CN105861443A - 体细胞重编程 - Google Patents

体细胞重编程 Download PDF

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Publication number
CN105861443A
CN105861443A CN201610213311.5A CN201610213311A CN105861443A CN 105861443 A CN105861443 A CN 105861443A CN 201610213311 A CN201610213311 A CN 201610213311A CN 105861443 A CN105861443 A CN 105861443A
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cells
cell
reprogramming
somatic
expression
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Chinese (zh)
Inventor
鲁道夫·耶尼施
雅各布·汉纳
马里厄斯·韦尼希
克里斯多佛·J·莱恩奈尔
亚历山大·迈斯纳
奥利弗·托比亚斯·布兰布林克
G·格朗特·韦尔斯泰德
鲁思·福尔曼
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Whitehead Institute for Biomedical Research
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Whitehead Institute for Biomedical Research
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Publication of CN105861443A publication Critical patent/CN105861443A/zh
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CN201610213311.5A 2007-04-07 2008-04-07 体细胞重编程 Pending CN105861443A (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US92212107P 2007-04-07 2007-04-07
US60/922,121 2007-04-07
US95934107P 2007-07-12 2007-07-12
US60/959,341 2007-07-12
US3606508P 2008-03-12 2008-03-12
US61/036,065 2008-03-12
CN2008800151822A CN101932705A (zh) 2007-04-07 2008-04-07 体细胞重编程

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CN201610213311.5A Pending CN105861443A (zh) 2007-04-07 2008-04-07 体细胞重编程

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US (5) US9382515B2 (enExample)
EP (3) EP2626416A3 (enExample)
JP (8) JP2010523117A (enExample)
CN (2) CN101932705A (enExample)
AU (1) AU2008236629B2 (enExample)
CA (2) CA2683056C (enExample)
MX (2) MX352541B (enExample)
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CN112201307A (zh) * 2020-09-23 2021-01-08 温州医科大学 一种转录本注释方法以及筛选长非编码rna和内源逆转录病毒来源长非编码rna的方法
CN113424794A (zh) * 2021-04-16 2021-09-24 安徽农业大学 一种优质抗病型地方鸡新品系选育方法及应用

Families Citing this family (157)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITRM20030376A1 (it) 2003-07-31 2005-02-01 Univ Roma Procedimento per l'isolamento e l'espansione di cellule staminali cardiache da biopsia.
US7682828B2 (en) 2003-11-26 2010-03-23 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
US11660317B2 (en) 2004-11-08 2023-05-30 The Johns Hopkins University Compositions comprising cardiosphere-derived cells for use in cell therapy
US8137907B2 (en) 2005-01-03 2012-03-20 Cold Spring Harbor Laboratory Orthotopic and genetically tractable non-human animal model for liver cancer and the uses thereof
CA2610265A1 (en) 2005-05-31 2007-05-10 Cold Spring Harbor Laboratory Methods for producing micrornas
JP4395193B2 (ja) 2005-08-01 2010-01-06 ニューポテンシャル，インコーポレイテッド 回復能を有する再プログラムされた細胞の作製
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
ZA200804673B (en) 2005-12-13 2009-11-25 Univ Kyoto Nuclear reprogramming factor
US20090227032A1 (en) 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
US8129187B2 (en) 2005-12-13 2012-03-06 Kyoto University Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2
CA2683056C (en) 2007-04-07 2020-03-24 Whitehead Institute For Biomedical Research Reprogramming of somatic cells
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
US9213999B2 (en) 2007-06-15 2015-12-15 Kyoto University Providing iPSCs to a customer
MX2010002242A (es) 2007-08-31 2010-06-01 Whitehead Biomedical Inst Estimulación de ruta wnt en reprogramacion de celulas somaticas.
