KR830010192A - 디브랜칭 효소 생성물, 그 제법 및 그 이용 방법 - Google Patents

디브랜칭 효소 생성물, 그 제법 및 그 이용 방법 Download PDF

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KR830010192A
KR830010192A KR1019820001728A KR820001728A KR830010192A KR 830010192 A KR830010192 A KR 830010192A KR 1019820001728 A KR1019820001728 A KR 1019820001728A KR 820001728 A KR820001728 A KR 820001728A KR 830010192 A KR830010192 A KR 830010192A
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debranching enzyme
ncib
bacillus
strain
enzyme product
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카밀라 닐젠 그레테
디어스 이반
오트렆 헬레
에디문트 노만 바리
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케이 할리스 뫼랠
노보 인두스트리 아크티에 셀스카브
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)

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Abstract

내용 없음

Description

디브랜칭 효소 생성물, 그 제법 및 그 이용 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
도면은 여러온도 및 pH에서의 정제된 풀루라나제의 활성을 표시하는 그래프

Claims (16)

  1. 다음의 특성을 갖는 플루라나제 타입의 신규의 디브랜칭 효소를 함유하는 디브랜칭효소 생성물 :
    a) 그것은 적당한 영양배지속에서, 이하에 바실루스 아시도 풀루리티쿠스(Bacillus Acidopullulyticus)라고 명명된 분류군에 속하는 균주를 배양하므로서 생성되는 발효즙으로 부터 얻어지며,
    b) 그것은 바실루스균주 NCIB 11607로 부터 유도된 디 브랜칭효소와 기본적으로 동일한 효소화학적 특성을 갖고 있으며, 면역학적으로도 동일하거나 부분적으로 동일한 특성을 갖고 이으며,
    c) 그것의 최적활성은, 아세트산염완충액(0.05M)속에서 pH 4-5로, 30분간 배양시켜서 측정했을때, 적어도 60℃정도에서 나타나며,
    d) 그것의 최적 pH는, 아세트산염완충액(0.05M)속에서, 60℃에서 30분간 배양시켜서 구한바에 의하면 3.5내지 5.5범위이내이며,
    e) 그것은 pH 5에서 포도당용액(중등으로 30퍼센트의 건조고체)속에서 측정했을 때 60℃에서 72시간후에도 적어도 50%의 잔류활성을 갖고 있음.
  2. 특허청구의 범위 1에 있어서의 디브랜칭효소 생성물로서 디브랜칭효소의 활성범위가 그람당 10내지 350,000풀루라나제 단위 이내인 것을 특징으로 하는 디브랜칭 효소 생성물.
  3. 특허청구 범위 2의 디브랜칭효소생성문로서 그 활성범위가 그람당 100내지 15,000풀루라나제 단위 이내의 것을 특징으로 하는 디브랜칭 효소 생성물.
  4. 디브랜칭효소를 생성시키는 바실루스 아시도풀루리티쿠스에 속하는 균주를 적당한 영양배지속에서 배양하므로서 만들어지는 디브랜칭 효소생성물과 그 디 브랜칭효소를 영양배지로 부터 회수하는 방법.
  5. 특허청구 범위 1의 디 브랜칭효소 생성물을 제조하는 방법에 있어, 탄소와 질소원 및 무기염을 함유하는 적당한 영양배지 속에서, 바실루스아시도풀루리티쿠스 분류군에 속하고 디브랜 칭효소를 생성시키는간균주를 배양하고 이어서 그 디브랜칭 효소생성물을 회수하는 것으로 되어 있는 것을 특징으로 하는 디브랜칭효소생성물의 제조방법.
  6. 특허청구 범위 제5항에 있어서, NCIB 11607, NCIB 11610 및 이들의 변종과 돌연변이체로 이루어진 군으로 부터 바실루스 아시도풀루리티쿠스가 선택되는 것을 특징으로 하는 제조방법.
  7. 특허청구범위 제6항에 있어서, NCIB 11638과 NCIB 11647로 구성된 돌연변위체군으로 부터 균주가 선택되는 것을 특징으로 하는 제조방법.
  8. 특허청구범위 제5항에 있어서, 바실루스아시도풀루리티쿠스균주는 NCIB 11611 또는 그 변종이나 돌연변위체인 것을 특징으로 하는 제조방법.
  9. 특허청구범위 제5항에 있어서, 바실루스아시도풀루리티쿠스균주는 NCIB 11636 또는 그 변종이나 돌연변이체인 것을 특징으로 하는 제조방법.
  10. 특허청구범위 제5항에 있어서, 바실루스 아시도 풀루리티쿠스균주는 NCIB 11637 또는 그 변종이나 돌연변이체인 것을 특징으로 하는 제조방법.
  11. 특허청구범위 제5항에 있어서, 바실루스 아시도 풀루리티쿠스균주는 NCIB 11639 또는 그 변종이나 돌연변이체인 것을 특징으로 하는 제조방법.
  12. 특허청구범위 제4항의 신규의 디브랜칭효소의 유효량과, 글루코아밀라아제와 베타-아밀라아제로 구성되는 균으로 부터 선택된 당화효소로된 효소계의 존재하에서, 녹말이나 녹말가루 분해물을 당화시키는 것을 특징으로 하는 녹말에서 텍스트로오스 및 또는 말토오스를 함유하는 시럽을 제조하는 방법.
  13. 특허청구범위 제12항에 있어서, 최저 30중량 퍼센트의 건조고체를 함유하는 녹말가수분해물을 당화시키는 과정을 더욱 포함하는 제조방법.
  14. 특허청구범위 제12항에 있어서, pH 범위 3.5내지 5.5와 온도 55℃내지 65℃이내에서 당화가 행해지는 것을 특징으로하는 제조방법.
  15. 특허청구범위 제12항에 있어서, 글루코아밀라아제와 베타-아밀라아제의 투입량은 건조고체 1그람당 각기 0.05내지 0.5 AG 단위와 0.005내지 0.3베타-아밀라아제 단위이내인 것을 특징으로 하는 제조방법.
  16. 특허청구범위 제15항에 있어서, 디브랜칭효소의 투입량은, 건조고체 1그람당 0.005내지 풀루라나제 단위 이내인 것을 특징으로 하는 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR8201726A 1981-04-20 1982-04-19 디브랜칭 효소 생성물, 그 제법 및 그 이용 방법 KR890002413B1 (ko)

