BR112013010855A2 - luciferases derivadas de oplophorus, novos substratos de coelenterazina e métodos de uso - Google Patents

luciferases derivadas de oplophorus, novos substratos de coelenterazina e métodos de uso

Info

Publication number
BR112013010855A2
BR112013010855A2 BR112013010855A BR112013010855A BR112013010855A2 BR 112013010855 A2 BR112013010855 A2 BR 112013010855A2 BR 112013010855 A BR112013010855 A BR 112013010855A BR 112013010855 A BR112013010855 A BR 112013010855A BR 112013010855 A2 BR112013010855 A2 BR 112013010855A2
Authority
BR
Brazil
Prior art keywords
oplophorus
methods
amino acid
substrates
novel coelenterazine
Prior art date
Application number
BR112013010855A
Other languages
English (en)
Portuguese (pt)
Inventor
Binkowski Brock
V Wood Keith
P Encell Lance
Hall Mary
B Robers Matthew
R Slater Michael
G Wood Monika
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44983728&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR112013010855(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Promega Corp filed Critical Promega Corp
Publication of BR112013010855A2 publication Critical patent/BR112013010855A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12005—Renilla-luciferin 2-monooxygenase (1.13.12.5), i.e. renilla-luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12013—Oplophorus-luciferin 2-monooxygenase (1.13.12.13)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
BR112013010855A 2010-11-02 2011-11-02 luciferases derivadas de oplophorus, novos substratos de coelenterazina e métodos de uso BR112013010855A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40942210P 2010-11-02 2010-11-02
PCT/US2011/059018 WO2012061530A2 (en) 2010-11-02 2011-11-02 Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use

Publications (1)

Publication Number Publication Date
BR112013010855A2 true BR112013010855A2 (pt) 2017-06-27

Family

ID=44983728

Family Applications (2)

Application Number Title Priority Date Filing Date
BR112013010855A BR112013010855A2 (pt) 2010-11-02 2011-11-02 luciferases derivadas de oplophorus, novos substratos de coelenterazina e métodos de uso
BR112013010487-2A BR112013010487B1 (pt) 2010-11-02 2011-11-02 compostos de coelenterazina, kit compreendendo ditos compostos e método para detectar luminescência em uma amostra in vitro

Family Applications After (1)

Application Number Title Priority Date Filing Date
BR112013010487-2A BR112013010487B1 (pt) 2010-11-02 2011-11-02 compostos de coelenterazina, kit compreendendo ditos compostos e método para detectar luminescência em uma amostra in vitro

Country Status (15)

Country Link
US (10) US8809529B2 (enExample)
EP (4) EP3498709B1 (enExample)
JP (6) JP5889910B2 (enExample)
KR (2) KR101538624B1 (enExample)
CN (3) CN103370319B (enExample)
AU (2) AU2011323418B2 (enExample)
BR (2) BR112013010855A2 (enExample)
CA (3) CA3081115A1 (enExample)
DK (2) DK2635582T3 (enExample)
ES (2) ES2567410T3 (enExample)
IL (5) IL225867B (enExample)
PL (2) PL2635595T4 (enExample)
PT (1) PT2635595T (enExample)
SG (5) SG189496A1 (enExample)
WO (2) WO2012061530A2 (enExample)

