KR101050585B1 - 연속식 살포 및 교호 접선 흐름에 의한 세포 배양 방법 - Google Patents

연속식 살포 및 교호 접선 흐름에 의한 세포 배양 방법 Download PDF

Info

Publication number
KR101050585B1
KR101050585B1 KR1020067018075A KR20067018075A KR101050585B1 KR 101050585 B1 KR101050585 B1 KR 101050585B1 KR 1020067018075 A KR1020067018075 A KR 1020067018075A KR 20067018075 A KR20067018075 A KR 20067018075A KR 101050585 B1 KR101050585 B1 KR 101050585B1
Authority
KR
South Korea
Prior art keywords
cells
cell
cell culture
culture
density
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
KR1020067018075A
Other languages
English (en)
Korean (ko)
Other versions
KR20070022221A (ko
Inventor
존 크로레이
마이크 우벤
마틴 조스 매뉴엘 코코
Original Assignee
디에스엠 아이피 어셋츠 비.브이.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34961830&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR101050585(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 디에스엠 아이피 어셋츠 비.브이. filed Critical 디에스엠 아이피 어셋츠 비.브이.
Publication of KR20070022221A publication Critical patent/KR20070022221A/ko
Application granted granted Critical
Publication of KR101050585B1 publication Critical patent/KR101050585B1/ko
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/06Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/10Hollow fibers or tubes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10Perfusion
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0618Cells of the nervous system
    • C12N5/0621Eye cells, e.g. cornea, iris pigmented cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14Specific host cells or culture conditions, e.g. components, pH or temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2521/00Culture process characterised by the use of hydrostatic pressure, flow or shear forces

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Ophthalmology & Optometry (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Water Supply & Treatment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020067018075A 2004-03-05 2005-03-04 연속식 살포 및 교호 접선 흐름에 의한 세포 배양 방법 Ceased KR101050585B1 (ko)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
EP04075703.1 2004-03-05
EP04075702.3 2004-03-05
EP04075703 2004-03-05
EP04075702 2004-03-05
EP04077656 2004-09-27
EP04077657.7 2004-09-27
EP04077656.9 2004-09-27
EP04077657 2004-09-27
PCT/EP2005/002374 WO2005095578A1 (en) 2004-03-05 2005-03-04 Process for cell culturing by continuous perfusion and alternating tangential flow

Publications (2)

Publication Number Publication Date
KR20070022221A KR20070022221A (ko) 2007-02-26
KR101050585B1 true KR101050585B1 (ko) 2011-07-19

Family

ID=34961830

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020067018075A Ceased KR101050585B1 (ko) 2004-03-05 2005-03-04 연속식 살포 및 교호 접선 흐름에 의한 세포 배양 방법

Country Status (14)

Country Link
US (3) US8206981B2 (enExample)
EP (3) EP2540815A1 (enExample)
JP (6) JP2007525984A (enExample)
KR (1) KR101050585B1 (enExample)
CN (3) CN1930281A (enExample)
AU (1) AU2005229359C1 (enExample)
CA (1) CA2557046A1 (enExample)
DK (1) DK1720972T3 (enExample)
ES (1) ES2456015T3 (enExample)
IL (2) IL177724A (enExample)
PL (1) PL1720972T3 (enExample)
PT (1) PT1720972E (enExample)
SI (1) SI1720972T1 (enExample)
WO (1) WO2005095578A1 (enExample)

