MX2017005310A - Metodos basados en proximidad para seleccion de parejas para ligadura. - Google Patents

Metodos basados en proximidad para seleccion de parejas para ligadura.

Info

Publication number
MX2017005310A
MX2017005310A MX2017005310A MX2017005310A MX2017005310A MX 2017005310 A MX2017005310 A MX 2017005310A MX 2017005310 A MX2017005310 A MX 2017005310A MX 2017005310 A MX2017005310 A MX 2017005310A MX 2017005310 A MX2017005310 A MX 2017005310A
Authority
MX
Mexico
Prior art keywords
binding partners
selection
based methods
proximity based
target protein
Prior art date
Application number
MX2017005310A
Other languages
English (en)
Inventor
Xie Jia
A Lerner Richard
Zhang Hongkai
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Publication of MX2017005310A publication Critical patent/MX2017005310A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Esta invención provee métodos para identificar parejas para ligadura (v.gr., ligados de péptidos) que se liguen a una proteína objetivo (v.gr., un receptor celular). Los métodos implican la expresión co-localizada de la proteína objetivo y parejas de ligadura candidatas, y la selección de las parejas de ligadura basada en su proximidad en la membrana del plasma.
MX2017005310A 2014-10-20 2015-10-16 Metodos basados en proximidad para seleccion de parejas para ligadura. MX2017005310A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462066105P 2014-10-20 2014-10-20
PCT/US2015/055941 WO2016064673A1 (en) 2014-10-20 2015-10-16 Proximity based methods for selection of binding partners

Publications (1)

Publication Number Publication Date
MX2017005310A true MX2017005310A (es) 2018-03-01

Family

ID=55761351

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017005310A MX2017005310A (es) 2014-10-20 2015-10-16 Metodos basados en proximidad para seleccion de parejas para ligadura.

Country Status (11)

Country Link
US (1) US10655124B2 (es)
EP (1) EP3209626A4 (es)
JP (1) JP2017530721A (es)
CN (1) CN107207371A (es)
AU (1) AU2015336308B2 (es)
BR (1) BR112017008011A2 (es)
CA (1) CA2964895A1 (es)
EA (1) EA034306B1 (es)
IL (1) IL251771A0 (es)
MX (1) MX2017005310A (es)
WO (1) WO2016064673A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10201910999TA (en) * 2015-05-15 2020-01-30 Agency Science Tech & Res Native protein purification technology
WO2017214830A1 (zh) * 2016-06-14 2017-12-21 石庆学 特异促进 pd-1 基因高表达的慢病毒载体及其应用
WO2017214829A1 (zh) * 2016-06-14 2017-12-21 石庆学 特异促进 pd-l1 基因高表达的慢病毒表达载体及其应用
WO2019070161A2 (en) 2017-10-04 2019-04-11 Opko Pharmaceuticals, Llc ARTICLES AND METHODS FOR PERSONALIZED THERAPY OF CANCER

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10109855A1 (de) * 2001-03-01 2002-09-12 Stanislawski Thomas Polypeptide eines p53-Protein-spezifischen murinen alpha/beta T-Zell Rezeptors, diese kodierende Nukleinsäuren und deren Verwendung
DE10109854A1 (de) * 2001-03-01 2002-09-12 Thomas Stanislawski Polypeptide eines hdm2-Protein spezifischen murinen alpha/beta T-Zell Rezeptors, diese kodierende Nukleinsäuren und deren Verwendung
US6867005B2 (en) * 2001-10-24 2005-03-15 Beckman Coulter, Inc. Method and apparatus for increasing the dynamic range and accuracy of binding assays
DE10211063A1 (de) * 2002-03-13 2003-10-09 Axaron Bioscience Ag Neue Verfahren zur Detektion und Analyse von Protein-Interaktionen in vivo
EP2535346B1 (en) * 2005-08-31 2017-08-02 The Regents of The University of California Cellular libraries of peptide sequences (CLiPS) and methods of using the same
KR100794449B1 (ko) * 2007-03-29 2008-01-16 고려대학교 산학협력단 핵산 전달용 양이온성 인지질 나노입자 조성물
BRPI0811857A2 (pt) * 2007-05-14 2014-10-21 Biogen Idec Inc Regiões fc (scfc) de cadeia simples, polipeptídeos de aglutinação que as compreendem e métodos relacionados.
WO2008150496A2 (en) * 2007-05-31 2008-12-11 Genelux Corporation Assay for sensitivity to chemotherapeutic agents
WO2009009045A2 (en) * 2007-07-10 2009-01-15 The Scripps Research Institute Escape libraries of target polypeptides
EP2548030B1 (en) * 2010-03-18 2015-05-27 Cornell University Engineering correctly folded antibodies using inner membrane display of twin-arginine translocation intermediates
WO2013022739A1 (en) * 2011-08-05 2013-02-14 Leonard Joshua N Modular extracellular sensor architecture for cell-based biosensors
US20140308675A1 (en) * 2011-10-11 2014-10-16 Nissin Foods Holdings Co., Ltd. Mutagenicity test method using mammalian cells
CN104011080B (zh) * 2011-12-22 2017-10-20 弗·哈夫曼-拉罗切有限公司 用于真核细胞的全长抗体展示系统及其用途

Also Published As

Publication number Publication date
AU2015336308A1 (en) 2017-05-11
EP3209626A4 (en) 2018-03-21
US10655124B2 (en) 2020-05-19
IL251771A0 (en) 2017-06-29
BR112017008011A2 (pt) 2017-12-19
CN107207371A (zh) 2017-09-26
CA2964895A1 (en) 2016-04-28
WO2016064673A1 (en) 2016-04-28
AU2015336308B2 (en) 2020-05-14
EA201790725A1 (ru) 2018-01-31
EP3209626A1 (en) 2017-08-30
JP2017530721A (ja) 2017-10-19
US20180119133A1 (en) 2018-05-03
EA034306B1 (ru) 2020-01-27

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