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KR890002413B1 KR890002413B1 (ko) 1989-07-03

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IT8220806A0 (it) 1982-04-19
EP0063909B1 (en) 1987-07-08
FI79345C (fi) 1989-12-11
MX174149B (es) 1994-04-25
GB2097405A (en) 1982-11-03
AU8280882A (en) 1982-10-28
BE892904A (fr) 1982-10-19
KR890002413B1 (ko) 1989-07-03
EP0063909A1 (en) 1982-11-03
IT1190780B (it) 1988-02-24
DK153569B (da) 1988-07-25
ES520561A0 (es) 1984-11-01
FI821355A0 (fi) 1982-04-19
IE820907L (en) 1982-10-20
ES8500994A1 (es) 1984-11-01
EP0063909B2 (en) 1990-09-12
DK153569C (da) 1988-12-05
DE3276707D1 (en) 1987-08-13
CH659825A5 (de) 1987-02-27
JPS6225036B2 (ko) 1987-06-01
ES511504A0 (es) 1983-06-16
ATE28216T1 (de) 1987-07-15
JPS6143994A (ja) 1986-03-03
FR2504148B1 (ko) 1984-05-18
DK172882A (da) 1982-12-29
FI79345B (fi) 1989-08-31
ES8307291A1 (es) 1983-06-16
FR2504148A1 (fr) 1982-10-22
AU548122B2 (en) 1985-11-21
FI821355L (fi) 1982-10-21
CA1199292A (en) 1986-01-14
JPS57174089A (en) 1982-10-26
IN155660B (ko) 1985-02-16
GB2097405B (en) 1984-08-15
IE53031B1 (en) 1988-05-11

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