PL2393917T3 - Podłoże do hodowli nabłonkowych komórek macierzystych i organoidów zawierających komórki macierzyste - Google Patents

Podłoże do hodowli nabłonkowych komórek macierzystych i organoidów zawierających komórki macierzyste

Info

Publication number
PL2393917T3
PL2393917T3 PL10703131.2T PL10703131T PL2393917T3 PL 2393917 T3 PL2393917 T3 PL 2393917T3 PL 10703131 T PL10703131 T PL 10703131T PL 2393917 T3 PL2393917 T3 PL 2393917T3
Authority
PL
Poland
Prior art keywords
stem cells
organoids
culture medium
epithelial
epithelial stem
Prior art date
Application number
PL10703131.2T
Other languages
English (en)
Inventor
Toshiro Sato
Johannes Carolus Clevers
Ortega Meritxell Huch
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=42144924&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PL2393917(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed filed Critical
Publication of PL2393917T3 publication Critical patent/PL2393917T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0677—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Diabetes (AREA)
  • Emergency Medicine (AREA)
  • Endocrinology (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Developmental Biology & Embryology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
PL10703131.2T 2009-02-03 2010-02-03 Podłoże do hodowli nabłonkowych komórek macierzystych i organoidów zawierających komórki macierzyste PL2393917T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14962209P 2009-02-03 2009-02-03
EP09151970 2009-02-03
EP09171831 2009-09-30

Publications (1)

Publication Number Publication Date
PL2393917T3 true PL2393917T3 (pl) 2016-12-30

Family

ID=42144924

Family Applications (3)

Application Number Title Priority Date Filing Date
PL10703131.2T PL2393917T3 (pl) 2009-02-03 2010-02-03 Podłoże do hodowli nabłonkowych komórek macierzystych i organoidów zawierających komórki macierzyste
PL18182285.9T PL3441458T3 (pl) 2009-02-03 2010-02-03 Podłoże do hodowli nabłonkowych komórek macierzystych i organoidów zawierających wspomniane komórki macierzyste
PL16151949T PL3061808T3 (pl) 2009-02-03 2010-02-03 Podłoże do hodowli nabłonkowych komórek macierzystych i organoidów zawierających wspomniane komórki macierzyste

Family Applications After (2)

Application Number Title Priority Date Filing Date
PL18182285.9T PL3441458T3 (pl) 2009-02-03 2010-02-03 Podłoże do hodowli nabłonkowych komórek macierzystych i organoidów zawierających wspomniane komórki macierzyste
PL16151949T PL3061808T3 (pl) 2009-02-03 2010-02-03 Podłoże do hodowli nabłonkowych komórek macierzystych i organoidów zawierających wspomniane komórki macierzyste

Country Status (18)

Country Link
US (3) US8642339B2 (pl)
EP (4) EP2393917B1 (pl)
JP (2) JP5458112B2 (pl)
KR (1) KR101904224B1 (pl)
CN (1) CN102439135B (pl)
AU (1) AU2010211428B2 (pl)
CA (1) CA2751332C (pl)
DK (2) DK2393917T3 (pl)
ES (2) ES2579909T3 (pl)
HR (2) HRP20230650T1 (pl)
HU (2) HUE028647T2 (pl)
IL (1) IL214381A (pl)
MX (3) MX2011008044A (pl)
NZ (1) NZ594271A (pl)
PL (3) PL2393917T3 (pl)
RU (1) RU2555545C2 (pl)
SG (1) SG173492A1 (pl)
WO (1) WO2010090513A2 (pl)

