FR2504148B1 - - Google Patents

Info

Publication number
FR2504148B1
FR2504148B1 FR8206786A FR8206786A FR2504148B1 FR 2504148 B1 FR2504148 B1 FR 2504148B1 FR 8206786 A FR8206786 A FR 8206786A FR 8206786 A FR8206786 A FR 8206786A FR 2504148 B1 FR2504148 B1 FR 2504148B1
Authority
FR
France
Prior art keywords
novel
debranching enzyme
glucoamylase
thermostability
cultivating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
FR8206786A
Other languages
English (en)
French (fr)
Other versions
FR2504148A1 (fr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Industri AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22970517&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=FR2504148(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novo Industri AS filed Critical Novo Industri AS
Publication of FR2504148A1 publication Critical patent/FR2504148A1/fr
Application granted granted Critical
Publication of FR2504148B1 publication Critical patent/FR2504148B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Fodder In General (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Detergent Compositions (AREA)
  • Jellies, Jams, And Syrups (AREA)
FR8206786A 1981-04-20 1982-04-20 Substance enzymatique deramifiante, procede pour sa preparation et utilisation de celle-ci Granted FR2504148A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US25595281A 1981-04-20 1981-04-20

Publications (2)

Publication Number Publication Date
FR2504148A1 FR2504148A1 (fr) 1982-10-22
FR2504148B1 true FR2504148B1 (en:Method) 1984-05-18

Family

ID=22970517

Family Applications (1)

Application Number Title Priority Date Filing Date
FR8206786A Granted FR2504148A1 (fr) 1981-04-20 1982-04-20 Substance enzymatique deramifiante, procede pour sa preparation et utilisation de celle-ci

Country Status (18)

Country Link
EP (1) EP0063909B2 (en:Method)
JP (2) JPS57174089A (en:Method)
KR (1) KR890002413B1 (en:Method)
AT (1) ATE28216T1 (en:Method)
AU (1) AU548122B2 (en:Method)
BE (1) BE892904A (en:Method)
CA (1) CA1199292A (en:Method)
CH (1) CH659825A5 (en:Method)
DE (1) DE3276707D1 (en:Method)
DK (1) DK153569C (en:Method)
ES (2) ES511504A0 (en:Method)
FI (1) FI79345C (en:Method)
FR (1) FR2504148A1 (en:Method)
GB (1) GB2097405B (en:Method)
IE (1) IE53031B1 (en:Method)
IN (1) IN155660B (en:Method)
IT (1) IT1190780B (en:Method)
MX (1) MX174149B (en:Method)

