PT673427E - Processo para imobilizar enzimas na parede celular de uma celula microbiana atraves da producao de uma proteina de fusao - Google Patents

Processo para imobilizar enzimas na parede celular de uma celula microbiana atraves da producao de uma proteina de fusao

Info

Publication number
PT673427E
PT673427E PT93915827T PT93915827T PT673427E PT 673427 E PT673427 E PT 673427E PT 93915827 T PT93915827 T PT 93915827T PT 93915827 T PT93915827 T PT 93915827T PT 673427 E PT673427 E PT 673427E
Authority
PT
Portugal
Prior art keywords
protein
cell wall
pct
enzyme
cell
Prior art date
Application number
PT93915827T
Other languages
English (en)
Inventor
Cornelis Theodorus Verrips
Franciscus Maria Klis
Maarten Pleun Schreuder
Holger York Toschka
Original Assignee
Unilever Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever Nv filed Critical Unilever Nv
Publication of PT673427E publication Critical patent/PT673427E/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/16—Enzymes or microbial cells immobilised on or in a biological cell
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
PT93915827T 1992-07-08 1993-07-07 Processo para imobilizar enzimas na parede celular de uma celula microbiana atraves da producao de uma proteina de fusao PT673427E (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP92202080 1992-07-08
EP92203899 1992-12-14

Publications (1)

Publication Number Publication Date
PT673427E true PT673427E (pt) 2003-08-29

Family

ID=26131543

Family Applications (1)

Application Number Title Priority Date Filing Date
PT93915827T PT673427E (pt) 1992-07-08 1993-07-07 Processo para imobilizar enzimas na parede celular de uma celula microbiana atraves da producao de uma proteina de fusao

Country Status (16)

Country Link
US (1) US6027910A (pt)
EP (1) EP0673427B1 (pt)
JP (2) JP3681385B2 (pt)
KR (1) KR950702634A (pt)
AT (1) ATE236257T1 (pt)
AU (1) AU685057B2 (pt)
CA (1) CA2139670C (pt)
DE (1) DE69332829T2 (pt)
DK (1) DK0673427T3 (pt)
ES (1) ES2196009T3 (pt)
FI (1) FI950042A (pt)
NZ (1) NZ254108A (pt)
OA (1) OA10123A (pt)
PT (1) PT673427E (pt)
RU (1) RU95105899A (pt)
WO (1) WO1994001567A1 (pt)

