JP2015503907A5 - - Google Patents

Download PDF

Info

Publication number
JP2015503907A5
JP2015503907A5 JP2014547977A JP2014547977A JP2015503907A5 JP 2015503907 A5 JP2015503907 A5 JP 2015503907A5 JP 2014547977 A JP2014547977 A JP 2014547977A JP 2014547977 A JP2014547977 A JP 2014547977A JP 2015503907 A5 JP2015503907 A5 JP 2015503907A5
Authority
JP
Japan
Prior art keywords
heavy chain
antibody
nucleic acid
antigen
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
JP2014547977A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015503907A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/EP2012/076163 external-priority patent/WO2013092720A1/en
Publication of JP2015503907A publication Critical patent/JP2015503907A/ja
Publication of JP2015503907A5 publication Critical patent/JP2015503907A5/ja
Ceased legal-status Critical Current

Links

JP2014547977A 2011-12-22 2012-12-19 真核細胞のための全長抗体提示システムおよびその使用 Ceased JP2015503907A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP11195375.8 2011-12-22
EP11195375 2011-12-22
EP12179029 2012-08-02
EP12179029.9 2012-08-02
PCT/EP2012/076163 WO2013092720A1 (en) 2011-12-22 2012-12-19 Full length antibody display system for eukaryotic cells and its use

Publications (2)

Publication Number Publication Date
JP2015503907A JP2015503907A (ja) 2015-02-05
JP2015503907A5 true JP2015503907A5 (enExample) 2016-02-12

Family

ID=47458959

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014547977A Ceased JP2015503907A (ja) 2011-12-22 2012-12-19 真核細胞のための全長抗体提示システムおよびその使用

Country Status (12)

Country Link
US (1) US20150038354A1 (enExample)
EP (1) EP2794662A1 (enExample)
JP (1) JP2015503907A (enExample)
KR (1) KR20140107295A (enExample)
CN (1) CN104011080B (enExample)
BR (1) BR112014013035A2 (enExample)
CA (1) CA2854246A1 (enExample)
HK (1) HK1200849A1 (enExample)
MX (1) MX2014007262A (enExample)
RU (1) RU2625033C2 (enExample)
SG (1) SG11201403445YA (enExample)
WO (1) WO2013092720A1 (enExample)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2580017C2 (ru) 2010-07-26 2016-04-10 Трианни, Инк. Трансгенные животные и способы применения
US10662256B2 (en) 2010-07-26 2020-05-26 Trianni, Inc. Transgenic mammals and methods of use thereof
US10793829B2 (en) 2010-07-26 2020-10-06 Trianni, Inc. Transgenic mammals and methods of use thereof
GB201407852D0 (en) * 2014-05-02 2014-06-18 Iontas Ltd Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules
CN107207371A (zh) * 2014-10-20 2017-09-26 斯克利普斯研究院 用于选择结合配偶体的基于邻近度的方法
CN104818295A (zh) * 2015-02-03 2015-08-05 武汉友芝友生物制药有限公司 制备和筛选表达双特异性抗体细胞株的方法
WO2017095939A1 (en) 2015-12-03 2017-06-08 Trianni, Inc. Enhanced immunoglobulin diversity
WO2017136734A1 (en) * 2016-02-04 2017-08-10 Trianni, Inc. Enhanced production of immunoglobulins
SG10202104509PA (en) * 2016-03-19 2021-06-29 Exuma Biotech Corp Methods and compositions for transducing lymphocytes and regulated expansion thereof
US11111505B2 (en) 2016-03-19 2021-09-07 Exuma Biotech, Corp. Methods and compositions for transducing lymphocytes and regulating the activity thereof
US11325948B2 (en) 2016-03-19 2022-05-10 Exuma Biotech Corp. Methods and compositions for genetically modifying lymphocytes to express polypeptides comprising the intracellular domain of MPL
CN109790214B (zh) 2016-09-29 2022-09-06 豪夫迈·罗氏有限公司 用于选择产生多肽的细胞的改进方法
EP3526257A2 (en) * 2016-10-14 2019-08-21 Dana-Farber Cancer Institute, Inc. Modular tetravalent bispecific antibody platform
GB201720351D0 (en) 2017-12-06 2018-01-17 Iontas Ltd Selecting for developability in drug discovery
CN110872356B (zh) * 2018-09-03 2023-06-13 广西慧宝源健康产业有限公司 双特异性抗体及其使用方法
WO2020177719A1 (zh) * 2019-03-05 2020-09-10 信达生物制药(苏州)有限公司 展示与分泌目的多肽的酵母展示系统及其用途
WO2021057726A1 (zh) * 2019-09-23 2021-04-01 南开大学 利用哺乳动物展示筛选FcγR特异性结合Fc
US20230331871A1 (en) * 2020-03-27 2023-10-19 Ddbio.Co, Ltd., (Shang Hai) Method for displaying bispecific antibody on surface of mammalian cell and vector
CN113150122B (zh) * 2021-05-19 2023-03-17 上海儒克生物科技有限公司 高通量全兔源单克隆抗体的制备方法
CN118696055A (zh) * 2021-12-23 2024-09-24 赫尔辛基大学 筛选用于在哺乳动物细胞中有效表达和分泌异源多肽的信号肽的方法
WO2023238949A1 (ja) 2022-06-10 2023-12-14 富士フイルム株式会社 細胞の作出方法、ヘテロ多量体タンパク質の製造方法、バイスペシフィック抗体の製造方法、ベクターセット、哺乳動物細胞、ｃｈｏ細胞、及び細胞プールの作出方法
CN119574886A (zh) * 2024-11-29 2025-03-07 苏州泓迅生物科技股份有限公司 一种基于动物细胞表面展示库的抗体筛选方法及应用