Families Citing this family (150)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2022848A1 (en) 2007-08-10 2009-02-11 Hubrecht Institut A method for identifying, expanding, and removing adult stem cells and cancer stem cells
US9464275B2 (en) * 2008-08-21 2016-10-11 The Board Of Trustees Of The Leland Stanford Junior University Ex vivo culture, proliferation and expansion of intestinal epithelium
EP2412800A1 (en) * 2010-07-29 2012-02-01 Koninklijke Nederlandse Akademie van Wetenschappen Liver organoid, uses thereof and culture method for obtaining them
GB201111244D0 (en) * 2011-06-30 2011-08-17 Konink Nl Akademie Van Wetenschappen Knaw Culture media for stem cells
US9752124B2 (en) 2009-02-03 2017-09-05 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium for epithelial stem cells and organoids comprising the stem cells
US8642339B2 (en) * 2009-02-03 2014-02-04 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium for epithelial stem cells and organoids comprising the stem cells
US8563307B2 (en) 2009-02-24 2013-10-22 James Wang Treatment of immunosuppression-related disorders
US9345486B2 (en) 2009-03-16 2016-05-24 University Of Washington Nanofibrous conduits for nerve regeneration
WO2011140441A2 (en) 2010-05-06 2011-11-10 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
US8679474B2 (en) 2010-08-04 2014-03-25 StemBios Technologies, Inc. Somatic stem cells
WO2012030854A2 (en) 2010-09-01 2012-03-08 Whitehead Institute For Biomedical Research Compositions and methods for modulating emt and uses thereof
JP5850419B2 (ja) * 2010-11-11 2016-02-03 国立大学法人大阪大学 細胞の三次元構造体、及び、これを製造する方法
EP2681306B1 (en) 2011-02-28 2019-01-09 President and Fellows of Harvard College Cell culture system
GB201106395D0 (en) * 2011-04-14 2011-06-01 Hubrecht Inst Compounds
CN102787094B (zh) * 2011-05-17 2015-09-23 李晖 培养基、细胞培养用试剂盒及细胞培养方法
DK2756075T3 (da) * 2011-08-29 2019-12-09 Inst Nat Sante Rech Med Fremgangsmåde til fremstilling af inducerede paraksiale mesoderme progenitor (IPAM)-celler og anvendelse deraf
TWI614340B (zh) 2011-09-28 2018-02-11 幹細胞生物科技股份有限公司 體幹細胞及其製備方法
WO2013061608A1 (ja) * 2011-10-27 2013-05-02 国立大学法人東京医科歯科大学 大腸上皮幹細胞の単離・培養技術と、これを用いた大腸上皮移植技術
US20140302491A1 (en) * 2011-10-28 2014-10-09 The Board Of Trustees Of The Leland Stanford Junior University Ex Vivo Culture, Proliferation and Expansion of Primary Tissue Organoids
AU2012356133B2 (en) 2011-12-19 2018-07-26 Koninklijke Nederlandse Akademie Van Wetenschappen A rapid quantitative assay to measure CFTR function in a primary intestinal culture model
WO2013131000A1 (en) * 2012-03-01 2013-09-06 Whitehead Institute For Biomedical Research Emt-inducing transcription factors cooperate with sox9
JP6519851B2 (ja) * 2012-07-06 2019-05-29 京都府公立大学法人 眼細胞の分化マーカーおよび分化制御
EP2716298A1 (en) * 2012-10-03 2014-04-09 Institut Pasteur A nod2-dependant pathway of cytoprotection of stem cells
CN104822827B (zh) 2012-12-06 2020-01-21 干细胞生物科技公司 Lgr5+体干细胞
EP2746769A1 (en) 2012-12-21 2014-06-25 Stembios Technologies, Inc. Method for evaluating effect of action on subject based on stem celldynamics
WO2014131033A1 (en) 2013-02-25 2014-08-28 Genentech, Inc. Liquid culturing of epithelial stem cells
BR112015023261A2 (pt) * 2013-03-14 2017-07-18 Massachusetts Inst Technology composições e métodos para expansão e cultura de células-tronco epiteliais
US20160237400A1 (en) * 2013-03-15 2016-08-18 The Jackson Laboratory Isolation of non-embryonic stem cells and uses thereof
TW201522637A (zh) * 2013-03-15 2015-06-16 Jackson Lab 非胚胎幹細胞之單離及其用途
WO2014153294A1 (en) * 2013-03-17 2014-09-25 The Regents Of The University Of California Method to expand and transduce cultured human small and large intestinal stem cells
JP6253265B2 (ja) * 2013-06-05 2017-12-27 学校法人関西医科大学 食道上皮幹細胞の単離方法
JP6437184B2 (ja) * 2013-06-05 2018-12-12 学校法人関西医科大学 舌上皮幹細胞の単離方法
US9595180B2 (en) 2013-08-07 2017-03-14 Nike, Inc. Activity recognition with activity reminders
CA2920856C (en) 2013-08-09 2026-03-03 Ardelyx, Inc. Ph modulating compounds for inhibiting gastrointestinal phosphate uptake
BR102013021202B1 (pt) * 2013-08-20 2022-09-06 Ccb - Centro De Criogenia Brasil Ltda Processo de produção de células tronco multipotentes e progenitores
KR102338815B1 (ko) * 2013-12-16 2021-12-13 프레제니우스 메디칼 케어 도이칠란드 게엠베하 췌장섬-유사 세포 구조물 및 이의 제조 방법
US11390852B2 (en) 2014-01-14 2022-07-19 Yale University Compositions and methods of preparing airway cells
JP2015149949A (ja) * 2014-02-17 2015-08-24 学校法人明治大学 免疫抑制剤の評価方法、及び免疫抑制剤評価キット
WO2015173425A1 (en) 2014-05-16 2015-11-19 Koninklijke Nederlandse Akademie Van Wetenschappen Improved culture method for organoids
ES2860423T3 (es) 2014-05-28 2021-10-05 Childrens Hospital Med Ct Métodos y sistemas para convertir células precursoras en tejidos gástricos mediante diferenciación dirigida
JP2017524377A (ja) 2014-06-20 2017-08-31 ラトガース，ザ ステート ユニバーシティ オブ ニュー ジャージー 単一細胞由来オルガノイド
US20170233698A1 (en) * 2014-06-27 2017-08-17 The Regents Of The University Of California Cultured mammalian limbal stem cells, methods for generating the same, and uses thereof
CN107073042A (zh) 2014-09-03 2017-08-18 布里格海姆妇女医院公司 用于产生内耳毛细胞来治疗听力损失的组合物、系统和方法
JP6796059B2 (ja) * 2014-09-12 2020-12-02 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー Ｗｎｔシグナリングアゴニスト分子
CA2963704A1 (en) 2014-10-17 2016-04-21 Children's Hospital Medical Center In vivo model of human small intestine using pluripotent stem cells and methods of making and using same
GB201421092D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
GB201421094D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
