SK7702000A3 - Process for preparing coryneform bacteria having an improved amino acid- or nucleic acid-productivity - Google Patents
Process for preparing coryneform bacteria having an improved amino acid- or nucleic acid-productivity Download PDFInfo
- Publication number
- SK7702000A3 SK7702000A3 SK770-2000A SK7702000A SK7702000A3 SK 7702000 A3 SK7702000 A3 SK 7702000A3 SK 7702000 A SK7702000 A SK 7702000A SK 7702000 A3 SK7702000 A3 SK 7702000A3
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- amino acid
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/14—Glutamic acid; Glutamine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
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- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
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- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
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JP27178698 | 1998-09-25 | ||
JP27178798 | 1998-09-25 | ||
PCT/JP1999/005175 WO2000018935A1 (fr) | 1998-09-25 | 1999-09-22 | Procede de construction d'une bacterie produisant des acides amines, et procede de production d'acides amines par une technique de fermentation utilisant ladite bacterie |
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SK7702000A3 true SK7702000A3 (en) | 2000-10-09 |
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SK770-2000A SK7702000A3 (en) | 1998-09-25 | 1999-09-22 | Process for preparing coryneform bacteria having an improved amino acid- or nucleic acid-productivity |
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US (2) | US6962805B2 (zh) |
EP (1) | EP1033407B1 (zh) |
KR (1) | KR100630604B1 (zh) |
CN (1) | CN1170938C (zh) |
AT (1) | ATE447039T1 (zh) |
BR (2) | BRPI9909409B1 (zh) |
DE (1) | DE69941594D1 (zh) |
ES (1) | ES2335828T3 (zh) |
HU (2) | HU225541B1 (zh) |
ID (1) | ID25513A (zh) |
MY (1) | MY130756A (zh) |
PE (1) | PE20001111A1 (zh) |
RU (1) | RU2214456C2 (zh) |
SK (1) | SK7702000A3 (zh) |
TW (4) | TWI247808B (zh) |
WO (1) | WO2000018935A1 (zh) |
Families Citing this family (135)
Publication number | Priority date | Publication date | Assignee | Title |
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ZA964665B (en) | 1995-06-07 | 1997-01-07 | Ajinomoto Kk | Method of producing l-lysine |
CN1170938C (zh) | 1998-09-25 | 2004-10-13 | 味之素株式会社 | 构建产生氨基酸的细菌的方法及通过发酵该经构建的产生氨基酸的细菌以制备氨基酸的方法 |
AU2001285833A1 (en) * | 2000-07-24 | 2002-02-05 | Degussa A.G. | Process for the fermentative preparation of l-glutamic acid using coryneform bacteria |
AU783498B2 (en) * | 2000-11-17 | 2005-11-03 | Cj Cheiljedang Corporation | Microorganisms producing L-glutamine and processes for producing L-glutamine using the same |