Families Citing this family (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2456015T3 (es) 2004-03-05 2014-04-21 Dsm Ip Assets B.V. Procedimiento para cultivar células mediante perfusión continua y flujo tangencial alternante
WO2007097260A1 (ja) 2006-02-24 2007-08-30 Toray Industries, Inc. 化学品の製造方法、および、連続発酵装置
EP1870450A1 (en) * 2006-06-21 2007-12-26 DSMIP Assets B.V. Process for the culturing of E1-immortalized HER cells
AU2011211462C1 (en) * 2006-07-14 2015-01-22 Dpx Holdings B.V. Improved process for the culturing of cells
CN102876628A (zh) * 2006-07-14 2013-01-16 帝斯曼知识产权资产管理有限公司 改进的细胞培养方法
EP2014760A1 (en) 2007-06-13 2009-01-14 CMC Biopharmaceuticals A/S A method for producing a biopolymer (e.g. polypeptide) in a continuous fermentation process
AU2008345231B2 (en) * 2007-12-27 2014-09-11 Takeda Pharmaceutical Company Limited Cell culture processes
WO2010003759A2 (en) * 2008-06-17 2010-01-14 Dsm Ip Assets B.V. Cell culturing method
EP3115451A1 (en) 2008-09-02 2017-01-11 Pluristem Ltd. Adherent cells from placenta tissue and use thereof in therapy
DK2350268T3 (en) * 2008-11-03 2015-03-23 Crucell Holland Bv PROCEDURE FOR PRODUCING ADENOVIRUS VECTORS
KR101548790B1 (ko) 2009-07-16 2015-08-31 크루셀 홀란드 비.브이. 백신 제조를 위한 고역가 폴리오바이러스의 제조
WO2011045381A1 (en) 2009-10-15 2011-04-21 Crucell Holland B.V. Process for adenovirus purification from high cell density cultures
AU2010305765B2 (en) 2009-10-15 2015-07-02 Crucell Holland B.V. Method for the purification of adenovirus particles
NO2501822T3 (enExample) 2009-11-17 2018-01-13
US20120315696A1 (en) * 2010-02-15 2012-12-13 Alfred Luitjens METHOD FOR THE PRODUCTION OF Ad26 ADENOVIRAL VECTORS
JP5250155B2 (ja) * 2010-02-15 2013-07-31 クルセル ホランド ベー ヴェー Ad26アデノウイルスベクターの製造方法
WO2011144561A1 (en) 2010-05-18 2011-11-24 Crucell Holland B.V. Methods for welding ethylene vinyl acetate (eva) tubing, tubing obtained thereby and use of such tubing for sterile transfer of content into a bioreactor
CN107190034A (zh) * 2010-10-05 2017-09-22 诺沃—诺迪斯克保健股份有限公司 生产蛋白质的方法
US9115381B2 (en) * 2011-05-13 2015-08-25 Octapharma Ag Method of increasing the productivity of eucaryotic cells in the production of recombinant FVIII
KR20230114317A (ko) * 2011-07-01 2023-08-01 암젠 인크 포유동물 세포 배양
AR088381A1 (es) 2011-10-18 2014-05-28 Coherus Biosciences Inc Formulaciones de etanercept estabilizadas con meglumina
US10485869B2 (en) 2011-10-18 2019-11-26 Coherus Biosciences, Inc. Etanercept formulations stabilized with meglumine
CN103305417A (zh) * 2012-03-07 2013-09-18 无锡药明康德生物技术有限公司 进行蛋白生产的高产量反应器及其生产方法和应用
GB201211256D0 (en) * 2012-06-25 2012-08-08 Glaxosmithkline Biolog Sa Fermentation process
CA2878508A1 (en) 2012-07-09 2014-01-16 Coherus Biosciences, Inc. Etanercept formulations exhibiting marked reduction in sub-visible particles
SG11201501460RA (en) 2012-09-11 2015-04-29 Coherus Biosciences Inc Correctly folded etanercept in high purity and excellent yield
WO2014051503A1 (en) 2012-09-27 2014-04-03 Ge Healthcare Bio-Sciences Ab Tangential flow perfusion system
TWI637057B (zh) 2012-11-09 2018-10-01 拜爾沙納有限公司 具交替生物反應器用途之不連續進料批次製程
