llc Linear reciprocating actuator
US11306341B2 (en) 2014-12-22 2022-04-19 Genzyme Corporation Methods of culturing a mammalian cell
CA3018264A1 (en) 2016-04-12 2017-10-19 Artemis Biosystems, Inc. Perfusion filtration systems
HRP20251357T1 (hr) 2016-04-14 2025-12-19 Ferring Ventures Ltd Bioreaktor s nepomičnim slojem i sustavom pumpe/cijevi konstantnog protoka
SG11201808698TA (en) * 2016-04-14 2018-11-29 Trizell Ltd Fixed-bed bioreactor with constant-flow pump/ tubing system
US12157877B2 (en) 2016-04-15 2024-12-03 Boehringer Ingelheim International Gmbh Cell retention device and method
JPWO2018003476A1 (ja) 2016-06-30 2019-01-17 富士フイルム株式会社 細胞懸濁液の膜分離方法及び細胞培養装置
PL3652292T3 (pl) 2017-07-14 2025-09-01 Corning Incorporated Naczynie do hodowli komórkowej 3d i sposoby hodowli komórkowej 3d
JP7197557B2 (ja) 2017-07-14 2022-12-27 コーニング インコーポレイテッド 多孔性支持体を有する細胞培養槽
US11857970B2 (en) 2017-07-14 2024-01-02 Corning Incorporated Cell culture vessel
CN111051494B (zh) 2017-07-14 2024-03-29 康宁股份有限公司 用于手动或自动培养基交换的3d细胞培养容器
EP3663390A4 (en) 2017-09-06 2020-08-05 FUJIFILM Corporation PROCESS FOR MANUFACTURING A PRODUCT
KR20200047702A (ko) * 2017-09-15 2020-05-07 브리스톨-마이어스 스큅 컴퍼니 관심 폴리펩티드 대규모 생산 동안의 온라인 바이오매스 정전용량 모니터링
WO2019071076A1 (en) 2017-10-06 2019-04-11 Lonza Ltd. AUTOMATED CONTROL OF CELL CULTURE BY SPECTROSCOPY RAMAN
EP3697887B1 (en) 2017-10-16 2022-04-06 Regeneron Pharmaceuticals, Inc. Perfusion methods
AU2018383679B2 (en) * 2017-12-11 2025-10-09 Amgen Inc. Continuous manufacturing process for bispecific antibody products
US10538727B2 (en) * 2018-03-08 2020-01-21 Repligen Corporation Tangential flow depth filtration systems and methods of filtration using same
CN110241012B (zh) * 2018-03-09 2022-11-01 嘉和生物药业有限公司 一种生物大分子上游分阶段截留的生产方法、生产模块及在生产中的应用
CN111868249B (zh) * 2018-03-19 2024-06-18 富士胶片株式会社 生产物的制造方法
KR102819571B1 (ko) 2018-05-25 2025-06-12 리플리겐 코포레이션 접선 유동 여과 시스템 및 방법
CN108504562A (zh) * 2018-06-21 2018-09-07 江苏澳创生物科技有限公司 一种发酵生产l-苏氨酸的系统及其应用
CN111065725B (zh) 2018-07-13 2024-03-29 康宁股份有限公司 包括具有互联的壁的微板的流体装置
EP3649229B1 (en) 2018-07-13 2021-08-25 Corning Incorporated Cell culture vessels with stabilizer devices
US11912968B2 (en) 2018-07-13 2024-02-27 Corning Incorporated Microcavity dishes with sidewall including liquid medium delivery surface
WO2020165873A1 (en) * 2019-02-15 2020-08-20 Oncosimis Biotech Private Limited Method and system for enhanced continuous production, secretion and separation of recombinant polypeptides from bacteria
EP3962536A1 (en) * 2019-04-29 2022-03-09 Voyager Therapeutics, Inc. Systems and methods for producing baculoviral infected insect cells (biics) in bioreactors
WO2020252442A1 (en) * 2019-06-13 2020-12-17 Amgen Inc. Automated biomass-based perfusion control in the manufacturing of biologics
JP2023503849A (ja) * 2019-11-15 2023-02-01 ロンザ リミテッド 接種物を生産するためのプロセスおよびシステム
US20230287322A1 (en) 2020-07-28 2023-09-14 Seagen Inc. Methods and systems for producing polypeptides
KR20230170728A (ko) * 2021-04-14 2023-12-19 젠자임 코포레이션 포유류 세포의 관류 배양 방법
CA3266599A1 (en) 2022-09-07 2024-11-07 Quantitative Biosciences, Inc. UNIQUE VARIABLE DOMAIN ANTIBODIES SPECIFIC TO FENTANYL AND USE IN A CONTINUOUS AGGLUTINATION TEST
CN116179464A (zh) * 2022-12-06 2023-05-30 无锡药明生物技术股份有限公司 提高培养液细胞密度和高密度细胞培养的方法
WO2024192116A1 (en) * 2023-03-14 2024-09-19 Repligen Corporation High viable cell density transfection