Family Cites Families (103)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4676980A (en) 1985-09-23 1987-06-30 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Target specific cross-linked heteroantibodies
US6548640B1 (en) 1986-03-27 2003-04-15 Btg International Limited Altered antibodies
US5959177A (en) 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
US6150584A (en) 1990-01-12 2000-11-21 Abgenix, Inc. Human antibodies derived from immunized xenomice
US6075181A (en) 1990-01-12 2000-06-13 Abgenix, Inc. Human antibodies derived from immunized xenomice
US5770429A (en) 1990-08-29 1998-06-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
WO1992009690A2 (en) 1990-12-03 1992-06-11 Genentech, Inc. Enrichment method for variant proteins with altered binding properties
EP0590058B1 (en) 1991-06-14 2003-11-26 Genentech, Inc. HUMANIZED Heregulin ANTIBODy
WO1993006217A1 (en) 1991-09-19 1993-04-01 Genentech, Inc. EXPRESSION IN E. COLI OF ANTIBODY FRAGMENTS HAVING AT LEAST A CYSTEINE PRESENT AS A FREE THIOL, USE FOR THE PRODUCTION OF BIFUNCTIONAL F(ab')2 ANTIBODIES
WO1993008829A1 (en) 1991-11-04 1993-05-13 The Regents Of The University Of California Compositions that mediate killing of hiv-infected cells
US5789199A (en) 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
US5840523A (en) 1995-03-01 1998-11-24 Genetech, Inc. Methods and compositions for secretion of heterologous polypeptides
US5731168A (en) 1995-03-01 1998-03-24 Genentech, Inc. Method for making heteromultimeric polypeptides
GB9603256D0 (en) 1996-02-16 1996-04-17 Wellcome Found Antibodies
US5888809A (en) 1997-05-01 1999-03-30 Icos Corporation Hamster EF-1α transcriptional regulatory DNA
EP0979281B1 (en) * 1997-05-02 2005-07-20 Genentech, Inc. A method for making multispecific antibodies having heteromultimeric and common components
EP0994903B1 (en) 1997-06-24 2005-05-25 Genentech, Inc. Methods and compositions for galactosylated glycoproteins
US6040498A (en) 1998-08-11 2000-03-21 North Caroline State University Genetically engineered duckweed
AU759779B2 (en) 1997-10-31 2003-05-01 Genentech Inc. Methods and compositions comprising glycoprotein glycoforms
US6610833B1 (en) 1997-11-24 2003-08-26 The Institute For Human Genetics And Biochemistry Monoclonal human natural antibodies
JP4460155B2 (ja) 1997-12-05 2010-05-12 ザ・スクリプス・リサーチ・インステイチユート マウス抗体のヒト化
JP4334141B2 (ja) 1998-04-20 2009-09-30 グリカート バイオテクノロジー アクチェンゲゼルシャフト 抗体依存性細胞傷害性を改善するための抗体のグリコシル化操作
CA2369292C (en) 1999-04-09 2010-09-21 Kyowa Hakko Kogyo Co. Ltd. Method of modulating the activity of functional immune molecules
JP2003512821A (ja) 1999-10-04 2003-04-08 メディカゴ インコーポレイテッド 外来性遺伝子の転写調節方法
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
WO2001029246A1 (fr) 1999-10-19 2001-04-26 Kyowa Hakko Kogyo Co., Ltd. Procede de production d'un polypeptide
US20030180714A1 (en) 1999-12-15 2003-09-25 Genentech, Inc. Shotgun scanning