US9777052B2 (en) 2014-12-02 2017-10-03 The Board Of Regents Of The University Of Oklahoma R-spondin variants, compositions, and methods of use
US10100285B2 (en) 2015-04-03 2018-10-16 Propagenix Inc. Ex vivo proliferation of epithelial cells
CN107636149A (zh) 2015-04-03 2018-01-26 普罗帕格尼克斯公司 上皮细胞的离体增殖
JP6662518B2 (ja) * 2015-04-09 2020-03-11 国立大学法人 東京医科歯科大学 腸上皮間リンパ球をインビトロで維持・増殖させる方法
CN104862272B (zh) * 2015-05-29 2018-08-10 吉林大学 Y-27632抑制剂在cd44阳性肠干细胞分选中的应用
WO2017036533A1 (en) * 2015-09-03 2017-03-09 Ecole Polytechnique Federale De Lausanne (Epfl) Three-dimensional hydrogels for culturing adult epithelial stem cells and organoids
JP6869231B2 (ja) 2015-09-11 2021-05-12 プロパジェニクス インコーポレイテッド ｅｘ ｖｉｖｏでの上皮細胞の増殖
WO2017060240A1 (en) 2015-10-05 2017-04-13 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Method and culture medium for ex vivo culturing of epidermis-derived stem cells
CN108291197B (zh) 2015-10-21 2022-09-16 印第安纳大学研究与技术公司 生成人内耳感觉上皮和感觉神经元的方法
JP6376578B2 (ja) * 2016-02-18 2018-08-22 学校法人慶應義塾 細胞培養培地、培養方法、及びオルガノイド
GB201603569D0 (en) 2016-03-01 2016-04-13 Koninklijke Nederlandse Akademie Van Wetenschappen Improved differentiation method
US11753626B2 (en) 2016-03-09 2023-09-12 Beijing Percans Oncology Co., Ltd. Tumor cell suspension cultures and related methods
CN105695394A (zh) * 2016-04-07 2016-06-22 中山大学 一种小鼠唾液腺类器官体的体外培养方法
CN109415685B (zh) 2016-05-05 2023-07-04 儿童医院医疗中心 用于体外制造胃底组织的方法和与其相关的组合物
EP3690022B1 (en) * 2016-05-18 2026-03-18 Keio University Cell culture medium for culturing organoid, culture method, and organoid
CN105950539B (zh) * 2016-05-23 2020-01-07 华东师范大学 人小肠3d类器官研究p-糖蛋白模型的构建方法和应用
GB201610748D0 (en) * 2016-06-20 2016-08-03 Koninklijke Nederlandse Akademie Van Wetenschappen Improved diffrentation method
CN106190980A (zh) * 2016-07-12 2016-12-07 张云霞 一种基于人食管癌组织用于体外培养食管癌肿瘤类器官的专用培养基及培养方法
JP6869554B2 (ja) * 2016-08-24 2021-05-12 学校法人慶應義塾 ヒト下痢症ウイルスの感染・増殖培養用２ｄオルガノイド及びその使用
CA3036378A1 (en) * 2016-09-13 2018-03-22 President And Fellows Of Harvard College Methods relating to intestinal organ-on-a-chip
US11180735B2 (en) 2016-10-28 2021-11-23 The Board Of Trustees Of The Leland Stanford Junior University Methods to preserve tumor-stromal interactions in culture and therapeutic predictive applications thereof
SG10202104575WA (en) * 2016-11-04 2021-06-29 Childrens Hospital Med Ct Liver organoid compositions and methods of making and using same
US20190284536A1 (en) * 2016-11-21 2019-09-19 Beijing Percans Oncology Co., Ltd. Epithelial tumor cell cultures
CA3045145A1 (en) 2016-12-05 2018-06-14 Children's Hospital Medical Center Colonic organoids and methods of making and using same
AU2018211985B2 (en) 2017-01-26 2025-02-13 Surrozen Operating, Inc. Tissue-specific Wnt signal enhancing molecules and uses thereof
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
US12281334B2 (en) 2017-04-14 2025-04-22 Children's Hospital Medical Center Multi donor stem cell compositions and methods of making same
CN107012116A (zh) * 2017-05-09 2017-08-04 华东师范大学 一种小肠3d类器官研究bcrp介导药物转运模型的构建方法与应用
WO2018218344A1 (en) * 2017-05-29 2018-12-06 Stemcell Technologies Canada Inc. Compositions and methods for obtaining organoids
CN109136188A (zh) * 2017-06-15 2019-01-04 上海集技生物技术有限公司 一种活检肠道肿瘤类器官的培养、传代、冻存和复苏方法及其应用
WO2019074793A1 (en) 2017-10-10 2019-04-18 Children's Hospital Medical Center OESOPHAGIAN TISSUE COMPOSITIONS AND / OR ORGANOIDS AND METHODS OF MAKING SAME
CN108118026A (zh) * 2017-11-29 2018-06-05 宁夏医科大学总医院 一种人子宫内膜腺上皮细胞的分离培养方法及优化培养基
KR20200091885A (ko) 2017-11-30 2020-07-31 고쿠리츠 다이가쿠 호진 교토 다이가쿠 세포의 배양 방법
CN107841487A (zh) * 2017-12-13 2018-03-27 章毅 培养味觉干细胞的方法
CN118271444A (zh) 2017-12-19 2024-07-02 瑟罗泽恩奥普瑞汀公司 Wnt替代分子和其用途
CN111699003B (zh) 2017-12-19 2024-05-03 瑟罗泽恩奥普瑞汀公司 抗lrp5/6抗体和使用方法
JP7418332B2 (ja) 2017-12-19 2024-01-19 スロゼン オペレーティング， インコーポレイテッド 抗フリズルド抗体及び使用方法
GB201721615D0 (en) 2017-12-21 2018-02-07 Koninklijke Nederlandse Akademie Van Wetenschappen Immune cell organoid co-cultures
US12379372B2 (en) 2017-12-21 2025-08-05 Children's Hospital Medical Center Digitalized human organoids and methods of using same
EP3530732A1 (en) 2018-02-23 2019-08-28 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Ovarian cancer organoid culture
US12012599B2 (en) 2018-04-13 2024-06-18 Cz Biohub Sf, Llc Compositions and methods for modulating left-right differentiation factor (LEFTY) and bone morphogenic factor (BMP)
CN110452875A (zh) * 2018-05-07 2019-11-15 北京吉尚立德生物科技有限公司 一种用于培养肺癌实体瘤原代细胞的培养基
FR3081168B1 (fr) * 2018-05-21 2022-03-11 Univ Bordeaux Systeme de culture cellulaire en bioreacteur
EP3896154A4 (en) 2018-06-13 2022-03-09 Genex Health Co.,Ltd PRIMARY CELL OF SOLID TUMOR IN COLORECTAL CANCER AND METHODS FOR CULTIVATION OF PRIMARY TUMOR CELL FROM ASCITES IN COLORECTAL CANCER AND RELEVANT REAGENT
CN110592018A (zh) * 2018-06-13 2019-12-20 北京吉尚立德生物科技有限公司 一种结直肠癌实体瘤原代细胞的培养方法
AU2019297522B2 (en) 2018-07-05 2026-04-23 Surrozen Operating, Inc. Multi-specific Wnt surrogate molecules and uses thereof
JP2021530223A (ja) 2018-07-09 2021-11-11 スロゼン， インコーポレイテッド 組織特異性Ｗｎｔシグナル増強分子及びその使用
EP3823661A4 (en) 2018-07-16 2022-05-11 The Board of Trustees of the Leland Stanford Junior University SPECIFIC FRIZZLED WNT AGONISTS AND ANTAGONISTS
CA3106634A1 (en) 2018-07-26 2020-01-30 Children's Hospital Medical Center Hepato-biliary-pancreatic tissues and methods of making same
JP7446224B2 (ja) * 2018-08-08 2024-03-08 中外製薬株式会社 がん組織またはがん組織に類似した組織の培養方法
CN109055304B (zh) * 2018-08-16 2021-12-07 洪玥 一种非柱状上皮干细胞培养基及培养方法
WO2020037326A1 (en) 2018-08-17 2020-02-20 Frequency Therapeutics, Inc. Compositions and methods for generating hair cells by downregulating foxo
CN109207428B (zh) * 2018-09-12 2022-03-22 上海易对医生物医药科技有限公司 循环肿瘤细胞的分离和培养方法