Families Citing this family (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58183092A (ja) * 1982-04-19 1983-10-26 ノボ・インダストリ・アクテイ−ゼルスカブ 枝切り酵素
CA1211314A (en) * 1983-05-20 1986-09-16 William F. Line Production of dextrose and maltose syrups using an enzyme derived from rice
IE58094B1 (en) * 1983-09-11 1993-06-30 Gist Brocades Nv Novel enzyme product and its use in the saccharification of starch
JPS60186283A (ja) * 1984-03-07 1985-09-21 Agency Of Ind Science & Technol 微生物による新規な耐熱性プルラナ−ゼａの製造法
US4673633A (en) * 1984-06-28 1987-06-16 Cooperbiomedical, Inc. Method of determining cystic fibrosis ciliostatic factor
US4628028A (en) * 1985-05-23 1986-12-09 Cpc International Inc. Novel thermostable pullulanase enzyme and method for its production
GB8609288D0 (en) * 1986-04-16 1986-05-21 Ici Plc Debranching enzyme
US5234823A (en) * 1986-06-30 1993-08-10 Novo Industri A/S Chimeric enzymes
US5055403A (en) * 1989-06-26 1991-10-08 Enzyme Bio-Systems, Ltd. Thermoduric and aciduric pullulanase enzyme and method for its production
EP0405283A3 (en) * 1989-06-26 1991-03-06 Enzyme Bio-Systems Ltd. Novel thermoduric and aciduric pullulanase enzyme and method for its production
DK47291D0 (da) * 1991-03-15 1991-03-15 Novo Nordisk As Pullulanase
JPH05236959A (ja) * 1992-02-28 1993-09-17 Yoshiyuki Takasaki プルラナーゼ、その製造法及び該酵素を用いる澱粉の 糖化法
FR2688800B1 (fr) * 1992-03-19 1995-06-16 Roquette Freres Procede de preparation de polysaccharides peu digestibles, eventuellement hydrogenes.
BE1006483A3 (fr) * 1992-12-28 1994-09-13 Solvay Pullulanase, microorganismes la produisant, procedes de preparation de cette pullulanase, utilisations et compositions comprenant celle-ci.
EP0605040B1 (fr) * 1992-12-28 1999-08-11 Genencor International, Inc. Pullulanase, microorganismes la produisant, procédés de préparation de cette pullulanase et utilisations de celle-ci
ES2250969T3 (es) 1994-03-29 2006-04-16 Novozymes A/S Amilasa alcalina de bacilo.
AR000862A1 (es) 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
EP0904360B1 (en) 1996-04-30 2013-07-31 Novozymes A/S alpha-AMYLASE MUTANTS
EP2206768B1 (en) 1997-10-13 2015-04-01 Novozymes A/S Alpha-amylase mutants
WO1999023211A1 (en) 1997-10-30 1999-05-14 Novo Nordisk A/S α-AMYLASE MUTANTS
AU757935B2 (en) 1998-02-27 2003-03-13 Novozymes A/S Maltogenic alpha-amylase variants
WO2000060059A2 (en) 1999-03-30 2000-10-12 NovozymesA/S Alpha-amylase variants
CN100523181C (zh) 1999-03-31 2009-08-05 诺维信公司 具有碱性α-淀粉酶活性的多肽及其编码核酸
EP2011864B1 (en) 1999-03-31 2014-12-31 Novozymes A/S Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same
EP1263942B1 (en) 2000-03-08 2013-11-06 Novozymes A/S Variants with altered properties
JP4855632B2 (ja) 2000-08-01 2012-01-18 ノボザイムス アクティーゼルスカブ 変更された性質を有するα−アミラーゼ突然変異体
US20040091994A1 (en) 2000-10-13 2004-05-13 Carsten Andersen Alpha-amylase variant with altered properties
AU2002254876A1 (en) 2001-05-15 2002-11-25 Novozymes A/S Alpha-amylase variant with altered properties
AU2004252572B2 (en) 2003-06-25 2011-09-08 Novozymes A/S Polypeptides having alpha-amylase activity and polypeptides encoding same
BRPI0512776A (pt) 2004-07-05 2008-04-08 Novozymes As variante de uma alfa-amilase tipo termamil originária, construto de dna, vetor de expressão recombinante, célula, composição, aditivo de detergente, composição detergente, composição de lavagem de roupa manual ou automática, uso de uma variante de alfa-amilase ou composição, e, método de produzir uma variante
US20080032024A1 (en) 2004-08-02 2008-02-07 Lars Beier Maltogenic Alpha-Amylase Variants
US20080280328A1 (en) 2005-11-18 2008-11-13 Novozymes A/S Glucoamylase Variants
JP5520828B2 (ja) 2007-11-05 2014-06-11 ダニスコ・ユーエス・インク 改変される特徴を有するバシルス種（Ｂａｃｉｌｌｕｓｓｐ．）ＴＳ‐２３アルファ‐アミラーゼ変異体
AR069168A1 (es) 2007-11-05 2010-01-06 Danisco Us Inc Genencor Div Variantes de alfa -amilasas con propiedades alteradas
JP2011510681A (ja) 2008-02-04 2011-04-07 ダニスコ・ユーエス・インク 改変された特性をもつｔｓ２３アルファ‐アミラーゼ変異体
JP5492879B2 (ja) 2008-06-06 2014-05-14 ダニスコ・ユーエス・インク 改善された性質を有するゲオバチルスステアロセレモフィリス（Ｇｅｏｂａｃｉｌｌｕｓｓｔｅａｒｏｔｈｅｒｍｏｐｈｉｌｕｓ）アルファアミラーゼ（ＡｍｙＳ）変異体
EP2337837B2 (en) 2008-09-25 2016-10-26 Danisco US Inc. Alpha-amylase blends and methods for using said blends
EP2414515A2 (en) 2009-04-01 2012-02-08 Danisco US Inc. Cleaning system comprising an alpha-amylase and a protease
US8545907B2 (en) 2009-08-07 2013-10-01 Danisco Us Inc. Alpha-amylase blend for starch processing and method of use thereof
CN102933227A (zh) 2009-12-22 2013-02-13 诺维信公司 包含增强性多肽和淀粉降解酶的组合物及其用途
