AU2013300171B2 - Methods and compositions for preparing biological specimens for microscopic analysis - Google Patents

Methods and compositions for preparing biological specimens for microscopic analysis Download PDF

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Publication number
AU2013300171B2
AU2013300171B2 AU2013300171A AU2013300171A AU2013300171B2 AU 2013300171 B2 AU2013300171 B2 AU 2013300171B2 AU 2013300171 A AU2013300171 A AU 2013300171A AU 2013300171 A AU2013300171 A AU 2013300171A AU 2013300171 B2 AU2013300171 B2 AU 2013300171B2
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specimen
hydrogel
tissue
embedded
microscopy
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AU2013300171A1 (en
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Kwanghun Chung
Karl A. DEISSEROTH
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Leland Stanford Junior University
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Leland Stanford Junior University
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44743Introducing samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44747Composition of gel or of carrier mixture
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/549Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic with antigen or antibody entrapped within the carrier
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/34Microscope slides, e.g. mounting specimens on microscope slides
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • G01N2001/4038Concentrating samples electric methods, e.g. electromigration, electrophoresis, ionisation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/4833Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Optics & Photonics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Microscoopes, Condenser (AREA)
AU2013300171A 2012-08-09 2013-03-13 Methods and compositions for preparing biological specimens for microscopic analysis Ceased AU2013300171B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261681551P 2012-08-09 2012-08-09
US61/681,551 2012-08-09
PCT/US2013/031066 WO2014025392A1 (en) 2012-08-09 2013-03-13 Methods and compositions for preparing biological specimens for microscopic analysis

Publications (2)

Publication Number Publication Date
AU2013300171A1 AU2013300171A1 (en) 2014-11-06
AU2013300171B2 true AU2013300171B2 (en) 2017-09-14

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AU2013300171A Ceased AU2013300171B2 (en) 2012-08-09 2013-03-13 Methods and compositions for preparing biological specimens for microscopic analysis

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US (3) US10545075B2 (enExample)
EP (2) EP4163617A1 (enExample)
JP (1) JP6228205B2 (enExample)
KR (1) KR102148747B1 (enExample)
CN (1) CN104350372B (enExample)
AU (1) AU2013300171B2 (enExample)
IN (1) IN2014DN09073A (enExample)
WO (1) WO2014025392A1 (enExample)

Families Citing this family (173)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP3604555B1 (en) 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequencing by structure assembly
WO2014163886A1 (en) 2013-03-12 2014-10-09 President And Fellows Of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
EP2794928B1 (en) 2011-12-22 2019-02-20 President and Fellows of Harvard College Compositions and methods for analyte detection
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
IN2014DN09073A (enExample) 2012-08-09 2015-05-22 Univ Leland Stanford Junior
WO2014060483A1 (en) 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
DE102013205115A1 (de) * 2013-03-22 2014-09-25 Leica Microsystems Cms Gmbh SPIM-Anordnung
US9868979B2 (en) 2013-06-25 2018-01-16 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
DE102013216934A1 (de) * 2013-08-26 2015-02-26 Leica Microsystems Cms Gmbh Verfahren und Vorrichtung zur Erhöhung der optischen Transparenz von Bereichen einer Gewebeprobe