BR0304860A (pt) * | 2002-11-11 | 2004-08-31 | Ajinomoto Kk | Método para produzir uma substância alvo pela utilização de uma-bactéria pertencente ao gênero escherichia |
DE60335774D1 (de) * | 2002-11-26 | 2011-03-03 | Ajinomoto Kk | Methode für die Produktion von L-Glutamin und L-Glutamin-produzierendes Bakterium |
DE10359594A1 (de) * | 2003-12-18 | 2005-07-28 | Basf Ag | PEF-TU-Expressionseinheiten |
DE602005016763D1 (de) * | 2004-01-30 | 2009-11-05 | Ajinomoto Kk | L-aminosäure produzierender mikroorganismus und verfahren zur l-aminosäureproduktion |
JP4760711B2 (ja) | 2004-03-31 | 2011-08-31 | 味の素株式会社 | バチルス属又はエシェリヒア属に属する細菌を使用した発酵によるプリンヌクレオシド及びヌクレオチドの製造方法 |
DE102004035065A1 (de) | 2004-07-20 | 2006-02-16 | Basf Ag | P-ET-TS-Expressionseinheiten |
JP2006063002A (ja) * | 2004-08-25 | 2006-03-09 | Ajinomoto Co Inc | 反芻動物用のメタン生成抑制剤および飼料用組成物 |
EP2602320B1 (en) * | 2004-10-13 | 2016-11-30 | Mitsui Chemicals, Inc. | DNA encoding novel enzyme having D-serine synthase activity, method of producing the enzyme and method of producing D-serine by using the same |
JP4595506B2 (ja) | 2004-11-25 | 2010-12-08 | 味の素株式会社 | L−アミノ酸生産菌及びl−アミノ酸の製造方法 |
KR100620092B1 (ko) * | 2004-12-16 | 2006-09-08 | 씨제이 주식회사 | 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법 |
BR122019024048B1 (pt) | 2004-12-28 | 2022-05-24 | Ajinomoto Co., Inc | Método para produzir ácido l-glutâmico |
US7794989B2 (en) * | 2004-12-28 | 2010-09-14 | Ajinomoto Co., Inc. | L-glutamic acid-producing microorganism and a method for producing L-glutamic acid |
US7326546B2 (en) | 2005-03-10 | 2008-02-05 | Ajinomoto Co., Inc. | Purine-derived substance-producing bacterium and a method for producing purine-derived substance |
TWI361834B (en) | 2005-04-12 | 2012-04-11 | Kyowa Hakko Bio Co Ltd | A method for producing amino acids |
US20070029252A1 (en) * | 2005-04-12 | 2007-02-08 | Dunson James B Jr | System and process for biomass treatment |
US7781191B2 (en) * | 2005-04-12 | 2010-08-24 | E. I. Du Pont De Nemours And Company | Treatment of biomass to obtain a target chemical |
JP5804666B2 (ja) * | 2005-04-12 | 2015-11-04 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーE.I.Du Pont De Nemours And Company | バイオマスの処理および利用における別の供給流れの集中 |
JP2008283863A (ja) * | 2005-08-26 | 2008-11-27 | Ajinomoto Co Inc | L−アミノ酸生産菌及びl−アミノ酸の製造方法 |
BRPI0617491B1 (pt) | 2005-10-18 | 2021-04-27 | Ajinomoto Co., Inc | Processo para produção de ácido succínico |
BRPI0710752B1 (pt) | 2006-04-24 | 2017-01-24 | Ajinomoto Kk | métodos para produzir uma substância derivada de purina e um nucleotídeo de purina |
CN101432418B (zh) | 2006-04-24 | 2012-11-14 | 味之素株式会社 | 能够产生嘌呤物质的细菌和用于产生嘌呤物质的方法 |
EP2021458A1 (en) | 2006-05-23 | 2009-02-11 | Ajinomoto Co., Inc. | A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family |
