ATE183236T1 - Pullulanase, mikroorganismen die sie produzieren, verfahren zur herstellung und ihre anwendung - Google Patents

Pullulanase, mikroorganismen die sie produzieren, verfahren zur herstellung und ihre anwendung

Info

Publication number
ATE183236T1
ATE183236T1 AT93203593T AT93203593T ATE183236T1 AT E183236 T1 ATE183236 T1 AT E183236T1 AT 93203593 T AT93203593 T AT 93203593T AT 93203593 T AT93203593 T AT 93203593T AT E183236 T1 ATE183236 T1 AT E183236T1
Authority
AT
Austria
Prior art keywords
pullulanase
relates
dna molecule
microorganisms
produce
Prior art date
Application number
AT93203593T
Other languages
English (en)
Inventor
Philippe Deweer
Antoine Amory
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from BE9201156A external-priority patent/BE1006483A3/fr
Priority claimed from BE9300744A external-priority patent/BE1007313A3/fr
Priority claimed from BE9301278A external-priority patent/BE1007723A6/fr
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of ATE183236T1 publication Critical patent/ATE183236T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Mechanical Means For Catching Fish (AREA)
  • Selective Calling Equipment (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
AT93203593T 1992-12-28 1993-12-20 Pullulanase, mikroorganismen die sie produzieren, verfahren zur herstellung und ihre anwendung ATE183236T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
BE9201156A BE1006483A3 (fr) 1992-12-28 1992-12-28 Pullulanase, microorganismes la produisant, procedes de preparation de cette pullulanase, utilisations et compositions comprenant celle-ci.
BE9300744A BE1007313A3 (fr) 1993-07-15 1993-07-15 Pullulanase, microorganismes la produisant, procedes de preparation de cette pullulanase et utilisations de celle-ci.
BE9301278A BE1007723A6 (fr) 1993-11-19 1993-11-19 Pullulanase, microorganismes la produisant, procedes de preparation de cette pullulanase et utilisations de celle-ci.

Publications (1)

Publication Number Publication Date
ATE183236T1 true ATE183236T1 (de) 1999-08-15

Family

ID=27159793

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93203593T ATE183236T1 (de) 1992-12-28 1993-12-20 Pullulanase, mikroorganismen die sie produzieren, verfahren zur herstellung und ihre anwendung

Country Status (13)

Country Link
US (6) US5721128A (de)
EP (1) EP0605040B1 (de)
JP (1) JPH06217770A (de)
KR (1) KR100319442B1 (de)
CN (1) CN1061089C (de)
AT (1) ATE183236T1 (de)
AU (2) AU686574B2 (de)
CA (1) CA2112028C (de)
DE (1) DE69325981T2 (de)
DK (1) DK0605040T3 (de)
ES (1) ES2137222T3 (de)
FI (1) FI116531B (de)
MX (1) MX9400123A (de)

