KR101050585B1 - 연속식 살포 및 교호 접선 흐름에 의한 세포 배양 방법 - Google Patents
연속식 살포 및 교호 접선 흐름에 의한 세포 배양 방법 Download PDFInfo
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Abstract
Description
Claims (22)
- 세포 배양액 중 응집체를 형성하는 고유의 경향을 갖는 세포의 응집 정도를 감소시키는 방법으로서,세포 배양 배지 및 응집체를 형성하는 고유의 경향을 갖는 세포의 현탁액을 포함하는 세포 배양액이 연속식 살포(perfusion) 배양계에서 유지되고, 세포 배양 배지를 세포 배양액에 첨가하고, 세포 배양액보다 낮은 세포 밀도를 갖는 액체의 유출을 초래하는 중공사(hollow fiber)를 포함하는 필터 모듈상에서 세포 배양액을 순환시키고, 필터 모듈내의 흐름이 교호 접선 흐름이며, 목적하는 세포 밀도에 도달하는 동안 상기 배양이 계속되는, 방법.
- 삭제
- 삭제
- 제 1 항에 있어서,바이오매스가 세포 배양액으로부터 1회 이상 제거되고 추가의 세포 배양 배지를 세포 배양액에 첨가하는 방법.
- 제 4 항에 있어서,바이오매스 제거를, 세포가 정상 상태(steady state)에 도달하기 직전 또는 도달한 직후에 시작하는 방법.
- 제 4 항에 있어서,바이오매스의 체적이 1일 당 세포 배양액의 총 체적의 2 내지 40%로 제거되는 방법.
- 제 1 항에 있어서,하나의 펌프를 사용하여 중공사를 포함하는 섬유 모듈상에서 세포 배양액을 순환시키고 또다른 펌프를 사용하여 필터 분리 이전의 세포 배양액 보다 낮은 세포 밀도를 갖는 액체를 제거함으로써, 교호 접선 흐름을 수행하는 방법.
- 삭제
- 삭제
- 삭제
- 제 1 항에 있어서,응집체를 형성하는 고유의 경향을 갖는 세포가 포유동물 세포인 방법.
- 제 1 항에 있어서,응집체를 형성하는 고유의 경향을 갖는 세포가 PER.C6 세포인 방법.
- 제 1 항, 제 4 항 내지 제 7 항, 제 11 항 또는 제 12 항 중 어느 한 항에 있어서,세포가 생물학적 물질을 생산하는 방법.
- 제 13 항에 있어서,생물학적 물질이 치료 또는 진단 단백질인 방법.
- 제 13 항에 있어서,생물학적 물질이 세포 배양액을 순환시키는 단계 후의 하류 공정에서 추가로 정제되는 방법.
- 세포 배양 배지 및 응집체를 형성하는 고유의 경향을 갖는 동물 세포의 현탁액을 포함하는 살포 배양액으로서, 상기 동물 세포가 상기 살포 배양액 중 80 x 106세포/㎖ 이상의 밀도로 존재하고 5개 이상의 세포의 응집체가 세포 총량의 5% 이하로 포함된 살포 배양액.
- 제 16 항에 있어서,상기 동물 세포가 상기 살포 배양액 중 100 x 106세포/㎖ 이상의 밀도로 존재하는 살포 배양액.
- 제 16 항에 있어서,상기 동물 세포가 상기 살포 배양액 중 110 x 106세포/㎖ 이상의 밀도로 존재하는 살포 배양액.
- 제 16 항에 있어서,상기 동물 세포가 상기 살포 배양액 중 120 x 106세포/㎖ 이상의 밀도로 존재하는 살포 배양액.
- 제 16 항에 있어서,상기 동물 세포가 상기 살포 배양액 중 130 x 106세포/㎖ 이상의 밀도로 존재하는 살포 배양액.
- 제 16 항에 있어서,상기 동물 세포가 상기 살포 배양액 중 140 x 106세포/㎖ 이상의 밀도로 존재하는 살포 배양액.
- 제 16 항 또는 제 21 항에 있어서,상기 동물 세포가 PER.C6 세포인 살포 배양액.
Applications Claiming Priority (9)
Application Number | Priority Date | Filing Date | Title |
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EP04075702.3 | 2004-03-05 | ||
EP04075703 | 2004-03-05 | ||
EP04075702 | 2004-03-05 | ||
EP04075703.1 | 2004-03-05 | ||
EP04077657.7 | 2004-09-27 | ||
EP04077657 | 2004-09-27 | ||
EP04077656 | 2004-09-27 | ||
EP04077656.9 | 2004-09-27 | ||
PCT/EP2005/002374 WO2005095578A1 (en) | 2004-03-05 | 2005-03-04 | Process for cell culturing by continuous perfusion and alternating tangential flow |
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KR20070022221A KR20070022221A (ko) | 2007-02-26 |
KR101050585B1 true KR101050585B1 (ko) | 2011-07-19 |
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KR1020067018075A KR101050585B1 (ko) | 2004-03-05 | 2005-03-04 | 연속식 살포 및 교호 접선 흐름에 의한 세포 배양 방법 |
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US (3) | US8206981B2 (ko) |
EP (3) | EP2308958A3 (ko) |
JP (6) | JP2007525984A (ko) |
KR (1) | KR101050585B1 (ko) |
CN (3) | CN104593277A (ko) |
AU (1) | AU2005229359C1 (ko) |
CA (1) | CA2557046A1 (ko) |
DK (1) | DK1720972T3 (ko) |
ES (1) | ES2456015T3 (ko) |
IL (2) | IL177724A (ko) |
PL (1) | PL1720972T3 (ko) |
PT (1) | PT1720972E (ko) |
SI (1) | SI1720972T1 (ko) |
WO (1) | WO2005095578A1 (ko) |
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