KR940014776A - 플루라아나제, 이를 제조하는 미생물, 이 플루라나아제의 제조방법과 이의 용도 - Google Patents

플루라아나제, 이를 제조하는 미생물, 이 플루라나아제의 제조방법과 이의 용도 Download PDF

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KR940014776A
KR940014776A KR1019930030410A KR930030410A KR940014776A KR 940014776 A KR940014776 A KR 940014776A KR 1019930030410 A KR1019930030410 A KR 1019930030410A KR 930030410 A KR930030410 A KR 930030410A KR 940014776 A KR940014776 A KR 940014776A
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flulanase
bacillus
vector
deramipicans
strain
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드위어 필립
아모리 안토인
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피. 안토니
솔베이(소시에떼 아노님)
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Abstract

본 발명은 아미로펙틴에서 α-1.6형의 글루코시드 결합을 가수분해하는 성질을 갖고 산성배지에서와 약 60℃의 온도에서 효소활성을 갖는 열안정성 플루라나아제에 관한 것이다. 또한, 본 발명은 이 플루라나아제를 생성시키는 미생물의 균주와 이 플루라나아제의 제조방법에 관한 것이다. 또한 본 발명은 DNA분자에 관한 것이고, 본 발명은 이 DNA분자를 함유하는 합성벡터와 이 DNA분자를 함유 하는 염색체 집성벡터에 관한 것이다.

Description

플루라아나제, 이를 제조하는 미생물, 이 플루라나아제의 제조방법과 이의 용도
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 플라스미드 pUBDEBRA1의 제한효소지도를 나타내고,
제2도는 플라스미드 pLD1을 제한효소지도를 나타내며,
제3도는 플라스미드 pUBCDEBR11DNSI의 제한효소지도를 나타낸다.

Claims (20)

