KR890013173A - 시클로말토덱스트린 글루카노트랜스퍼라제 및 그의 제조방법 - Google Patents

시클로말토덱스트린 글루카노트랜스퍼라제 및 그의 제조방법 Download PDF

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KR890013173A
KR890013173A KR1019890001328A KR890001328A KR890013173A KR 890013173 A KR890013173 A KR 890013173A KR 1019890001328 A KR1019890001328 A KR 1019890001328A KR 890001328 A KR890001328 A KR 890001328A KR 890013173 A KR890013173 A KR 890013173A
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cyclomaltodextrin glucanotransferase
culture
stable
molecular weight
hours
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KR1019890001328A
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KR910002852B1 (ko
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고끼 호리꼬시
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오다 미노루
리까가꾸 겡꾸쇼
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1074—Cyclomaltodextrin glucanotransferase (2.4.1.19)
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12N1/205—Bacterial isolates
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus

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Abstract

내용 없음.

Description

시클로말토덱스트린 글루카노트랜스피라제 및 그의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명의 CGT 아제 사용시 α,β, γ 및 총 CD의 시간-경과에 따른 변화를 나타낸다.
제2도 및 CGT 아제활성에 대한 pH효과 및 온도 효과를 각각 나타낸다.
제3도는 효소의 pH- 및 열-안정성의 프로필을 나타낸다.

Claims (5)

  1. (a) 작용 : 전분 및 글리코겐과 같은 α-1,4-글루칸, 또는 그의 부분 가수분해물의 α-1,4-글루코피라노시드 결합을 절단하고 그것을 시클로덱스트린을 형성하도록 전이시켜 CDs-생성반응의 초기 반응 생성물 로서 β-시클로덱스트린을 제조함; (b) 기질특이성 : 사슬 길이가 말토트리오스의 길이보다 짧지않은 α-1,4-글루코피라노시드 결합을 갖는 말토올리고사카라이드와 반응하여 기질간의 분자간 커플링 및 불균화 반응에 의해 다양한 분자량을 갖는 각종 말토올리고사카라이드 및 시클로덱스트린을 제조함; (c) 최적 pH 약 65 ; (d) 안정 pH pH 6∼9, 40℃에서 2시간동안 안정함 ; (e) 최적온도 : 약 65℃ ; (f) 불활성화 조건 : pH4.5 및 11.5, 40℃의 온도에서 2시간의 처리로 및 pH 6, 75℃에서 15분간의 처리로 완전히 불활성화됨 ; (g)열안정성 : pH 6의 조건하에서 5분동안 50℃까지 안정하며, 60℃ 및 70℃에서의 잔류활성이 동일조건하에서 각각 95% 및 20%임 ; (h) 억제 : 수은(II) 또는 구리(II)에 의해 억제됨. (i) 활성화 및 안정화 : 칼슘에 의해 안정화됨 ; (j) 분자량(SDS폴리아크릴아미드겔 전기영동) : 36000±1000 ; (k) 등전점(크로마토포커싱) : 4.8±0.1의 물리적 특성을 갖는 시클로말토덱스트린 글루카노트랜스퍼라제.
  2. 바실루스 코아굴란스(Bacillus cogulans)에 속하는 시클로말토덱스트린 글루카노트랜스퍼라제 생산 미생물을 배양하고 수득된 배양 브로쓰로부터 시클로말토덱스트린 글루카노트랜스퍼라제를 수집함을 특징으로하는 시클로말토덱스트린 글루카노트랜스퍼라제의 제조방법.
  3. 제2항에 있어서, 바실루스 코아굴란스에 속하는 시클로말토덱스트린 글루카노트랜스퍼라제 생산 미생물이 세균 FERM BP-2258인 제조방법.
  4. 제2항에 있어서, 배양을 30∼60℃에서 호기적으로 수행하는 제조방법.
  5. 제2항에 있어서, 배양을 위해 사용되는 배양액의 pH가 5.8∼7 5의 범위인 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890001328A 1988-02-04 1989-02-04 시클로말토덱스트린 글루카노트랜스퍼라제 및 그의 제조방법 KR910002852B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP63024478A JPH01199575A (ja) 1988-02-04 1988-02-04 新規なサイクロマルトデキストリン・グルカノトランスフェラーゼ及びその製造法
JP63-24478 1988-02-04
JP88-24478 1988-02-04

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KR890013173A true KR890013173A (ko) 1989-09-21
KR910002852B1 KR910002852B1 (ko) 1991-05-06

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US (2) US5019507A (ko)
EP (1) EP0327099B1 (ko)
JP (1) JPH01199575A (ko)
KR (1) KR910002852B1 (ko)
DE (1) DE68909625T2 (ko)
DK (1) DK50889A (ko)

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JP3060232B2 (ja) * 1990-04-29 2000-07-10 株式会社林原生物化学研究所 α―グリコシル ナリンジンとその製造方法並びに用途
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JP2896598B2 (ja) * 1990-10-06 1999-05-31 株式会社林原生物化学研究所 ラクトネオトレハロースとその製造方法並びに用途
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US5102800A (en) 1992-04-07
EP0327099B1 (en) 1993-10-06
EP0327099A2 (en) 1989-08-09
DK50889D0 (da) 1989-02-03
DE68909625D1 (de) 1993-11-11
EP0327099A3 (en) 1990-06-27
JPH01199575A (ja) 1989-08-10
DE68909625T2 (de) 1994-05-05
DK50889A (da) 1989-08-05
US5019507A (en) 1991-05-28
KR910002852B1 (ko) 1991-05-06

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