US10794802B2 (en) * 2013-09-20 2020-10-06 California Institute Of Technology Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high resolution intact circuit mapping and phenotyping
WO2015041755A1 (en) * 2013-09-20 2015-03-26 California Institute Of Technology Methods for phenotyping of intact whole tissues
CN103558055B (zh) * 2013-09-29 2016-01-06 中国疾病预防控制中心病毒病预防控制所 一种针对感染病毒的病变细胞的定位超薄切片方法
WO2015127183A2 (en) * 2014-02-21 2015-08-27 Massachusetts Institute Of Technology Expansion microscopy
US10416116B2 (en) 2014-04-04 2019-09-17 Massachusetts Institute Of Technology Active transport of charged molecules into, within, and/or from charged matrices
JP2017517761A (ja) * 2014-05-30 2017-06-29 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 大きなインタクトな組織試料を撮像するための方法及び装置
JP6778186B2 (ja) * 2014-07-07 2020-10-28 ロゴス バイオシステムズ, インコーポレイテッド 電気泳動を使用する組織クリアリング装置
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
ES2968004T3 (es) 2014-07-30 2024-05-06 Harvard College Construcción de bibliotecas de sondas
WO2016023009A1 (en) * 2014-08-07 2016-02-11 Wake Forest University Health Sciences Compositions and methods for clearing a biological sample
KR101563826B1 (ko) * 2014-12-31 2015-10-28 계명대학교 산학협력단 조직 투명화 방법
EP3885762A1 (en) 2015-02-09 2021-09-29 Ventana Medical Systems, Inc. Methods for standardizing diffusion of a fluid into tissues
EP4282977B1 (en) 2015-04-10 2024-06-05 10x Genomics Sweden AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US10059990B2 (en) 2015-04-14 2018-08-28 Massachusetts Institute Of Technology In situ nucleic acid sequencing of expanded biological samples
US10526649B2 (en) 2015-04-14 2020-01-07 Massachusetts Institute Of Technology Augmenting in situ nucleic acid sequencing of expanded biological samples with in vitro sequence information
US11408890B2 (en) 2015-04-14 2022-08-09 Massachusetts Institute Of Technology Iterative expansion microscopy
CN106198990A (zh) * 2015-04-30 2016-12-07 上海交通大学 一种对组织样本进行免疫标记的方法
CN104965016A (zh) * 2015-06-15 2015-10-07 南昌大学 一体化的生物组织透明装置
US10317321B2 (en) 2015-08-07 2019-06-11 Massachusetts Institute Of Technology Protein retention expansion microscopy
WO2017027367A1 (en) * 2015-08-07 2017-02-16 Massachusetts Institute Of Technology Nanoscale imaging of proteins and nucleic acids via expansion microscopy
WO2017031249A1 (en) * 2015-08-17 2017-02-23 California Institute Of Technology Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high resolution intact circuit mapping and phenotyping
WO2017075265A1 (en) * 2015-10-28 2017-05-04 The Broad Institute, Inc. Multiplex analysis of single cell constituents
EP3371329B1 (en) 2015-11-03 2026-03-25 President and Fellows of Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
US20190113423A1 (en) * 2015-12-02 2019-04-18 Clearlight Diagnostics, LLC Methods for preparing and analyzing tumor tissue samples for detection and monitoring of cancers
CN108431664B (zh) * 2015-12-23 2021-11-02 皇家飞利浦有限公司 荧光校准载片
RU2746895C2 (ru) 2015-12-24 2021-04-21 Конинклейке Филипс Н.В. Устройство для окрашивания трехмерной биопсийной ткани
CN105823807B (zh) * 2016-01-04 2018-04-20 浙江农林大学 抗癌药物羟喜树碱药效检测装置及检测方法
US11254974B2 (en) 2016-02-10 2022-02-22 The Board Of Trustees Of The Leland Stanford Junior University RNA fixation and detection in clarity-based hydrogel tissue
EP3974387B1 (en) 2016-03-07 2025-10-29 X-Zell Inc. Compositions and methods for identifying rare cells
JP6721899B2 (ja) * 2016-03-30 2020-07-15 国立大学法人 大分大学 高親水性高分子モノマーを主剤とする電子顕微鏡用包埋方法
CA3210120C (en) 2016-04-25 2024-04-09 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
US11397140B2 (en) * 2016-04-29 2022-07-26 Massachusetts Institute Of Technology Methods for reversible and tunable tissue magnification
US10324022B2 (en) 2016-05-11 2019-06-18 Bonraybio Co., Ltd. Analysis accuracy improvement in automated testing apparatus