DK1272647T3 (en) 2000-04-11 2014-12-15 Genentech Inc Multivalent antibodies and uses thereof
US6627442B1 (en) * 2000-08-31 2003-09-30 Virxsys Corporation Methods for stable transduction of cells with hiv-derived viral vectors
EA013563B1 (ru) 2000-10-06 2010-06-30 Киова Хакко Кирин Ко., Лтд. Трансгенное животное, продуцирующее антитела с измененными углеводными цепями, способ получения антител и содержащее антитела лекарственное средство
US6946292B2 (en) 2000-10-06 2005-09-20 Kyowa Hakko Kogyo Co., Ltd. Cells producing antibody compositions with increased antibody dependent cytotoxic activity
US7064191B2 (en) 2000-10-06 2006-06-20 Kyowa Hakko Kogyo Co., Ltd. Process for purifying antibody
US6596541B2 (en) 2000-10-31 2003-07-22 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
AU2001297872B2 (en) 2000-11-17 2006-11-09 University Of Rochester In vitro methods of producing and identifying immunoglobulin molecules in eukaryotic cells
DK1354034T3 (da) 2000-11-30 2008-03-25 Medarex Inc Transgene transchromosomale gnavere til fremstilling af humane antistoffer
CA2455365C (en) 2001-08-03 2014-07-29 Glycart Biotechnology Ag Antibody glycosylation variants having increased antibody-dependent cellular cytotoxicity
ATE447967T1 (de) 2001-09-14 2009-11-15 Cytos Biotechnology Ag Verpackung von immunstimulierendem cpg in virusähnlichen partikeln: herstellungsverfahren und verwendung
AU2002362696B2 (en) 2001-10-05 2008-05-29 Cytos Biotechnology Ag Angiotensin peptide-carrier conjugates and uses thereof
ATE430580T1 (de) 2001-10-25 2009-05-15 Genentech Inc Glycoprotein-zusammensetzungen
PL369863A1 (en) 2001-11-07 2005-05-02 Cytos Biotechnology Ag Antigen arrays for treatment of allergic eosinophilic diseases
KR20050044316A (ko) 2001-11-07 2005-05-12 사이토스 바이오테크놀로지 아게 호산구성 알레르기 질환 치료용의 ｉｌ-5, ｉｌ-13 또는에오탁신을 제시하는 항원 어레이
EP2022799A2 (en) * 2001-11-16 2009-02-11 Biogen Idec Inc. Polycistronic expression of antibodies
US20040093621A1 (en) 2001-12-25 2004-05-13 Kyowa Hakko Kogyo Co., Ltd Antibody composition which specifically binds to CD20
CN1429916A (zh) * 2001-12-30 2003-07-16 韩泽广 规模化原核和真核细胞体内抗体和抗原文库构建及筛选
CN1930288B (zh) 2002-04-09 2012-08-08 协和发酵麒麟株式会社 基因组被修饰的细胞
WO2003085119A1 (en) 2002-04-09 2003-10-16 Kyowa Hakko Kogyo Co., Ltd. METHOD OF ENHANCING ACTIVITY OF ANTIBODY COMPOSITION OF BINDING TO FcϜ RECEPTOR IIIa
JPWO2003085118A1 (ja) 2002-04-09 2005-08-11 協和醗酵工業株式会社 抗体組成物の製造方法
ES2362419T3 (es) 2002-04-09 2011-07-05 Kyowa Hakko Kirin Co., Ltd. Células con depresión o deleción de la actividad de la proteína que participa en el transporte de gdp-fucosa.
CA2481920A1 (en) 2002-04-09 2003-10-16 Kyowa Hakko Kogyo Co., Ltd. Antibody composition-containing medicament
WO2003084570A1 (en) 2002-04-09 2003-10-16 Kyowa Hakko Kogyo Co., Ltd. DRUG CONTAINING ANTIBODY COMPOSITION APPROPRIATE FOR PATIENT SUFFERING FROM FcϜRIIIa POLYMORPHISM
CA2488441C (en) 2002-06-03 2015-01-27 Genentech, Inc. Synthetic antibody phage libraries