CN112823012A (zh) 2018-09-12 2021-05-18 儿童医院医学中心 用于产生造血干细胞及其衍生物的类器官组合物
ES3037975T3 (en) * 2018-09-26 2025-10-08 Organtech Inc In vitro growth method for hair follicular epithelial stem cells
CN111197024B (zh) * 2018-11-16 2023-08-18 杭州捷诺飞生物科技股份有限公司 类胰腺结构体及其构建方法与应用
GB201819224D0 (en) 2018-11-26 2019-01-09 Koninklijke Nederlandse Akademie Van Wetenschappen Hepatocyte expansion methods
WO2020160371A1 (en) 2019-02-01 2020-08-06 The University Of Hong Kong Innervated organoid compositions and methods of making same
US12221630B2 (en) 2019-02-19 2025-02-11 Miltenyi Biotec B.V. & Co. KG Cell culture medium and method for generation of epithelial organoids from epithelial stem cells
CN109679913A (zh) * 2019-02-26 2019-04-26 复旦大学附属眼耳鼻喉科医院 嗅觉干细胞三维培养方法
GB201903573D0 (en) 2019-03-15 2019-05-01 Koninklijke Nederlandse Akademie Van Wetenschappen Culture method for head and neck organoids
GB201906978D0 (en) 2019-05-17 2019-07-03 Koninklijke Nederlandse Akademie Van Wetenschappen Improved culture method using integrin agonist
US12534709B2 (en) 2019-05-31 2026-01-27 Children's Hospital Medical Center Shaped organoid compositions and methods of making same
CA3141807A1 (en) 2019-05-31 2020-12-03 Children's Hospital Medical Center Methods of generating and expanding hematopoietic stem cells
MX2022000064A (es) 2019-07-03 2022-05-18 Univ Cornell MÉTODOS DE VASCULARIZACIÓN FUNCIONAL DE ISLOTES PANCREÁTICOS Y ORGANOIDES DE CÉLULAS ß.
EP3772538A1 (en) 2019-08-09 2021-02-10 Urosphere Method for differentiating epithelial stem cells
EP3789049A1 (en) 2019-09-06 2021-03-10 QGel SA Method for obtaining healthy intestinal organoids
EP4048809B1 (en) 2019-10-22 2026-05-27 Tempus AI, Inc. Systems and methods for predicting therapeutic sensitivity
US11629385B2 (en) 2019-11-22 2023-04-18 Tempus Labs, Inc. Tumor organoid culture compositions, systems, and methods
WO2021110665A1 (en) 2019-12-04 2021-06-10 Qgel Sa Method and kit for cell growth
JP7744340B2 (ja) 2019-12-05 2025-09-25 テンパス・エーアイ・インコーポレイテッド ハイスループット薬物スクリーニングのためのシステムおよび方法
US12031977B2 (en) 2019-12-10 2024-07-09 Massachusetts Institute Of Technology Ex vivo system for determining multiple drug-drug transporter interactions and methods of use thereof
AU2020399785A1 (en) * 2019-12-12 2022-06-30 The Walter And Eliza Hall Institute Of Medical Research Organoid cultures
US12668773B2 (en) * 2019-12-16 2026-06-30 Jsr Corporation Production method for organoid
US20230056661A1 (en) * 2020-01-07 2023-02-23 Egenesis, Inc. Methods and compositions for production of xenogeneic islet cells and treatment of insulin-resistant or -deficient conditions with the same
GB202004706D0 (en) 2020-03-31 2020-05-13 Koninklijke Nederlandse Akademie Van Wetenschappen Methods and tools for studying enteroendocrine cells
CN113528425B (zh) * 2020-04-15 2023-05-30 合肥中科普瑞昇生物医药科技有限公司 一种用于乳腺上皮肿瘤细胞的培养基和培养方法
US11561178B2 (en) 2020-04-20 2023-01-24 Tempus Labs, Inc. Artificial fluorescent image systems and methods
CN111534477B (zh) * 2020-05-13 2021-12-21 江苏省人民医院（南京医科大学第一附属医院） 小鼠肺组织原代上皮干细胞球培养方法
CN114423858A (zh) * 2020-06-25 2022-04-29 株式会社下一代生物 标准型类器官的生产方法
US12606783B2 (en) 2020-06-25 2026-04-21 Next & Bio Inc. Well plate and 3D culture plate comprising the same
EP4174171A4 (en) 2020-06-25 2024-03-27 Next & Bio Inc. Method for mass proliferation of stem cells without using hydrogel
CN115885037B (zh) * 2020-06-29 2025-11-11 广东省奥干诺伊德生物科技有限公司 中脑类器官及其高速大规模制造方法、利用它筛选神经毒性物质和筛选多巴胺能神经元相关疾病药物的方法
CN114085803B (zh) * 2020-08-24 2024-04-26 北京大学 建立具有再生特性的类器官培养体系
IL302830A (en) 2020-11-13 2023-07-01 Egenesis Inc Cells, tissues, organs and animals with one or more genes that have been modified for improved survival and tolerance of a different-sex transplant
WO2022104280A1 (en) 2020-11-16 2022-05-19 Surrozen Operating, Inc. Liver-specific wnt signal enhancing molecules and uses thereof
CN114763534B (zh) * 2021-01-13 2024-04-26 合肥中科普瑞昇生物医药科技有限公司 原代胃肠间质瘤细胞培养基、培养方法及其应用
WO2022181880A1 (ko) 2021-02-24 2022-09-01 한국화학연구원 인간 전분화능 줄기세포 유래 약물 대사능이 증진된 간 오가노이드의 제조 방법 및 상기 방법으로 제조된 간 오가노이드
KR102746473B1 (ko) 2021-07-09 2024-12-26 주식회사 넥스트앤바이오 가상 오가노이드를 이용한 촬영 이미지 데이터 생성 방법
KR102746470B1 (ko) 2021-07-09 2024-12-26 주식회사 넥스트앤바이오 가상 오가노이드 생성 방법
GB202109913D0 (en) 2021-07-09 2021-08-25 Stichting Hubrecht Organoid Tech Organoid-derived monolayers and uses thereof
CN114134102A (zh) * 2021-11-10 2022-03-04 杭州同创越诚基因科技有限公司 从结肠黏膜组织中分离隐窝并诱导产生结肠类器官的方法
CN114574426A (zh) * 2022-01-25 2022-06-03 湖北省农业科学院畜牧兽医研究所 一种鸭小肠隐窝干细胞分离鉴定和3d类器官培养的方法
GB202204266D0 (en) 2022-03-25 2022-05-11 Stichting Hubrecht Organoid Tech Methods for co-culturing genotoxic bacteria and organoids
WO2023217129A1 (zh) * 2022-05-10 2023-11-16 上海赛立维生物科技有限公司 肝内胆管前体样细胞、细胞制剂及制备方法和应用
CN115322948B (zh) * 2022-07-20 2023-08-29 创芯国际生物科技(广州)有限公司 一种微量原代组织类器官培养前的扩增培养方法
EP4495227A1 (en) 2023-07-18 2025-01-22 HUB Organoids IP B.V. Co-cultures of organoids and stromal cells
EP4549551A1 (en) * 2023-10-31 2025-05-07 Vilnius University The method for the generation of immortalized intestinal epithelial cells
AU2024369604A1 (en) 2023-11-02 2026-05-14 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Method for expanding liver cells
CN117598248A (zh) * 2023-12-26 2024-02-27 华西精准医学产业创新中心有限公司 一种肿瘤药敏动物模型的建模方法及其用途
WO2026099481A1 (en) 2024-11-11 2026-05-15 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Method for producing cells having an acinar cell phenotype
EP4779306A1 (en) 2025-01-17 2026-07-22 HUB Organoids IP B.V. Co-cultures of organoids, stromal cells and immune cells
CN120330136B (zh) * 2025-06-13 2025-10-03 南京兰伯艾克斯生物科技有限公司 一种干细胞克隆培养方法及无血清培养基