Families Citing this family (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2308978B8 (en) 2003-10-10 2015-04-08 Promega Corporation Luciferase biosensor
EP2327768B1 (en) 2006-04-03 2015-09-09 Promega Corporation Permuted and nonpermuted luciferase biosensors
US9045730B2 (en) 2008-05-19 2015-06-02 Promega Corporation Luciferase biosensors for cAMP
SG10201901453PA (en) * 2009-05-01 2019-03-28 Promega Corp Synthetic oplophorus luciferases with enhanced light output
US9290794B2 (en) * 2010-05-11 2016-03-22 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
JP5961163B2 (ja) 2010-05-11 2016-08-02 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 増強された検出特性を有する変異プロテアーゼバイオセンサー
BR112013010855A2 (pt) 2010-11-02 2017-06-27 Promega Corp luciferases derivadas de oplophorus, novos substratos de coelenterazina e métodos de uso
SG189494A1 (en) 2010-11-02 2013-05-31 Promega Corp Coelenterazine derivatives and methods of using same
JP6522339B2 (ja) 2011-11-21 2019-05-29 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ カルボキシｘローダミン類似体
US10067149B2 (en) 2012-12-12 2018-09-04 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
KR102272233B1 (ko) 2012-12-12 2021-07-01 프로메가 코포레이션 생체활성 제제의 세포 표적 포획을 위한 조성물 및 방법
CA2894435A1 (en) 2012-12-12 2014-06-19 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
JP6511720B2 (ja) 2013-02-28 2019-05-15 Jnc株式会社 コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法
US10168323B2 (en) 2013-03-15 2019-01-01 Promega Corporation Compositions and methods for capture of cellular targets of bioactive agents
KR102675860B1 (ko) * 2013-03-15 2024-06-20 프로메가 코포레이션 구조적 보완에 의한 바이오발광성의 활성화
WO2014151282A1 (en) 2013-03-15 2014-09-25 Promega Corporation Substrates for covalent tethering of proteins to functional groups or solid surfaces
CN105392892A (zh) * 2013-03-27 2016-03-09 六品科技公司 重组噬菌体和细菌检测方法
JP6040846B2 (ja) 2013-04-08 2016-12-07 Jnc株式会社 セレンテラジン類縁体
JP2016521996A (ja) * 2013-06-19 2016-07-28 サンプルシックス テクノロジーズ，インコーポレイティド ファージベースの細菌検出アッセイ
KR102445330B1 (ko) * 2013-07-12 2022-09-19 더 칠드런스 호스피탈 오브 필라델피아 Ａａｖ 벡터 및 항―ａａｖ （아데노-관련 바이러스） 중화 항체에 대한 검정
CN105555964B (zh) 2013-07-18 2020-04-17 洛桑联邦理工学院 生物样品中进行生物发光共振能量转移(bret)分析的方式和方法
JP6484987B2 (ja) 2013-10-21 2019-03-20 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
WO2015067302A1 (en) 2013-11-05 2015-05-14 Ecole Polytechnique Federale De Lausanne (Epfl) Sensor molecules and uses thereof
JP6442825B2 (ja) 2013-12-26 2018-12-26 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
EP3099683B1 (en) 2014-01-29 2020-08-05 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
EP3099691B1 (en) 2014-01-29 2019-11-20 Promega Corporation Pro-substrates for live cell applications
JP6256118B2 (ja) * 2014-03-11 2018-01-10 Jnc株式会社 エビルシフェラーゼの触媒蛋白質のキメラ遺伝子及びその使用法
JP6265020B2 (ja) * 2014-04-16 2018-01-24 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
KR102456212B1 (ko) 2014-05-23 2022-10-20 프로메가 코포레이션 발광성 기질을 안정화시키기 위한 조성물 및 방법
WO2016040788A1 (en) 2014-09-11 2016-03-17 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
US11365402B2 (en) * 2014-09-12 2022-06-21 Promega Corporation Internal protein tags
US9969991B2 (en) 2014-09-12 2018-05-15 Promega Corporation Internal protein tags
US20190054117A1 (en) * 2014-12-19 2019-02-21 Novartis Ag Dimerization switches and uses thereof
EP4455668A3 (en) 2015-02-05 2025-02-19 Promega Corporation Luciferase-based thermal shift assays
EP3304078B1 (en) 2015-06-05 2022-08-31 Promega Corporation Cell-permeable, cell-compatible, and cleavable linkers for covalent tethering of functional elements
EP3313846B1 (en) 2015-06-25 2020-05-06 Promega Corporation Thienopyrrole compounds and uses thereof as inhibitors of oplophorus-derived luciferases
US20170029798A1 (en) * 2015-07-27 2017-02-02 Cellivery Therapeutics, Inc. Development of Improved Cell-Permeable (iCP) Parkin Recombinant Protein as a Protein-Based Anti-Neurodegenerative Agent for the Treatment of Parkinson's Disease-Associated Phenotypes by Utilizing BBB-Penetrating Protein Delivery System MITT, Enabled by Advanced Macromolecule Transduction Domain (aMTD)
EP3416968B1 (en) 2016-02-15 2022-07-27 Promega Corporation Coelenterazine analogues
CN108779452B (zh) 2016-03-11 2022-05-03 国立研究开发法人产业技术综合研究所 发光酶蛋白
US10634680B2 (en) 2016-04-26 2020-04-28 University Of Utah Research Foundation Target-binding activated split reporter systems for analyte detection and related components and methods
CN105968114B (zh) * 2016-05-27 2018-11-09 山东大学 一种腔肠素类似物及其制备方法与应用
