DK0605040T3 - Pullulanase, mikroorganismer producerende denne fremgangsmåde il fremstilling af denne pullulanase og anvendelse deraf - Google Patents

Pullulanase, mikroorganismer producerende denne fremgangsmåde il fremstilling af denne pullulanase og anvendelse deraf

Info

Publication number
DK0605040T3
DK0605040T3 DK93203593T DK93203593T DK0605040T3 DK 0605040 T3 DK0605040 T3 DK 0605040T3 DK 93203593 T DK93203593 T DK 93203593T DK 93203593 T DK93203593 T DK 93203593T DK 0605040 T3 DK0605040 T3 DK 0605040T3
Authority
DK
Denmark
Prior art keywords
pullulanase
relates
preparation
dna molecule
vector containing
Prior art date
Application number
DK93203593T
Other languages
English (en)
Inventor
Philippe Deweer
Antoine Amory
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from BE9201156A external-priority patent/BE1006483A3/fr
Priority claimed from BE9300744A external-priority patent/BE1007313A3/fr
Priority claimed from BE9301278A external-priority patent/BE1007723A6/fr
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of DK0605040T3 publication Critical patent/DK0605040T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Mechanical Means For Catching Fish (AREA)
  • Selective Calling Equipment (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Saccharide Compounds (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
DK93203593T 1992-12-28 1993-12-20 Pullulanase, mikroorganismer producerende denne fremgangsmåde il fremstilling af denne pullulanase og anvendelse deraf DK0605040T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
BE9201156A BE1006483A3 (fr) 1992-12-28 1992-12-28 Pullulanase, microorganismes la produisant, procedes de preparation de cette pullulanase, utilisations et compositions comprenant celle-ci.
BE9300744A BE1007313A3 (fr) 1993-07-15 1993-07-15 Pullulanase, microorganismes la produisant, procedes de preparation de cette pullulanase et utilisations de celle-ci.
BE9301278A BE1007723A6 (fr) 1993-11-19 1993-11-19 Pullulanase, microorganismes la produisant, procedes de preparation de cette pullulanase et utilisations de celle-ci.

Publications (1)

Publication Number Publication Date
DK0605040T3 true DK0605040T3 (da) 2000-03-13

Family

ID=27159793

Family Applications (1)

Application Number Title Priority Date Filing Date
DK93203593T DK0605040T3 (da) 1992-12-28 1993-12-20 Pullulanase, mikroorganismer producerende denne fremgangsmåde il fremstilling af denne pullulanase og anvendelse deraf

Country Status (13)