Family Cites Families (119)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5464758A (en) 1993-06-14 1995-11-07 Gossen; Manfred Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
DE69523750T2 (de) 1994-05-04 2002-08-01 Mount Sinai Hospital Corp., Toronto Modulatoren der cytokine der tgf-beta überfamilie und verfahren zu ihrer bestimmung
US6043092A (en) 1996-03-18 2000-03-28 University Of Pittsburgh Cell culture media for mammalian cells
US6133027A (en) 1996-08-07 2000-10-17 City Of Hope Inducible expression system
US6207455B1 (en) 1997-05-01 2001-03-27 Lung-Ji Chang Lentiviral vectors
CN1247788C (zh) 1997-05-13 2006-03-29 北卡罗来纳-查佩尔山大学 基于慢病毒的基因转移载体
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
US6218181B1 (en) 1998-03-18 2001-04-17 The Salk Institute For Biological Studies Retroviral packaging cell line
EP0953633A1 (en) 1998-04-28 1999-11-03 Livercell L.L.C. Cell culturing method and medium for producing proliferated, normal, differentiated human liver cells
AU3859800A (en) 1999-02-24 2000-09-14 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Artificial salivary gland
DE19909769A1 (de) 1999-03-05 2000-09-07 Bundesrepublik Deutschland Let Von SIVagm abgeleitete lentivirale Vektoren, Verfahren zu ihrer Herstellung und ihre Verwendung zur Genübertragung in Säugerzellen
FR2794473B1 (fr) 1999-06-03 2003-09-26 Centre Nat Rech Scient Procede de multiplication de cellules souches
EP1224259A4 (en) 1999-09-27 2005-04-27 Univ Florida INVERSION OF INSULIN DEPENDENT DIABETES BY ISOLATED STEM CELLS, PROGENITOR ISLANDIC CELLS, AND INSULAR TYPE STRUCTURES
JP2003530124A (ja) 2000-04-05 2003-10-14 麒麟麦酒株式会社 新規幹細胞成長因子様ポリペプチドおよびポリヌクレオチドに関する方法および物質
US6436704B1 (en) * 2000-04-10 2002-08-20 Raven Biotechnologies, Inc. Human pancreatic epithelial progenitor cells and methods of isolation and use thereof
US20020151487A1 (en) 2000-08-31 2002-10-17 Loyola University Chicago Method and reagents for epithelial barrier formation and treatment of malignant and benign skin disorders by modulating the notch pathway
JP2002247978A (ja) 2001-02-23 2002-09-03 Kazumori Funatsu 肝細胞オルガノイドおよびその製造方法
US20030032034A1 (en) 2001-03-05 2003-02-13 Tang Y. Tom Methods and materials relating to stem cell growth factor-like polypeptides and polynucleotides
US7411052B2 (en) 2001-03-05 2008-08-12 Nuvelo, Inc. Methods and materials relating to stem cell growth factor-like polypeptides and polynucleotides
US20030003088A1 (en) * 2001-05-03 2003-01-02 Board Of Trustees Operating Michigan State University Human pancreatic pluripotential stem cell line
WO2003076564A2 (en) * 2001-05-16 2003-09-18 The General Hospital Corporation Tissue-engineered organs
AU2002331669A1 (en) * 2001-08-23 2003-03-10 The Wistar Institute Of Anatomy And Biology An organotypic intestinal culture and methods of use thereof
EP1427747B1 (en) 2001-08-30 2012-04-11 ARCA biopharma, Inc. Methods and materials relating to stem cell growth factor-like polypeptides and polynucleotides
US20050130145A1 (en) 2001-10-03 2005-06-16 Henry Yue Secreted proteins
US20050053588A1 (en) 2001-10-18 2005-03-10 Li Yin Conversion of liver stem and progenitor cells to pancreatic functional cells
GB2399823B (en) * 2001-12-07 2006-02-15 Geron Corp Islet cells from primate pluripotent stem cells
WO2003054152A2 (en) 2001-12-10 2003-07-03 Nuvelo, Inc. Novel nucleic acids and polypeptides
GB0130738D0 (en) 2001-12-21 2002-02-06 Serono Internat S A Protein
US7193069B2 (en) 2002-03-22 2007-03-20 Research Association For Biotechnology Full-length cDNA
AU2003302702B2 (en) 2002-12-05 2008-08-07 Technion Research & Development Foundation Ltd. Cultured human pancreatic islets, and uses thereof
US20070020637A1 (en) 2003-01-21 2007-01-25 Research Association For Biotechnology Full-length cDNA
EP1440981A3 (en) 2003-01-21 2005-11-23 Research Association for Biotechnology Full-length human cdna
WO2004076642A2 (en) 2003-02-27 2004-09-10 The Rockefeller University Method for modulating epithelial stem cell lineage
WO2004087896A2 (en) 2003-03-31 2004-10-14 Pfizer Products Inc. Hepatocyte differentiation of stem cells
US20040229355A1 (en) 2003-05-14 2004-11-18 Board Of Regents University Of Texas System Culture medium for long-term culture of hepatocytes
US20070010008A1 (en) 2005-06-29 2007-01-11 Tissuetech, Inc. Ex vivo expansion of primary animal cells
EP1629089A1 (en) * 2003-06-03 2006-03-01 Organ Recovery Systems, Inc. Selection and propagation of progenitor cells
WO2005110009A2 (en) 2003-07-22 2005-11-24 Immunex Corporation COMPOSITIONS AND METHODS RELATING TO TSP-30a, b, c AND d
US20050058687A1 (en) 2003-09-12 2005-03-17 Becton, Dickinson And Company Covalently attached collagen VI for cell attachment and proliferation
ATE466106T1 (de) 2003-10-10 2010-05-15 Deutsches Krebsforsch Zusammensetzungen für die diagnose und therapie von erkrankungen, die im zusammenhang mit einer anomalen expression von futrinen (r-spondinen) stehen