US10281386B2 (en) * 2016-05-11 2019-05-07 Bonraybio Co., Ltd. Automated testing apparatus
US10852290B2 (en) 2016-05-11 2020-12-01 Bonraybio Co., Ltd. Analysis accuracy improvement in automated testing apparatus
EP3252452A1 (en) * 2016-05-25 2017-12-06 The Board of Trustees of the Leland Stanford Junior University Method for imaging and analysis of a biological specimen
WO2017205531A1 (en) * 2016-05-25 2017-11-30 The Board Of Trustees Of The Leland Stanford Junior University Methods for visualization and quantification of fiber-like structures
WO2017222453A1 (en) 2016-06-21 2017-12-28 Hauling Thomas Nucleic acid sequencing
CN107621462B (zh) * 2016-07-13 2020-03-31 王志伟 一种组织透明化液sut及其制备和应用
CN107674879A (zh) * 2016-08-01 2018-02-09 深圳先进技术研究院 一种光‑基因质粒及其应用
US11480502B2 (en) 2016-08-29 2022-10-25 United States of America, as represented by the Secretary, Department of Heatlth and Human Service Method and composition for optical clearing of tissues
JP7239465B2 (ja) 2016-08-31 2023-03-14 プレジデント アンド フェローズ オブ ハーバード カレッジ 蛍光in situ配列決定による検出のための核酸配列ライブラリの作製法
GB2569252A (en) 2016-08-31 2019-06-12 Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
US10538632B1 (en) 2016-09-30 2020-01-21 National Technology & Engineering Solutions Of Sandia, Llc Shape-preserving polymeric replication of biological matter
JP6898720B2 (ja) * 2016-10-14 2021-07-07 株式会社ファンケル 皮膚内部構造評価方法
EP3539036A4 (en) * 2016-11-08 2020-06-17 President and Fellows of Harvard College MATRIX AND DELETE
US20190276881A1 (en) * 2016-11-08 2019-09-12 President And Fellows Of Harvard College Multiplexed imaging using merfish, expansion microscopy, and related technologies
KR101866249B1 (ko) 2016-11-29 2018-06-12 박순현 생체 조직 투명화용 조성물 및 이를 이용한 생체 조직 투명화 방법
DE102016123458B3 (de) * 2016-12-05 2018-03-15 Georg-August-Universität Göttingen Stiftung Öffentlichen Rechts, Universitätsmedizin Verfahren zur Herstellung transparenter biologischer Präparate für eine lichtmikroskopische Untersuchung
CN106682453B (zh) * 2016-12-28 2019-03-26 长春理工大学 一种dna分子的定点编辑方法
US10955322B2 (en) 2017-01-18 2021-03-23 California Institute Of Technology Methods and devices for soft and osseous tissue clearing and fluorescent imaging
CN106769350B (zh) 2017-01-20 2019-11-22 上海交通大学 一种用于富含脂滴组织快速完全透明化的方法
US10995361B2 (en) * 2017-01-23 2021-05-04 Massachusetts Institute Of Technology Multiplexed signal amplified FISH via splinted ligation amplification and sequencing
KR101888782B1 (ko) * 2017-02-07 2018-08-14 고려대학교 산학협력단 피부 조직의 투명화 방법, 이를 이용한 피부 조직의 3차원 영상화 방법 및 평가 및 진단 방법
US11436719B2 (en) 2017-02-16 2022-09-06 Kyoto University Cell evaluation method, cell evaluation device, and recording medium storing cell evaluation program
WO2018157048A1 (en) 2017-02-24 2018-08-30 Massachusetts Institute Of Technology Methods for examining podocyte foot processes in human renal samples using conventional optical microscopy
WO2018157074A1 (en) 2017-02-24 2018-08-30 Massachusetts Institute Of Technology Methods for diagnosing neoplastic lesions
WO2018155925A2 (ko) * 2017-02-27 2018-08-30 고려대학교 산학협력단 하이드로겔 중합체를 이용한 탈세포화 조직의 제조방법 및 이로부터 제조된 탈세포화 조직
KR20180099482A (ko) * 2017-02-27 2018-09-05 고려대학교 산학협력단 하이드로겔 중합체를 이용한 탈세포화 조직의 제조방법 및 이로부터 제조된 탈세포화 조직
TWI620927B (zh) * 2017-04-21 2018-04-11 胡宇光 神經組織樣本染色之方法及套組與顯現神經元之方法
WO2018218150A1 (en) 2017-05-26 2018-11-29 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
MX2017008919A (es) * 2017-07-05 2018-03-01 Gabriela Reyes Fuchs Carmen Proceso para formar una imagen a color de materiales incinerados mediante tecnicas de microscopia.
JP7160350B2 (ja) * 2017-07-06 2022-10-25 公立大学法人大阪 生体組織透明化法及びその試薬
KR101996722B1 (ko) * 2017-07-07 2019-07-04 재단법인대구경북과학기술원 다채널 박막전극을 이용한 전기적 생체분자 이동방법
US11180804B2 (en) 2017-07-25 2021-11-23 Massachusetts Institute Of Technology In situ ATAC sequencing
EP4589017A3 (en) 2017-10-06 2025-10-08 10x Genomics, Inc. Rna templated ligation