KR20210095233A (ko) * 2013-03-15 2021-07-30 젠자임 코포레이션 고-밀도 세포 뱅킹 방법
EP2976418B1 (en) 2013-03-19 2020-09-30 CMC Biologics A/S A method for producing a product (e.g. polypeptide) in a continuous cell culture fermentation process.
EP3391942A1 (en) 2013-03-26 2018-10-24 Coherus Biosciences, Inc. Protein production method
US9790465B2 (en) 2013-04-30 2017-10-17 Corning Incorporated Spheroid cell culture well article and methods thereof
SG10201803601SA (en) * 2013-09-16 2018-06-28 Genzyme Corp Methods and systems for processing a cell culture
BR112016006063A2 (pt) 2013-09-30 2017-08-01 Crucell Holland Bv método para clarificação de uma colheita de cultura celular em bruto
CN103525756B (zh) * 2013-10-23 2015-04-08 南京农业大学 一种原代鸡肝细胞的分离培养方法
EP3083933B1 (en) * 2013-12-20 2026-01-21 Biogen MA Inc. Use of perfusion seed cultures to improve biopharmaceutical fed-batch production capacity and product quality
IL282517B (en) * 2014-01-29 2022-07-01 Amgen Inc Overexpression of n-glycosylation pathway regulators to modulate glycosylation of recombinant proteins
EP3107934A4 (en) * 2014-02-17 2017-10-18 Advantech Bioscience Farmacêutica Ltda Enhancement of recombinant protein expression using a membrane-based cell retention system
PT3926051T (pt) * 2014-06-04 2024-06-20 Amgen Inc Métodos de colheita de culturas de células de mamíferos
CN106574252B (zh) 2014-07-24 2021-06-01 扬森疫苗与预防公司 从细胞培养物中纯化脊髓灰质炎病毒的方法
JP6530171B2 (ja) * 2014-09-09 2019-06-12 旭化成メディカル株式会社 培養産生物の回収方法
CN107426990A (zh) * 2014-09-18 2017-12-01 建新公司 超高密度细胞建库方法
KR102460969B1 (ko) 2014-10-29 2022-10-31 코닝 인코포레이티드 세포 배양 인서트
CN107109339B (zh) 2014-10-29 2021-07-02 康宁股份有限公司 灌注生物反应器平台
WO2016069930A1 (en) 2014-10-29 2016-05-06 Corning Incorporated Microwell design and fabrication for generation of cell culture aggregates
US10092888B2 (en) 2014-11-07 2018-10-09 Genesis Technologies, Llc Linear reciprocating actuator
WO2016106192A2 (en) 2014-12-22 2016-06-30 Genzyme Corporation Methods of culturing a mammalian cell
WO2016128361A1 (en) * 2015-02-09 2016-08-18 Univercells Nv System, apparatus and method for the production of cells and/or cell products
US10358626B2 (en) 2016-04-12 2019-07-23 Artemis Biosystems, Inc. Perfusion filtration systems
EP3604547A1 (en) * 2016-04-14 2020-02-05 Trizell Ltd. Fixed-bed bioreactor with constant-flow pump/tubing system
FI3722435T3 (fi) 2016-04-14 2025-12-05 Ferring Ventures Ltd Kiinteäpetinen bioreaktori vakiovirtauspumppu/-putkijärjestelmällä
KR102414290B1 (ko) 2016-04-15 2022-06-29 베링거 인겔하임 인터내셔날 게엠베하 세포 보유 장치 및 방법
JPWO2018003476A1 (ja) * 2016-06-30 2019-01-17 富士フイルム株式会社 細胞懸濁液の膜分離方法及び細胞培養装置
WO2018011805A2 (en) * 2016-07-11 2018-01-18 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Systems and methods for growing cells in vitro
US11857970B2 (en) 2017-07-14 2024-01-02 Corning Incorporated Cell culture vessel
EP3652291B1 (en) 2017-07-14 2021-12-29 Corning Incorporated Cell culture vessel
JP7195302B2 (ja) 2017-07-14 2022-12-23 コーニング インコーポレイテッド 3d培養のための細胞培養容器及び3d細胞の培養方法
CN111051494B (zh) 2017-07-14 2024-03-29 康宁股份有限公司 用于手动或自动培养基交换的3d细胞培养容器