EP2066799B1 (en) | 2006-09-28 | 2013-12-11 | Ajinomoto Co., Inc. | Method for producing 4-hydroxy-l-isoleucine |
KR100822041B1 (ko) * | 2006-12-21 | 2008-04-15 | 씨제이제일제당 (주) | 몰리브덴 보조인자 생합성 효소 에이 코딩 유전자의 발현이강화된 코리네박테리움 속 미생물 및 이를 이용한엘-라이신 생산방법 |
KR100882418B1 (ko) | 2007-01-15 | 2009-02-05 | 씨제이제일제당 (주) | 이노신을 생산하는 미생물 및 그를 이용한 이노신 제조방법 |
WO2008090770A1 (ja) | 2007-01-22 | 2008-07-31 | Ajinomoto Co., Inc. | L-アミノ酸を生産する微生物及びl-アミノ酸の製造法 |
JP2010088301A (ja) * | 2007-02-01 | 2010-04-22 | Ajinomoto Co Inc | L−アミノ酸の製造法 |
JP2010110217A (ja) * | 2007-02-22 | 2010-05-20 | Ajinomoto Co Inc | L−アミノ酸生産菌及びl−アミノ酸の製造法 |
JP2010130899A (ja) | 2007-03-14 | 2010-06-17 | Ajinomoto Co Inc | L−グルタミン酸系アミノ酸生産微生物及びアミノ酸の製造法 |
EP2149608A4 (en) | 2007-04-16 | 2013-02-20 | Ajinomoto Kk | PROCESS FOR PRODUCING AN ORGANIC ACID |
JP5218400B2 (ja) | 2007-04-17 | 2013-06-26 | 味の素株式会社 | カルボキシル基を有する酸性物質の製造法 |
US20090053800A1 (en) * | 2007-08-22 | 2009-02-26 | Julie Friend | Biomass Treatment Apparatus |
US7819976B2 (en) * | 2007-08-22 | 2010-10-26 | E. I. Du Pont De Nemours And Company | Biomass treatment method |
US8445236B2 (en) * | 2007-08-22 | 2013-05-21 | Alliance For Sustainable Energy Llc | Biomass pretreatment |
US7807419B2 (en) * | 2007-08-22 | 2010-10-05 | E. I. Du Pont De Nemours And Company | Process for concentrated biomass saccharification |
JP2010263790A (ja) * | 2007-09-04 | 2010-11-25 | Ajinomoto Co Inc | アミノ酸生産微生物及びアミノ酸の製造法 |
RU2396336C2 (ru) | 2007-09-27 | 2010-08-10 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) | СПОСОБ ПОЛУЧЕНИЯ АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ, ПРИНАДЛЕЖАЩЕЙ К РОДУ Escherichia |
JP5644108B2 (ja) | 2007-12-06 | 2014-12-24 | 味の素株式会社 | 有機酸の製造方法 |
JP2011067095A (ja) | 2008-01-10 | 2011-04-07 | Ajinomoto Co Inc | 発酵法による目的物質の製造法 |
JP5526785B2 (ja) | 2008-01-23 | 2014-06-18 | 味の素株式会社 | L−アミノ酸の製造法 |
WO2009154280A1 (ja) * | 2008-06-19 | 2009-12-23 | 味の素株式会社 | 過食防止剤 |
JP5488467B2 (ja) | 2008-09-05 | 2014-05-14 | 味の素株式会社 | L−アミノ酸生産菌及びl−アミノ酸の製造法 |
WO2010027045A1 (ja) | 2008-09-08 | 2010-03-11 | 味の素株式会社 | L-アミノ酸を生産する微生物及びl-アミノ酸の製造法 |
JP2012029565A (ja) | 2008-11-27 | 2012-02-16 | Ajinomoto Co Inc | L−アミノ酸の製造法 |
JPWO2011013707A1 (ja) | 2009-07-29 | 2013-01-10 | 味の素株式会社 | L−アミノ酸の製造法 |
JP5636648B2 (ja) | 2009-08-10 | 2014-12-10 | 味の素株式会社 | 5’−グアニル酸の製造法 |
JP2012223091A (ja) | 2009-08-25 | 2012-11-15 | Ajinomoto Co Inc | L−アミノ酸の製造法 |
JP2012223092A (ja) | 2009-08-28 | 2012-11-15 | Ajinomoto Co Inc | L−アミノ酸の製造法 |
JP2013013329A (ja) | 2009-11-06 | 2013-01-24 | Ajinomoto Co Inc | L−アミノ酸の製造法 |
JP2013074795A (ja) | 2010-02-08 | 2013-04-25 | Ajinomoto Co Inc | 変異型rpsA遺伝子及びL−アミノ酸の製造法 |
KR101226384B1 (ko) * | 2010-03-05 | 2013-01-25 | 씨제이제일제당 (주) | 개량된 프로모터 및 이를 이용한 l-라이신의 생산 방법 |