  1. 균주 바실러스 데라미피칸스에 의하여 또는 이 균주의 유도체나 돌연변이체에 의하여 제조됨을 특징으로 하는 플루라나아제.
  2. N-말단 배열(SEQ ID NO:1)이 다음과 같이 아미노 카르복실 감응에서와 좌에서 우로 위치함을 특징으로 하는 플루라나아제:
  3. 제4도 (SEQ ID NO :11)에 예시된 바와같은 1~928아미노산의 아미노산 배열 또는 이로부터 유도된 수정배열로 이루어짐을 특징으로 하는 단리되고 정제된 플루라나아제.
  4. 299아미노산의 부가적배열(SEQ ID NO:12)을 함유하는 전구물질의 형태로 합성함을 특징으로 하는 플루라나아제.
  5. 야생상태의 알칼리성 플로테아제를 부호화하는 유전자를 함유하는 바실러스속의 미생물로 이종방법에 따라 바실러속의 미생물에서 삭제된 알칼리성 플로테아제를 부호화 하는 유전자를 제조함을 특징으로 하는 플루라나아제.
  6. 호기성 조건하에서 탄소 및 질소급원과 무기염을 함유하는 적당한 배양기에서 플루라나아제를 제조하고 얻은 플루라나아제를 수확할 수 있는 균주 바실러스 데라미피칸스 또는 이 균주의 유도체의 배양으로 이루어짐을 특징으로 하는 제1항 내지 제4항중 어느 한 항에 따른 플루라나아제의 제조방법.
  7. 플루라나아제를 부호화하는 DNA단편의 단리, 이 DNA단편을 적당한 벡터에 삽입, 이 벡터를 적당한 숙주에 주입 또는 이 DNA단편을 적당한 숙주의 염색체에 주입, 이 숙주의 배양, 플루라나아제의 합성과 플루라나아제의 수확으로 이루어짐을 특징으로 하는 제1항 내지 제4항중 어느 한 항에 따른 플루라나아제의 제조방법.
  8. 전분의 당화에 사용하는 제1항 내지 제5항중 어느 한 항에 따른 플루라나아제의 용도.
  9. 바실러스 데라미피칸스의 플루라나아제를 부호화하는 누클레오티드 배열(SEQ ID NO:10)또는 이러부터 유도된 수정배열로 이루어지는 DNA분자.
  10. 제9항에 있어서, 바실러스 데라미피칸스 T89.117D의 플루라나아제의 전체 유전자 (SEQ ID NO:8)로 이루어짐을 특징으로 하는 DNA 분자.
  11. 제9항에 따른 DNA분자를 함유하는 합성벡터 또는 염색체 집성백터.
  12. 합성벡터 pUBDEBRA1
  13. DUATORCP 집성벡터 pUBDEBRA11DNSI
  14. 제9항에 따른 DNA분자를 함유하는 바실러스 리케니포르미스의 형질전환된 균주.
  15. 제11항에 따른 합성벡터 또는 염색체 집성벡터를 함유하는 바실러스 리케니포르미스의 형질전환된 균주.
  16. 합성벡터 pUBDEBRA1 또는 염색체 집성벡터 pUBDEBRA11DNSI를 함유하는 바실러스 리케니포르미스의 형질전환된 균주.
  17. 제14항, 제15항, 또는 제16항에 따른 바실러스 리케니포르미스의 형질 전환된 균주에 의하여 플루라나아제.
  18. 바실러스 데라미피칸스의 단리되고 정제된 배양물과 이러부터 유도되거나 돌연변이된 배양물.
  19. 균주 바실러스 데라미피칸스 T 89.117D(LMG P-13056) 또는 이 균주의 유도체나 돌연변이체에 의하여 제조됨을 특징으로 하는 플라라나아제.
  20. 바실러스 데라미피칸스 T 89 .117D의 단리되고 정제된 배양물과 이로부터 유도되거나 돌연변이된 배양물.
    ※참고사항:최초출원 내용에 의하여 공개하는 것임.
KR1019930030410A 1992-12-28 1993-12-28 플루라나아제, 이를 제조하는 미생물, 이플루라나아제의 제조방법과 이의 용도 KR100319442B1 (ko)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
BE9201156 1992-12-28
BE9201156A BE1006483A3 (fr) 1992-12-28 1992-12-28 Pullulanase, microorganismes la produisant, procedes de preparation de cette pullulanase, utilisations et compositions comprenant celle-ci.
BE9300744A BE1007313A3 (fr) 1993-07-15 1993-07-15 Pullulanase, microorganismes la produisant, procedes de preparation de cette pullulanase et utilisations de celle-ci.
BE9300744 1993-07-15
BE9301278A BE1007723A6 (fr) 1993-11-19 1993-11-19 Pullulanase, microorganismes la produisant, procedes de preparation de cette pullulanase et utilisations de celle-ci.
BE09301278 1993-11-19
BE09300744 1993-11-19
BE9301278 1993-11-19
BE09201156 1993-11-19

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KR940014776A true KR940014776A (ko) 1994-07-19
KR100319442B1 KR100319442B1 (ko) 2002-11-18