CN111051496B (zh) 2017-09-06 2024-03-08 富士胶片株式会社 产物的制造方法
EP3681989A1 (en) * 2017-09-15 2020-07-22 Bristol-Myers Squibb Company Online biomass capacitance monitoring during large scale production of polypeptides of interest
KR20250151558A (ko) 2017-10-06 2025-10-21 론자 리미티드 라만 분광법을 사용하는 세포 배양의 자동 제어
CN118580928A (zh) 2017-10-16 2024-09-03 里珍纳龙药品有限公司 灌注生物反应器及相关使用方法
TWI827570B (zh) * 2017-12-11 2024-01-01 美商安進公司 雙特異性抗體產品之連續製造製程
CA3088023C (en) * 2018-03-08 2023-03-21 Repligen Corporation Tangential flow depth filtration systems and methods of filtration using same
CN110241012B (zh) * 2018-03-09 2022-11-01 嘉和生物药业有限公司 一种生物大分子上游分阶段截留的生产方法、生产模块及在生产中的应用
EP3770266A4 (en) * 2018-03-19 2021-06-09 FUJIFILM Corporation PRODUCT MANUFACTURING PROCESS
CN112165983A (zh) 2018-05-25 2021-01-01 瑞普利金公司 切向流过滤系统和方法
CN108504562A (zh) * 2018-06-21 2018-09-07 江苏澳创生物科技有限公司 一种发酵生产l-苏氨酸的系统及其应用
CN111065725B (zh) 2018-07-13 2024-03-29 康宁股份有限公司 包括具有互联的壁的微板的流体装置
EP3649229B1 (en) 2018-07-13 2021-08-25 Corning Incorporated Cell culture vessels with stabilizer devices
WO2020013847A1 (en) 2018-07-13 2020-01-16 Corning Incorporated Microcavity dishes with sidewall including liquid medium delivery surface
US12478571B2 (en) 2018-12-17 2025-11-25 Pontis Biologics, Inc. Enzyme based compositions and methods for removing dental calculi
WO2020165873A1 (en) * 2019-02-15 2020-08-20 Oncosimis Biotech Private Limited Method and system for enhanced continuous production, secretion and separation of recombinant polypeptides from bacteria
WO2020223274A1 (en) * 2019-04-29 2020-11-05 Voyager Therapeutics, Inc. SYSTEMS AND METHODS FOR PRODUCING BACULOVIRAL INFECTED INSECT CELLS (BIICs) IN BIOREACTORS
CA3137494A1 (en) * 2019-06-13 2020-12-17 Amgen Inc. Automated biomass-based perfusion control in the manufacturing of biologics
JP7680418B2 (ja) * 2019-07-16 2025-05-20 イノベント バイオロジクス(スーチョウ)カンパニー,リミティド 高密度連続接種の細胞培養方法及びその応用
EP4058547A1 (en) * 2019-11-15 2022-09-21 Lonza Ltd. Process and system for producing an inoculum
CA3189533A1 (en) 2020-07-28 2022-02-03 Seagen Inc. Methods and systems for producing polypeptides
JP2024514327A (ja) * 2021-04-14 2024-04-01 ジェンザイム・コーポレーション 哺乳類細胞の灌流培養方法
EP4584299A2 (en) 2022-09-07 2025-07-16 Quantitative Biosciences, Inc. Fentanyl-specific single variable-domain antibodies and use in a continuous agglutination assay
CN116179464A (zh) * 2022-12-06 2023-05-30 无锡药明生物技术股份有限公司 提高培养液细胞密度和高密度细胞培养的方法
WO2024192171A1 (en) * 2023-03-14 2024-09-19 Sonnet BioTherapeutics, Inc. Methods of making recombinant il-12 albumin binding domain fusion proteins
WO2024192116A1 (en) * 2023-03-14 2024-09-19 Repligen Corporation High viable cell density transfection
WO2024226439A1 (en) 2023-04-24 2024-10-31 Quantitative Biosciences, Inc. Engineered biosensor strains of e. coli for continuous aerobic detection of analytes
WO2024248119A1 (ja) * 2023-05-31 2024-12-05 富士フイルム株式会社 生産物の生産方法
WO2025128343A1 (en) 2023-12-11 2025-06-19 Just-Evotec Biologics, Inc. Protein expression using trans-splicing and split selectable markers