US8721794B2 (en) | 2010-04-28 | 2014-05-13 | E I Du Pont De Nemours And Company | Production of high solids syrup from lignocellulosic biomass hydrolysate fermentation broth |
US8906235B2 (en) | 2010-04-28 | 2014-12-09 | E I Du Pont De Nemours And Company | Process for liquid/solid separation of lignocellulosic biomass hydrolysate fermentation broth |
RU2501858C2 (ru) | 2010-07-21 | 2013-12-20 | Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) | СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТЫ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА Enterobacteriaceae |
JP5932649B2 (ja) | 2010-09-08 | 2016-06-08 | グリーンフェノール開発株式会社 | コリネ型細菌形質転換体及びそれを用いるフェノールの製造方法 |
WO2012036151A1 (ja) | 2010-09-14 | 2012-03-22 | 味の素株式会社 | 含硫アミノ酸生産菌及び含硫アミノ酸の製造法 |
CN103221533B (zh) | 2010-11-10 | 2017-08-22 | 绿色苯酚开发株式会社 | 棒状细菌转化体及使用该转化体的苯酚的制造方法 |
JP2014036576A (ja) | 2010-12-10 | 2014-02-27 | Ajinomoto Co Inc | L−アミノ酸の製造法 |
JP2014087259A (ja) | 2011-02-22 | 2014-05-15 | Ajinomoto Co Inc | L−システイン生産菌及びl−システインの製造法 |
EP2687591B1 (en) | 2011-03-18 | 2021-01-06 | Mitsubishi Chemical Corporation | Method for producing polymer, method for producing organic acid, and organic acid-producing microorganism |
WO2012137689A1 (ja) | 2011-04-01 | 2012-10-11 | 味の素株式会社 | L-システインの製造法 |
DE102011006716A1 (de) | 2011-04-04 | 2012-10-04 | Evonik Degussa Gmbh | Mikroorganismus und Verfahren zur fermentativen Herstellung einer organisch-chemischen Verbindung |
JP2014131487A (ja) | 2011-04-18 | 2014-07-17 | Ajinomoto Co Inc | L−システインの製造法 |
RU2496867C2 (ru) | 2011-04-25 | 2013-10-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | Способ получения l-аминокислоты семейства глутамата с использованием коринеформной бактерии |
AU2012333515B2 (en) | 2011-11-02 | 2015-07-16 | Ajinomoto Co., Inc. | Method for secreting and producing proteins |
EP2778228B1 (en) | 2011-11-11 | 2017-05-17 | Ajinomoto Co., Inc. | Method for producing 2-ketoglutaric acid and derivatives thereof by using a bacterium from the genus pantoea or the genus corynebacterium |
RU2012129311A (ru) | 2012-07-11 | 2014-01-20 | Закрытое акционерное общество " Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | Днк, кодирующая дипептид-синтезирующий фермер (варианты), бактерия рода escherichia и способ получения дипептидов с ее использованием |
RU2550269C2 (ru) | 2012-08-17 | 2015-05-10 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | СПОСОБ ПОЛУЧЕНИЯ L-АРГИНИНА С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА Enterobacteriaceae, СОДЕРЖАЩЕЙ N-АЦЕТИЛОРНИТИНДЕАЦЕТИЛАЗУ С НАРУШЕННОЙ АКТИВНОСТЬЮ |
EP2762571A1 (de) | 2013-01-30 | 2014-08-06 | Evonik Industries AG | Mikroorganismus und Verfahren zur fermentativen Herstellung von Aminosäuren |
