JP2015503907A - 真核細胞のための全長抗体提示システムおよびその使用 - Google Patents

真核細胞のための全長抗体提示システムおよびその使用 Download PDF

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JP2015503907A
JP2015503907A JP2014547977A JP2014547977A JP2015503907A JP 2015503907 A JP2015503907 A JP 2015503907A JP 2014547977 A JP2014547977 A JP 2014547977A JP 2014547977 A JP2014547977 A JP 2014547977A JP 2015503907 A JP2015503907 A JP 2015503907A
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cells
cell
antibody
nucleic acid
antigen
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JP2015503907A5 (enExample
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ピーター ミカエル ヒュエルスマン
ピーター ミカエル ヒュエルスマン
ヘンドリク クノエトゲン
ヘンドリク クノエトゲン
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F Hoffmann La Roche AG
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/26—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
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  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2014547977A 2011-12-22 2012-12-19 真核細胞のための全長抗体提示システムおよびその使用 Ceased JP2015503907A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP11195375 2011-12-22
EP11195375.8 2011-12-22
EP12179029.9 2012-08-02
EP12179029 2012-08-02
PCT/EP2012/076163 WO2013092720A1 (en) 2011-12-22 2012-12-19 Full length antibody display system for eukaryotic cells and its use

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JP2015503907A true JP2015503907A (ja) 2015-02-05
JP2015503907A5 JP2015503907A5 (enExample) 2016-02-12

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US (1) US20150038354A1 (enExample)
EP (1) EP2794662A1 (enExample)
JP (1) JP2015503907A (enExample)
KR (1) KR20140107295A (enExample)
CN (1) CN104011080B (enExample)
BR (1) BR112014013035A2 (enExample)
CA (1) CA2854246A1 (enExample)
HK (1) HK1200849A1 (enExample)
MX (1) MX2014007262A (enExample)
RU (1) RU2625033C2 (enExample)
SG (1) SG11201403445YA (enExample)
WO (1) WO2013092720A1 (enExample)

Cited By (7)

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JP2019509063A (ja) * 2016-03-19 2019-04-04 エフ１ オンコロジー，インコーポレイティド リンパ球に形質導入を行うための方法及び組成物、並びにその制御された増加
JP2023093642A (ja) * 2016-10-14 2023-07-04 デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド モジュラー四価二重特異性抗体プラットフォーム
JP2024509251A (ja) * 2021-03-08 2024-02-29 ナンジン、ジェンスクリプト、バイオテック、カンパニー、リミテッド デュアルウイルスベクター系による抗体送達
KR20250022761A (ko) 2022-06-10 2025-02-17 후지필름 다이오신쓰 바이오테크놀로지스 유케 리미티드 세포의 작출 방법, 헤테로 다량체 단백질의 제조 방법, 바이스페시픽 항체의 제조 방법, 벡터 세트, 포유동물 세포, cho 세포, 및 세포 풀의 작출 방법
US12258574B2 (en) 2016-03-19 2025-03-25 Exuma Biotech Corp. Methods and compositions for transducing lymphocytes and regulating the activity thereof
US12325728B2 (en) 2016-03-19 2025-06-10 Exuma Biotech Corp. Methods and compositions for genetically modifying lymphocytes to express polypeptides comprising the intracellular domain of CD79A and CD79B
US12590321B2 (en) 2016-03-19 2026-03-31 Exuma Biotech Corp. Methods and compositions for genetically modifying and expanding lymphocytes and regulating the activity thereof

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US10662256B2 (en) 2010-07-26 2020-05-26 Trianni, Inc. Transgenic mammals and methods of use thereof
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CN104818295A (zh) * 2015-02-03 2015-08-05 武汉友芝友生物制药有限公司 制备和筛选表达双特异性抗体细胞株的方法
EP3384030A4 (en) 2015-12-03 2019-07-03 Trianni, Inc. IMPROVED IMMUNOGLULINIVITY
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BR112018071283A2 (pt) 2016-04-20 2019-02-12 Regeneron Pharma célula, conjunto de vetores para expressar uma proteína de ligação a antígeno biespecífica em uma célula, conjunto de vetores, método, e, método para produção de uma proteína de ligação a antígeno.
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CN110872356B (zh) * 2018-09-03 2023-06-13 广西慧宝源健康产业有限公司 双特异性抗体及其使用方法
CN113544275A (zh) * 2019-03-05 2021-10-22 信达生物制药(苏州)有限公司 展示与分泌目的多肽的酵母展示系统及其用途
CN114829407B (zh) * 2019-09-23 2024-06-21 南开大学 利用哺乳动物展示筛选FcγR特异性结合Fc
MX2022012005A (es) * 2020-03-27 2022-10-21 Ddbio Co Ltd Shang Hai Metodo para el despliegue de anticuerpo biespecifico en la superficie de celula de mamifero y vector.
CN113150122B (zh) * 2021-05-19 2023-03-17 上海儒克生物科技有限公司 高通量全兔源单克隆抗体的制备方法
EP4453019A1 (en) * 2021-12-23 2024-10-30 Helsingin Yliopisto Method for screening a signal peptide for efficient expression and secretion of a heterologous polypeptide in mammalian cells
CN119574886A (zh) * 2024-11-29 2025-03-07 苏州泓迅生物科技股份有限公司 一种基于动物细胞表面展示库的抗体筛选方法及应用

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WO2013092720A1 (en) 2013-06-27
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