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000004876A (ja) * 1998-06-29 2000-01-11 Univ Kanazawa 細胞の懸濁培養方法
WO2003020919A2 (en) * 2001-08-31 2003-03-13 Bayer Healthcare Ag A unit and a process for carrying out high cell density fermentation
US6544424B1 (en) * 1999-12-03 2003-04-08 Refined Technology Company Fluid filtration system

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US54544A (en) * 1866-05-08 Improvement in low-water detecters
JPS61199788A (ja) 1985-02-28 1986-09-04 Mitsubishi Rayon Co Ltd 醗酵生成物の分離濃縮方法及び連続醗酵方法
US5286646A (en) 1985-11-25 1994-02-15 Boehringer Mannheim Gmbh Method for mammalian cell culture
GB8617646D0 (en) 1986-07-18 1986-08-28 Manchester Inst Science Tech Recovery of biological material
US4806484A (en) 1987-08-07 1989-02-21 Igb Products, Ltd. Perfusion airlift bioreactor
JPH0797982B2 (ja) 1987-09-24 1995-10-25 東洋紡績株式会社 細胞培養装置
US4921792A (en) 1987-11-27 1990-05-01 Miles Inc. Continuous cell dispersion, cultivation and substance recovery process
JPH01243985A (ja) 1988-03-25 1989-09-28 P C C Technol:Kk 植物器官の培養法及びその培養槽
US5595909A (en) * 1988-05-23 1997-01-21 Regents Of The University Of Minnesota Filter device
EP0343635B1 (en) 1988-05-25 1994-08-24 Teijin Limited Process for continuously culturing adherent animal cells
JPH02113896A (ja) * 1988-10-24 1990-04-26 Snow Brand Milk Prod Co Ltd ヒト抗リシンモノクローナル抗体およびそれを分泌するハイブリドーマ
US5019512A (en) * 1989-03-17 1991-05-28 Baxter International Inc. Spin filter for removing substantially cell-free culture medium from suspension cell culture system
JPH0541984A (ja) * 1990-09-07 1993-02-23 Dow Chem Co:The 攪拌容器における中空繊維においての細胞の増殖
US5256294A (en) 1990-09-17 1993-10-26 Genentech, Inc. Tangential flow filtration process and apparatus
GB9118664D0 (en) 1991-08-30 1991-10-16 Celltech Ltd Cell culture
JPH05153973A (ja) 1991-12-03 1993-06-22 Hitachi Ltd 細胞培養方法及び装置
US6204000B1 (en) 1995-04-28 2001-03-20 The United States Of America As Represented By The Department Of Health And Human Services Diagnostic methods and gene therapy using reagents derived from the human metastasis suppressor gene KAI1
JP2752918B2 (ja) * 1995-05-16 1998-05-18 ユーエイチティー株式会社 動物細胞のマイクロキャリア培養法
US6001585A (en) 1997-11-14 1999-12-14 Cellex Biosciences, Inc. Micro hollow fiber bioreactor
US6103526A (en) 1998-10-08 2000-08-15 Protein Sciences Corporation Spodoptera frugiperda single cell suspension cell line in serum-free media, methods of producing and using
US8026096B1 (en) 1998-10-08 2011-09-27 Protein Sciences Corporation In vivo active erythropoietin produced in insect cells
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
CA2252972C (en) 1998-11-26 2012-09-18 National Research Council Of Canada Serum-free production of recombinant proteins and adenoviral vectors
SI1147180T1 (sl) 1999-01-26 2009-06-30 Schering Plough Ltd Razmnoževnje govejega korona virusa v ovarijskih celicah kitajskega hrčka
US6855544B1 (en) * 1999-04-15 2005-02-15 Crucell Holland B.V. Recombinant protein production in a human cell
NZ514951A (en) 1999-04-15 2004-02-27 Crucell Holland B Recombinant protein production in a cultured human cell
JP2001149099A (ja) 1999-11-26 2001-06-05 Olympus Optical Co Ltd 染色体異常解析のための有核細胞の標本作製方法
EP1103610A1 (en) 1999-11-26 2001-05-30 Introgene B.V. Production of vaccines from immortalised mammalian cell lines
US6168941B1 (en) * 2000-04-07 2001-01-02 Genvec, Inc. Method of producing adenoviral vector stocks
JP4138209B2 (ja) 2000-06-29 2008-08-27 エーザイ・アール・アンド・ディー・マネジメント株式会社 プラバスタチン含有組成物
JP2002113896A (ja) 2000-10-05 2002-04-16 Seiko Epson Corp 画像形成装置
KR100882560B1 (ko) * 2001-04-17 2009-02-12 세이렌가부시끼가이샤 배지 첨가제 및 동물 세포 배양용 배지
DE10120835C2 (de) 2001-04-27 2003-04-10 Sifin Inst Fuer Immunpraeparat Verfahren zur Herstellung von monoklonalen Antikörpern
CA2351156A1 (en) 2001-07-04 2003-01-04 Peter W. Zandstra A bioprocess for the generation of pluripotent cell derived cells and tissues
ES2249616T3 (es) 2001-09-05 2006-04-01 Yamasa Corporation Citidina-fosfocolina para el tratamiento de la neuropatia diabetica.
WO2003025158A1 (en) 2001-09-14 2003-03-27 Medcell Biologics, Inc. Oxygen enriched bioreactor and method of culturing cells
JP2003219873A (ja) 2002-01-24 2003-08-05 Japan Science & Technology Corp サケ科キングサーモン由来の不死化細胞
US20030186335A1 (en) 2002-03-28 2003-10-02 National Institute Of Advanced Industrial Science And Technology Monoclonal antibodies, hybridomas, cell isolation method, isolated cells and immunological diagnostic method
DE10237082B4 (de) * 2002-08-09 2014-12-31 Sartorius Stedim Biotech Gmbh Verfahren und Vorrichtung zur biotechnologischen Herstellung von Wertstoffen
EP1623023B1 (en) 2003-05-09 2008-11-12 Crucell Holland B.V. Cultures of e1-immortalized cells and processes for culturing the same to increase product yields therefrom
JP2005041984A (ja) 2003-07-22 2005-02-17 Shin Kobe Electric Mach Co Ltd 水架橋ポリオレフィン製シートの製造法
ES2456015T3 (es) 2004-03-05 2014-04-21 Dsm Ip Assets B.V. Procedimiento para cultivar células mediante perfusión continua y flujo tangencial alternante
CN102876628A (zh) 2006-07-14 2013-01-16 帝斯曼知识产权资产管理有限公司 改进的细胞培养方法
JP2008308560A (ja) 2007-06-13 2008-12-25 Tohcello Co Ltd 表面防汚性複合フィルムの製造方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000004876A (ja) * 1998-06-29 2000-01-11 Univ Kanazawa 細胞の懸濁培養方法
US6544424B1 (en) * 1999-12-03 2003-04-08 Refined Technology Company Fluid filtration system
WO2003020919A2 (en) * 2001-08-31 2003-03-13 Bayer Healthcare Ag A unit and a process for carrying out high cell density fermentation