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Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE183236T1 (de) * 1992-12-28 1999-08-15 Genencor Int Pullulanase, mikroorganismen die sie produzieren, verfahren zur herstellung und ihre anwendung
AU714478B2 (en) * 1995-09-13 2000-01-06 Genencor International, Inc. Alkaliphilic and thermophilic microorganisms and enzymes obtained therefrom
US6962263B2 (en) 1996-01-24 2005-11-08 Sambrailo Packaging, Inc. Produce packaging system having produce containers with double-arched ventilation channels
DE19627932A1 (de) * 1996-07-11 1998-01-15 Boehringer Mannheim Gmbh Sensitiver Epstein-Barr-Virus DNA-Nachweis
US7279316B2 (en) * 1996-12-06 2007-10-09 Verenium Corporation Enzymes having glycosidase activity and methods of use thereof
PT1027367E (pt) 1996-12-06 2005-09-30 Diversa Corp Enzimas de glicosidade
JP2002505108A (ja) 1998-03-04 2002-02-19 ジェネンコア インターナショナル インコーポレーテッド プルラナーゼの修飾形態
US6639126B1 (en) 1999-12-06 2003-10-28 Pioneer Hi-Bred International, Inc. Production of modified polysaccharides
US6350599B1 (en) 2000-01-12 2002-02-26 Novozymes A/S Pullulanase variants and methods for preparing such variants with predetermined properties
US7081261B2 (en) * 2002-05-14 2006-07-25 National Starch And Chemical Investment Holding Corporation Resistant starch prepared by isoamylase debranching of low amylose starch
US20050233030A1 (en) * 2004-03-10 2005-10-20 Broin And Associates, Inc. Methods and systems for producing ethanol using raw starch and fractionation
EP1603406B1 (en) * 2003-03-10 2012-10-24 POET Research, Inc. Method for producing ethanol using raw starch
EP1662898A4 (en) * 2003-09-08 2010-09-08 Univ Louisiana State RESISTANT STRENGTH WITH COOKING CHARACTERISTICS LIKE UNKNOWN STRENGTH
US20050239181A1 (en) * 2004-03-10 2005-10-27 Broin And Associates, Inc. Continuous process for producing ethanol using raw starch
WO2006119386A2 (en) * 2005-05-02 2006-11-09 Broin And Associates, Inc. Methods and systems for producing ethanol using raw starch and fractionation
US8216817B2 (en) 2005-09-20 2012-07-10 Novozymes North America, Inc. Process of producing a fermentation product
US7919289B2 (en) * 2005-10-10 2011-04-05 Poet Research, Inc. Methods and systems for producing ethanol using raw starch and selecting plant material
WO2007144393A1 (en) * 2006-06-15 2007-12-21 Novozymes, Inc. Mashing process
US8765199B2 (en) 2006-06-15 2014-07-01 Novozymes A/S Mashing process
US9969996B2 (en) 2006-08-04 2018-05-15 Amano Enzyme Inc. Method for making mutated pullulanase enzyme, mutated pullulanase enzyme, and microorganism expressing the same
EP2058394B1 (en) * 2006-08-04 2014-11-12 Amano Enzyme Inc. Method for designing mutated enzyme, method for preparing the same and mutated enzyme
US7968691B2 (en) * 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity
WO2009074650A2 (en) 2007-12-12 2009-06-18 Novozymes A/S Brewing process
CN105623937A (zh) * 2007-12-12 2016-06-01 诺维信公司 糖化方法
EP2346978A2 (en) 2008-10-15 2011-07-27 Novozymes A/S Brewing process
WO2010102063A2 (en) * 2009-03-03 2010-09-10 Poet Research, Inc. System for fermentation of biomass for the production of ethanol
US9068206B1 (en) 2009-03-03 2015-06-30 Poet Research, Inc. System for treatment of biomass to facilitate the production of ethanol