WO2009067757A1 (en) * 2007-11-30 2009-06-04 Cytomatrix Pty Ltd Methods of inducing pluripotency involving oct4 protein
WO2009067756A1 (en) * 2007-11-30 2009-06-04 Cytomatrix Pty Ltd Methods of inducing pluripotency involving sox2 protein
KR101532442B1 (ko) * 2007-12-10 2015-06-29 고쿠리츠 다이가쿠 호진 교토 다이가쿠 효율적인 핵 초기화 방법
JP5626619B2 (ja) * 2008-12-08 2014-11-19 国立大学法人京都大学 効率的な核初期化方法
US9683232B2 (en) 2007-12-10 2017-06-20 Kyoto University Efficient method for nuclear reprogramming
EP2274424A4 (en) * 2008-04-07 2012-02-01 Nupotential Inc REPROGRAMMING A CELL BY INDUCING A PLURIPOTENT GENE USING AN HDAC MODULATOR
US20100279404A1 (en) 2008-05-02 2010-11-04 Shinya Yamanaka Method of nuclear reprogramming
CA3060170A1 (en) 2008-06-13 2009-12-17 Whitehead Institute For Biomedical Research Programming and reprogramming of cells
US9238797B2 (en) 2008-07-07 2016-01-19 Takara Bio Inc. Method for production of pluripotent stem cell
EP2333050A4 (en) * 2008-09-04 2013-07-03 Riken IPS CELLS FROM B-CELLS AND ITS APPLICATION
US20100062534A1 (en) * 2008-09-09 2010-03-11 The General Hospital Corporation Inducible lentiviral vectors for reprogramming somatic cells
WO2010056808A2 (en) * 2008-11-12 2010-05-20 The Regents Of The University Of California Compositions and methods for re-programming and re-differentiating cells
CN102245588B (zh) 2008-12-23 2016-05-04 塞格提斯有限公司 缩酮酰胺化合物、其制备方法及应用
EP2607476B1 (en) * 2009-02-27 2015-04-08 Kyoto University Novel nuclear reprogramming substance
KR101764437B1 (ko) * 2009-03-20 2017-08-02 메소블라스트, 아이엔씨. 재프로그램된 다분화능 세포의 생성 방법
CN101580816B (zh) * 2009-04-23 2012-02-29 中国科学院广州生物医药与健康研究院 诱导多能性干细胞快速高效产生的新型无血清培养基以及使用其的方法
WO2010124290A2 (en) * 2009-04-24 2010-10-28 Whitehead Institute For Biomedical Research Compositions and methods for deriving or culturing pluripotent cells
EP2451982B1 (en) 2009-07-09 2014-11-12 Whitehead Institute For Biomedical Research Compositions and methods for mammalian genetics and uses thereof
EP2468312A4 (en) 2009-08-19 2014-05-14 Univ Tohoku FOIL FOR CORNEAL TRANSPLANTS
EP2470664A4 (en) 2009-08-27 2013-01-16 Synaptic Res Llc NEW PROTEIN RELEASE SYSTEM FOR GENERATING INDUCED PLURIPOTENTAL STEM CELLS OR TISSUE-SPECIFIC CELLS
WO2011050470A1 (en) 2009-10-29 2011-05-05 Mcmaster University Generating induced pluripotent stem cells and progenitor cells from fibroblasts
WO2011079307A2 (en) * 2009-12-23 2011-06-30 The Salk Institute For Biological Studies Methods and compositions for treating neurological disorders
US9845457B2 (en) 2010-04-30 2017-12-19 Cedars-Sinai Medical Center Maintenance of genomic stability in cultured stem cells
US9249392B2 (en) 2010-04-30 2016-02-02 Cedars-Sinai Medical Center Methods and compositions for maintaining genomic stability in cultured stem cells
WO2011146862A1 (en) 2010-05-21 2011-11-24 Bellicum Pharmaceuticals, Inc. Methods for inducing selective apoptosis
EP2601289B1 (en) 2010-08-04 2017-07-12 Cellular Dynamics International, Inc. Reprogramming immortalized b cells
CA2812194C (en) 2010-09-17 2022-12-13 President And Fellows Of Harvard College Functional genomics assay for characterizing pluripotent stem cell utility and safety
US9637732B2 (en) 2010-11-04 2017-05-02 Kyoto University Method of efficiently establishing induced pluripotent stem cells
