CH676990A5 - - Google Patents
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- Publication number
- CH676990A5 CH676990A5 CH4680/86A CH468086A CH676990A5 CH 676990 A5 CH676990 A5 CH 676990A5 CH 4680/86 A CH4680/86 A CH 4680/86A CH 468086 A CH468086 A CH 468086A CH 676990 A5 CH676990 A5 CH 676990A5
- Authority
- CH
- Switzerland
- Prior art keywords
- superoxide dismutase
- human
- sep
- mnsod
- plasmid
- Prior art date
Links
- 102000019197 Superoxide Dismutase Human genes 0.000 abstract description 249
- 108010012715 Superoxide dismutase Proteins 0.000 abstract description 249
- 241000282414 Homo sapiens Species 0.000 abstract description 205
- 238000000034 method Methods 0.000 abstract description 86
- 101000868115 Homo sapiens Superoxide dismutase [Mn], mitochondrial Proteins 0.000 abstract description 51
- 239000011572 manganese Substances 0.000 abstract description 50
- 230000001580 bacterial effect Effects 0.000 abstract description 45
- 239000002609 medium Substances 0.000 abstract description 37
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract description 25
- 239000013587 production medium Substances 0.000 abstract description 21
- 210000000056 organ Anatomy 0.000 abstract description 15
- 206010061218 Inflammation Diseases 0.000 abstract description 7
- 230000004054 inflammatory process Effects 0.000 abstract description 7
- 230000009467 reduction Effects 0.000 abstract description 5
- 230000004083 survival effect Effects 0.000 abstract description 5
- 206010063837 Reperfusion injury Diseases 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 126
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- 102000004196 processed proteins & peptides Human genes 0.000 description 116
- 108090000765 processed proteins & peptides Proteins 0.000 description 116
- 239000013612 plasmid Substances 0.000 description 108
- 108090000623 proteins and genes Proteins 0.000 description 94
- 101710119418 Superoxide dismutase [Mn] Proteins 0.000 description 91
- 101710202572 Superoxide dismutase [Mn], mitochondrial Proteins 0.000 description 91
- 102000004169 proteins and genes Human genes 0.000 description 78
- 235000018102 proteins Nutrition 0.000 description 76
- 102100032891 Superoxide dismutase [Mn], mitochondrial Human genes 0.000 description 75
- 108020004414 DNA Proteins 0.000 description 72
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- 229910001437 manganese ion Inorganic materials 0.000 description 44
- 125000003275 alpha amino acid group Chemical group 0.000 description 41
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 40
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- 108090000790 Enzymes Proteins 0.000 description 29
- 229940088598 enzyme Drugs 0.000 description 29
- 238000010367 cloning Methods 0.000 description 28
- 102000053602 DNA Human genes 0.000 description 26
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 26
- 239000000463 material Substances 0.000 description 26
- 239000000825 pharmaceutical preparation Substances 0.000 description 26
- 239000001963 growth medium Substances 0.000 description 24
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 22
- 101150017120 sod gene Proteins 0.000 description 22
- 235000011056 potassium acetate Nutrition 0.000 description 20
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 18
- 239000004472 Lysine Substances 0.000 description 18
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- 230000001965 increasing effect Effects 0.000 description 16
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- 108091026890 Coding region Proteins 0.000 description 14
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 14
- 229910052802 copper Inorganic materials 0.000 description 14
- 239000010949 copper Substances 0.000 description 14
- 239000006228 supernatant Substances 0.000 description 14
- TUGDLVFMIQZYPA-UHFFFAOYSA-N tetracopper;tetrazinc Chemical compound [Cu+2].[Cu+2].[Cu+2].[Cu+2].[Zn+2].[Zn+2].[Zn+2].[Zn+2] TUGDLVFMIQZYPA-UHFFFAOYSA-N 0.000 description 14
- 241000894006 Bacteria Species 0.000 description 12
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical class [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 12
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- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 8
- 239000006137 Luria-Bertani broth Substances 0.000 description 8
- 239000004098 Tetracycline Substances 0.000 description 8
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- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
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- 238000007792 addition Methods 0.000 description 6
- 229960000723 ampicillin Drugs 0.000 description 6