US20050112095A1 (en) * 2002-07-09 2005-05-26 Tsu-An Hsu Internal ribosome entry sites for recombinant protein expression
RU2324704C2 (ru) 2002-07-17 2008-05-20 Цитос Байотекнолоджи Аг Молекулярные массивы антигенов
EP2392345A3 (en) 2002-07-18 2012-03-07 Cytos Biotechnology AG Hapten-carrier conjugates comprising virus like particles and uses thereof
WO2004009124A2 (en) 2002-07-19 2004-01-29 Cytos Biotechnology Ag Ghrelin-carrier conjugates
RU2450827C2 (ru) 2002-07-19 2012-05-20 Цитос Биотехнологи Аг Композиции вакцин, содержащие наборы антигенов в виде амилоида бета 1-6
PT1572744E (pt) 2002-12-16 2010-09-07 Genentech Inc Variantes de imunoglobulina e utilizações destas
US20050079574A1 (en) 2003-01-16 2005-04-14 Genentech, Inc. Synthetic antibody phage libraries
KR101518309B1 (ko) * 2003-03-20 2015-05-08 알파벡스, 인크. 개선된 알파바이러스 레플리콘 및 헬퍼 구축물
JP5022028B2 (ja) 2003-03-26 2012-09-12 サイトス・バイオテクノロジー・アクチェンゲゼルシャフト メラン−ａペプチドアナログ−ウイルス様粒子コンジュゲート
WO2004096272A2 (en) 2003-04-29 2004-11-11 Cytos Biotechnology Ag Methods and compositions for modulating the interaction between adiponectin and its receptor
US20070087331A1 (en) 2003-05-15 2007-04-19 Cytos Biotechnology Ag Selection of b cells with specificity if interest: method of preparation and use
EP2395017A3 (en) * 2003-05-30 2012-12-19 Merus B.V. Design and use of paired variable regions of specific binding molecules
CN1289668C (zh) * 2003-06-12 2006-12-13 北京安波特基因工程技术有限公司 一种用于抗体改形的体外分子定向进化方法
CA2527102A1 (en) 2003-07-10 2005-01-20 Cytos Biotechnology Ag Packaged virus-like particles
MXPA06000562A (es) 2003-07-15 2006-03-30 Therapeutic Human Polyclonals Loci de inmunoglobulina humanizada.
AU2004279742A1 (en) 2003-10-08 2005-04-21 Kyowa Hakko Kirin Co., Ltd. Fused protein composition
EP1705251A4 (en) 2003-10-09 2009-10-28 Kyowa Hakko Kirin Co Ltd PROCESS FOR PRODUCING ANTIBODY COMPOSITION BY RNA INHIBITION OF FUNCTION OF $ G (A) 1,6-FUCOSYLTRANSFERASE
ME01775B (me) 2003-11-05 2011-02-28 Glycart Biotechnology Ag Cd20 antitijela sa povećanim afinitetom vezivanja za fc receptor i efektornom funkcijom
JPWO2005053742A1 (ja) 2003-12-04 2007-06-28 協和醗酵工業株式会社 抗体組成物を含有する医薬
WO2005068639A2 (en) 2004-01-20 2005-07-28 Cytos Biotechnology Ag Particle-induced ghrelin immune response
CA2561686C (en) 2004-03-31 2014-12-02 Genentech, Inc. Humanized anti-tgf-beta antibodies
US7785903B2 (en) 2004-04-09 2010-08-31 Genentech, Inc. Variable domain library and uses
WO2005108425A1 (en) 2004-05-10 2005-11-17 Cytos Biotechnology Ag Il-23 p19 antigen array and uses thereof
RU2006146605A (ru) 2004-06-02 2008-07-20 Цитос Биотехнологи Аг (Ch) Медицинские применения конъюгатов-носителей tnf-пептидов из организма кроме человека
US20080019991A1 (en) 2004-06-02 2008-01-24 Cytos Biotechnology Ag Carrier Conjugates Of Tnf-Peptides
US20090028886A1 (en) 2004-08-04 2009-01-29 Cytos Biotechnology Ag Carrier conjugates of gnrh-peptides