JP6335196B2 (ja) | 2013-02-08 | 2018-05-30 | ニンシャー エッペン バイオテック カンパニー リミテッド | アコニターゼ遺伝子および/またはその調節エレメントの改変により、l−リジンを生産する方法 |
US20140273105A1 (en) | 2013-03-12 | 2014-09-18 | E I Du Pont De Nemours And Company | Gradient pretreatment of lignocellulosic biomass |
WO2014160262A1 (en) | 2013-03-14 | 2014-10-02 | Abengoa Bioenergy New Technologies, Llc | Methods for converting cellulosic waste to bioproducts |
RU2013118637A (ru) | 2013-04-23 | 2014-10-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА ENTEROBACTERIACEAE, В КОТОРОЙ РАЗРЕГУЛИРОВАН ГЕН yjjK |
RU2013119826A (ru) | 2013-04-29 | 2014-11-10 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | Коринеформная бактерия и способ получения гетерологичных гибридных белков |
MY185322A (en) | 2013-05-13 | 2021-05-04 | Ajinomoto Kk | Method for producing l-amino acid |
SG10201707552VA (en) * | 2013-05-17 | 2017-11-29 | Xyleco Inc | Processing biomass |
JP2016165225A (ja) | 2013-07-09 | 2016-09-15 | 味の素株式会社 | 有用物質の製造方法 |
RU2013140115A (ru) | 2013-08-30 | 2015-03-10 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА Enterobacteriaceae, В КОТОРОЙ НАРУШЕНА ЭКСПРЕССИЯ КЛАСТЕРА ГЕНОВ znuACB |
JP2016192903A (ja) | 2013-09-17 | 2016-11-17 | 味の素株式会社 | 海藻由来バイオマスからのl−アミノ酸の製造方法 |
JP6569530B2 (ja) | 2013-10-02 | 2019-09-04 | 味の素株式会社 | ヘパロサン生産細菌及びヘパロサンの製造法 |
PL3053999T3 (pl) | 2013-10-02 | 2020-03-31 | Ajinomoto Co., Inc. | Przyrząd do regulowania poziomu amoniaku i sposób regulowania poziomu amoniaku |
RU2013144250A (ru) | 2013-10-02 | 2015-04-10 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА Enterobacteriaceae, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНА, КОДИРУЮЩЕГО ФОСФАТНЫЙ ТРАНСПОРТЕР |
WO2015060314A1 (ja) | 2013-10-21 | 2015-04-30 | 味の素株式会社 | L-アミノ酸の製造法 |
BR112016008830B1 (pt) | 2013-10-23 | 2023-02-23 | Ajinomoto Co., Inc | Método para produzir uma substância alvo |
US20150147786A1 (en) | 2013-11-24 | 2015-05-28 | E I Du Pont De Nemours And Company | High force and high stress destructuring for starch biomass processing |
EP3101130B1 (en) | 2014-01-31 | 2020-05-06 | Ajinomoto Co., Inc. | Mutant glutamate-cysteine ligase and method for manufacturing gamma-glutamyl-valyl-glycine |
WO2016007350A1 (en) | 2014-07-09 | 2016-01-14 | Danisco Us Inc. | Preconditioning of lignocellulosic biomass |
US20170327850A1 (en) * | 2014-10-30 | 2017-11-16 | Board Of Regents, The University Of Texas System | Engineered fungi for itaconic acid production |
CN104513830B (zh) * | 2015-01-27 | 2017-08-11 | 华东理工大学 | 一种适用于氧化葡萄糖酸杆菌的基因表达载体及其应用 |
CN108026548A (zh) | 2015-05-19 | 2018-05-11 | 卢塞特英国国际有限公司 | 生物制备甲基丙烯酸及其衍生物的方法 |
RU2015120052A (ru) | 2015-05-28 | 2016-12-20 | Аджиномото Ко., Инк. | Способ получения L-аминокислоты с использованием бактерии семейства Enterobacteriaceae, в которой ослаблена экспрессия гена gshA |