JP7212612B2 (ja) * 2016-07-28 2023-01-25 プロメガ コーポレイション セレンテラジン類縁体
US10202584B2 (en) * 2016-09-01 2019-02-12 The Regents Of The University Of California Red-shifted luciferase-luciferin pairs for enhanced bioluminescence
US10316070B2 (en) 2016-09-09 2019-06-11 Promega Corporation Dual protected pro-coelenterazine substrates
EP4144738A1 (en) 2016-11-01 2023-03-08 Promega Corporation Coelenterazine analogues tethered to energy acceptors
US10400264B2 (en) * 2016-12-01 2019-09-03 Promega Corporation 5,5-disubstituted luciferins and their use in luciferase-based assays
WO2018102693A1 (en) * 2016-12-01 2018-06-07 Promega Corporation Cell impermeable coelenterazine analogues
JP7161475B2 (ja) 2016-12-28 2022-10-26 プロメガ コーポレイション 官能化ｎａｎｏｌｕｃ阻害剤
WO2018219953A1 (en) 2017-05-30 2018-12-06 Ecole polytechnique fédérale de Lausanne (EPFL) Sensors, methods and kits for detecting nadph based on resonance energy transfer.
ES2983923T3 (es) 2017-07-06 2024-10-28 Promega Corp Análogos de la pro-coelenterazina estables en suero
CN108107209B (zh) * 2017-09-19 2020-07-24 中国中医科学院医学实验中心 一种检测分泌型报告蛋白含量的优化方法及其所用生物材料
US11655247B2 (en) 2018-05-01 2023-05-23 Promega Corporation Nanoluc suicide substrates
US11442063B2 (en) 2018-05-30 2022-09-13 Promega Corporation Broad spectrum kinase binding agents
EP3802514B1 (en) 2018-06-01 2022-08-03 Promega Corporation Inhibitors of oplophorus luciferase-derived bioluminescent complexes
CN112567028A (zh) * 2018-06-12 2021-03-26 普洛麦格公司 多部分萤光素酶
US10962538B2 (en) 2018-06-14 2021-03-30 The Trustees Of Columbia University In The City Of New York Assays using arrestin recruitment and unmodified receptors
WO2020023871A1 (en) 2018-07-27 2020-01-30 Promega Corporation Quinone-containing conjugates
EP3981839B1 (en) * 2018-08-23 2025-08-20 Promega Corporation Coelenterazine analogs for use in luciferase-based assays
KR20250029277A (ko) 2018-10-03 2025-03-04 프로메가 코포레이션 코엘렌테라진 및 이의 유사체 및 유도체를 안정화하기 위한 조성물 및 방법
US11175228B2 (en) 2018-11-28 2021-11-16 Promega Corporation Reactive peptide labeling
EP3891503A2 (en) 2018-12-04 2021-10-13 Promega Corporation Broad spectrum gpcr binding agents
WO2020176755A1 (en) * 2019-02-27 2020-09-03 University Of Virginia Patent Foundation Atp-independent bioluminescent reporter variants to improve in vivo imaging
JP7584433B2 (ja) 2019-03-20 2024-11-15 プロメガ コーポレイション 光親和性プローブ
US11360096B2 (en) 2019-05-16 2022-06-14 Duke University Complex BRET technique for measuring biological interactions
JP7650829B2 (ja) 2019-06-24 2025-03-25 プロメガ コーポレイション 細胞中に生体分子を送達するための修飾ポリアミンポリマー
JP7713448B2 (ja) 2019-11-27 2025-07-25 プロメガ コーポレイション 多分子ルシフェラーゼペプチド及びポリペプチド
US12061202B2 (en) 2019-12-10 2024-08-13 Promega Corporation Compositions and methods for bioluminescent detection using multifunctional probes
JP7790724B2 (ja) * 2020-03-17 2025-12-23 国立研究開発法人産業技術総合研究所 発光基質化合物
EP3882632A1 (en) 2020-03-18 2021-09-22 Universite De Bordeaux Novel ion conducting channel fusion subunits and methods of use thereof
CN113527231A (zh) * 2020-04-15 2021-10-22 深圳华大生命科学研究院 杂环取代腔肠素类化合物的合成方法
EP3904508A1 (en) * 2020-04-27 2021-11-03 Institut Pasteur Luciferase linked immunosorbent assay
JP7677997B2 (ja) 2020-05-22 2025-05-15 プロメガ コーポレイション キナーゼターゲットエンゲージメントの増強
EP4204808A1 (en) 2020-08-28 2023-07-05 Promega Corporation Target engagement assay for ras proteins
JPWO2022138892A1 (enExample) 2020-12-25 2022-06-30
CN118703457A (zh) * 2021-04-01 2024-09-27 深圳华大生命科学研究院 一种刺虾荧光素酶Nluc的突变体及其应用
US20240361321A1 (en) * 2021-08-24 2024-10-31 Institut Pasteur Luminescent based antigen assay
EP4519290A1 (en) 2022-05-04 2025-03-12 Promega Corporation Circularly permuted dehalogenase variants
WO2023215505A1 (en) 2022-05-04 2023-11-09 Promega Corporation Modified dehalogenase with extended surface loop regions
JP2025516354A (ja) 2022-05-04 2025-05-27 プロメガ コーポレイション スプリット修飾デハロゲナーゼバリアント
US20230357266A1 (en) 2022-05-04 2023-11-09 Promega Corporation Photoactivatable compounds and uses thereof
CN115349611B (zh) * 2022-08-05 2023-10-24 江南大学 一种改善南美白对虾虾糜凝胶品质的方法
CN116068198B (zh) * 2022-11-30 2024-01-09 深圳湾实验室 Ppi原位检测方法及其载体、诊断试剂、试剂盒和应用
US20240377404A1 (en) * 2023-03-24 2024-11-14 Promega Corporation Bioluminescence-triggered photocatalytic activation
JP7482563B1 (ja) 2023-06-21 2024-05-14 国立大学法人大阪大学 シイノトモシビタケ由来の発光関連遺伝子
DE102023120682B3 (de) 2023-08-03 2024-10-24 Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut Zytotoxizitätstest zum Nachweis von Zellschäden
GB202404021D0 (en) 2024-03-20 2024-05-01 Ipsen Biopharm Ltd Cell-based neurotoxin assay