AU2024235963A1 (en) * 2023-03-14 2025-08-07 Sonnet BioTherapeutics, Inc. Methods of making recombinant il-12 albumin binding domain fusion proteins
WO2024226439A1 (en) 2023-04-24 2024-10-31 Quantitative Biosciences, Inc. Engineered biosensor strains of e. coli for continuous aerobic detection of analytes
WO2024248119A1 (ja) * 2023-05-31 2024-12-05 富士フイルム株式会社 生産物の生産方法
WO2025128343A1 (en) 2023-12-11 2025-06-19 Just-Evotec Biologics, Inc. Protein expression using trans-splicing and split selectable markers

Family Cites Families (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US54544A (en) * 1866-05-08 Improvement in low-water detecters
JPS61199788A (ja) 1985-02-28 1986-09-04 Mitsubishi Rayon Co Ltd 醗酵生成物の分離濃縮方法及び連続醗酵方法
US5286646A (en) 1985-11-25 1994-02-15 Boehringer Mannheim Gmbh Method for mammalian cell culture
GB8617646D0 (en) 1986-07-18 1986-08-28 Manchester Inst Science Tech Recovery of biological material
US4806484A (en) 1987-08-07 1989-02-21 Igb Products, Ltd. Perfusion airlift bioreactor
JPH0797982B2 (ja) 1987-09-24 1995-10-25 東洋紡績株式会社 細胞培養装置
US4921792A (en) * 1987-11-27 1990-05-01 Miles Inc. Continuous cell dispersion, cultivation and substance recovery process
JPH01243985A (ja) 1988-03-25 1989-09-28 P C C Technol:Kk 植物器官の培養法及びその培養槽
US5595909A (en) * 1988-05-23 1997-01-21 Regents Of The University Of Minnesota Filter device
DE68917642T2 (de) 1988-05-25 1995-02-09 Teijin Ltd Verfahren zur kontinuierlichen Züchtung von haftenden tierischen Zellen.
JPH02113896A (ja) * 1988-10-24 1990-04-26 Snow Brand Milk Prod Co Ltd ヒト抗リシンモノクローナル抗体およびそれを分泌するハイブリドーマ
US5019512A (en) * 1989-03-17 1991-05-28 Baxter International Inc. Spin filter for removing substantially cell-free culture medium from suspension cell culture system
JPH0541984A (ja) * 1990-09-07 1993-02-23 Dow Chem Co:The 攪拌容器における中空繊維においての細胞の増殖
US5256294A (en) 1990-09-17 1993-10-26 Genentech, Inc. Tangential flow filtration process and apparatus
GB9118664D0 (en) 1991-08-30 1991-10-16 Celltech Ltd Cell culture
JPH05153973A (ja) 1991-12-03 1993-06-22 Hitachi Ltd 細胞培養方法及び装置
US6204000B1 (en) 1995-04-28 2001-03-20 The United States Of America As Represented By The Department Of Health And Human Services Diagnostic methods and gene therapy using reagents derived from the human metastasis suppressor gene KAI1
JP2752918B2 (ja) * 1995-05-16 1998-05-18 ユーエイチティー株式会社 動物細胞のマイクロキャリア培養法
US6001585A (en) 1997-11-14 1999-12-14 Cellex Biosciences, Inc. Micro hollow fiber bioreactor
JP2997771B2 (ja) * 1998-06-29 2000-01-11 金沢大学長 細胞の懸濁培養方法
US8026096B1 (en) 1998-10-08 2011-09-27 Protein Sciences Corporation In vivo active erythropoietin produced in insect cells
US6103526A (en) 1998-10-08 2000-08-15 Protein Sciences Corporation Spodoptera frugiperda single cell suspension cell line in serum-free media, methods of producing and using
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
CA2252972C (en) 1998-11-26 2012-09-18 National Research Council Of Canada Serum-free production of recombinant proteins and adenoviral vectors
EP1147180B1 (en) * 1999-01-26 2009-01-07 Schering-Plough Ltd. Propagation of bovine coronavirus in chinese hamster ovary cells
WO2000063403A2 (en) * 1999-04-15 2000-10-26 Crucell Holland B.V. Recombinant protein production in a human cell using sequences encoding adenovirus e1 protein
