GB2022595A - beta -Galactosidase and the use thereof - Google Patents

beta -Galactosidase and the use thereof

Info

Publication number
GB2022595A
GB2022595A GB7919738A GB7919738A GB2022595A GB 2022595 A GB2022595 A GB 2022595A GB 7919738 A GB7919738 A GB 7919738A GB 7919738 A GB7919738 A GB 7919738A GB 2022595 A GB2022595 A GB 2022595A
Authority
GB
United Kingdom
Prior art keywords
beta
galactosidase
enzyme
lactose
species
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB7919738A
Other versions
GB2022595B (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GRIFFITHS M W MUIR D
Original Assignee
GRIFFITHS M W MUIR D
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GRIFFITHS M W MUIR D filed Critical GRIFFITHS M W MUIR D
Priority to GB7919738A priority Critical patent/GB2022595B/en
Publication of GB2022595A publication Critical patent/GB2022595A/en
Application granted granted Critical
Publication of GB2022595B publication Critical patent/GB2022595B/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A beta -galactosidase comprises an enzyme having a thermal stability such that when in purified form it has an activity half life of at least 11 DIVIDED 2 hours at 55 DEG C, at least 1 hour at 60 DEG C and at least 10 minutes at 65 DEG C, as measured using ONPG as substrate, said enzyme being derived from a bacterium of species Bacillus stearothermophilus. There is also provided a process for the production of the said beta -galactosidase, comprising culturing in a medium containing appropriate nutrients a micro- organism of species B. stearothermophilus having characteristics similar to or the same as the strains identified as NCIB 11407-11413, and which contains the said beta -Galactosidase. Additionally there is provided a process for the hydrolysis of lactose to a product comprising glucose and galactose which comprises contacting lactose with the said beta -galactosidase; e.g. at a temperature of at least 55 DEG C, preferably at least 60 DEG C or especially about 65 DEG C.
GB7919738A 1978-06-07 1979-06-06 S-galactosidase and the use thereof Expired GB2022595B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB7919738A GB2022595B (en) 1978-06-07 1979-06-06 S-galactosidase and the use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB7826503 1978-06-07
GB7919738A GB2022595B (en) 1978-06-07 1979-06-06 S-galactosidase and the use thereof

Publications (2)

Publication Number Publication Date
GB2022595A true GB2022595A (en) 1979-12-19
GB2022595B GB2022595B (en) 1982-08-25

Family

ID=26267928

Family Applications (1)

Application Number Title Priority Date Filing Date
GB7919738A Expired GB2022595B (en) 1978-06-07 1979-06-06 S-galactosidase and the use thereof

Country Status (1)

Country Link
GB (1) GB2022595B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0046614A1 (en) * 1980-08-22 1982-03-03 Région Wallonne représentée par l'Exécutif Régional Wallon Process for the immobilisation of globular microbial cells by means of adhesion to a solid support

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0046614A1 (en) * 1980-08-22 1982-03-03 Région Wallonne représentée par l'Exécutif Régional Wallon Process for the immobilisation of globular microbial cells by means of adhesion to a solid support

Also Published As

Publication number Publication date
GB2022595B (en) 1982-08-25

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Legal Events

Date Code Title Description
732 Registration of transactions, instruments or events in the register (sect. 32/1977)
PCNP Patent ceased through non-payment of renewal fee