EP2521773A1 (en) 2010-01-04 2012-11-14 Novozymes A/S Alpha-amylases
EP2534236B1 (en) 2010-02-10 2018-05-30 Novozymes A/S Variants and compositions comprising variants with high stability in presence of a chelating agent
EP2357220A1 (en) 2010-02-10 2011-08-17 The Procter & Gamble Company Cleaning composition comprising amylase variants with high stability in the presence of a chelating agent
KR20140056237A (ko) 2011-06-30 2014-05-09 노보자임스 에이/에스 알파-아밀라제 변이체
JP2014528241A (ja) 2011-09-29 2014-10-27 ダニスコ・ユーエス・インク 高濃度デンプン粒の液化及び糖化
CA3253390A1 (en) 2011-10-17 2025-06-17 Novozymes A/S Variants of alpha-amylase and the polynucleotides encoding them
KR102046075B1 (ko) 2011-10-17 2019-11-18 노보자임스 에이/에스 알파-아밀라아제 변이체 및 이를 암호화하는 폴리뉴클레오티드
WO2013148152A1 (en) 2012-03-28 2013-10-03 Danisco Us Inc. Method for making high maltose syrup
CA2878988A1 (en) 2012-08-16 2014-02-20 Danisco Us Inc. Method of using alpha-amylase from aspergillus clavatus and pullulanase for saccharification
CA2893270C (en) 2012-12-11 2024-01-02 Danisco Us Inc. Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof
CN104903461A (zh) 2012-12-20 2015-09-09 丹尼斯科美国公司 将支链淀粉酶和来自土曲霉的α-淀粉酶用于糖化的方法
EP3022300B1 (en) 2013-07-17 2018-07-11 Novozymes A/S Pullulanase chimeras and polynucleotides encoding same
DK3097192T3 (en) 2014-01-22 2018-11-19 Novozymes As PULLULANASE VARIATIONS AND POLYNUCLEOTIDES CODING THEM
WO2015143144A1 (en) 2014-03-19 2015-09-24 Novozymes A/S Method for enhancing activity of an x143 polypeptide
WO2015157656A1 (en) 2014-04-10 2015-10-15 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
WO2016062875A2 (en) 2014-10-23 2016-04-28 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
WO2016087327A1 (en) 2014-12-01 2016-06-09 Novozymes A/S Polypeptides having pullulanase activity comprising the x25, x45 and cbm41 domains
CN108368528A (zh) 2015-10-14 2018-08-03 诺维信公司 葡糖淀粉酶变体和编码它们的多核苷酸
MX2018007485A (es) 2015-12-30 2018-08-01 Novozymes As Variantes de enzimas y polinucleotidos que las codifican.
WO2017191160A1 (en) 2016-05-03 2017-11-09 Novozymes A/S Alpha-amylase variants and polynucleotides encoding the same
US11186833B2 (en) 2016-05-09 2021-11-30 Novozymes A/S Variant polypeptides with improved performance and use of the same
DK3478831T3 (da) 2016-06-30 2021-04-19 Danisco Us Inc Asparaginproteaser
EP3272767B1 (en) 2016-07-21 2020-11-25 Fornia BioSolutions, Inc. G24 glucoamylase compositions and methods
DK3487996T5 (da) 2016-07-21 2024-09-02 Novozymes As Serinproteasevarianter og polynukleotider, der koder for samme
WO2018017105A1 (en) 2016-07-21 2018-01-25 Fornia Biosolutions, Inc. G24 glucoamylase compositions and methods
US11891645B2 (en) 2016-07-21 2024-02-06 Novozymes A/S Serine protease variants and polynucleotides encoding same
US9598680B1 (en) 2016-08-05 2017-03-21 Fornia Biosolutions, Inc. G16 glucoamylase compositions and methods
EP3548615A1 (en) 2016-11-29 2019-10-09 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
US20190330577A1 (en) 2016-12-21 2019-10-31 Dupont Nutrition Biosciences Aps Methods of using thermostable serine proteases
US20200015499A1 (en) 2017-03-15 2020-01-16 Dupont Nutrition Biosciences Aps Trypsin-like serine proteases and uses thereof
RU2019132448A (ru) 2017-03-15 2021-04-15 ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС Способы применения сериновой протеазы архей
US11279921B2 (en) 2017-04-11 2022-03-22 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
US20200181663A1 (en) 2017-08-30 2020-06-11 Novozymes A/S Combined use of at least one endoprotease and at least one exo-protease in an ssf process for improving ethanol yield
ES2955269T3 (es) 2017-12-08 2023-11-29 Novozymes As Variantes de alfa-amilasa y polinucleótidos que codifican las mismas
BR112020011173A2 (pt) 2017-12-08 2020-11-17 Novozymes A/S variante de alfa-amilase, polinucleotídeo, composição, construto de ácido nucleico, vetor de expressão, célula hospedeira, método de produção de uma variante de alfa-amilase, uso da variante, e, processo para produção de um xarope a partir de material contendo amido.
US11377648B2 (en) 2018-04-09 2022-07-05 Novozymes A/S Polypeptides having alpha-amylase activity and polynucleotides encoding same
US11535836B2 (en) 2018-12-21 2022-12-27 Fornia Biosolutions, Inc. Variant G6P G7P glucoamylase compositions and methods
WO2020188095A1 (en) 2019-03-21 2020-09-24 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
US20230142991A1 (en) 2020-02-10 2023-05-11 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same