WO2005040391A1 (en) 2003-10-27 2005-05-06 Murdoch Childrens Research Institute Compositions and methods for differentiating stem cells
SG149059A1 (en) 2004-01-27 2009-01-29 Nuvelo Inc Gastrointestinal proliferative factor and uses thereof
SE0400974D0 (sv) 2004-04-15 2004-04-15 Cellmax Technologies Ab Dipole design
WO2005113751A1 (en) * 2004-05-14 2005-12-01 Becton, Dickinson And Company Cell culture environments for the serum-free expansion of mesenchymal stem cells
US20070036769A9 (en) * 2004-06-03 2007-02-15 Linheng Li BMP pathway methods and compositions
SE0402734D0 (sv) 2004-11-10 2004-11-10 Anders Grubb Antimiicrobial compounds
US8597947B2 (en) 2004-12-29 2013-12-03 Hadasit Medical Research Services & Development Limited Undifferentiated stem cell culture systems
US7439327B2 (en) 2005-01-18 2008-10-21 Nuvelo, Inc. Stem cell factor-like proteins and uses thereof
US20060182724A1 (en) * 2005-02-15 2006-08-17 Riordan Neil H Method for expansion of stem cells
US8492148B2 (en) 2005-02-23 2013-07-23 Foundation For Biomedical Research & Innovation Method for amplification of endothelial progenitor cell in vitro
JP2006325444A (ja) 2005-05-24 2006-12-07 Toyobo Co Ltd 細胞増殖培地
AU2006202209B2 (en) * 2005-05-27 2011-04-14 Lifescan, Inc. Amniotic fluid derived cells
EP1917022A2 (en) 2005-07-26 2008-05-07 Kirin Pharma Kabushiki Kaisha Anti-tumor agents comprising r-spondins
US7541431B2 (en) 2005-09-07 2009-06-02 Maine Medical Center Cristin/R-spondin ligands active in the Wnt signaling pathway and methods, compositions and kits relating thereto
WO2007030693A2 (en) 2005-09-08 2007-03-15 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Methods for promoting stem cell proliferation and survival
EP1942926A4 (en) 2005-10-07 2010-06-16 Nuvelo Inc STEM CELL FACTOR ARTIFICIAL PROTEIN SCFA1 AND ITS USES
AU2006306809B2 (en) 2005-10-24 2012-07-19 Agency For Science, Technology And Research Methods of specifying mesodermal, endodermal and mesoendodermal cell fates
JP2007116926A (ja) 2005-10-25 2007-05-17 Reprocell Inc 体外における幹細胞の維持と純化に関する方法、組成物およびシステム
EP1792979A1 (en) 2005-12-01 2007-06-06 Stiftung Caesar Center of Advanced European Studies and Research Cell culture system for the enrichment and expansion of stem cells
CA2634510C (en) 2005-12-21 2018-01-09 Universite Catholique De Louvain Isolated liver stem cells
EP2420565B1 (en) * 2006-02-23 2017-08-30 ViaCyte, Inc. Compositions and methods useful for culturing differentiable cells
US8741643B2 (en) * 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
ES2725601T3 (es) * 2006-04-28 2019-09-25 Lifescan Inc Diferenciación de células madre embriónicas humanas
US7989204B2 (en) * 2006-04-28 2011-08-02 Viacyte, Inc. Hepatocyte lineage cells
WO2007149182A2 (en) * 2006-06-19 2007-12-27 Geron Corporation Differentiation and enrichment of islet-like cells from human pluripotent stem cells
JP2008044926A (ja) 2006-08-14 2008-02-28 Trustees Of Columbia Univ In The City Of New York Ｗｎｔ信号伝達に関係した分泌タンパク質
JP2008061569A (ja) 2006-09-07 2008-03-21 Toyobo Co Ltd 幹細胞の培養方法及びその培地
US8628964B2 (en) * 2006-10-11 2014-01-14 Drexel University Fetal pulmonary cells and uses thereof
HUE027179T2 (en) 2006-10-20 2016-10-28 Deutsches Krebsforschungszentrum Stiftung Des Oeffentlichen Rechts Angiogenesis and vasculogenesis modulator for Rspond
RU2323252C1 (ru) 2006-10-25 2008-04-27 Антонина Ивановна Колесникова Способ культивирования мезенхимальных стволовых клеток человека ex vivo
US20080182328A1 (en) 2006-12-19 2008-07-31 The Burnham Institute Mammalian extraembryonic endoderm cells and methods of isolation
WO2008075796A1 (ja) 2006-12-21 2008-06-26 Kyowa Hakko Kirin Co., Ltd. 血球回復促進剤
WO2008088524A2 (en) 2006-12-28 2008-07-24 Nuvelo, Inc. Thrombospondin-domain-deficient r-spondin 1 protein as gastrointestinal tract epithelial proliferation factor
EP2126045A4 (en) 2007-01-30 2010-05-26 Univ Georgia EARLY MESODERM CELLS, STABLE POPULATION OF MESENDODERM CELLS WITH THE ABILITY TO GENERATE ENDODERM AND MESODERM CELL LINES AND MULTIPOTENTIAL MIGRATION CELLS
US20080233088A1 (en) * 2007-02-16 2008-09-25 Varian Medical Systems Technologies, Inc. Preparative regimen for engraftment, growth and differentiation of non-hematopoeitic cells in vivo after transplantation
EP2164512A2 (en) 2007-06-20 2010-03-24 Actogenix N.V. Methods and compositions for treating mucositis
ES2694018T3 (es) 2007-07-02 2018-12-17 Oncomed Pharmaceuticals, Inc. Composiciones y métodos para el tratamiento y el diagnóstico del cáncer
WO2009012428A2 (en) 2007-07-18 2009-01-22 Lifescan, Inc. Differentiation of human embryonic stem cells
EP2022848A1 (en) 2007-08-10 2009-02-11 Hubrecht Institut A method for identifying, expanding, and removing adult stem cells and cancer stem cells
JP2009195142A (ja) 2008-02-20 2009-09-03 Institute Of Physical & Chemical Research 肝臓卵形細胞の取得方法
JP2011522515A (ja) 2008-04-10 2011-08-04 マサチューセッツ インスティテュート オブ テクノロジー 癌幹細胞を標的とする薬剤を同定する方法およびその使用