Family Cites Families (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0134108B1 (en) 1983-08-17 1989-04-05 AlliedSignal Inc. Gas generator and method of generating gas
JPH0515398A (ja) 1991-07-15 1993-01-26 Nippon Oil & Fats Co Ltd オキシダーゼ酵素の定量方法
US5368484A (en) 1992-05-22 1994-11-29 Atari Games Corp. Vehicle simulator with realistic operating feedback
US6387675B1 (en) 1994-01-03 2002-05-14 Promega Corporation Mutant luciferases
AU698424C (en) 1994-01-03 2002-10-10 Promega Corporation Mutant luciferases
GB9501170D0 (en) 1994-03-23 1995-03-08 Secr Defence Luciferases
JP3648763B2 (ja) 1994-08-23 2005-05-18 日本油脂株式会社 ウミホタルルシフェリン誘導体および糖加水分解酵素の定量方法
GB9417593D0 (en) 1994-09-01 1994-10-19 Secr Defence Luciferase labelling method
US5670356A (en) 1994-12-12 1997-09-23 Promega Corporation Modified luciferase
US6171808B1 (en) 1995-01-20 2001-01-09 The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland Mutant luciferases
JPH08294397A (ja) 1995-04-27 1996-11-12 Nippon Oil & Fats Co Ltd 免疫学的活性物質の測定方法
US5998204A (en) 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
GB9707486D0 (en) 1997-04-11 1997-05-28 Secr Defence Enzyme assays
WO1998046739A1 (fr) 1997-04-16 1998-10-22 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Molecules immunomodulatrices et leur procede de production
US6602677B1 (en) 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
CA2301828A1 (en) 1997-09-19 1999-03-25 Promega Corporation Thermostable luciferases and methods of production
GB9823468D0 (en) 1998-10-28 1998-12-23 Secr Defence Novel enzyme
JP3694181B2 (ja) 1999-01-05 2005-09-14 キッコーマン株式会社 変異型ルシフェラーゼ、変異型ルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び変異型ルシフェラーゼの製造法
GB9925161D0 (en) 1999-10-26 1999-12-22 Secr Defence Novel enzyme
CN100516223C (zh) 1999-10-26 2009-07-22 3M创新有限公司 新型酶
US20010046687A1 (en) 2000-03-31 2001-11-29 Dicesare Joseph L. Method for the enhancement of luminescence intensity from a reaction between adenosine triphosphate (ATP) and a luciferase/luciferin reactant system
JP4613441B2 (ja) * 2000-04-26 2011-01-19 チッソ株式会社 新規ルシフェラーゼおよび発光蛋白質
DE60142761D1 (de) 2000-04-26 2010-09-23 Chisso Corp Oplophorus Luciferase
US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
US7268229B2 (en) 2001-11-02 2007-09-11 Promega Corporation Compounds to co-localize luminophores with luminescent proteins
US7148030B2 (en) 2002-02-01 2006-12-12 Promega Corporation Bioluminescent protease assay
CA2497560A1 (en) 2002-09-20 2004-04-01 Promega Corporation Luminescence-based methods and probes for measuring cytochrome p450 activity
ATE290016T1 (de) * 2002-10-21 2005-03-15 Axxam S R L Photoprotein mit verbesserter biolumineszenz
US7741067B2 (en) 2002-12-23 2010-06-22 Promega Corporation Luciferase-based assays
AU2004211584B2 (en) 2003-01-31 2009-11-12 Promega Corporation Covalent tethering of functional groups to proteins
US7429472B2 (en) 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
AU2004210982B2 (en) 2003-02-12 2008-12-18 Promega Corporation Methods and kits for dual enzymatic assays whereby light is quenched from luminescent reactions
US20040178545A1 (en) 2003-03-14 2004-09-16 Cates Larry E. System for optically analyzing a molten metal bath
EP2308978B8 (en) 2003-10-10 2015-04-08 Promega Corporation Luciferase biosensor
ES2334923T3 (es) 2004-01-08 2010-03-17 Universitat Zurich Complejos metalicos que utilizan la vitamina b12 como ligando.