Country Link
US (6) US5817498A (da)
EP (1) EP0605040B1 (da)
JP (1) JPH06217770A (da)
KR (1) KR100319442B1 (da)
CN (1) CN1061089C (da)
AT (1) ATE183236T1 (da)
AU (2) AU686574B2 (da)
CA (1) CA2112028C (da)
DE (1) DE69325981T2 (da)
DK (1) DK0605040T3 (da)
ES (1) ES2137222T3 (da)
FI (1) FI116531B (da)
MX (1) MX9400123A (da)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0605040T3 (da) * 1992-12-28 2000-03-13 Genencor Int Pullulanase, mikroorganismer producerende denne fremgangsmåde il fremstilling af denne pullulanase og anvendelse deraf
EP0850307B1 (en) * 1995-09-13 2005-11-09 Genencor International, Inc. Alkaliphilic and thermophilic microorganisms and enzymes obtained therefrom
US6962263B2 (en) 1996-01-24 2005-11-08 Sambrailo Packaging, Inc. Produce packaging system having produce containers with double-arched ventilation channels
DE19627932A1 (de) * 1996-07-11 1998-01-15 Boehringer Mannheim Gmbh Sensitiver Epstein-Barr-Virus DNA-Nachweis
WO1998024799A1 (en) 1996-12-06 1998-06-11 Diversa Corporation Glycosidase enzymes
US7279316B2 (en) * 1996-12-06 2007-10-09 Verenium Corporation Enzymes having glycosidase activity and methods of use thereof
US7449320B2 (en) 1998-03-04 2008-11-11 Genencor International, Inc. Truncated forms of pullulanase
US6639126B1 (en) 1999-12-06 2003-10-28 Pioneer Hi-Bred International, Inc. Production of modified polysaccharides
US6350599B1 (en) 2000-01-12 2002-02-26 Novozymes A/S Pullulanase variants and methods for preparing such variants with predetermined properties
US7081261B2 (en) * 2002-05-14 2006-07-25 National Starch And Chemical Investment Holding Corporation Resistant starch prepared by isoamylase debranching of low amylose starch
US20050233030A1 (en) 2004-03-10 2005-10-20 Broin And Associates, Inc. Methods and systems for producing ethanol using raw starch and fractionation
CN102210376B (zh) * 2003-03-10 2014-12-31 波伊特研究股份有限公司 利用生淀粉生产乙醇的方法
US7700327B2 (en) * 2003-09-08 2010-04-20 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Resistant starch with cooking properties similar to untreated starch
US20050239181A1 (en) * 2004-03-10 2005-10-27 Broin And Associates, Inc. Continuous process for producing ethanol using raw starch
WO2006119386A2 (en) * 2005-05-02 2006-11-09 Broin And Associates, Inc. Methods and systems for producing ethanol using raw starch and fractionation
CN101268195A (zh) 2005-09-20 2008-09-17 诺维信北美公司 产生发酵产物的方法
US7919289B2 (en) 2005-10-10 2011-04-05 Poet Research, Inc. Methods and systems for producing ethanol using raw starch and selecting plant material
WO2007144393A1 (en) * 2006-06-15 2007-12-21 Novozymes, Inc. Mashing process
US8765199B2 (en) 2006-06-15 2014-07-01 Novozymes A/S Mashing process
US9969996B2 (en) 2006-08-04 2018-05-15 Amano Enzyme Inc. Method for making mutated pullulanase enzyme, mutated pullulanase enzyme, and microorganism expressing the same
US20090280553A1 (en) * 2006-08-04 2009-11-12 Amano Enzyme Inc. Method for designing mutated enzyme, method for preparing the same, and mutated enzyme
US7968691B2 (en) * 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity
WO2009074650A2 (en) 2007-12-12 2009-06-18 Novozymes A/S Brewing process
CN105623937A (zh) * 2007-12-12 2016-06-01 诺维信公司 糖化方法
WO2010043538A2 (en) 2008-10-15 2010-04-22 Novozymes A/S Brewing process
US9068206B1 (en) 2009-03-03 2015-06-30 Poet Research, Inc. System for treatment of biomass to facilitate the production of ethanol
US20100233771A1 (en) * 2009-03-03 2010-09-16 Mcdonald William F System for pre-treatment of biomass for the production of ethanol
US8450094B1 (en) 2009-03-03 2013-05-28 Poet Research, Inc. System for management of yeast to facilitate the production of ethanol
MX2011009269A (es) * 2009-03-03 2011-09-26 Poet Res Inc Fermentacion de biomasa para la produccion de etanol.
EP2430176B1 (en) * 2009-05-11 2024-08-07 Danisco US Inc. Improved production of maltotetraose syrup using a pseudomonas saccharophila maltotetraohydrolase variant and a debranching enzyme
CN102647918B (zh) 2009-08-07 2015-05-27 丹尼斯科美国公司 用于淀粉加工的α-淀粉酶混合物及其使用方法
BR112012011106C8 (pt) 2009-11-13 2019-11-26 Novozymes As método de mosturação
US10800999B2 (en) 2011-04-15 2020-10-13 Novozymes A/S Method for production of brewers wort
US20150037844A1 (en) 2011-09-29 2015-02-05 Danisco Us Inc. Liquefaction and saccharification of granular starch at high concentration
CN113930458A (zh) 2011-10-11 2022-01-14 诺维信北美公司 用于产生发酵产物的方法
WO2013148152A1 (en) 2012-03-28 2013-10-03 Danisco Us Inc. Method for making high maltose syrup
CN104271729A (zh) 2012-05-11 2015-01-07 诺维信公司 一种酿造方法
WO2014028434A2 (en) 2012-08-16 2014-02-20 Danisco Us Inc. Method of using alpha-amylase from aspergillus clavatus and pullulanase for saccharification
WO2014060474A1 (en) 2012-10-17 2014-04-24 Novozymes A/S Method for production of brewers wort
EP2931872B1 (en) 2012-12-11 2018-01-17 Danisco US Inc. Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof
US20160010128A1 (en) 2012-12-20 2016-01-14 Danisco Us Inc. Method of using alpha-amylase from aspergillus terreus and pullulanase for saccharification
JP6502314B2 (ja) * 2013-03-15 2019-04-17 ビーエーエスエフ エンザイムズ エルエルシー プルラナーゼ活性を有する酵素
EP3022300B1 (en) 2013-07-17 2018-07-11 Novozymes A/S Pullulanase chimeras and polynucleotides encoding same
CN103571812B (zh) * 2013-11-26 2015-06-24 江南大学 一种分泌效率和热稳定性提高的普鲁兰酶突变体及其制备方法
CN105960457B (zh) 2014-01-22 2021-09-03 诺维信公司 普鲁兰酶变体以及编码它们的多核苷酸
RU2019134734A (ru) 2014-02-07 2019-11-18 Новозимс А/С Композиции для получения глюкозных сиропов
CA2967710A1 (en) 2014-12-01 2016-06-09 Novozymes A/S Improved production of glucose syrups
WO2016087327A1 (en) 2014-12-01 2016-06-09 Novozymes A/S Polypeptides having pullulanase activity comprising the x25, x45 and cbm41 domains
WO2016126294A1 (en) 2015-02-04 2016-08-11 Nanjing Bestzyme Bio-Engineering Co. Ltd. Truncated pullulanases, methods of production, and methods of use thereof
US11427811B2 (en) 2015-07-21 2022-08-30 Novozymes A/S Polypeptides having pullulanase activity suitable for use in liquefaction
MX2018016282A (es) 2016-06-30 2019-04-15 Danisco Us Inc Proteasas asparticas.
EP3558026A1 (en) 2016-12-21 2019-10-30 DuPont Nutrition Biosciences ApS Methods of using thermostable serine proteases
RU2019132448A (ru) 2017-03-15 2021-04-15 ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС Способы применения сериновой протеазы архей
US11453871B2 (en) 2017-03-15 2022-09-27 Danisco Us Inc. Trypsin-like serine proteases and uses thereof
US20200015499A1 (en) 2017-03-15 2020-01-16 Dupont Nutrition Biosciences Aps Trypsin-like serine proteases and uses thereof
CN111032856A (zh) 2017-08-07 2020-04-17 诺维信公司 基于pH的FCA控制的用途
FR3091640B1 (fr) 2019-01-11 2021-06-11 Fermentalg Procédé de purification de phycocyanines
FR3091703B1 (fr) 2019-01-11 2021-02-12 Fermentalg Procédé d’extraction de phycocyanines
FR3092586A1 (fr) 2019-02-08 2020-08-14 Fermentalg Procédé optimisé d’exploitation industrielle d’algues rouges unicellulaires
CN113490740A (zh) 2019-03-18 2021-10-08 诺维信公司 适合用于液化中的具有普鲁兰酶活性的多肽
BR112022008292A2 (pt) 2019-11-08 2022-07-26 Novozymes As Composição enzimática líquida, e, método para produzir um mosto para fabricação de cerveja
CN111560077A (zh) * 2020-05-21 2020-08-21 中国海洋大学 一种酶及其在合成普鲁兰多糖中的作用
AU2023272468A1 (en) 2022-05-14 2024-11-14 Novonesis Plant Biosolutions A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
FR3147934A1 (fr) 2023-04-19 2024-10-25 Fermentalg Procede de degradation du glycogene present dans une biomasse de galdieria