KR102200618B1 (ko) * 2018-01-12 2021-01-08 재단법인대구경북과학기술원 설포베타인계 쯔비터이온성 계면활성제를 포함하는 생물학적 분자의 조직침투용 조성물 및 이의 용도
WO2019078655A1 (ko) * 2017-10-19 2019-04-25 재단법인대구경북과학기술원 설포베타인계 쯔비터이온성 계면활성제를 포함하는 생물학적 분자의 조직침투용 조성물 및 이의 용도
US10488307B2 (en) * 2017-12-12 2019-11-26 Dignity Health Systems, devices, and methods for improved tissue treatment
KR102050248B1 (ko) * 2017-12-29 2019-11-29 연세대학교 산학협력단 조직 투명도 분석 방법
CN107966477A (zh) * 2018-01-22 2018-04-27 哈尔滨工业大学 一种生物敏感元件
US11873374B2 (en) 2018-02-06 2024-01-16 Massachusetts Institute Of Technology Swellable and structurally homogenous hydrogels and methods of use thereof
GB2571544B (en) 2018-02-28 2022-05-18 Bright Instrument Co Ltd Sectioning macrotome
CN112188899B (zh) * 2018-03-16 2025-02-07 克里约凯珀赛尔公司 通过光学和/或电子显微技术成像的生物样品的包埋剂
WO2020011735A2 (en) 2018-07-09 2020-01-16 Universität Zürich Systems, apparatuses, and methods for preparation of tissue samples
WO2020013833A1 (en) 2018-07-13 2020-01-16 Massachusetts Institute Of Technology Dimethylacrylamide (dmaa) hydrogel for expansion microscopy (exm)
WO2020028194A1 (en) 2018-07-30 2020-02-06 Readcoor, Inc. Methods and systems for sample processing or analysis
KR102151128B1 (ko) * 2018-08-24 2020-09-02 주식회사 바이나리 질소 가스 가압식 조직 투명화 및 항체 침투 증진용 장치
JP7246752B2 (ja) * 2018-08-29 2023-03-28 国立研究開発法人物質・材料研究機構 測定装置、及び、評価方法
KR102368585B1 (ko) * 2018-09-28 2022-03-03 서울대학교산학협력단 온도에 따라 가역적 팽창/초기화가 가능한 하이드로젤을 이용한 생체조직 이미징
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
US11435302B2 (en) * 2018-11-13 2022-09-06 The Trustees Of Princeton University X-ray assisted electron microscopy staining procedure
GB201818567D0 (en) * 2018-11-14 2018-12-26 Francis Crick Institute Ltd Composition
CN113366116A (zh) 2018-12-13 2021-09-07 哈佛学院院长及董事 用于merfish和其他应用的扩增方法和系统
CN109612811A (zh) * 2018-12-26 2019-04-12 华中科技大学苏州脑空间信息研究院 一种保护生物组织结构和荧光的水凝胶包埋方法
CN109781493A (zh) * 2018-12-26 2019-05-21 华中科技大学苏州脑空间信息研究院 一种可长时间连续精细切削成像的生物组织水凝胶包埋方法
WO2020172247A1 (en) 2019-02-22 2020-08-27 Massachusetts Institute Of Technology Iterative direct expansion microscopy
CN110006862B (zh) * 2019-03-29 2020-09-08 华中科技大学 膨胀切削显微成像方法及适用于该方法的超吸水水凝胶
CN112740013A (zh) * 2019-05-09 2021-04-30 科来旺科技公司 使用离子导电膜对生物样本进行电泳染色的方法和设备
CN114174799A (zh) * 2019-05-30 2022-03-11 贝克曼库尔特有限公司 用于固定生物标本以进行显微成像的方法和系统
AU2020282024A1 (en) 2019-05-31 2021-11-11 10X Genomics, Inc. Method of detecting target nucleic acid molecules
US20220276139A1 (en) * 2019-07-08 2022-09-01 The University Of Hong Kong Compositions and methods for clearing tissue
US12265004B2 (en) 2019-11-05 2025-04-01 Massachusetts Institute Of Technology Membrane probes for expansion microscopy
JPWO2021095718A1 (enExample) * 2019-11-11 2021-05-20
US11802822B2 (en) 2019-12-05 2023-10-31 Massachusetts Institute Of Technology Multiplexed expansion (MultiExM) pathology
GB201919032D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
WO2021155063A1 (en) 2020-01-29 2021-08-05 Readcoor, Llc Compositions and methods for analyte detection
US12110548B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis
US12215379B2 (en) 2020-02-17 2025-02-04 10X Genomics, Inc. In situ analysis of chromatin interaction
JP2023514749A (ja) 2020-02-21 2023-04-07 10エックス ジェノミクス インコーポレイテッド 統合型インサイチュ空間的アッセイのための方法および組成物
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
EP4117820B1 (en) * 2020-03-13 2026-02-25 Yale University Methods for physical expansion and imaging of biological samples
WO2021226198A1 (en) * 2020-05-05 2021-11-11 Massachusetts Institute Of Technology Compositions and methods relating to reversibly compressible tissue-hydrogel hybrids
CN111397999B (zh) * 2020-05-09 2021-12-03 中国科学院植物研究所 一种增强显微ct植物样品对比度的简易方法
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
US12297499B2 (en) 2020-08-17 2025-05-13 10X Genomics, Inc. Multicomponent nucleic acid probes for sample analysis
CN114129780B (zh) * 2020-09-03 2022-06-28 天津大学 一种光控释放一氧化氮的复合水凝胶的制备及应用
