JPH09505728A - フラボバクテリウム・オケアノコイテス(foki)制限エンドヌクレアーゼにおける機能ドメイン - Google Patents
フラボバクテリウム・オケアノコイテス(foki)制限エンドヌクレアーゼにおける機能ドメインInfo
- Publication number
- JPH09505728A JPH09505728A JP7510290A JP51029095A JPH09505728A JP H09505728 A JPH09505728 A JP H09505728A JP 7510290 A JP7510290 A JP 7510290A JP 51029095 A JP51029095 A JP 51029095A JP H09505728 A JPH09505728 A JP H09505728A
- Authority
- JP
- Japan
- Prior art keywords
- endonuclease
- dna
- dna segment
- foki
- domain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 108091008146 restriction endonucleases Proteins 0.000 title claims abstract description 74
- 241000589565 Flavobacterium Species 0.000 title description 3
- 238000003776 cleavage reaction Methods 0.000 claims abstract description 99
- 230000007017 scission Effects 0.000 claims abstract description 95
- 102000004190 Enzymes Human genes 0.000 claims abstract description 86
- 108090000790 Enzymes Proteins 0.000 claims abstract description 86
- 108010026638 endodeoxyribonuclease FokI Proteins 0.000 claims abstract description 26
- 102000009331 Homeodomain Proteins Human genes 0.000 claims abstract description 22
- 108010048671 Homeodomain Proteins Proteins 0.000 claims abstract description 22
- 101710163270 Nuclease Proteins 0.000 claims abstract description 16
- 108020004414 DNA Proteins 0.000 claims description 243
- 108010042407 Endonucleases Proteins 0.000 claims description 103
- 108090000623 proteins and genes Proteins 0.000 claims description 103
- 102000004533 Endonucleases Human genes 0.000 claims description 102
- 102000004169 proteins and genes Human genes 0.000 claims description 79
- 108020004705 Codon Proteins 0.000 claims description 54
- 230000000694 effects Effects 0.000 claims description 39
- 230000003197 catalytic effect Effects 0.000 claims description 26
- 239000013598 vector Substances 0.000 claims description 26
- 230000004568 DNA-binding Effects 0.000 claims description 20
- 125000003729 nucleotide group Chemical group 0.000 claims description 20
- 239000002773 nucleotide Substances 0.000 claims description 19
- 210000001236 prokaryotic cell Anatomy 0.000 claims description 19
- 150000001413 amino acids Chemical class 0.000 claims description 11
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 8
- 239000011701 zinc Substances 0.000 claims description 8
- 229910052725 zinc Inorganic materials 0.000 claims description 8
- 102000023888 sequence-specific DNA binding proteins Human genes 0.000 claims description 7
- 108091008420 sequence-specific DNA binding proteins Proteins 0.000 claims description 7
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims description 6
- 108700020796 Oncogene Proteins 0.000 claims description 5
- 238000013518 transcription Methods 0.000 claims description 5
- 230000035897 transcription Effects 0.000 claims description 5
- 238000003780 insertion Methods 0.000 abstract description 14
- 230000037431 insertion Effects 0.000 abstract description 14
- 238000010276 construction Methods 0.000 abstract description 12
- 210000004898 n-terminal fragment Anatomy 0.000 abstract description 9
- 210000004900 c-terminal fragment Anatomy 0.000 abstract description 8
- 239000012634 fragment Substances 0.000 description 85
- 239000000758 substrate Substances 0.000 description 64
- 239000012588 trypsin Substances 0.000 description 39
- 102000004142 Trypsin Human genes 0.000 description 38
- 108090000631 Trypsin Proteins 0.000 description 38
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 36
- 230000029087 digestion Effects 0.000 description 32
- 238000003752 polymerase chain reaction Methods 0.000 description 27
- 210000004027 cell Anatomy 0.000 description 26
- 238000004458 analytical method Methods 0.000 description 25
- 239000013612 plasmid Substances 0.000 description 25
- 239000013615 primer Substances 0.000 description 24
- 239000000499 gel Substances 0.000 description 23
- 108091034117 Oligonucleotide Proteins 0.000 description 21
- 239000000047 product Substances 0.000 description 21
- 238000006243 chemical reaction Methods 0.000 description 20
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 18
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L magnesium chloride Substances [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 18
- 239000011780 sodium chloride Substances 0.000 description 18
- 238000000034 method Methods 0.000 description 17
- 229910001629 magnesium chloride Inorganic materials 0.000 description 14
- 241000588724 Escherichia coli Species 0.000 description 13
- 239000000539 dimer Substances 0.000 description 13
- 230000027455 binding Effects 0.000 description 12
- 238000009739 binding Methods 0.000 description 12
- 239000001913 cellulose Substances 0.000 description 12
- 229920002678 cellulose Polymers 0.000 description 12
- 101150057450 fokIR gene Proteins 0.000 description 12
- 239000011541 reaction mixture Substances 0.000 description 12
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 11
- 238000012163 sequencing technique Methods 0.000 description 11
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 10
- 238000001502 gel electrophoresis Methods 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 229920002401 polyacrylamide Polymers 0.000 description 9
- 238000000746 purification Methods 0.000 description 9
- 102000052510 DNA-Binding Proteins Human genes 0.000 description 8
- 108020004511 Recombinant DNA Proteins 0.000 description 8
- 239000000872 buffer Substances 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- 101100024586 Planomicrobium okeanokoites fokIM gene Proteins 0.000 description 7
- 239000007983 Tris buffer Substances 0.000 description 7
- 238000000246 agarose gel electrophoresis Methods 0.000 description 7
- 210000004899 c-terminal region Anatomy 0.000 description 7
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 7
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 7
- 239000000523 sample Substances 0.000 description 7
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 108091028043 Nucleic acid sequence Proteins 0.000 description 6
- 230000000717 retained effect Effects 0.000 description 6
- 101710096438 DNA-binding protein Proteins 0.000 description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
- 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 5
- 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 5
- 230000000295 complement effect Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 101150070061 fokIM gene Proteins 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 230000035772 mutation Effects 0.000 description 5
- 229940080469 phosphocellulose Drugs 0.000 description 5
- 241000894007 species Species 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 102000053602 DNA Human genes 0.000 description 4
- 101100312863 Planomicrobium okeanokoites fokIR gene Proteins 0.000 description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 4
- 125000003275 alpha amino acid group Chemical group 0.000 description 4
- 239000000287 crude extract Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000013604 expression vector Substances 0.000 description 4
- YMTINGFKWWXKFG-UHFFFAOYSA-N fenofibrate Chemical compound C1=CC(OC(C)(C)C(=O)OC(C)C)=CC=C1C(=O)C1=CC=C(Cl)C=C1 YMTINGFKWWXKFG-UHFFFAOYSA-N 0.000 description 4
- 230000014509 gene expression Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 3
- 239000011543 agarose gel Substances 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 238000002523 gelfiltration Methods 0.000 description 3
- 238000013507 mapping Methods 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 2
- 108700020911 DNA-Binding Proteins Proteins 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 2
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 229920005654 Sephadex Polymers 0.000 description 2
- 239000012507 Sephadex™ Substances 0.000 description 2
- 239000007984 Tris EDTA buffer Substances 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 238000000376 autoradiography Methods 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 230000005782 double-strand break Effects 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000012160 loading buffer Substances 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 231100000219 mutagenic Toxicity 0.000 description 2
- 230000003505 mutagenic effect Effects 0.000 description 2
- 108020001580 protein domains Proteins 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 238000004007 reversed phase HPLC Methods 0.000 description 2
- 102220219006 rs1060501597 Human genes 0.000 description 2
- 150000003839 salts Chemical group 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- OSBLTNPMIGYQGY-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid Chemical compound OB(O)O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O OSBLTNPMIGYQGY-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 108010087765 Antipain Proteins 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000193388 Bacillus thuringiensis Species 0.000 description 1
- 108020000946 Bacterial DNA Proteins 0.000 description 1