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57174089A (en) * 1981-04-20 1982-10-26 Novo Industri As Chain dividing enzyme product
US4560651A (en) * 1981-04-20 1985-12-24 Novo Industri A/S Debranching enzyme product, preparation and use thereof
US4628031A (en) * 1984-09-18 1986-12-09 Michigan Biotechnology Institute Thermostable starch converting enzymes
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
US4628028A (en) * 1985-05-23 1986-12-09 Cpc International Inc. Novel thermostable pullulanase enzyme and method for its production
US4612287A (en) * 1985-05-23 1986-09-16 Cpc International Inc. Plasmids containing a gene coding for a thermostable pullulanase and pullulanase-producing strains of Escherichia coli and Bacillus subtilis containing the plasmids
US4902622A (en) * 1986-09-26 1990-02-20 Amano Pharmaceutical Co., Ltd. Novel α-1,6-glucosidase and process for producing the same
JPH0223872A (ja) * 1988-07-12 1990-01-26 Ezaki Glico Co Ltd 組換えプラスミド，それにより形質転換された枯草菌及びそれによる耐熱性プルラナーゼの製造法
US5055403A (en) * 1989-06-26 1991-10-08 Enzyme Bio-Systems, Ltd. Thermoduric and aciduric pullulanase enzyme and method for its production
EP0405283A3 (en) * 1989-06-26 1991-03-06 Enzyme Bio-Systems Ltd. Novel thermoduric and aciduric pullulanase enzyme and method for its production
DK47291D0 (da) * 1991-03-15 1991-03-15 Novo Nordisk As Pullulanase
JP3026857B2 (ja) * 1991-07-05 2000-03-27 ナガセ生化学工業株式会社 新規プルラナーゼおよびその製造法
JP3173849B2 (ja) * 1992-02-26 2001-06-04 天野エンザイム株式会社 新規な枝切り酵素及びその製造法
JPH05236959A (ja) * 1992-02-28 1993-09-17 Yoshiyuki Takasaki プルラナーゼ、その製造法及び該酵素を用いる澱粉の 糖化法
DE4244107C2 (de) * 1992-12-24 1996-02-08 Hirschmann Richard Gmbh Co Hochfrequenz-Übertrager
DK0605040T3 (da) * 1992-12-28 2000-03-13 Genencor Int Pullulanase, mikroorganismer producerende denne fremgangsmåde il fremstilling af denne pullulanase og anvendelse deraf
GB2279955B (en) * 1993-07-15 1998-02-18 Solvay Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof

Also Published As

Publication number Publication date
FI935900A0 (fi) 1993-12-28
KR100319442B1 (ko) 2002-11-18
CA2112028C (fr) 2003-03-11
AU6483198A (en) 1998-07-30
US5736375A (en) 1998-04-07
FI935900A (fi) 1994-06-29
ES2137222T3 (es) 1999-12-16
FI116531B (fi) 2005-12-15
DE69325981T2 (de) 2000-02-03
US6074854A (en) 2000-06-13
DE69325981D1 (de) 1999-09-16
CN1061089C (zh) 2001-01-24
CN1090325A (zh) 1994-08-03
AU5275993A (en) 1994-07-07
KR940014776A (ko) 1994-07-19
US5721128A (en) 1998-02-24
EP0605040B1 (fr) 1999-08-11
EP0605040A1 (fr) 1994-07-06
US5721127A (en) 1998-02-24
MX9400123A (es) 1994-07-29
CA2112028A1 (fr) 1994-06-29
JPH06217770A (ja) 1994-08-09
US5731174A (en) 1998-03-24
AU686574B2 (en) 1998-02-12
ATE183236T1 (de) 1999-08-15
US5817498A (en) 1998-10-06

Similar Documents

Publication Publication Date Title
DK0605040T3 (da) Pullulanase, mikroorganismer producerende denne fremgangsmåde il fremstilling af denne pullulanase og anvendelse deraf
ATE117019T1 (de) Neue hyperthermostabile alpha - amylase.
AU9737398A (en) Alpha-amylase mutants
ATE43634T1 (de) Maltose erzeugendes amylase-enzymprodukt, dessen herstellung und dessen verwendung.
BR8506273A (pt) Gene de glicoamilase,vetor compreendendo o mesmo,microorganismo transformado,e processo para produzir glicoamilase
PT673427E (pt) Processo para imobilizar enzimas na parede celular de uma celula microbiana atraves da producao de uma proteina de fusao
DE69534855D1 (de) Enzymaktive dna moleküle
KR950032615A (ko) 말토헥사오스 및 말토헵타오스 생성 아밀라아제와 그 제조 방법 및 용도
KR890013173A (ko) 시클로말토덱스트린 글루카노트랜스퍼라제 및 그의 제조방법
ATE395427T1 (de) Neue enzyme, welche glycerin entwässern
ATE234928T1 (de) Hitzebeständige rekombinante enzyme für die umsetzung von maltose in trehalose
AU576505B2 (en) Thermal and acid stable alpha amylase
IE791083L (en) Immobilised galactosidase enzyme
CA2154628A1 (fr) Xylanase, microorganismes la produisant, molecules d'adn, procedes de preparation de cette xylanase et utilisations de celle-ci
EP0273268A3 (en) Reduced-stability alpha-amylase and process for its production
DE69825926D1 (de) Gereinigte säurestabile Alpha-Amylase von schimmeliger Herkunft
DK41588A (da) Termostabile amylaser og pullulanaser
KR960023051A (ko) 말토테트라오즈 생산 아밀라제의 제조방법
GB2022595A (en) beta -Galactosidase and the use thereof
KR960034409A (ko) 신균주 프로테우스 불가리스(Proteus vulgaris) K80과 이로부터 생산되는 신규한 리파제(lipase)
KR950026986A (ko) 내열성 d-히단토이나제 활성을 갖는 고온성 신규 미생물을 이용한 n-카르바모일-d-아미노산의 제조 방법
DK161084A (da) Maltogent amylaseenzymprodukt og fremgangsmaade til dets fremstilling og anvendelse
YUTANI et al. Comparison of Thermostable α-Amylases from
KR970015746A (ko) 고정화 효소를 이용한 7-아미노-3-크로로-3-세펨-4-카르복실산 제조법
JPS57146590A (en) Preparation of oligosaccharide with amylase g6 belonging to genus bacillus