JP6029137B2 (ja) 2010-12-03 2016-11-24 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
WO2012115270A1 (ja) * 2011-02-25 2012-08-30 学校法人慶應義塾 ｉＰＳ細胞クローンの選択方法、及びその選択方法に用いる遺伝子の選択方法
CN102731653B (zh) * 2011-04-06 2013-12-11 李凌松 一种抗磷酸化Oct4蛋白的抗体及其应用
GB2496375A (en) 2011-10-28 2013-05-15 Kymab Ltd A non-human assay vertebrate comprising human antibody loci and human epitope knock-in, and uses thereof
GB201122047D0 (en) 2011-12-21 2012-02-01 Kymab Ltd Transgenic animals
CN111893140A (zh) 2011-12-05 2020-11-06 菲克特生物科学股份有限公司 用于转染细胞的方法和产品
US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
WO2013109763A2 (en) * 2012-01-17 2013-07-25 The Board Of Trustees Of The Leland Stanford Junior University Activation of innate immunity for nuclear reprogramming of somatic cells
US11963977B2 (en) * 2012-04-24 2024-04-23 Vcell Therapeutics Inc. Generating pluripotent cells de novo
WO2013184527A1 (en) 2012-06-05 2013-12-12 Capricor, Inc. Optimized methods for generation of cardiac stem cells from cardiac tissue and their use in cardiac therapy
JP6433896B2 (ja) 2012-08-13 2018-12-05 シーダーズ−サイナイ・メディカル・センターＣｅｄａｒｓ−Ｓｉｎａｉ Ｍｅｄｉｃａｌ Ｃｅｎｔｅｒ 組織再生のためのエキソソームおよびマイクロリボ核酸
JPWO2014057997A1 (ja) 2012-10-09 2016-09-05 仲信 林 初期化ペプチド及びその用途
US20140287932A1 (en) 2012-10-25 2014-09-25 Whitehead Institute For Biomedical Research Super-enhancers and methods of use thereof
KR102315098B1 (ko) 2012-11-01 2021-10-21 팩터 바이오사이언스 인크. 세포에서 단백질을 발현시키는 방법들과 생성물들
EP2948776B8 (en) 2013-01-25 2020-02-26 Xcell Biosciences, Inc. Methods, compositions, kits, and systems for selective enrichment of target cells
US9434935B2 (en) 2013-03-10 2016-09-06 Bellicum Pharmaceuticals, Inc. Modified caspase polypeptides and uses thereof
CA2903025C (en) * 2013-03-11 2025-10-07 Regeneron Pharmaceuticals, Inc. TRANSGENIC MICE EXPRESSING CHIMERIC MAJOR HISTOCOMPATIBILITY COMPLEX (MHC) CLASS II MOLECULES
EP3736343A1 (en) 2013-03-15 2020-11-11 Whitehead Institute For Biomedical Research Cellular discovery platform for neurodegenerative diseases
JP2016521971A (ja) 2013-04-23 2016-07-28 イェダ リサーチ アンド ディベロップメント カンパニー リミテッド 単離ナイーブ型多能性幹細胞およびそれを発生させる方法関連出願本出願は、米国特許法１１９条第（ｅ）項に基づき、２０１４年１月２９日出願の米国特許仮出願第６１／９３２，９３５号、２０１３年９月１７日出願の米国特許仮出願第６１／８７８，７６９号、および２０１３年４月２３日出願の米国特許仮出願第６１／８１４，９２０号の優先権を主張する。また、本出願は、同時に提出された同出願人による同時係属出願である、ＹａｑｕｂＨＡＮＮＡ、ＮｏａＮＯＶＥＲＳＨＴＥＲＮ、およびＹｏａｃｈＲＡＩＳによる米国特許出願（発明の名称「単離ナイーブ型多能性幹細胞およびそれを発生させる方法（ＩＳＯＬＡＴＥＤＮＡＩＶＥＰＬＵＲＩＰＯＴＥＮＴＳＴＥＭＣＥＬＬＳＡＮＤＭＥＴＨＯＤＳＯＦＧＥＮＥＲＡＴＩＮＧＳＡＭＥ）」）（代理人事件記録簿第５８８７０号）にも関する。上記出願の内容はその全体を参考として本明細書に組み込む。
US9913882B2 (en) 2013-06-05 2018-03-13 Bellicum Pharmaceuticals, Inc. Methods for inducing partial apoptosis using caspase polypeptides
EP3008229B1 (en) 2013-06-10 2020-05-27 President and Fellows of Harvard College Early developmental genomic assay for characterizing pluripotent stem cell utility and safety
WO2015037535A1 (ja) 2013-09-12 2015-03-19 株式会社カネカ 人工多能性幹細胞の分化誘導方法及び選別方法
WO2015069736A1 (en) 2013-11-08 2015-05-14 The Mclean Hospital Corporation METHODS FOR EFFICIENT GENERATION OF GABAergic INTERNEURONS FROM PLURIPOTENT STEM CELLS
EP3690056B1 (en) 2014-01-31 2022-12-28 Factor Bioscience Inc. Methods and products for nucleic acid production and delivery