- 101100539485 Caenorhabditis elegans unc-86 gene Proteins 0.000 description 1
- 208000037088 Chromosome Breakage Diseases 0.000 description 1
- 206010010071 Coma Diseases 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 239000004971 Cross linker Substances 0.000 description 1
- 101710151559 Crystal protein Proteins 0.000 description 1
- 102000013381 DNA Modification Methylases Human genes 0.000 description 1
- 108010090738 DNA Modification Methylases Proteins 0.000 description 1
- 108010044289 DNA Restriction-Modification Enzymes Proteins 0.000 description 1
- 102000006465 DNA Restriction-Modification Enzymes Human genes 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 1
- 108010008532 Deoxyribonuclease I Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 241001524679 Escherichia virus M13 Species 0.000 description 1
- 244000300477 Gardenia carinata Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 206010064571 Gene mutation Diseases 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 108010054278 Lac Repressors Proteins 0.000 description 1
- 235000010689 Lufa Nutrition 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 241001024304 Mino Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 101150054854 POU1F1 gene Proteins 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 241000589579 Planomicrobium okeanokoites Species 0.000 description 1
- 101000801943 Pyrobaculum calidifontis (strain DSM 21063 / JCM 11548 / VA1) Thiamine-monophosphate kinase Proteins 0.000 description 1
- 101100138712 Schizosaccharomyces pombe (strain 972 / ATCC 24843) puc1 gene Proteins 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 102000012479 Serine Proteases Human genes 0.000 description 1
- 108010022999 Serine Proteases Proteins 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 241000194023 Streptococcus sanguinis Species 0.000 description 1
- 239000008051 TBE buffer Substances 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 108010064978 Type II Site-Specific Deoxyribonucleases Proteins 0.000 description 1
- 238000006023 Wilson reaction Methods 0.000 description 1
- ZKHQWZAMYRWXGA-KNYAHOBESA-N [[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] dihydroxyphosphoryl hydrogen phosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)O[32P](O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KNYAHOBESA-N 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- OFHCOWSQAMBJIW-AVJTYSNKSA-N alfacalcidol Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C\C=C1\C[C@@H](O)C[C@H](O)C1=C OFHCOWSQAMBJIW-AVJTYSNKSA-N 0.000 description 1
- ILRRQNADMUWWFW-UHFFFAOYSA-K aluminium phosphate Chemical compound O1[Al]2OP1(=O)O2 ILRRQNADMUWWFW-UHFFFAOYSA-K 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- BSJGASKRWFKGMV-UHFFFAOYSA-L ammonia dichloroplatinum(2+) Chemical compound N.N.Cl[Pt+2]Cl BSJGASKRWFKGMV-UHFFFAOYSA-L 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- SDNYTAYICBFYFH-TUFLPTIASA-N antipain Chemical compound NC(N)=NCCC[C@@H](C=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 SDNYTAYICBFYFH-TUFLPTIASA-N 0.000 description 1
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 description 1
- 229940097012 bacillus thuringiensis Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000009920 chelation Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 210000000078 claw Anatomy 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- BUACSMWVFUNQET-UHFFFAOYSA-H dialuminum;trisulfate;hydrate Chemical compound O.[Al+3].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O BUACSMWVFUNQET-UHFFFAOYSA-H 0.000 description 1
- 238000005363 electrowinning Methods 0.000 description 1
- 238000009585 enzyme analysis Methods 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000005227 gel permeation chromatography Methods 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000010039 intracellular degradation Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000006911 nucleation Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 231100000590 oncogenic Toxicity 0.000 description 1
- 230000002246 oncogenic effect Effects 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 108010083127 phage repressor proteins Proteins 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 238000000734 protein sequencing Methods 0.000 description 1
- 125000000561 purinyl group Chemical group N1=C(N=C2N=CNC2=C1)* 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 238000007480 sanger sequencing Methods 0.000 description 1
- 101150056399 slc20a1 gene Proteins 0.000 description 1
- 239000003998 snake venom Substances 0.000 description 1
- 108010062513 snake venom phosphodiesterase I Proteins 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 239000002753 trypsin inhibitor Substances 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 239000007160 ty medium Substances 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.IIS型エンドヌクレアーゼの認識ドメインをコードする単離されたDN Aセグメントであって、前記IIS型エンドヌクレアーゼの配列特異的認識活性 を含むDNAセグメント。 2.請求項1に記載のDNAセグメントであって、前記IIS型エンドヌクレ アーゼがFokI制限エンドヌクレアーゼであるDNAセグメント。 3.請求項2に記載のDNAセグメントであって、前記コードされるタンパク が、SDSポリアクリルアミドゲル電気泳動により測定したときに約41キロド ルトンの分子量を有するDNAセグメント。 4.請求項3に記載のDNAセグメントであって、FokI制限エンドヌクレ アーゼのアミノ酸1〜382をコードするDNAセグメント。 5.IIS型エンドヌクレアーゼの触媒ドメインをコードする単離されたDN Aセグメントであって、前記IIS型エンドヌクレアーゼの開裂活性を含むDN Aセグメント。 6.請求項5に記載のDNAセグメントであって、前記IIS型エンドヌクレ アーゼがFokI制限エンドヌクレアーゼであるDNAセグメント。 7.請求項6に記載のDNAセグメントであって、前記コードされるタンパク が、SDSポリアクリルアミドゲル電気泳動により測定したときに約25キロド ルトンの分子量を有するDNAセグメント。 8.請求項7に記載のDNAセグメントであって、FokIエンドヌクレアー ゼのアミノ酸383〜578をコードするDNAセグメント。 9.実質的にFokI制限エンドヌクレアーゼのN末端からなる単離されたタ ンパクであって、前記エンドヌクレアーゼの配列特異的認識活性を有するタンパ ク。 10.請求項9に記載のタンパクであって、FokI制限エンドヌクレアーゼ のアミノ酸1〜382であるタンパク。 11.実質的にFokI制限エンドヌクレアーゼのC末端からなる単離された タンパクであって、前記エンドヌクレアーゼの開裂活性を有するタンパク。 12.請求項11に記載のタンパクであって、FokI制限エンドヌクレアー ゼのアミノ酸383〜578であるタンパク。 13.DNA構築物であって: (i)IIS型エンドヌクレアーゼの開裂活性を含むIIS型エンドヌクレ アーゼの触媒ドメインをコードする第一のDNAセグメントと; (ii)前記IIS型エンドヌクレアーゼの認識ドメイン以外の配列特異的認 識ドメインをコードする第二のDNAセグメントと; (iii)ベクターとを具備し、 前記第一のDNAセグメントおよび前記第二のDNAセグメントは、単一のタ ンパクが産生されるように、前記ベクターに対して動作可能に連結されているD NA構築物。 14.請求項13に記載のDNA構築物であって、前記IIS型エンドヌクレ アーゼがFokI制限エンドヌクレアーゼであるDNA構築物。 15.請求項14に記載のDNA構築物であって、前記認識ドメインが、ジン クフィンガーモチーフ;ホメオドメインモチーフ;リプレッサのDNA結合性ド メイン;POUドメインモチーフ(真核転写レギュレータ);発癌遺伝子のDN A結合性ドメイン;および>6塩基対を認識する他の天然に存在する配列特異的 なDNA結合性タンパクからなる群から選択されるDNA構築物。 16.請求項15に記載のDNA構築物であって、前記認識ドメインがUbx のホメオドメインであるDNA構築物。 17.原核細胞であって: (i)IIS型エンドヌクレアーゼの開裂活性を含むIIS型エンドヌクレ アーゼの触媒ドメインをコードする第一のDNAセグメントと; (ii)前記IIS型エンドヌクレアーゼの認識ドメイン以外の配列特異的認 識ドメインをコードする第二のDNAセグメントと; (iii)ベクターとを含んでおり、 前記第一のDNAセグメントおよび前記第二のDNAセグメントは、単一のタ ンパクが産生されるように、前記ベクターに対して動作可能に連結されているD NA構築物。 18.請求項17に記載の原核細胞であって、前記第一のDNAセグメントが FokIの触媒ドメイン(FN)をコー ドし、前記第二のDNAセグメントがUbxのホメオドメインをコードする原核 細胞。 19.IIS型エンドヌクレアーゼの触媒ドメインを含むハイブリッド制限酵 素であって、前記IIS型エンドヌクレアーゼ以外の酵素の認識ドメインに対し て共有結合的に結合された前記IIS型エンドヌクレアーゼの開裂活性を含んだ ハイブリッド制限酵素。 20.請求項19に記載のハイブリッド制限酵素であって、前記認識ドメイン (前記ハイブリッド制限酵素の一部なす)が、ジンクフィンガーモチーフ;ホメ オドメインモチーフ;POUドメインモチーフ;リプレッサのDNA結合性ドメ イン;発癌遺伝子のDNA結合性ドメイン;および>6塩基対を認識する他の天 然に存在する配列特異的なDNA結合性タンパクからなる群から選択されるハイ ブリッド制限酵素。 21.請求項20に記載のハイブリッド制限酵素であって、前記認識ドメイン がUbxのホメオドメインであるハイブリッド制限酵素。 22.請求項21に記載のハイブリッド制限酵素であって、前記II型エンド ヌクレアーゼがFokI制限エンドヌクレアーゼであり、前記ハイブリッド酵素 がUbx−FNであるハイブリッド制限酵素。 23. DNA構築物であって: (i)IIS型エンドヌクレアーゼの開裂活性を含んだIIS型エンドヌク レアーゼの触媒ドメインをコードする第一のDNAセグメントと; (ii)前記IIS型エンドヌクレアーゼの認識ドメイン以外の配列特異的認 識ドメインをコードする第二のDNAセグメントと; (iii)前記第一のDNAセグメントおよび前記第二のDNAセグメントの 間に挿入された、1以上のコドンを含む第三のDNAセグメントと; (iv)ベクターとを含み、 前記第一のDNAセグメント、前記第二のDNAセグメントおよび前記第三の DNAセグメントは、単一のタンパクが産生されるように、前記ベクターに対し て動作可能に連結されているDNA構築物。 24.請求項23に記載のDNA構築物であって、前記IIS型エンドヌクレ アーゼがFokI制限エンドヌクレアーゼであるDNA構築物。 25.請求項24に記載のDNA構築物であって、前記第三のDNAセグメン トが実質的に4コドンからなるDNA構築物。 26.請求項25に記載のDNA構築物であって、前記第三のDNAセグメン トの前記4コドンが、前記エンドヌクレアーゼをコードする遺伝子のヌクレオチ ド1152に挿入されているDNA構築物。 27.請求項24に記載のDNA構築物であって、前記第三のDNAセグメン トが実質的に7コドンからなるDNAセグメント。 28.請求項24に記載のDNA構築物であって、前記認 識ドメインが、ジンクフィンガーモチーフ;ホメオドメインモチーフ;POUド メインモチーフ;リプレッサのDNA結合性ドメイン;発癌遺伝子のDNA結合 性ドメイン;および>6塩基対を認識する他の天然に存在する配列特異的なDN A結合性タンパクからなる群から選択されるDNA構築物。 30.原核細胞であって: (i)IIS型エンドヌクレアーゼの開裂活性を含んだIIS型エンドヌク レアーゼの触媒ドメインをコードする第一のDNAセグメントと; (ii)前記IIS型エンドヌクレアーゼの認識ドメイン以外の配列特異的認 識ドメインをコードする第二のDNAセグメントと; (iii)前記第一のDNAセグメントおよび前記第二のDNAセグメントの 間に挿入された、1以上のコドンを含む第三のDNAセグメントと; (iv)ベクターとを含み、 前記第一のDNAセグメント、前記第二のDNAセグメントおよび前記第三の DNAセグメントは、単一のタンパクが産生されるように、前記ベクターに対し て動作可能に連結されているDNA構築物。 31.請求項30に記載の原核細胞であって、前記第三のDNAセグメントが 実質的に4コドンからなる細胞。 32.請求項30に記載の原核細胞であって、前記第三のDNAセグメントが 実質的に7コドンからなる細胞。 33.請求項30の原核細胞によって産生された、単離さ れたIIS型ハイブリッドエンドヌクレアーゼ。 34.IIS型エンドヌクレアーゼのN末端(該II型エンドヌクレアーゼの 配列特異的認識活性を含む)をコードする単離されたDNAセグメントであって 、前記II型エンドヌクレアーゼはFokI制限エンドヌクレアーゼであり、且 つ前記N末端はSDS−ポリアクリルアミドゲル電気泳動で測定したときに約4 1キロドルトンの分子量を有するDNAセグメント。 35.IIS型エンドヌクレアーゼのC末端(該II型エンドヌクレアーゼの 開裂活性を含む)をコードする単離されたDNAセグメントであって、前記II 型エンドヌクレアーゼはFokI制限エンドヌクレアーゼであり、且つ前記C末 端はSDS−ポリアクリルアミドゲル電気泳動で測定したときに約25キロドル トンの分子量を有するDNAセグメント。 36.実質的にFokI制限エンドヌクレアーゼのN末端からなる単離された タンパクであって、該タンパクは前記エンドヌクレアーゼの配列特異的認識活性 を有し、且つ該タンパクはFokI制限エンドヌクレアーゼのアミノ酸1〜38 2であるタンパク。 37.実質的にFokI制限エンドヌクレアーゼのC末端からなる単離された タンパクであって、該タンパクは前記エンドヌクレアーゼのヌクレアーゼ活性を 有し、且つ該タンパクはFokI制限エンドヌクレアーゼのアミノ酸383〜5 87であるタンパク。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/126,564 | 1993-09-27 | ||