US6855544B1 (en) * 1999-04-15 2005-02-15 Crucell Holland B.V. Recombinant protein production in a human cell
EP1103610A1 (en) * 1999-11-26 2001-05-30 Introgene B.V. Production of vaccines from immortalised mammalian cell lines
JP2001149099A (ja) 1999-11-26 2001-06-05 Olympus Optical Co Ltd 染色体異常解析のための有核細胞の標本作製方法
US6544424B1 (en) 1999-12-03 2003-04-08 Refined Technology Company Fluid filtration system
US6168941B1 (en) * 2000-04-07 2001-01-02 Genvec, Inc. Method of producing adenoviral vector stocks
JP4138209B2 (ja) 2000-06-29 2008-08-27 エーザイ・アール・アンド・ディー・マネジメント株式会社 プラバスタチン含有組成物
JP2002113896A (ja) 2000-10-05 2002-04-16 Seiko Epson Corp 画像形成装置
KR100882560B1 (ko) * 2001-04-17 2009-02-12 세이렌가부시끼가이샤 배지 첨가제 및 동물 세포 배양용 배지
DE10120835C2 (de) 2001-04-27 2003-04-10 Sifin Inst Fuer Immunpraeparat Verfahren zur Herstellung von monoklonalen Antikörpern
CA2351156A1 (en) 2001-07-04 2003-01-04 Peter W. Zandstra A bioprocess for the generation of pluripotent cell derived cells and tissues
KR100909316B1 (ko) * 2001-08-31 2009-07-24 바이엘 헬스케어 아게 고세포밀도 발효를 수행하기 위한 유닛 및 방법
CN1551778A (zh) 2001-09-05 2004-12-01 ɽ����ʽ���� 用于糖尿病神经病变的药物组合物
WO2003025158A1 (en) 2001-09-14 2003-03-27 Medcell Biologics, Inc. Oxygen enriched bioreactor and method of culturing cells
JP2003219873A (ja) 2002-01-24 2003-08-05 Japan Science & Technology Corp サケ科キングサーモン由来の不死化細胞
US20030186335A1 (en) 2002-03-28 2003-10-02 National Institute Of Advanced Industrial Science And Technology Monoclonal antibodies, hybridomas, cell isolation method, isolated cells and immunological diagnostic method
DE10237082B4 (de) * 2002-08-09 2014-12-31 Sartorius Stedim Biotech Gmbh Verfahren und Vorrichtung zur biotechnologischen Herstellung von Wertstoffen
CA2520891C (en) 2003-05-09 2014-07-08 Crucell Holland B.V. Cultures of e1-immortalized cells and processes for culturing the same to increase product yields therefrom
JP2005041984A (ja) 2003-07-22 2005-02-17 Shin Kobe Electric Mach Co Ltd 水架橋ポリオレフィン製シートの製造法
PL1720972T3 (pl) 2004-03-05 2014-06-30 Dpx Holdings Bv Proces hodowli komórek poprzez ciągłą perfuzję i zmienny przepływ styczny
AU2007272054C1 (en) 2006-07-14 2014-08-28 Dpx Holdings B.V. Improved process for the culturing of cells
JP2008308560A (ja) 2007-06-13 2008-12-25 Tohcello Co Ltd 表面防汚性複合フィルムの製造方法

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103305417A (zh) * 2012-03-07 2013-09-18 无锡药明康德生物技术有限公司 进行蛋白生产的高产量反应器及其生产方法和应用
CN112410279A (zh) * 2013-03-15 2021-02-26 建新公司 高密度细胞存储方法
CN103525756A (zh) * 2013-10-23 2014-01-22 南京农业大学 一种原代鸡肝细胞的分离培养方法
CN103525756B (zh) * 2013-10-23 2015-04-08 南京农业大学 一种原代鸡肝细胞的分离培养方法
CN106414490A (zh) * 2014-02-17 2017-02-15 爱德技术生物科学有限公司 使用基于膜的细胞截留系统增强重组蛋白的表达
CN106687575A (zh) * 2014-06-04 2017-05-17 美国安进公司 用于收获哺乳动物细胞培养物的方法
CN106687575B (zh) * 2014-06-04 2021-04-30 美国安进公司 用于收获哺乳动物细胞培养物的方法
CN107532132A (zh) * 2015-02-09 2018-01-02 优尼沃赛尔斯公司 用于生产细胞和/或细胞产品的系统、装置和方法
CN109714962A (zh) * 2016-07-11 2019-05-03 耶路撒冷希伯来大学的益生研究开发有限公司 用于在体外培养细胞的系统和方法
US12478571B2 (en) 2018-12-17 2025-11-25 Pontis Biologics, Inc. Enzyme based compositions and methods for removing dental calculi
WO2021008571A1 (zh) * 2019-07-16 2021-01-21 信达生物制药(苏州)有限公司 高密度连续接种的细胞培养方法及其应用
CN114127260A (zh) * 2019-07-16 2022-03-01 信达生物制药(苏州)有限公司 高密度连续接种的细胞培养方法及其应用

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