Also Published As

Publication number Publication date
WO2005095578A1 (en) 2005-10-13
EP1720972A1 (en) 2006-11-15
AU2005229359C1 (en) 2011-07-28
JP5719947B2 (ja) 2015-05-20
DK1720972T3 (da) 2014-04-14
EP2308958A2 (en) 2011-04-13
KR20070022221A (ko) 2007-02-26
CA2557046A1 (en) 2005-10-13
JP2007525984A (ja) 2007-09-13
JP2016039817A (ja) 2016-03-24
ES2456015T3 (es) 2014-04-21
AU2005229359A1 (en) 2005-10-13
JP2011125348A (ja) 2011-06-30
US9260695B2 (en) 2016-02-16
EP1720972B1 (en) 2014-01-08
CN1930281A (zh) 2007-03-14
AU2005229359B2 (en) 2009-01-08
EP2308958A3 (en) 2011-08-10
JP2017225443A (ja) 2017-12-28
JP2014128272A (ja) 2014-07-10
CN104388324A (zh) 2015-03-04
JP2012090632A (ja) 2012-05-17
IL177724A (en) 2011-01-31
US20140087424A1 (en) 2014-03-27
IL177724A0 (en) 2006-12-31
US8206981B2 (en) 2012-06-26
PL1720972T3 (pl) 2014-06-30
EP2540815A1 (en) 2013-01-02
PT1720972E (pt) 2014-04-07
IL205606A (en) 2011-08-31
US20080131934A1 (en) 2008-06-05
SI1720972T1 (sl) 2014-06-30
US20130266985A1 (en) 2013-10-10
CN104593277A (zh) 2015-05-06

Similar Documents

Publication Publication Date Title
KR101050585B1 (ko) 연속식 살포 및 교호 접선 흐름에 의한 세포 배양 방법
US9670520B2 (en) Process for the culturing of cells
AU2013203461B2 (en) Process for cell culturing by continuous perfusion and alternating tangential flow
CN101490239A (zh) 改进的细胞培养方法
HK1179646A (en) Perfusion cell culture
HK1104189A (en) Process for cell culturing by continuous perfusion and alternating tangential flow