US08/126,564 US5436150A (en) | 1992-04-03 | 1993-09-27 | Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease |
PCT/US1994/009143 WO1995009233A1 (en) | 1993-09-27 | 1994-08-23 | Functional domains in flavobacterium okeanokoites (foki) restriction endonuclease |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006143294A Division JP4081119B2 (ja) | 1993-09-27 | 2006-05-23 | フラボバクテリウム・オケアノコイテス(foki)制限エンドヌクレアーゼにおける機能ドメイン |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH09505728A true JPH09505728A (ja) | 1997-06-10 |
Family
ID=22425524
Family Applications (6)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP7510290A Withdrawn JPH09505728A (ja) | 1993-09-27 | 1994-08-23 | フラボバクテリウム・オケアノコイテス(foki)制限エンドヌクレアーゼにおける機能ドメイン |
JP2006143294A Expired - Lifetime JP4081119B2 (ja) | 1993-09-27 | 2006-05-23 | フラボバクテリウム・オケアノコイテス(foki)制限エンドヌクレアーゼにおける機能ドメイン |
JP2007230093A Withdrawn JP2008005851A (ja) | 1993-09-27 | 2007-09-05 | フラボバクテリウム・オケアノコイテス(foki)制限エンドヌクレアーゼにおける機能ドメイン |
JP2008291366A Withdrawn JP2009072201A (ja) | 1993-09-27 | 2008-11-13 | フラボバクテリウム・オケアノコイテス(foki)制限エンドヌクレアーゼにおける機能ドメイン |
JP2012037730A Withdrawn JP2012135316A (ja) | 1993-09-27 | 2012-02-23 | フラボバクテリウム・オケアノコイテス(foki)制限エンドヌクレアーゼにおける機能ドメイン |
JP2012279894A Pending JP2013081471A (ja) | 1993-09-27 | 2012-12-21 | フラボバクテリウム・オケアノコイテス(foki)制限エンドヌクレアーゼにおける機能ドメイン |
Family Applications After (5)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006143294A Expired - Lifetime JP4081119B2 (ja) | 1993-09-27 | 2006-05-23 | フラボバクテリウム・オケアノコイテス(foki)制限エンドヌクレアーゼにおける機能ドメイン |
JP2007230093A Withdrawn JP2008005851A (ja) | 1993-09-27 | 2007-09-05 | フラボバクテリウム・オケアノコイテス(foki)制限エンドヌクレアーゼにおける機能ドメイン |
JP2008291366A Withdrawn JP2009072201A (ja) | 1993-09-27 | 2008-11-13 | フラボバクテリウム・オケアノコイテス(foki)制限エンドヌクレアーゼにおける機能ドメイン |
JP2012037730A Withdrawn JP2012135316A (ja) | 1993-09-27 | 2012-02-23 | フラボバクテリウム・オケアノコイテス(foki)制限エンドヌクレアーゼにおける機能ドメイン |
JP2012279894A Pending JP2013081471A (ja) | 1993-09-27 | 2012-12-21 | フラボバクテリウム・オケアノコイテス(foki)制限エンドヌクレアーゼにおける機能ドメイン |
Country Status (6)
Country | Link |
---|---|
US (1) | US5436150A (ja) |
EP (1) | EP0721502A4 (ja) |
JP (6) | JPH09505728A (ja) |
AU (1) | AU687435B2 (ja) |
CA (1) | CA2170986A1 (ja) |
WO (1) | WO1995009233A1 (ja) |
Families Citing this family (367)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5916794A (en) * | 1992-04-03 | 1999-06-29 | Johns Hopkins University | Methods for inactivating target DNA and for detecting conformational change in a nucleic acid |
US5436150A (en) * | 1992-04-03 | 1995-07-25 | The Johns Hopkins University | Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease |
US20050084885A1 (en) * | 1994-01-18 | 2005-04-21 | The Scripps Research Institute | Zinc finger protein derivatives and methods therefor |
US6140466A (en) | 1994-01-18 | 2000-10-31 | The Scripps Research Institute | Zinc finger protein derivatives and methods therefor |
GB9824544D0 (en) | 1998-11-09 | 1999-01-06 | Medical Res Council | Screening system |
USRE45721E1 (en) | 1994-08-20 | 2015-10-06 | Gendaq, Ltd. | Relating to binding proteins for recognition of DNA |
JP3817739B2 (ja) * | 1994-12-29 | 2006-09-06 | マサチューセッツ・インスティテュート・オブ・テクノロジー | キメラdna結合性タンパク質 |
AU6096296A (en) * | 1995-06-07 | 1996-12-30 | Ohio State University, The | Artificial restriction endonuclease |
US6261797B1 (en) | 1996-01-29 | 2001-07-17 | Stratagene | Primer-mediated polynucleotide synthesis and manipulation techniques |
GB9710809D0 (en) | 1997-05-23 | 1997-07-23 | Medical Res Council | Nucleic acid binding proteins |
US6444421B1 (en) | 1997-11-19 | 2002-09-03 | The United States Of America As Represented By The Department Of Health And Human Services | Methods for detecting intermolecular interactions in vivo and in vitro |
US6410248B1 (en) | 1998-01-30 | 2002-06-25 | Massachusetts Institute Of Technology | General strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites |
CA2321938C (en) * | 1998-03-02 | 2009-11-24 | Massachusetts Institute Of Technology | Poly zinc finger proteins with improved linkers |
WO1999047656A2 (en) | 1998-03-17 | 1999-09-23 | Gendaq Limited | Nucleic acid binding proteins |
US6534261B1 (en) | 1999-01-12 | 2003-03-18 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
US7070934B2 (en) | 1999-01-12 | 2006-07-04 | Sangamo Biosciences, Inc. | Ligand-controlled regulation of endogenous gene expression |
US6453242B1 (en) * | 1999-01-12 | 2002-09-17 | Sangamo Biosciences, Inc. | Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites |
US7013219B2 (en) | 1999-01-12 | 2006-03-14 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
US6599692B1 (en) * | 1999-09-14 | 2003-07-29 | Sangamo Bioscience, Inc. | Functional genomics using zinc finger proteins |
US6503717B2 (en) * | 1999-12-06 | 2003-01-07 | Sangamo Biosciences, Inc. | Methods of using randomized libraries of zinc finger proteins for the identification of gene function |
WO2000046385A1 (en) * | 1999-02-03 | 2000-08-10 | The Children's Medical Center Corporation | Gene repair involving in vivo excision of targeting dna |
EP1147209A2 (en) | 1999-02-03 | 2001-10-24 | The Children's Medical Center Corporation | Gene repair involving the induction of double-stranded dna cleavage at a chromosomal target site |
US6794136B1 (en) | 2000-11-20 | 2004-09-21 | Sangamo Biosciences, Inc. | Iterative optimization in the design of binding proteins |
US7030215B2 (en) * | 1999-03-24 | 2006-04-18 | Sangamo Biosciences, Inc. | Position dependent recognition of GNN nucleotide triplets by zinc fingers |
US20030104526A1 (en) * | 1999-03-24 | 2003-06-05 | Qiang Liu | Position dependent recognition of GNN nucleotide triplets by zinc fingers |
WO2000064485A2 (en) | 1999-04-28 | 2000-11-02 | Genencor International, Inc. | Specifically targeted catalytic antagonists and uses thereof |
US7668658B2 (en) | 1999-10-13 | 2010-02-23 | Sequenom, Inc. | Methods for generating databases and databases for identifying polymorphic genetic markers |
US20030207297A1 (en) * | 1999-10-13 | 2003-11-06 | Hubert Koster | Methods for generating databases and databases for identifying polymorphic genetic markers |
KR20020064298A (ko) | 1999-10-13 | 2002-08-07 | 시쿼넘, 인코포레이티드 | 다형성 유전 마커를 동정하기 위한 데이타베이스 및 이의제조 방법 |
ATE355368T1 (de) * | 2000-01-24 | 2006-03-15 | Gendaq Ltd | Nucleinsäure bindende polypeptide gekennzeichnet durch flexible linker verbundene nucleinsäuredomäne |
CA2398590C (en) | 2000-02-08 | 2012-08-28 | Sangamo Biosciences, Inc. | Cells for drug discovery |
AU5391401A (en) * | 2000-04-28 | 2001-11-12 | Sangamo Biosciences Inc | Targeted modification of chromatin structure |
US6958214B2 (en) | 2000-07-10 | 2005-10-25 | Sequenom, Inc. | Polymorphic kinase anchor proteins and nucleic acids encoding the same |
US20030082561A1 (en) * | 2000-07-21 | 2003-05-01 | Takashi Sera | Zinc finger domain recognition code and uses thereof |
EP1303608A2 (en) * | 2000-07-21 | 2003-04-23 | Syngenta Participations AG | Zinc finger domain recognition code and uses thereof |
AU2884102A (en) * | 2000-12-07 | 2002-06-18 | Sangamo Biosciences Inc | Regulation of angiogenesis with zinc finger proteins |
US7067317B2 (en) * | 2000-12-07 | 2006-06-27 | Sangamo Biosciences, Inc. | Regulation of angiogenesis with zinc finger proteins |
ES2409080T3 (es) * | 2001-01-22 | 2013-06-24 | Sangamo Biosciences Inc. | Proteínas de unión con dedos de zinc modificadas |
WO2003027247A2 (en) | 2001-09-24 | 2003-04-03 | Sangamo Biosciences, Inc. | Modulation of stem cells using zinc finger proteins |
US7262054B2 (en) * | 2002-01-22 | 2007-08-28 | Sangamo Biosciences, Inc. | Zinc finger proteins for DNA binding and gene regulation in plants |
WO2003087341A2 (en) | 2002-01-23 | 2003-10-23 | The University Of Utah Research Foundation | Targeted chromosomal mutagenesis using zinc finger nucleases |
WO2003078619A1 (en) | 2002-03-15 | 2003-09-25 | Cellectis | Hybrid and single chain meganucleases and use thereof |
WO2009095742A1 (en) * | 2008-01-31 | 2009-08-06 | Cellectis | New i-crei derived single-chain meganuclease and uses thereof |
US20100151556A1 (en) * | 2002-03-15 | 2010-06-17 | Cellectis | Hybrid and single chain meganucleases and use thereof |
US20030232410A1 (en) * | 2002-03-21 | 2003-12-18 | Monika Liljedahl | Methods and compositions for using zinc finger endonucleases to enhance homologous recombination |
US7101697B2 (en) * | 2002-04-30 | 2006-09-05 | Charité—Universitätsmedizin Berlin | Restriction endonucleases, method of synthesis and use thereof |
CA2484676A1 (en) | 2002-05-03 | 2003-11-13 | Sequenom, Inc. | Kinase anchor protein muteins, peptides thereof, and related methods |
CA2396345C (en) * | 2002-07-31 | 2007-04-10 | Rousseau Metal Inc. | Frontal latch handle assembly |
US7361635B2 (en) * | 2002-08-29 | 2008-04-22 | Sangamo Biosciences, Inc. | Simultaneous modulation of multiple genes |
EP2806025B1 (en) | 2002-09-05 | 2019-04-03 | California Institute of Technology | Use of zinc finger nucleases to stimulate gene targeting |
US7563600B2 (en) | 2002-09-12 | 2009-07-21 | Combimatrix Corporation | Microarray synthesis and assembly of gene-length polynucleotides |