WO2015120225A1 (en) 2014-02-10 2015-08-13 The Board Of Trustees Of The Leland Stanford Junior University ACTIVATION OF INNATE IMMUNITY FOR ENHANCED NUCLEAR REPROGRAMMING OF SOMATIC CELLS WITH mRNA
RU2711942C2 (ru) 2014-03-19 2020-01-23 Виселл Терапеутикс, Инк. Способы, относящиеся к плюрипотентным клеткам
EP3194572B1 (en) 2014-07-30 2023-10-25 Yeda Research and Development Co. Ltd. Media for culturing pluripotent stem cells
US11261453B2 (en) 2014-09-12 2022-03-01 Whitehead Institute For Biomedical Research Cells expressing apolipoprotein E and uses thereof
JP6878274B2 (ja) 2014-10-03 2021-05-26 シーダーズ−サイナイ・メディカル・センターＣｅｄａｒｓ−Ｓｉｎａｉ Ｍｅｄｉｃａｌ Ｃｅｎｔｅｒ 筋ジストロフィーの処置における心筋球由来細胞およびこのような細胞によって分泌されたエキソソーム
WO2016123117A1 (en) 2015-01-26 2016-08-04 Fate Therapeutics, Inc. Cells with increased immuno-regulatory properties and methods for their use and manufacture
CA2976376A1 (en) 2015-02-13 2016-08-18 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
EP3283502A4 (en) * 2015-04-07 2019-04-03 The General Hospital Corporation METHOD FOR REACTIVATING GENES ON THE INACTIVE X-CHROMOSOME
EP3708680B8 (en) * 2015-04-15 2023-08-30 University of Massachusetts Compositions and methods for xi chromosome reactivation
EP3286310A4 (en) 2015-04-24 2019-01-09 California Institute of Technology REACTIVATION OF X-CHROMOSOME GENES
US20170044609A1 (en) * 2015-04-24 2017-02-16 California Institute Of Technology Reactivation of x chromosome genes
CA2993201A1 (en) 2015-07-21 2017-01-26 The Children's Medical Center Corporation Pd-l1 expressing hematopoietic stem cells and uses
US10662409B2 (en) * 2015-09-09 2020-05-26 Trustees Of Tufts College Methods of generating neural stem cells
WO2017123662A1 (en) 2016-01-11 2017-07-20 Cedars-Sinai Medical Center Cardiosphere-derived cells and exosomes secreted by such cells in the treatment of heart failure with preserved ejection fraction
CN105624117A (zh) * 2016-01-18 2016-06-01 哈尔滨医科大学 一种低氧促进小鼠成纤维细胞重编程为心肌细胞的方法
WO2017210652A1 (en) 2016-06-03 2017-12-07 Cedars-Sinai Medical Center Cdc-derived exosomes for treatment of ventricular tachyarrythmias
WO2017223199A1 (en) * 2016-06-22 2017-12-28 Xcell Biosciences, Inc. Methods for increasing cell culture transfection efficiency and cellular reprogramming
AU2017312113B2 (en) 2016-08-17 2023-05-25 Factor Bioscience Inc. Nucleic acid products and methods of administration thereof
CA3034369A1 (en) 2016-08-19 2018-02-22 Whitehead Institute For Biomedical Research Methods of editing dna methylation
EP3515459A4 (en) 2016-09-20 2020-08-05 Cedars-Sinai Medical Center CELLS DERIVED FROM CARDIOSPHERES AND THEIR EXTRACELLULAR VESICLES TO DELAY OR REVERSE AGING AND AGE-RELATED DISORDERS
CN106544315A (zh) * 2016-10-12 2017-03-29 广东艾时代生物科技有限责任公司 一种脂肪间充质干细胞诱导成多能干细胞的方法
JP2019537427A (ja) 2016-10-27 2019-12-26 カリフォルニア インスティチュート オブ テクノロジー Ｘ染色体の再活性化のためのｈｄａｃ阻害剤組成物
AU2017375652B2 (en) 2016-12-16 2024-08-15 Iviva Medical, Inc. Thin film interposition for basement membrane scaffolds
US11873496B2 (en) 2017-01-09 2024-01-16 Whitehead Institute For Biomedical Research Methods of altering gene expression by perturbing transcription factor multimers that structure regulatory loops
JPWO2018159805A1 (ja) 2017-03-03 2020-01-09 国立大学法人京都大学 膵前駆細胞の製造方法
WO2018185709A1 (en) * 2017-04-05 2018-10-11 Centro De Neurociencias E Biologia Celular Compositions for reprogramming cells into dendritic cells or antigen presenting cells, methods and uses thereof