US7416854B2 (en) 2004-01-22 2008-08-26 Promega Corporation Luminogenic and nonluminogenic multiplex assay
US7553632B2 (en) 2004-01-22 2009-06-30 Promega Corporation Luminogenic and nonluminogenic multiplex assay
US20070087400A1 (en) 2004-07-30 2007-04-19 Aldis Darzins Covalent tethering of functional groups to proteins and substrates therefor
EP3179252B1 (en) 2004-07-30 2018-11-14 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US7728118B2 (en) 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
JP4639904B2 (ja) 2005-03-30 2011-02-23 チッソ株式会社 蛍光活性を有するルシフェラーゼの活性増強方法
JP3844082B2 (ja) * 2005-04-08 2006-11-08 キッコーマン株式会社 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び耐熱性ホタルルシフェラーゼの製造法
EP2272973B1 (en) 2005-05-31 2015-05-27 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
EP1935986B1 (en) 2005-05-31 2014-04-16 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
US7495579B2 (en) 2005-06-13 2009-02-24 Sirota J Marcos Traffic light status remote sensor system
CA2616930A1 (en) 2005-08-17 2007-02-22 Yeda Research And Development Co. Ltd. Methods and compositions for determining a level of biologically active serum paraoxonase
JP4999341B2 (ja) 2005-09-06 2012-08-15 株式会社バイオエネックス 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法
EP2327768B1 (en) 2006-04-03 2015-09-09 Promega Corporation Permuted and nonpermuted luciferase biosensors
JP2007306871A (ja) 2006-05-19 2007-11-29 Chisso Corp 封入体可溶化方法及び精製方法
WO2008054821A2 (en) 2006-10-30 2008-05-08 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression
WO2008086035A2 (en) 2007-01-10 2008-07-17 Promega Corporation Split mutant hydrolase fusion reporter and uses thereof
US20080248511A1 (en) 2007-03-26 2008-10-09 Promega Corporation Methods to quench light from optical reactions
US20090253131A1 (en) 2007-11-05 2009-10-08 Promega Corporation Hybrid fusion reporter and uses thereof
US9045730B2 (en) 2008-05-19 2015-06-02 Promega Corporation Luciferase biosensors for cAMP
CN102159948B (zh) 2008-07-22 2014-06-25 普罗美加公司 基于adp检测的发光磷酸转移酶或atp水解酶测定
TWI375901B (en) 2008-08-05 2012-11-01 Elan Microelectronics Corp Touch screen and method for positioning coordinate
WO2010090319A1 (ja) * 2009-02-09 2010-08-12 チッソ株式会社 セレンテラジン類縁体及びその製造方法
WO2010119721A1 (ja) * 2009-04-17 2010-10-21 独立行政法人産業技術総合研究所 超高輝度で安定な人工生物発光酵素
SG10201901453PA (en) 2009-05-01 2019-03-28 Promega Corp Synthetic oplophorus luciferases with enhanced light output
WO2011038219A1 (en) 2009-09-24 2011-03-31 Promega Corporation Proteases having improved stability, solubility and activity
JP5605727B2 (ja) * 2010-04-06 2014-10-15 Ｊｎｃ株式会社 セレンテラジン類縁体及びセレンテラミド類縁体
JP5961163B2 (ja) 2010-05-11 2016-08-02 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 増強された検出特性を有する変異プロテアーゼバイオセンサー
WO2012030960A1 (en) 2010-09-01 2012-03-08 Promega Corporation Oxidized glutathione assay
US9284188B2 (en) 2010-09-03 2016-03-15 The Regents Of The University Of California Cytosolic delivery of materials with endosome-disrupting colloids
SG189494A1 (en) * 2010-11-02 2013-05-31 Promega Corp Coelenterazine derivatives and methods of using same
BR112013010855A2 (pt) 2010-11-02 2017-06-27 Promega Corp luciferases derivadas de oplophorus, novos substratos de coelenterazina e métodos de uso
US9273343B2 (en) 2011-09-02 2016-03-01 Promega Corporation Compounds and methods for assaying redox state of metabolically active cells and methods for measuring NAD(P)/NAD(P)H

