DE69332829D1 - Enzymatisches verfahren dass enzyme die an der zellwand einer eukaryontischen mikrobiellen zelle durch schaffung eines fusionsproteins immobilisiert wurden verwendet. - Google Patents

Enzymatisches verfahren dass enzyme die an der zellwand einer eukaryontischen mikrobiellen zelle durch schaffung eines fusionsproteins immobilisiert wurden verwendet.

Info

Publication number
DE69332829D1
DE69332829D1 DE69332829T DE69332829T DE69332829D1 DE 69332829 D1 DE69332829 D1 DE 69332829D1 DE 69332829 T DE69332829 T DE 69332829T DE 69332829 T DE69332829 T DE 69332829T DE 69332829 D1 DE69332829 D1 DE 69332829D1
Authority
DE
Germany
Prior art keywords
cell wall
pct
enzyme
immobilized
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69332829T
Other languages
English (en)
Other versions
DE69332829T2 (de
Inventor
Maria Klis
Pleun Schreuder
York Toschka
Theodorus Verrips
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever NV
Original Assignee
Unilever NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever NV filed Critical Unilever NV
Publication of DE69332829D1 publication Critical patent/DE69332829D1/de
Application granted granted Critical
Publication of DE69332829T2 publication Critical patent/DE69332829T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/16—Enzymes or microbial cells immobilised on or in a biological cell
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69332829T 1992-07-08 1993-07-07 Enzymatisches verfahren dass enzyme die an der zellwand einer eukaryontischen mikrobiellen zelle durch schaffung eines fusionsproteins immobilisiert wurden verwendet. Expired - Lifetime DE69332829T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP92202080 1992-07-08
EP92202080 1992-07-08
EP92203899 1992-12-14
EP92203899 1992-12-14
PCT/EP1993/001763 WO1994001567A1 (en) 1992-07-08 1993-07-07 Process for immobilizing enzymes to the cell wall of a microbial cell by producing a fusion protein

Publications (2)

Publication Number Publication Date
DE69332829D1 true DE69332829D1 (de) 2003-05-08
DE69332829T2 DE69332829T2 (de) 2004-01-22

Family

ID=26131543

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69332829T Expired - Lifetime DE69332829T2 (de) 1992-07-08 1993-07-07 Enzymatisches verfahren dass enzyme die an der zellwand einer eukaryontischen mikrobiellen zelle durch schaffung eines fusionsproteins immobilisiert wurden verwendet.

Country Status (16)

Country Link
US (1) US6027910A (de)
EP (1) EP0673427B1 (de)
JP (2) JP3681385B2 (de)
KR (1) KR950702634A (de)
AT (1) ATE236257T1 (de)
AU (1) AU685057B2 (de)
CA (1) CA2139670C (de)
DE (1) DE69332829T2 (de)
DK (1) DK0673427T3 (de)
ES (1) ES2196009T3 (de)
FI (1) FI950042A (de)
NZ (1) NZ254108A (de)
OA (1) OA10123A (de)
PT (1) PT673427E (de)
RU (1) RU95105899A (de)
WO (1) WO1994001567A1 (de)

Families Citing this family (39)