CA3059910A1 (en) 2017-04-19 2018-10-25 Cedars-Sinai Medical Center Methods and compositions for treating skeletal muscular dystrophy
JPWO2018230588A1 (ja) 2017-06-14 2020-04-16 武田薬品工業株式会社 細胞封入デバイス
WO2019060708A1 (en) 2017-09-22 2019-03-28 The Children's Medical Center Corporation TREATMENT OF TYPE 1 DIABETES AND AUTOIMMUNE DISEASES OR DISORDERS
PL241125B1 (pl) 2017-11-30 2022-08-08 Gdanski Univ Medyczny Kompozycja farmaceutyczna do zastosowania jako środek do pobudzania regeneracji lub gojenia ran
TW201940693A (zh) 2017-11-30 2019-10-16 國立大學法人京都大學 細胞之培養方法
EP3727351A4 (en) 2017-12-20 2021-10-06 Cedars-Sinai Medical Center MANIPULATED EXTRACELLULAR VESICLES FOR ENHANCED RELEASE INTO TISSUE
TWI821230B (zh) 2017-12-22 2023-11-11 日商千紙鶴治療公司 細胞培養裝置、培養液吸引器及細胞培養方法
CN108624621B (zh) * 2018-01-17 2019-04-12 中国科学院上海生命科学研究院 非人灵长类的体细胞克隆动物的制备方法
CN108103027B (zh) * 2018-02-02 2021-12-24 中国医学科学院血液病医院（血液学研究所） 高效率血细胞重编程同时实现基因编辑的方法
WO2019152549A1 (en) 2018-02-05 2019-08-08 Cedars-Sinai Medical Center Methods for therapeutic use of exosomes and y-rnas
TW201945536A (zh) 2018-03-19 2019-12-01 國立大學法人京都大學 水凝膠膠囊
WO2019189553A1 (ja) 2018-03-30 2019-10-03 国立大学法人京都大学 複素環化合物
CN111918961B (zh) 2018-03-30 2023-10-24 国立大学法人京都大学 心肌细胞成熟促进剂
WO2019189545A1 (ja) 2018-03-30 2019-10-03 国立大学法人京都大学 細胞の製造方法
CN112313327B (zh) 2018-04-23 2024-04-19 千纸鹤治疗公司 增殖抑制剂
SG11202101098YA (en) 2018-08-03 2021-03-30 Univ Kyoto Cell production method
WO2020032185A1 (ja) 2018-08-10 2020-02-13 国立大学法人京都大学 カチオン性脂質を用いた心筋細胞へのトランスフェクション方法
WO2020032179A1 (ja) 2018-08-10 2020-02-13 国立大学法人京都大学 Ｃｄ３陽性細胞の製造方法
BR112021003092A2 (pt) 2018-08-22 2021-05-11 Kyoto University métodos para produzir precursores neurais entéricos, para produzir um organoide intestinal, para produzir um trato intestinal artificial e de expansão de cultura para precursores neurais entéricos, precursores neurais entéricos, estoque congelado, medicamento de célula, e, meio de precursor neural entérico
SG11202101930XA (en) 2018-08-31 2021-03-30 Noile Immune Biotech Inc Car-expressing t cells and car expression vector
TWI862513B (zh) 2018-09-19 2024-11-21 日商千紙鶴治療公司 包含胰島素產生細胞之細胞集團、包含該細胞集團之醫藥、其用途及製造方法
CA3113676A1 (en) 2018-09-21 2020-03-26 President And Fellows Of Harvard College Methods and compositions for treating diabetes, and methods for enriching mrna coding for secreted proteins
US20220135938A1 (en) 2018-10-15 2022-05-05 Public University Corporation Yokohama City University Nutrition composition
TW202039543A (zh) 2018-12-27 2020-11-01 國立大學法人京都大學 Ｔ細胞受體的變體
WO2020150391A1 (en) * 2019-01-15 2020-07-23 Falcon Therapeutics, Inc. Engineered stem cell constructs and uses thereof
CN113382768A (zh) 2019-02-01 2021-09-10 国立大学法人京都大学 细胞的检测方法
WO2020204149A1 (ja) 2019-03-29 2020-10-08 公立大学法人横浜市立大学 スクリーニング方法および毒性評価法
SG11202111123SA (en) 2019-04-10 2021-11-29 Orizuru Therapeutics Inc Method for producing biological tissue-like structure
EP3969571A4 (en) 2019-05-14 2023-05-24 Aleph Farms Ltd. AGGREGATES OF PLURIPOTENT CELLS AND THEIR USE
EP3985104A4 (en) 2019-06-11 2023-04-12 Kyoto University PROCEDURE FOR GENERATING A KIDNEY INTERSTITIAL CELL
EP3997059A4 (en) 2019-07-03 2023-12-13 Factor Bioscience Inc. CATIONIC LIPIDS AND THEIR USES