Also Published As

Publication number Publication date
CA2815227A1 (en) 2012-05-10
US11661623B2 (en) 2023-05-30
US20140227759A1 (en) 2014-08-14
EP3498710A1 (en) 2019-06-19
ES2723773T3 (es) 2019-09-02
AU2011323418B2 (en) 2017-07-27
ES2567410T3 (es) 2016-04-22
KR20130099997A (ko) 2013-09-06
BR112013010487B1 (pt) 2021-02-02
US9840730B2 (en) 2017-12-12
IL226114B (en) 2018-04-30
EP3498709A1 (en) 2019-06-19
PL2635595T3 (pl) 2019-04-30
AU2016213854B2 (en) 2018-01-04
EP3498709B1 (en) 2025-12-31
IL288070A (en) 2022-01-01
US20200399676A1 (en) 2020-12-24
CA2817102A1 (en) 2012-05-10
PL2635582T3 (pl) 2016-08-31
US20240158831A1 (en) 2024-05-16
CN106148362B (zh) 2021-04-30
CN103443121B (zh) 2016-04-20
US20220348983A1 (en) 2022-11-03
US12215375B2 (en) 2025-02-04
WO2012061529A1 (en) 2012-05-10
AU2016213854A1 (en) 2016-09-01
IL225867B (en) 2018-07-31
JP6539689B2 (ja) 2019-07-03
CN103370319B (zh) 2016-08-24
SG10201607774RA (en) 2016-11-29
IL274316B (en) 2021-12-01
DK2635582T3 (da) 2016-03-14
AU2011323418A1 (en) 2013-05-30
WO2012061530A3 (en) 2012-09-27
JP2017176182A (ja) 2017-10-05
CN103370319A (zh) 2013-10-23
JP6155424B2 (ja) 2017-07-05
KR101538624B1 (ko) 2015-07-22
KR101939553B1 (ko) 2019-01-17
IL258790A (en) 2018-06-28
CN103443121A (zh) 2013-12-11
JP6752839B2 (ja) 2020-09-09
WO2012061530A8 (en) 2013-06-06
BR112013010487A2 (pt) 2016-11-22
US8809529B2 (en) 2014-08-19
SG190104A1 (en) 2013-06-28
US10774364B2 (en) 2020-09-15
JP2014503485A (ja) 2014-02-13
KR20130084312A (ko) 2013-07-24
PL2635595T4 (pl) 2019-04-30
EP2635582B1 (en) 2016-01-27
JP6574721B2 (ja) 2019-09-11
JP2014500011A (ja) 2014-01-09
DK2635595T3 (en) 2019-03-04
SG10202103336SA (en) 2021-04-29
CA3081115A1 (en) 2012-05-10
SG10201608550SA (en) 2016-12-29
AU2011323419A1 (en) 2013-05-30
US20150064731A1 (en) 2015-03-05
CN106148362A (zh) 2016-11-23
JP6374420B2 (ja) 2018-08-15
US9951373B2 (en) 2018-04-24
US20180327810A1 (en) 2018-11-15
IL274316A (en) 2020-06-30
IL226114A0 (en) 2013-06-27
CA2817102C (en) 2020-07-28
EP2635595B1 (en) 2019-01-09
US20120117667A1 (en) 2012-05-10
US20140223590A1 (en) 2014-08-07
CA2815227C (en) 2019-03-26
IL288070B (en) 2022-10-01
JP2016144454A (ja) 2016-08-12
JP2016145219A (ja) 2016-08-12
IL288070B2 (en) 2023-02-01
JP2018127459A (ja) 2018-08-16
EP2635582A1 (en) 2013-09-11
PT2635595T (pt) 2019-01-29
US9139836B2 (en) 2015-09-22
IL258790B (en) 2020-05-31
US9938564B2 (en) 2018-04-10
JP5889910B2 (ja) 2016-03-22
US20160002703A1 (en) 2016-01-07
US8669103B2 (en) 2014-03-11
SG189496A1 (en) 2013-05-31
WO2012061530A2 (en) 2012-05-10
US20120174242A1 (en) 2012-07-05
IL225867A0 (en) 2013-07-31
US12435354B2 (en) 2025-10-07
EP2635595A2 (en) 2013-09-11