BRPI0516953A (pt) 2004-09-21 2008-09-30 Cytos Biotechnology Ag partìculas do tipo vìrus compreendendo uma proteìna de fusão da proteìna de revestimento de ap205 e um polipeptìdeo antigênico
CA2580919A1 (en) 2004-10-25 2006-05-04 Cytos Biotechnology Ag Gastric inhibitory polypeptide (gip) antigen arrays and uses thereof
JP2008523132A (ja) 2004-12-13 2008-07-03 サイトス バイオテクノロジー アーゲー Ｉｌ−１５抗原アレイとその使用法
KR20070112225A (ko) 2005-03-18 2007-11-22 사이토스 바이오테크놀로지 아게 고양이 알레르겐 컨쥬게이트 및 그의 용도
CA2625619A1 (en) 2005-10-14 2007-04-26 Medimmune, Inc. Cell display of antibody libraries
EP2465870A1 (en) 2005-11-07 2012-06-20 Genentech, Inc. Binding polypeptides with diversified and consensus VH/VL hypervariable sequences
US20070237764A1 (en) 2005-12-02 2007-10-11 Genentech, Inc. Binding polypeptides with restricted diversity sequences
US7846724B2 (en) * 2006-04-11 2010-12-07 Hoffmann-La Roche Inc. Method for selecting CHO cell for production of glycosylated antibodies
WO2007134050A2 (en) 2006-05-09 2007-11-22 Genentech, Inc. Binding polypeptides with optimized scaffolds
EP1975239A1 (en) * 2006-09-27 2008-10-01 Bundesrepublik Deutschland, letztvertreten durch den Präsidenten des Paul-Ehrlich-Instituts Prof. Dr. Johannes Löwer Pseudotyping of retroviral vectors, methods for production and use thereof for targeted gene transfer and high-throughput screening
US20080226635A1 (en) 2006-12-22 2008-09-18 Hans Koll Antibodies against insulin-like growth factor I receptor and uses thereof
CN100592373C (zh) 2007-05-25 2010-02-24 群康科技(深圳)有限公司 液晶显示面板驱动装置及其驱动方法
US8227577B2 (en) 2007-12-21 2012-07-24 Hoffman-La Roche Inc. Bivalent, bispecific antibodies
US9266967B2 (en) 2007-12-21 2016-02-23 Hoffmann-La Roche, Inc. Bivalent, bispecific antibodies
US20090162359A1 (en) 2007-12-21 2009-06-25 Christian Klein Bivalent, bispecific antibodies
US8242247B2 (en) 2007-12-21 2012-08-14 Hoffmann-La Roche Inc. Bivalent, bispecific antibodies
PT2235064E (pt) 2008-01-07 2016-03-01 Amgen Inc Método de preparação de moléculas heterodiméricas de fc de anticorpos utilizando efeitos de indução eletrostática
EP2098536A1 (en) * 2008-03-05 2009-09-09 4-Antibody AG Isolation and identification of antigen- or ligand-specific binding proteins
BRPI1014089A2 (pt) 2009-04-02 2016-04-19 Roche Glycart Ag anticorpos multiespecíficos que compreendem anticorpos de comprimento completo e fragmentos fab de cadeia simples
DK2417156T3 (en) 2009-04-07 2015-03-02 Roche Glycart Ag Trivalent, bispecific antibodies
RU2570633C2 (ru) 2009-05-27 2015-12-10 Ф.Хоффманн-Ля Рош Аг Три- или тетраспецифические антитела
US9676845B2 (en) 2009-06-16 2017-06-13 Hoffmann-La Roche, Inc. Bispecific antigen binding proteins
US8703132B2 (en) 2009-06-18 2014-04-22 Hoffmann-La Roche, Inc. Bispecific, tetravalent antigen binding proteins
EP2606064B1 (en) * 2010-08-16 2015-02-25 NovImmune S.A. Methods for the generation of multispecific and multivalent antibodies
CA2791109C (en) * 2011-09-26 2021-02-16 Merus B.V. Generation of binding molecules