US7820378B2 (en) * | 2002-11-27 | 2010-10-26 | Sequenom, Inc. | Fragmentation-based methods and systems for sequence variation detection and discovery |
JP2006518372A (ja) | 2003-01-28 | 2006-08-10 | セレクティス | 脊椎動物の体組織においてエクスビボかつイントトで相同的組換えを誘発するためのメガヌクレアーゼの使用およびその応用 |
CA2523490A1 (en) * | 2003-04-25 | 2004-11-11 | Sequenom, Inc. | Fragmentation-based methods and systems for de novo sequencing |
US7888121B2 (en) | 2003-08-08 | 2011-02-15 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
US8409861B2 (en) * | 2003-08-08 | 2013-04-02 | Sangamo Biosciences, Inc. | Targeted deletion of cellular DNA sequences |
US11311574B2 (en) | 2003-08-08 | 2022-04-26 | Sangamo Therapeutics, Inc. | Methods and compositions for targeted cleavage and recombination |
US20120196370A1 (en) | 2010-12-03 | 2012-08-02 | Fyodor Urnov | Methods and compositions for targeted genomic deletion |
WO2005014791A2 (en) | 2003-08-08 | 2005-02-17 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
US9394565B2 (en) * | 2003-09-05 | 2016-07-19 | Agena Bioscience, Inc. | Allele-specific sequence variation analysis |
EP1678315B1 (en) | 2003-09-19 | 2011-08-03 | Sangamo BioSciences, Inc. | Engineered zinc finger proteins for regulation of gene expression |
WO2005049842A2 (en) | 2003-11-18 | 2005-06-02 | Bayer Bioscience N.V. | Improved targeted dna insertion in plants |
US7972854B2 (en) | 2004-02-05 | 2011-07-05 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
EP1727911B1 (en) * | 2004-03-26 | 2013-01-23 | Sequenom, Inc. | Base specific cleavage of methylation-specific amplification products in combination with mass analysis |
US7608394B2 (en) | 2004-03-26 | 2009-10-27 | Sequenom, Inc. | Methods and compositions for phenotype identification based on nucleic acid methylation |
US20060073501A1 (en) * | 2004-09-10 | 2006-04-06 | Van Den Boom Dirk J | Methods for long-range sequence analysis of nucleic acids |
CA2579677A1 (en) * | 2004-09-16 | 2006-03-30 | Sangamo Biosciences, Inc. | Compositions and methods for protein production |
CA2584984A1 (en) * | 2004-10-18 | 2006-04-27 | Codon Devices, Inc. | Methods for assembly of high fidelity synthetic polynucleotides |
US20070122817A1 (en) * | 2005-02-28 | 2007-05-31 | George Church | Methods for assembly of high fidelity synthetic polynucleotides |
CN101155921B (zh) | 2005-04-04 | 2013-02-06 | 拜尔作物科学公司 | 用于去除所选择dna序列的方法和手段 |
CN101273141B (zh) | 2005-07-26 | 2013-03-27 | 桑格摩生物科学股份有限公司 | 外源核酸序列的靶向整合和表达 |
EP2484758B1 (en) | 2005-10-18 | 2013-10-02 | Precision Biosciences | Rationally-designed meganucleases with altered sequence specificity and DNA-binding affinity |
WO2007060495A1 (en) * | 2005-10-25 | 2007-05-31 | Cellectis | I-crei homing endonuclease variants having novel cleavage specificity and use thereof |
US7627401B2 (en) | 2006-02-07 | 2009-12-01 | Glenbrook Associates, Inc. | System and method for remotely regulating the power consumption of an electric appliance |
CA2650414A1 (en) * | 2006-05-19 | 2007-11-29 | Sangamo Biosciences, Inc. | Methods and compositions for inactivation of dihydrofolate reductase |
EP2019839B1 (en) | 2006-05-25 | 2011-12-07 | Sangamo BioSciences, Inc. | Methods and compositions for gene inactivation |
AU2007284748B2 (en) | 2006-08-11 | 2013-05-16 | Corteva Agriscience Llc | Zinc finger nuclease-mediated homologous recombination |
WO2008027558A2 (en) | 2006-08-31 | 2008-03-06 | Codon Devices, Inc. | Iterative nucleic acid assembly using activation of vector-encoded traits |
WO2008037436A1 (en) | 2006-09-28 | 2008-04-03 | Bayer Bioscience N.V. | Methods and means for removal of a selected dna sequence |
US9217026B2 (en) * | 2006-11-13 | 2015-12-22 | Sangamo Biosciences, Inc. | Method of inactivating a glucocorticoid receptor gene in an isolated cell |
MX2009006303A (es) | 2006-12-14 | 2009-10-21 | Dow Agrosciences Llc | Proteinas de dedo de zinc no canonicas optimizadas. |
US8748146B2 (en) * | 2007-04-19 | 2014-06-10 | Celexion, Llc | Engineered nucleases and their uses for nucleic acid assembly |
DE602008003684D1 (de) | 2007-04-26 | 2011-01-05 | Sangamo Biosciences Inc | Gezielte integration in die ppp1r12c-position |
BRPI0812233B1 (pt) | 2007-06-05 | 2022-10-04 | Bayer Cropscience Ag | Processos para troca de uma sequência de dna-alvo no genoma de uma célula de planta ou planta por uma sequência de dna de interesse, e vetor de dna |
JP5770471B2 (ja) * | 2007-07-12 | 2015-08-26 | サンガモ バイオサイエンシーズ, インコーポレイテッド | α−1,6−フコシルトランスフェラーゼ(FUT8)遺伝子発現を不活性化するための方法および組成物 |
US8563314B2 (en) | 2007-09-27 | 2013-10-22 | Sangamo Biosciences, Inc. | Methods and compositions for modulating PD1 |
CN101883863B (zh) * | 2007-09-27 | 2018-01-23 | 桑格摩生物科学股份有限公司 | 生物活性核酸酶的快速体内鉴定 |
US8399218B2 (en) | 2007-09-27 | 2013-03-19 | Dow Agrosciences, Llc | Engineered zinc finger proteins targeting 5-enolpyruvyl shikimate-3-phosphate synthase genes |
US11235026B2 (en) | 2007-09-27 | 2022-02-01 | Sangamo Therapeutics, Inc. | Methods and compositions for modulating PD1 |
CA2703045C (en) | 2007-10-25 | 2017-02-14 | Sangamo Biosciences, Inc. | Methods and compositions for targeted integration |
CA2703079A1 (en) * | 2007-12-07 | 2009-06-18 | Precision Biosciences, Inc. | Rationally-designed meganucleases with recognition sequences found in dnase hypersensitive regions of the human genome |
WO2009114321A2 (en) * | 2008-03-11 | 2009-09-17 | Precision Biosciencs, Inc. | Rationally-designed meganucleases for maize genome engineering |
US20100071083A1 (en) * | 2008-03-12 | 2010-03-18 | Smith James J | Temperature-dependent meganuclease activity |
JP2011518555A (ja) | 2008-04-14 | 2011-06-30 | サンガモ バイオサイエンシーズ, インコーポレイテッド | 標的組込みのための線形ドナーコンストラクト |
WO2009146179A1 (en) | 2008-04-15 | 2009-12-03 | University Of Iowa Research Foundation | Zinc finger nuclease for the cftr gene and methods of use thereof |
AU2009241351A1 (en) * | 2008-04-28 | 2009-11-05 | Precision Biosciences, Inc. | Fusion molecules of rationally-designed DNA-binding proteins and effector domains |
AU2009258117B2 (en) * | 2008-06-10 | 2014-10-09 | Sangamo Therapeutics, Inc. | Methods and compositions for generation of Bax- and Bak-deficient cell lines |
JP2011527906A (ja) | 2008-07-14 | 2011-11-10 | プレシジョン バイオサイエンシズ,インク. | I−CreI由来メガヌクレアーゼの認識配列およびその使用 |
CN102123778B (zh) * | 2008-08-14 | 2014-10-29 | 李伟德 | 利用离心力的动态过滤装置 |
KR101759586B1 (ko) | 2008-08-22 | 2017-07-19 | 상가모 테라퓨틱스, 인코포레이티드 | 표적화된 단일가닥 분할 및 표적화된 통합을 위한 방법 및 조성물 |
US8153399B2 (en) | 2008-10-29 | 2012-04-10 | Sangamo Biosciences, Inc. | Methods and compositions for inactivating glutamine synthetase gene expression |
US20110023153A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Genomic editing of genes involved in alzheimer's disease |
US20110023144A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Genomic editing of genes involved in amyotrophyic lateral sclerosis disease |
US20110023143A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Genomic editing of neurodevelopmental genes in animals |
US20110023158A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Bovine genome editing with zinc finger nucleases |
US20110023159A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Ovine genome editing with zinc finger nucleases |
US20110023139A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Genomic editing of genes involved in cardiovascular disease |
US20110023150A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Genome editing of genes associated with schizophrenia in animals |
CA2745031C (en) | 2008-12-04 | 2018-08-14 | Sangamo Biosciences, Inc. | Genome editing in rats using zinc-finger nucleases |
US20110016540A1 (en) * | 2008-12-04 | 2011-01-20 | Sigma-Aldrich Co. | Genome editing of genes associated with trinucleotide repeat expansion disorders in animals |
US20110016546A1 (en) * | 2008-12-04 | 2011-01-20 | Sigma-Aldrich Co. | Porcine genome editing with zinc finger nucleases |
US20110016541A1 (en) * | 2008-12-04 | 2011-01-20 | Sigma-Aldrich Co. | Genome editing of sensory-related genes in animals |
US20110023149A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Genomic editing of genes involved in tumor suppression in animals |
US20110023152A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Genome editing of cognition related genes in animals |
US20110023157A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Equine genome editing with zinc finger nucleases |
US20110023156A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Feline genome editing with zinc finger nucleases |
US20110016542A1 (en) * | 2008-12-04 | 2011-01-20 | Sigma-Aldrich Co. | Canine genome editing with zinc finger nucleases |
US20110023147A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Genomic editing of prion disorder-related genes in animals |
US20110023146A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Genomic editing of genes involved in secretase-associated disorders |
US20110016543A1 (en) * | 2008-12-04 | 2011-01-20 | Sigma-Aldrich Co. | Genomic editing of genes involved in inflammation |
US20110023145A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Genomic editing of genes involved in autism spectrum disorders |