US10501404B1 (en) 2019-07-30 2019-12-10 Factor Bioscience Inc. Cationic lipids and transfection methods
AU2020332643A1 (en) 2019-08-20 2022-03-10 Orizuru Therapeutics, Inc. Method for enriching cardiac myocytes
JP7709737B2 (ja) 2019-10-21 2025-07-17 オリヅルセラピューティクス株式会社 増殖抑制剤
CN114729318B (zh) 2019-11-01 2025-03-11 国立大学法人京都大学 T细胞的制备方法
EP4067490A4 (en) 2019-11-25 2024-02-21 Kyoto University T-cell master cell bank
CN115427552A (zh) 2020-03-19 2022-12-02 千纸鹤治疗公司 心肌细胞的精制方法
IL296317A (en) 2020-03-19 2022-11-01 Orizuru Therapeutics Inc Cardiomyocyte purification method
TW202204609A (zh) 2020-03-31 2022-02-01 國立大學法人京都大學 Ｔ前驅細胞的製造方法
CN115803426A (zh) 2020-05-28 2023-03-14 千纸鹤治疗公司 均一尺寸的细胞聚集体的大量制造方法
WO2022107877A1 (ja) 2020-11-20 2022-05-27 オリヅルセラピューティクス株式会社 成熟化剤
KR20230145101A (ko) 2021-02-09 2023-10-17 오리즈루 세라퓨틱스 가부시키가이샤 성숙화제
WO2022191171A1 (ja) 2021-03-09 2022-09-15 国立大学法人 東京医科歯科大学 細胞クラスターの製造方法
AU2022255421A1 (en) 2021-04-08 2023-10-26 Noile-Immune Biotech Inc. Method for activating t-cells
WO2022230919A1 (ja) 2021-04-28 2022-11-03 国立大学法人 東京医科歯科大学 細胞の製造方法
CN113278686A (zh) * 2021-06-11 2021-08-20 扬州大学 一种研究dna甲基化调控重编程过程的方法
AU2022349176A1 (en) 2021-09-27 2024-04-11 Kyoto University Method for producing t cell
EP4474476A1 (en) 2022-02-04 2024-12-11 Kyoto University T cell production method
CN119256076A (zh) 2022-03-23 2025-01-03 国立大学法人京都大学 用于产生调节性t细胞的方法
IL315122A (en) 2022-04-25 2024-10-01 Orizuru Therapeutics Inc Maturing agent having both of alk5 inhibitory activity and cdk8/19 inhibitory activity
JPWO2023243627A1 (enExample) 2022-06-17 2023-12-21
IL318270A (en) 2022-07-14 2025-03-01 Orizuru Therapeutics Inc Fibrin gel sheet for cell transplantation
WO2024024742A1 (ja) 2022-07-25 2024-02-01 国立大学法人京都大学 霊長類の胎児卵巣細胞から卵胞を誘導する体外培養法
EP4563703A4 (en) 2022-07-26 2025-09-24 Univ Kyoto ARTIFICIAL RECEIVER HAVING A LOAD-SHEDDING STRUCTURE
WO2024029617A1 (ja) 2022-08-05 2024-02-08 国立大学法人京都大学 心筋の製造方法
EP4570902A1 (en) 2022-08-08 2025-06-18 Healios K.K. Method for producing cell aggregates
WO2024050517A1 (en) * 2022-09-02 2024-03-07 The University Of North Carolina At Chapel Hill Methods of making and using anticancer reprogrammed b cells
EP4596675A1 (en) 2022-09-26 2025-08-06 Kyoto University Method for producing pancreatic endoderm cells
EP4600352A1 (en) 2022-09-26 2025-08-13 Kyoto University T cell production method
JPWO2024071280A1 (enExample) 2022-09-29 2024-04-04
JPWO2024106444A1 (enExample) 2022-11-16 2024-05-23
WO2025038494A1 (en) 2023-08-11 2025-02-20 Tune Therapeutics, Inc. Compositions, systems, and methods for lymphoid cell differentiation using targeted gene activation
WO2025059073A1 (en) 2023-09-11 2025-03-20 Tune Therapeutics, Inc. Epigenetic editing methods and systems for differentiating stem cells
WO2025106897A1 (en) * 2023-11-16 2025-05-22 Colossal Biosciences Inc. Methods and compositions for creating induced pluripotent stem cells
WO2025260068A1 (en) 2024-06-14 2025-12-18 Tune Therapeutics, Inc. Lipid nanoparticle formulation for delivery of nucleic acids to cells

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL68218A (en) 1983-03-23 1985-12-31 Univ Ramot Compositions for cartilage repair comprising embryonal chondrocytes
US5041138A (en) 1986-11-20 1991-08-20 Massachusetts Institute Of Technology Neomorphogenesis of cartilage in vivo from cell culture