RU2752751C1 (ru) * 2020-09-28 2021-08-02 Ахмеджан Атаханович Аллаберганов Способ получения цифровых двойников документа в виде бумажного носителя текстовой информации или в виде изображения текстового документа
US20220121900A1 (en) * 2020-10-19 2022-04-21 Arizona Board Of Regents On Behalf Of Northern Arizona University Methods and systems for generating unclonable optical tags
CN112284861B (zh) * 2020-10-21 2023-02-17 上海市农业科学院 用于真姬菇担孢子显微观察的固定液、制备方法、固定方法及应用
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
US11333588B1 (en) * 2020-12-07 2022-05-17 Nebulum Technologies Co., Ltd. Matrix-assisted methods and compositions to prepare biological samples for super-resolution imaging
EP4012046A1 (en) 2020-12-11 2022-06-15 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
CN112665951B (zh) * 2020-12-22 2022-11-11 中国农业科学院作物科学研究所 一种胚乳组织包埋方法与应用
WO2022140570A1 (en) 2020-12-23 2022-06-30 10X Genomics, Inc. Methods and compositions for analyte detection
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
EP4284945B1 (en) 2021-01-26 2024-10-23 10X Genomics, Inc. Nucleic acid analog probes for in situ analysis
KR102762387B1 (ko) * 2021-02-24 2025-02-05 한국과학기술원 화학적 염색과 팽창 이미징 기법의 최적화 방법
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
WO2022187366A1 (en) 2021-03-03 2022-09-09 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
CN113004885B (zh) * 2021-03-08 2024-06-11 光华临港工程应用技术研发(上海)有限公司 一种用于膨胀超分辨成像的dna纳米结构染料及其应用
WO2022256324A1 (en) 2021-06-01 2022-12-08 10X Genomics, Inc. Methods and compositions for analyte detection and probe resolution
WO2022256422A1 (en) 2021-06-02 2022-12-08 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
US20230012607A1 (en) 2021-07-09 2023-01-19 10X Genomics, Inc. Methods for detecting analytes using sparse labelling
CN117651855A (zh) 2021-07-13 2024-03-05 10X基因组学有限公司 用于制备具有可控厚度的聚合基质的方法
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
ES2988400T3 (es) 2021-07-30 2024-11-20 10X Genomics Inc Métodos y composiciones para sincronizar reacciones in situ
WO2023015192A1 (en) 2021-08-03 2023-02-09 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
US12529096B2 (en) 2021-08-03 2026-01-20 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid structures
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
EP4446426B1 (en) 2021-08-16 2026-03-04 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
US20230242974A1 (en) 2021-12-27 2023-08-03 10X Genomics, Inc. Methods and compositions for rolling circle amplification
EP4466376B1 (en) 2022-01-21 2026-03-04 10X Genomics, Inc. Multiple readout signals for analyzing a sample
EP4490737A1 (en) 2022-03-08 2025-01-15 10X Genomics, Inc. In situ code design methods for minimizing optical crowding
CN119032271A (zh) 2022-04-01 2024-11-26 10X基因组学有限公司 用于靶向掩蔽自发荧光的组合物和方法
WO2023196526A1 (en) 2022-04-06 2023-10-12 10X Genomics, Inc. Methods for multiplex cell analysis
WO2023204581A1 (ko) * 2022-04-20 2023-10-26 동국대학교 산학협력단 암세포 사멸효과를 가지는 3d 하이드로젤 복합체 및 이의 제조방법
WO2023215603A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Methods and compositions for in situ analysis of v(d)j sequences
WO2023215612A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
US20240084378A1 (en) 2022-05-11 2024-03-14 10X Genomics, Inc. Compositions and methods for in situ sequencing
EP4540607A1 (en) 2022-06-17 2025-04-23 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
KR102582110B1 (ko) * 2022-07-18 2023-09-21 (재)씨젠의료재단 3차원 세포 또는 생체 조직의 투명화 및 이미지 분석용 조성물
CN119677873A (zh) 2022-08-16 2025-03-21 10X基因组学有限公司 Ap50聚合酶及其用途
KR20240042887A (ko) * 2022-09-26 2024-04-02 연세대학교 산학협력단 고배율의 세포 확대 분석을 위한 세포 필름, 이의 제조용 조성물 및 제조 방법
CN115656120B (zh) * 2022-10-09 2024-07-23 中国科学院深圳先进技术研究院 一种暗场反射紫外光学显微成像方法和系统
WO2024081869A1 (en) 2022-10-14 2024-04-18 10X Genomics, Inc. Methods for analysis of biological samples
EP4573210A1 (en) 2022-11-16 2025-06-25 10X Genomics, Inc. Methods and compositions for assessing performance of in situ assays