US20110023140A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Rabbit genome editing with zinc finger nucleases |
US20110023151A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Genome editing of abc transporters |
US20110016539A1 (en) * | 2008-12-04 | 2011-01-20 | Sigma-Aldrich Co. | Genome editing of neurotransmission-related genes in animals |
US20110023148A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Genome editing of addiction-related genes in animals |
US20110023141A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Genomic editing of genes involved with parkinson's disease |
US20110030072A1 (en) * | 2008-12-04 | 2011-02-03 | Sigma-Aldrich Co. | Genome editing of immunodeficiency genes in animals |
US20110023154A1 (en) * | 2008-12-04 | 2011-01-27 | Sigma-Aldrich Co. | Silkworm genome editing with zinc finger nucleases |
BRPI0922641B1 (pt) * | 2008-12-17 | 2021-10-26 | Dow Agrosciences Llc | Método de integração de uma ou mais sequências de ácido nucleico exógenas no genoma de uma célula vegetal |
US20110239315A1 (en) | 2009-01-12 | 2011-09-29 | Ulla Bonas | Modular dna-binding domains and methods of use |
EP2206723A1 (en) | 2009-01-12 | 2010-07-14 | Bonas, Ulla | Modular DNA-binding domains |
WO2010117464A1 (en) | 2009-04-09 | 2010-10-14 | Sangamo Biosciences, Inc. | Targeted integration into stem cells |
US8772008B2 (en) | 2009-05-18 | 2014-07-08 | Sangamo Biosciences, Inc. | Methods and compositions for increasing nuclease activity |
CA2765488C (en) | 2009-06-30 | 2018-01-02 | Sangamo Biosciences, Inc. | Rapid screening of biologically active nucleases and isolation of nuclease-modified cells |
CA2766123A1 (en) | 2009-07-08 | 2011-01-13 | Cellular Dynamics International, Inc. | Modified ips cells having a mutant form of human immunodeficiency virus (hiv) cellular entry gene |
EP2461819A4 (en) | 2009-07-28 | 2013-07-31 | Sangamo Biosciences Inc | METHODS AND COMPOSITIONS FOR TREATING TRI-NUCLEOTIDE REPEAT DISORDERS |
CA2770312A1 (en) * | 2009-08-11 | 2011-02-17 | Sangamo Biosciences, Inc. | Organisms homozygous for targeted modification |
EP2491127B1 (en) | 2009-10-22 | 2017-09-20 | Dow AgroSciences LLC | Engineered zinc finger proteins targeting plant genes involved in fatty acid biosynthesis |
WO2011056872A2 (en) | 2009-11-03 | 2011-05-12 | Gen9, Inc. | Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly |
US8956828B2 (en) | 2009-11-10 | 2015-02-17 | Sangamo Biosciences, Inc. | Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases |
WO2011066185A1 (en) | 2009-11-25 | 2011-06-03 | Gen9, Inc. | Microfluidic devices and methods for gene synthesis |
AU2010327998B2 (en) | 2009-12-10 | 2015-11-12 | Iowa State University Research Foundation, Inc. | TAL effector-mediated DNA modification |
WO2011085075A2 (en) | 2010-01-07 | 2011-07-14 | Gen9, Inc. | Assembly of high fidelity polynucleotides |
CA2787494C (en) * | 2010-01-22 | 2019-09-17 | Dow Agrosciences Llc | Targeted genomic alteration |
WO2011100058A1 (en) | 2010-02-09 | 2011-08-18 | Sangamo Biosciences, Inc. | Targeted genomic modification with partially single-stranded donor molecules |
CN102869779A (zh) | 2010-03-29 | 2013-01-09 | 宾夕法尼亚大学托管会 | 药理学诱导的转基因消融系统 |
US9315825B2 (en) | 2010-03-29 | 2016-04-19 | The Trustees Of The University Of Pennsylvania | Pharmacologically induced transgene ablation system |
BR112012027532A2 (pt) | 2010-04-26 | 2020-10-13 | Sangamo Biosciences, Inc. | edição de genoma de um locus rosa usando nucleases de dedo de zinco. |
PL2566972T3 (pl) | 2010-05-03 | 2020-06-29 | Sangamo Therapeutics, Inc. | Kompozycje do wiązania modułów z motywem palca cynkowego |
WO2011146121A1 (en) | 2010-05-17 | 2011-11-24 | Sangamo Biosciences, Inc. | Novel dna-binding proteins and uses thereof |
CN109504700A (zh) | 2010-06-09 | 2019-03-22 | 拜尔作物科学公司 | 植物基因组改造中常用的在核苷酸序列上修饰植物基因组的方法和工具 |
US9593317B2 (en) | 2010-06-09 | 2017-03-14 | Bayer Cropscience Nv | Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering |
CA2993567C (en) | 2010-07-21 | 2022-06-28 | Sangamo Biosciences, Inc. | Methods and compositions for modification of a t-cell receptor gene |
CN103168101A (zh) | 2010-07-23 | 2013-06-19 | 西格马-奥尔德里奇有限责任公司 | 使用靶向核酸内切酶和单链核酸的基因组编辑 |
US9566352B2 (en) | 2010-09-27 | 2017-02-14 | Sangamo Biosciences, Inc. | Methods and compositions for inhibiting viral entry into cells |
ES2562421T3 (es) | 2010-10-12 | 2016-03-04 | The Children's Hospital Of Philadelphia | Métodos y composiciones para tratar la hemofilia B |
CN103502448B (zh) | 2010-11-12 | 2017-03-29 | Gen9股份有限公司 | 核酸合成的方法和设备 |
WO2012064975A1 (en) | 2010-11-12 | 2012-05-18 | Gen9, Inc. | Protein arrays and methods of using and making the same |
CA2821547A1 (en) | 2010-12-29 | 2012-07-05 | Sigma-Aldrich Co. Llc | Cells having disrupted expression of proteins involved in adme and toxicology processes |
WO2012094132A1 (en) | 2011-01-05 | 2012-07-12 | Sangamo Biosciences, Inc. | Methods and compositions for gene correction |
MX348785B (es) | 2011-06-06 | 2017-06-28 | Bayer Cropscience Nv | Metodos y medios para modificar un genoma vegetal en un sitio preseleccionado. |
CN103781900A (zh) | 2011-06-30 | 2014-05-07 | 西格马-奥尔德里奇有限责任公司 | 缺乏CMP-N-乙酰神经氨酸羟化酶和/或糖蛋白α-1,3-半乳糖基转移酶的细胞 |
EP2737063B1 (en) | 2011-07-25 | 2016-06-01 | Sangamo BioSciences, Inc. | Methods and compositions for alteration of a cystic fibrosis transmembrane conductance regulator (cftr) gene |
MX346439B (es) | 2011-08-22 | 2017-03-17 | Bayer Cropscience Nv | Métodos y medios para modificar un genoma vegetal. |
WO2013032850A2 (en) | 2011-08-26 | 2013-03-07 | Gen9, Inc. | Compositions and methods for high fidelity assembly of nucleic acids |
AU2012312260B2 (en) | 2011-09-21 | 2017-08-31 | Sangamo Therapeutics, Inc. | Methods and compositions for regulation of transgene expression |
US20140251317A1 (en) | 2011-10-12 | 2014-09-11 | Bayer Cropscience Ag | Plants with decreased activity of a starch dephosphorylating enzyme |
AU2013201323A1 (en) | 2011-10-12 | 2013-05-02 | Bayer Cropscience Ag | Plants with decreased activity of a starch dephosphorylating enzyme |
CA3099582A1 (en) | 2011-10-27 | 2013-05-02 | Sangamo Biosciences, Inc. | Methods and compositions for modification of the hprt locus |
JP6144691B2 (ja) | 2011-11-16 | 2017-06-07 | サンガモ セラピューティクス, インコーポレイテッド | 修飾されたdna結合タンパク質およびその使用 |
BR112014016614B1 (pt) | 2012-01-11 | 2022-02-01 | Sigma-Aldrich Co. Llc | Métodos para produzir uma célula deficiente em manosil (alfa-1,3-)-glicoproteína beta-1,2- nacetilglucosa-miniltransferase i (mgat1) e produzir uma proteína recombinante apresentando um ou mais resíduos de manose terminal |
BR112014021104B1 (pt) | 2012-02-29 | 2023-03-28 | Sangamo Biosciences, Inc | Proteína de fusão de ocorrência não natural compreendendo um domínio de ligação de dna de dedo de zinco manipulado que se liga a um gene htt, seu uso, método in vitro de modificação da expressão de um gene htt em uma célula, e método de geração de um sistema modelo para o estudo da doença de huntington |
US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
CN104245940A (zh) | 2012-04-23 | 2014-12-24 | 拜尔作物科学公司 | 植物中的靶向基因组工程 |
EP4001427A1 (en) | 2012-04-24 | 2022-05-25 | Gen9, Inc. | Methods for sorting nucleic acids and multiplexed preparative in vitro cloning |
NZ701060A (en) | 2012-05-02 | 2016-10-28 | Sangamo Biosciences Inc | Targeted modification of malate dehydrogenase |
RU2650819C2 (ru) | 2012-05-07 | 2018-04-17 | Сангамо Терапьютикс, Инк. | Способы и композиции для опосредованной нуклеазой направленной интеграции трансгенов |
WO2013169398A2 (en) | 2012-05-09 | 2013-11-14 | Georgia Tech Research Corporation | Systems and methods for improving nuclease specificity and activity |
RU2014153918A (ru) | 2012-06-12 | 2016-07-27 | Дженентек, Инк. | Способы и композиции для получения условно нокаутных аллелей |
CA2877290A1 (en) | 2012-06-19 | 2013-12-27 | Daniel F. Voytas | Gene targeting in plants using dna viruses |
CN113512577A (zh) | 2012-06-25 | 2021-10-19 | Gen9股份有限公司 | 用于核酸组装和高通量测序的方法 |
JP6329537B2 (ja) | 2012-07-11 | 2018-05-23 | サンガモ セラピューティクス, インコーポレイテッド | 生物学的薬剤の送達のための方法および組成物 |
WO2014011237A1 (en) | 2012-07-11 | 2014-01-16 | Sangamo Biosciences, Inc. | Methods and compositions for the treatment of lysosomal storage diseases |
US10648001B2 (en) | 2012-07-11 | 2020-05-12 | Sangamo Therapeutics, Inc. | Method of treating mucopolysaccharidosis type I or II |
KR102218562B1 (ko) | 2012-08-29 | 2021-02-19 | 상가모 테라퓨틱스, 인코포레이티드 | 유전적 병태를 치료하기 위한 방법 및 조성물 |
US9937205B2 (en) | 2012-09-04 | 2018-04-10 | The Trustees Of The University Of Pennsylvania | Inhibition of diacylglycerol kinase to augment adoptive T cell transfer |
UA118090C2 (uk) | 2012-09-07 | 2018-11-26 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив |
CN105264067B (zh) | 2012-09-07 | 2020-11-10 | 美国陶氏益农公司 | Fad3性能基因座及相应的能够诱导靶向断裂的靶位点特异性结合蛋白 |
AU2013329186B2 (en) | 2012-10-10 | 2019-02-14 | Sangamo Therapeutics, Inc. | T cell modifying compounds and uses thereof |
NZ707560A (en) | 2012-11-01 | 2019-10-25 | Cellectis | Plants for production of therapeutic proteins |
