JP2006314276A - 細胞培養容器 - Google Patents
細胞培養容器 Download PDFInfo
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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Abstract
【解決手段】全体として扁平な形状の容器であって、相互に所定の間隔をおいて対向する2つの平面状表面(2、2)と該2つの平面状表面を相互接続する側部(3)を備え、平面状表面(2、2)の少なくとも一つはガス透過膜(5)を有し、側部(3)の変形によって容器の内容積を変えることができる培養容器。
【選択図】図1
Description
図1は本発明に係る培養容器の1実施形態を示すもので、(a)は斜視図、(b)は図1(a)のA−A断面図、(c)は図1(a)の容器の内容積を最小値となるまで減少させた状態の斜視図である。
内溶液の充填時には、最初容器1を押し潰して内容積を最小値まで減少した状態とした後、内容液充填/取出し用栓体8から細胞、微生物等と培養液の混合液である内溶液を容器1内に充填する。内溶液の充填が進むにつれて、容器1の各側部3aと3bは、折れ線9を介して相互に接触した状態から離間し、容器1の内容積は次第に増大する。そして内溶液を設定量だけ充填すると、側部3aと3bが、所定の角度だけ相互に離間した形状で充填が終了する。この充填量は、各側部3が平坦面になる量が最大値となる。
2 平面状表面
3 側部
5 ガス透過膜
21 補強枠体
24 培養ガス封入用空間
Claims (7)
- 全体として扁平な形状の容器であって、相互に所定の間隔をおいて対向する2つの平面状表面と該2つの平面状表面を相互接続する側部を備え、該平面状表面の少なくとも一つはガス透過膜を有し、該側部の変形によって容器の内容積を変えることができることを特徴とする培養容器。
- 該側部は屈曲可能であることを特徴とする請求項1記載の培養容器。
- 該側部は伸縮可能なであることを特徴とする請求項1記載の培養容器。
- 該ガス透過膜を平面状に維持するための補強手段を備えることを特徴とする請求項1〜3のいずれかに記載の培養容器。
- 該ガス透過膜に接して培養ガス封入用空間が形成されていることを特徴とする請求項1〜4のいずれかに記載の培養容器。
- 該容器を、該平面状表面を水平方向に配置して複数個相互に所定の間隔をおいて積載し、これら積載された複数の容器を別の単一の容器内に収容し、該単一の容器内に培養ガスを封入する空間を形成したことを特徴とする請求項1〜4のいずれかに記載の培養容器。
- 該容器内を多孔質膜または半透膜により複数の部屋に分割したことを特徴とする請求項1〜6のいずれかに記載の培養容器。
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007043699A1 (ja) * | 2005-10-14 | 2007-04-19 | Toyo Seikan Kaisha, Ltd. | 培養容器及び培養方法 |
WO2014045532A1 (ja) * | 2012-09-24 | 2014-03-27 | 東洋製罐グループホールディングス株式会社 | 気泡除去方法、及び気泡除去装置 |
WO2015145954A1 (ja) * | 2014-03-28 | 2015-10-01 | 東洋製罐グループホールディングス株式会社 | 細胞培養方法、及び細胞培養システム |
JP2017195857A (ja) * | 2016-04-28 | 2017-11-02 | 株式会社京都製作所 | 培養バッグおよび培養装置 |
WO2020067434A1 (ja) * | 2018-09-27 | 2020-04-02 | テルモ株式会社 | 細胞の凍結保存方法 |
JP2020054392A (ja) * | 2016-04-28 | 2020-04-09 | 株式会社京都製作所 | 培養バッグおよび培養装置 |
JP2020080670A (ja) * | 2018-11-17 | 2020-06-04 | 東洋製罐グループホールディングス株式会社 | 培地充填液、培地充填方法、培養容器、及び培地充填用気泡除去装置 |
AU2017357650B2 (en) * | 2016-11-11 | 2023-07-13 | Oribiotech Ltd | Cell culture device system and methods of use thereof |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0613499U (ja) * | 1992-07-24 | 1994-02-22 | 積水化学工業株式会社 | 培養容器 |
JPH07155170A (ja) * | 1993-12-07 | 1995-06-20 | Fujimori Kogyo Kk | 微生物、植物または動物の振盪培養用具および培養方法 |
JP2004129568A (ja) * | 2002-10-10 | 2004-04-30 | Olympus Corp | 培養容器および培養方法 |
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2005