US4846835A (en) 1987-06-15 1989-07-11 Grande Daniel A Technique for healing lesions in cartilage
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
WO1993010218A1 (en) 1991-11-14 1993-05-27 The United States Government As Represented By The Secretary Of The Department Of Health And Human Services Vectors including foreign genes and negative selective markers
US5646008A (en) 1993-06-22 1997-07-08 The Regent Of The University Of Michigan Vertebrate apoptosis gene: compositions and methods
JPH09500534A (ja) * 1993-07-22 1997-01-21 メルク エンド カンパニー インコーポレーテッド トランスジェニック動物でのヒトインターロイキン―１βの発現
US5723331A (en) 1994-05-05 1998-03-03 Genzyme Corporation Methods and compositions for the repair of articular cartilage defects in mammals
US5698446A (en) 1994-09-07 1997-12-16 Chiron Corporation Methods and compositions for inhibiting production of replication competent virus
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
GB9517779D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
GB9809178D0 (en) 1998-04-29 1998-07-01 Univ Edinburgh Nuclear reprogramming of somatic cells
EP1129176A4 (en) 1998-11-09 2002-10-30 Es Cell Int Pte Ltd EMBRYONIC STEM CELLS
JP4523169B2 (ja) * 1999-02-04 2010-08-11 プルリステム リミテッド 造血幹細胞および／または前駆細胞を維持および増加するための方法および装置
US7015037B1 (en) * 1999-08-05 2006-03-21 Regents Of The University Of Minnesota Multiponent adult stem cells and methods for isolation
US20020168660A1 (en) 2001-02-15 2002-11-14 Whitehead Institute For Biomedical Research Stem cell self-renewal and lineage commitment
US20020137460A1 (en) * 2001-03-08 2002-09-26 Howard Sun Universal multi-band (5 bands and more) keyless RF remote control unit using bluetooth radio module as the base
AU2002306374B2 (en) 2001-05-31 2008-01-24 Dainippon Sumitomo Pharma Co., Ltd. Genes with ES cell-specific expression
JP2003033179A (ja) 2001-07-05 2003-02-04 Asahi Kasei Corp 可逆的遺伝子導入ベクター
US20050130144A1 (en) 2001-09-21 2005-06-16 Norio Nakatsuji Method of screening reprogramming factor, reprogramming factor screened by the method, method of using the reprogramming factor, method of differentiating undifferentiated fused cells and method of constructing cell, tissues and organs
DE10162080A1 (de) * 2001-12-10 2003-06-26 Albrecht Mueller Verfahren zur Herstellung von Stammzellen mit erhöhtem Entwicklungspotential
GB0202149D0 (en) * 2002-01-30 2002-03-20 Univ Edinburgh Pluripotency determining factors and uses thereof
GB0211904D0 (en) * 2002-05-23 2002-07-03 Medical Res Council Nuclear transfer
EP1546346A4 (en) 2002-07-23 2006-02-08 Nanodiagnostics Inc MARKERS OF EMBRYONIC STEM CELLS, AND USE THEREOF
AU2004252571C1 (en) * 2003-06-27 2012-03-01 Ethicon, Incorporated Postpartum-derived cells for use in treatment of disease of the heart and circulatory system
US7682828B2 (en) 2003-11-26 2010-03-23 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
WO2005080598A1 (ja) * 2004-02-19 2005-09-01 Dainippon Sumitomo Pharma Co., Ltd. 体細胞核初期化物質のスクリーニング方法
EP1574499A1 (en) 2004-03-08 2005-09-14 DKFZ Deutsches Krebsforschungszentrum Inhibitors of DNA methylation in tumor cells
RU2375448C2 (ru) * 2004-03-23 2009-12-10 Асубио Фарма Ко., Лтд. Способ выращивания плюрипотентных стволовых клеток