MX2015007574A (es) | 2012-12-13 | 2015-10-22 | Dow Agrosciences Llc | Gen de precision dirigido a un locus particular en el maiz. |
CA2895909C (en) | 2012-12-21 | 2021-07-06 | Cellectis | Potatoes with reduced cold-induced sweetening |
EP3919505B1 (en) | 2013-01-16 | 2023-08-30 | Emory University | Uses of cas9-nucleic acid complexes |
WO2014204578A1 (en) | 2013-06-21 | 2014-12-24 | The General Hospital Corporation | Using rna-guided foki nucleases (rfns) to increase specificity for rna-guided genome editing |
KR102210319B1 (ko) | 2013-03-15 | 2021-02-01 | 더 제너럴 하스피탈 코포레이션 | 특정 게놈 좌위에 대한 유전적 및 후성적 조절 단백질의 rna-안내 표적화 |
US10113162B2 (en) | 2013-03-15 | 2018-10-30 | Cellectis | Modifying soybean oil composition through targeted knockout of the FAD2-1A/1B genes |
US10760064B2 (en) | 2013-03-15 | 2020-09-01 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
CA2906970C (en) | 2013-03-21 | 2021-05-18 | Ospedale San Raffaele Srl | Targeted disruption of t cell receptor genes using engineered zinc finger protein nucleases |
WO2014161821A1 (en) | 2013-04-02 | 2014-10-09 | Bayer Cropscience Nv | Targeted genome engineering in eukaryotes |
CN105263312A (zh) | 2013-04-05 | 2016-01-20 | 美国陶氏益农公司 | 用于在植物基因组内整合外源序列的方法和组合物 |
WO2014186435A2 (en) | 2013-05-14 | 2014-11-20 | University Of Georgia Research Foundation, Inc. | Compositions and methods for reducing neointima formation |
CN105683376A (zh) | 2013-05-15 | 2016-06-15 | 桑格摩生物科学股份有限公司 | 用于治疗遗传病状的方法和组合物 |
JP6588438B2 (ja) | 2013-08-28 | 2019-10-09 | サンガモ セラピューティクス, インコーポレイテッド | Dna結合ドメインと切断ドメインとを連結するための組成物 |
KR20240042200A (ko) | 2013-09-11 | 2024-04-01 | 이글 바이오로직스 인코퍼레이티드 | 점도저하제를 함유하는 액체 단백질 제형 |
CN110713995B (zh) | 2013-10-17 | 2023-08-01 | 桑格摩生物科学股份有限公司 | 用于核酸酶介导的基因组工程改造的递送方法和组合物 |
MX360318B (es) | 2013-11-04 | 2018-10-29 | Dow Agrosciences Llc | Óptimos loci de maíz. |
BR102014027438B1 (pt) | 2013-11-04 | 2022-09-27 | Dow Agrosciences Llc | Molécula de ácido nucleico recombinante e método de produção de uma célula vegetal transgênica |
US9909131B2 (en) | 2013-11-04 | 2018-03-06 | Dow Agrosciences Llc | Optimal soybean loci |
NZ719477A (en) | 2013-11-11 | 2022-05-27 | Sangamo Therapeutics Inc | Methods and compositions for treating huntington’s disease |
SI3068881T1 (sl) | 2013-11-13 | 2019-05-31 | Children's Medical Center Corporation | Z nukleazo posredovano uravnavanje izražanja genov |
EP2878667A1 (en) | 2013-11-29 | 2015-06-03 | Institut Pasteur | TAL effector means useful for partial or full deletion of DNA tandem repeats |
ES2813367T3 (es) | 2013-12-09 | 2021-03-23 | Sangamo Therapeutics Inc | Métodos y composiciones para ingeniería genómica |
PL3102673T3 (pl) | 2014-02-03 | 2020-11-02 | Sangamo Therapeutics, Inc. | Sposoby i kompozycje do leczenia talasemii beta |
MX2016010100A (es) | 2014-02-05 | 2016-10-07 | Fraunhofer Ges Forschung | Secuenciacion libre de error de acido desoxirribonucleico (adn). |
SG11201607038TA (en) | 2014-03-04 | 2016-09-29 | Sigma Aldrich Co Llc | Viral resistant cells and uses thereof |
AU2015231353B2 (en) | 2014-03-18 | 2020-11-05 | Sangamo Therapeutics, Inc. | Methods and compositions for regulation of zinc finger protein expression |
WO2015164378A1 (en) | 2014-04-22 | 2015-10-29 | Q-State Biosciences, Inc. | Analysis of compounds for pain and sensory disorders |
US9522936B2 (en) | 2014-04-24 | 2016-12-20 | Sangamo Biosciences, Inc. | Engineered transcription activator like effector (TALE) proteins |
US11918695B2 (en) | 2014-05-09 | 2024-03-05 | Yale University | Topical formulation of hyperbranched polymer-coated particles |
WO2015172153A1 (en) | 2014-05-09 | 2015-11-12 | Yale University | Topical formulation of hyperbranched polyglycerol-coated particles thereof |
EP3142707A4 (en) | 2014-05-13 | 2018-02-21 | Sangamo Therapeutics, Inc. | Methods and compositions for prevention or treatment of a disease |
US10066241B2 (en) | 2014-05-30 | 2018-09-04 | The Board Of Trustees Of The Leland Stanford Junior University | Compositions and methods of delivering treatments for latent viral infections |
WO2015188056A1 (en) | 2014-06-05 | 2015-12-10 | Sangamo Biosciences, Inc. | Methods and compositions for nuclease design |
WO2015193858A1 (en) | 2014-06-20 | 2015-12-23 | Cellectis | Potatoes with reduced granule-bound starch synthase |
PT3169778T (pt) | 2014-07-14 | 2024-01-29 | Univ Washington State | Knock-out de nanos que conduz à ablação de células da linha germinativa |
CN107075483A (zh) | 2014-07-15 | 2017-08-18 | 朱诺治疗学股份有限公司 | 用于过继细胞治疗的工程改造的细胞 |
US11071289B2 (en) | 2014-08-14 | 2021-07-27 | Biocytogen Boston Corp | DNA knock-in system |
WO2016044416A1 (en) | 2014-09-16 | 2016-03-24 | Sangamo Biosciences, Inc. | Methods and compositions for nuclease-mediated genome engineering and correction in hematopoietic stem cells |
KR102497368B1 (ko) | 2014-10-01 | 2023-02-10 | 이글 바이올로직스 인코포레이티드 | 점도-저하제를 함유하는 폴리삭카라이드 및 핵산 제형 |
KR102630014B1 (ko) | 2014-10-01 | 2024-01-25 | 더 제너럴 하스피탈 코포레이션 | 뉴클레아제-유도 상동성-지정 복구의 효율 증가 방법 |
US10889834B2 (en) | 2014-12-15 | 2021-01-12 | Sangamo Therapeutics, Inc. | Methods and compositions for enhancing targeted transgene integration |
WO2016118726A2 (en) | 2015-01-21 | 2016-07-28 | Sangamo Biosciences, Inc. | Methods and compositions for identification of highly specific nucleases |
US10048275B2 (en) | 2015-03-13 | 2018-08-14 | Q-State Biosciences, Inc. | Cardiotoxicity screening methods |
ES2959608T3 (es) | 2015-04-03 | 2024-02-27 | Dana Farber Cancer Inst Inc | Composición y métodos de edición del genoma de células B |
US10738290B2 (en) | 2015-04-21 | 2020-08-11 | Novartis Ag | RNA-guided gene editing system and uses thereof |
DK3289080T3 (da) | 2015-04-30 | 2021-11-08 | Univ Columbia | Genterapi til autosomalt dominante sygdomme |
US10288863B2 (en) | 2015-05-21 | 2019-05-14 | Q-State Biosciences, Inc. | Optogenetics microscope |
EP3303586A1 (en) | 2015-05-29 | 2018-04-11 | Juno Therapeutics, Inc. | Composition and methods for regulating inhibitory interactions in genetically engineered cells |
US10117911B2 (en) | 2015-05-29 | 2018-11-06 | Agenovir Corporation | Compositions and methods to treat herpes simplex virus infections |
US20160346360A1 (en) | 2015-05-29 | 2016-12-01 | Agenovir Corporation | Compositions and methods for cell targeted hpv treatment |
US9957501B2 (en) | 2015-06-18 | 2018-05-01 | Sangamo Therapeutics, Inc. | Nuclease-mediated regulation of gene expression |
EP3322297A4 (en) | 2015-07-13 | 2019-04-17 | Sangamo Therapeutics, Inc. | RELEASE METHOD AND COMPOSITIONS FOR NUCLEASE-DERIVED GENOMINE ENGINEERING |
MA42895A (fr) | 2015-07-15 | 2018-05-23 | Juno Therapeutics Inc | Cellules modifiées pour thérapie cellulaire adoptive |
EP3331355B1 (en) | 2015-08-06 | 2024-04-03 | The Curators of the University of Missouri | Porcine reproductive and respiratory syndrome virus (prrsv)-resistant porcine and cells having modified cd163 genes |
US9926546B2 (en) | 2015-08-28 | 2018-03-27 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
US9512446B1 (en) | 2015-08-28 | 2016-12-06 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
US10837024B2 (en) | 2015-09-17 | 2020-11-17 | Cellectis | Modifying messenger RNA stability in plant transformations |
BR112018012235A2 (pt) | 2015-12-18 | 2018-12-04 | Sangamo Therapeutics Inc | rompimento alvejado do receptor de células de mhc |
AU2016369490C1 (en) | 2015-12-18 | 2021-12-23 | Sangamo Therapeutics, Inc. | Targeted disruption of the T cell receptor |
AU2017207818B2 (en) | 2016-01-15 | 2024-03-28 | Regents Of The University Of Minnesota | Methods and compositions for the treatment of neurologic disease |
EP3410843A1 (en) | 2016-02-02 | 2018-12-12 | Cellectis | Modifying soybean oil composition through targeted knockout of the fad3a/b/c genes |
EP3769775A3 (en) | 2016-02-02 | 2021-03-17 | Sangamo Therapeutics, Inc. | Compositions for linking dna-binding domains and cleavage domains |
US11136597B2 (en) | 2016-02-16 | 2021-10-05 | Yale University | Compositions for enhancing targeted gene editing and methods of use thereof |
CA3014795A1 (en) | 2016-02-16 | 2017-08-24 | Yale University | Compositions and methods for treatment of cystic fibrosis |
EP3423158B1 (en) | 2016-02-24 | 2023-11-15 | The Rockefeller University | Embryonic cell-based therapeutic candidate screening systems, models for huntington's disease and uses thereof |
CN116059245A (zh) | 2016-03-23 | 2023-05-05 | 加利福尼亚大学董事会 | 治疗线粒体障碍的方法 |
US20190117799A1 (en) | 2016-04-01 | 2019-04-25 | The Brigham And Women's Hospital, Inc. | Stimuli-responsive nanoparticles for biomedical applications |
US11514331B2 (en) | 2016-04-27 | 2022-11-29 | Massachusetts Institute Of Technology | Sequence-controlled polymer random access memory storage |
WO2017189870A1 (en) | 2016-04-27 | 2017-11-02 | Massachusetts Institute Of Technology | Stable nanoscale nucleic acid assemblies and methods thereof |
US11021713B2 (en) | 2016-05-25 | 2021-06-01 | Cargill, Incorporated | Engineered nucleases to generate deletion mutants in plants |
WO2017202715A1 (en) | 2016-05-26 | 2017-11-30 | Nunhems B.V. | Seedless fruit producing plants |