EP3368695A2 (en) | 2015-10-27 | 2018-09-05 | Ajinomoto Co., Inc. | Method for producing aldehyde |
JP6623690B2 (ja) | 2015-10-30 | 2019-12-25 | 味の素株式会社 | グルタミン酸系l−アミノ酸の製造法 |
EP3402891A1 (en) | 2016-01-12 | 2018-11-21 | Ajinomoto Co., Inc. | Method for producing benzaldehyde |
JP7021639B2 (ja) | 2016-10-21 | 2022-02-17 | 味の素株式会社 | タンパク質の分泌生産法 |
CN109890970B (zh) | 2016-10-26 | 2023-04-04 | 味之素株式会社 | 生产目标物质的方法 |
WO2018079684A1 (en) | 2016-10-26 | 2018-05-03 | Ajinomoto Co., Inc. | Method for producing objective substance |
GB201619827D0 (en) | 2016-11-23 | 2017-01-04 | Lucite Int Uk Ltd | Process for the production of methyl methacrylate |
BR112020005799A2 (pt) | 2017-09-25 | 2020-09-24 | Ajinomoto Co., Inc. | métodos para produzir uma proteína alvo e um dissacarídeo. |
WO2019078095A1 (en) | 2017-10-16 | 2019-04-25 | Ajinomoto Co., Inc. | PROCESS FOR PRODUCTION OF PROTEIN HAVING ALPHA-HYDROXYLANTIC PEPTIDYLGLYCIN MONOOXYGENASE ACTIVITY |
US11680279B2 (en) | 2017-11-29 | 2023-06-20 | Ajinomoto Co., Inc. | Method for producing objective substance |
EP3732298A1 (en) | 2017-12-26 | 2020-11-04 | Ajinomoto Co., Inc. | Method of producing glycine by fermentation |
JP7367667B2 (ja) | 2018-02-20 | 2023-10-24 | 味の素株式会社 | Rnaサイレンシングを誘導する方法 |
JP7124338B2 (ja) | 2018-02-27 | 2022-08-24 | 味の素株式会社 | 変異型グルタチオン合成酵素、及び、γ-グルタミルバリルグリシンの製造法 |
EP3821025B1 (en) | 2018-07-11 | 2023-09-20 | Ajinomoto Co., Inc. | Method for enzymatic sulfurylation of alcohols and amines using bacterium of the family enterobacteriaceae |
WO2020027251A1 (en) | 2018-08-03 | 2020-02-06 | Ajinomoto Co., Inc. | Method for producing objective substance |
WO2020067487A1 (en) | 2018-09-28 | 2020-04-02 | Ajinomoto Co., Inc. | Method for producing l-methionine using a bacterium |
WO2020071538A1 (en) | 2018-10-05 | 2020-04-09 | Ajinomoto Co., Inc. | Method for producing target substance by bacterial fermentation |
CN112930359A (zh) | 2018-10-25 | 2021-06-08 | 味之素株式会社 | 蛋白质的分泌生产方法 |
KR102075160B1 (ko) | 2018-12-13 | 2020-02-10 | 대상 주식회사 | L-글루탐산 생산능이 향상된 변이 균주 및 이를 이용한 l-글루탐산의 제조 방법 |
JP7491312B2 (ja) | 2018-12-27 | 2024-05-28 | 味の素株式会社 | 腸内細菌科の細菌の発酵による塩基性l-アミノ酸またはその塩の製造方法 |
JP7491314B2 (ja) | 2019-02-22 | 2024-05-28 | 味の素株式会社 | ydiJ遺伝子を過剰発現する腸内細菌科に属する細菌を用いたL-アミノ酸の製造方法 |
WO2020204179A1 (en) | 2019-04-05 | 2020-10-08 | Ajinomoto Co., Inc. | Method of producing l-amino acids |
WO2021060438A1 (en) | 2019-09-25 | 2021-04-01 | Ajinomoto Co., Inc. | Method for producing l-amino acids by bacterial fermentation |
JP2022550349A (ja) | 2019-09-25 | 2022-12-01 | 味の素株式会社 | 細菌の発酵による2-メチル酪酸の製造方法 |
JPWO2021085405A1 (zh) | 2019-10-28 | 2021-05-06 | ||
CN110951662B (zh) * | 2019-12-26 | 2024-03-12 | 新疆梅花氨基酸有限责任公司 | 一种高产赖氨酸的棒状细菌及其构建方法与应用 |