US8450094B1 (en) 2009-03-03 2013-05-28 Poet Research, Inc. System for management of yeast to facilitate the production of ethanol
WO2010102060A2 (en) * 2009-03-03 2010-09-10 Poet Research, Inc. System for pre-treatment of biomass for the production of ethanol
EP2430176A2 (en) * 2009-05-11 2012-03-21 Danisco US Inc. Improved production of maltotetraose syrup using a pseudomonas saccharophila maltotetraohydrolase variant and a debranching enzyme
MX2012001243A (es) 2009-08-07 2012-03-26 Danisco Us Inc Combinacion de alfa-amilasa para procesamiento de almidon y metodo de uso de la misma.
CN102639687A (zh) 2009-11-13 2012-08-15 诺维信公司 酿造方法
MX357536B (es) 2011-04-15 2018-07-13 Novozymes As Metodo para la produccion de mosto de la cerveza.
JP2014528241A (ja) 2011-09-29 2014-10-27 ダニスコ・ユーエス・インク 高濃度デンプン粒の液化及び糖化
MX353581B (es) 2011-10-11 2018-01-19 Novozymes North America Inc Procesos para producir productos de fermentacion.
WO2013148152A1 (en) 2012-03-28 2013-10-03 Danisco Us Inc. Method for making high maltose syrup
BR112014027861A2 (pt) 2012-05-11 2017-12-12 Novozymes As método de preparação de um mosto
MX2015001818A (es) 2012-08-16 2015-05-07 Danisco Inc Proceso para producir glucosa a partir de almidon con el uso de la alfa-amilasa de aspergillus clavatus y una pululanasa.
CN104718289A (zh) 2012-10-17 2015-06-17 诺维信公司 用于生产啤酒麦芽汁的方法
EP3321353A1 (en) 2012-12-11 2018-05-16 Danisco US Inc. Yeast host cells epxressing a glucoamylase from aspergillus fumigatus and methods of use thereof
WO2014099415A1 (en) 2012-12-20 2014-06-26 Danisco Us Inc. Method of using alpha-amylase from aspergillus terreus and pullulanase for saccharification
WO2014150195A1 (en) * 2013-03-15 2014-09-25 Verenium Corporation Enzymes having pullulanase activity
CA2915336C (en) 2013-07-17 2024-03-12 Novozymes A/S Pullulanase chimeras and polynucleotides encoding same
CN103571812B (zh) * 2013-11-26 2015-06-24 江南大学 一种分泌效率和热稳定性提高的普鲁兰酶突变体及其制备方法
EP3415624B1 (en) 2014-01-22 2021-08-04 Novozymes A/S Pullulanase variants and polynucleotides encoding same
EP3712240B1 (en) 2014-02-07 2023-09-27 Novozymes A/S Compositions for producing glucose syrups
MX2017007102A (es) 2014-12-01 2017-08-24 Novozymes As Produccion mejorada de jarabes de glucosa.
WO2016087327A1 (en) 2014-12-01 2016-06-09 Novozymes A/S Polypeptides having pullulanase activity comprising the x25, x45 and cbm41 domains
JP6505240B2 (ja) 2015-02-04 2019-04-24 ナンジン ベストザイム バイオ−エンジニアリング コンパニー リミテッド 切断型プルラナーゼの製造および使用方法
US11427811B2 (en) 2015-07-21 2022-08-30 Novozymes A/S Polypeptides having pullulanase activity suitable for use in liquefaction
CN109642226A (zh) 2016-06-30 2019-04-16 丹尼斯科美国公司 天冬氨酸蛋白酶
WO2018118815A1 (en) 2016-12-21 2018-06-28 Dupont Nutrition Biosciences Aps Methods of using thermostable serine proteases
WO2018169784A1 (en) 2017-03-15 2018-09-20 Dupont Nutrition Biosciences Aps Trypsin-like serine proteases and uses thereof cross-reference to related application
WO2018169750A1 (en) 2017-03-15 2018-09-20 Danisco Us Inc Trypsin-like serine proteases and uses thereof
DK3596211T3 (da) 2017-03-15 2021-09-06 Dupont Nutrition Biosci Aps Fremgangsmåder til anvendelse af en archaea-serinprotease
US11667980B2 (en) 2017-08-07 2023-06-06 Novozymes A/S Use of FCA control based on PH
FR3091703B1 (fr) 2019-01-11 2021-02-12 Fermentalg Procédé d’extraction de phycocyanines