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Also Published As

Publication number Publication date
IE820907L (en) 1982-10-20
ES520561A0 (es) 1984-11-01
JPS6143994A (ja) 1986-03-03
FR2504148A1 (fr) 1982-10-22
AU548122B2 (en) 1985-11-21
FI821355L (fi) 1982-10-21
ES8307291A1 (es) 1983-06-16
DK153569C (da) 1988-12-05
KR830010192A (ko) 1983-12-26
DK153569B (da) 1988-07-25
IN155660B (en:Method) 1985-02-16
DK172882A (da) 1982-12-29
IT8220806A0 (it) 1982-04-19
FI821355A0 (fi) 1982-04-19
CA1199292A (en) 1986-01-14
FI79345C (fi) 1989-12-11
ATE28216T1 (de) 1987-07-15
FI79345B (fi) 1989-08-31
AU8280882A (en) 1982-10-28
DE3276707D1 (en) 1987-08-13
ES8500994A1 (es) 1984-11-01
MX174149B (es) 1994-04-25
JPS57174089A (en) 1982-10-26
EP0063909B1 (en) 1987-07-08
EP0063909A1 (en) 1982-11-03
IE53031B1 (en) 1988-05-11
EP0063909B2 (en) 1990-09-12
IT1190780B (it) 1988-02-24
CH659825A5 (de) 1987-02-27
GB2097405B (en) 1984-08-15
GB2097405A (en) 1982-11-03
BE892904A (fr) 1982-10-19
JPS6225036B2 (en:Method) 1987-06-01
ES511504A0 (es) 1983-06-16
KR890002413B1 (ko) 1989-07-03

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