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CN1199424A (zh) * 1995-09-13 1998-11-18 金克克国际有限公司 嗜碱和嗜热微生物及由其所获得的酶
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US6350599B1 (en) 2000-01-12 2002-02-26 Novozymes A/S Pullulanase variants and methods for preparing such variants with predetermined properties
US7081261B2 (en) * 2002-05-14 2006-07-25 National Starch And Chemical Investment Holding Corporation Resistant starch prepared by isoamylase debranching of low amylose starch
US20050233030A1 (en) * 2004-03-10 2005-10-20 Broin And Associates, Inc. Methods and systems for producing ethanol using raw starch and fractionation
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CN105623937A (zh) * 2007-12-12 2016-06-01 诺维信公司 糖化方法
US20110195149A1 (en) 2008-10-15 2011-08-11 Novozymes A/S Brewing process
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US8545907B2 (en) 2009-08-07 2013-10-01 Danisco Us Inc. Alpha-amylase blend for starch processing and method of use thereof
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DK2697356T3 (da) 2011-04-15 2020-09-07 Novozymes As Fremgangsmåde til fremstilling af ølurt
WO2013048700A1 (en) 2011-09-29 2013-04-04 Danisco Us Inc. Liquefaction and saccharification of granular starch at high concentration
ES2627987T3 (es) 2011-10-11 2017-08-01 Novozymes North America, Inc. Proceso de licuefacción para producir productos de fermentación
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WO2014028434A2 (en) 2012-08-16 2014-02-20 Danisco Us Inc. Method of using alpha-amylase from aspergillus clavatus and pullulanase for saccharification
CN104718289A (zh) 2012-10-17 2015-06-17 诺维信公司 用于生产啤酒麦芽汁的方法
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CN104903461A (zh) 2012-12-20 2015-09-09 丹尼斯科美国公司 将支链淀粉酶和来自土曲霉的α-淀粉酶用于糖化的方法
EP2970932B1 (de) * 2013-03-15 2018-11-14 BASF Enzymes LLC Enzyme mit pullulanaseaktivität
CA2915336C (en) 2013-07-17 2024-03-12 Novozymes A/S Pullulanase chimeras and polynucleotides encoding same
CN103571812B (zh) * 2013-11-26 2015-06-24 江南大学 一种分泌效率和热稳定性提高的普鲁兰酶突变体及其制备方法
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EP3325617B1 (de) 2015-07-21 2021-10-27 Novozymes A/S Polypeptide mit pullulanaseaktivität zur verwendung in der verflüssigung
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EP3558026B1 (de) 2016-12-21 2025-10-15 International N&H Denmark ApS Verfahren zur verwendung von thermostabilen serinproteasen
EP3596210A1 (de) 2017-03-15 2020-01-22 DuPont Nutrition Biosciences ApS Trypsinähnliche serinproteasen und verwendungen davon
US11453871B2 (en) 2017-03-15 2022-09-27 Danisco Us Inc. Trypsin-like serine proteases and uses thereof
DK3596211T3 (da) 2017-03-15 2021-09-06 Dupont Nutrition Biosci Aps Fremgangsmåder til anvendelse af en archaea-serinprotease
BR112020002605A2 (pt) 2017-08-07 2020-07-28 Novozymes A/S uso de controle de fca com base em ph
FR3091640B1 (fr) 2019-01-11 2021-06-11 Fermentalg Procédé de purification de phycocyanines
FR3091703B1 (fr) 2019-01-11 2021-02-12 Fermentalg Procédé d’extraction de phycocyanines
FR3092586A1 (fr) 2019-02-08 2020-08-14 Fermentalg Procédé optimisé d’exploitation industrielle d’algues rouges unicellulaires
MX2021011166A (es) 2019-03-18 2021-10-22 Novozymes As Polipeptidos que tienen actividad pululanasa adecuados para usarse en licuefaccion.
BR112022008292A2 (pt) 2019-11-08 2022-07-26 Novozymes As Composição enzimática líquida, e, método para produzir um mosto para fabricação de cerveja
CN111560077A (zh) * 2020-05-21 2020-08-21 中国海洋大学 一种酶及其在合成普鲁兰多糖中的作用
PE20251535A1 (es) 2022-05-14 2025-06-05 Novozymes As Composiciones y metodos para prevenir, tratar, suprimir y/o eliminar infecciones e infestaciones fitopatogenas
FR3147934A1 (fr) 2023-04-19 2024-10-25 Fermentalg Procede de degradation du glycogene present dans une biomasse de galdieria

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Also Published As

Publication number Publication date
MX9400123A (es) 1994-07-29
DE69325981T2 (de) 2000-02-03
FI935900L (fi) 1994-06-29
US5736375A (en) 1998-04-07
EP0605040B1 (de) 1999-08-11
FI116531B (fi) 2005-12-15
ES2137222T3 (es) 1999-12-16
CN1090325A (zh) 1994-08-03
CA2112028A1 (fr) 1994-06-29
CA2112028C (fr) 2003-03-11
US5721127A (en) 1998-02-24
KR940014776A (ko) 1994-07-19
US6074854A (en) 2000-06-13
US5731174A (en) 1998-03-24
JPH06217770A (ja) 1994-08-09
US5817498A (en) 1998-10-06
AU686574B2 (en) 1998-02-12
KR100319442B1 (ko) 2002-11-18
CN1061089C (zh) 2001-01-24
FI935900A0 (fi) 1993-12-28
US5721128A (en) 1998-02-24
EP0605040A1 (de) 1994-07-06
AU6483198A (en) 1998-07-30
DK0605040T3 (da) 2000-03-13
AU5275993A (en) 1994-07-07
DE69325981D1 (de) 1999-09-16

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