CA3025523A1 (en) | 2016-05-27 | 2017-11-30 | Aadigen, Llc | Peptides and nanoparticles for intracellular delivery of genome-editing molecules |
MA45491A (fr) | 2016-06-27 | 2019-05-01 | Juno Therapeutics Inc | Épitopes à restriction cmh-e, molécules de liaison et procédés et utilisations associés |
EP3475446A1 (en) | 2016-06-27 | 2019-05-01 | Juno Therapeutics, Inc. | Method of identifying peptide epitopes, molecules that bind such epitopes and related uses |
EP3484870B1 (en) | 2016-07-13 | 2022-11-16 | Vertex Pharmaceuticals Incorporated | Methods, compositions and kits for increasing genome editing efficiency |
JP2019520844A (ja) | 2016-07-21 | 2019-07-25 | マックスサイト インコーポレーティッド | ゲノムdnaを改変するための方法および組成物 |
KR20190038613A (ko) | 2016-08-11 | 2019-04-08 | 더 잭슨 래보라토리 | 유전자 변형된 면역결핍 비-인간 동물에서의 개선된 인간 적혈구 생존에 관한 방법 및 조성물 |
CN110325635B (zh) | 2016-08-24 | 2023-12-26 | 桑格摩生物治疗股份有限公司 | 使用工程化的核酸酶调控基因表达 |
KR102455249B1 (ko) | 2016-08-24 | 2022-10-17 | 상가모 테라퓨틱스, 인코포레이티드 | 가공된 표적 특이적 뉴클레아제 |
JP7179354B2 (ja) | 2016-08-29 | 2022-11-29 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 皮膚処置のためのイオン種に基づく局所処方物 |
US20190225974A1 (en) | 2016-09-23 | 2019-07-25 | BASF Agricultural Solutions Seed US LLC | Targeted genome optimization in plants |
CN110050061A (zh) | 2016-10-05 | 2019-07-23 | 富士胶片细胞动力公司 | 从具有MeCP2破坏的诱导多能干细胞生成成熟谱系 |
EP4190335A1 (en) | 2016-10-13 | 2023-06-07 | Juno Therapeutics, Inc. | Immunotherapy methods and compositions involving tryptophan metabolic pathway modulators |
MX2019004487A (es) | 2016-10-20 | 2020-02-07 | Sangamo Therapeutics Inc | Metodos y composiciones para el tratamiento de la enfermedad de fabry. |
WO2018081138A1 (en) | 2016-10-24 | 2018-05-03 | Yale University | Biodegradable contraceptive implants |
US11020492B2 (en) | 2016-10-31 | 2021-06-01 | Sangamo Therapeutics, Inc. | Gene correction of SCID-related genes in hematopoietic stem and progenitor cells |
CN109906030B (zh) | 2016-11-04 | 2022-03-18 | 安健基因公司 | 用于产生仅重链抗体的经基因修饰的非人动物和方法 |
UY37482A (es) | 2016-11-16 | 2018-05-31 | Cellectis | Métodos para alterar el contenido de aminoácidos en plantas mediante mutaciones de desplazamiento de marco |
US11332713B2 (en) | 2016-11-16 | 2022-05-17 | KSQ Therapeutics, Inc. | Gene-regulating compositions and methods for improved immunotherapy |
US11793833B2 (en) | 2016-12-02 | 2023-10-24 | Juno Therapeutics, Inc. | Engineered B cells and related compositions and methods |
ES2968892T3 (es) | 2016-12-08 | 2024-05-14 | Univ Case Western Reserve | Métodos y composiciones para aumentar la producción de mielina funcional |
WO2018112470A1 (en) | 2016-12-16 | 2018-06-21 | The Brigham And Women's Hospital, Inc. | Co-delivery of nucleic acids for simultaneous suppression and expression of target genes |
US20190390241A1 (en) | 2017-01-24 | 2019-12-26 | Sigma-Aldrich Co. Llc | Viral resistant cells and culture systems |
EP3599842A4 (en) | 2017-03-21 | 2020-12-30 | The Jackson Laboratory | GENETICALLY MODIFIED MOUSE EXPRESSING HUMAN APOE4 AND TREM.P.R47H AND ASSOCIATED PROCEDURES FOR USE |
WO2018187493A1 (en) | 2017-04-04 | 2018-10-11 | Yale University | Compositions and methods for in utero delivery |
BR112019021993A2 (pt) | 2017-04-20 | 2020-05-12 | Oregon Health & Science University | Correção de gene humano |
AU2018260469A1 (en) | 2017-04-25 | 2019-11-14 | Cellectis | Alfalfa with reduced lignin composition |
RU2019140867A (ru) | 2017-05-12 | 2021-06-15 | Зе Джексон Лаборатори | Nsg мыши, не имеющие mhc класса i и класса ii |
KR20200027512A (ko) | 2017-06-20 | 2020-03-12 | 엥스띠뛰 퀴리 | Suv39h1에 결함이 있는 면역 세포 |
WO2019089982A1 (en) | 2017-11-01 | 2019-05-09 | Juno Therapeutics, Inc. | Method of assessing activity of recombinant antigen receptors |
WO2019094928A1 (en) | 2017-11-10 | 2019-05-16 | Massachusetts Institute Of Technology | Microbial production of pure single stranded nucleic acids |
US10953036B2 (en) | 2017-11-20 | 2021-03-23 | University Of Georgia Research Foundation, Inc. | Compositions and methods of modulating HIF-2A to improve muscle generation and repair |
EP3501268B1 (en) | 2017-12-22 | 2021-09-15 | KWS SAAT SE & Co. KGaA | Regeneration of plants in the presence of histone deacetylase inhibitors |
EP3508581A1 (en) | 2018-01-03 | 2019-07-10 | Kws Saat Se | Regeneration of genetically modified plants |
CA3086620A1 (en) | 2018-01-12 | 2019-07-18 | Basf Se | Gene underlying the number of spikelets per spike qtl in wheat on chromosome 7a |
CN111757876B (zh) | 2018-01-17 | 2024-03-22 | 沃泰克斯药物股份有限公司 | Dna-pk抑制剂 |
EP3740479A1 (en) | 2018-01-17 | 2020-11-25 | Vertex Pharmaceuticals Incorporated | Dna-pk inhibitors |
EA202091707A1 (ru) | 2018-01-17 | 2020-12-02 | Вертекс Фармасьютикалз Инкорпорейтед | Хиноксалиноновые соединения, композиции, способы и наборы для повышения эффективности редактирования генома |
AU2019218118A1 (en) | 2018-02-08 | 2020-08-13 | Sangamo Therapeutics, Inc. | Engineered target specific nucleases |
BR112020018658A2 (pt) | 2018-03-15 | 2020-12-29 | KSQ Therapeutics, Inc. | Composições de regulação gênica e métodos para imu-noterapia aprimorada |
CA3093915A1 (en) | 2018-03-15 | 2019-09-19 | KSQ Therapeutics, Inc. | Gene-regulating compositions and methods for improved immunotherapy |
US20210017509A1 (en) | 2018-03-23 | 2021-01-21 | The Trustees Of Columbia University In The City Of New York | Gene Editing for Autosomal Dominant Diseases |
EP3545756A1 (en) | 2018-03-28 | 2019-10-02 | KWS SAAT SE & Co. KGaA | Regeneration of plants in the presence of inhibitors of the histone methyltransferase ezh2 |
RU2020135966A (ru) | 2018-04-05 | 2022-05-06 | Джуно Терапьютикс, Инк. | Способы получения клеток, экспрессирующих рекомбинантный рецептор, и родственные композиции |
MA52207A (fr) | 2018-04-05 | 2021-02-17 | Editas Medicine Inc | Lymphocytes t exprimant un récepteur recombinant, polynucléotides et procédés associés |
EP3567111A1 (en) | 2018-05-09 | 2019-11-13 | KWS SAAT SE & Co. KGaA | Gene for resistance to a pathogen of the genus heterodera |
US11291176B2 (en) | 2018-06-15 | 2022-04-05 | Nunhems B.V. | Seedless watermelon plants comprising modifications in an ABC transporter gene |
BR112020025349A2 (pt) | 2018-06-15 | 2021-03-09 | KWS SAAT SE & Co. KGaA | Métodos para melhorar a engenharia e regeneração do genoma em planta |
EP3807299A1 (en) | 2018-06-15 | 2021-04-21 | KWS SAAT SE & Co. KGaA | Methods for enhancing genome engineering efficiency |
CA3103500A1 (en) | 2018-06-15 | 2019-12-19 | KWS SAAT SE & Co. KGaA | Methods for improving genome engineering and regeneration in plant ii |
US20210195879A1 (en) | 2018-06-21 | 2021-07-01 | The Jackson Laboratory | Genetically modified mouse models of alzheimer's disease |
EP3830278A4 (en) | 2018-08-01 | 2022-05-25 | University of Georgia Research Foundation, Inc. | COMPOSITIONS AND METHODS FOR ENHANCING EMBRYO DEVELOPMENT |
KR20210049124A (ko) | 2018-08-23 | 2021-05-04 | 상가모 테라퓨틱스, 인코포레이티드 | 조작된 표적 특이적 염기 편집기 |
US20210338815A1 (en) | 2018-08-31 | 2021-11-04 | Yale University | Compositions and methods for enhancing triplex and nuclease-based gene editing |
WO2020051283A1 (en) | 2018-09-05 | 2020-03-12 | The Regents Of The University Of California | Generation of heritably gene-edited plants without tissue culture |
EP3623379A1 (en) | 2018-09-11 | 2020-03-18 | KWS SAAT SE & Co. KGaA | Beet necrotic yellow vein virus (bnyvv)-resistance modifying gene |
CN113015741A (zh) | 2018-09-18 | 2021-06-22 | 桑格摩生物治疗股份有限公司 | 程序性细胞死亡1(pd1)特异性核酸酶 |
JP2022506974A (ja) | 2018-11-08 | 2022-01-17 | トリトン アルジー イノベーションズ インコーポレイテッド | 藻類由来のヘムを食用の製品に組み込むための組成物及び方法 |
KR20200071198A (ko) | 2018-12-10 | 2020-06-19 | 네오이뮨텍, 인코퍼레이티드 | Nrf2 발현 조절 기반 T 세포 항암면역치료법 |
US20220064657A1 (en) | 2019-01-04 | 2022-03-03 | Cargill, Incorporated | Engineered nucleases to generate mutations in plants |
EP3914708A1 (en) | 2019-01-24 | 2021-12-01 | Massachusetts Institute Of Technology | Nucleic acid nanostructure platform for antigen presentation and vaccine formulations formed therefrom |
CN113490747A (zh) | 2019-01-29 | 2021-10-08 | 华威大学 | 用于提高基因组工程化效率的方法 |
EP3708651A1 (en) | 2019-03-12 | 2020-09-16 | KWS SAAT SE & Co. KGaA | Improving plant regeneration |
MA55531A (fr) | 2019-04-02 | 2022-02-09 | Sangamo Therapeutics Inc | Procédés pour le traitement de béta-thalassémie |
EP3953485A4 (en) | 2019-04-10 | 2023-05-17 | University of Utah Research Foundation | MODULATION OF HTRA1 FOR THE TREATMENT OF ARMD |
CN114025788A (zh) | 2019-05-01 | 2022-02-08 | 朱诺治疗学股份有限公司 | 从经修饰的tgfbr2基因座表达重组受体的细胞、相关多核苷酸和方法 |
WO2020223571A1 (en) | 2019-05-01 | 2020-11-05 | Juno Therapeutics, Inc. | Cells expressing a chimeric receptor from a modified cd247 locus, related polynucleotides and methods |
US11905532B2 (en) | 2019-06-25 | 2024-02-20 | Massachusetts Institute Of Technology | Compositions and methods for molecular memory storage and retrieval |
EP3757219A1 (en) | 2019-06-28 | 2020-12-30 | KWS SAAT SE & Co. KGaA | Enhanced plant regeneration and transformation by using grf1 booster gene |
MX2022000922A (es) | 2019-07-23 | 2022-05-03 | Mnemo Therapeutics | Celulas inmunes defectuosas para suv39h1. |
US20230227583A1 (en) | 2019-08-30 | 2023-07-20 | Yale University | Compositions and methods for delivery of nucleic acids to cells |