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20060905

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20100304

Comment text: Request for Examination of Application

A302 Request for accelerated examination
PA0302 Request for accelerated examination

Patent event date: 20101014

Patent event code: PA03022R01D

Comment text: Request for Accelerated Examination

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20101127

Patent event code: PE09021S01D

E90F Notification of reason for final refusal
PE0902 Notice of grounds for rejection

Comment text: Final Notice of Reason for Refusal

Patent event date: 20110407

Patent event code: PE09021S02D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20110620

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20110713

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20110713

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
J204 Request for invalidation trial [patent]
PJ0204 Invalidation trial for patent

Patent event date: 20111013

Comment text: Request for Trial

Patent event code: PJ02042R01D

Patent event date: 20110713

Comment text: Registration of Establishment

Patent event code: PJ02041E01I

Appeal kind category: Invalidation

Request date: 20111013

Decision date: 20130124

Appeal identifier: 2011100002531

J301 Trial decision

Free format text: TRIAL DECISION FOR INVALIDATION REQUESTED 20111013

Effective date: 20130124

PJ1301 Trial decision

Patent event code: PJ13011S05D

Patent event date: 20130125

Comment text: Trial Decision on Invalidation (Patent, Utility Model, Industrial Design)

Appeal kind category: Invalidation

Request date: 20111013

Decision date: 20130124

Appeal identifier: 2011100002531

J2X1 Appeal (before the patent court)

Free format text: INVALIDATION

PJ2001 Appeal

Patent event date: 20130125

Comment text: Trial Decision on Invalidation (Patent, Utility Model, Industrial Design)

Patent event code: PJ20011S05I

Appeal kind category: Invalidation

Decision date: 20140206

Appeal identifier: 2013200001740

Request date: 20130228

PJ2002 Appeal before the supreme court

Comment text: Trial Decision on Invalidation (Patent, Utility Model, Industrial Design)

Patent event date: 20130125

Patent event code: PJ20021S05I

Request date: 20140319

Appeal identifier: 2014300000584

Appeal kind category: Invalidation

Decision date: 20140710

J302 Written judgement (patent court)

Free format text: JUDGMENT (PATENT COURT) FOR INVALIDATION REQUESTED 20130228

Effective date: 20140206

PJ1302 Judgment (patent court)

Patent event date: 20140324

Comment text: Written Judgment (Patent Court)

Patent event code: PJ13021S01D

Request date: 20130228

Decision date: 20140206

Appeal identifier: 2013200001740

Appeal kind category: Invalidation

FPAY Annual fee payment

Payment date: 20140702

Year of fee payment: 4

PR1001 Payment of annual fee

Payment date: 20140702

Start annual number: 4

End annual number: 4

J221 Remand (intellectual property tribunal)

Free format text: REMAND (INTELLECTUAL PROPERTY TRIBUNAL) FOR INVALIDATION

J303 Written judgement (supreme court)

Free format text: JUDGMENT (SUPREME COURT) FOR INVALIDATION REQUESTED 20140319

Effective date: 20140710

PJ1303 Judgment (supreme court)

Comment text: Written Judgment (Supreme Court)

Patent event date: 20140725

Patent event code: PJ13031S01D

Decision date: 20140710

Appeal kind category: Invalidation

Request date: 20140319

Appeal identifier: 2014300000584

PJ2201 Remand (intellectual property tribunal)

Request date: 20140725

Appeal kind category: Invalidation

Appeal identifier: 2014130000108

Decision date: 20140819

J301 Trial decision

Free format text: TRIAL DECISION FOR INVALIDATION REQUESTED 20140725

Effective date: 20140819

PJ1301 Trial decision

Patent event code: PJ13011S09D

Patent event date: 20140819

Comment text: Trial Decision on Final Judgment on Revocation

Appeal kind category: Invalidation

Request date: 20140725

Decision date: 20140819

Appeal identifier: 2014130000108

PC2102 Extinguishment

Termination category: Others

Termination date: 20141117

PG1701 Publication of correction

Publication date: 20150320