EP2318520B1 (en) 2008-07-25 2017-10-11 The University Of Georgia Research Foundation, Inc. Compositions for mesoderm derived isl1+ multipotent cells (imps), epicardial progenitor cells (epcs) and multipotent cxcr4+cd56+ cells (c56cs) and methods of use
WO2010016766A2 (en) 2008-08-08 2010-02-11 Koninklijke Nederlandse Akademie Van Wetenschappen Antibodies recognizing endogenous human lgr5 and/or lgr6
US9464275B2 (en) * 2008-08-21 2016-10-11 The Board Of Trustees Of The Leland Stanford Junior University Ex vivo culture, proliferation and expansion of intestinal epithelium
EP2362899A1 (en) 2008-10-31 2011-09-07 Katholieke Universiteit Leuven Optimized methods for differentiation of cells into cells with hepatocyte and hepatocyte progenitor phenotypes, cells produced by the methods, and methods for using the cells
DK2902031T3 (en) 2008-12-08 2019-01-14 Soligenix Inc Topically active steroid for use in radiation damage
SG172160A1 (en) 2008-12-17 2011-07-28 Scripps Research Inst Generation and maintenance of stem cells
US9752124B2 (en) 2009-02-03 2017-09-05 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium for epithelial stem cells and organoids comprising the stem cells
GB201111244D0 (en) 2011-06-30 2011-08-17 Konink Nl Akademie Van Wetenschappen Knaw Culture media for stem cells
EP2412800A1 (en) 2010-07-29 2012-02-01 Koninklijke Nederlandse Akademie van Wetenschappen Liver organoid, uses thereof and culture method for obtaining them
US8642339B2 (en) 2009-02-03 2014-02-04 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium for epithelial stem cells and organoids comprising the stem cells
EP2408466B1 (en) 2009-03-18 2015-10-21 The Brigham and Women's Hospital, Inc. Dikkopf2 (Dkk2) and active fragments thereof for use in the treatment of kidney or pancreas injuries
WO2010108126A2 (en) 2009-03-19 2010-09-23 Fate Therapeutics, Inc. Reprogramming compositions and methods of using the same
US20120039912A1 (en) 2009-04-15 2012-02-16 Galapagos Sasu Rspondin-3 inhibition in bone disorders
WO2010129294A2 (en) 2009-04-27 2010-11-11 Schulz Thomas C Small molecules supporting pluripotent cell growth and methods thereof
US20110002897A1 (en) 2009-06-11 2011-01-06 Burnham Institute For Medical Research Directed differentiation of stem cells
WO2011159726A2 (en) 2010-06-14 2011-12-22 The Scripps Research Institute Reprogramming of cells to a new fate
WO2012044992A2 (en) 2010-09-30 2012-04-05 Agency For Science, Technology And Research (A*Star) Methods and reagents for detection and treatment of esophageal metaplasia
US9732319B2 (en) 2010-12-22 2017-08-15 Fate Therapeutics, Inc. Cell culture platform for single cell sorting and enhanced reprogramming of iPSCs
GB201106395D0 (en) 2011-04-14 2011-06-01 Hubrecht Inst Compounds
CN103619168B (zh) 2011-06-29 2015-09-23 宾州研究基金会 用于治疗白血病的组合物、方法和药盒
US20130052729A1 (en) 2011-08-29 2013-02-28 Olivier Pourquie Method for preparing induced paraxial mesoderm progenitor (ipam) cells and their use
US20130089562A1 (en) 2011-10-05 2013-04-11 Genenthech, Inc. Methods of treating liver conditions using notch2 antagonists
GB201117469D0 (en) 2011-10-10 2011-11-23 Cell Guidance Systems Ltd Culture media for pluripotent stem cells
WO2013061608A1 (ja) 2011-10-27 2013-05-02 国立大学法人東京医科歯科大学 大腸上皮幹細胞の単離・培養技術と、これを用いた大腸上皮移植技術
AU2012356133B2 (en) 2011-12-19 2018-07-26 Koninklijke Nederlandse Akademie Van Wetenschappen A rapid quantitative assay to measure CFTR function in a primary intestinal culture model
WO2014066649A1 (en) 2012-10-26 2014-05-01 The Regents Of The University Of California A strategy for engineering various 3d tissues, organoids and vasculature
US10913933B2 (en) 2013-02-13 2021-02-09 Wake Forest University Health Sciences Bioengineered liver constructs and methods relating thereto
US20150368616A1 (en) 2013-02-14 2015-12-24 The Cleveland Clinic Foundation Methods for induction of cell fates from pluripotent cells
CN105121632B (zh) 2013-02-18 2021-08-10 大学健康网络 由多能干细胞生成肝细胞和胆管细胞的方法
BR112015023261A2 (pt) 2013-03-14 2017-07-18 Massachusetts Inst Technology composições e métodos para expansão e cultura de células-tronco epiteliais
KR20230148263A (ko) 2013-03-15 2023-10-24 더 보드 오브 리젠츠 오브 더 유니버시티 오브 텍사스 시스템 누트린-3a 및 펩티드에 의한 폐 섬유증의 저해
AU2014255755B2 (en) 2013-04-16 2018-10-25 Orbsen Therapeutics Limited Medical use of syndecan-2
WO2015173425A1 (en) 2014-05-16 2015-11-19 Koninklijke Nederlandse Akademie Van Wetenschappen Improved culture method for organoids
CN122465832A (zh) 2014-07-30 2026-07-28 耶达研究及发展有限公司 用于培养多能干细胞的培养基
CN107075471A (zh) 2014-10-08 2017-08-18 新加坡科技研究局 将干细胞分化为肝细胞谱系的方法
GB201421092D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
GB201421094D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
EP3690022B1 (en) 2016-05-18 2026-03-18 Keio University Cell culture medium for culturing organoid, culture method, and organoid