US20060148104A1 (en) 2004-10-29 2006-07-06 Massachusetts Institute Of Technology Detection of ion channel or receptor activity
WO2006116803A1 (en) * 2005-04-29 2006-11-09 Innovative Dairy Products Pty Ltd As Trustee For The Participants Of The Cooperative Research Centre For Innovative Dairy Products A method for producing stem cells or stem cell-like cells from mammalian embryos
WO2007007054A1 (en) 2005-07-08 2007-01-18 Cancer Research Technology Limited Phthalamides, succinimides and related compounds and their use as pharmaceuticals
JP4395193B2 (ja) * 2005-08-01 2010-01-06 ニューポテンシャル，インコーポレイテッド 回復能を有する再プログラムされた細胞の作製
US20090227032A1 (en) * 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
ZA200804673B (en) * 2005-12-13 2009-11-25 Univ Kyoto Nuclear reprogramming factor
US8278104B2 (en) * 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
GB0615327D0 (en) * 2006-03-30 2006-09-13 Univ Edinburgh Culture medium containing kinase inhibitors and uses thereof
JP2009542626A (ja) 2006-06-27 2009-12-03 ジャワハーラル ネール センター フォー アドヴァンスド サイエンティフィック リサーチ メチルトランスフェラーゼ（ｈｍｔａｓｅ）の部位特異的阻害剤及びその製剤方法
WO2008118820A2 (en) * 2007-03-23 2008-10-02 Wisconsin Alumni Research Foundation Somatic cell reprogramming
CA2683056C (en) 2007-04-07 2020-03-24 Whitehead Institute For Biomedical Research Reprogramming of somatic cells
US20120282229A1 (en) * 2007-08-01 2012-11-08 Christian Kannemeier Non-viral delivery of transcription factors that reprogram human somatic cells into a stem cell-like state
MX2010002242A (es) * 2007-08-31 2010-06-01 Whitehead Biomedical Inst Estimulación de ruta wnt en reprogramacion de celulas somaticas.
WO2009061442A1 (en) 2007-11-06 2009-05-14 Children's Medical Center Corporation Method to produce induced pluripotent stem (ips) cells form non-embryonic human cells
SG188904A1 (en) 2008-03-17 2013-04-30 Scripps Research Inst Combined chemical and genetic approaches for generation of induced pluripotent stem cells
JP2011516042A (ja) 2008-03-17 2011-05-26 へルムホルツ・ツェントルム・ミュンヘン−ドイチェス・フォルシュングスツェントルム・ヒューア・ゲズントハイト・ウント・ウムヴェルト（ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング） 部位特異的組み換えを用いて無ベクター誘導多能性幹（ｉＰＳ）細胞を作製するベクターおよびその方法
US20100279404A1 (en) 2008-05-02 2010-11-04 Shinya Yamanaka Method of nuclear reprogramming
CA3060170A1 (en) 2008-06-13 2009-12-17 Whitehead Institute For Biomedical Research Programming and reprogramming of cells
US20120034192A1 (en) * 2008-09-19 2012-02-09 Young Richard A Compositions and methods for enhancing cell reprogramming
US20100150889A1 (en) 2008-12-17 2010-06-17 The Uab Research Foundation Polycistronic Vector For Human Induced Pluripotent Stem Cell Production

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
RAMJI DP 等: "CCAAT/enhancer-binding proteins structure, function and regulation", 《BIOCHEM J》 *
TAKAHASHI K 等: "Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors", 《CELL》 *
XIE H 等: "Stepwise Reprogramming of B Cells into Macrophages", 《CELL》 *
陈赛娟 主编: "《血液恶性疾病基因异常和靶向治疗》", 31 January 2006 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112201307A (zh) * 2020-09-23 2021-01-08 温州医科大学 一种转录本注释方法以及筛选长非编码rna和内源逆转录病毒来源长非编码rna的方法
CN113424794A (zh) * 2021-04-16 2021-09-24 安徽农业大学 一种优质抗病型地方鸡新品系选育方法及应用

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