EP4634404A1 (en) 2022-12-16 2025-10-22 10X Genomics, Inc. Methods and compositions for assessing performance
EP4638789A1 (en) 2022-12-21 2025-10-29 10X Genomics, Inc. Methods for tethering ribonucleic acids in biological samples
CN121014083A (zh) * 2023-03-31 2025-11-25 富士胶片株式会社 药物研发辅助装置、药物研发辅助装置的工作方法、及药物研发辅助装置的工作程序
US20240376521A1 (en) 2023-05-10 2024-11-14 10X Genomics, Inc. Boronic acid compositions and methods for tethering ribonucleic acids in biological samples
TWI887661B (zh) * 2023-05-25 2025-06-21 國立清華大學 用於將生物組織透明化的電極組及裝置
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification
US20250163502A1 (en) 2023-11-22 2025-05-22 10X Genomics, Inc. Methods for processing ribonucleic acids in biological samples
US20250207203A1 (en) 2023-12-20 2025-06-26 10X Genomics, Inc. In situ detection of copy number variations in biological samples
WO2025255457A1 (en) 2024-06-07 2025-12-11 10X Genomics, Inc. Compositions and methods for improved on-target spatial profiling
WO2025264702A1 (en) 2024-06-18 2025-12-26 10X Genomics, Inc. 3d hydrogels for nucleic acid sequencing
WO2026043885A1 (en) 2024-08-20 2026-02-26 10X Genomics, Inc. Methods and systems for preparing a nucleic acid library
WO2026055487A1 (en) 2024-09-06 2026-03-12 10X Genomics, Inc. Modified nucleotides with oligomer spacers

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070134798A1 (en) * 2005-12-08 2007-06-14 Mccormick Patric Light curing fixative

Family Cites Families (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3129158A (en) 1961-01-24 1964-04-14 Raymond Samuel Process for gel electrophoresis
US3208929A (en) 1962-09-11 1965-09-28 Robert H Raymond Apparatus for gel electrophoresis
US3346479A (en) 1964-04-09 1967-10-10 Scientific Industries Preparative separation by a combination of gel separation and electrophoresis
US3375187A (en) 1965-05-13 1968-03-26 Buchler Instr Inc Apparatus for temperature controlled preparative and analytical electrophoresis
US3576727A (en) 1968-05-23 1971-04-27 Bruce Lee Evatt Gel electrophoresis process
US3563880A (en) 1968-08-28 1971-02-16 Arthur W Anderson Gel electrophoresis unit
SE328135B (enExample) 1968-09-19 1970-09-07 Lkb Produkter Ab
US3616454A (en) 1969-03-13 1971-10-26 Univ New York Method of and apparatus for electrophoretic separation in a gel column
US3674678A (en) 1970-10-28 1972-07-04 Millipore Corp Electrophoretic apparatus
US3865712A (en) 1972-04-13 1975-02-11 Lkb Produkter Ab Electrode arrangement for an electrophoretical separation performed in a gel plate
US3989613A (en) * 1973-05-16 1976-11-02 The Dow Chemical Company Continuous balanced flow fixed boundary electrophoresis
US4088561A (en) 1977-06-27 1978-05-09 The United States Of America As Represented By The United States Department Of Energy Apparatus for electrophoresis separation
US4151065A (en) 1978-01-30 1979-04-24 The Regents Of The University Of California Horizontal slab gel electrophoresis
US4292161A (en) 1979-01-03 1981-09-29 Hoefer Scientific Instruments Vertical gel slab electrophoresis apparatus
FR2496889A1 (fr) 1980-12-22 1982-06-25 Hours Michel Cuve de migration et de reaction utilisable pour des analyses par electrophorese
US4339327A (en) 1981-04-23 1982-07-13 Tyler Jonathan M Miniature slab gel electrophoresis system
US4375401A (en) 1981-05-20 1983-03-01 Nicholas Catsimpoolas Electrophoresis system
EP0101859B1 (de) 1982-07-23 1987-10-14 Matthias Hediger Einrichtung für präparative Gelelektrophorese, insbesondere zur Gemischtrennung an einer einen Gradienten enthaltenden Polyacrylamid-Gelsäure
US4588491A (en) 1984-02-27 1986-05-13 International Biotechnologies, Inc. Horizontal gel electrophoresis device
EP0171676B1 (en) * 1984-07-31 1990-11-07 Hitachi, Ltd. Free-flow electrophoretic separation method and apparatus therefor