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 6
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- 239000011701 zinc Substances 0.000 description 6
- 229910052725 zinc Inorganic materials 0.000 description 6
- QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 4
- 108010077805 Bacterial Proteins Proteins 0.000 description 4
- 102000004594 DNA Polymerase I Human genes 0.000 description 4
- 108010017826 DNA Polymerase I Proteins 0.000 description 4
- SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 4
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- 108020004511 Recombinant DNA Proteins 0.000 description 4
- 238000012300 Sequence Analysis Methods 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 4
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- 229910052708 sodium Inorganic materials 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 4
- 238000010186 staining Methods 0.000 description 4
- -1 superoxide anions Chemical class 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 230000001225 therapeutic effect Effects 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 4
- 210000005253 yeast cell Anatomy 0.000 description 4
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 208000005623 Carcinogenesis Diseases 0.000 description 2
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- 241001646716 Escherichia coli K-12 Species 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
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- 108010063312 Metalloproteins Proteins 0.000 description 2
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- 239000000020 Nitrocellulose Substances 0.000 description 2
- 102000004316 Oxidoreductases Human genes 0.000 description 2
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 2
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- 241000219061 Rheum Species 0.000 description 2
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- 239000005018 casein Substances 0.000 description 2
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 2
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- 210000000224 granular leucocyte Anatomy 0.000 description 2
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- 150000004678 hydrides Chemical class 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
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- 210000000265 leukocyte Anatomy 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
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- 230000033001 locomotion Effects 0.000 description 2
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- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 230000036244 malformation Effects 0.000 description 2
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 230000000926 neurological effect Effects 0.000 description 2
- 229920001220 nitrocellulos Polymers 0.000 description 2
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 2
- 231100000989 no adverse effect Toxicity 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 230000001590 oxidative effect Effects 0.000 description 2
- 230000004783 oxidative metabolism Effects 0.000 description 2
- 230000000242 pagocytic effect Effects 0.000 description 2
- 210000001539 phagocyte Anatomy 0.000 description 2
- 229940127557 pharmaceutical product Drugs 0.000 description 2
- 229910000160 potassium phosphate Inorganic materials 0.000 description 2
- 235000011009 potassium phosphates Nutrition 0.000 description 2
- 210000001236 prokaryotic cell Anatomy 0.000 description 2
- 235000013930 proline Nutrition 0.000 description 2
- 125000001500 prolyl group Chemical class [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 2
- 230000002685 pulmonary effect Effects 0.000 description 2
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- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 229920002477 rna polymer Polymers 0.000 description 2
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- 239000011780 sodium chloride Substances 0.000 description 2
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/44—Oxidoreductases (1)
- A61K38/446—Superoxide dismutase (1.15)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/08—Vasodilators for multiple indications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Genetics & Genomics (AREA)
- Heart & Thoracic Surgery (AREA)
- Cardiology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Urology & Nephrology (AREA)
- Vascular Medicine (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Epidemiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US80109085A | 1985-11-22 | 1985-11-22 | |