KR102269639B1 (ko) | 2020-02-12 | 2021-06-25 | 대상 주식회사 | L-글루탐산 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-글루탐산의 생산 방법 |
CN111334535B (zh) * | 2020-03-26 | 2021-11-30 | 廊坊梅花生物技术开发有限公司 | 生产l-氨基酸的重组菌株及其应用 |
CN113201535B (zh) * | 2020-07-20 | 2022-02-08 | 中国科学院天津工业生物技术研究所 | 谷氨酸脱氢酶基因启动子的突变体及其应用 |
WO2022071061A1 (ja) | 2020-09-29 | 2022-04-07 | 味の素株式会社 | 変異型トランスグルタミナーゼ |
CN116583605A (zh) | 2020-10-28 | 2023-08-11 | 味之素株式会社 | L-氨基酸的制造方法 |
CN112695036B (zh) * | 2021-03-23 | 2021-07-06 | 中国科学院天津工业生物技术研究所 | 一种天冬氨酸激酶基因表达调控序列及其应用 |
AU2022258873A1 (en) | 2021-04-16 | 2023-11-30 | Inbiose N.V. | Fermentative production |
CN117157396A (zh) | 2021-04-16 | 2023-12-01 | 因比奥斯公司 | 唾液酸化的二糖和/或寡糖的细胞生产 |
KR102377745B1 (ko) * | 2021-05-12 | 2022-03-23 | 씨제이제일제당 주식회사 | 신규 프로모터 및 이의 용도 |
CN113308426B (zh) * | 2021-05-27 | 2022-10-18 | 齐鲁工业大学 | 一种改造tk基因5′端序列的重组棒状杆菌及其应用 |
AU2022306337A1 (en) | 2021-07-07 | 2024-02-01 | Ajinomoto Co., Inc. | Method for secretory production of unnatural-amino-acid-containing protein |
CA3228922A1 (en) | 2021-10-11 | 2023-04-20 | Ajinomoto Co., Inc. | Bacterium modified to express heterologous tat protein |
GB2621337A (en) | 2022-08-08 | 2024-02-14 | Mitsubishi Chemical Uk Ltd | Process for the biological production of methacrylic acid and derivatives thereof |
US20240117393A1 (en) | 2022-09-30 | 2024-04-11 | Ajinomoto Co., Inc. | Method for producing l-amino acid |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
LU81207A1 (fr) | 1979-04-30 | 1980-12-16 | Arbed | Procede d'affinage d'un bain de metal contenant des matieres refroidissantes solides |
JPS61104790A (ja) | 1984-10-29 | 1986-05-23 | Showa Denko Kk | L−トリプトフアンの生合成に関与する遺伝情報を有する新規組換え体dna |
JP2520895B2 (ja) * | 1987-03-04 | 1996-07-31 | 旭化成工業株式会社 | L―グルタミン酸の製造方法 |
JP2708168B2 (ja) | 1988-02-23 | 1998-02-04 | 昭和電工株式会社 | 微生物の改良 |
EP0551506A1 (fr) * | 1991-07-30 | 1993-07-21 | Orsan | Systeme d'expression et de secretion de proteines utilisables en particulier chez les corynebacteries |
ES2160167T3 (es) * | 1994-06-14 | 2001-11-01 | Ajinomoto Kk | Gen de alfa-cetoglutarato-deshidrogenasa. |
WO1998007846A1 (en) | 1996-08-23 | 1998-02-26 | Peter Ruhdal Jensen | Artificial promoter libraries for selected organisms and promoters derived from such libraries |
CN1170938C (zh) | 1998-09-25 | 2004-10-13 | 味之素株式会社 | 构建产生氨基酸的细菌的方法及通过发酵该经构建的产生氨基酸的细菌以制备氨基酸的方法 |
US6255086B1 (en) * | 1999-02-01 | 2001-07-03 | Ajinomoto Co., Inc. | Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine |
US7160705B2 (en) * | 2000-04-28 | 2007-01-09 | Ajinomoto Co., Inc. | Arginine repressor deficient strain of coryneform bacterium and method for producing L-arginine |
-
1999
- 1999-09-22 CN CNB998023809A patent/CN1170938C/zh not_active Expired - Lifetime