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Publication number Priority date Publication date Assignee Title
US5866344A (en) * 1991-11-15 1999-02-02 Board Of Regents, The University Of Texas System Antibody selection methods using cell surface expressed libraries
US20030036092A1 (en) * 1991-11-15 2003-02-20 Board Of Regents, The University Of Texas System Directed evolution of enzymes and antibodies
US6114147A (en) * 1993-02-10 2000-09-05 Unilever Patent Holdings Immobilized proteins with specific binding capacities and their use in processes and products
RU2159807C2 (ru) * 1993-02-26 2000-11-27 Саппоро Бревериз Лтд. Гены агглютинации, дрожжи
GB9423367D0 (en) * 1994-11-18 1995-01-11 Wellcome Found Enzyme prodrug therapy
US6696251B1 (en) 1996-05-31 2004-02-24 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6699658B1 (en) 1996-05-31 2004-03-02 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6300065B1 (en) 1996-05-31 2001-10-09 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6759243B2 (en) 1998-01-20 2004-07-06 Board Of Trustees Of The University Of Illinois High affinity TCR proteins and methods
IL145337A0 (en) 1999-03-25 2002-06-30 Valtion Teknillinen Process for partitioning of proteins
JP2003284556A (ja) * 1999-11-19 2003-10-07 Toyota Motor Corp 金属捕捉能タンパク質を発現する遺伝子
EP1134231B1 (de) 2000-03-14 2009-04-15 Unilever N.V. Variabele Domänen der schweren Kette eines Antikörpers gegen menschliche Ernährungslipasen und deren Verwendungen
DE10016279A1 (de) * 2000-04-03 2001-10-04 Henkel Kgaa Enzymatisches Färbemittel
AU2001252604A1 (en) * 2000-05-01 2001-11-12 Daiichi Pharmaceutical Co., Ltd. Method of screening drug acting on cell wall
WO2001087797A1 (en) * 2000-05-15 2001-11-22 Services Petroliers Schlumberger (Sps) Permeable cements
WO2002004626A1 (fr) 2000-07-07 2002-01-17 Eisai Co., Ltd. Gene de synthese de la paroi des cellules fongiques
AU2002220275A1 (en) * 2000-12-08 2002-06-18 The Board Of Trustees Of The University Of Illinois Mutated class ii major histocompatibility proteins
ATE416192T1 (de) * 2001-04-19 2008-12-15 Kansai Chem Eng Protein, das sich an die zelloberflächenschichten bindet, und dessen verwendung
ATE429484T1 (de) 2001-06-11 2009-05-15 Applied Nanosystems Bv Verfahren zur bindung von acma-typ protein anker fusionen an mikroorganismen zellwandmaterialien
WO2003029456A1 (en) 2001-10-01 2003-04-10 Dyax Corp. Multi-chain eukaryotic display vectors and uses thereof
ES2294172T3 (es) 2001-10-22 2008-04-01 Daiichi Sankyo Company, Limited Procedimiento para cribar un medicamento que actua sobre la pared celular.
US20040067532A1 (en) * 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
US7563600B2 (en) * 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
EP1689348B1 (de) 2003-12-05 2013-05-15 Children's Hospital Medical Center Oligosaccharid-Zusammensetzungen und ihre Verwendung bei der Behandlung von Infektionen
US8309690B2 (en) * 2005-07-01 2012-11-13 Medimmune, Llc Integrated approach for generating multidomain protein therapeutics
JP2009501522A (ja) * 2005-07-12 2009-01-22 コドン デバイシズ インコーポレイテッド 生体触媒工学のための組成物及び方法
AU2006304387A1 (en) * 2005-10-14 2007-04-26 Medimmune, Llc Cell display of antibody libraries
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
US20080138663A1 (en) * 2006-12-12 2008-06-12 Canon Kabushiki Kaisha Microbial electrode and fuel cell and sensor using the same
US20110076752A1 (en) * 2007-02-09 2011-03-31 Medimmune, Llc Antibody library display by yeast cell plasma membrane
EP2137308B1 (de) 2007-03-26 2016-08-03 Agenus Inc. Zelloberflächenpräsentation, screening und produktion von interessierenden proteinen
CA2697193C (en) 2007-09-14 2017-06-06 Adimab, Inc. Rationally designed, synthetic antibody libraries and uses therefor
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US20110275535A1 (en) 2008-12-16 2011-11-10 Novartis Ag Yeast Display Systems
AU2011279073B2 (en) 2010-07-16 2016-06-09 Adimab, Llc Antibody libraries
US8759044B2 (en) 2011-03-23 2014-06-24 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8765425B2 (en) 2011-03-23 2014-07-01 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
WO2022162043A1 (en) 2021-01-28 2022-08-04 Novozymes A/S Lipase with low malodor generation
WO2023203080A1 (en) 2022-04-20 2023-10-26 Novozymes A/S Process for producing free fatty acids

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WO1989007140A1 (en) * 1988-02-05 1989-08-10 Commonwealth Scientific And Industrial Research Or Gene expression system (particularly for rotavirus vp7 protein) involving a foreign signal peptide and optionally a transmembrane anchor sequence
CA2074089C (en) * 1990-01-23 2001-04-10 Tse-Wen Chang Extracellular segments of human e immunoglobulin anchoring peptides and antibodies specific therefor
US5348867A (en) * 1991-11-15 1994-09-20 George Georgiou Expression of proteins on bacterial surface

Also Published As

Publication number Publication date
EP0673427A1 (de) 1995-09-27
KR950702634A (ko) 1995-07-29
FI950042A0 (fi) 1995-01-04
JP3681385B2 (ja) 2005-08-10
JP2004254700A (ja) 2004-09-16
OA10123A (en) 1996-12-18
FI950042A (fi) 1995-01-24
AU4565393A (en) 1994-01-31
PT673427E (pt) 2003-08-29
RU95105899A (ru) 1997-03-20
JPH07508652A (ja) 1995-09-28
CA2139670A1 (en) 1994-01-20
EP0673427B1 (de) 2003-04-02
ATE236257T1 (de) 2003-04-15
US6027910A (en) 2000-02-22
WO1994001567A1 (en) 1994-01-20
AU685057B2 (en) 1998-01-15
CA2139670C (en) 2005-09-27
DE69332829T2 (de) 2004-01-22
ES2196009T3 (es) 2003-12-16
NZ254108A (en) 1995-12-21
DK0673427T3 (da) 2003-07-21

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Representative=s name: MEISSNER, BOLTE & PARTNER GBR, 80538 MUENCHEN