MX2014007262A - Sistema de exhibicion de anticuerpos de longitud completa para celulas eucarioticas y su uso. - Google Patents

Sistema de exhibicion de anticuerpos de longitud completa para celulas eucarioticas y su uso.

Info

Publication number
MX2014007262A
MX2014007262A MX2014007262A MX2014007262A MX2014007262A MX 2014007262 A MX2014007262 A MX 2014007262A MX 2014007262 A MX2014007262 A MX 2014007262A MX 2014007262 A MX2014007262 A MX 2014007262A MX 2014007262 A MX2014007262 A MX 2014007262A
Authority
MX
Mexico
Prior art keywords
population
full length
eukaryotic cells
length antibody
membrane
Prior art date
Application number
MX2014007262A
Other languages
English (en)
Inventor
Hendrik Knoetgen
Peter Michael Huelsmann
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2014007262A publication Critical patent/MX2014007262A/es

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/26—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

En este documento se reporta un método de selección de una célula que expresa un anticuerpo biespecífico que comprende los pasos de (a) generar una población de células eucariotas mediante transducción con una población de partículas de virus lentiviral, por el que cada célula de la población de células exhibe un anticuerpo de longitud completa unido a la membrana que está codificado por el ácido nucleico lentiviral, y que se une específicamente a dos o más antígenos o dos o más epítopos en el mismo antígeno, y (b) seleccionar de la población de células eucariotas una célula que depende de las propiedades del anticuerpo de longitud completa unido a la membrana exhibida, por el que cada partícula de virus lentiviral de la población de partículas de virus lentiviral comprende un casete de expresión bicistrónico que comprende el IRES de EV71 para la expresión del anticuerpo unido a la membrana.
MX2014007262A 2011-12-22 2012-12-19 Sistema de exhibicion de anticuerpos de longitud completa para celulas eucarioticas y su uso. MX2014007262A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP11195375 2011-12-22
EP12179029 2012-08-02
PCT/EP2012/076163 WO2013092720A1 (en) 2011-12-22 2012-12-19 Full length antibody display system for eukaryotic cells and its use

Publications (1)

Publication Number Publication Date
MX2014007262A true MX2014007262A (es) 2014-08-01

Family

ID=47458959

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014007262A MX2014007262A (es) 2011-12-22 2012-12-19 Sistema de exhibicion de anticuerpos de longitud completa para celulas eucarioticas y su uso.

Country Status (12)

Country Link
US (1) US20150038354A1 (es)
EP (1) EP2794662A1 (es)
JP (1) JP2015503907A (es)
KR (1) KR20140107295A (es)
CN (1) CN104011080B (es)
BR (1) BR112014013035A2 (es)
CA (1) CA2854246A1 (es)
HK (1) HK1200849A1 (es)
MX (1) MX2014007262A (es)
RU (1) RU2625033C2 (es)
SG (1) SG11201403445YA (es)
WO (1) WO2013092720A1 (es)

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SG11201403445YA (en) 2014-07-30
CA2854246A1 (en) 2013-06-27
RU2014129736A (ru) 2016-02-20
BR112014013035A2 (pt) 2018-10-09
CN104011080A (zh) 2014-08-27
KR20140107295A (ko) 2014-09-04
HK1200849A1 (en) 2015-08-14
EP2794662A1 (en) 2014-10-29
CN104011080B (zh) 2017-10-20
RU2625033C2 (ru) 2017-07-11
JP2015503907A (ja) 2015-02-05
WO2013092720A1 (en) 2013-06-27
US20150038354A1 (en) 2015-02-05

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