US4685025A (en) 1985-03-14 1987-08-04 Raychem Corporation Conductive polymer circuit protection devices having improved electrodes
US5188963A (en) 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
JPH03167472A (ja) * 1989-11-27 1991-07-19 Sumitomo Chem Co Ltd 実験動物の胎児骨格染色法
JP3216907B2 (ja) 1992-05-18 2001-10-09 株式会社日立製作所 電子ズーム装置及び撮像装置
JP3049537B2 (ja) * 1995-02-03 2000-06-05 悌二 竹崎 生検試料の定着支持方法とその定着支持剤及び包埋カセット
US6722395B2 (en) 1998-01-13 2004-04-20 James W. Overbeck Depositing fluid specimens on substrates, resulting ordered arrays, techniques for analysis of deposited arrays
WO1999036559A1 (en) 1998-01-13 1999-07-22 Julien Jean Pierre Viral vectors expressing self-polymerizing neuronal intermediate filaments and their use
WO2000017355A2 (en) 1998-09-18 2000-03-30 Incyte Pharmaceuticals, Inc. Human cytoskeleton associated proteins
US6232092B1 (en) 1998-10-02 2001-05-15 Rogers Imaging Corporation Method for preparing biological specimens for visual analysis
US6219575B1 (en) 1998-10-23 2001-04-17 Babak Nemati Method and apparatus to enhance optical transparency of biological tissues
US6409774B1 (en) 1999-06-11 2002-06-25 Resolution Sciences Corporation Electrophoresis-assisted staining of materials
JP2004512845A (ja) 2000-10-24 2004-04-30 オンコシス リミテッド ライアビリティ カンパニー 三次元の検体内の細胞を選択的に標的化する方法およびデバイス
AUPR378001A0 (en) * 2001-03-16 2001-04-12 Proteome Systems Ltd Protein chip
US6472216B1 (en) 2001-07-24 2002-10-29 Ann-Shyn Chiang Aqueous tissue clearing solution
CN1257752C (zh) * 2001-09-28 2006-05-31 生物药开发有限公司 多功能生物相容性水凝胶及其制备方法
DE10209609A1 (de) 2002-03-05 2003-10-02 Europ Lab Molekularbiolog Verfahren und Vorrichtung zur Parallelanalyse von Bio-Molekülen
EP1517715A2 (en) 2002-06-28 2005-03-30 Cardio, Inc. Decellularized tissue
US7319035B2 (en) 2002-10-17 2008-01-15 Vbi Technologies, L.L.C. Biological scaffolding material
JP4361057B2 (ja) * 2003-04-30 2009-11-11 モイル,ウィリアム,アール 生体分子検出および細胞分類用センサ
US7713923B2 (en) 2003-06-25 2010-05-11 Massachusetts Institute Of Technology Self-assembling peptides incorporating modifications and methods of use thereof
US8226715B2 (en) 2003-06-30 2012-07-24 Depuy Mitek, Inc. Scaffold for connective tissue repair
US7326571B2 (en) 2003-07-17 2008-02-05 Boston Scientific Scimed, Inc. Decellularized bone marrow extracellular matrix
US7660620B2 (en) 2003-09-29 2010-02-09 Medtronic, Inc. Timing techniques for magnetic resonance imaging
US7955788B2 (en) 2003-10-30 2011-06-07 Medtronic, Inc. Bioprosthetic tissue preparation with synthetic hydrogels
WO2005062938A2 (en) 2003-12-23 2005-07-14 The Regents Of The University Of California Chemical agents and methods for optical clearing of skin and mucous membranes to improve light-based diagnostics and therapeutics
ATE480550T1 (de) 2004-03-25 2010-09-15 California Inst Of Techn Hybridisierungskettenreaktion
US7452491B2 (en) * 2004-05-13 2008-11-18 Chung Yuan Christian University Method for forming scaffolds
GB0414825D0 (en) * 2004-07-02 2004-08-04 Biostatus Ltd Gel formulations and uses thereof
CN1256579C (zh) * 2004-09-23 2006-05-17 上海交通大学 基于原子力显微镜观察的生物样品包埋块的制备方法
US7727721B2 (en) 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
WO2007030012A2 (en) * 2005-09-05 2007-03-15 Stichting Voor De Technische Wetenscahappen Extracellular matrix imaging
EP1801573A1 (en) * 2005-12-21 2007-06-27 Boehringer Mannheim Gmbh Method and apparatus for parallel two-dimensional electrophoresis
EP2469263A1 (en) * 2006-01-13 2012-06-27 Ventana Medical Systems, Inc. Biological sample processing composition and method
EP2187985B1 (en) 2007-08-14 2016-09-28 Smith & Nephew PLC Scaffolds
DE102007045897A1 (de) 2007-09-26 2009-04-09 Carl Zeiss Microimaging Gmbh Verfahren zur mikroskopischen dreidimensionalen Abbildung einer Probe
US9134521B2 (en) 2008-07-30 2015-09-15 The Regents Of The University Of California Multidirectional selective plane illumination microscopy