| US90705186A | 1986-09-12 | 1986-09-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CH676990A5 true CH676990A5 (enrdf_load_stackoverflow) | 1991-03-28 |
Family
ID=27122291
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CH4680/86A CH676990A5 (enrdf_load_stackoverflow) | 1985-11-22 | 1986-11-21 |
Country Status (21)
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0587055A1 (de) * | 1992-09-09 | 1994-03-16 | BOEHRINGER INGELHEIM INTERNATIONAL GmbH | Verwendung von Mangan-Superoxiddismutase (Mn-SOD) zur Herstellung von Arzneimitteln zur Behandlung von Erkrankungen in niedriger Dosierung |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6610520B1 (en) * | 1985-11-22 | 2003-08-26 | Bio-Technology General Corp. | Gene encoding human manganese superoxide dismutase and recombinant polypeptide encoded thereby |
| JPS62215532A (ja) * | 1986-03-18 | 1987-09-22 | Ube Ind Ltd | 抗炎症剤 |
| JPH0643341B2 (ja) * | 1986-03-27 | 1994-06-08 | 宇部興産株式会社 | 臓器機能改善剤 |
| JPH0643340B2 (ja) * | 1986-09-03 | 1994-06-08 | 宇部興産株式会社 | 虚血性心疾患治療薬 |
| JPS6377822A (ja) * | 1986-09-18 | 1988-04-08 | Ube Ind Ltd | 臓器機能改善剤 |
| US6326003B1 (en) | 1986-10-14 | 2001-12-04 | Chiron Corporation | Manganese superoxide dismutase cloning and expression in microorganisms |
| ATE132902T1 (de) * | 1987-03-14 | 1996-01-15 | Boehringer Ingelheim Int | Humane mangan-superoxiddismutase (hmn-sod) |
| US5260204A (en) * | 1987-03-14 | 1993-11-09 | Boehringer Ingelheim International Gmbh | Human manganese superoxide dismutase (hMn-SOD) |
| EP0284105B1 (en) * | 1987-03-27 | 1995-11-15 | Bio-Technology General Corporation | Human manganese superoxide dismutase and methods of treatment |
| JPH01233228A (ja) * | 1988-03-11 | 1989-09-19 | Toyo Jozo Co Ltd | 悪性腫瘍細胞転移防止剤 |
| US5772996A (en) * | 1990-08-03 | 1998-06-30 | Public Health Laboratory Service Board | Pharmaceutical compositions containing superoxide dismutase from Bacillus Stearothermophilus and Bacillus Caldotenax |
| DE4038563A1 (de) * | 1990-12-04 | 1992-06-11 | Gruenenthal Gmbh | Verwendung von superoxiddismutasen zur prophylaxe und/oder behandlung von organversagen bei risikopatienten mit polytrauma |
| DE4239877C1 (de) * | 1992-11-27 | 1994-03-17 | Boehringer Ingelheim Int | Stabilisierte Superoxid-Dismutase (SOD)-Zusammensetzung |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56102787A (en) * | 1980-01-18 | 1981-08-17 | Mamoru Sugiura | Preparation of human placenta superoxide dismutase |
| BG49718A3 (bg) * | 1983-07-15 | 1992-01-15 | Bio- Technology General Corp | Метод за получаване на полипептид със супероксиддисмутазна активност |
| DE3485284D1 (de) * | 1983-10-03 | 1992-01-02 | Chiron Corp | Klonieren von superoxid-dismutase und expression in mikroorganismen. |
-
1986
- 1986-10-29 IE IE285186A patent/IE59498B1/en not_active IP Right Cessation
- 1986-11-07 DK DK534686A patent/DK534686A/da not_active Application Discontinuation
- 1986-11-10 AU AU64975/86A patent/AU607897B2/en not_active Ceased
- 1986-11-13 GR GR862713A patent/GR862713B/el unknown
- 1986-11-14 SE SE8604884A patent/SE8604884L/xx not_active Application Discontinuation
- 1986-11-14 GB GB8627294A patent/GB2183658B/en not_active Expired - Fee Related
- 1986-11-18 ES ES8603083A patent/ES2003518A6/es not_active Expired
- 1986-11-19 AT AT0308986A patent/AT400443B/de not_active IP Right Cessation
- 1986-11-20 BE BE0/217445A patent/BE905796A/fr not_active IP Right Cessation
- 1986-11-20 IL IL80702A patent/IL80702A0/xx unknown
- 1986-11-20 IL IL10644986A patent/IL106449A/xx active IP Right Grant
- 1986-11-20 LU LU86676A patent/LU86676A1/fr unknown
- 1986-11-21 CA CA000523536A patent/CA1341362C/en not_active Expired - Lifetime
- 1986-11-21 CH CH4680/86A patent/CH676990A5/de not_active IP Right Cessation
- 1986-11-21 JP JP61278528A patent/JP2609449B2/ja not_active Expired - Lifetime
- 1986-11-21 FI FI864756A patent/FI90353C/fi not_active IP Right Cessation
- 1986-11-21 IT IT67865/86A patent/IT1205418B/it active
- 1986-11-21 DE DE3639725A patent/DE3639725C2/de not_active Expired - Fee Related
- 1986-11-21 NL NL8602960A patent/NL8602960A/nl active Search and Examination
- 1986-11-21 FR FR868616238A patent/FR2590591B1/fr not_active Expired
- 1986-11-21 PT PT83792A patent/PT83792B/pt not_active IP Right Cessation
-
1995
- 1995-05-18 HK HK75195A patent/HK75195A/xx not_active IP Right Cessation
-
1996
- 1996-01-22 JP JP8008627A patent/JP2967557B2/ja not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0587055A1 (de) * | 1992-09-09 | 1994-03-16 | BOEHRINGER INGELHEIM INTERNATIONAL GmbH | Verwendung von Mangan-Superoxiddismutase (Mn-SOD) zur Herstellung von Arzneimitteln zur Behandlung von Erkrankungen in niedriger Dosierung |
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