- 1999-09-22 DE DE69941594T patent/DE69941594D1/de not_active Expired - Lifetime
- 1999-09-22 ES ES99944770T patent/ES2335828T3/es not_active Expired - Lifetime
- 1999-09-22 RU RU2000112926/13A patent/RU2214456C2/ru not_active IP Right Cessation
- 1999-09-22 WO PCT/JP1999/005175 patent/WO2000018935A1/ja active IP Right Grant
- 1999-09-22 EP EP99944770A patent/EP1033407B1/en not_active Expired - Lifetime
- 1999-09-22 HU HU0100045D patent/HU225541B1/hu unknown
- 1999-09-22 HU HU0100045A patent/HU224975B1/hu unknown
- 1999-09-22 KR KR1020007005666A patent/KR100630604B1/ko not_active IP Right Cessation
- 1999-09-22 BR BRPI9909409A patent/BRPI9909409B1/pt active IP Right Grant
- 1999-09-22 AT AT99944770T patent/ATE447039T1/de not_active IP Right Cessation
- 1999-09-22 BR BRPI9917880-0A patent/BR9917880B1/pt active IP Right Grant
- 1999-09-22 SK SK770-2000A patent/SK7702000A3/sk unknown
- 1999-09-22 ID IDW20000964A patent/ID25513A/id unknown
- 1999-09-24 PE PE1999000974A patent/PE20001111A1/es not_active Application Discontinuation
- 1999-09-24 MY MYPI99004141A patent/MY130756A/en unknown
- 1999-09-27 TW TW093129855A patent/TWI247808B/zh not_active IP Right Cessation
- 1999-09-27 TW TW088116544A patent/TWI247805B/zh not_active IP Right Cessation
- 1999-09-27 TW TW093129853A patent/TWI247807B/zh not_active IP Right Cessation
- 1999-09-27 TW TW093129852A patent/TWI247806B/zh not_active IP Right Cessation
-
2000
- 2000-05-25 US US09/577,005 patent/US6962805B2/en not_active Expired - Lifetime
-
2005
- 2005-09-06 US US11/218,433 patent/US7608437B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US20060003424A1 (en) | 2006-01-05 |
MY130756A (en) | 2007-07-31 |
PE20001111A1 (es) | 2000-10-31 |
US20040002143A1 (en) | 2004-01-01 |
US7608437B2 (en) | 2009-10-27 |
CN1289368A (zh) | 2001-03-28 |
EP1033407A1 (en) | 2000-09-06 |
TW200504215A (en) | 2005-02-01 |
TWI247807B (en) | 2006-01-21 |
HU225541B1 (en) | 2007-03-28 |
ATE447039T1 (de) | 2009-11-15 |
HUP0100045A2 (hu) | 2001-05-28 |
BRPI9909409B1 (pt) | 2016-03-22 |
TWI247808B (en) | 2006-01-21 |
TWI247805B (en) | 2006-01-21 |
KR20010032426A (ko) | 2001-04-16 |
ES2335828T3 (es) | 2010-04-05 |
TWI247806B (en) | 2006-01-21 |
TW200504213A (en) | 2005-02-01 |
BR9917880B1 (pt) | 2014-02-18 |
WO2000018935A1 (fr) | 2000-04-06 |
HUP0100045A3 (en) | 2003-01-28 |
HU224975B1 (en) | 2006-04-28 |
ID25513A (id) | 2000-10-05 |
DE69941594D1 (de) | 2009-12-10 |
BR9909409A (pt) | 2000-11-21 |
TW200504214A (en) | 2005-02-01 |
CN1170938C (zh) | 2004-10-13 |
EP1033407A4 (en) | 2004-08-18 |
US6962805B2 (en) | 2005-11-08 |
KR100630604B1 (ko) | 2006-10-04 |
RU2214456C2 (ru) | 2003-10-20 |
EP1033407B1 (en) | 2009-10-28 |
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