JP2020526219A5 - - Google Patents

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Publication number
JP2020526219A5
JP2020526219A5 JP2020501517A JP2020501517A JP2020526219A5 JP 2020526219 A5 JP2020526219 A5 JP 2020526219A5 JP 2020501517 A JP2020501517 A JP 2020501517A JP 2020501517 A JP2020501517 A JP 2020501517A JP 2020526219 A5 JP2020526219 A5 JP 2020526219A5
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Japan
Prior art keywords
cell culture
substrate
container
flange
culture vessel
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JP2020501517A
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JP7195302B2 (ja
JP2020526219A (ja
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Priority claimed from PCT/US2018/042115 external-priority patent/WO2019014610A1/en
Publication of JP2020526219A publication Critical patent/JP2020526219A/ja
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Claims (7)

  1. 細胞培養容器において、
    上部、底部、側壁、及び首のある開口部;
    複数のマイクロキャビティを含む、前記容器の前記底部上の基板であって、前記複数のマイクロキャビティの各マイクロキャビティがウェル開口部と凹状のウェル底部とを備えている、基板;並びに
    前記基板の外周を取り囲む傾斜したフランジ
    を含み、
    前記フランジが、前記基板に隣接した内面と前記基板とは反対側の外面とを有している、
    細胞培養容器。
  2. 前記基板が前記容器の前記底部に固定されている、請求項1に記載の細胞培養容器。
  3. 前記基板が前記容器の底面と一体化している、請求項1に記載の細胞培養容器。
  4. 前記フランジの前記外面が傾斜している、請求項1から3いずれか1項に記載の細胞培養容器。
  5. 前記傾斜したフランジの外周を取り囲み、前記フランジの外側で前記基板の周りに堀を形成するチャネルをさらに含む、請求項1から4いずれか1項に記載の細胞培養容器。
  6. 請求項1に記載の細胞培養容器内で細胞を培養する方法であって、
    前記細胞培養容器の前記フランジのネック側に液体を堆積させること;
    液体が前記フランジを越えて複数のマイクロキャビティを含む前記基板上にこぼれるまで、前記容器を液体で満たすこと;及び
    前記複数のマイクロキャビティ内で細胞を培養すること
    を含む、方法。
  7. 培養後、前記細胞培養容器を傾けてチャネル内に細胞液を蓄積すること;
    前記容器の開口に回収ポートを挿入し、該回収ポートを使用して前記チャネルから液体を除去すること
    をさらに含む、請求項に記載の方法。
JP2020501517A 2017-07-14 2018-07-13 3d培養のための細胞培養容器及び3d細胞の培養方法 Active JP7195302B2 (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201762532639P 2017-07-14 2017-07-14
US201762532671P 2017-07-14 2017-07-14
US201762532648P 2017-07-14 2017-07-14
US62/532,648 2017-07-14
US62/532,639 2017-07-14
US62/532,671 2017-07-14
US201862642400P 2018-03-13 2018-03-13
US62/642,400 2018-03-13
PCT/US2018/042115 WO2019014610A1 (en) 2017-07-14 2018-07-13 CELL CULTURE CONTAINER FOR 3D CULTURE AND METHODS OF CULTURING 3D CELLS

Publications (3)

Publication Number Publication Date
JP2020526219A JP2020526219A (ja) 2020-08-31
JP2020526219A5 true JP2020526219A5 (ja) 2021-08-19
JP7195302B2 JP7195302B2 (ja) 2022-12-23

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JP2020501517A Active JP7195302B2 (ja) 2017-07-14 2018-07-13 3d培養のための細胞培養容器及び3d細胞の培養方法

Country Status (5)

Country Link
US (1) US11584906B2 (ja)
EP (1) EP3652292A1 (ja)
JP (1) JP7195302B2 (ja)
CN (1) CN111094536B (ja)
WO (1) WO2019014610A1 (ja)

Families Citing this family (2)

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EP4038176A1 (en) 2019-10-03 2022-08-10 Corning Incorporated Kit and method for preparation of digestible spheroid stabilizing hydrogels

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