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US5866344A (en) * 1991-11-15 1999-02-02 Board Of Regents, The University Of Texas System Antibody selection methods using cell surface expressed libraries
US20030036092A1 (en) * 1991-11-15 2003-02-20 Board Of Regents, The University Of Texas System Directed evolution of enzymes and antibodies
PT682710E (pt) * 1993-02-10 2004-03-31 Unilever Nv Processo de isolamento utilizando proteinas imobilizadas com capacidades de ligacao especificas
US5585271A (en) * 1993-02-26 1996-12-17 Sapporo Breweries Ltd. Yeast agglutination genes and yeast containing them
GB9423367D0 (en) * 1994-11-18 1995-01-11 Wellcome Found Enzyme prodrug therapy
US6300065B1 (en) 1996-05-31 2001-10-09 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6696251B1 (en) 1996-05-31 2004-02-24 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6699658B1 (en) 1996-05-31 2004-03-02 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6759243B2 (en) 1998-01-20 2004-07-06 Board Of Trustees Of The University Of Illinois High affinity TCR proteins and methods
AU778477B2 (en) 1999-03-25 2004-12-09 Valtion Teknillinen Tutkimuskeskus Process for partitioning of proteins
JP2003284556A (ja) * 1999-11-19 2003-10-07 Toyota Motor Corp 金属捕捉能タンパク質を発現する遺伝子
EP1134231B1 (en) 2000-03-14 2009-04-15 Unilever N.V. Antibody heavy chain variable domains against human dietary lipases, and their uses
DE10016279A1 (de) * 2000-04-03 2001-10-04 Henkel Kgaa Enzymatisches Färbemittel
ATE403717T1 (de) * 2000-05-01 2008-08-15 Daiichi Sankyo Co Ltd Verfahren zum screening von auf zellwände wirkenden arzneistoffen
GB2377932B (en) * 2000-05-15 2004-04-28 Schlumberger Holdings Permeable cements
CA2415569A1 (en) 2000-07-07 2002-01-17 Eisai Co., Ltd. Fungal cell wall synthesis gene
WO2002046400A2 (en) * 2000-12-08 2002-06-13 The Board Of Trustees Of The University Of Illinois Mutated class ii major histocompatibility proteins
DK1380592T3 (da) * 2001-04-19 2009-01-26 Kansai Chem Eng Celleoverfladelagsbindende protein og anvendelse heraf
WO2002101026A2 (en) 2001-06-11 2002-12-19 Applied Nanosystems B.V. Methods for binding acma-type protein anchor fusions to cell-wall material of micro-organisms
CA2462113C (en) * 2001-10-01 2013-01-29 Dyax Corp. Multi-chain eukaryotic display vectors and uses thereof
CA2464396A1 (en) * 2001-10-22 2003-05-01 Daiichi Pharmaceutical Co., Ltd. Method for screening agent acting on cell wall
US20040067532A1 (en) * 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
US7563600B2 (en) * 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
WO2005055944A2 (en) 2003-12-05 2005-06-23 Cincinnati Children's Hospital Medical Center Oligosaccharide compositions and use thereof in the treatment of infection
CA2614181A1 (en) * 2005-07-01 2007-01-11 Medimmune, Inc. An integrated approach for generating multidomain protein therapeutics
JP2009501522A (ja) * 2005-07-12 2009-01-22 コドン デバイシズ インコーポレイテッド 生体触媒工学のための組成物及び方法
KR20080073293A (ko) * 2005-10-14 2008-08-08 메디뮨 엘엘씨 항체 라이브러리의 세포 디스플레이
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
US20080138663A1 (en) * 2006-12-12 2008-06-12 Canon Kabushiki Kaisha Microbial electrode and fuel cell and sensor using the same
WO2008100816A2 (en) * 2007-02-09 2008-08-21 Medimmune, Llc Antibody libray display by yeast cell plasma membrane
US8709980B2 (en) 2007-03-26 2014-04-29 Celexion, Llc Cell surface display, screening and production of proteins of interest
BRPI0816785A2 (pt) 2007-09-14 2017-05-02 Adimab Inc bibliotecas de anticorpos sintéticos racionalmente desenhadas, e, usos para as mesmas
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
EP2379718B2 (en) 2008-12-16 2020-12-30 Novartis AG Yeast display systems
KR102024922B1 (ko) 2010-07-16 2019-09-25 아디맵 엘엘씨 항체 라이브러리
US8759044B2 (en) 2011-03-23 2014-06-24 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8765425B2 (en) 2011-03-23 2014-07-01 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
CN116829685A (zh) 2021-01-28 2023-09-29 诺维信公司 具有低恶臭产生的脂肪酶
AU2023256853A1 (en) 2022-04-20 2024-08-22 Novozymes A/S Process for producing free fatty acids

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US5348867A (en) * 1991-11-15 1994-09-20 George Georgiou Expression of proteins on bacterial surface

Also Published As

Publication number Publication date
FI950042A0 (fi) 1995-01-04
OA10123A (en) 1996-12-18
DE69332829D1 (de) 2003-05-08
DK0673427T3 (da) 2003-07-21
ES2196009T3 (es) 2003-12-16
EP0673427A1 (en) 1995-09-27
US6027910A (en) 2000-02-22
RU95105899A (ru) 1997-03-20
FI950042A (fi) 1995-01-24
AU685057B2 (en) 1998-01-15
JP3681385B2 (ja) 2005-08-10
CA2139670C (en) 2005-09-27
EP0673427B1 (en) 2003-04-02
ATE236257T1 (de) 2003-04-15
DE69332829T2 (de) 2004-01-22
CA2139670A1 (en) 1994-01-20
AU4565393A (en) 1994-01-31
KR950702634A (ko) 1995-07-29
WO1994001567A1 (en) 1994-01-20
NZ254108A (en) 1995-12-21
JP2004254700A (ja) 2004-09-16
JPH07508652A (ja) 1995-09-28

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