FR3091640B1 (fr) 2019-01-11 2021-06-11 Fermentalg Procédé de purification de phycocyanines
FR3092586A1 (fr) 2019-02-08 2020-08-14 Fermentalg Procédé optimisé d’exploitation industrielle d’algues rouges unicellulaires
WO2020187883A1 (en) 2019-03-18 2020-09-24 Novozymes A/S Polypeptides having pullulanase activity suitable for use in liquefaction
US20220372461A1 (en) 2019-11-08 2022-11-24 Novozymes A/S Stabilized liquid enzyme compositions for brewing
CN111560077A (zh) * 2020-05-21 2020-08-21 中国海洋大学 一种酶及其在合成普鲁兰多糖中的作用
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57174089A (en) * 1981-04-20 1982-10-26 Novo Industri As Chain dividing enzyme product
US4560651A (en) * 1981-04-20 1985-12-24 Novo Industri A/S Debranching enzyme product, preparation and use thereof
US4628031A (en) * 1984-09-18 1986-12-09 Michigan Biotechnology Institute Thermostable starch converting enzymes
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
US4612287A (en) * 1985-05-23 1986-09-16 Cpc International Inc. Plasmids containing a gene coding for a thermostable pullulanase and pullulanase-producing strains of Escherichia coli and Bacillus subtilis containing the plasmids
US4628028A (en) * 1985-05-23 1986-12-09 Cpc International Inc. Novel thermostable pullulanase enzyme and method for its production
US4902622A (en) * 1986-09-26 1990-02-20 Amano Pharmaceutical Co., Ltd. Novel α-1,6-glucosidase and process for producing the same
JPH0223872A (ja) * 1988-07-12 1990-01-26 Ezaki Glico Co Ltd 組換えプラスミド，それにより形質転換された枯草菌及びそれによる耐熱性プルラナーゼの製造法
EP0405283A3 (en) * 1989-06-26 1991-03-06 Enzyme Bio-Systems Ltd. Novel thermoduric and aciduric pullulanase enzyme and method for its production
US5055403A (en) * 1989-06-26 1991-10-08 Enzyme Bio-Systems, Ltd. Thermoduric and aciduric pullulanase enzyme and method for its production
DK47291D0 (da) * 1991-03-15 1991-03-15 Novo Nordisk As Pullulanase
JP3026857B2 (ja) * 1991-07-05 2000-03-27 ナガセ生化学工業株式会社 新規プルラナーゼおよびその製造法
JP3173849B2 (ja) * 1992-02-26 2001-06-04 天野エンザイム株式会社 新規な枝切り酵素及びその製造法
JPH05236959A (ja) * 1992-02-28 1993-09-17 Yoshiyuki Takasaki プルラナーゼ、その製造法及び該酵素を用いる澱粉の 糖化法
DE4244107C2 (de) * 1992-12-24 1996-02-08 Hirschmann Richard Gmbh Co Hochfrequenz-Übertrager
ATE183236T1 (de) * 1992-12-28 1999-08-15 Genencor Int Pullulanase, mikroorganismen die sie produzieren, verfahren zur herstellung und ihre anwendung
GB2279955B (en) * 1993-07-15 1998-02-18 Solvay Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof

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DK0605040T3 (da) 2000-03-13
US5736375A (en) 1998-04-07
US5721127A (en) 1998-02-24
FI116531B (fi) 2005-12-15
DE69325981D1 (de) 1999-09-16
AU6483198A (en) 1998-07-30
AU686574B2 (en) 1998-02-12
MX9400123A (es) 1994-07-29
US5721128A (en) 1998-02-24
ES2137222T3 (es) 1999-12-16
FI935900A (fi) 1994-06-29
US6074854A (en) 2000-06-13
EP0605040B1 (fr) 1999-08-11
ATE183236T1 (de) 1999-08-15
DE69325981T2 (de) 2000-02-03
US5817498A (en) 1998-10-06
CA2112028C (fr) 2003-03-11
FI935900A0 (fi) 1993-12-28
KR100319442B1 (ko) 2002-11-18
EP0605040A1 (fr) 1994-07-06
US5731174A (en) 1998-03-24
CN1090325A (zh) 1994-08-03
JPH06217770A (ja) 1994-08-09
AU5275993A (en) 1994-07-07
CN1061089C (zh) 2001-01-24

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