Similar Documents

Publication Publication Date Title
BR112013010855A2 (pt) luciferases derivadas de oplophorus, novos substratos de coelenterazina e métodos de uso
SG10201901453PA (en) Synthetic oplophorus luciferases with enhanced light output
BRPI0922510B8 (pt) penicilina g acilase procariótica mutante
CA3081446A1 (en) Methods and compositions for polypeptide analysis
CL2012003077A1 (es) Polipeptido que comprende el decimo dominio de fibronectina tipo iii (10fn3), en donde el dominio 10fn3 se une al dominio 1 o 2 de seroalbumina humana, y donde la vida media del suero del polipéptido en presencia de seroalbumina es al menos 5 veces mayor que en su ausencia; acido nucleico que lo codifica; vector que lo comprende; celula hospedera que lo expresa; composición que lo comprende; y su uso para tratar la diabetes.
BR112017008108A2 (pt) incorporação de aminoácidos não naturais em proteínas
CL2011002768A1 (es) Polipéptido mutante fgf21; ácido nucleico que lo codifica; vector; célula huésped; composición farmacéutica que lo comprende; y su uso para tratar un trastorno metabólico.
BR112012017345A2 (pt) "composições e métodos de pgl para a produção aumentada de isopreno e outros produtos com 6-fosfogliconolactonase (pgl)"
BR112017028205A2 (pt) molécula de rna, vetor, célula hospedeira, composição farmacêutica, kit, e, uso de uma ou mais utrs.
EA201001883A1 (ru) Мутанты fgf21 и их применение
NZ606584A (en) Binding proteins for hepcidin
BR112014026745A2 (pt) atualizações de conjunto de parâmetros em codificação de vídeo
ES2691070T3 (es) Péptidos antiinflamatorios y composición que comprende los mismos
PA8830501A1 (es) Co-agonistas del receptor de glucagon/glp-1
BR112013009157A2 (pt) polipeptídeos com atividade de permease
MX2017000083A (es) Compuestos de platino, composiciones y usos de estos.
BR112015005384A2 (pt) neurotoxinas clostridium botuliem recombinantes
MX371403B (es) Moleculas de andamiaje a base de fibronectina estabilizada.
EA200970981A1 (ru) Пептидная смесь в качестве стабилизатора вина
AR075933A1 (es) Composiciones polipeptidas y de inmunizacion conteniendo polipeptidos positivos gram y metodos de uso
MX2016006277A (es) Secuencia de endolisina kz144 modificada.
BR112015017797A2 (pt) celobio-hidrolase termoestável
BR112016025898A2 (pt) ?drimenol sintases e método para produzir drimenol?
EA201691011A1 (ru) Последовательность модифицированного эндолизина el188
BR112012011598A2 (pt) polipeptídeos de haemophilus parasuis e métodos de uso

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NAO 10196/2001, QUE MODIFICOU A LEI NAO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUAANCIA PRA VIA DA ANVISA. CONSIDERANDO A APROVAA AO DOS TERMOS DO PARECER NAO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NAO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDAANCIAS CABA-VEIS.

B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]