Similar Documents

Publication Publication Date Title
JP2015503907A5 (enExample)
RU2014129736A (ru) Система индикации на основе полноразмерного антитела для эукариотических клеток и ее применение
EP3648587B1 (en) Cells, vertebrates, populations & methods
Burnett et al. Immunizations with diverse sarbecovirus receptor-binding domains elicit SARS-CoV-2 neutralizing antibodies against a conserved site of vulnerability
JP2014110814A5 (enExample)
JP6479024B2 (ja) 抗体親和性の最適化のための高スループットマウスモデル
Umotoy et al. Rapid and focused maturation of a VRC01-class HIV broadly neutralizing antibody lineage involves both binding and accommodation of the N276-glycan
Seo et al. Rapid generation of specific antibodies by enhanced homologous recombination
Fallet et al. Chronic viral infection promotes efficient germinal center B cell responses
Byrne et al. A tale of two specificities: bispecific antibodies for therapeutic and diagnostic applications
JP2014524243A5 (enExample)
HRP20210916T1 (hr) Miševi s ljudskim univerzalnim lakim lancem
Satofuka et al. Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci
JP2015502177A5 (enExample)
JP2012532598A5 (enExample)
JP2018138047A5 (enExample)
HRP20150262T1 (hr) Miševi sa adam6
JP2015519076A5 (enExample)
JP2015227386A5 (enExample)
JP2015525071A5 (enExample)
AU2018203713A1 (en) Histidine engineered light chain antibodies and genetically modified non-human animals for generating the same
JP2012521211A5 (enExample)
Drabek et al. Expression cloning and production of human heavy-chain-only antibodies from murine transgenic plasma cells
HRP20192218T1 (hr) Humanizirani miševi koji izražavaju teške lance koji sadrže vl domene
JP2011182801A5 (enExample)