Also Published As

Publication number Publication date
DK3061808T3 (da) 2020-09-07
AU2010211428B2 (en) 2015-09-03
HUE062459T2 (hu) 2023-11-28
EP3441458B1 (en) 2023-06-07
US20120028355A1 (en) 2012-02-02
EP3441458A1 (en) 2019-02-13
KR101904224B1 (ko) 2018-10-04
EP2393917A2 (en) 2011-12-14
EP3441458C0 (en) 2023-06-07
SG173492A1 (en) 2011-09-29
MX379945B (es) 2025-03-11
JP5458112B2 (ja) 2014-04-02
RU2555545C2 (ru) 2015-07-10
JP5722835B2 (ja) 2015-05-27
ES2948761T3 (es) 2023-09-18
HK1163746A1 (zh) 2012-09-14
EP3441458B9 (en) 2023-08-23
WO2010090513A2 (en) 2010-08-12
AU2010211428A1 (en) 2011-08-18
US8642339B2 (en) 2014-02-04
RU2011136704A (ru) 2013-03-10
EP4253529A2 (en) 2023-10-04
CN102439135A (zh) 2012-05-02
CA2751332A1 (en) 2010-08-12
NZ594271A (en) 2014-02-28
EP3061808B1 (en) 2020-08-12
PL3061808T3 (pl) 2021-02-08
EP4253529A3 (en) 2024-01-17
US10947510B2 (en) 2021-03-16
MX2019006565A (es) 2019-08-22
HRP20160791T1 (hr) 2016-09-23
CN102439135B (zh) 2014-04-30
EP3061808A3 (en) 2016-10-19
MX381730B (es) 2025-03-13
ES2948761T9 (es) 2023-11-23
WO2010090513A3 (en) 2010-11-25
US20190100728A1 (en) 2019-04-04
US20140256037A1 (en) 2014-09-11
EP2393917B1 (en) 2016-04-06
PL3441458T3 (pl) 2023-11-06
ES2579909T3 (es) 2016-08-17
JP2012516685A (ja) 2012-07-26
KR20120003855A (ko) 2012-01-11
DK2393917T3 (en) 2016-04-18
CA2751332C (en) 2024-04-02
HUE028647T2 (en) 2016-12-28
JP2012254081A (ja) 2012-12-27
IL214381A (en) 2016-09-29
EP3061808A2 (en) 2016-08-31
IL214381A0 (en) 2011-09-27
HRP20230650T1 (hr) 2023-09-29
MX2011008044A (es) 2012-01-12

Similar Documents

Publication Publication Date Title
IL214381A0 (en) Culture medium for epithelial stem cells and organoids comprising said stem cells
PL2459702T3 (pl) Pożywka do hodowli komórkowej do ekspresji białka adamts
ZA201606403B (en) Pluripotent stem cell culture on micro-carriers
PL2046946T3 (pl) Hodowla pluripotencjalnych komórek macierzystych
GB2446525B (en) Stem cell culture medium and method
GB2458863B (en) Improved culture of stem cells
IL228818A0 (en) Stem cell cultures
EP2142641A4 (en) UNDIFFERENCED STEM CELL CULTURAL SYSTEMS
IL216902A0 (en) Methods for culturing stem and progentitor cells
GB2460552B (en) Stem cell culture media and methods
EP2155859A4 (en) FORMULATIONS AND METHODS FOR CULTIVATING EMBRYONIC STEM CELLS
ZA201000715B (en) Single pluripotent stem cell culture
SG10201602507UA (en) Cell culture medium and process for protein expression, said medium and process comprising a pam inhibitor
ZA200901914B (en) Stem cell culture medium and method
EP2480655B8 (en) Cell culture medium
PT3061808T (pt) Meio de cultura para células estaminais epiteliais e organóides compreeendendo as referidas células estaminais
HUE050984T2 (hu) Tenyészközeg epiteliális õssejtekhez és az õssejteket tartalmazó organoidokhoz
AU2014201623B2 (en) Pluripotent stem cell culture
GB0810304D0 (en) Stem cell culture media and methods
GB0821363D0 (en) Stem cell culture media and methods
AU2009901852A0 (en) Media and methods for cell culture
GB0901069D0 (en) Stem cell culture methods
GB0916476D0 (en) Stem cell culture methods
IL201166A0 (en) Undifferentiated stem cell culture systems
GB0808778D0 (en) Cell culture technique