CA3157872A1 (en) | 2019-11-12 | 2021-05-20 | Otto Torjek | Gene for resistance to a pathogen of the genus heterodera |
US20230212613A1 (en) | 2020-05-06 | 2023-07-06 | Cellectis S.A. | Methods for targeted insertion of exogenous sequences in cellular genomes |
AU2021269103A1 (en) | 2020-05-06 | 2022-12-15 | Cellectis S.A. | Methods to genetically modify cells for delivery of therapeutic proteins |
CN115835873A (zh) | 2020-05-13 | 2023-03-21 | 朱诺治疗学股份有限公司 | 用于产生表达重组受体的供体分批细胞的方法 |
KR20230042283A (ko) | 2020-06-26 | 2023-03-28 | 주노 테라퓨틱스 게엠베하 | 재조합 수용체를 조건부로 발현하는 조작된 t 세포, 관련된 폴리뉴클레오티드 및 방법 |
AU2021316727A1 (en) | 2020-07-30 | 2023-03-02 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Immune cells defective for SOCS1 |
EP4204545A2 (en) | 2020-08-25 | 2023-07-05 | Kite Pharma, Inc. | T cells with improved functionality |
CN116802203A (zh) | 2020-11-04 | 2023-09-22 | 朱诺治疗学股份有限公司 | 从经修饰的恒定cd3免疫球蛋白超家族链基因座表达嵌合受体的细胞、相关多核苷酸和方法 |
WO2022101641A1 (en) | 2020-11-16 | 2022-05-19 | Pig Improvement Company Uk Limited | Influenza a-resistant animals having edited anp32 genes |
EP4019639A1 (en) | 2020-12-22 | 2022-06-29 | KWS SAAT SE & Co. KGaA | Promoting regeneration and transformation in beta vulgaris |
EP4019638A1 (en) | 2020-12-22 | 2022-06-29 | KWS SAAT SE & Co. KGaA | Promoting regeneration and transformation in beta vulgaris |
JP2024511420A (ja) | 2021-03-22 | 2024-03-13 | ジュノー セラピューティクス インコーポレイテッド | ウイルスベクター粒子の効力を評価する方法 |
EP4337769A1 (en) | 2021-05-10 | 2024-03-20 | SQZ Biotechnologies Company | Methods for delivering genome editing molecules to the nucleus or cytosol of a cell and uses thereof |
WO2022251644A1 (en) | 2021-05-28 | 2022-12-01 | Lyell Immunopharma, Inc. | Nr4a3-deficient immune cells and uses thereof |
KR20240027676A (ko) | 2021-06-02 | 2024-03-04 | 라이엘 이뮤노파마, 인크. | Nr4a3-결핍 면역 세포 및 이의 용도 |
WO2022271548A2 (en) | 2021-06-23 | 2022-12-29 | Massachusetts Institute Of Technology | Compositions, methods and systems for the delivery of gene editing material to cells |
WO2023064924A1 (en) | 2021-10-14 | 2023-04-20 | Codiak Biosciences, Inc. | Modified producer cells for extracellular vesicle production |
CA3237482A1 (en) | 2021-11-03 | 2023-05-11 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone | Precise genome editing using retrons |
CA3237696A1 (en) | 2021-11-08 | 2023-05-11 | Progentos Therapeutics, Inc. | Platelet-derived growth factor receptor (pdgfr) alpha inhibitors and uses thereof |
WO2023081900A1 (en) | 2021-11-08 | 2023-05-11 | Juno Therapeutics, Inc. | Engineered t cells expressing a recombinant t cell receptor (tcr) and related systems and methods |
TW202328201A (zh) | 2021-11-09 | 2023-07-16 | 美商安進公司 | 產生抗體肽軛合物的方法 |
WO2023105244A1 (en) | 2021-12-10 | 2023-06-15 | Pig Improvement Company Uk Limited | Editing tmprss2/4 for disease resistance in livestock |
WO2023126458A1 (en) | 2021-12-28 | 2023-07-06 | Mnemo Therapeutics | Immune cells with inactivated suv39h1 and modified tcr |
WO2023141602A2 (en) | 2022-01-21 | 2023-07-27 | Renagade Therapeutics Management Inc. | Engineered retrons and methods of use |
WO2023168397A1 (en) | 2022-03-04 | 2023-09-07 | Sigma-Aldrich Co. Llc | Metabolic selection via the asparagine biosynthesis pathway |
WO2023225665A1 (en) | 2022-05-19 | 2023-11-23 | Lyell Immunopharma, Inc. | Polynucleotides targeting nr4a3 and uses thereof |
EP4279085A1 (en) | 2022-05-20 | 2023-11-22 | Mnemo Therapeutics | Compositions and methods for treating a refractory or relapsed cancer or a chronic infectious disease |
WO2024013514A2 (en) | 2022-07-15 | 2024-01-18 | Pig Improvement Company Uk Limited | Gene edited livestock animals having coronavirus resistance |
WO2024044723A1 (en) | 2022-08-25 | 2024-02-29 | Renagade Therapeutics Management Inc. | Engineered retrons and methods of use |
WO2024062138A1 (en) | 2022-09-23 | 2024-03-28 | Mnemo Therapeutics | Immune cells comprising a modified suv39h1 gene |
WO2024064952A1 (en) | 2022-09-23 | 2024-03-28 | Lyell Immunopharma, Inc. | Methods for culturing nr4a-deficient cells overexpressing c-jun |
WO2024064958A1 (en) | 2022-09-23 | 2024-03-28 | Lyell Immunopharma, Inc. | Methods for culturing nr4a-deficient cells |
WO2024073686A1 (en) | 2022-09-30 | 2024-04-04 | Sigma-Aldrich Co. Llc | Metabolic selection via the serine biosynthesis pathway |
WO2024073692A1 (en) | 2022-09-30 | 2024-04-04 | Sigma-Aldrich Co. Llc | Metabolic selection via the glycine-formate biosynthesis pathway |
WO2024077174A1 (en) | 2022-10-05 | 2024-04-11 | Lyell Immunopharma, Inc. | Methods for culturing nr4a-deficient cells |
WO2024100604A1 (en) | 2022-11-09 | 2024-05-16 | Juno Therapeutics Gmbh | Methods for manufacturing engineered immune cells |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0589958A4 (en) * | 1991-06-11 | 1995-04-26 | Gen Hospital Corp | UNIVERSAL NUCLEASES ACTING ON A SPECIFIED SITE. |
US5436150A (en) * | 1992-04-03 | 1995-07-25 | The Johns Hopkins University | Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease |
CA2154581C (en) * | 1993-02-12 | 2008-12-16 | Srinivasan Chandrasegaran | Functional domains in flavobacterium okeanokoites (foki) restriction endonuclease |
-
1993
- 1993-09-27 US US08/126,564 patent/US5436150A/en not_active Expired - Lifetime
-
1994
- 1994-08-23 JP JP7510290A patent/JPH09505728A/ja not_active Withdrawn
- 1994-08-23 CA CA002170986A patent/CA2170986A1/en not_active Abandoned
- 1994-08-23 EP EP94925854A patent/EP0721502A4/en not_active Withdrawn
- 1994-08-23 WO PCT/US1994/009143 patent/WO1995009233A1/en not_active Application Discontinuation
- 1994-08-23 AU AU75636/94A patent/AU687435B2/en not_active Expired - Fee Related
-
2006
- 2006-05-23 JP JP2006143294A patent/JP4081119B2/ja not_active Expired - Lifetime
-
2007
- 2007-09-05 JP JP2007230093A patent/JP2008005851A/ja not_active Withdrawn
-
2008
- 2008-11-13 JP JP2008291366A patent/JP2009072201A/ja not_active Withdrawn
-
2012
- 2012-02-23 JP JP2012037730A patent/JP2012135316A/ja not_active Withdrawn
- 2012-12-21 JP JP2012279894A patent/JP2013081471A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
JP2009072201A (ja) | 2009-04-09 |
US5436150A (en) | 1995-07-25 |
JP2006254921A (ja) | 2006-09-28 |
JP2012135316A (ja) | 2012-07-19 |
JP4081119B2 (ja) | 2008-04-23 |
EP0721502A4 (en) | 1997-02-12 |
JP2013081471A (ja) | 2013-05-09 |
JP2008005851A (ja) | 2008-01-17 |
EP0721502A1 (en) | 1996-07-17 |
CA2170986A1 (en) | 1995-04-06 |
AU7563694A (en) | 1995-04-18 |
WO1995009233A1 (en) | 1995-04-06 |
AU687435B2 (en) | 1998-02-26 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JPH09505728A (ja) | フラボバクテリウム・オケアノコイテス(foki)制限エンドヌクレアーゼにおける機能ドメイン | |
EP0682699B1 (en) | FUNCTIONAL DOMAINS IN $i(FLAVOBACTERIUM OKEANOKOITES) (FOKI) RESTRICTION ENDONUCLEASE | |
US5792640A (en) | General method to clone hybrid restriction endonucleases using lig gene | |
US5356802A (en) | Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease | |
Rettberg et al. | A three-way junction and constituent stem-loops as the stimulator for programmed− 1 frameshifting in bacterial insertion sequence IS911 | |
US9353358B2 (en) | Sequence-specific engineered ribonuclease H and the method for determining the sequence preference of DNA-RNA hybrid binding proteins | |
WO1995032281A1 (en) | A METHOD FOR DIRECT CLONING OF NUCLEASE GENES IN $i(E. COLI) | |
EP0931835B1 (en) | Method for cloning and producing the PmeI restriction endonuclease | |
JPH06205683A (ja) | SphI制限エンドヌクレアーゼをコードする単離DNA及び該制限エンドヌクレアーゼを製造するための方法 | |
EP1179596A1 (en) | Nuclease | |
KR100757277B1 (ko) | 고호열성 리가아제 효소 및 이의 제조방법 | |
US5731185A (en) | Isolated DNA encoding the hphi restriction endonuclease and related methods for producing the same | |
US6764843B2 (en) | Method of cloning and expression of BsmBI restriction endonuclease and BsmBI methylase in E. coli and purification of BsmBI endonuclease | |
Li | Studies on FokI (A type IIS) restriction endonuclease | |
KR100449171B1 (ko) | Mj1 유전자를 포함하는 재조합 벡터 및 이를 이용한인테그라아제 mj1의 제조방법 | |
JP2007530046A (ja) | 新規モジュラーII型制限エンドヌクレアーゼCspCI、および新規特異性を有するエンドヌクレアーゼを製造するためのモジュラーエンドヌクレアーゼの用途 | |
JP2000157278A (ja) | 新規dna分解酵素 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20040302 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20040602 |
|
A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20040716 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20040901 |
|
A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20060124 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20060523 |
|
A911 | Transfer to examiner for re-examination before appeal (zenchi) |
Free format text: JAPANESE INTERMEDIATE CODE: A911 Effective date: 20060608 |
|
A912 | Re-examination (zenchi) completed and case transferred to appeal board |
Free format text: JAPANESE INTERMEDIATE CODE: A912 Effective date: 20061005 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20090107 |
|
A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20090119 |
|
A761 | Written withdrawal of application |
Free format text: JAPANESE INTERMEDIATE CODE: A761 Effective date: 20091009 |