- 2005-05-13 JP JP2005141331A patent/JP4706327B2/ja active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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JPH0613499U (ja) * | 1992-07-24 | 1994-02-22 | 積水化学工業株式会社 | 培養容器 |
JPH07155170A (ja) * | 1993-12-07 | 1995-06-20 | Fujimori Kogyo Kk | 微生物、植物または動物の振盪培養用具および培養方法 |
JP2004129568A (ja) * | 2002-10-10 | 2004-04-30 | Olympus Corp | 培養容器および培養方法 |
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007043699A1 (ja) * | 2005-10-14 | 2007-04-19 | Toyo Seikan Kaisha, Ltd. | 培養容器及び培養方法 |
WO2014045532A1 (ja) * | 2012-09-24 | 2014-03-27 | 東洋製罐グループホールディングス株式会社 | 気泡除去方法、及び気泡除去装置 |
JP2014064476A (ja) * | 2012-09-24 | 2014-04-17 | Toyo Seikan Group Holdings Ltd | 気泡除去方法、及び気泡除去装置 |
US10077124B2 (en) | 2012-09-24 | 2018-09-18 | Toyo Seikan Group Holdings, Ltd. | Bubble removal method and bubble removal device |
JP2015188392A (ja) * | 2014-03-28 | 2015-11-02 | 東洋製罐グループホールディングス株式会社 | 細胞培養方法、及び細胞培養システム |
KR20160124852A (ko) * | 2014-03-28 | 2016-10-28 | 도요세이칸 그룹 홀딩스 가부시키가이샤 | 세포 배양 방법 및 세포 배양 시스템 |
CN106103684A (zh) * | 2014-03-28 | 2016-11-09 | 东洋制罐集团控股株式会社 | 细胞培养方法、以及细胞培养系统 |
WO2015145954A1 (ja) * | 2014-03-28 | 2015-10-01 | 東洋製罐グループホールディングス株式会社 | 細胞培養方法、及び細胞培養システム |
KR101925778B1 (ko) * | 2014-03-28 | 2019-02-26 | 도요세이칸 그룹 홀딩스 가부시키가이샤 | 세포 배양 방법 및 세포 배양 시스템 |
JP2017195857A (ja) * | 2016-04-28 | 2017-11-02 | 株式会社京都製作所 | 培養バッグおよび培養装置 |
JP2020054392A (ja) * | 2016-04-28 | 2020-04-09 | 株式会社京都製作所 | 培養バッグおよび培養装置 |
AU2017357650B2 (en) * | 2016-11-11 | 2023-07-13 | Oribiotech Ltd | Cell culture device system and methods of use thereof |
US11993765B2 (en) * | 2016-11-11 | 2024-05-28 | Silicon Valley Bank Uk Limited | Cell culture device system and methods of use thereof |
WO2020067434A1 (ja) * | 2018-09-27 | 2020-04-02 | テルモ株式会社 | 細胞の凍結保存方法 |
JP2020080670A (ja) * | 2018-11-17 | 2020-06-04 | 東洋製罐グループホールディングス株式会社 | 培地充填液、培地充填方法、培養容器、及び培地充填用気泡除去装置 |
JP7322384B2 (ja) | 2018-11-17 | 2023-08-08 | 東洋製罐グループホールディングス株式会社 | 培地充填液、培地充填方法、培養容器、及び培地充填用気泡除去装置 |
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Free format text: JAPANESE INTERMEDIATE CODE: R250 |
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R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
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R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |