JP2020504849A - 核酸に基づくデータ保存 - Google Patents
核酸に基づくデータ保存 Download PDFInfo
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- JP2020504849A JP2020504849A JP2019515660A JP2019515660A JP2020504849A JP 2020504849 A JP2020504849 A JP 2020504849A JP 2019515660 A JP2019515660 A JP 2019515660A JP 2019515660 A JP2019515660 A JP 2019515660A JP 2020504849 A JP2020504849 A JP 2020504849A
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- polynucleotides
- polynucleotide
- sequence
- nucleic acid
- information
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Abstract
Description
本出願は、2017年6月9日出願の米国仮特許出願第62/517,671号;2017年1月13日出願の米国仮特許出願第62/446,178号;および2016年9月21日出願の米国仮特許出願第62/397,855号の利益を主張し、その各々は、その全体が参照により本明細書に組み込まれる。
本出願は、ASCIIフォーマットで電子的に提出され、参照によりその全体が本明細書に組み込まれる配列表を含んでいる。2017年9月18日に作成されたASCIIのコピーは、44854_728_601_SL.txtのファイル名であり、1,612バイトのサイズである。
本明細書で言及される全ての刊行物、特許、および特許出願は、個々の刊行物、特許、または特許出願が参照により組み込まれるように、具体的かつ個々に示されているかのような程度で、参照により本明細書に組み込まれる。
様々な態様では、本明細書に記載されるシステムのいずれかが、コンピュータに動作可能に接続され、および随意に、ローカルまたは遠隔のいずれかでコンピュータを介して自動化される。様々な実施形態では、本発明の方法とシステムはさらに、コンピュータシステムのソフトウェアプログラムとその使用を含む。したがって、材料沈着デバイスの動き、分配動作、および真空発動の組織化と同期等の、分配/真空/再補充機能の同期のためのコンピュータ制御は、本発明の範囲内にある。いくつかの例では、コンピュータシステムは、ユーザーに特異的な塩基配列と、基質の所定領域に適正な試薬を送達するための材料沈着デバイスの位置をインターフェースで接続するようにプログラムされる。
Claims (75)
- 情報を保存し、および情報にアクセスするための方法であって、該方法は:
a)少なくとも1つのデジタル配列の形態の情報の少なくとも1項目を、少なくとも1つの核酸配列に変換する工程;
b)面を含む構造を提供する工程;
c)共同して少なくとも1つの核酸配列をコードする、あらかじめ決定された配列を有する複数のポリヌクレオチドを合成する工程であって、各ポリヌクレオチドは面から伸長する、工程;
d)複数のポリヌクレオチドを保存する工程;および
e)受信ユニットに複数のポリヌクレオチドを選択的に転写する工程であって、選択的に転写する工程は力の適用を含み、該力は層状の圧力、毛細血管圧、すべり流圧、磁力、静電力、ぜん動力、音波、震動力、向心力、遠心力、またはそれらの任意の組み合わせであり、および複数のポリヌクレオチドは共同して少なくとも1つの核酸配列の単一の核酸配列をコードする、工程、
を含む、方法。 - 力の適用は、伝導性部材と、構造と伝導性部材の間に適用されたボルテージポテンシャルを含む、請求項1に記載の方法。
- 力の適用は、構造の面を固いまたは柔らかい滑りに接触させる工程を含む、請求項1に記載の方法。
- 力の適用は、圧力解放または圧力ノズルを含む、請求項1に記載の方法。
- 工程(c)の間に圧力ノズルを使用する工程をさらに含む、請求項4に記載の方法。
- 圧力ノズルを通じてポリヌクレオチドを充満させる工程をさらに含む、請求項4に記載の方法。
- 圧力ノズルを通じてヌクレオチドを沈着させる工程をさらに含む、請求項4に記載の方法。
- 複数のポリヌクレオチドを配列する工程;および
少なくとも1つのデジタル配列をアセンブルする工程、
をさらに含む、請求項1に記載の方法。 - アセンブルした少なくとも1つのデジタル配列は、最初の少なくとも1つのデジタル配列と比較して100%の精度である、請求項8に記載の方法。
- 情報を保存するための方法であって、該方法は:
a)少なくとも1つのデジタル配列の形態の情報の少なくとも1項目を、少なくとも1つの核酸配列に変換する工程;
b)共同して少なくとも1つの核酸配列をコードする、あらかじめ決定された配列を有する複数のポリヌクレオチドを合成する工程であって、各ポリヌクレオチドは;
i)各コード領域が同一である、複数のコード領域;および
ii)切断領域を含む、少なくとも1つの非コード領域、
を含む、工程;および
c)複数のポリヌクレオチドを保存する工程、
を含む、方法。 - 切断領域は制限酵素認識部位を含む、請求項10に記載の方法。
- 切断領域は光に感受性のある核酸塩基を含む、請求項10に記載の方法。
- 切断領域での切断への、制限酵素、電磁放射線またはガス状試薬の適用をさらに含み、それによって複数のコード領域の少なくとも1つを除去する、請求項10に記載の方法。
- 各コード領域は25〜500塩基の長さを含む、請求項10に記載の方法。
- 各コード領域は100〜2000塩基の長さを含む、請求項10に記載の方法。
- 各非コード領域は1〜100塩基の長さを含む、請求項10に記載の方法。
- 各非コード領域は最大で200の塩基を含む、請求項10に記載の方法。
- 複数のポリヌクレオチドは少なくとも100,000のポリヌクレオチドを含む、請求項10に記載の方法。
- 複数のポリヌクレオチドは少なくとも100億のポリヌクレオチドを含む、請求項10に記載の方法。
- ポリヌクレオチドの90%より多くが、あらかじめ決定された配列と差異のない配列をコードする、請求項10に記載の方法。
- 情報の少なくとも1項目は、テキスト情報、オーディオ情報またはビジュアル情報である、請求項10に記載の方法。
- 各ポリヌクレオチド内の第1の非コード領域は、各ポリヌクレオチド内の第2の非コード領域とは異なる配列を有する、請求項10に記載の方法。
- 各ポリヌクレオチド内の各非コード領域は異なる配列を有する、請求項10に記載の方法。
- 各ポリヌクレオチド内の第1の切断領域は、各ポリヌクレオチド内の第2の切断領域とは異なる配列を有する、請求項10に記載の方法。
- 各ポリヌクレオチド内の各切断領域は異なる配列を有する、請求項10に記載の方法。
- 各ポリヌクレオチド内の切断領域の数は、少なくとも1、2、3、4、または5である、請求項10に記載の方法。
- 切断領域の数に対する配列は異なっている、請求項26に記載の方法。
- 各ポリヌクレオチドはテザー領域を含む、請求項10に記載の方法。
- 情報を暗号化するための方法であって、該方法は:
a)少なくとも1つのデジタル配列の形態の情報の少なくとも1項目を、少なくとも1つの核酸配列に変換する工程;
b)少なくとも1つの核酸配列の各々を、複数の非同一のマーキングの1つと関連付ける工程;
c)面を有する構造を提供する工程であって、面は複数の非同一のマーキングを含む、工程;
d)少なくとも1つの核酸配列を共同してコードするあらかじめ決定された配列を有する複数のポリヌクレオチドを合成する工程であって、複数のポリヌクレオチドは少なくとも100,000のポリヌクレオチドを含み、および各ポリヌクレオチドは、非同一のマーキングの1つによって分離される分離領域の面から伸長する、工程;および
e)複数のポリヌクレオチドを保存する工程、
を含む、方法。 - 複数のポリヌクレオチドは、少なくとも1,000,000のポリヌクレオチドを含む、請求項29に記載の方法。
- ポリヌクレオチドの90%より多くが、あらかじめ決定された配列と差異のない配列をコードする、請求項29に方法。
- 情報の少なくとも1項目は、テキスト情報、オーディオ情報またはビジュアル情報である、請求項29に記載の方法。
- 非同一のマーキングの1つによって別々に分離されたポリヌクレオチドのサブセットは、同じ配列を含む、請求項29に記載の方法。
- 非同一のマーキングの1つによって別々に分離されたポリヌクレオチドのサブセットを選択する工程、ポリヌクレオチドのサブセットを放出する工程、複数のポリヌクレオチドを配列する工程、複数のポリヌクレオチドを解読する工程、および少なくとも1つのデジタル配列をアセンブルする工程、をさらに含む、請求項29に記載の方法。
- 非同一のマーキングの1つによって別々に分離されたポリヌクレオチドのサブセットを選択する工程、ポリヌクレオチドのサブセットを増幅する工程、ポリヌクレオチドのサブセットを配列する工程、複数のポリヌクレオチドを解読する工程、および少なくとも1つのデジタル配列をアセンブルする工程、をさらに含む、請求項29に記載の方法。
- アセンブルした少なくとも1つのデジタル配列は、最初の少なくとも1つのデジタル配列と比較して100%の精度である、請求項34または35に記載の方法。
- 少なくとも1つのデジタル配列は、少なくとも1ギガバイトのデジタル情報量を含む、請求項35に記載の方法。
- 少なくとも1つのデジタル配列は、少なくとも1テラバイトのデジタル情報量を含む、請求項35に記載の方法。
- 少なくとも1つのデジタル配列は、少なくとも1ペタバイトのデジタル情報量を含む、請求項35に記載の方法。
- 情報を収集するための方法であって、該方法は:
a)面を含む構造を提供する工程であって、該構造は:
少なくとも1つの核酸配列を共同してコードする、あらかじめ決定された配列を有する第1の複数のポリヌクレオチド;および少なくとも1つの核酸配列を共同してコードする、あらかじめ決定された配列を有する第2の複数のポリヌクレオチド
を含み、ここで第1の複数のポリヌクレオチドと第2の複数のポリヌクレオチドの両方が面から伸長し、同じ少なくとも1つの核酸配列を共にコードする、工程;
b)第1の複数のポリヌクレオチドを含む構造の領域を選択的に分離し、および面から第1の複数のポリヌクレオチドを除去する工程;および
c)情報の1項目をコードする少なくとも1つのデジタル配列を形成するために、少なくとも1つのデジタル配列を配列し、かつ解読する工程、
を含む、方法。 - 第1の複数のポリヌクレオチドを含む構造の領域は、チャネルまたはウェルのクラスタを含む、請求項40に記載の方法。
- 構造は固い構造である、請求項40に記載の方法。
- 構造は柔らかい構造である、請求項40に記載の方法。
- 第1の複数のポリヌクレオチドを欠いている構造の残りの部分のみを含む構造の領域は、元通りになるよう一緒にスプライシングされる、請求項40に記載の方法。
- 選択的に除去する工程は、第1の複数のポリヌクレオチドを含む構造の領域への力の適用を含む、請求項40に記載の方法。
- 力の適用は、層状の圧力、毛細血管圧、すべり流圧、磁力、静電力、ぜん動力、音波、震動力、向心力、遠心力、またはそれらの任意の組み合わせである、請求項45に記載の方法。
- 力の適用は、伝導性部材と、構造と伝導性部材の間に適用されたボルテージポテンシャルを含む、請求項45に記載の方法。
- 力の適用は、構造の面を固いまたは柔らかい滑りに接触させる工程を含む、請求項45に記載の方法。
- 力の適用は、圧力解放または圧力ノズルを含む、請求項45に記載の方法。
- 第1の複数のヌクレオチドの各ポリヌクレオチドは、最大で500塩基の長さを含む、請求項40に記載の方法。
- 第1の複数のヌクレオチドの各ポリヌクレオチドは、最大で200塩基の長さを含む、請求項40に記載の方法。
- 第2の複数のヌクレオチドの各ポリヌクレオチドは、最大で500塩基の長さを含む、請求項40に記載の方法。
- 第2の複数のヌクレオチドの各ポリヌクレオチドは、最大で200塩基の長さを含む、請求項40に記載の方法。
- 情報の項目の量は少なくとも1ギガバイトである、請求項40に記載の方法。
- 情報の項目の量は少なくとも1テラバイトである、請求項40に記載の方法。
- 情報の項目の量は少なくとも1ペタバイトである、請求項40に記載の方法。
- 複数のポリヌクレオチドを含む核酸ライブラリであって、ポリヌクレオチドの各々は:
i)各コード領域が同一である、複数のコード領域;および
ii)切断領域を含む、少なくとも1つの非コード領域
を含み、およびデジタル配列を形成するために複数のポリヌクレオチドが配列され、解読され、アセンブルされる場合、デジタル配列は、あらかじめ選択されたデジタル配列と比較して90%を超える精度を有する、核酸ライブラリ。 - 切断領域は制限酵素認識部位を含む、請求項57に記載の核酸ライブラリ。
- 切断領域は光に感受性のある核酸塩基を含む、請求項57に記載の核酸ライブラリ。
- 切断領域での切断への、制限酵素、電磁放射線またはガス状試薬の適用をさらに含み、それによって複数のコード領域の少なくとも1つを除去する、請求項57に記載の核酸ライブラリ。
- 各コード領域は、25〜500塩基の長さを含む、請求項57に記載の核酸ライブラリ。
- 各コード領域は、100〜2000塩基の長さを含む、請求項57に記載の核酸ライブラリ。
- 各非コード領域は、1〜100塩基の長さを含む、請求項57に記載の核酸ライブラリ。
- 各非コード領域は、多くとも200塩基を含む、請求項57に記載の核酸ライブラリ。
- 複数のポリヌクレオチドは少なくとも100,000のポリヌクレオチドを含む、請求項57に記載の核酸ライブラリ。
- 複数のポリヌクレオチドは少なくとも100億のポリヌクレオチドを含む、請求項57に記載の核酸ライブラリ。
- ポリヌクレオチドの90%より多くが、あらかじめ決定された配列と差異のない配列をコードする、請求項57に記載の核酸ライブラリ。
- 各ポリヌクレオチド内の第1の非コード領域は、各ポリヌクレオチド内の第2の非コード領域とは異なる配列を有する、請求項57に記載の核酸ライブラリ。
- 各ポリヌクレオチド内の各非コード領域は異なる配列を有する、請求項57に記載の核酸ライブラリ。
- 各ポリヌクレオチド内の第1の切断領域は、各ポリヌクレオチド内の第2の切断領域とは異なる配列を有する、請求項57に記載の核酸ライブラリ。
- 各ポリヌクレオチド内の各切断領域は異なる配列を有する、請求項57に記載の核酸ライブラリ。
- 各ポリヌクレオチド内の切断領域の数は、少なくとも1、2、3、4、または5である、請求項57に記載の核酸ライブラリ。
- 切断領域の数に対する配列は異なっている、請求項72に記載の核酸ライブラリ。
- 情報を保存するためのデバイスであって、該デバイスは:
a)面を有する構造;および
b)少なくとも1つの核酸配列を共同してコードするあらかじめ決定された配列を有する複数のポリヌクレオチドを合成するための、面上の複数の分離領域であって、各ポリヌクレオチドは:
i)各コード領域が同一である、複数のコード領域;および
ii)切断領域を含む、少なくとも1つの非コード領域、
を含む、分離領域;
を含み、および少なくとも1つの核酸配列は、情報の少なくとも1項目をコードする、デバイス。 - 情報を暗号化するためのデバイスであって、該デバイスは:
a)面を有する構造であって、面は複数の非同一のマーキングを含む、構造;
b)少なくとも1つの核酸配列を共同してコードするあらかじめ決定された配列を有する複数のポリヌクレオチドを合成するための、面上の複数の分離領域であって、複数のポリヌクレオチドは少なくとも100,000のポリヌクレオチドを含み、および各ポリヌクレオチドは、非同一のマーキングの1つによって分離される分離領域の面から伸長する、分離領域;
を含み、および少なくとも1つの核酸配列は、情報の少なくとも1項目をコードする、デバイス。
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KR20190049885A (ko) | 2019-05-09 |
KR102217487B1 (ko) | 2021-02-23 |
CN110248724B (zh) | 2022-11-18 |
US20190318132A1 (en) | 2019-10-17 |
EP3516528A2 (en) | 2019-07-31 |
WO2018057526A3 (en) | 2019-05-23 |
US20200342143A1 (en) | 2020-10-29 |
US20180101487A1 (en) | 2018-04-12 |
US10417457B2 (en) | 2019-09-17 |
US10754994B2 (en) | 2020-08-25 |
US11562103B2 (en) | 2023-01-24 |
US11263354B2 (en) | 2022-03-01 |
US20230185971A1 (en) | 2023-06-15 |
JP6871364B2 (ja) | 2021-05-12 |
US20220138354A1 (en) | 2022-05-05 |
CN110248724A (zh) | 2019-09-17 |
US20230054232A1 (en) | 2023-02-23 |
WO2018057526A2 (en) | 2018-03-29 |
EP3516528A4 (en) | 2020-06-24 |
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