US20100055733A1 (en) 2008-09-04 2010-03-04 Lutolf Matthias P Manufacture and uses of reactive microcontact printing of biomolecules on soft hydrogels
WO2010030358A1 (en) 2008-09-12 2010-03-18 Quickmbed, Inc. Scaffold for tissue sample orientation
WO2010094284A1 (en) * 2009-02-19 2010-08-26 Dako Denmark A/S Methods and compounds for detection of molecular targets
AU2010229858A1 (en) 2009-03-25 2011-09-22 Los Alamos National Security, Llc Fiber optical assembly for fluorescence spectrometry
CN102439398A (zh) * 2009-04-27 2012-05-02 蛋白质发现公司 可编程电泳凹口过滤器系统及方法
US8852614B2 (en) 2010-03-12 2014-10-07 The Board Of Trustees Of The Leland Stanford Junior University Hydrogels with network defects enhanced by nanoparticle incorporation
CN108061677B (zh) 2010-03-12 2021-10-29 国立研究开发法人理化学研究所 用于生物材料的澄清试剂和其用途
US8867803B2 (en) 2010-04-20 2014-10-21 Eric J. Seibel Optical projection tomography microscopy (OPTM) for large specimen sizes
JP5848331B2 (ja) 2010-05-14 2016-01-27 フジフィルム・イメイジング・カラランツ・リミテッド 分散物、分散物の調製方法およびインクジェット印刷インク
TWI425201B (zh) 2010-10-01 2014-02-01 Nat Univ Tsing Hua 觀察厚組織三維結構之方法
CN102072836B (zh) * 2010-11-12 2014-02-19 深圳华大基因科技有限公司 生物大分子浓缩除盐方法及试剂盒
US20140030192A1 (en) 2011-01-28 2014-01-30 Karl A. Deisseroth Functional Targeted Brain Endoskeletonization
WO2012147965A1 (ja) 2011-04-28 2012-11-01 独立行政法人理化学研究所 生物材料を透明化する方法、及びその利用
WO2012161143A1 (ja) 2011-05-20 2012-11-29 独立行政法人理化学研究所 生物材料用透明化試薬、及びその利用
US9359636B2 (en) 2011-07-27 2016-06-07 The Rockefeller University Methods for fixing and detecting RNA
JP5967528B2 (ja) 2012-06-22 2016-08-10 国立研究開発法人理化学研究所 生物材料を透明化する方法および生物材料用透明化処理キット
DE102012211462A1 (de) 2012-07-03 2014-01-23 Carl Zeiss Microscopy Gmbh Verfahren zur Vorbereitung und Durchführung der Aufnahme von Bildstapeln einer Probe aus verschiedenen Orientierungswinkeln
IN2014DN09073A (enExample) 2012-08-09 2015-05-22 Univ Leland Stanford Junior
DE102012020240A1 (de) 2012-10-12 2014-04-17 Carl Zeiss Microscopy Gmbh Mikroskop und Verfahren zur SPIM Mikroskopie
EP2992115B2 (en) 2013-04-30 2025-03-12 California Institute of Technology Multiplex labeling of molecules by sequential hybridization barcoding
WO2015022883A1 (ja) 2013-08-14 2015-02-19 独立行政法人理化学研究所 光透過性に優れた生物材料を調製するための組成物およびその利用
DE102013216934A1 (de) 2013-08-26 2015-02-26 Leica Microsystems Cms Gmbh Verfahren und Vorrichtung zur Erhöhung der optischen Transparenz von Bereichen einer Gewebeprobe
WO2015041755A1 (en) 2013-09-20 2015-03-26 California Institute Of Technology Methods for phenotyping of intact whole tissues
CN103513411B (zh) 2013-09-27 2016-02-03 香港应用科技研究院有限公司 荧光显微镜中聚焦的装置和方法
JP2017517761A (ja) 2014-05-30 2017-06-29 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 大きなインタクトな組織試料を撮像するための方法及び装置
WO2016023009A1 (en) 2014-08-07 2016-02-11 Wake Forest University Health Sciences Compositions and methods for clearing a biological sample
WO2016073941A1 (en) 2014-11-07 2016-05-12 California Institute Of Technology Methods for phenotyping of intact bones by tissue clearing and staining
WO2016117614A1 (ja) 2015-01-20 2016-07-28 国立研究開発法人理化学研究所 生物材料用透明化試薬、システム及びその利用
EP3273218A4 (en) 2015-03-18 2018-12-26 Riken Method for observing biological material and clearing method
US20190113423A1 (en) 2015-12-02 2019-04-18 Clearlight Diagnostics, LLC Methods for preparing and analyzing tumor tissue samples for detection and monitoring of cancers

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070134798A1 (en) * 2005-12-08 2007-06-14 Mccormick Patric Light curing fixative

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ALBRECHT, D. R., et al., "Photo- and electropatterning of hydrogel-encapsulated living cell arrays", Lab on a Chip, 2005, Volume 5, pages 111-118. *
OOSHUYSEN, A., et al., "Bioprosthetic tissue preservation by filling with a poly(acrylamide) hydrogel", Biomaterials, 2006, Vol. 27, pages 2123-2130. *

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